##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938001_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2309201 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.513463747850444 34.0 31.0 34.0 31.0 34.0 2 32.728829582180154 34.0 31.0 34.0 31.0 34.0 3 32.78796778626027 34.0 31.0 34.0 31.0 34.0 4 36.24223183689943 37.0 35.0 37.0 35.0 37.0 5 36.20616221801394 37.0 37.0 37.0 35.0 37.0 6 36.246358805491596 37.0 37.0 37.0 35.0 37.0 7 36.21332313644417 37.0 37.0 37.0 35.0 37.0 8 36.20589459297826 37.0 37.0 37.0 35.0 37.0 9 37.92053095421317 39.0 38.0 39.0 35.0 39.0 10-11 37.90661640108418 39.0 38.0 39.0 35.0 39.0 12-13 37.85753275700123 39.0 38.0 39.0 35.0 39.0 14-15 39.183450249675104 40.5 39.0 41.0 36.0 41.0 16-17 39.134513193091465 40.0 38.5 41.0 36.0 41.0 18-19 39.160701688592724 40.0 39.0 41.0 36.0 41.0 20-21 39.087760441815156 40.0 39.0 41.0 36.0 41.0 22-23 38.9440810912519 40.0 38.0 41.0 35.0 41.0 24-25 38.903142039172856 40.0 38.0 41.0 35.0 41.0 26-27 38.86680371262614 40.0 38.0 41.0 35.0 41.0 28-29 38.80763454545533 40.0 38.0 41.0 35.0 41.0 30-31 38.60701731897743 40.0 38.0 41.0 34.5 41.0 32-33 38.42827237646268 40.0 38.0 41.0 34.0 41.0 34-35 38.22913488258493 40.0 38.0 41.0 34.0 41.0 36-37 38.08677958306791 40.0 38.0 41.0 33.5 41.0 38-39 38.069810293690324 40.0 38.0 41.0 33.5 41.0 40-41 38.003619650259985 40.0 38.0 41.0 33.0 41.0 42-43 37.8536060741356 40.0 38.0 41.0 33.0 41.0 44-45 37.6422706381991 40.0 37.0 41.0 33.0 41.0 46-47 37.317219462489405 40.0 37.0 41.0 32.5 41.0 48-49 36.966491007062615 40.0 36.5 41.0 31.0 41.0 50-51 36.66243367294575 40.0 36.0 41.0 31.0 41.0 52-53 36.24656796874763 39.0 35.0 41.0 30.0 41.0 54-55 36.01892342849324 39.0 35.0 41.0 29.0 41.0 56-57 35.76695489045778 39.0 35.0 40.0 29.0 41.0 58-59 35.42621517139478 38.0 35.0 40.0 28.0 41.0 60-61 35.0634318970068 38.0 34.5 40.0 27.5 41.0 62-63 34.69281734244875 37.5 34.0 40.0 26.5 41.0 64-65 34.3013624626007 37.0 34.0 40.0 26.0 41.0 66-67 33.875358403187946 36.0 33.5 39.0 26.0 41.0 68-69 33.41631780862731 36.0 33.0 39.0 25.0 40.0 70-71 32.89732660777472 35.0 32.5 38.5 23.5 40.0 72-73 32.48410640736775 35.0 32.0 37.5 22.5 40.0 74-75 32.035506437074986 35.0 32.0 37.0 21.5 39.0 76-77 30.734156099880437 34.0 30.0 35.5 20.5 38.5 78-79 31.459732825336555 35.0 31.5 36.0 21.0 38.5 80-81 31.295591635375178 35.0 31.5 36.0 20.0 37.5 82-83 30.91904147798308 34.5 31.0 35.0 18.5 37.0 84-85 30.537026876395775 34.0 31.0 35.0 17.5 36.5 86-87 30.10235228548749 34.0 30.5 35.0 11.5 36.0 88-89 29.62275761183197 34.0 30.0 35.0 7.0 36.0 90-91 29.264503609690106 34.0 29.5 35.0 2.0 35.0 92-93 28.849801727956986 34.0 29.0 35.0 2.0 35.0 94-95 28.417937849498593 34.0 29.0 35.0 2.0 35.0 96-97 27.825335473178818 34.0 28.0 35.0 2.0 35.0 98-99 27.10991117706947 33.0 27.0 35.0 2.0 35.0 100-101 24.610849596895203 31.0 21.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11953735412214428 1101 2 0.10667962461767644 1101 3 0.08458407921578726 1101 4 0.04711042205001803 1101 5 0.08212224149485792 1101 6 0.03202427013133047 1101 7 0.10352717297848102 1101 8 0.11768984470820243 1101 9 0.09913955857765444 1101 10-11 0.11274896777496934 1101 12-13 0.18799209485692359 1101 14-15 0.22764180632934483 1101 16-17 0.21092653023865893 1101 18-19 0.24657145375039136 1101 20-21 0.17483941412121595 1101 22-23 0.25314768667483634 1101 24-25 0.2087352690640003 1101 26-27 0.23832121503970427 1101 28-29 0.28281165852510526 1101 30-31 0.27041210540098604 1101 32-33 