##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938001_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2309201 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.80877238490716 33.0 31.0 34.0 30.0 34.0 2 32.00902519962533 34.0 31.0 34.0 30.0 34.0 3 32.08044947148386 34.0 31.0 34.0 30.0 34.0 4 35.601603758183025 37.0 35.0 37.0 33.0 37.0 5 35.62014653553329 37.0 35.0 37.0 33.0 37.0 6 35.6467553062726 37.0 35.0 37.0 33.0 37.0 7 35.584464063544054 37.0 35.0 37.0 33.0 37.0 8 35.562383265900195 37.0 35.0 37.0 33.0 37.0 9 37.21658876814968 39.0 37.0 39.0 34.0 39.0 10-11 37.132393412266836 39.0 37.0 39.0 34.0 39.0 12-13 37.03193182403784 39.0 37.0 39.0 33.0 39.0 14-15 38.266241007170876 40.0 38.0 41.0 33.0 41.0 16-17 38.17743539865087 40.0 38.0 41.0 33.0 41.0 18-19 38.1183645338799 40.0 38.0 41.0 33.0 41.0 20-21 38.00497249914581 40.0 38.0 41.0 33.0 41.0 22-23 37.84357836325205 40.0 38.0 41.0 33.0 41.0 24-25 37.79060289684614 40.0 38.0 41.0 32.5 41.0 26-27 37.79278806825391 40.0 38.0 41.0 33.0 41.0 28-29 37.69799185952198 40.0 38.0 41.0 32.0 41.0 30-31 37.49324246784927 40.0 37.0 41.0 32.0 41.0 32-33 37.253482481602944 40.0 37.0 41.0 31.5 41.0 34-35 37.03282477359052 40.0 36.0 41.0 31.0 41.0 36-37 36.79671128671779 40.0 36.0 41.0 30.0 41.0 38-39 36.53134006091285 39.0 36.0 41.0 30.0 41.0 40-41 36.29251676229137 39.0 35.0 41.0 30.0 41.0 42-43 35.9882175696269 39.0 35.0 40.5 28.5 41.0 44-45 35.73804012729944 39.0 35.0 40.5 27.5 41.0 46-47 35.571018287277724 39.0 35.0 41.0 26.5 41.0 48-49 35.2893693532958 39.0 35.0 41.0 25.5 41.0 50-51 34.641200138056405 38.5 34.0 39.5 24.0 40.5 52-53 34.7201296032697 38.5 34.5 40.0 24.0 41.0 54-55 34.84526141293027 39.0 35.0 40.0 24.0 41.0 56-57 34.61645478241175 38.0 34.0 40.0 23.5 41.0 58-59 34.339288784302454 38.0 34.0 40.0 23.0 41.0 60-61 33.947556319263676 38.0 34.0 40.0 22.0 41.0 62-63 33.51336869332726 37.0 33.0 40.0 20.5 41.0 64-65 33.07134242536704 36.5 33.0 39.5 18.5 41.0 66-67 32.62126791907677 36.0 32.0 39.0 16.0 41.0 68-69 32.113645802162736 35.5 31.5 39.0 10.5 40.0 70-71 31.619132981494467 35.0 31.0 38.0 7.5 40.0 72-73 31.109850766563845 35.0 31.0 37.5 6.0 39.5 74-75 30.571131529910126 35.0 30.0 37.0 2.0 39.0 76-77 30.01894551405443 34.5 29.5 36.0 2.0 39.0 78-79 29.429022202917807 34.0 29.0 36.0 2.0 38.5 80-81 28.873588526940704 34.0 28.5 35.0 2.0 37.0 82-83 28.31628255833944 34.0 27.5 35.0 2.0 37.0 84-85 27.79861843988462 34.0 26.5 35.0 2.0 36.0 86-87 27.270756205284858 33.0 26.0 35.0 2.0 36.0 88-89 26.677536515877136 33.0 25.0 35.0 2.0 35.0 90-91 26.033871672496247 32.0 23.5 35.0 2.0 35.0 92-93 25.38107553218624 32.0 20.0 34.5 2.0 35.0 94-95 24.73622824518091 32.0 17.0 34.0 2.0 35.0 96-97 23.84798313356005 31.0 4.5 34.0 2.0 35.0 98-99 22.71665091085618 31.0 2.0 34.0 2.0 35.0 100-101 19.838584861170595 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3855314418113558 1101 2 0.3548673378953495 1101 3 0.39467172321340627 1101 4 0.20869340784133072 1101 5 0.20313622804676612 1101 6 0.2757910992901387 1101 7 0.2989720074449238 1101 8 0.3675721196879991 1101 9 0.36797433481135045 1101 10-11 0.3702707183131082 1101 12-13 0.30664466905866306 1101 14-15 0.5138128461790998 1101 16-17 0.6097089746321487 1101 18-19 0.5427319474192629 1101 20-21 0.673594726468906 1101 22-23 0.639331176821301 1101 24-25 0.6345486747723115 1101 26-27 0.5626390860074366 1101 28-29 0.6487127147094682 1101 30-31 0.7582997760261705 1101 32-33 0.686539147319543 1101 34-35 0.6135144328040241 1101 36-37 0.6240865491753027 1101 38-39 0.8229433128214723 1101 40-41 0.8535930916799188 1101 42-43 0.9221617506892414 1101 44-45 1.0485961127720032 1101 46-47 0.9459905548234531 1101 48-49 1.0979730023291339 1101 50-51 1.1408251938503966 