##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938000_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2444478 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.92108621963462 33.0 31.0 34.0 30.0 34.0 2 32.04701044558389 33.0 31.0 34.0 30.0 34.0 3 32.06808365630617 34.0 31.0 34.0 30.0 34.0 4 35.587345028263705 37.0 35.0 37.0 33.0 37.0 5 35.58922191159012 37.0 35.0 37.0 33.0 37.0 6 35.61716898249851 37.0 35.0 37.0 33.0 37.0 7 35.54565228240958 37.0 35.0 37.0 33.0 37.0 8 35.537344987355176 37.0 35.0 37.0 33.0 37.0 9 37.16510027907799 39.0 37.0 39.0 34.0 39.0 10-11 37.06514990112409 39.0 37.0 39.0 33.0 39.0 12-13 36.90973982993506 39.0 37.0 39.0 33.0 39.0 14-15 38.07248439135063 40.0 38.0 41.0 33.0 41.0 16-17 37.883938002305605 40.0 38.0 41.0 33.0 41.0 18-19 38.07066355270941 40.0 38.0 41.0 33.0 41.0 20-21 38.06319467796396 40.0 38.0 41.0 33.0 41.0 22-23 37.94246194893143 40.0 38.0 41.0 33.0 41.0 24-25 37.81205762539078 40.0 38.0 41.0 32.5 41.0 26-27 37.61598795325628 40.0 38.0 41.0 32.0 41.0 28-29 37.421981298256725 40.0 37.0 41.0 32.0 41.0 30-31 37.16947483266366 40.0 37.0 41.0 31.0 41.0 32-33 36.85385018805651 39.5 36.0 41.0 30.5 41.0 34-35 36.60374709856255 39.0 36.0 41.0 30.0 41.0 36-37 36.30953847815361 39.0 35.0 41.0 30.0 41.0 38-39 35.98179979529372 39.0 35.0 40.0 29.0 41.0 40-41 35.833003201501505 39.0 35.0 40.0 28.0 41.0 42-43 35.58820860731821 39.0 35.0 40.0 27.0 41.0 44-45 35.22645570956253 38.5 35.0 40.0 25.5 41.0 46-47 34.81183303756467 38.0 34.0 40.0 24.0 41.0 48-49 34.37060795801803 38.0 33.5 40.0 23.0 41.0 50-51 33.62566916126879 37.5 33.0 39.5 21.5 40.5 52-53 33.68858627486114 38.0 33.0 39.5 21.5 40.5 54-55 33.743265842441616 38.0 33.0 40.0 19.5 41.0 56-57 33.39250322563754 38.0 33.0 40.0 17.5 41.0 58-59 32.95045649827898 37.0 32.5 40.0 13.0 41.0 60-61 32.414688330187474 36.5 31.5 39.5 8.5 41.0 62-63 31.83306681426464 36.0 31.0 39.0 6.5 41.0 64-65 31.254522437919263 35.0 30.5 39.0 2.0 40.0 66-67 30.638010446402056 35.0 29.5 38.5 2.0 40.0 68-69 29.916570940708 35.0 28.5 38.0 2.0 40.0 70-71 29.287273601971464 34.0 28.0 37.0 2.0 39.5 72-73 28.549373526781586 34.0 26.0 36.5 2.0 39.0 74-75 27.835690278251633 34.0 26.0 36.0 2.0 39.0 76-77 27.048615491732797 33.0 24.5 35.0 2.0 38.0 78-79 26.238358455261206 32.0 21.5 35.0 2.0 37.0 80-81 25.471579208321778 32.0 19.0 35.0 2.0 36.5 82-83 24.688341437312996 31.0 15.0 35.0 2.0 36.0 84-85 23.91632733041574 31.0 7.5 34.0 2.0 35.5 86-87 23.12303649286269 30.0 2.0 34.0 2.0 35.0 88-89 22.189260651967416 29.5 2.0 34.0 2.0 35.0 90-91 21.2398268669221 29.0 2.0 34.0 2.0 35.0 92-93 20.259665662771354 27.5 2.0 34.0 2.0 35.0 94-95 19.1342010441493 25.5 2.0 33.0 2.0 35.0 96-97 17.82752309491024 24.0 2.0 32.5 2.0 34.5 98-99 16.24674777191695 10.0 2.0 32.0 2.0 34.0 100-101 12.517790096699581 2.0 2.0 27.5 2.0 32.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2389077344526207 1101 2 0.2180372651393938 1101 3 0.23278679930619717 1101 4 0.1015666854855013 1101 5 0.26650407835398937 1101 6 0.2184780352689515 1101 7 0.272587524150687 1101 8 0.3198839922178749 1101 9 0.3386030141373553 1101 10-11 0.3086885801209931 1101 12-13 0.3072749763900475 1101 14-15 0.34940346194096605 1101 16-17 0.3656828003866366 1101 18-19 0.41034913485586344 1101 20-21 0.42351684876076234 1101 22-23 0.48162351996091957 1101 24-25 0.3888861036546629 1101 26-27 0.4656173200662437 1101 28-29 0.4995762887485071 1101 30-31 0.4652723737817581 1101 32-33 0.3631156701404379 1101 34-35 0.31413652978579165 1101 36-37 0.2064386220462353 1101 38-39 0.2063322836618866 1101 40-41 0.2694495565982322 1101 42-43 