##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938000_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2444478 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.52895546615678 34.0 31.0 34.0 31.0 34.0 2 32.74201117784656 34.0 31.0 34.0 31.0 34.0 3 32.79769668616367 34.0 31.0 34.0 31.0 34.0 4 36.24785700669018 37.0 35.0 37.0 35.0 37.0 5 36.22392797153421 37.0 37.0 37.0 35.0 37.0 6 36.256383162376586 37.0 37.0 37.0 35.0 37.0 7 36.21631775781987 37.0 37.0 37.0 35.0 37.0 8 36.203673340484144 37.0 37.0 37.0 35.0 37.0 9 37.898393031150206 39.0 38.0 39.0 35.0 39.0 10-11 37.873488941197266 39.0 38.0 39.0 35.0 39.0 12-13 37.79285107086257 39.0 38.0 39.0 35.0 39.0 14-15 39.07501315209218 40.0 38.5 41.0 36.0 41.0 16-17 38.95566210863833 40.0 38.0 41.0 36.0 41.0 18-19 39.21173395710659 40.5 39.0 41.0 36.0 41.0 20-21 39.19008045889552 40.0 39.0 41.0 36.0 41.0 22-23 39.09877773496018 40.0 39.0 41.0 35.5 41.0 24-25 38.99584144344928 40.0 38.5 41.0 35.0 41.0 26-27 38.819444683077535 40.0 38.0 41.0 35.0 41.0 28-29 38.70033131817918 40.0 38.0 41.0 34.5 41.0 30-31 38.450484520621586 40.0 38.0 41.0 34.0 41.0 32-33 38.2261133051719 40.0 38.0 41.0 34.0 41.0 34-35 38.065835937161225 40.0 38.0 41.0 34.0 41.0 36-37 38.053144679559395 40.0 38.0 41.0 33.5 41.0 38-39 37.896202379403704 40.0 37.5 41.0 33.0 41.0 40-41 37.71307637049709 40.0 37.0 41.0 33.0 41.0 42-43 37.476036397136724 40.0 37.0 41.0 32.5 41.0 44-45 37.19371129541767 40.0 37.0 41.0 31.5 41.0 46-47 36.79159661081016 40.0 36.0 41.0 31.0 41.0 48-49 36.38020325811891 39.0 35.5 41.0 30.0 41.0 50-51 35.98201558778602 39.0 35.0 40.5 29.0 41.0 52-53 35.448680863562686 38.5 35.0 40.0 27.5 41.0 54-55 35.12251777271057 38.0 34.5 40.0 27.0 41.0 56-57 34.75375560753666 38.0 34.0 40.0 26.0 41.0 58-59 34.29886217016475 37.5 33.5 40.0 25.5 41.0 60-61 33.798889987964714 37.0 33.0 40.0 23.5 41.0 62-63 33.321047683799975 36.5 32.5 39.0 22.5 41.0 64-65 32.81792145398731 36.0 32.0 39.0 21.0 40.0 66-67 32.24808323085747 35.0 31.0 38.5 18.5 40.0 68-69 31.642960582995634 35.0 31.0 38.0 16.0 40.0 70-71 31.00137534475663 35.0 30.0 37.5 10.0 39.5 72-73 30.395009486687954 34.0 29.0 37.0 7.5 39.0 74-75 29.773042138239738 34.0 29.0 36.0 6.0 39.0 76-77 28.42015595967728 32.5 27.0 35.0 2.0 37.0 78-79 29.089115549413822 34.0 29.0 35.0 2.0 37.0 80-81 28.746562660821656 34.0 28.5 35.0 2.0 37.0 82-83 28.15721638730232 33.5 27.0 35.0 2.0 36.5 84-85 27.532202785216313 33.0 26.0 35.0 2.0 36.0 86-87 26.866733715746264 32.5 25.5 35.0 2.0 35.5 88-89 26.087786226752705 32.0 23.5 34.5 2.0 35.0 90-91 25.49497540988301 32.0 21.5 34.0 2.0 35.0 92-93 24.65009257600191 31.0 18.5 34.0 2.0 35.0 94-95 23.781163708570908 31.0 6.0 34.0 2.0 35.0 96-97 22.685674405742247 30.5 2.0 34.0 2.0 35.0 98-99 21.175080732982664 29.5 2.0 34.0 2.0 35.0 100-101 16.82199655713817 21.5 2.0 30.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.0983875232400564 1101 2 0.11599465502583683 1101 3 0.17140465659443294 1101 4 0.11065427130777294 1101 5 -0.013585772918467853 1101 6 0.07306142731326304 1101 7 0.03112118739416303 1101 8 0.06222229971520221 1101 9 -0.005173092219074249 1101 10-11 0.15031804650213587 1101 12-13 0.1798121660480021 1101 14-15 0.032666768929423995 1101 16-17 0.18843275415495242 1101 18-19 0.19034764997443432 1101 20-21 0.21983309932891615 1101 22-23 0.2166191323493436 1101 24-25 0.21496445117175256 1101 26-27 0.24754544472907725 1101 28-29 0.26549846033817914 1101 30-31 0.19413294764911626 1101 32-33 0.23821025507144356 1101 34-35 0.247094304841049 1101 36-37 