##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937998_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2353024 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.864086809144318 33.0 31.0 34.0 30.0 34.0 2 32.05991694092368 34.0 31.0 34.0 30.0 34.0 3 32.13483032897242 34.0 31.0 34.0 30.0 34.0 4 35.667825317548825 37.0 35.0 37.0 35.0 37.0 5 35.67427191562857 37.0 35.0 37.0 35.0 37.0 6 35.70388997307295 37.0 35.0 37.0 35.0 37.0 7 35.65035588247294 37.0 35.0 37.0 33.0 37.0 8 35.617691532258064 37.0 35.0 37.0 33.0 37.0 9 37.25511299502258 39.0 37.0 39.0 34.0 39.0 10-11 37.16597557015993 39.0 37.0 39.0 34.0 39.0 12-13 37.066166558437146 39.0 37.0 39.0 33.0 39.0 14-15 38.242117164975795 40.0 38.0 41.0 33.0 41.0 16-17 38.09137305866834 40.0 38.0 41.0 33.0 41.0 18-19 38.24969911059131 40.0 38.0 41.0 33.5 41.0 20-21 38.22606059266714 40.0 38.0 41.0 34.0 41.0 22-23 38.13995862345645 40.0 38.0 41.0 33.5 41.0 24-25 38.02114024336343 40.0 38.0 41.0 33.0 41.0 26-27 37.8365320540717 40.0 38.0 41.0 33.0 41.0 28-29 37.61978522106022 40.0 37.5 41.0 32.0 41.0 30-31 37.3697860710303 40.0 37.0 41.0 32.0 41.0 32-33 37.07827799461459 40.0 36.5 41.0 31.0 41.0 34-35 36.82093701551705 39.5 36.0 41.0 30.5 41.0 36-37 36.52336546503563 39.0 35.5 41.0 30.0 41.0 38-39 36.1969567671218 39.0 35.0 40.5 30.0 41.0 40-41 35.950614825858125 39.0 35.0 40.0 29.0 41.0 42-43 35.73794444935538 39.0 35.0 40.0 27.5 41.0 44-45 35.445517767774575 39.0 35.0 40.0 26.5 41.0 46-47 35.0315047785318 38.0 34.5 40.0 25.0 41.0 48-49 34.593598067848006 38.0 34.0 40.0 23.5 41.0 50-51 33.87338951918892 37.5 33.5 39.5 22.5 40.5 52-53 33.978822145460484 38.0 33.5 40.0 23.0 40.5 54-55 34.06228410759941 38.0 34.0 40.0 22.5 41.0 56-57 33.73137651804656 38.0 33.0 40.0 20.0 41.0 58-59 33.391117770154494 37.5 33.0 40.0 18.5 41.0 60-61 32.94567841211989 37.0 32.5 40.0 15.5 41.0 62-63 32.394339794239244 36.0 31.5 39.0 10.0 41.0 64-65 31.856218636104007 35.5 31.0 39.0 7.5 40.0 66-67 31.306047664622206 35.0 31.0 38.5 4.0 40.0 68-69 30.68501341252788 35.0 30.0 38.0 2.0 40.0 70-71 30.097255489106782 35.0 29.0 37.0 2.0 39.5 72-73 29.43593499258826 34.0 28.5 36.5 2.0 39.0 74-75 28.73464104063537 34.0 27.5 36.0 2.0 39.0 76-77 28.047308909726375 34.0 26.0 35.5 2.0 38.0 78-79 27.2937269233123 33.0 25.5 35.0 2.0 37.0 80-81 26.61437048665887 32.5 24.5 35.0 2.0 36.5 82-83 25.85748062918185 32.0 21.0 35.0 2.0 36.0 84-85 25.16921863100419 31.5 19.0 34.5 2.0 35.5 86-87 24.46141348324539 31.0 14.5 34.0 2.0 35.0 88-89 23.598379149553935 30.5 6.0 34.0 2.0 35.0 90-91 22.735832911181525 30.0 2.0 34.0 2.0 35.0 92-93 21.822993093143122 29.0 2.0 34.0 2.0 35.0 94-95 20.83011945479519 29.0 2.0 34.0 2.0 35.0 96-97 19.594015615650328 26.5 2.0 33.5 2.0 35.0 98-99 18.06224352152804 24.5 2.0 33.0 2.0 34.5 100-101 14.47716045395202 2.0 2.0 29.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.43496207210600346 1101 2 0.3961422043300331 1101 3 0.3634745662375849 1101 4 0.27472219710303136 1101 5 0.35796766477206887 1101 6 0.34329498841258044 1101 7 0.2297022986853392 1101 8 0.2386194876387009 1101 9 0.34548753275191046 1101 10-11 0.4740166046153007 1101 12-13 0.45990545810757766 1101 14-15 0.6237666889338058 1101 16-17 0.7653811005606457 1101 18-19 0.6447000587974898 1101 20-21 0.7542556348798115 1101 22-23 0.6801694129364932 1101 24-25 0.6086613736569575 1101 26-27 0.7759430609691336 1101 28-29 0.8468692489960432 1101 30-31 0.8271739402813054 1101 32-33 0.8224303291265542 