0.28587600425699833 1101 44-45 0.21777038979683994 1101 46-47 0.22377011759502352 1101 48-49 0.20413157565655382 1101 50-51 0.297385193491877 1101 52-53 0.24643065874719383 1101 54-55 0.1456982615214244 1101 56-57 0.24630429373328155 1101 58-59 0.2816995748345832 1101 60-61 0.1361098808103094 1101 62-63 0.032951314544082067 1101 64-65 -0.13639306180025557 1101 66-67 -0.040512025142216856 1101 68-69 -0.01989108421300756 1101 70-71 -0.030988052251188947 1101 72-73 -0.2608934623481076 1101 74-75 -0.10347805515285202 1101 76-77 -0.11377675946773635 1101 78-79 -0.3309313493042474 1101 80-81 -0.3804590406731876 1101 82-83 -0.4837757165283243 1101 84-85 -0.4573033156798232 1101 86-87 -0.27494568684182497 1101 88-89 -0.0021749976244862523 1101 90-91 -0.0335631116323718 1101 92-93 -0.1853903079599597 1101 94-95 -0.4305732097882 1101 96-97 -0.38523788317156615 1101 98-99 -0.6112591539091312 1101 100-101 -1.0146669104059765 1102 1 0.3270095057460658 1102 2 0.29837382576641147 1102 3 0.3135955561288739 1102 4 0.24519984481267443 1102 5 0.23525361359395447 1102 6 0.26894539796024475 1102 7 0.34928780226309186 1102 8 0.29552870733856906 1102 9 0.2559854112580737 1102 10-11 0.38465453737794064 1102 12-13 0.3727085417315905 1102 14-15 0.4529269007190564 1102 16-17 0.44463344658899473 1102 18-19 0.38137971234809953 1102 20-21 0.32868349311895173 1102 22-23 0.43588555538678264 1102 24-25 0.4331266113957071 1102 26-27 0.44309876684564387 1102 28-29 0.33097714985590443 1102 30-31 0.36896851738889325 1102 32-33 0.3600324520675926 1102 34-35 0.341111202222919 1102 36-37 0.23767328496507645 1102 38-39 0.2594199634310712 1102 40-41 0.44724257850516125 1102 42-43 0.6037951062662543 1102 44-45 0.5531458787244006 1102 46-47 0.538659913695497 1102 48-49 0.35220691139920035 1102 50-51 0.38873935551094263 1102 52-53 0.4864513312735568 1102 54-55 0.3463249439527516 1102 56-57 0.3639081025347224 1102 58-59 0.32408104243522473 1102 60-61 0.42864750778274896 1102 62-63 0.37940947126859825 1102 64-65 0.3141973693483351 1102 66-67 0.19229092403906378 1102 68-69 0.2454320541441959 1102 70-71 -0.03422464113561574 1102 72-73 0.0021951097603434278 1102 74-75 -0.0522159528015429 1102 76-77 -0.15491174065424929 1102 78-79 0.049153125479730164 1102 80-81 0.148551280717669 1102 82-83 0.0993038578258485 1102 84-85 0.09361579376558637 1102 86-87 0.003568270831550535 1102 88-89 0.017909142532268163 1102 90-91 0.008479081142652944 1102 92-93 -0.3959154496188191 1102 94-95 -0.43883385465204583 1102 96-97 -0.8851486492448295 1102 98-99 -0.6679998475541886 1102 100-101 -1.1290610864050237 1103 1 0.3804138156324335 1103 2 0.4078509499494132 1103 3 0.3631768715630912 1103 4 0.3303473205900218 1103 5 0.41895009009821393 1103 6 0.35135081238087196 1103 7 0.41422009840415086 1103 8 0.3586094925334038 1103 9 0.4111264517859894 1103 10-11 0.45310379960570657 1103 12-13 0.3976020611679303 1103 14-15 0.5677097621865457 1103 16-17 0.5347342156721027 1103 18-19 0.5197537026118297 1103 20-21 0.4811745168113717 1103 22-23 0.4822401989935443 1103 24-25 0.4635003200189445 1103 26-27 0.5885043291668666 1103 28-29 0.5466124851969099 1103 30-31 0.4900987139708093 1103 32-33 0.4817096055160377 1103 34-35 0.43850846637653973 1103 36-37 0.4185051707606391 1103 38-39 0.5353043382048881 1103 40-41 0.7531682789088947 1103 42-43 0.7507951235737664 1103 44-45 0.7575423420120089 1103 46-47 0.6879785482268232 1103 48-49 0.6888992797283322 1103 50-51 0.7424210183542215 1103 52-53 0.5902657506378404 1103 54-55 0.5789456402813542 1103 56-57 0.4517274262199038 1103 58-59 0.7069640263140613 1103 60-61 0.7219404910007512 