0.36214165211480775 1101 38-39 0.30354814188866186 1101 40-41 0.22254449514376518 1101 42-43 0.31550825206832656 1101 44-45 0.2992865199879162 1101 46-47 0.38917711836322866 1101 48-49 0.40249677572256815 1101 50-51 0.2609395892604667 1101 52-53 0.08319336082642792 1101 54-55 0.04262492213451452 1101 56-57 0.21230794230215366 1101 58-59 0.1923548949863516 1101 60-61 0.12517982318572507 1101 62-63 0.1741815124243118 1101 64-65 0.17700041571941938 1101 66-67 0.09372974739193296 1101 68-69 -0.04990039890516229 1101 70-71 -0.09428497311845518 1101 72-73 -0.13136808902033792 1101 74-75 -0.3680423413170608 1101 76-77 -0.3609251553572079 1101 78-79 -0.39354931103157753 1101 80-81 -0.24280812558116338 1101 82-83 -0.2388965568147512 1101 84-85 -0.3818022847568656 1101 86-87 -0.6382206943070052 1101 88-89 -0.5732096426106388 1101 90-91 -0.6413091347982771 1101 92-93 -0.6942284197141007 1101 94-95 -0.7054276069345065 1101 96-97 -0.8239652053597126 1101 98-99 -0.8482231266753182 1101 100-101 -1.4474965470627623 1102 1 0.1780343799749886 1102 2 0.17300583901132427 1102 3 0.1750874151125572 1102 4 0.11545059486999065 1102 5 0.10901234722014408 1102 6 0.06262663500522336 1102 7 0.06692258241788807 1102 8 0.14350697081381725 1102 9 0.18781572379543832 1102 10-11 0.201608963975616 1102 12-13 0.19021163659336793 1102 14-15 0.23883339097913137 1102 16-17 0.24755952582020768 1102 18-19 0.28599340615760127 1102 20-21 0.10264199743864566 1102 22-23 0.2542507523912789 1102 24-25 0.23090479085556836 1102 26-27 0.1909097868519538 1102 28-29 0.05212062158388875 1102 30-31 0.07466173302665169 1102 32-33 -0.005561960458244641 1102 34-35 0.03913092049565847 1102 36-37 0.11644347099967689 1102 38-39 0.056352503940452436 1102 40-41 0.10748106469416996 1102 42-43 -0.006840942595481181 1102 44-45 -0.07000064966064201 1102 46-47 -0.10059165422411098 1102 48-49 -0.04616768984056563 1102 50-51 0.06478018028651178 1102 52-53 -4.337008490864491E-4 1102 54-55 0.18542987907914465 1102 56-57 0.16754969188200874 1102 58-59 0.24346362652607922 1102 60-61 0.08811892846687641 1102 62-63 0.11891968521094043 1102 64-65 0.09671962629661124 1102 66-67 0.0605821321686264 1102 68-69 0.19304919136239107 1102 70-71 -0.02036396001593843 1102 72-73 -0.03127364978120539 1102 74-75 0.1720199737084478 1102 76-77 0.06359502638255776 1102 78-79 0.02748609996501372 1102 80-81 0.006175908982108069 1102 82-83 -0.18362171632039548 1102 84-85 -0.28616582377440025 1102 86-87 -0.30140723783085477 1102 88-89 -0.3596217323204769 1102 90-91 -0.574838225058393 1102 92-93 -0.6372572050916325 1102 94-95 -0.6488096099387128 1102 96-97 -0.7522713223453898 1102 98-99 -0.428597797625212 1102 100-101 -0.6030033957666383 1103 1 0.1885538100227251 1103 2 0.18283926202906287 1103 3 0.1833057321296181 1103 4 0.11139906252539333 1103 5 0.03728488396714624 1103 6 0.08542496152568901 1103 7 0.05577418147510116 1103 8 0.0796044719230693 1103 9 0.15196044925102115 1103 10-11 0.1257383172094535 1103 12-13 0.1256994410876615 1103 14-15 0.22804746450776037 1103 16-17 0.25659916318052467 1103 18-19 0.24373592380339915 1103 20-21 0.2030041572279373 1103 22-23 0.24735705043930523 1103 24-25 0.3139763479971265 1103 26-27 0.40504035161196583 1103 28-29 0.3595640732062222 1103 30-31 0.32673037463833055 1103 32-33 0.39078174794472176 1103 34-35 0.348439847083462 1103 36-37 0.34292806258948616 1103 38-39 0.30947277387477357 1103 40-41 0.2762183397357916 1103 42-43 0.23689786652148825 1103 44-45 0.2478550434109934 1103 46-47 0.33566063115481626 1103 48-49 