0.25248000314644514 1101 34-35 0.21797810923354177 1101 36-37 0.2865019691505708 1101 38-39 0.43106434995099363 1101 40-41 0.5620733124808268 1101 42-43 0.4489775746042213 1101 44-45 0.47838183702284454 1101 46-47 0.5124380023404953 1101 48-49 0.46041332750998976 1101 50-51 0.44996773591085315 1101 52-53 0.45240641245830204 1101 54-55 0.5124816071257925 1101 56-57 0.592098116190293 1101 58-59 0.6298922941816656 1101 60-61 0.6102811107711048 1101 62-63 0.6275927321499211 1101 64-65 0.570746471486629 1101 66-67 0.4278575858779661 1101 68-69 0.6014431360099195 1101 70-71 0.6817366685581305 1101 72-73 0.685741260301242 1101 74-75 0.8501701645357258 1101 76-77 0.5964470955641232 1101 78-79 0.7005927396008751 1101 80-81 0.9628826515623672 1101 82-83 0.9846833801771027 1101 84-85 0.9553679515573954 1101 86-87 0.8238262391973308 1101 88-89 0.8088459236904413 1101 90-91 0.9529844252938133 1101 92-93 0.9116710507258112 1101 94-95 0.8536110656158336 1101 96-97 0.8252095302261928 1101 98-99 0.96506779934764 1101 100-101 1.0196604927520525 1102 1 0.22377600420366406 1102 2 0.17777273568717078 1102 3 0.21930529193332404 1102 4 0.10531565849629487 1102 5 0.1887113358818695 1102 6 0.16485442048316656 1102 7 0.16760978978390284 1102 8 0.15840407676696344 1102 9 0.18107988124533136 1102 10-11 0.22912103540631534 1102 12-13 0.2006025628826933 1102 14-15 0.26836397335943474 1102 16-17 0.3364416950268563 1102 18-19 0.2977895776480963 1102 20-21 0.2354785707712992 1102 22-23 0.3664320982649656 1102 24-25 0.2859598911742225 1102 26-27 0.18363138248875543 1102 28-29 0.2419906746521363 1102 30-31 0.3037481895243772 1102 32-33 0.33965317490260105 1102 34-35 0.3167116878103968 1102 36-37 0.30252225305326164 1102 38-39 0.23526385417398643 1102 40-41 0.2803559096075148 1102 42-43 0.20207469971121128 1102 44-45 0.13565561363541434 1102 46-47 0.22894052090039452 1102 48-49 0.3471660515856456 1102 50-51 0.2231168509499568 1102 52-53 0.23100039901963498 1102 54-55 0.23114614087216268 1102 56-57 0.24496269373771185 1102 58-59 0.27956754225531455 1102 60-61 0.3553415911225315 1102 62-63 0.23672133475915302 1102 64-65 0.2799828839062286 1102 66-67 0.1893584804137376 1102 68-69 0.34296091616467095 1102 70-71 0.4482371240254821 1102 72-73 0.3881350824499705 1102 74-75 0.4921097677659958 1102 76-77 0.4015938237638501 1102 78-79 0.45679413128904756 1102 80-81 0.3994695347373778 1102 82-83 0.4876089512040913 1102 84-85 0.4104821463746937 1102 86-87 0.4118599850433746 1102 88-89 0.3257884628601069 1102 90-91 0.3861588476014539 1102 92-93 0.5233191654295233 1102 94-95 0.35359519567319 1102 96-97 0.49862346170824523 1102 98-99 0.3940493828081948 1102 100-101 0.6595052635530507 1103 1 0.1462228240900174 1103 2 0.13634589729055335 1103 3 0.1444684243209906 1103 4 0.08115461189248663 1103 5 0.050217500436325224 1103 6 0.07649928904928771 1103 7 0.10384466109091761 1103 8 0.11285852199393531 1103 9 0.17171905047160152 1103 10-11 0.13951375765537932 1103 12-13 0.16468486100598057 1103 14-15 0.2504353890253057 1103 16-17 0.3063075263967008 1103 18-19 0.23231784266186395 1103 20-21 0.3297573529291853 1103 22-23 0.279844413607961 1103 24-25 0.28345160396288804 1103 26-27 0.24740204213479444 1103 28-29 0.3302007722058633 1103 30-31 0.19931488482908577 1103 32-33 0.2119910964687719 1103 34-35 0.24242146272518283 1103 36-37 0.2891017027357279 1103 38-39 0.2773519487360758 1103 40-41 0.3964499997227193 1103 42-43 0.29409132206880173 1103 44-45 0.3649673910010378 