1101 34-35 0.956782962307976 1101 36-37 0.9642596093947802 1101 38-39 0.9372435494319262 1101 40-41 0.9762198594395386 1101 42-43 1.2258138629192032 1101 44-45 1.1865171151438076 1101 46-47 1.2856226441825598 1101 48-49 1.3251873641267409 1101 50-51 1.2946048860422295 1101 52-53 1.275623749297253 1101 54-55 1.3165525474175297 1101 56-57 1.4220621377746667 1101 58-59 1.4157931536132224 1101 60-61 1.4348650144794206 1101 62-63 1.4254620464758219 1101 64-65 1.4642957065313844 1101 66-67 1.4717099569500043 1101 68-69 1.7251064350752898 1101 70-71 1.9427652240269886 1101 72-73 2.1655425216176916 1101 74-75 2.269277620512103 1101 76-77 2.436237410025086 1101 78-79 2.6011325349284355 1101 80-81 2.853769925907706 1101 82-83 2.9756020484888914 1101 84-85 3.0235608521227952 1101 86-87 3.4431333276205685 1101 88-89 3.827875216565186 1101 90-91 4.057128038430719 1101 92-93 4.354526683872141 1101 94-95 4.5914212586027645 1101 96-97 4.65954169325104 1101 98-99 5.001570634590774 1101 100-101 4.913863852082358 1102 1 0.46538751459421235 1102 2 0.33098112845803485 1102 3 0.2800659167365893 1102 4 0.30317247881754383 1102 5 0.3251179115984897 1102 6 0.3497657689444722 1102 7 0.11636955139878324 1102 8 0.3611000588145359 1102 9 0.42631940783603994 1102 10-11 0.4775040407796993 1102 12-13 0.4960270005460643 1102 14-15 0.6493222200707507 1102 16-17 0.6797335692369018 1102 18-19 0.5414573887317502 1102 20-21 0.6217928099328986 1102 22-23 0.6815724913303214 1102 24-25 0.5865410838934508 1102 26-27 0.5953174926640088 1102 28-29 0.604757530166637 1102 30-31 0.720800956305105 1102 32-33 0.6545814962295466 1102 34-35 0.6803044074993139 1102 36-37 0.7704960627862079 1102 38-39 0.6105192670416244 1102 40-41 0.7965674492763881 1102 42-43 0.8927465398801431 1102 44-45 0.8142432628111109 1102 46-47 0.8017563287776781 1102 48-49 1.0668204017811647 1102 50-51 1.0325829147823313 1102 52-53 0.9480760254022016 1102 54-55 1.0753267664873079 1102 56-57 1.104348324560597 1102 58-59 1.1694536567160654 1102 60-61 1.1902973933806322 1102 62-63 1.1547441483325898 1102 64-65 1.3582922067332994 1102 66-67 1.2650261392581719 1102 68-69 1.5303910203052737 1102 70-71 1.5761915922859622 1102 72-73 1.8565301294754804 1102 74-75 1.7582343015827 1102 76-77 1.9512022060121268 1102 78-79 2.2490196073775515 1102 80-81 2.2795887660295655 1102 82-83 2.5414038840972744 1102 84-85 2.7547714141508095 1102 86-87 2.761033943244392 1102 88-89 3.1843821213866157 1102 90-91 3.4713177417720082 1102 92-93 3.7909575491244176 1102 94-95 3.7754518502444547 1102 96-97 3.9374377486983896 1102 98-99 3.922642495829546 1102 100-101 3.9215893679179814 1103 1 0.5081914081808705 1103 2 0.3975970934663735 1103 3 0.4079927159041503 1103 4 0.2589831018590658 1103 5 0.3160930999442897 1103 6 0.280868229311352 1103 7 0.40323301901633357 1103 8 0.3299127754920832 1103 9 0.335364892736429 1103 10-11 0.47763371841487157 1103 12-13 0.5431895661836208 1103 14-15 0.8041846302204334 1103 16-17 0.6424668480262739 1103 18-19 0.6091751751157162 1103 20-21 0.6737409188320385 1103 22-23 0.6589808931881578 1103 24-25 0.7949692531334165 1103 26-27 0.7510030738891942 1103 28-29 0.9335740306746985 1103 30-31 0.9421521596759774 1103 32-33 0.9935381613467698 1103 34-35 1.1145497024564222 1103 36-37 1.2132354307862698 1103 38-39 1.13043186306259 1103 40-41 1.2114213646012786 1103 42-43 1.3167803788518455 1103 44-45 1.365616029004208 1103 46-47 1.2109266049096021 1103 48-49 1.3468794896775407 