1103 62-63 0.37580714765579515 1103 64-65 0.2587826340432038 1103 66-67 0.3497576621385754 1103 68-69 0.397217599850201 1103 70-71 0.27921566838572076 1103 72-73 0.14827074832903975 1103 74-75 0.16231799510597966 1103 76-77 0.21067139678946134 1103 78-79 0.0538812243906186 1103 80-81 0.24977468614467924 1103 82-83 0.19675206114825983 1103 84-85 -0.047364157836305765 1103 86-87 -0.061129587127233265 1103 88-89 0.07798255000471244 1103 90-91 0.1335380840408753 1103 92-93 -0.18315178533429588 1103 94-95 -0.4311530540377859 1103 96-97 -0.5266651338678869 1103 98-99 -0.825714397826232 1103 100-101 -0.9062574889589854 1104 1 0.31841924168344704 1104 2 0.21277291873693116 1104 3 0.30675168954998355 1104 4 0.2937423801063659 1104 5 0.2363005377399574 1104 6 0.2554725356970593 1104 7 0.33747447478156545 1104 8 0.3263421079676334 1104 9 0.41469728310358533 1104 10-11 0.368888956952226 1104 12-13 0.39490881969223324 1104 14-15 0.4141998507592817 1104 16-17 0.35199208897067535 1104 18-19 0.48561952655334295 1104 20-21 0.4416118633917918 1104 22-23 0.5636445891078026 1104 24-25 0.5602890355051358 1104 26-27 0.5946170354434059 1104 28-29 0.5989828736507974 1104 30-31 0.6271313255228961 1104 32-33 0.42387882394968557 1104 34-35 0.5371008884199782 1104 36-37 0.5889026207854329 1104 38-39 0.5234482898153843 1104 40-41 0.5832124234323786 1104 42-43 0.3653387021992458 1104 44-45 0.2964985961554518 1104 46-47 0.14283343794390646 1104 48-49 0.23211693992674043 1104 50-51 0.19663106058006719 1104 52-53 0.2989856191822682 1104 54-55 0.2256246477052244 1104 56-57 0.09878027721104843 1104 58-59 0.11775240883124383 1104 60-61 0.21153875567975433 1104 62-63 0.4586876879316115 1104 64-65 0.18934366442550754 1104 66-67 0.14202942158862086 1104 68-69 0.08378513731294746 1104 70-71 -0.10802822170763093 1104 72-73 -0.0530655697802942 1104 74-75 0.015173502980772469 1104 76-77 -0.23127777093733215 1104 78-79 -0.21272877781922261 1104 80-81 -0.2997311236419229 1104 82-83 -0.39618156752709055 1104 84-85 -0.21843389169999838 1104 86-87 -0.17665583493979042 1104 88-89 -0.3111016284427883 1104 90-91 -0.3451595517298536 1104 92-93 -0.49674475983753297 1104 94-95 -0.6545002287285584 1104 96-97 -0.812277699789778 1104 98-99 -0.7289271754746061 1104 100-101 -0.8629823430797376 1105 1 0.26929606782374194 1105 2 0.3016069769503815 1105 3 0.3817781755542242 1105 4 0.2384368152574723 1105 5 0.3367842558173493 1105 6 0.23916691508816257 1105 7 0.2885116109475092 1105 8 0.2964658951874384 1105 9 0.43381460216235723 1105 10-11 0.36854533589724525 1105 12-13 0.39519737773841257 1105 14-15 0.44845322423982736 1105 16-17 0.49546119792573506 1105 18-19 0.44019045328469275 1105 20-21 0.4350440313409365 1105 22-23 0.41899267096349746 1105 24-25 0.4399226922465189 1105 26-27 0.35199617562406615 1105 28-29 0.3280833253068707 1105 30-31 0.24043496665147757 1105 32-33 0.29939948303298536 1105 34-35 0.3303232593355574 1105 36-37 0.27675460584174516 1105 38-39 0.2989926469024837 1105 40-41 0.40890454454311964 1105 42-43 0.5121534116896029 1105 44-45 0.4285285392802436 1105 46-47 0.4769773824069361 1105 48-49 0.380137459610026 1105 50-51 0.42205065464720093 1105 52-53 0.41922719942092357 1105 54-55 0.43934230690004483 1105 56-57 0.43550381908681146 1105 58-59 0.4062010259830302 1105 60-61 0.45104820700127135 1105 62-63 0.46939639764500285 1105 64-65 0.2374882501286386 1105 66-67 0.051017935026784755 1105 68-69 0.11069073561784037 1105 70-71 -0.05748247062031098 1105 72-73 -0.057826777542086205 1105 74-75 -0.14859573422230454 1105 76-77 -0.31787445879280085 1105 78-79 -0.38421032125626553 1105 