1103 46-47 0.37222416169326067 1103 48-49 0.4968376358841269 1103 50-51 0.4974760900383117 1103 52-53 0.45489399331562197 1103 54-55 0.5553331215003183 1103 56-57 0.5835209340588463 1103 58-59 0.6188432700980115 1103 60-61 0.5592177444798807 1103 62-63 0.710644120196271 1103 64-65 0.7024346201953051 1103 66-67 0.7450594795496812 1103 68-69 0.7508324456452584 1103 70-71 0.7479567047054942 1103 72-73 0.5647350335317611 1103 74-75 0.4961946838737319 1103 76-77 0.5080038296266594 1103 78-79 0.5184903762635606 1103 80-81 0.5584918097289879 1103 82-83 0.6767380473357392 1103 84-85 0.8431729058728763 1103 86-87 0.924444746898974 1103 88-89 0.790498216880188 1103 90-91 0.7032806502496705 1103 92-93 0.782615468417589 1103 94-95 0.755683806457565 1103 96-97 0.9424787572270681 1103 98-99 1.0345365122804289 1103 100-101 0.9427908463249892 1104 1 0.13890197245211766 1104 2 0.09945263497525048 1104 3 0.10626262602671943 1104 4 0.037091741477716766 1104 5 0.028345808888730062 1104 6 0.04178479346993669 1104 7 0.06949381438112567 1104 8 0.10847793072159106 1104 9 0.025218384335651933 1104 10-11 0.08407938582216445 1104 12-13 0.10517902875030671 1104 14-15 0.12613010296593785 1104 16-17 0.12585161115249122 1104 18-19 0.134432008475585 1104 20-21 0.1912122141583552 1104 22-23 0.24180764071518013 1104 24-25 0.15997170899702695 1104 26-27 0.23797081554188537 1104 28-29 0.12394464381377901 1104 30-31 0.11345906720925569 1104 32-33 0.11082333611982875 1104 34-35 0.19030331064765704 1104 36-37 0.30031893183686265 1104 38-39 0.052258574414572934 1104 40-41 0.20081667815276916 1104 42-43 0.1602828026414329 1104 44-45 0.16706570946208643 1104 46-47 0.12120111835280056 1104 48-49 0.1162409898952319 1104 50-51 0.2545430127638042 1104 52-53 0.21387881698402822 1104 54-55 0.19305794970993162 1104 56-57 0.24826911356733916 1104 58-59 0.27566787097334355 1104 60-61 0.20826580905109893 1104 62-63 0.1421864877321326 1104 64-65 0.07422836465313054 1104 66-67 0.13774163715013543 1104 68-69 0.22583798537951338 1104 70-71 0.27138333224424827 1104 72-73 0.21440761276559783 1104 74-75 0.15739059427971114 1104 76-77 0.12430698420732611 1104 78-79 0.25579236740233213 1104 80-81 0.194317886694364 1104 82-83 0.3116318466242305 1104 84-85 0.4683990384114587 1104 86-87 0.4301808632697366 1104 88-89 0.37643043788959574 1104 90-91 0.2887026685731975 1104 92-93 0.5138159356702374 1104 94-95 0.5819253029166269 1104 96-97 0.7746238322176815 1104 98-99 0.7621643883013753 1104 100-101 0.9060740348045329 1105 1 0.11092699720848742 1105 2 0.09872780549341797 1105 3 0.08109590228929164 1105 4 0.015079025495744247 1105 5 0.0835133655507363 1105 6 0.07928990670140479 1105 7 0.035452927038804205 1105 8 0.050778653515031635 1105 9 -0.015624720437003248 1105 10-11 0.0667273456196682 1105 12-13 0.11946664686364272 1105 14-15 0.13376413441346102 1105 16-17 0.10559257160708313 1105 18-19 0.10337058491492002 1105 20-21 0.12165829154630359 1105 22-23 0.02404219227783244 1105 24-25 0.11326999779974756 1105 26-27 0.10863715520852679 1105 28-29 0.054032471083722555 1105 30-31 0.14445663321853175 1105 32-33 0.20729858437762516 1105 34-35 0.29467949640834945 1105 36-37 0.2957106377589511 1105 38-39 0.29975813481548386 1105 40-41 0.2463429476686585 1105 42-43 0.22773109190688245 1105 44-45 0.2691351568629017 1105 46-47 0.32739382008251283 1105 48-49 0.46592845808213923 1105 50-51 0.4377798502417036 1105 52-53 0.38758042637941514 1105 54-55 0.4309206652291593 1105 56-57 