0.43088647639341104 1103 50-51 0.19521965747362913 1103 52-53 0.09990977468910245 1103 54-55 0.08545163148095725 1103 56-57 0.2719840350302789 1103 58-59 0.4988974000997075 1103 60-61 0.23687644543611697 1103 62-63 0.13305284609846524 1103 64-65 0.08387115517970756 1103 66-67 0.08588788518721202 1103 68-69 0.11121433496758115 1103 70-71 -0.09047631726826211 1103 72-73 -0.13407321929605942 1103 74-75 -0.17772167226786806 1103 76-77 -0.0997578408436155 1103 78-79 -0.21132065688954782 1103 80-81 -0.1379589275877322 1103 82-83 -0.2631540829502441 1103 84-85 -0.2647391179612768 1103 86-87 -0.2754813643519505 1103 88-89 -0.6123321287884842 1103 90-91 -0.7854339261858989 1103 92-93 -0.6693290171257082 1103 94-95 -0.8679206093376699 1103 96-97 -0.9724610914001808 1103 98-99 -1.0846299785926092 1103 100-101 -1.7115714819643664 1104 1 0.15329679676965924 1104 2 0.17082512801724903 1104 3 0.1346459697171909 1104 4 0.12610270517647848 1104 5 0.08385873738911442 1104 6 0.06972651796916551 1104 7 0.0631189104114469 1104 8 0.10854701909119058 1104 9 0.15456559578724693 1104 10-11 0.1346446782613384 1104 12-13 0.21449204768253338 1104 14-15 0.21284772789669404 1104 16-17 0.1288730727443479 1104 18-19 0.19522416835495449 1104 20-21 0.2686709090032693 1104 22-23 0.21935480476491165 1104 24-25 0.23605712060467 1104 26-27 0.25491388311412067 1104 28-29 0.13014618664862354 1104 30-31 0.09805980193163322 1104 32-33 0.16704337199733033 1104 34-35 0.20457465175159228 1104 36-37 0.24180235111087 1104 38-39 0.22858295526076233 1104 40-41 0.2717910091020457 1104 42-43 0.3660834959971595 1104 44-45 0.3812899472232658 1104 46-47 0.45793323422589793 1104 48-49 0.36579924747199044 1104 50-51 0.3437466055663023 1104 52-53 0.32852678119319734 1104 54-55 0.3540555282635509 1104 56-57 0.46480985938988084 1104 58-59 0.5320466672692632 1104 60-61 0.3163461064348425 1104 62-63 0.4155260965926999 1104 64-65 0.46768736823209167 1104 66-67 0.42913600533078977 1104 68-69 0.36322465402057347 1104 70-71 0.2535850012890144 1104 72-73 -0.05759990609017862 1104 74-75 -0.07829776091228169 1104 76-77 0.02353531970418743 1104 78-79 -0.06544327608648359 1104 80-81 -0.00322126386552668 1104 82-83 -0.20171887453852122 1104 84-85 -0.12094839271595603 1104 86-87 -0.29192320228115065 1104 88-89 -0.30569828121960896 1104 90-91 -0.4367082418823287 1104 92-93 -0.5569846390759814 1104 94-95 -0.447761314276363 1104 96-97 -0.607011740017839 1104 98-99 -0.5364149836393999 1104 100-101 -1.0803230870840252 1105 1 0.17295916991564297 1105 2 0.20676663576788457 1105 3 0.15101432033613094 1105 4 0.1007346891176013 1105 5 0.18253164917678077 1105 6 0.14290455967765325 1105 7 0.12488427229390453 1105 8 0.10132470393562443 1105 9 0.15004764106437563 1105 10-11 0.12790677818327367 1105 12-13 0.160984743121368 1105 14-15 0.20137231806062772 1105 16-17 0.17519848815641836 1105 18-19 0.19626060752077734 1105 20-21 0.15366658760257934 1105 22-23 0.1933776106085645 1105 24-25 0.2061390878177889 1105 26-27 0.17831357085806587 1105 28-29 0.14774683346472273 1105 30-31 0.25136497646045797 1105 32-33 0.24020747370358464 1105 34-35 0.27472422797553264 1105 36-37 0.26418790369255163 1105 38-39 0.32940541539235824 1105 40-41 0.35179477290264316 1105 42-43 0.295647452727394 1105 44-45 0.1864289290455048 1105 46-47 0.18242512114459686 1105 48-49 0.2630154290265807 1105 50-51 0.3134647387212013 1105 52-53 0.2679567745019895 1105 54-55 0.2061346795962109 1105 56-57 0.3478107236700154 1105 58-59 0.30057920323447007 1105 60-61 0.3638306330323715 1105 62-63 