1101 52-53 1.13822975839431 1101 54-55 1.1714700856172868 1101 56-57 1.062049768782387 1101 58-59 1.0953716715687989 1101 60-61 1.066308641342573 1101 62-63 1.1196434896938001 1101 64-65 1.0944741239541358 1101 66-67 0.9829533885606381 1101 68-69 1.0804383591560693 1101 70-71 1.142781724021134 1101 72-73 1.2119923559652541 1101 74-75 1.3762304199289588 1101 76-77 1.4724581596913247 1101 78-79 1.4751941587150235 1101 80-81 1.5781043609735832 1101 82-83 1.5701967145394349 1101 84-85 1.6360963619184865 1101 86-87 1.7168941347998476 1101 88-89 1.8684911332192726 1101 90-91 1.8828374011123081 1101 92-93 1.8772174785711933 1101 94-95 2.114303535389041 1101 96-97 2.370432853945669 1101 98-99 2.5801430944710937 1101 100-101 2.5931072810508056 1102 1 0.5381590282152651 1102 2 0.48914461541726695 1102 3 0.4754675056174449 1102 4 0.30345241218228836 1102 5 0.28158960691625623 1102 6 0.4709943562255674 1102 7 0.26750148797938067 1102 8 0.30144926305599995 1102 9 0.47283212425529797 1102 10-11 0.5383070668297449 1102 12-13 0.4716316609956763 1102 14-15 0.5954565067189108 1102 16-17 0.59721753117622 1102 18-19 0.6462739070186316 1102 20-21 0.7204593019003269 1102 22-23 0.6534578591674673 1102 24-25 0.6858328176314146 1102 26-27 0.7665522964650862 1102 28-29 0.7479719057867484 1102 30-31 0.6863192177639021 1102 32-33 0.7774214420709313 1102 34-35 0.6967749231006124 1102 36-37 0.793394503296355 1102 38-39 0.7267333725242011 1102 40-41 0.868580453915591 1102 42-43 0.8599740050357454 1102 44-45 0.8036567729827837 1102 46-47 0.8356657817371769 1102 48-49 0.8274483526052236 1102 50-51 0.8798226155137172 1102 52-53 0.951412073353417 1102 54-55 0.8094634689092004 1102 56-57 0.8066289666731663 1102 58-59 0.8998509099808345 1102 60-61 0.932640769618267 1102 62-63 0.982393560315046 1102 64-65 0.974128751682322 1102 66-67 0.8334622318216773 1102 68-69 0.9151549166904331 1102 70-71 0.9085807280235372 1102 72-73 1.1178258294830847 1102 74-75 1.1161649088057146 1102 76-77 0.9931341557682778 1102 78-79 1.1006529323070176 1102 80-81 1.2034298270473514 1102 82-83 1.1999363522603836 1102 84-85 1.3634668288016236 1102 86-87 1.3746871759357937 1102 88-89 1.2883005246281343 1102 90-91 1.5813192823987912 1102 92-93 1.5946532555582955 1102 94-95 1.6034756851208947 1102 96-97 1.535612295387164 1102 98-99 1.697139591445751 1102 100-101 1.3015624690937564 1103 1 0.3471285133326951 1103 2 0.40062294084133754 1103 3 0.3038567814681983 1103 4 0.20182790981442622 1103 5 0.22887778063243047 1103 6 0.17511172059123226 1103 7 0.2052819533906458 1103 8 0.2394153226698421 1103 9 0.3445865302739577 1103 10-11 0.30991066219505115 1103 12-13 0.26348807007970976 1103 14-15 0.405395137721996 1103 16-17 0.3813969774172037 1103 18-19 0.38465153099493676 1103 20-21 0.3935544723950315 1103 22-23 0.3322907789084226 1103 24-25 0.5178252021236105 1103 26-27 0.443702577376051 1103 28-29 0.5322121925113592 1103 30-31 0.5176085566675184 1103 32-33 0.6330521981030515 1103 34-35 0.7725548182091657 1103 36-37 0.6761005036429637 1103 38-39 0.7658882183150837 1103 40-41 0.828513615420654 1103 42-43 0.7829124651938386 1103 44-45 0.8596297106440218 1103 46-47 0.9158633719623879 1103 48-49 0.9309398589049707 1103 50-51 0.8552777822227853 1103 52-53 0.9736369791121291 1103 54-55 1.075356969793539 1103 56-57 1.006926286127495 1103 58-59 1.0458886581208944 1103 60-61 1.1296570048091397 1103 62-63 1.0944303890878047 1103 64-65 1.0761484099185807 1103 66-67 1.0712434346933506 1103 68-69 1.0886354102481022 1103 70-71 1.1644893222642168 1103 72-73 1.2183997580452974 1103 74-75 0.9702688145189455 1103 76-77 1.1433490025670245 1103 78-79 1.1930951307748217 1103 80-81 1.2188341458613152 1103 82-83 1.3978123433764438 