1103 50-51 1.4150087761362684 1103 52-53 1.3264341436606841 1103 54-55 1.400058734718172 1103 56-57 1.3845959775206964 1103 58-59 1.3493902086441238 1103 60-61 1.3245497047122043 1103 62-63 1.4972191493662521 1103 64-65 1.6154526048123152 1103 66-67 1.8346094042027268 1103 68-69 1.9645648985937214 1103 70-71 2.160890508381403 1103 72-73 2.324592469962589 1103 74-75 2.33854622954458 1103 76-77 2.6132902289356252 1103 78-79 2.7900652726989996 1103 80-81 2.9351411614323375 1103 82-83 3.1612420564319628 1103 84-85 3.287898975597333 1103 86-87 3.390390328708971 1103 88-89 3.6772582460425305 1103 90-91 3.925590400589524 1103 92-93 4.323841040973523 1103 94-95 4.701614782988745 1103 96-97 4.944856311769957 1103 98-99 4.900618722849355 1103 100-101 4.72765953416847 1104 1 0.36625487908386845 1104 2 0.3613112429599781 1104 3 0.3383888072963259 1104 4 0.27146944672751516 1104 5 0.3483377258399756 1104 6 0.3378855782717096 1104 7 0.3232132537920194 1104 8 0.2499490194514209 1104 9 0.43270792815644654 1104 10-11 0.3566151150339536 1104 12-13 0.3630413501455507 1104 14-15 0.5190470837004071 1104 16-17 0.6624738144577975 1104 18-19 0.5136750038479718 1104 20-21 0.48548236470217887 1104 22-23 0.5088014092132624 1104 24-25 0.5606215055357922 1104 26-27 0.7188401473399111 1104 28-29 0.6158099774613461 1104 30-31 0.549670003924156 1104 32-33 0.635005902834294 1104 34-35 0.6454270793750894 1104 36-37 0.7135430184105616 1104 38-39 0.8927536520236927 1104 40-41 1.053212431830815 1104 42-43 0.8525265044975754 1104 44-45 0.9319962850726498 1104 46-47 0.9770490335789006 1104 48-49 1.0650604872890455 1104 50-51 0.9998018198882335 1104 52-53 0.9462546828300873 1104 54-55 1.1234602742763897 1104 56-57 1.3744967348018875 1104 58-59 1.3275357492021769 1104 60-61 1.3060908598900411 1104 62-63 1.4071337006447422 1104 64-65 1.631123488368761 1104 66-67 1.6387530423000847 1104 68-69 1.7017753635162336 1104 70-71 1.7318549541219248 1104 72-73 2.0114901397953986 1104 74-75 2.2872529265765564 1104 76-77 2.396179539050607 1104 78-79 2.603921875302266 1104 80-81 2.622071811879483 1104 82-83 2.757489441127106 1104 84-85 2.869955206966182 1104 86-87 3.080418495310834 1104 88-89 3.3195914682832672 1104 90-91 3.414865336248397 1104 92-93 3.6554088120857458 1104 94-95 3.9762587080753065 1104 96-97 4.271128900597748 1104 98-99 4.337963887544131 1104 100-101 4.359923254104036 1105 1 0.2955905402889911 1105 2 0.2464900861867676 1105 3 0.1624945911623783 1105 4 0.15974279259554436 1105 5 0.10337398691974897 1105 6 0.20589077121646682 1105 7 0.18763150967884457 1105 8 0.12832331437487454 1105 9 0.2386887679809604 1105 10-11 0.2007232491551889 1105 12-13 0.11916320528030155 1105 14-15 0.18941540226071396 1105 16-17 0.33940046411309055 1105 18-19 0.25751677571653175 1105 20-21 0.3215604603239868 1105 22-23 0.3538944121678753 1105 24-25 0.3312533884259352 1105 26-27 0.3532467638678014 1105 28-29 0.5123735686512418 1105 30-31 0.4759811105585854 1105 32-33 0.5660175198098543 1105 34-35 0.5321307905399166 1105 36-37 0.5683140364068038 1105 38-39 0.6977776022430433 1105 40-41 0.8787031874938123 1105 42-43 0.8544906463186805 1105 44-45 0.9762230511215293 1105 46-47 0.9360510704667178 1105 48-49 0.9669373339181107 1105 50-51 1.0435445452889027 1105 52-53 1.0061134436860257 1105 54-55 1.1445817936604001 1105 56-57 1.1777020351868828 1105 58-59 1.220358517578937 1105 60-61 1.3743685705176745 1105 62-63 1.3436880027308717 1105 64-65 1.406038991099532 