FastQCFastQC Report
Thu 26 May 2016
SRR938000_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938000_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2444478
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103310.4226260166792256No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88580.36236775295175494No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57860.23669675079914812No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC29120.11912563745715854No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA28700.11740747922460337No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA28400.11618022334420681No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA27340.11184391923347234No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA26560.1086530539444413No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50350.054.6293221
GTATCAA86100.039.781291
ATCAACG111550.030.6983283
TCAACGC112650.030.3539184
TATCAAC112450.030.3308832
CAACGCA114050.029.9813165
GTGGTAT23850.029.877871
AACGCAG118550.028.8032056
TGGTATC23600.028.582252
ACGCAGA133250.025.4458987
CGCAGAG133950.025.2065668
TAAGGTG15900.023.2975865
GCAGAGT148000.022.4286069
GAGTACT100800.021.71925712-13
GTACATG78600.021.2748661
GTACTTT102650.021.21216214-15
TACATGG76600.020.7127512
GTAAGGT16300.020.6863654
CAGAGTA144300.020.40468810-11
AGAGTAC134000.020.32512310-11
Produced by FastQC (version 0.11.5)