0.44602021719241947 1105 58-59 0.46596032004925547 1105 60-61 0.4975536585323681 1105 62-63 0.521788812517201 1105 64-65 0.565363640860177 1105 66-67 0.4404996355398154 1105 68-69 0.3739958338427698 1105 70-71 0.5247761117135852 1105 72-73 0.4741699516178244 1105 74-75 0.5956443216172289 1105 76-77 0.502907578360194 1105 78-79 0.6722129202791614 1105 80-81 0.5365929739200759 1105 82-83 0.616979870279291 1105 84-85 0.6314122819123291 1105 86-87 0.49932804104360784 1105 88-89 0.29285737652156385 1105 90-91 0.374048748737561 1105 92-93 0.27600634222998366 1105 94-95 0.23179934621019882 1105 96-97 0.4133099399610991 1105 98-99 0.438752451566625 1105 100-101 0.6200302414808831 1106 1 -0.11869682100948609 1106 2 -0.030561227207819286 1106 3 -0.017679925184083345 1106 4 -0.07755786237279949 1106 5 -0.0720472917279551 1106 6 -0.030869712959109563 1106 7 -0.018939596325679986 1106 8 -0.10430657940674593 1106 9 -0.08982960483476887 1106 10-11 -0.061544676651166697 1106 12-13 -0.17937480001717887 1106 14-15 -0.2525270132934665 1106 16-17 -0.22555676225650956 1106 18-19 -0.12544428881742675 1106 20-21 -0.035051338056547365 1106 22-23 0.007928018124289338 1106 24-25 -0.08091319423191834 1106 26-27 -0.06551241365569638 1106 28-29 0.00602563798840805 1106 30-31 -0.02509162325754488 1106 32-33 0.044323476367715386 1106 34-35 0.0012643949061512672 1106 36-37 -0.018076307052048435 1106 38-39 0.0333738907040555 1106 40-41 0.0011920953394408684 1106 42-43 0.008985814515760637 1106 44-45 -0.012620297863321639 1106 46-47 -0.02507133655445415 1106 48-49 0.09343546290868687 1106 50-51 -0.023574657251195674 1106 52-53 -0.17874922527733617 1106 54-55 -0.42525334370085943 1106 56-57 -0.4704921926486847 1106 58-59 -0.24737343508007825 1106 60-61 -0.14724125822317546 1106 62-63 -0.17573211157760227 1106 64-65 -0.3127247193777194 1106 66-67 -0.1656470771628733 1106 68-69 -0.2633385359036069 1106 70-71 -0.5276028177681482 1106 72-73 -0.45102392390414536 1106 74-75 -0.4521826456092235 1106 76-77 -0.24200628486943643 1106 78-79 -0.2721962338993329 1106 80-81 -0.5156413397876918 1106 82-83 -0.4249805773065276 1106 84-85 -0.2752583014315455 1106 86-87 -0.08142414175073398 1106 88-89 -0.19198309420277937 1106 90-91 -0.34948114153521814 1106 92-93 -0.06221976453261391 1106 94-95 -0.20226164549247372 1106 96-97 0.011393780956058919 1106 98-99 0.2055774122492089 1106 100-101 -0.03512043007861365 1107 1 0.09670687894737284 1107 2 0.0869079369331871 1107 3 0.08171703322619095 1107 4 0.0494115091524705 1107 5 0.0521627265592528 1107 6 0.044159767458488375 1107 7 0.04317908840799589 1107 8 0.05329022819120155 1107 9 0.03383096632711613 1107 10-11 0.016295050972630065 1107 12-13 0.10019528790128618 1107 14-15 0.1137708971456064 1107 16-17 0.10613705824635389 1107 18-19 0.09485977321366335 1107 20-21 0.09302904533083023 1107 22-23 0.09076920332363159 1107 24-25 -0.008689287084678199 1107 26-27 0.12874709295671494 1107 28-29 0.07676669571047512 1107 30-31 0.0900428233726771 1107 32-33 0.02383138362852577 1107 34-35 0.06151817923620939 1107 36-37 0.013511709391892168 1107 38-39 0.013579945493667367 1107 40-41 0.1164681449150109 1107 42-43 0.1431402372015853 1107 44-45 0.11935485895588016 1107 46-47 0.0518121384164445 1107 48-49 0.10017406664132977 1107 50-51 0.09436449710570827 1107 52-53 0.07517618840061147 1107 54-55 0.08667696410724801 1107 56-57 0.11911494746345141 1107 58-59 0.22089954216166063 1107 60-61 0.2909407469160783 1107 62-63 0.31332481973078075 