1103 84-85 1.4207911626597785 1103 86-87 1.462358620116806 1103 88-89 1.405743245463725 1103 90-91 1.4500304351354458 1103 92-93 1.447705051090022 1103 94-95 1.4300170081002825 1103 96-97 1.611459176482736 1103 98-99 1.7210283922490923 1103 100-101 1.717753781072691 1104 1 0.3401177695701243 1104 2 0.3243928755799459 1104 3 0.2801443739876248 1104 4 0.22469733974352835 1104 5 0.2187602913689659 1104 6 0.19744613138822587 1104 7 0.28422717235572037 1104 8 0.27559770127345473 1104 9 0.25865049886532177 1104 10-11 0.4036437474420822 1104 12-13 0.30562102213863795 1104 14-15 0.2385606180843709 1104 16-17 0.3717485580685107 1104 18-19 0.3029906698134468 1104 20-21 0.3403314999475455 1104 22-23 0.36226670109342507 1104 24-25 0.46641060606695817 1104 26-27 0.42007321705805367 1104 28-29 0.4849946046464808 1104 30-31 0.40526430917838496 1104 32-33 0.4533702824140917 1104 34-35 0.6070813927149743 1104 36-37 0.6100312098721261 1104 38-39 0.5120263298499594 1104 40-41 0.5169122589966904 1104 42-43 0.5872977071072896 1104 44-45 0.6678025870889925 1104 46-47 0.573543944827577 1104 48-49 0.6880061954152055 1104 50-51 0.7739663053394068 1104 52-53 0.7354169748679311 1104 54-55 0.7295297282138336 1104 56-57 0.7208848922951816 1104 58-59 0.8562414737323607 1104 60-61 0.7702732421122036 1104 62-63 0.6404828741129336 1104 64-65 0.7319239785766953 1104 66-67 0.6979540596887048 1104 68-69 0.7063719588549233 1104 70-71 0.8039524741404733 1104 72-73 0.836531220335825 1104 74-75 0.883045649792848 1104 76-77 0.9016529372048048 1104 78-79 0.6829718770612452 1104 80-81 0.8469143651214601 1104 82-83 0.8236334453282659 1104 84-85 0.9066347479210783 1104 86-87 0.9070543880724777 1104 88-89 1.0155510372025454 1104 90-91 1.0594930437855616 1104 92-93 0.9605658323899142 1104 94-95 0.9669128106817375 1104 96-97 1.0039387967090398 1104 98-99 1.253233886493046 1104 100-101 1.3022655047917837 1105 1 0.37041271741979287 1105 2 0.2831390464405388 1105 3 0.287203687472676 1105 4 0.23930109900981478 1105 5 0.25762011016158937 1105 6 0.2518240755452581 1105 7 0.262142269576529 1105 8 0.3731998039452904 1105 9 0.33339270870678916 1105 10-11 0.3783079400142526 1105 12-13 0.33612632971917833 1105 14-15 0.40430205978427836 1105 16-17 0.4148010415719696 1105 18-19 0.3665020022151424 1105 20-21 0.4194618341353973 1105 22-23 0.46776974126815674 1105 24-25 0.48671434589834206 1105 26-27 0.43137285704954564 1105 28-29 0.5104395251947338 1105 30-31 0.5409827991786571 1105 32-33 0.5759842773980353 1105 34-35 0.525688002434272 1105 36-37 0.5022837155869126 1105 38-39 0.4931926093609391 1105 40-41 0.47993262863010244 1105 42-43 0.6348531660024648 1105 44-45 0.6628835195943381 1105 46-47 0.7974660941431893 1105 48-49 0.6586078446058821 1105 50-51 0.6850711335255681 1105 52-53 0.5608022024877144 1105 54-55 0.5441442229525322 1105 56-57 0.8108753599360625 1105 58-59 0.7001418744616288 1105 60-61 0.7956920643568424 1105 62-63 0.6718565518451527 1105 64-65 0.6263342216981371 1105 66-67 0.874284286521835 1105 68-69 0.7712839927642037 1105 70-71 0.7495729566814475 1105 72-73 0.7307952389192955 1105 74-75 0.8229984507331238 1105 76-77 0.7094769680045019 1105 78-79 0.836840996845055 1105 80-81 0.8241072241813683 1105 82-83 0.9216268374599075 1105 84-85 0.995940366460129 1105 86-87 1.0993949986987452 1105 88-89 1.1342659271176814 1105 90-91 1.2078687483009354 1105 92-93 1.3595371705295314 1105 94-95 1.3991986333798572 1105 96-97 1.5000690290949983 1105 98-99 1.7183994996643612 1105 100-101 1.5329083374757424 1106 1 0.10923518348027628 1106 2 0.03707169164381696 1106 3 0.16250078250331512 1106 4 0.13632490638160277 1106 5 0.10529161543102816 1106 6 0.20183040248537054 1106 7 0.2241502595980407 1106 8 