80-81 -0.30568001959374413 1105 82-83 -0.41173233701521283 1105 84-85 -0.2351946533817184 1105 86-87 -0.2418023443632329 1105 88-89 -0.1238030614750194 1105 90-91 -0.15910861496169915 1105 92-93 -0.5636316785394371 1105 94-95 -0.5787363166063315 1105 96-97 -0.8228616464832079 1105 98-99 -0.9610142726085407 1105 100-101 -1.2708849303441525 1106 1 0.17938781454622799 1106 2 0.1854658770894133 1106 3 0.16692981686224329 1106 4 0.25192821792975195 1106 5 0.25369298825154374 1106 6 0.28864985789570596 1106 7 0.32468636960178543 1106 8 0.10285340726454706 1106 9 0.362789049757545 1106 10-11 0.3655249795931965 1106 12-13 0.44293022039435215 1106 14-15 0.44691736996629317 1106 16-17 -0.09287290781864499 1106 18-19 0.3999073188144777 1106 20-21 0.5008381353572133 1106 22-23 0.45481847001617837 1106 24-25 0.5229087024423436 1106 26-27 0.38557484765309624 1106 28-29 0.30336306247764355 1106 30-31 0.2715745544196295 1106 32-33 0.1984401877860691 1106 34-35 0.1876999157645045 1106 36-37 0.2510367545060035 1106 38-39 0.1293719661857935 1106 40-41 0.07861673633147603 1106 42-43 0.0073275772892955615 1106 44-45 0.23737958439166817 1106 46-47 0.22888576136489291 1106 48-49 0.40708226625852006 1106 50-51 0.1217856456531976 1106 52-53 0.05647132189651671 1106 54-55 0.07377914069551395 1106 56-57 -0.14429069339090006 1106 58-59 -0.0067514236073336065 1106 60-61 0.21463524463470662 1106 62-63 0.12085635271424522 1106 64-65 0.01256866597380224 1106 66-67 -0.15885632561874274 1106 68-69 -0.134656731528505 1106 70-71 -0.43948149659688696 1106 72-73 -0.3753360268370969 1106 74-75 -0.2147199457739113 1106 76-77 -0.7263505245236708 1106 78-79 -0.5977994208344519 1106 80-81 -0.4241618194947705 1106 82-83 -0.48472685027842743 1106 84-85 -0.6099103013822216 1106 86-87 -0.5300680131795623 1106 88-89 -0.853034663036599 1106 90-91 -0.810589680417646 1106 92-93 -0.8419630939368616 1106 94-95 -1.3135608942022223 1106 96-97 -1.0635323283781553 1106 98-99 -1.0346099900474872 1106 100-101 -1.0588985509620272 1107 1 0.011914937548926474 1107 2 -0.0018410769954115835 1107 3 0.04636180973746917 1107 4 0.12049412638128132 1107 5 0.04048863388994306 1107 6 0.03259337062620915 1107 7 0.08244082593328272 1107 8 0.08533565176585256 1107 9 -0.06597286699398097 1107 10-11 0.08003604983763779 1107 12-13 0.021786179215141033 1107 14-15 -0.054273731703183614 1107 16-17 0.03372208659975229 1107 18-19 0.13033351134297533 1107 20-21 0.14960380681795726 1107 22-23 0.2971900515286947 1107 24-25 0.12173921879561078 1107 26-27 0.09566514235373091 1107 28-29 -0.04912164295477339 1107 30-31 -0.021255877883938012 1107 32-33 0.04065981918959949 1107 34-35 0.11937870571311038 1107 36-37 0.005152666380070059 1107 38-39 -0.05938521221813886 1107 40-41 -0.0037496737389304258 1107 42-43 -0.06524117050050648 1107 44-45 0.029109938279439973 1107 46-47 -0.10072916806365129 1107 48-49 -0.1789039697776218 1107 50-51 -0.31450771128256605 1107 52-53 -0.32630219701659513 1107 54-55 -0.3423677972620425 1107 56-57 -0.4958377308500843 1107 58-59 -0.4933292557380682 1107 60-61 -0.5779798332571566 1107 62-63 -0.4712025657569967 1107 64-65 -0.6527233232274661 1107 66-67 -0.7549323792174896 1107 68-69 -0.849859835421455 1107 70-71 -0.8222164774388752 1107 72-73 -0.7953116304029351 1107 74-75 -0.9318517883690127 1107 76-77 -1.0299796913837937 1107 78-79 -0.9033383326331048 1107 80-81 -1.01390570097956 1107 82-83 -1.0836422421454621 1107 84-85 -1.19629256197776 1107 86-87 -1.020428632262476 1107 88-89 -0.9242453108487254 1107 90-91 -1.0967513747861517 1107 92-93 -1.1987848922443831 1107 94-95 -1.2802172045269202 