1107 64-65 0.31229925618210075 1107 66-67 0.2601396501056996 1107 68-69 0.2648219275846415 1107 70-71 0.09331726900779103 1107 72-73 0.18217078395409203 1107 74-75 0.042383644303590984 1107 76-77 0.19006809787674328 1107 78-79 0.2797443496432912 1107 80-81 0.30006121508950656 1107 82-83 0.389536009626255 1107 84-85 0.527351001154905 1107 86-87 0.4859390452226293 1107 88-89 0.46332348482931707 1107 90-91 0.36674499802227345 1107 92-93 0.5081617463465804 1107 94-95 0.6408177096290153 1107 96-97 0.8031320280847041 1107 98-99 0.9032002484765762 1107 100-101 0.6382284068730364 1108 1 0.025019097411501434 1108 2 0.03346455345653965 1108 3 0.036746318609402806 1108 4 0.049657652310159506 1108 5 0.004225447330135523 1108 6 0.0260998483003263 1108 7 0.04237180051505618 1108 8 0.060546569589192245 1108 9 0.016881517656955225 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-0.4888643423651331 2316 66-67 -0.4336745681087635 2316 68-69 -0.35235129182947844 2316 70-71 -0.41435846701306644 2316 72-73 -0.24337127533523528 2316 74-75 -0.4047005003748083 2316 76-77 -0.32481210269365945 2316 78-79 -0.19630477506724375 2316 80-81 -0.45525451271614514 2316 82-83 -0.45479347678960025 2316 84-85 -0.20056417836946494 2316 86-87 -0.25107648195669086 2316 88-89 -0.2446330721282699 2316 90-91 -0.24243959902080903 2316 92-93 -0.2945999961352186 2316 94-95 -0.16210303939666915 2316 96-97 -0.23853949344712788 2316 98-99 -0.16490496522915166 2316 100-101 -0.08701682984397507 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 8.0 4 12.0 5 40.0 6 81.0 7 197.0 8 472.0 9 939.0 10 1555.0 11 2664.0 12 4022.0 13 5957.0 14 8023.0 15 10996.0 16 14130.0 17 19806.0 18 19943.0 19 14597.0 20 12256.0 21 11866.0 22 12349.0 23 13170.0 24 15097.0 25 17561.0 26 20472.0 27 25224.0 28 30803.0 29 37302.0 30 46277.0 31 56667.0 32 71253.0 33 93117.0 34 130120.0 35 200222.0 36 332586.0 37 546964.0 38 480022.0 39 52422.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.845642280598355 18.925680354373657 15.338335640769252 22.890341724258736 2 15.661829768858857 19.906065749644085 40.191974442676845 24.240130038820208 3 19.44231396354474 23.547980426811232 29.985271171121617 27.024434438522405 4 13.071534266614297 17.015149395829987 36.751153320997176 33.162163016558544 5 13.781476796519662 37.044891284907635 34.36976685875331 14.803865059819394 6 32.4954822035847 36.20065988192453 16.938932557191862 14.36492535729891 7 28.567846627469844 32.715558325152294 20.191053095854368 18.52554195152349 8 26.361212060322398 35.07278736458467 19.25738526722767 19.308615307865264 9 25.89942793793495 16.97911388841975 20.182964589314867 36.93849358433043 10-11 25.011833097248786 26.630271682716227 26.885901227307627 21.471993992727352 12-13 26.04268749234042 23.681784305480555 27.501828554551988 22.773699647627037 14-15 22.609162216714786 25.326487386762782 25.958459224640904 26.105891171881527 16-17 22.0212043820513 28.477140818706104 26.904837946598214 22.596816852644377 18-19 22.026406536286792 26.732666407125233 29.446830310570625 21.794096746017345 20-21 22.440510808716954 25.882588826178406 29.919980980434357 21.756919384670283 22-23 22.24964392445699 25.838872406516366 29.842508296159583 22.068975372867065 24-25 22.160027803431472 25.995363929378417 29.855818420206425 21.988789846983686 26-27 21.692616126095942 26.34235960577584 29.64241788566789 22.32260638246033 28-29 21.823462276229368 26.78238637138698 29.502007296464726 21.892144055918923 30-31 