0.1562020274128173 1106 9 0.25722972672953404 1106 10-11 -0.3194987810038441 1106 12-13 -0.15391542808556125 1106 14-15 0.057734334409630605 1106 16-17 0.1970982631414131 1106 18-19 0.24539858385978874 1106 20-21 0.1843941358762038 1106 22-23 0.06298277838018151 1106 24-25 0.12617836368607982 1106 26-27 0.10852573399959198 1106 28-29 -0.07575509148308157 1106 30-31 0.12901457849498854 1106 32-33 0.21450576187895365 1106 34-35 0.028311874883165444 1106 36-37 -0.10488344217082357 1106 38-39 0.10332059432089835 1106 40-41 0.3084134399245997 1106 42-43 0.2654054174892977 1106 44-45 0.35562398851099175 1106 46-47 0.6272609205138764 1106 48-49 0.5906355385265911 1106 50-51 0.6001178624975338 1106 52-53 0.6711295764460274 1106 54-55 0.3112464843816909 1106 56-57 0.49849136890224344 1106 58-59 0.7441762930158333 1106 60-61 0.44643882405966195 1106 62-63 0.5499823314145615 1106 64-65 0.5579566299123044 1106 66-67 0.33854647492004375 1106 68-69 0.22106851526099547 1106 70-71 0.5621691713635641 1106 72-73 0.6112128169666171 1106 74-75 0.684184300079643 1106 76-77 0.6476152206844112 1106 78-79 0.7226630471351889 1106 80-81 0.6645687292700302 1106 82-83 0.7240086592571195 1106 84-85 0.8322078333340777 1106 86-87 0.7721694523222773 1106 88-89 0.5569190842607981 1106 90-91 0.5078584444270398 1106 92-93 0.6187491953311337 1106 94-95 0.5525737847744985 1106 96-97 0.6790169493393989 1106 98-99 0.6595106911175677 1106 100-101 0.47581312606556025 1107 1 0.18658639825351386 1107 2 0.1130875456342153 1107 3 0.15382284161094617 1107 4 0.1995476786279795 1107 5 0.08764733449355333 1107 6 0.05181887344360803 1107 7 0.08844097425322417 1107 8 0.20933186796582248 1107 9 0.16816078167493487 1107 10-11 0.08631015957762145 1107 12-13 0.008287802954363599 1107 14-15 0.1502959213043269 1107 16-17 0.2056401405679651 1107 18-19 0.24424706455366163 1107 20-21 0.21384512498489272 1107 22-23 0.2564489276594557 1107 24-25 0.24782100632248216 1107 26-27 0.30972032275902706 1107 28-29 0.29570630630762906 1107 30-31 0.3129457255342416 1107 32-33 0.2463465170761978 1107 34-35 0.3454074357520014 1107 36-37 0.3355931192526569 1107 38-39 0.5138825814429921 1107 40-41 0.5210245497662456 1107 42-43 0.6201098897188402 1107 44-45 0.6655272276523974 1107 46-47 0.5836366537159279 1107 48-49 0.4295218538656158 1107 50-51 0.5006864554489638 1107 52-53 0.4448594949672042 1107 54-55 0.35964006994138487 1107 56-57 0.4554760986344988 1107 58-59 0.44793427592515656 1107 60-61 0.40380318124806536 1107 62-63 0.44032037122818224 1107 64-65 0.3690065413152581 1107 66-67 0.5104713194164603 1107 68-69 0.5258120754429427 1107 70-71 0.7109171797475682 1107 72-73 0.6805007999659587 1107 74-75 0.5994934480453722 1107 76-77 0.7201997133269451 1107 78-79 0.5464387009841936 1107 80-81 0.6666524391148627 1107 82-83 0.6970860963338232 1107 84-85 0.6607382648110622 1107 86-87 0.7536279488105926 1107 88-89 0.8569895741377422 1107 90-91 0.9780681621974843 1107 92-93 1.0672848339188725 1107 94-95 0.9844361093201393 1107 96-97 1.0342289763803656 1107 98-99 0.8458523772935891 1107 100-101 0.717726870829889 1108 1 0.22418052617190298 1108 2 0.19318170695407844 1108 3 0.16898505457668023 1108 4 0.1473745788377414 1108 5 0.17230516467224533 1108 6 0.22462061977535797 1108 7 0.22771997521004295 1108 8 0.1438418580892673 1108 9 0.21713145107127474 1108 10-11 0.29403160363118275 1108 12-13 0.21199952872108696 1108 14-15 0.18868705973955713 1108 16-17 0.3839374568367404 1108 18-19 0.3864425279142978 1108 20-21 0.4222327999443749 1108 22-23 0.3660792401636357 1108 24-25 0.19595674168416366 1108 26-27 0.2835710808696703 1108 28-29 0.17666885452137393 1108 30-31 0.24044448967976706 1108 32-33 0.27678373721040117 1108 34-35 0.2977162996805731 