0.24388075812944265 1105 64-65 0.22492780539056412 1105 66-67 0.1485140024312699 1105 68-69 0.18729973487258533 1105 70-71 0.05258759412942382 1105 72-73 0.16024538776506603 1105 74-75 0.09345630956836715 1105 76-77 0.04748215253567878 1105 78-79 0.1320266135086996 1105 80-81 0.031963998764119594 1105 82-83 -0.09194623597489482 1105 84-85 -0.09175663404527512 1105 86-87 -0.2905884470675879 1105 88-89 -0.24561826560680444 1105 90-91 -0.20150694825012394 1105 92-93 -0.6161007035345527 1105 94-95 -0.6564187526615513 1105 96-97 -0.7581889732061953 1105 98-99 -0.9591643702793071 1105 100-101 -1.638045848319667 1106 1 0.14887172774827917 1106 2 0.11659995208199092 1106 3 0.09349961179692912 1106 4 0.11655746398465539 1106 5 0.11357786030421124 1106 6 0.1313802522947114 1106 7 0.18298712602543787 1106 8 0.19536666549311832 1106 9 0.23561010031642127 1106 10-11 0.183920096382586 1106 12-13 0.1556500993076 1106 14-15 0.1993414988598019 1106 16-17 0.3135906229172747 1106 18-19 0.2651039350675788 1106 20-21 0.3018813362024204 1106 22-23 0.3458432055876344 1106 24-25 0.2934361335419027 1106 26-27 0.22128449940088046 1106 28-29 0.17231971633840004 1106 30-31 0.32584094793100604 1106 32-33 0.2295030348274807 1106 34-35 0.31252802116836165 1106 36-37 0.34456241177144875 1106 38-39 0.2763254590663067 1106 40-41 0.24610203801768193 1106 42-43 0.2532227431670364 1106 44-45 0.11391308152730062 1106 46-47 0.07532142692510746 1106 48-49 0.07622807640653662 1106 50-51 0.061061885775174574 1106 52-53 0.0864087252980994 1106 54-55 -0.2699011039820647 1106 56-57 -0.23801694095172365 1106 58-59 0.0034026929090344993 1106 60-61 -0.17331932729974397 1106 62-63 -0.08077744998165315 1106 64-65 -0.10258894545581398 1106 66-67 -0.1540565553542379 1106 68-69 -0.25377096430342405 1106 70-71 -0.7890113556533827 1106 72-73 -0.6458887014837593 1106 74-75 -0.5243909022895785 1106 76-77 -0.37694177937694207 1106 78-79 -0.34522738666411 1106 80-81 -0.43171415718960304 1106 82-83 -0.4798828773221899 1106 84-85 -0.43969861681134503 1106 86-87 -0.38902077993223827 1106 88-89 -0.6231082111714628 1106 90-91 -0.7512890694217766 1106 92-93 -0.9216612775799682 1106 94-95 -1.3484403029298022 1106 96-97 -1.5496153087572608 1106 98-99 -1.2904809907322239 1106 100-101 -1.784982682115137 1107 1 0.02284709678242791 1107 2 0.009473657133987956 1107 3 0.0038668484171964224 1107 4 -0.01360156795368539 1107 5 -0.03493355164446399 1107 6 -0.028957392592381836 1107 7 -0.04045844546387656 1107 8 -0.01333772676425582 1107 9 -0.04142412717968824 1107 10-11 0.0504855119138341 1107 12-13 0.0063125962773114 1107 14-15 0.024055119700598482 1107 16-17 -0.14568078365075365 1107 18-19 -0.002127124709190298 1107 20-21 0.0028957316401019284 1107 22-23 -0.028639030839030966 1107 24-25 -0.044007988548912635 1107 26-27 0.016228194361403325 1107 28-29 -0.016885145991672346 1107 30-31 0.030483978036393466 1107 32-33 0.0054707670840414835 1107 34-35 0.08851291204230449 1107 36-37 -0.024771872412323148 1107 38-39 8.217435374646698E-4 1107 40-41 -0.04467743484390496 1107 42-43 0.07988932664041215 1107 44-45 -0.0039545996571490605 1107 46-47 -0.13144651901588844 1107 48-49 -0.21594739220264358 1107 50-51 -0.2708817524793332 1107 52-53 -0.13671129124757897 1107 54-55 -0.07707091024751378 1107 56-57 -0.20202265779929718 1107 58-59 -0.15521909024017333 1107 60-61 -0.2660508268693107 1107 62-63 -0.19806669307008207 1107 64-65 -0.4738420585520231 1107 66-67 -0.5804514087233024 1107 68-69 -0.49352437937294624 1107 70-71 -0.5709263380992908 1107 