21.92000609734709 26.274585884901313 29.43977159199221 22.365636425759387 32-33 21.646635094240917 26.301799593623386 29.453210392570895 22.5983549195648 34-35 22.433957356661352 26.459850971595287 29.163018415340392 21.943173256402964 36-37 22.500499109830578 25.963152052889622 29.28609755009612 22.25025128718368 38-39 21.946112290785294 26.40558389540808 29.16067234570602 22.48763146810061 40-41 22.434356119446104 26.22054230251072 29.244852556943624 22.100249021099554 42-43 22.12463293036607 26.271824145250978 29.030620011767017 22.572922912615937 44-45 22.152727449102507 26.52383920932872 29.150243609434128 22.17318973213464 46-47 22.63032652000693 26.778169928979732 28.36413043478261 22.227373116230726 48-49 22.325990678160974 26.818626875702893 28.332094087955095 22.523288358181034 50-51 22.48165057957276 27.069254690258663 28.290672834456593 22.15842189571198 52-53 22.831728377044698 26.731973526773977 28.192738527308798 22.243559568872524 54-55 22.37126607861334 26.952807486225755 28.320098596873983 22.355827838286924 56-57 22.372760101870735 27.107384762088703 28.13453657780332 22.385318558237245 58-59 22.348769119708507 26.766769111913604 28.120116005492807 22.764345762885085 60-61 22.09469855590743 26.982687951373656 28.402529706162433 22.520083786556473 62-63 22.184404908884066 26.99143556580826 28.495050885566048 22.329108639741623 64-65 21.955970918079455 27.11113064648768 28.2503558590179 22.68254257641496 66-67 21.84939725905194 27.15995705874023 28.19724224959196 22.793403432615868 68-69 22.34818245063376 26.788756858722813 28.381433439236066 22.481627251407364 70-71 22.26079496761001 26.91409279659934 27.935311824306332 22.889800411484316 72-73 22.229940139468155 27.011529095994675 27.942998465703074 22.8155322988341 74-75 22.20005967432025 27.466751486769663 27.72458958748069 22.60859925142939 76-77 22.340346292938555 27.04225400907067 27.95850166356242 22.65889803442836 78-79 22.309794599950372 27.21976129405799 27.970886899841112 22.499557206150524 80-81 22.50808829547536 27.259775134343005 27.656708965568612 22.575427604613026 82-83 22.476468577097823 27.338001762082488 27.744917665190904 22.440611995628785 84-85 22.39398266097967 27.489068379521786 27.709757843548665 22.407191115949882 86-87 22.54124867296857 27.45293761398721 27.45661854962042 22.5491951634238 88-89 22.43574503539972 27.585820884211365 27.26575318974242 22.712680890646496 90-91 22.309794770605272 27.595659355970785 27.44770746014902 22.64683841327492 92-93 22.422441452439404 27.24434661316452 27.637601220514025 22.695610713882047 94-95 22.217381683101646 27.073845022585736 27.746631843655013 22.962141450657608 96-97 22.454156752521058 26.73927621269786 27.782537722471478 23.024029312309608 98-99 22.146642929740633 27.104396715573913 27.439859068136556 23.3091012865489 100-101 22.311375233251674 26.778981128104483 27.768804014895192 23.140839623748647 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 917.0 1 610.5 2 431.0 3 588.5 4 479.0 5 411.0 6 829.5 7 2025.5 8 3357.5 9 3863.5 10 3234.0 11 2369.5 12 2236.5 13 2339.0 14 2481.5 15 2627.0 16 2808.0 17 3134.5 18 3395.5 19 3666.5 20 4088.5 21 4864.5 22 5928.0 23 7305.5 24 9165.0 25 11582.0 26 14476.5 27 17887.0 28 21274.5 29 24622.0 30 28182.5 31 32781.0 32 39678.0 33 43126.5 34 43249.5 35 45573.0 36 50046.5 37 54499.0 38 57829.5 39 60965.0 40 64627.0 41 69173.0 42 71535.0 43 73735.5 44 77467.5 45 82141.5 46 84179.5 47 85703.5 48 86977.5 49 84320.0 50 82334.5 51 80990.0 52 79639.0 53 80324.5 54 81702.5 55 77030.5 56 70324.0 57 64507.5 58 59493.0 59 53266.5 60 42576.5 61 34569.5 62 28876.5 63 20818.5 64 14025.0 65 10385.0 66 7704.5 67 5962.5 68 4645.5 69 3459.0 70 2519.5 71 1685.0 72 1176.5 73 924.5 74 665.5 75 455.0 76 315.0 77 195.5 78 112.5 79 67.5 80 43.5 81 26.5 82 18.5 83 12.0 84 9.0 85 4.5 86 3.0 87 2.