1108 36-37 0.37945130790622983 1108 38-39 0.41800314123111804 1108 40-41 0.3046038207097368 1108 42-43 0.3156308036653499 1108 44-45 0.23036154093353645 1108 46-47 0.15326407827741662 1108 48-49 0.24664930668989626 1108 50-51 0.24466339918340907 1108 52-53 0.10983630406803258 1108 54-55 0.07540767174649687 1108 56-57 0.2211627147159163 1108 58-59 0.2747586900376362 1108 60-61 0.18565405446532424 1108 62-63 0.07351002684359287 1108 64-65 0.16859308247460802 1108 66-67 0.38440645478491575 1108 68-69 0.3541434098266265 1108 70-71 0.3935613122393917 1108 72-73 0.34540078568040755 1108 74-75 0.2558182480733251 1108 76-77 0.39710264659285954 1108 78-79 0.33085403647372047 1108 80-81 0.5325398751253516 1108 82-83 0.5188733114834996 1108 84-85 0.5451874128679712 1108 86-87 0.8326989973393317 1108 88-89 0.7576728654879972 1108 90-91 0.8034547713930635 1108 92-93 0.886635540128772 1108 94-95 0.7443339933111695 1108 96-97 0.6878641870819955 1108 98-99 0.9122200750893974 1108 100-101 0.8142070236750243 1109 1 0.3440122190841528 1109 2 0.3169655244144991 1109 3 0.15343249360842748 1109 4 0.1847010328246128 1109 5 0.22414908565360037 1109 6 0.28663165180423533 1109 7 0.33110880711716817 1109 8 0.23107876289100204 1109 9 0.23357296605043132 1109 10-11 0.38796844886165616 1109 12-13 0.24778699429646878 1109 14-15 0.2900008003188148 1109 16-17 0.37516973207828386 1109 18-19 0.31305978773615806 1109 20-21 0.24303174712131437 1109 22-23 0.41361097464886143 1109 24-25 0.38208021380046375 1109 26-27 0.3329132202895053 1109 28-29 0.27827449044749386 1109 30-31 0.3228679094548852 1109 32-33 0.14905721738362843 1109 34-35 0.3567765543409962 1109 36-37 0.30174897808910117 1109 38-39 0.353717613740848 1109 40-41 0.32577113673779223 1109 42-43 0.32853111659715495 1109 44-45 0.4139568878371236 1109 46-47 0.4551431882235093 1109 48-49 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3386.0 8 4140.0 9 4971.0 10 6438.0 11 8026.0 12 9901.0 13 11904.0 14 14115.0 15 16492.0 16 20029.0 17 25753.0 18 23437.0 19 19285.0 20 18792.0 21 19231.0 22 20446.0 23 22151.0 24 24678.0 25 27423.0 26 31102.0 27 35495.0 28 41394.0 29 48930.0 30 58195.0 31 71236.0 32 88207.0 33 114108.0 34 156301.0 35 225724.0 36 332754.0 37 440619.0 38 313662.0 39 34060.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.46311898144481 18.523549344862612 14.971192756538535 23.04213891715404 2 15.303027677163309 19.566775272525604 40.921387844231475 24.208809206079618 3 19.36534416007869 23.706325976606085 29.672749489246552 27.25558037406867 4 12.72390211856309 16.63446217340679 37.09619617194816 33.545439536081965 5 13.761296119002184 37.04368576690089 34.29083711441721 14.904180999679713 6 32.29302411478126 36.01437193449741 17.24887914344103 14.4437248072803 7 28.49135114240497 32.843167844519904 20.234071246687016 18.431409766388107 8 26.337738120873354 34.92185289635831 19.37830667771799 19.36210230505035 9 25.981155267785923 17.075379362128302 20.258672047327167 36.68479332275861 10-11 24.961759693340802 26.582251600523954 26.950450660307546 21.505538045827695 12-13 26.080939951176436 23.787268503164512 27.462865347624042 22.668926198035006 14-15 22.688855708545248 25.3416487357047 26.00501489215859 25.964480663591466 16-17 22.05072340838564 28.455290894039127 26.8954872636836 22.59849843389164 18-19 22.004668075076584 26.69761528657011 29.490097402140176 21.807619236213135 20-21 22.508400621733767 25.91216114458647 29.920298376735733 21.659139856944027 22-23 22.23755517479566 25.89800690394537 29.804334769416375 22.060103151842597 24-25 22.122029815139374 26.03574635264409 29.823829875951212 22.01839395626532 26-27 21.646438570999553 26.34053836910139 29.71421811155231 22.298804948346746 