FastQCFastQC Report
Thu 26 May 2016
SRR937998_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937998_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2353024
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117050.4974449899363543No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93730.3983384784855573No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58870.25018869335799376No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA46420.1972780558124354No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC44360.18852336397758798No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA42810.1819360958494261No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA41770.17751625142794972No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA38750.1646817032040472No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG37110.15771194853941142No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA34370.1460673584289833No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA31710.13476275635097643No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA29840.1268155360931295No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT29790.12660304357286623No Hit
GGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCT25320.1076062122613284No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA25180.1070112332045912No Hit
GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA25090.10662874666811728No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60850.047.3578761
GTATCAA102600.039.851931
TATCAAC128150.031.74582
ATCAACG128250.031.6476043
TCAACGC130650.031.0662524
CAACGCA131650.030.7219725
AACGCAG134850.030.0249866
ACGCAGA149650.026.9205537
CGCAGAG150900.026.571628
TAAGGTG20850.025.7580385
AGGTAAG18350.025.6407222
TAAGGTA10100.024.9399094
AAGGTAA17700.023.9901311
GGTAAGG21000.023.9898173
GCAGAGT164650.023.9466239
GTGTAAG11850.023.75471
GAGTACT111250.023.2290512-13
GTAAGGT22150.022.9588644
GTACATG71250.022.3654421
TACATGG69400.022.1879252
Produced by FastQC (version 0.11.5)