1107 96-97 -1.450228288872598 1107 98-99 -1.476175538626837 1107 100-101 -1.3790555954369808 1108 1 0.05097161846238052 1108 2 0.05267682508474536 1108 3 0.11360710042599464 1108 4 0.21795255497385568 1108 5 0.1857835518044766 1108 6 0.22967279862432122 1108 7 0.11692858771507986 1108 8 0.09297851713252925 1108 9 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-1.058431869122355 2316 68-69 -1.0872796691406101 2316 70-71 -1.0521538763942822 2316 72-73 -1.1174481325399093 2316 74-75 -1.1635507610611278 2316 76-77 -1.0411992085389201 2316 78-79 -0.8876997308021863 2316 80-81 -0.9090744052023325 2316 82-83 -1.1365615115400942 2316 84-85 -1.2437011092064694 2316 86-87 -1.1467961786879108 2316 88-89 -1.136527927280909 2316 90-91 -1.369939893069926 2316 92-93 -1.5359604903775335 2316 94-95 -1.5352236951062572 2316 96-97 -1.5010594558550352 2316 98-99 -1.7331120529654243 2316 100-101 -1.1465283366984238 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8708.0 3 2679.0 4 2588.0 5 2885.0 6 3335.0 7 4290.0 8 5524.0 9 6832.0 10 8666.0 11 10596.0 12 13449.0 13 16604.0 14 19200.0 15 23084.0 16 26941.0 17 32805.0 18 32731.0 19 29212.0 20 29406.0 21 31223.0 22 34362.0 23 37845.0 24 42732.0 25 47377.0 26 53255.0 27 60110.0 28 68792.0 29 78832.0 30 91808.0 31 108107.0 32 129552.0 33 159850.0 34 200675.0 35 255183.0 36 305950.0 37 307273.0 38 144130.0 39 7887.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.45403648665866 18.537559398053297 14.99835924335924 23.010044871928805 2 15.369952959022 19.551455085955283 40.84121203885577 24.237379916166944 3 19.32232057142787 23.69501557594106 29.672997427296227 27.309666425334843 4 12.744327377487616 16.674866041602577 37.04338322731493 33.53742335359487 5 13.833588837605856 37.03396430591649 34.2644756150548 14.867971241422845 6 32.29868684529287 36.04340153594215 17.23514015933299 14.422771459431985 7 28.547168682450348 32.737736667101046 20.24905726563185 18.46603738481675 8 26.278862214653 34.967535125929324 19.373874168931977 19.379728490485704 9 25.999542055335933 17.056554527569457 20.247216388684425 36.69668702841018 10-11 24.968349564142322 26.5739168466317 26.987036169849866 21.470697419376105 12-13 26.087718094200103 23.795640625624205 27.483316107395613 22.633325172780083 14-15 22.711311891673542 25.318609637849367 25.949171582167036 26.02090688831005 16-17 22.055223884877115 28.447006954910858 26.876140153518485 22.621629006693546 18-19 22.026266416510317 26.692467453730632 29.46699083215219 21.814275297606855 20-21 22.5200784642891 25.882134381053213 29.949852741201187 21.6479344134565 22-23 22.22031385687082 25.83419534287347 29.809331756267493 22.136159043988208 24-25 22.107081103630083 25.991617921535624 29.852662780722245 22.048638194112048 26-27 21.684217901070937 26.30679636948998 29.67322869648335 22.335757032955733 28-29 21.853686950238522 26.804643126614415 29.44935579034984 21.89231413279722 30-31 21.902480063405626 26.285367505106866 29.438040797205794 22.374111634281707 32-33 21.607439165805463 26.305620376275574 29.431986168302853 22.65495428961611 34-35 22.422434411518662 26.41480345874286 29.19083073624608 21.971931393492397 36-37 22.49161399288584 25.95746858766026 29.256063669166004 22.294853750287892 38-39 21.946197059154585 26.415361159147423 29.123353832615344 22.51508794908265 40-41 22.445757132436675 26.24214386118017 29.199232581005113 22.11286642537804 42-43 22.148390025571786 26.21955138241953 28.995053884131615 22.637004707877068 44-45 22.20046545253933 26.479207392399655 29.095244653158463 22.225082501902552 46-47 22.593595774312085 26.792494533190215 