28-29 21.831869401919484 26.792826656624484 29.493404183309146 21.88189975814689 30-31 21.944482725445482 26.237379187327576 29.469516333275266 22.348621753951683 32-33 21.625117849072147 26.295028854739954 29.482343820049913 22.597509476137986 34-35 22.419103095461956 26.45362852679691 29.180828170907514 21.94644020683362 36-37 22.478209639025167 25.979618271617994 29.286102141788913 22.256069947567923 38-39 21.9333745914354 26.433764175071545 29.15881343893501 22.474047794558047 40-41 22.450350951909126 26.23121277594057 29.274709496908997 22.043726775241307 42-43 22.135081132554486 26.249437873180725 29.021318795008057 22.594162199256736 44-45 22.137233971362434 26.560124188386748 29.131493420358762 22.17114841989206 46-47 22.616117061574627 26.81028780683679 28.364521828625783 22.209073302962796 48-49 22.314112950297353 26.82063302881249 28.364815984756124 22.500438036134028 50-51 22.438381839300174 27.084138734017333 28.313069181401033 22.164410245281463 52-53 22.723935452739585 26.733016147210865 28.27451211408998 22.268536285959577 54-55 22.29780333019872 26.98616626043746 28.38760286940615 22.328427539957662 56-57 22.373603554317302 27.132098747784937 28.146445357829258 22.3478523400685 58-59 22.350938768102523 26.750380789456184 28.162804845430777 22.735875597010512 60-61 22.061764345964374 26.99810377371772 28.42593725667713 22.514194623640776 62-63 22.143119962248058 26.962577890989326 28.611409913262793 22.282892233499823 64-65 21.931953612946135 27.05928801195649 28.36894024332694 22.639818131770436 66-67 21.862669684629786 27.07996043038025 28.277573779409764 22.7797961055802 68-69 22.381813586708297 26.779398716680703 28.39412702431177 22.44466067229923 70-71 22.294982393145805 26.880714429302245 28.017426626554464 22.80687655099749 72-73 22.24397727048731 26.976490901328326 27.988328088077836 22.791203740106525 74-75 22.265830768804236 27.386289992791596 27.837725089411958 22.51015414899221 76-77 22.417814320388086 27.034562697530752 27.93317940562478 22.61444357645638 78-79 22.473621386664867 27.2816276996879 27.83455860378937 22.410192309857862 80-81 22.655725520803106 27.266681334939914 27.556978878552762 22.520614265704218 82-83 22.587655704889407 27.344525756426304 27.657879240770217 22.409939297914075 84-85 22.56013326348493 27.516085670991913 27.576441451752515 22.347339613770647 86-87 22.761785249098356 27.45918918655552 27.313583610061166 22.46544195428496 88-89 22.67964370196432 27.54793820239323 27.146648008387558 22.625770087254892 90-91 22.576404464927723 27.567590169151718 27.228883765902186 22.62712160001837 92-93 22.732318603791295 27.207693062472128 27.455487443387973 22.604500890348604 94-95 22.617765521221745 27.035281939424504 27.51682766937023 22.83012486998352 96-97 22.884316358126984 26.74755029068974 27.47497867322089 22.893154677962386 98-99 22.66525325706582 27.006267636934524 27.13486509504992 23.193614010949737 100-101 23.21996768211346 26.321855784142546 27.418039453849406 23.04013707989459 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 934.0 1 611.0 2 429.0 3 580.0 4 504.5 5 462.0 6 850.5 7 1982.5 8 3377.0 9 3946.0 10 3235.0 11 2399.5 12 2260.0 13 2387.0 14 2573.0 15 2763.5 16 2909.0 17 3089.5 18 3343.0 19 3729.5 20 4234.5 21 5003.5 22 6165.5 23 7736.0 24 9643.5 25 11954.5 26 15092.0 27 18826.5 28 21999.0 29 24993.5 30 28445.0 31 33014.0 32 39718.5 33 42699.5 34 43024.5 35 45965.0 36 50241.5 37 54573.0 38 57909.5 39 60487.0 40 64280.5 41 69225.5 42 71373.0 43 73394.5 44 76937.5 45 81344.5 46 83289.0 47 83988.5 48 85120.5 49 83374.5 50 81532.0 51 80168.0 52 79053.0 53 79396.5 54 79970.5 55 75933.0 56 69903.0 57 64231.5 58 59617.5 59 53310.5 60 42709.0 61 34998.5 62 29556.0 63 21934.0 64 15384.0 