1105 66-67 1.5418917521219164 1105 68-69 1.526714928383285 1105 70-71 1.7337379561811943 1105 72-73 1.8094437390433171 1105 74-75 2.0330672340347 1105 76-77 2.244109635746817 1105 78-79 2.367707241046741 1105 80-81 2.48741897814325 1105 82-83 2.6332728595172945 1105 84-85 2.6112144960699517 1105 86-87 2.8258494873423494 1105 88-89 3.025708846741498 1105 90-91 3.186868201321367 1105 92-93 3.3948802285061923 1105 94-95 3.7850290769534496 1105 96-97 3.9285148572635187 1105 98-99 4.225923866056082 1105 100-101 4.0642359723420185 1106 1 0.1416913333915133 1106 2 0.19059566733028532 1106 3 0.07636458003185709 1106 4 0.18668291220237876 1106 5 0.12908315581365315 1106 6 -0.009782186939972348 1106 7 0.15411684658745628 1106 8 0.18465424979023481 1106 9 0.086934955525237 1106 10-11 0.20511098758692725 1106 12-13 0.2730149929682568 1106 14-15 0.4808116413977288 1106 16-17 0.20294438095928058 1106 18-19 0.32262424355997865 1106 20-21 0.16278056632864946 1106 22-23 0.20456198369699052 1106 24-25 0.11092587758691508 1106 26-27 0.1264047955288916 1106 28-29 0.273015770903271 1106 30-31 0.2822545583021352 1106 32-33 0.28797360442521125 1106 34-35 0.3037462363405723 1106 36-37 0.37186834975229033 1106 38-39 0.34527269873951383 1106 40-41 0.6216934266067966 1106 42-43 0.6580529169972777 1106 44-45 0.7591584528418096 1106 46-47 0.9308155512702214 1106 48-49 1.011916890067809 1106 50-51 0.8645656919828184 1106 52-53 0.8275678590477682 1106 54-55 0.5209396963152599 1106 56-57 0.7689751630131596 1106 58-59 0.953191220983804 1106 60-61 1.0885416525811635 1106 62-63 0.9479962165499316 1106 64-65 1.119793014018601 1106 66-67 1.3354832408285127 1106 68-69 1.162654515660769 1106 70-71 1.203471346547495 1106 72-73 1.644918163362746 1106 74-75 1.6827370371897672 1106 76-77 1.82102676028493 1106 78-79 2.158401854516846 1106 80-81 2.4044546864319685 1106 82-83 2.3922597740889877 1106 84-85 2.5875688747630043 1106 86-87 2.465044841818802 1106 88-89 2.813572192374597 1106 90-91 3.00770226949005 1106 92-93 3.232561594803027 1106 94-95 3.2584995719742693 1106 96-97 3.4781238761373388 1106 98-99 3.782498230906622 1106 100-101 3.6799488308401713 1107 1 0.15377742670418826 1107 2 0.15448643765184045 1107 3 0.06903585721529026 1107 4 0.1029331402884992 1107 5 0.06513071006363447 1107 6 0.13246181021771264 1107 7 0.05452114933897434 1107 8 0.06920948930694237 1107 9 0.10073826472341096 1107 10-11 0.1206998247579989 1107 12-13 0.14584797870070076 1107 14-15 0.26142629015138397 1107 16-17 0.4099089033889243 1107 18-19 0.33079368828813216 1107 20-21 0.3897441034576232 1107 22-23 0.4029238742755865 1107 24-25 0.4624453822254395 1107 26-27 0.3459125248831185 1107 28-29 0.48090707215753525 1107 30-31 0.5216390026953306 1107 32-33 0.41749474987174295 1107 34-35 0.34961653217710165 1107 36-37 0.45025725451372267 1107 38-39 0.4428635915489991 1107 40-41 0.506490598229071 1107 42-43 0.7003429471509293 1107 44-45 0.6218993239448665 1107 46-47 0.6818942436883759 1107 48-49 0.8720746417246659 1107 50-51 0.6617027973546072 1107 52-53 0.6265995501139656 1107 54-55 0.790076455262863 1107 56-57 0.8302785934127215 1107 58-59 0.9649126118455129 1107 60-61 1.049682994745801 1107 62-63 0.973265648428395 1107 64-65 1.2537756259722137 1107 66-67 1.4804507000189382 1107 68-69 1.49609908755626 1107 70-71 1.532670182574794 1107 72-73 1.778440210014221 1107 74-75 1.967953181247001 1107 76-77 2.059667993015296 1107 78-79 2.052775269975303 1107 80-81 