28.31280486993751 22.301104822560198 48-49 22.358896324451177 26.844537631125625 28.249486465989705 22.547079578433497 50-51 22.488248911257816 27.083877815282936 28.207238306369664 22.220634967089584 52-53 22.82071013096414 26.757102424726153 28.1349583971097 22.287229047200004 54-55 22.353300161819085 26.987522923688395 28.27753231930526 22.381644595187257 56-57 22.402479757343876 27.13936870533956 28.022260527971437 22.435891009345124 58-59 22.406466535388756 26.77211876571477 28.058934258582006 22.762480440314462 60-61 22.108914458788608 26.931996039355173 28.385153270365983 22.573936231490237 62-63 22.215741411561492 26.861131917924286 28.53107309945111 22.392053571063112 64-65 21.94997227275425 27.06848322757418 28.28044011631119 22.70110438336038 66-67 21.916848716213064 27.060222453768688 28.1768498457524 22.846078984265844 68-69 22.47976793662633 26.733587660948732 28.29674457104423 22.489899831380708 70-71 22.389784891772578 26.826835922494812 27.913765972874128 22.869613212858482 72-73 22.278565153068335 26.986069552571685 27.87472932233425 22.86063597202573 74-75 22.28422208711763 27.350432772380984 27.71650436721837 22.648840773283016 76-77 22.46885599605062 26.975634935459475 27.80356389167527 22.751945176814633 78-79 22.57798149746244 27.164415943329484 27.67718640964924 22.58041614955884 80-81 22.73626026480202 27.207454165418604 27.38455627083855 22.671729298940825 82-83 22.698665029921745 27.245931154416652 27.478451230116107 22.576952585545495 84-85 22.67568180218694 27.41584982430183 27.365542209296045 22.542926164215185 86-87 22.89930339594477 27.32282507643658 27.13173362096621 22.646137906652438 88-89 22.78348774639484 27.409964375663286 26.93246931609665 22.87407856184522 90-91 22.671551763680565 27.48790602659118 26.963970361778948 22.876571847949307 92-93 22.864072144603647 27.050561981765686 27.1856529650004 22.899712908630264 94-95 22.746282894097153 26.87429442283148 27.23114887153711 23.148273811534263 96-97 23.068855368888077 26.526074520619275 27.227650220833826 23.17741988965882 98-99 22.750571463246764 26.84965497311682 26.80514131704345 23.594632246592965 100-101 23.728697611305975 25.59317954888758 27.05500908881436 23.62311375099209 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 888.0 1 604.0 2 429.5 3 566.0 4 503.0 5 497.0 6 901.5 7 1962.5 8 3277.0 9 3771.5 10 3064.5 11 2287.0 12 2241.0 13 2422.5 14 2641.0 15 2818.5 16 3026.0 17 3330.0 18 3610.0 19 4003.5 20 4545.0 21 5429.0 22 6701.5 23 8276.5 24 10270.0 25 12808.0 26 16103.5 27 19812.0 28 23331.5 29 26635.5 30 30174.0 31 35360.5 32 41905.5 33 44874.0 34 45688.5 35 48739.5 36 52918.5 37 57069.0 38 60747.0 39 63999.0 40 67697.5 41 72286.0 42 74949.5 43 76690.5 44 80150.0 45 84848.0 46 87402.5 47 88553.5 48 89083.0 49 87631.5 50 86073.0 51 84585.5 52 83336.0 53 83243.0 54 83670.0 55 80191.5 56 74228.5 57 68257.0 58 63418.0 59 56926.0 60 46695.0 61 38607.5 62 32286.0 63 24685.5 64 17892.0 65 13433.0 66 10001.0 67 7558.0 68 5880.0 69 4444.5 70 3379.5 71 2469.5 72 1746.5 73 1302.5 74 972.0 75 690.5 76 479.5 77 326.5 78 212.0 79 127.0 80 74.0 81 52.5 82 41.0 83 27.5 84 22.5 85 17.0 86 14.0 87 8.5 88 8.0 89 6.0 90 1.0 91 2.5 92 3.5 93 1.5 94 0.0 95 0.