72-73 -0.7171281610919102 1107 74-75 -0.6926383027590468 1107 76-77 -0.7400547342798731 1107 78-79 -0.7692615996625598 1107 80-81 -0.5660273179225364 1107 82-83 -0.5140233874965183 1107 84-85 -0.7760830337037206 1107 86-87 -0.8894302010592128 1107 88-89 -1.139462483951636 1107 90-91 -1.3109365525095278 1107 92-93 -1.340316034636885 1107 94-95 -1.5231423715249974 1107 96-97 -1.5958176846476988 1107 98-99 -1.6144815655000286 1107 100-101 -1.9098162114115045 1108 1 0.016592882074945692 1108 2 0.042559404549948 1108 3 0.06385124078267523 1108 4 0.06490169102848853 1108 5 0.03928891559536396 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-0.13155754439915057 2316 56-57 -0.058090552987458466 2316 58-59 -0.06844299319669744 2316 60-61 0.17924772283361534 2316 62-63 0.24455136599249272 2316 64-65 0.3375398756067227 2316 66-67 0.32949615824151834 2316 68-69 0.47587763999372257 2316 70-71 0.6045297818561046 2316 72-73 0.9086093076838253 2316 74-75 1.1595313131952345 2316 76-77 1.0406371648670287 2316 78-79 0.9190939281757551 2316 80-81 0.8478025306080177 2316 82-83 1.2329645430763136 2316 84-85 1.3839099842631555 2316 86-87 1.5523485905067638 2316 88-89 1.5993944284306245 2316 90-91 1.7713368788208221 2316 92-93 1.986380074065913 2316 94-95 2.5058077815035418 2316 96-97 2.96345218449002 2316 98-99 3.1986573328978345 2316 100-101 3.482828589515819 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 4.0 4 13.0 5 42.0 6 63.0 7 230.0 8 553.0 9 1133.0 10 2000.0 11 3288.0 12 5107.0 13 7624.0 14 10345.0 15 14436.0 16 18521.0 17 25793.0 18 26684.0 19 21714.0 20 20313.0 21 21454.0 22 22960.0 23 25089.0 24 28075.0 25 31982.0 26 36309.0 27 42743.0 28 51251.0 29 61575.0 30 73862.0 31 89522.0 32 111533.0 33 143051.0 34 193571.0 35 271139.0 36 379385.0 37 454801.0 38 237596.0 39 10717.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.79932855455535 18.954955633133533 15.3387154248758 22.907000387435314 2 15.654844757142284 19.883180189402587 40.1770626969534 24.284912356501724 3 19.39347448092462 23.48241791960724 29.97712999897719 27.146977600490946 4 13.027822731742916 17.018780303017905 36.632145294124385 33.321251671114794 5 13.777093962828541 37.1348062718249 34.32117411808599 14.766925647260576 6 32.459197065003245 36.21188889688893 16.919758864226946 14.409155173880887 7 28.5907257091289 32.72121900872088 20.088910597681796 18.59914468446842 8 26.396842188802683 35.06310958822292 19.23314507228128 19.306903150693113 9 25.83164994735072 16.94492648328191 20.17694575283557 37.04647781653179 10-11 24.9964818664762 26.619630039624003 26.900732999028833 21.48315509487097 12-13 26.040410209795102 23.730264784830492 27.442726021101215 22.78659898427319 14-15 22.581385473708497 25.296770926144557 25.93827802909251 26.183565571054434 16-17 22.04602421280563 28.48938127784671 26.897237737219914 22.56735677212774 18-19 22.046506452502335 26.727280834599455 29.399957782397713 21.8262549305005 20-21 22.43202843306424 25.893565006516727 29.911171219376897 21.763235341042137 22-23 22.22842668250645 25.846356563650808 29.796156889119068 22.129059864723676 24-25 22.173016761993438 26.000460644927443 29.78034682553871 22.046175767540404 26-27 21.68647758224861 26.326736696013093 29.65051883717952 22.33626688455878 28-29 21.86994364497356 26.77344461149806 29.437165679686622 21.919446063841754 30-31 21.973784224060978 26.2873475191928 29.39104633191049 22.347821924835724 32-33 21.652823220335794 26.315638758049776 29.397544179166267 22.633993842448163 34-35 22.45698370991019 26.44759034111307 