2.1974592556853487 1107 82-83 2.3673917580938557 1107 84-85 2.5027428815273787 1107 86-87 2.7595203017918273 1107 88-89 2.974934395644947 1107 90-91 3.049822225452761 1107 92-93 3.2792671996732956 1107 94-95 3.4852291786431877 1107 96-97 3.437873402667421 1107 98-99 3.7105844616593338 1107 100-101 3.710295385245324 1108 1 0.1607679891268532 1108 2 0.14272225743061284 1108 3 0.11253080521092329 1108 4 0.012356631692291842 1108 5 0.0298209027683356 1108 6 0.03913808965239696 1108 7 0.09470908299270064 1108 8 0.06854881345462616 1108 9 0.16285466908368562 1108 10-11 0.10365470583785452 1108 12-13 0.10781958569398853 1108 14-15 0.24585193988412612 1108 16-17 0.3213785663715356 1108 18-19 0.29262057364527294 1108 20-21 0.2391078129359414 1108 22-23 0.24959497372078232 1108 24-25 0.32042256603781993 1108 26-27 0.31957099090950436 1108 28-29 0.41510090978758285 1108 30-31 0.3578607607716435 1108 32-33 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-1.286569618890855 2316 66-67 -1.358206075258753 2316 68-69 -1.42721445068943 2316 70-71 -1.798984028533745 2316 72-73 -1.7985921991563814 2316 74-75 -2.032577486627421 2316 76-77 -2.1964163355892623 2316 78-79 -2.89537810339856 2316 80-81 -2.6457783767883463 2316 82-83 -2.608402956454114 2316 84-85 -2.626621486444183 2316 86-87 -2.5108832024475873 2316 88-89 -2.7245706045674716 2316 90-91 -2.991810883538914 2316 92-93 -3.456415238462615 2316 94-95 -3.5038305814649107 2316 96-97 -3.8091933250273016 2316 98-99 -3.8651177500472755 2316 100-101 -3.598039312912743 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6762.0 3 1909.0 4 1973.0 5 2356.0 6 2616.0 7 3340.0 8 4220.0 9 5464.0 10 6976.0 11 8788.0 12 11556.0 13 14334.0 14 16980.0 15 20981.0 16 24926.0 17 30473.0 18 29217.0 19 25213.0 20 24826.0 21 25504.0 22 27793.0 23 31301.0 24 35036.0 25 39084.0 26 44112.0 27 51140.0 28 58673.0 29 68135.0 30 81284.0 31 97090.0 32 118134.0 33 148459.0 34 193040.0 35 256283.0 36 324187.0 37 339812.0 38 162289.0 39 8758.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.385702349240724 18.471232336291145 15.061347191760088 23.081718122708043 2 15.32303288979438 19.8323006955159 40.30812047445927 24.536545940230443 3 18.975608013756528 23.86646403793591 30.296978087862858 26.860949860444705 4 12.704757352809857 16.97794688576621 37.16852504968776 33.14877071173618 5 13.485242182352719 37.194898128885754 34.42248790864032 14.897371780121214 6 31.838574368196344 36.61824164251512 17.073393769623575 14.46979021966496 7 27.995726070544496 33.50259828719683 20.471832968101996 18.029842674156672 8 26.75650821171336 34.17049769415582 19.73655512364231 19.336438970488512 9 26.476189342380007 16.742695744936864 20.2804395159593 36.50067539672383 10-11 24.830034004891857 26.42472418195328 27.18033647728879 21.564905335866072 12-13 26.272384502810848 23.890118122656745 27.39327846283231 22.444218911700105 14-15 22.634621577928684 25.233976835618343 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22.646021464786553 40-41 22.44132796529222 26.035998352349747 29.503928047504697 22.018745634853328 42-43 22.12428465916035 25.988342003326302 29.248585259063642 22.63878807844971 44-45 21.993996163920666 26.318989655337305 29.539829873600652 22.147184307141377 46-47 22.755607739245544 26.78472895891555 28.462183309286704 21.997479992552204 48-49 22.28731230187627 26.818636096788744 28.280665419419726 22.61338618191526 50-51 22.500761914234598 27.06026072131281 28.329161401276103 