FastQCFastQC Report
Thu 26 May 2016
SRR937999_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937999_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2397308
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112270.46831696219259267No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92660.3865168764297287No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60600.2527835388694319No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC50940.2124883410892551No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA48610.20276910601391226No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA43520.18153695728709035No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA43010.17940957106888225No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA42720.17819988086637178No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG38180.1592619721787939No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA35500.14808276616938668No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA32960.1374875485336052No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA31800.13264878772356326No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT30320.1264751963452339No Hit
GGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCT27920.11646396708307818No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA26900.11220919464666201No Hit
GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA24760.10328251522123982No Hit
TCAGTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAG24200.10094656172673683No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63050.045.784821
GTATCAA105800.038.5223771
TATCAAC133700.030.4125392
ATCAACG132850.030.3885273
TCAACGC134100.030.0685484
CAACGCA135300.029.8370085
AACGCAG139000.029.1113686
TAAGGTG21100.027.718395
ACGCAGA153950.026.099287
CGCAGAG155100.025.9363848
AAGGTAA19850.024.1979641
GTAAGGT22700.024.0889234
GTGTAAG10900.023.9968431
TACCGTA1000.00165540523.7469487
AGGTAAG21550.023.613182
GCAGAGT168900.023.3392169
GAGTACT113100.023.15905212-13
TAAGGTA11400.022.9405174
ATACCGT1703.154868E-622.350076
GGTAAGG23500.022.0557673
Produced by FastQC (version 0.11.5)