29.130714495387693 21.964711453589047 36-37 22.516315985728536 25.982339237913017 29.20659115300996 22.294753623348484 38-39 21.999903453303514 26.441399223453647 29.098110671150906 22.460586652091934 40-41 22.490798974075126 26.217130098098597 29.168331166160105 22.123739761666172 42-43 22.15962343361549 26.252790558676292 28.967747953181206 22.619838054527015 44-45 22.174652420680406 26.498786243934287 29.17735401995845 22.149207315426853 46-47 22.656869061864 26.725881835567968 28.35312285869154 22.264126243876497 48-49 22.33752601034052 26.830828315695836 28.29493460827041 22.536711065693225 50-51 22.516089481305354 27.010875779569965 28.270788072132095 22.202246666992583 52-53 22.82992599465774 26.768074727844528 28.129762388397744 22.272236889099993 54-55 22.356407094692727 26.96055649333594 28.278619780011176 22.404416631960157 56-57 22.422696071983196 27.125199539047667 28.01971137902937 22.432393009939773 58-59 22.382177502759447 26.782725402562118 28.029187223991382 22.805909870687056 60-61 22.128563164737482 26.993384023636203 28.28910818504115 22.588944626585164 62-63 22.189633898966697 26.9753164591923 28.410059671684007 22.424989970156997 64-65 21.946693612354043 27.0743190727552 28.204568996187902 22.774418318702857 66-67 21.891794029380538 27.092693325041374 28.13572081738529 22.879791828192797 68-69 22.43026661605998 26.79398817154029 28.274561036468526 22.5011841759312 70-71 22.31571427519314 26.880806429307324 27.87480902662703 22.928670268872505 72-73 22.252705105919386 26.99648455893754 27.833861556342583 22.916948778800496 74-75 22.2391385155973 27.427226126358168 27.612041518612163 22.721593839432373 76-77 22.33307630443869 26.9827760634562 27.92082582585962 22.763321806245497 78-79 22.354238331588448 27.169650498535177 27.915882968782956 22.560228201093416 80-81 22.614574400534167 27.17729819783845 27.611452694077677 22.596674707549703 82-83 22.508300311468854 27.26269171131081 27.69795419267526 22.531053784545072 84-85 22.443734205805715 27.418066594215233 27.625477917588675 22.512721282390373 86-87 22.59998674127564 27.363797298657676 27.405189890304516 22.631026069762168 88-89 22.470520503686316 27.553191576421643 27.12338710799369 22.85290081189835 90-91 22.388551966865997 27.48767428732655 27.318714275279603 22.80505947052785 92-93 22.472086661318567 27.17706446588458 27.477818376145756 22.873030496651094 94-95 22.29146400964775 26.999448670621103 27.579111674374023 23.12997564535712 96-97 22.475637536043763 26.7145988619428 27.566740416373868 23.243023185639572 98-99 22.2047839435458 27.01955587486335 27.23519091914821 23.540469262442638 100-101 22.766431299900027 26.271362055347776 27.36924706841647 23.59295957633573 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 931.0 1 613.5 2 444.0 3 592.0 4 495.0 5 454.0 6 880.0 7 2093.0 8 3388.5 9 3863.5 10 3226.0 11 2354.5 12 2133.5 13 2243.0 14 2518.0 15 2782.0 16 2912.5 17 3064.5 18 3384.5 19 3749.5 20 4194.0 21 5030.0 22 6191.5 23 7677.5 24 9665.5 25 12314.0 26 15467.5 27 19221.0 28 22987.5 29 26395.5 30 29886.0 31 34883.5 32 42003.0 33 45149.0 34 45451.5 35 48346.0 36 52940.0 37 57533.0 38 61382.5 39 64421.0 40 68021.5 41 72821.5 42 75339.5 43 77979.0 44 82174.0 45 86578.0 46 88492.0 47 89722.5 48 90950.5 49 89173.0 50 86931.0 51 85203.5 52 84141.0 53 84648.5 54 85576.0 55 81432.5 56 74950.5 57 68278.0 58 63248.0 59 56788.5 60 45376.0 61 37069.5 62 31179.5 63 23143.0 64 16165.0 65 12018.5 66 8816.5 67 6697.5 68 5153.0 69 3926.0 70 2927.0 71 1995.5 72 1391.0 73 1055.5 74 780.0 