22.109815963176484 52-53 23.016421203683198 26.545473170652915 28.33039123465706 22.107714391006827 54-55 22.187273393447207 26.890679685766578 28.563413280196 22.358633640590213 56-57 22.40372854952325 27.029327473293495 28.238066951946895 22.328877025236356 58-59 22.455046870606775 26.485627898663683 28.187480726934854 22.871844503794684 60-61 21.73744868491411 26.912313155279517 28.702847291235056 22.64739086857132 62-63 22.073097953604144 26.90026115520372 28.862601928739995 22.16403896245214 64-65 21.706753606124302 27.03848303681679 28.50445996867289 22.750303388386016 66-67 21.6409781035176 26.936056559041617 28.545246978278627 22.877718359162156 68-69 22.411829499238863 26.51899698584006 28.678935967324747 22.390237547596335 70-71 22.16755416653826 26.852660852488704 27.984140832468153 22.995644148504887 72-73 22.091457234528786 26.946447444511012 28.16084320180868 22.80125211915152 74-75 22.165344574833032 27.398499008165473 28.01302412408494 22.423132292916552 76-77 22.461621897083333 26.92252847822377 28.04557025832766 22.57027936636523 78-79 22.476162261117867 27.28158178959543 27.821456153989686 22.420799795297018 80-81 22.902060932147215 27.2376886768493 27.447073993225796 22.413176397777693 82-83 22.79111135669549 27.42536655239909 27.547717163302043 22.23580492760338 84-85 22.684178368585197 27.6346344910396 27.47966442988678 22.201522710488426 86-87 23.0875219486467 27.56144399198363 26.93233622426114 22.418697835108528 88-89 22.75432975769596 27.728728811920146 26.793856963310592 22.72308446707331 90-91 22.655245315039448 27.66827320607705 26.93727439259504 22.739207086288467 92-93 23.066886818056524 27.00740258694177 27.269025409293075 22.656685185708636 94-95 22.722161547222413 26.792131418506866 27.515484170201947 22.970222864068777 96-97 23.08061000141133 26.33851218421451 27.480500665706508 23.100377148667654 98-99 22.71780876654913 26.78486183641414 26.93938060715037 23.557948789886353 100-101 23.616671217376147 25.59353772296969 27.28427349288695 23.50551756676721 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 697.0 1 501.5 2 452.0 3 595.0 4 491.0 5 469.5 6 848.0 7 2052.0 8 3525.0 9 4011.0 10 3220.5 11 2355.0 12 2218.5 13 2330.0 14 2585.0 15 2814.5 16 3049.5 17 3292.0 18 3585.5 19 4054.5 20 4623.5 21 5464.0 22 6683.5 23 8171.5 24 10268.5 25 13194.0 26 16677.0 27 20645.5 28 23834.0 29 26932.5 30 30856.0 31 36719.5 32 45085.5 33 47384.0 34 45174.0 35 46372.5 36 49699.5 37 53616.5 38 56853.5 39 59467.5 40 63185.0 41 68015.5 42 70765.5 43 72080.5 44 74118.5 45 79693.0 46 83221.0 47 84882.5 48 86446.5 49 83977.0 50 81263.0 51 79276.5 52 78088.5 53 79075.5 54 81823.0 55 78748.5 56 72216.5 57 66659.0 58 62439.5 59 55304.5 60 43410.0 61 36171.0 62 32050.5 63 24059.0 64 16063.0 65 12000.5 66 9141.0 67 7107.0 68 5538.0 69 4372.5 70 3393.5 71 2284.5 72 1608.5 73 1193.5 74 865.0 75 648.0 76 453.5 77 287.5 78 184.5 79 130.5 80 82.0 81 44.5 82 33.5 83 26.5 84 16.0 85 9.5 86 11.0 87 11.5 88 8.5 89 6.0 90 5.0 91 3.0 92 1.5 93 2.0 94 1.