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14444801712267405 2 0.05273109432770514 3 0.15770238063095682 4 0.13233909243609474 5 0.19873363556554813 6 0.20180177526653953 7 0.17328853031199298 8 0.07518987693896202 9 0.12824823950143957 10-11 0.10507355762661803 12-13 0.15854100548256111 14-15 0.14563436447372405 16-17 0.14191168830318784 18-19 0.13655267095878956 20-21 0.13775947257451285 22-23 0.20153586982578695 24-25 0.2186765436219921 26-27 0.15213882063982576 28-29 0.13058002567419302 30-31 0.09914182087136804 32-33 0.12800278832536027 34-35 0.0948668795546534 36-37 0.0885874202999577 38-39 0.05330381373855686 40-41 0.06070825755028272 42-43 0.06344912901650168 44-45 0.042933501549206006 46-47 0.05422425564885427 48-49 0.04561301022140514 50-51 0.05753784652592496 52-53 0.029351869806150843 54-55 0.05389698741408186 56-57 0.028247339513793945 58-59 0.053794716090715476 60-61 0.038310837733045666 62-63 0.055962868146082714 64-65 0.0422585108149879 66-67 0.041072163463937904 68-69 0.06942177430109824 70-71 0.0536924447673491 72-73 0.053365176532576684 74-75 0.045142562133919796 76-77 0.022438328346583606 78-79 0.024422392019891367 80-81 0.028226885249120672 82-83 0.024667843195970672 84-85 0.02102698408412757 86-87 0.02544510525355515 88-89 0.06649681445281978 90-91 0.11315299217256199 92-93 0.08386248516043097 94-95 0.07713303208292323 96-97 0.06917632312501892 98-99 0.05757875505527151 100-101 0.13460951581482836 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2444478.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.2282889945542 #Duplication Level Percentage of deduplicated Percentage of total 1 77.47625823748963 44.33833696631767 2 13.006264905518947 14.886525735055328 3 4.132811825782102 7.095412485779079 4 1.8414004362606304 4.215207852840861 5 1.0009273372565364 2.864067945953334 6 0.5935133900308438 2.0379453484093615 7 0.37662189410125846 1.5087398618112244 8 0.2688973914458684 1.2310830102036736 9 0.19294976148378104 0.9937966240461713 >10 1.0073240738818412 10.35267028078375 >50 0.06534556639231766 2.5691572217979393 >100 0.03188976881448993 3.401865303678389 >500 0.003219673078787663 1.24671962255567 >1k 0.0023610935911082633 2.2373638207969826 >5k 1.4309658127928867E-4 0.5827824615022045 >10k+ 7.154829063964434E-5 0.43832545846842536 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10705 0.43792580665483594 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8758 0.3582769000170998 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5475 0.22397419817237052 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2988 0.12223468568749647 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2773 0.11343935187798784 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2768 0.1132348092312551 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2723 0.11139392541066027 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2514 0.10284404277723096 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.0908529346551695E-5 2 0.0 0.0 0.0 0.0 4.0908529346551695E-5 3 0.0 0.0 0.0 0.0 4.0908529346551695E-5 4 0.0 0.0 0.0 0.0 4.0908529346551695E-5 5 0.0 0.0 0.0 4.0908529346551695E-5 4.0908529346551695E-5 6 0.0 0.0 0.0 4.0908529346551695E-5 8.181705869310339E-5 7 0.0 0.0 0.0 4.0908529346551695E-5 8.181705869310339E-5 8 0.0 0.0 0.0 4.0908529346551695E-5 8.181705869310339E-5 9 0.0 0.0 0.0 4.0908529346551695E-5 8.181705869310339E-5 10-11 0.0 0.0 0.0 1.4317985271293092E-4 8.181705869310339E-5 12-13 0.0 0.0 0.0 1.6363411738620678E-4 2.454511760793102E-4 14-15 0.0 0.0 0.0 1.6363411738620678E-4 3.2726823477241356E-4 16-17 0.0 0.0 0.0 1.8408838205948263E-4 4.09085293465517E-4 18-19 0.0 0.0 0.0 2.045426467327585E-4 4.09085293465517E-4 20-21 0.0 0.0 0.0 3.2726823477241356E-4 5.318108815051721E-4 22-23 4.0908529346551695E-5 0.0 0.0 3.886310287922411E-4 5.727194108517238E-4 24-25 4.0908529346551695E-5 0.0 0.0 6.954449988913788E-4 5.727194108517238E-4 26-27 4.0908529346551695E-5 4.0908529346551695E-5 0.0 