FastQCFastQC Report
Thu 26 May 2016
SRR937999_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937999_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2397308
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117150.488673128358976No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93870.3915642045160655No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59960.25011387773285704No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA47970.2000994448773374No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC45230.1886699581363763No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA44290.18474889334203198No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA43170.18007698635302596No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA39970.16672868067015167No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG36690.15304666734520556No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA34800.14516282430125793No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA32650.13619443142057674No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA31650.13202308589467854No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT31220.1302294073185423No Hit
GGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCT26850.1120006273703671No Hit
GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA25810.10766242802343294No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA25630.10691158582877126No Hit
CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG24170.10082142136095988No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58100.050.408261
GTATCAA101850.040.3697171
TATCAAC128050.032.1375662
ATCAACG128800.031.8028533
TCAACGC132300.030.8896714
CAACGCA132800.030.7018035
AACGCAG136250.029.9530246
GTGTAAG11250.027.5594331
ACGCAGA150750.026.846327
CGCAGAG151050.026.7938
TAAGGTG20700.025.9407655
TAAGGTA11300.025.2317564
AGGTAAG18600.025.037322
AAGGTAA18650.024.8086051
GCAGAGT163200.024.0696479
GAGTACT111350.022.71552712-13
GGTAAGG22700.022.1898673
GTAAGGT23450.022.08814
AGAGTAC142300.021.9806110-11
ATACGGC1302.938658E-421.9276436
Produced by FastQC (version 0.11.5)