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.46166124952401677 2 0.04067106837839308 3 0.053888103138769516 4 0.056862998422455534 5 0.08350956046347169 6 0.08066216069194365 7 0.04632336941739651 8 0.04067106837839308 9 0.045600894848501335 10-11 0.04914951993689823 12-13 0.0451334113039221 14-15 0.03916237148452374 16-17 0.04018233558178752 18-19 0.04755582603492357 20-21 0.055545544796823144 22-23 0.04740708127073927 24-25 0.06449147989990753 26-27 0.055736788065060106 28-29 0.04504841429581679 30-31 0.04640836642550182 32-33 0.04485717102757983 34-35 0.0500207392699777 36-37 0.04517590980797476 38-39 0.026051582984278954 40-41 0.02477662786269923 42-43 0.0238629100255671 44-45 0.01536320921503563 46-47 0.027624027634227277 48-49 0.027326538105858673 50-51 0.016871906108904964 52-53 0.016786909100799653 54-55 0.016064434531904478 56-57 0.015575701735298918 58-59 0.01374826606103465 60-61 0.017084398629168253 62-63 0.016999401621062938 64-65 0.018231858238590002 66-67 0.00960466191590056 68-69 0.012558307947560247 70-71 0.01876308953924822 72-73 0.01583069275961486 74-75 0.01330203176848175 76-77 0.009243424631452973 78-79 0.014746980906272099 80-81 0.017998116466300385 82-83 0.015256962954903988 84-85 0.013238284012402764 86-87 0.0037611176086601752 88-89 0.028452748463254095 90-91 0.03242635859217755 92-93 0.017020650873089266 94-95 0.0332763286732307 96-97 0.027029048577490073 98-99 0.023756663765435455 100-101 0.15186840423217104 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2353024.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.03943691740132 #Duplication Level Percentage of deduplicated Percentage of total 1 73.83593589761294 37.68544592483503 2 14.282619290225574 14.579536925574546 3 5.185835168556104 7.940463208486815 4 2.3311265241284356 4.759175406989372 5 1.2471985400582246 3.182815560438839 6 0.7427244846792358 2.2744943689677157 7 0.49148240124568854 1.755948951007457 8 0.3317263450046835 1.354490068776532 9 0.2665951184492753 1.2246178257521028 >10 1.152920152403794 10.451163673665741 >50 0.07783913589782337 2.764906942551712 >100 0.04503090898386437 4.5641220125130175 >500 0.0041897349319923675 1.5043336739036899 >1k 0.004524913726551413 4.805237105004821 >5k 1.6758939727968194E-4 0.6526449042977488 >10k+ 8.379469863984097E-5 0.5006034472349377 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11705 0.4974449899363543 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9373 0.3983384784855573 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5887 0.25018869335799376 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 4642 0.1972780558124354 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 4436 0.18852336397758798 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 4281 0.1819360958494261 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 4177 0.17751625142794972 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3875 0.1646817032040472 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 3711 0.15771194853941142 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 3437 0.1460673584289833 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 3171 0.13476275635097643 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 2984 0.1268155360931295 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 2979 0.12660304357286623 No Hit GGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCT 2532 0.1076062122613284 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 2518 0.1070112332045912 No Hit GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA 2509 0.10662874666811728 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.499700810531469E-5 2 0.0 0.0 0.0 0.0 8.499700810531469E-5 3 0.0 0.0 0.0 0.0 8.499700810531469E-5 4 0.0 0.0 0.0 4.2498504052657345E-5 8.499700810531469E-5 5 0.0 0.0 0.0 8.499700810531469E-5 1.2749551215797205E-4 6 0.0 0.0 0.0 8.499700810531469E-5 