75 553.0 76 368.0 77 215.5 78 144.5 79 96.0 80 61.5 81 41.0 82 22.0 83 15.5 84 10.0 85 5.0 86 2.0 87 1.0 88 1.5 89 1.0 90 0.5 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.008140797339963788 2 0.015217972916917231 3 0.009327144691013787 4 8.181705869310339E-5 5 8.999876456241374E-4 6 0.0018817923499413781 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 8.795333809508614E-4 14-15 0.0 16-17 0.0028226885249120672 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.003231773818377584 26-27 0.018613380852681022 28-29 0.00625900499002241 30-31 0.002945414112951722 32-33 0.0 34-35 0.008202160133983616 36-37 7.977163222577581E-4 38-39 0.0026590544075258604 40-41 0.002024972202654309 42-43 0.0030681397009913775 44-45 0.0 46-47 1.227255880396551E-4 48-49 7.158992635646547E-4 50-51 0.004152215728674997 52-53 0.00797716322257758 54-55 0.007036267047606891 56-57 0.016670225708719816 58-59 0.024565571872604292 60-61 0.0037635846998827563 62-63 0.02133379805422671 64-65 0.022479236875930158 66-67 0.014031625565867232 68-69 0.03254273509518187 70-71 0.017181582325551715 72-73 0.004868114992239652 74-75 0.017079311002185335 76-77 0.021456523642266363 78-79 0.027347351868169813 80-81 0.012354375862658614 82-83 0.036613133765163765 84-85 0.022172422905831022 86-87 0.03289045759462756 88-89 0.023563312903613777 90-91 0.02882005892464567 92-93 0.02534283393018878 94-95 0.015770238063095677 96-97 0.016383866003293956 98-99 0.012354375862658614 100-101 0.011393025423014647 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2444478.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.03988224004567 #Duplication Level Percentage of deduplicated Percentage of total 1 75.55795269209642 41.58700818471929 2 14.011828765073659 15.424188103946776 3 4.549829704002933 7.512662733617499 4 2.0954837711706342 4.613407200046341 5 1.0233186940323538 2.8161670206789036 6 0.6280839367780944 2.074179955027837 7 0.4118507349963731 1.5867751168273645 8 0.28921500309386367 1.273468776987256 9 0.21813726096394448 1.0805624240059455 >10 1.096465862401327 10.840557062420556 >50 0.07499559728225748 2.789856765189528 >100 0.036811218783768605 3.7823415253464603 >500 0.0033481996247651975 1.3034238848390511 >1k 0.002455346391492716 2.292622198847122 >5k 1.4880887221167976E-4 0.5997027576213718 >10k+ 7.440443610583988E-5 0.42307628987888496 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10331 0.4226260166792256 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8858 0.36236775295175494 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5786 0.23669675079914812 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2912 0.11912563745715854 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2870 0.11740747922460337 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2840 0.11618022334420681 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2734 0.11184391923347234 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2656 0.1086530539444413 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.227255880396551E-4 2 0.0 0.0 0.0 0.0 1.227255880396551E-4 3 0.0 0.0 0.0 0.0 1.227255880396551E-4 4 0.0 0.0 0.0 0.0 1.227255880396551E-4 5 0.0 0.0 0.0 4.0908529346551695E-5 2.045426467327585E-4 6 0.0 0.0 0.0 4.0908529346551695E-5 2.045426467327585E-4 7 0.0 0.0 0.0 4.0908529346551695E-5 2.045426467327585E-4 8 0.0 0.0 0.0 4.0908529346551695E-5 2.045426467327585E-4 9 0.0 0.0 0.0 4.0908529346551695E-5 2.045426467327585E-4 10-11 0.0 0.0 0.0 1.0227132336637924E-4 2.045426467327585E-4 12-13 0.0 0.0 0.0 