FastQCFastQC Report
Thu 26 May 2016
SRR938001_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938001_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2309201
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103150.4466913014501553No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88440.3829896141565849No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55430.24003973668814452No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA28720.12437202305039707No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC28220.12220677195272305No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA26660.11545118852798002No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA26450.11454178306695693No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA25010.10830585990565568No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48500.052.5009161
GTATCAA85800.039.6432721
ATCAACG107600.031.2997023
TCAACGC108200.031.1666584
TATCAAC108750.031.1468012
CAACGCA109650.030.6678815
AACGCAG113500.029.7112986
TAAGGTG14900.026.77645
ACGCAGA127300.026.3411947
CGCAGAG127850.026.1164268
GTGGTAT24100.025.6253681
GTAAGGT13950.025.535614
TGGTATC23400.024.9713762
GCAGAGT143200.023.2169289
AAGGTAA14800.023.1107791
GAGTACT94350.023.03026612-13
GTACATG70650.022.5928461
TACATGG69550.022.472532
GTACTTT99650.021.66238814-15
ACATGGG69500.021.3927063
Produced by FastQC (version 0.11.5)