2.1249252026328674E-4 7 0.0 0.0 0.0 1.2749551215797205E-4 2.1249252026328674E-4 8 0.0 0.0 0.0 1.6999401621062938E-4 2.1249252026328674E-4 9 0.0 0.0 0.0 2.9748952836860143E-4 2.1249252026328674E-4 10-11 0.0 0.0 0.0 3.824865364739161E-4 2.1249252026328674E-4 12-13 0.0 0.0 0.0 4.249850405265735E-4 4.887327966055595E-4 14-15 0.0 0.0 0.0 4.4623429255290215E-4 6.799760648425175E-4 16-17 0.0 0.0 0.0 5.099820486318882E-4 8.924685851058043E-4 18-19 0.0 0.0 0.0 5.099820486318882E-4 0.0010837118533427623 20-21 0.0 0.0 0.0 5.524805526845455E-4 0.0012324566175270632 22-23 0.0 0.0 0.0 6.587268128161888E-4 0.0013174536256323778 24-25 0.0 0.0 0.0 9.987148452374477E-4 0.001359952129685035 26-27 0.0 0.0 0.0 0.0017636879181852799 0.0013812013817113638 28-29 0.0 0.0 0.0 0.0038461146167654897 0.0014024506337376925 30-31 0.0 0.0 0.0 0.01119835581787521 0.00144494913779035 32-33 0.0 0.0 0.0 0.02641282026872654 0.0015299461458956644 34-35 0.0 0.0 0.0 0.04963825273350378 0.0015299461458956644 36-37 0.0 0.0 0.0 0.08890687047815918 0.001551195397921993 38-39 0.0 0.0 0.0 0.14596111216885166 0.0016149431540009793 40-41 0.0 0.0 0.0 0.20662772670402002 0.0016149431540009793 42-43 0.0 0.0 0.0 0.27124670211608554 0.001636192406027308 44-45 0.0 0.0 0.0 0.3522276015884241 0.0017424386661589512 46-47 0.0 0.0 0.0 0.44126196757874125 0.0018699341783169233 48-49 0.0 0.0 0.0 0.5327399975520861 0.0019124326823695807 50-51 0.0 0.0 0.0 0.6360538609040962 0.0019124326823695807 52-53 0.0 0.0 0.0 0.7298693086003373 0.002018678942501224 54-55 0.0 0.0 0.0 0.8297832916281347 0.002039928194527553 56-57 0.0 0.0 0.0 0.9488640999836806 0.0020611774465538815 58-59 0.0 0.0 0.0 1.071408536419518 0.0021036759506065385 60-61 0.0 0.0 0.0 1.2084874612413643 0.0021249252026328672 62-63 0.0 0.0 4.2498504052657345E-5 1.34099779687755 0.002209922210738182 64-65 0.0 0.0 4.2498504052657345E-5 1.4768867635859217 0.002337417722896154 66-67 0.0 0.0 4.2498504052657345E-5 1.6367023880759397 0.0023586669749224827 68-69 0.0 0.0 4.2498504052657345E-5 1.8082051011804383 0.0023799162269488115 70-71 0.0 0.0 4.2498504052657345E-5 1.9829377005929392 0.002422414731001469 72-73 0.0 0.0 4.2498504052657345E-5 2.1650225412065494 0.0026349072512647552 74-75 0.0 0.0 4.2498504052657345E-5 2.347702360876897 0.0027836520154490563 76-77 0.0 0.0 4.2498504052657345E-5 2.544172945112332 0.0028898982755806994 78-79 0.0 0.0 4.2498504052657345E-5 2.7656963974868085 0.0028898982755806994 80-81 0.0 0.0 4.2498504052657345E-5 2.99569830141979 0.0028898982755806994 82-83 0.0 0.0 4.2498504052657345E-5 3.236898561170647 0.0030598922917913288 84-85 0.0 0.0 4.2498504052657345E-5 3.4841123592449543 0.0030598922917913288 86-87 0.0 0.0 4.2498504052657345E-5 3.732792355709079 0.003102390795843986 88-89 0.0 0.0 4.2498504052657345E-5 3.989143332154708 0.0031448892998966436 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6085 0.0 47.357876 1 GTATCAA 10260 0.0 39.85193 1 TATCAAC 12815 0.0 31.7458 2 ATCAACG 12825 0.0 31.647604 3 TCAACGC 13065 0.0 31.066252 4 CAACGCA 13165 0.0 30.721972 5 AACGCAG 13485 0.0 30.024986 6 ACGCAGA 14965 0.0 26.920553 7 CGCAGAG 15090 0.0 26.57162 8 TAAGGTG 2085 0.0 25.758038 5 AGGTAAG 1835 0.0 25.640722 2 TAAGGTA 1010 0.0 24.939909 4 AAGGTAA 1770 0.0 23.990131 1 GGTAAGG 2100 0.0 23.989817 3 GCAGAGT 16465 0.0 23.946623 9 GTGTAAG 1185 0.0 23.7547 1 GAGTACT 11125 0.0 23.22905 12-13 GTAAGGT 2215 0.0 22.958864 4 GTACATG 7125 0.0 22.365442 1 TACATGG 6940 0.0 22.187925 2 >>END_MODULE