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.015243367727625272 3 0.005369822722231629 4 0.0 5 0.0 6 0.0 7 0.0 8 3.897451975813279E-4 9 4.7635524148828974E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.680926866045875E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.005781220430789697 26-27 0.022280433795065915 28-29 2.165251097674044E-5 30-31 0.0 32-33 5.629652853952514E-4 34-35 0.019335692302229212 36-37 0.003897451975813279 38-39 0.018123151687531747 40-41 0.015611460414229858 42-43 0.00885587698948684 44-45 0.0032262241355343253 46-47 4.330502195348088E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.814826426976257E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 6.495753293022131E-5 84-85 0.003204571624557585 86-87 2.3817762074414487E-4 88-89 8.661004390696177E-5 90-91 1.9487259879066396E-4 92-93 4.330502195348088E-4 94-95 0.0 96-97 2.165251097674044E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2309201.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.550016571733664 #Duplication Level Percentage of deduplicated Percentage of total 1 75.89833719227111 42.161538887976896 2 13.829017236179714 15.364042732811473 3 4.560661365886458 7.600344433591746 4 1.9689125255603752 4.374924936926913 5 1.0327051996725416 2.8683395477762605 6 0.6173968565628166 2.057784336804044 7 0.4172586723541251 1.6225108314779864 8 0.280802030857286 1.2478845973998964 9 0.20233574117949948 1.011577839801769 >10 1.0812202693809565 10.786725997843842 >50 0.07003945906768966 2.6666464621676114 >100 0.035149707506679784 3.6154193392213227 >500 0.003432971074851799 1.302188539762068 >1k 0.0024967062362552662 2.249455396977165 >5k 1.5604413976595414E-4 0.6235508910485439 >10k+ 7.802206988297707E-5 0.44706522841215895 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10315 0.4466913014501553 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8844 0.3829896141565849 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5543 0.24003973668814452 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2872 0.12437202305039707 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2822 0.12220677195272305 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2666 0.11545118852798002 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2645 0.11454178306695693 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2501 0.10830585990565568 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 8.661004390696177E-5 7 0.0 0.0 0.0 4.330502195348088E-5 8.661004390696177E-5 8 0.0 0.0 0.0 4.330502195348088E-5 8.661004390696177E-5 9 0.0 0.0 0.0 4.330502195348088E-5 8.661004390696177E-5 10-11 0.0 0.0 0.0 4.330502195348088E-5 8.661004390696177E-5 12-13 0.0 0.0 0.0 4.330502195348088E-5 4.980077524650301E-4 14-15 0.0 0.0 0.0 4.330502195348088E-5 5.846177963719918E-4 16-17 0.0 0.0 0.0 6.495753293022132E-5 6.495753293022132E-4 18-19 0.0 0.0 0.0 8.661004390696177E-5 6.495753293022132E-4 20-21 0.0 0.0 0.0 1.082625548837022E-4 6.928803512556941E-4 22-23 0.0 2.165251097674044E-5 0.0 2.598301317208853E-4 6.928803512556941E-4 24-25 0.0 4.330502195348088E-5 0.0 3.247876646511066E-4 