65 11718.5 66 8724.5 67 6472.0 68 4895.5 69 3697.5 70 2858.0 71 2063.0 72 1466.0 73 1134.5 74 835.5 75 576.0 76 382.0 77 235.0 78 155.0 79 108.0 80 63.5 81 38.0 82 27.5 83 16.5 84 16.5 85 16.5 86 13.0 87 10.0 88 4.5 89 4.0 90 4.0 91 3.5 92 3.5 93 2.0 94 2.5 95 2.0 96 1.5 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4986573277943323 2 0.0502771304879913 3 0.05785550932985046 4 0.06720939407180232 5 0.08150005131645101 6 0.10752636951049302 7 0.06985100041096466 8 0.05118653594901439 9 0.05118653594901439 10-11 0.07593535599542872 12-13 0.10947509549839966 14-15 0.055213902990688124 16-17 0.06108173346538478 18-19 0.060518768179989524 20-21 0.07719120163207967 22-23 0.06647320869859315 24-25 0.0902693182620309 26-27 0.06937464516947638 28-29 0.06937464516947638 30-31 0.07374845238677794 32-33 0.07338035970017336 34-35 0.07545900075394044 36-37 0.07119345609152256 38-39 0.04189760873999275 40-41 0.04547027305115492 42-43 0.04343493701934132 44-45 0.019508912390043136 46-47 0.02615623325990245 48-49 0.02604797070501875 50-51 0.024575599958600397 52-53 0.02455394744762366 54-55 0.0246838625134841 56-57 0.024770472557391066 58-59 0.02210721370725199 60-61 0.02894940717590197 62-63 0.01929238728027573 64-65 0.021500943399903258 66-67 0.015546502881299634 68-69 0.018296371775345672 70-71 0.04369476715106221 72-73 0.040035492795993076 74-75 0.03399444223348249 76-77 0.023449669387809898 78-79 0.013987522090974324 80-81 0.01571972296911356 82-83 0.02020179274129883 84-85 0.016953916094787764 86-87 0.009678672406602976 88-89 0.04421442741450398 90-91 0.056404791094408845 92-93 0.036181345842133276 94-95 0.0571842814895715 96-97 0.04633637349022454 98-99 0.03615969333115653 100-101 0.17218076728703996 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2309201.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.55941923171839 #Duplication Level Percentage of deduplicated Percentage of total 1 77.69814941302357 44.722603555929176 2 12.783842921918692 14.716611482703074 3 4.228864378677915 7.302329329392074 4 1.812520893234911 4.1731059983982774 5 0.9734633204720079 2.801599168487446 6 0.5808271770344567 2.0059244990461087 7 0.3819705755920862 1.5390203146279968 8 0.2559712761596471 1.178684639660087 9 0.18711068983343582 0.9692984374972884 >10 0.9963144074437158 10.233439186368303 >50 0.06387279081531401 2.53084885802311 >100 0.03128078962528442 3.3371253927352527 >500 0.0031698360921434687 1.2147521646311246 >1k 0.0024151132130608424 2.1950510362489513 >5k 1.5094457581630265E-4 0.6098305220143437 >10k+ 7.547228790815132E-5 0.46977541423729263 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10814 0.4683005074049422 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8542 0.36991149752663366 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5496 0.23800440065633088 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2833 0.12268312719421134 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2648 0.11467169813281737 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2615 0.1132426324083525 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2531 0.10960501056426011 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2347 0.10163688652481961 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.330502195348088E-5 2 0.0 0.0 0.0 0.0 4.330502195348088E-5 3 0.0 0.0 0.0 0.0 4.330502195348088E-5 4 0.0 0.0 0.0 0.0 4.330502195348088E-5 5 0.0 0.0 0.0 0.0 4.330502195348088E-5 6 0.0 0.0 0.0 0.0 8.661004390696177E-5 7 0.0 0.0 0.0 0.0 8.661004390696177E-5 8 0.0 0.0 0.0 0.0 8.661004390696177E-5 9 0.0 0.0 0.0 0.0 8.661004390696177E-5 10-11 0.0 0.0 0.0 0.0 8.661004390696177E-5 12-13 0.0 0.0 0.0 0.0 3.897451975813279E-4 14-15 4.330502195348088E-5 