1.227255880396551E-4 6.545364695448271E-4 14-15 0.0 0.0 0.0 1.227255880396551E-4 7.363535282379305E-4 16-17 0.0 0.0 0.0 1.227255880396551E-4 8.18170586931034E-4 18-19 0.0 0.0 0.0 1.227255880396551E-4 8.999876456241374E-4 20-21 0.0 0.0 0.0 2.045426467327585E-4 9.613504396439649E-4 22-23 0.0 0.0 0.0 2.6590544075258605E-4 0.0010636217630103442 24-25 0.0 0.0 0.0 5.727194108517237E-4 0.001206801615723275 26-27 0.0 0.0 0.0 0.0011249845570301717 0.0013090729390896542 28-29 0.0 0.0 0.0 0.003129502495011205 0.0013090729390896542 30-31 0.0 0.0 0.0 0.010043043954578442 0.0013090729390896542 32-33 0.0 0.0 0.0 0.024156486579138776 0.0013090729390896542 34-35 0.0 0.0 0.0 0.045817552868137906 0.001349981468436206 36-37 0.0 0.0 0.0 0.08089661678280598 0.0013704357331094817 38-39 0.0 0.0 0.0 0.13086638537961887 0.001472707056475861 40-41 0.0 0.0 0.0 0.18494746117576022 0.001472707056475861 42-43 2.0454264673275847E-5 0.0 0.0 0.23908989976592138 0.001493161321149137 44-45 4.0908529346551695E-5 0.0 0.0 0.3071616925985834 0.0015340698504956885 46-47 4.0908529346551695E-5 0.0 0.0 0.3864833310015472 0.0015545241151689645 48-49 4.0908529346551695E-5 0.0 0.0 0.47024354483861175 0.0015545241151689645 50-51 4.0908529346551695E-5 0.0 0.0 0.5590355077853022 0.0015954326445155162 52-53 4.0908529346551695E-5 0.0 0.0 0.6457411357353185 0.001636341173862068 54-55 4.0908529346551695E-5 0.0 0.0 0.739053491174803 0.001636341173862068 56-57 4.0908529346551695E-5 0.0 0.0 0.8500996940860175 0.0016567954385353439 58-59 4.0908529346551695E-5 0.0 0.0 0.9668730911057494 0.0016772497032086196 60-61 4.0908529346551695E-5 0.0 0.0 1.0904577582616821 0.0016772497032086196 62-63 4.0908529346551695E-5 0.0 0.0 1.2108311058639103 0.0017999752912482747 64-65 4.0908529346551695E-5 0.0 0.0 1.3347225869899422 0.0017999752912482747 66-67 4.0908529346551695E-5 0.0 0.0 1.475366110883387 0.0017999752912482747 68-69 4.0908529346551695E-5 0.0 0.0 1.632495772103492 0.0017999752912482747 70-71 4.0908529346551695E-5 0.0 0.0 1.7964162491951248 0.0017999752912482747 72-73 4.0908529346551695E-5 0.0 0.0 1.9629753264296097 0.0019227008792879299 74-75 4.0908529346551695E-5 4.0908529346551695E-5 0.0 2.1309252936618783 0.002065880732000861 76-77 4.0908529346551695E-5 4.0908529346551695E-5 0.0 2.3130909748420727 0.0020863349966741365 78-79 4.0908529346551695E-5 4.0908529346551695E-5 0.0 2.522972184654556 0.0021067892613474124 80-81 4.0908529346551695E-5 4.0908529346551695E-5 0.0 2.7414646398944886 0.002188606320040516 82-83 4.0908529346551695E-5 4.0908529346551695E-5 0.0 2.9634138658642053 0.0022908776434068952 84-85 6.136279401982754E-5 4.0908529346551695E-5 0.0 3.187797149330041 0.0022908776434068952 86-87 8.181705869310339E-5 4.0908529346551695E-5 0.0 3.42418708615909 0.0023726947020999986 88-89 8.181705869310339E-5 4.0908529346551695E-5 0.0 3.676531349433294 0.0024749660254663776 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5035 0.0 54.629322 1 GTATCAA 8610 0.0 39.78129 1 ATCAACG 11155 0.0 30.698328 3 TCAACGC 11265 0.0 30.353918 4 TATCAAC 11245 0.0 30.330883 2 CAACGCA 11405 0.0 29.981316 5 GTGGTAT 2385 0.0 29.87787 1 AACGCAG 11855 0.0 28.803205 6 TGGTATC 2360 0.0 28.58225 2 ACGCAGA 13325 0.0 25.445898 7 CGCAGAG 13395 0.0 25.206566 8 TAAGGTG 1590 0.0 23.297586 5 GCAGAGT 14800 0.0 22.428606 9 GAGTACT 10080 0.0 21.719257 12-13 GTACATG 7860 0.0 21.274866 1 GTACTTT 10265 0.0 21.212162 14-15 TACATGG 7660 0.0 20.712751 2 GTAAGGT 1630 0.0 20.686365 4 CAGAGTA 14430 0.0 20.404688 10-11 AGAGTAC 13400 0.0 20.325123 10-11 >>END_MODULE