7.361853732091749E-4 26-27 0.0 4.330502195348088E-5 0.0 8.444479280928772E-4 8.227954171161366E-4 28-29 0.0 4.330502195348088E-5 0.0 0.0031396140916273635 8.661004390696175E-4 30-31 0.0 4.330502195348088E-5 0.0 0.009548757340742535 8.87752950046358E-4 32-33 0.0 4.330502195348088E-5 0.0 0.023298101810972714 9.527104829765794E-4 34-35 0.0 4.330502195348088E-5 0.0 0.04423607992548072 9.527104829765794E-4 36-37 0.0 4.330502195348088E-5 0.0 0.07978950294928852 9.527104829765794E-4 38-39 0.0 4.330502195348088E-5 0.0 0.13099769140927967 9.960155049300603E-4 40-41 0.0 4.330502195348088E-5 0.0 0.18517227387308424 9.960155049300603E-4 42-43 0.0 4.330502195348088E-5 0.0 0.2412089722808885 9.960155049300603E-4 44-45 0.0 4.330502195348088E-5 0.0 0.3090462891710163 0.0010393205268835412 46-47 4.330502195348088E-5 4.330502195348088E-5 0.0 0.38948536744960705 0.0011042780598137624 48-49 4.330502195348088E-5 4.330502195348088E-5 0.0 0.4706822836123837 0.0011692355927439837 50-51 4.330502195348088E-5 4.330502195348088E-5 0.0 0.5564045745692991 0.0011692355927439837 52-53 6.495753293022132E-5 4.330502195348088E-5 0.0 0.641412332663982 0.0012558456366509455 54-55 8.661004390696177E-5 4.330502195348088E-5 0.0 0.7333921992931755 0.0012991506586044264 56-57 8.661004390696177E-5 4.330502195348088E-5 0.0 0.8385151400852503 0.0013424556805579073 58-59 8.661004390696177E-5 4.330502195348088E-5 0.0 0.9495059113520217 0.0013857607025113882 60-61 8.661004390696177E-5 4.330502195348088E-5 0.0 1.0685081116801873 0.001429065724464869 62-63 8.661004390696177E-5 4.330502195348088E-5 0.0 1.1823786669068652 0.0015589807903253117 64-65 8.661004390696177E-5 4.330502195348088E-5 0.0 1.3041307361290766 0.0016022858122787926 66-67 8.661004390696177E-5 4.330502195348088E-5 0.0 1.4427717639131457 0.0016022858122787926 68-69 8.661004390696177E-5 4.330502195348088E-5 0.0 1.5974789548419563 0.0016022858122787926 70-71 8.661004390696177E-5 4.330502195348088E-5 0.0 1.7606089725407186 0.0016022858122787926 72-73 8.661004390696177E-5 4.330502195348088E-5 0.0 1.9275498321713873 0.0017105483671624947 74-75 8.661004390696177E-5 4.330502195348088E-5 0.0 2.094512344313033 0.0018837684549764182 76-77 8.661004390696177E-5 4.330502195348088E-5 0.0 2.2779307647970013 0.0019487259879066396 78-79 8.661004390696177E-5 4.330502195348088E-5 0.0 2.486660970612779 0.0019920310098601205 80-81 8.661004390696177E-5 4.330502195348088E-5 0.0 2.7033159954460437 0.0019920310098601205 82-83 8.661004390696177E-5 4.330502195348088E-5 0.0 2.929454820087121 0.0020786410537670824 84-85 8.661004390696177E-5 4.330502195348088E-5 0.0 3.160617027274802 0.0020786410537670824 86-87 8.661004390696177E-5 4.330502195348088E-5 0.0 3.398859605551877 0.002165251097674044 88-89 8.661004390696177E-5 4.330502195348088E-5 0.0 3.655463513137228 0.0022518611415810056 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4850 0.0 52.500916 1 GTATCAA 8580 0.0 39.643272 1 ATCAACG 10760 0.0 31.299702 3 TCAACGC 10820 0.0 31.166658 4 TATCAAC 10875 0.0 31.146801 2 CAACGCA 10965 0.0 30.667881 5 AACGCAG 11350 0.0 29.711298 6 TAAGGTG 1490 0.0 26.7764 5 ACGCAGA 12730 0.0 26.341194 7 CGCAGAG 12785 0.0 26.116426 8 GTGGTAT 2410 0.0 25.625368 1 GTAAGGT 1395 0.0 25.53561 4 TGGTATC 2340 0.0 24.971376 2 GCAGAGT 14320 0.0 23.216928 9 AAGGTAA 1480 0.0 23.110779 1 GAGTACT 9435 0.0 23.030266 12-13 GTACATG 7065 0.0 22.592846 1 TACATGG 6955 0.0 22.47253 2 GTACTTT 9965 0.0 21.662388 14-15 ACATGGG 6950 0.0 21.392706 3 >>END_MODULE