0.0012477101450698268 5.727194108517238E-4 28-29 6.136279401982754E-5 4.0908529346551695E-5 0.0 0.003231773818377584 5.727194108517238E-4 30-31 8.181705869310339E-5 4.0908529346551695E-5 0.0 0.010083952483924994 5.727194108517238E-4 32-33 8.181705869310339E-5 4.0908529346551695E-5 0.0 0.02403376099109912 6.136279401982754E-4 34-35 8.181705869310339E-5 4.0908529346551695E-5 0.0 0.04551073889803876 6.136279401982754E-4 36-37 8.181705869310339E-5 4.0908529346551695E-5 0.0 0.08036480590130081 6.136279401982754E-4 38-39 1.0227132336637924E-4 4.0908529346551695E-5 0.0 0.13004821479268786 6.74990734218103E-4 40-41 1.227255880396551E-4 4.0908529346551695E-5 0.0 0.1838633851480766 6.954449988913788E-4 42-43 1.4317985271293092E-4 4.0908529346551695E-5 0.0 0.23767855550346534 6.954449988913788E-4 44-45 1.6363411738620678E-4 4.0908529346551695E-5 0.0 0.3053208087779886 7.568077929112064E-4 46-47 1.6363411738620678E-4 4.0908529346551695E-5 0.0 0.38411063629944714 8.590791162775857E-4 48-49 1.6363411738620678E-4 4.0908529346551695E-5 0.0 0.46787085013651175 9.613504396439649E-4 50-51 1.6363411738620678E-4 4.0908529346551695E-5 0.0 0.5564173619071229 0.0010227132336637925 52-53 1.8408838205948263E-4 4.0908529346551695E-5 0.0 0.6424684533875944 0.0010636217630103442 54-55 2.045426467327585E-4 4.0908529346551695E-5 0.0 0.7346353700053754 0.0010636217630103442 56-57 2.045426467327585E-4 4.0908529346551695E-5 0.0 0.8441475030660943 0.0010636217630103442 58-59 2.045426467327585E-4 4.0908529346551695E-5 0.0 0.9600004581755287 0.0010636217630103442 60-61 2.045426467327585E-4 4.0908529346551695E-5 0.0 1.0828692260678967 0.0010636217630103442 62-63 2.045426467327585E-4 4.0908529346551695E-5 0.0 1.202874396906006 0.0011454388217034476 64-65 2.045426467327585E-4 4.0908529346551695E-5 0.0 1.3260090702391267 0.001206801615723275 66-67 2.045426467327585E-4 4.0908529346551695E-5 0.0 1.4656912436929275 0.0012272558803965508 68-69 2.045426467327585E-4 4.0908529346551695E-5 0.0 1.6211641094744973 0.0012477101450698268 70-71 2.045426467327585E-4 4.0908529346551695E-5 0.0 1.7841845989205058 0.0012681644097431025 72-73 2.045426467327585E-4 4.0908529346551695E-5 0.0 1.9491277892458023 0.001349981468436206 74-75 2.045426467327585E-4 4.0908529346551695E-5 0.0 2.1144187020705445 0.001472707056475861 76-77 2.045426467327585E-4 4.0908529346551695E-5 0.0 2.293516243549748 0.0015545241151689645 78-79 2.045426467327585E-4 4.0908529346551695E-5 0.0 2.5000224996911404 0.0015954326445155162 80-81 2.045426467327585E-4 4.0908529346551695E-5 0.0 2.715753629200181 0.001636341173862068 82-83 2.045426467327585E-4 4.0908529346551695E-5 0.0 2.9334074595885093 0.0018204295559215507 84-85 2.045426467327585E-4 4.0908529346551695E-5 0.0 3.1522885458572345 0.0018408838205948264 86-87 2.045426467327585E-4 4.0908529346551695E-5 0.0 3.3827467459310334 0.0019227008792879296 88-89 2.045426467327585E-4 4.0908529346551695E-5 0.0 3.625968407160956 0.002004517937981033 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5170 0.0 56.46151 1 GTATCAA 9075 0.0 41.92593 1 ATCAACG 11735 0.0 31.940691 3 TCAACGC 11855 0.0 31.697624 4 TATCAAC 11980 0.0 31.286837 2 CAACGCA 11965 0.0 31.247196 5 AACGCAG 12425 0.0 30.124313 6 TGGTATC 2170 0.0 28.933865 2 GTGGTAT 2275 0.0 28.048265 1 ACGCAGA 13955 0.0 26.645672 7 CGCAGAG 13995 0.0 26.395302 8 TAAGGTG 1640 0.0 26.103567 5 GTAAGGT 1510 0.0 25.515802 4 GCAGAGT 15120 0.0 23.960209 9 AGGTAAG 1600 0.0 23.188192 2 GAGTACT 10300 0.0 22.72972 12-13 GTACATG 7620 0.0 22.3723 1 GGTAAGG 1730 0.0 21.996067 3 CAGAGTA 14525 0.0 21.716505 10-11 AAGGTAA 1630 0.0 21.618557 1 >>END_MODULE