FastQCFastQC Report
Thu 26 May 2016
SRR938000_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938000_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2444478
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107050.43792580665483594No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87580.3582769000170998No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54750.22397419817237052No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA29880.12223468568749647No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA27730.11343935187798784No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC27680.1132348092312551No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA27230.11139392541066027No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA25140.10284404277723096No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51700.056.461511
GTATCAA90750.041.925931
ATCAACG117350.031.9406913
TCAACGC118550.031.6976244
TATCAAC119800.031.2868372
CAACGCA119650.031.2471965
AACGCAG124250.030.1243136
TGGTATC21700.028.9338652
GTGGTAT22750.028.0482651
ACGCAGA139550.026.6456727
CGCAGAG139950.026.3953028
TAAGGTG16400.026.1035675
GTAAGGT15100.025.5158024
GCAGAGT151200.023.9602099
AGGTAAG16000.023.1881922
GAGTACT103000.022.7297212-13
GTACATG76200.022.37231
GGTAAGG17300.021.9960673
CAGAGTA145250.021.71650510-11
AAGGTAA16300.021.6185571
Produced by FastQC (version 0.11.5)