0.0 0.0 0.0 5.196602634417705E-4 16-17 4.330502195348088E-5 0.0 0.0 6.495753293022132E-5 6.062703073487323E-4 18-19 4.330502195348088E-5 0.0 0.0 1.2991506586044265E-4 6.928803512556941E-4 20-21 4.330502195348088E-5 0.0 0.0 1.5156757683718308E-4 8.227954171161367E-4 22-23 4.330502195348088E-5 0.0 0.0 3.0313515367436615E-4 8.227954171161367E-4 24-25 4.330502195348088E-5 0.0 0.0 4.1139770855806837E-4 8.227954171161367E-4 26-27 4.330502195348088E-5 0.0 0.0 9.743629939533197E-4 9.094054610230985E-4 28-29 4.330502195348088E-5 0.0 0.0 0.0032911816684645465 9.527104829765794E-4 30-31 4.330502195348088E-5 0.0 0.0 0.009678672406602976 9.527104829765794E-4 32-33 4.330502195348088E-5 0.0 0.0 0.023254796789019233 9.960155049300603E-4 34-35 4.330502195348088E-5 0.0 0.0 0.0443876475023179 9.960155049300603E-4 36-37 4.330502195348088E-5 0.0 0.0 0.07957297783952111 9.960155049300603E-4 38-39 4.330502195348088E-5 0.0 0.0 0.13019654850314027 9.960155049300603E-4 40-41 4.330502195348088E-5 0.0 0.0 0.18443608849987508 9.960155049300603E-4 42-43 4.330502195348088E-5 0.0 0.0 0.2400180841771678 9.960155049300603E-4 44-45 4.330502195348088E-5 0.0 0.0 0.3076172234465514 0.0010176680159068008 46-47 4.330502195348088E-5 0.0 0.0 0.3876665565275609 0.0011042780598137624 48-49 4.330502195348088E-5 0.0 0.0 0.46832215991591897 0.0011692355927439837 50-51 4.330502195348088E-5 0.0 0.0 0.5538062732520903 0.0012125406146974646 52-53 4.330502195348088E-5 0.0 0.0 0.6382943710833314 0.0012991506586044264 54-55 4.330502195348088E-5 0.0 0.0 0.7295813573612692 0.0013857607025113882 56-57 4.330502195348088E-5 0.0 0.0 0.8339031552472045 0.0014074132134881286 58-59 4.330502195348088E-5 0.0 0.0 0.9444608762944413 0.001429065724464869 60-61 4.330502195348088E-5 0.0 0.0 1.0626402812054905 0.0014723707464183498 62-63 4.330502195348088E-5 0.0 0.0 1.1765108364321686 0.0015156757683718308 64-65 4.330502195348088E-5 4.330502195348088E-5 0.0 1.2973968052153104 0.001580633301302052 66-67 4.330502195348088E-5 4.330502195348088E-5 0.0 1.434067454500496 0.0016239383232555331 68-69 4.330502195348088E-5 4.330502195348088E-5 0.0 1.586977487018237 0.0016672433452090138 70-71 4.330502195348088E-5 4.330502195348088E-5 0.0 1.7496311494755112 0.0016888958561857544 72-73 4.330502195348088E-5 4.330502195348088E-5 0.0 1.9155976461122268 0.001818810922046197 74-75 4.330502195348088E-5 4.330502195348088E-5 0.0 2.080524822222059 0.0019920310098601205 76-77 4.330502195348088E-5 4.330502195348088E-5 0.0 2.2618646016522597 0.0020353360318136012 78-79 4.330502195348088E-5 4.330502195348088E-5 0.0 2.468797649056968 0.0020786410537670824 80-81 4.330502195348088E-5 4.330502195348088E-5 0.0 2.682724457507164 0.0020786410537670824 82-83 4.330502195348088E-5 4.330502195348088E-5 0.0 2.904164687266288 0.002208556119627525 84-85 4.330502195348088E-5 4.330502195348088E-5 0.0 3.13160266256597 0.0022518611415810056 86-87 4.330502195348088E-5 4.330502195348088E-5 0.0 3.365817873801371 0.002360123696464708 88-89 4.330502195348088E-5 4.330502195348088E-5 0.0 3.617809796548676 0.0024683862513484103 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4760 0.0 54.362328 1 GTATCAA 8290 0.0 40.486156 1 ATCAACG 10530 0.0 31.320349 3 TATCAAC 10585 0.0 31.29229 2 TCAACGC 10630 0.0 31.025705 4 CAACGCA 10930 0.0 30.000216 5 AACGCAG 11310 0.0 29.034271 6 TAAGGTG 1455 0.0 28.088654 5 ACGCAGA 12685 0.0 25.673988 7 CGCAGAG 12740 0.0 25.5626 8 GTAAGGT 1525 0.0 24.306377 4 GTACATG 7375 0.0 23.82273 1 TGGTATC 2160 0.0 23.321022 2 TACATGG 7100 0.0 23.22558 2 GCAGAGT 14005 0.0 22.879745 9 GTGGTAT 2305 0.0 22.783869 1 GAGTACT 9335 0.0 22.58675 12-13 GGTAAGG 1610 0.0 22.432785 3 ACATGGG 7200 0.0 21.71495 3 GTACTTT 9665 0.0 21.577349 14-15 >>END_MODULE