FastQCFastQC Report
Thu 26 May 2016
SRR937998_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937998_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2353024
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112080.4763232334221835No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94150.40012341565576887No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60160.2556710003807866No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC48340.2054377685905456No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA47270.20089042865691129No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA42280.17968367513463526No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA42250.1795561796224773No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA42210.17938618560626665No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG38170.16221678996899308No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA33310.14156251699940162No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA31350.13323281020508076No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA30220.1284304792471305No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT28810.12243819017570583No Hit
GGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCT26770.11376849534896372No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA25900.11007112549638254No Hit
GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA23870.10144392917369309No Hit
TCAGTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAG23800.10114643964532449No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61000.047.4278261
GTATCAA103200.039.1737941
ATCAACG126950.031.2804663
TATCAAC127550.031.2111192
TCAACGC129550.030.9060614
CAACGCA130950.030.648185
AACGCAG133950.029.9617736
AGGTAAG19700.028.214052
TAAGGTG21850.027.3887755
ACGCAGA150500.026.5722947
GGTAAGG21400.025.9699863
CGCAGAG153100.025.9659218
GTAAGGT22850.024.9429914
AAGGTAA20750.024.7258631
GTGTAAG11900.023.553251
GCAGAGT167900.023.3942059
GAGTACT113000.022.8861612-13
GTACTTT116600.022.11845614-15
TAAGGTA11500.021.4762764
AGAGTAC143400.021.14780210-11
Produced by FastQC (version 0.11.5)