##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937998_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2353024 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.493750594979055 33.0 31.0 34.0 31.0 34.0 2 32.73078302643747 34.0 31.0 34.0 31.0 34.0 3 32.688691233068596 34.0 31.0 34.0 31.0 34.0 4 36.217607640211064 37.0 35.0 37.0 35.0 37.0 5 36.18055446948267 37.0 35.0 37.0 35.0 37.0 6 36.24156404694555 37.0 37.0 37.0 35.0 37.0 7 36.20393077163684 37.0 37.0 37.0 35.0 37.0 8 36.19504518440951 37.0 37.0 37.0 35.0 37.0 9 37.904632081950716 39.0 38.0 39.0 35.0 39.0 10-11 37.86759973548931 39.0 38.0 39.0 35.0 39.0 12-13 37.81269358068596 39.0 38.0 39.0 35.0 39.0 14-15 39.08655840314421 40.0 38.5 41.0 36.0 41.0 16-17 39.02014174101072 40.0 38.0 41.0 36.0 41.0 18-19 39.028113185415876 40.0 38.5 41.0 36.0 41.0 20-21 39.03040215484415 40.0 38.5 41.0 35.5 41.0 22-23 39.058785205760756 40.0 39.0 41.0 35.5 41.0 24-25 38.99529647806397 40.0 38.5 41.0 35.0 41.0 26-27 38.81995763749116 40.0 38.0 41.0 35.0 41.0 28-29 38.735597256976554 40.0 38.0 41.0 35.0 41.0 30-31 38.492387242969045 40.0 38.0 41.0 34.0 41.0 32-33 38.26377355267094 40.0 38.0 41.0 34.0 41.0 34-35 38.040578209147036 40.0 38.0 41.0 33.5 41.0 36-37 37.92581206141544 40.0 37.5 41.0 33.0 41.0 38-39 37.847692161235926 40.0 37.0 41.0 33.0 41.0 40-41 37.70972799257466 40.0 37.0 41.0 33.0 41.0 42-43 37.5050031363896 40.0 37.0 41.0 33.0 41.0 44-45 37.24763453326443 40.0 37.0 41.0 32.0 41.0 46-47 36.873177664146226 40.0 36.0 41.0 31.0 41.0 48-49 36.4640058919926 39.5 36.0 41.0 30.0 41.0 50-51 36.13600095026655 39.0 35.0 41.0 29.5 41.0 52-53 35.649981470652236 39.0 35.0 40.0 28.0 41.0 54-55 35.3140509829054 38.0 35.0 40.0 27.5 41.0 56-57 35.0203093976092 38.0 34.0 40.0 27.0 41.0 58-59 34.61699795667192 38.0 34.0 40.0 26.0 41.0 60-61 34.18316260267639 37.0 34.0 40.0 25.5 41.0 62-63 33.7461084119839 37.0 33.0 39.5 24.0 41.0 64-65 33.29931335167002 36.0 33.0 39.0 22.5 40.5 66-67 32.77611129338247 35.5 32.0 39.0 21.5 40.0 68-69 32.2325692385628 35.0 31.5 38.0 19.5 40.0 70-71 31.619531292498504 35.0 31.0 37.5 18.0 39.5 72-73 31.146155117839854 35.0 30.5 37.0 15.5 39.0 74-75 30.595085727982372 34.0 30.0 36.0 11.0 39.0 76-77 29.39729280279334 33.0 28.5 35.0 10.0 37.0 78-79 30.085694833541858 34.0 30.0 35.0 7.5 37.5 80-81 29.896701011124406 34.0 30.0 35.0 7.0 37.0 82-83 29.434396759234076 34.0 29.0 35.0 2.0 36.5 84-85 28.912551253195886 34.0 29.0 35.0 2.0 36.0 86-87 28.37738182865963 33.5 28.5 35.0 2.0 36.0 88-89 27.724571232592613 33.0 26.5 35.0 2.0 35.0 90-91 27.183535102064404 33.0 26.0 35.0 2.0 35.0 92-93 26.542274111951258 32.5 25.0 34.5 2.0 35.0 94-95 25.770889289697003 32.0 23.5 34.0 2.0 35.0 96-97 24.926036241024313 32.0 19.0 34.0 2.0 35.0 98-99 23.857998260961214 31.0 2.0 34.0 2.0 35.0 100-101 20.32974121810912 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1011568190282972 1101 2 0.05602617216612771 1101 3 0.17429511712521162 1101 4 0.05685424459060329 1101 5 0.03248243992590005 1101 6 0.031094036647196788 1101 7 0.11870904878377786 1101 8 0.1479365017799239 1101 9 0.13373893139841186 1101 10-11 0.1395570051156696 1101 12-13 0.07458746575145625 1101 14-15 0.21598641390677642 1101 16-17 0.22981469007716981 1101 18-19 0.2766404041613484 1101 20-21 0.26643004672882853 1101 22-23 0.18609901006462337 1101 24-25 0.20745756620717515 1101 26-27 0.29969781847842825 1101 28-29 0.18544500176300005 1101 30-31 0.25811809974401 1101 32-33 0.27989252697894074 1101 34-35 0.14293261565975257 1101 36-37 0.23798823784535017 1101 38-39 0.3777547644473742 1101 40-41 0.3451664150151501 1101 42-43 0.3794518984419426 1101 44-45 0.3533365863542741 1101 46-47 0.34634011161168843 1101 48-49 0.4177389073710387 1101 50-51 0.5398521667940983 1101 52-53 0.39328256702982856 1101 54-55 0.4251368367027979 1101 56-57 0.5723921146321942 1101 58-59 0.5248753284557139 1101 60-61 0.5551445335559464 1101 62-63 0.6929895987730461 1101 64-65 0.669283211613326 1101 66-67 0.6001318785300853 1101 68-69 0.6780933747869398 1101 70-71 0.8726223173660337 1101 72-73 0.9732231418717276 1101 74-75 1.0764348543955933 1101 76-77 1.1972103866900312 1101 78-79 1.2322559139172675 1101 80-81 1.2719432108446185 1101 82-83 1.3555382905014568 1101 84-85 1.4398468235183337 1101 86-87 1.5177477866979103 1101 88-89 1.702492893133357 1101 90-91 1.860474787037333 1101 92-93 2.2828963086063006 1101 94-95 2.303183582483765 1101 96-97 2.375822150247746 1101 98-99 2.6548075902941584 1101 100-101 3.055068324125717 1102 1 0.06434438238316176 1102 2 0.08982092576550116 1102 3 0.24987592152935179 1102 4 0.13823691207407762 1102 5 0.11053013497646447 1102 6 0.12709261914655912 1102 7 0.1614799328811216 1102 8 0.0792055769156974 1102 9 0.121651716649815 1102 10-11 0.18261564733686697 1102 12-13 0.15120735855983725 1102 14-15 0.19000714348007364 1102 16-17 0.25110947801522343 1102 18-19 0.1891962731908805 1102 20-21 0.28096080182262995 1102 22-23 0.3193069843298417 1102 24-25 0.18487576460781696 1102 26-27 0.24865818065533318 1102 28-29 0.04942675006873287 1102 30-31 -0.04104115205739589 1102 32-33 0.09129626467539254 1102 34-35 -0.017299898398498215 1102 36-37 0.21380625383893204 1102 38-39 0.2998588463547378 1102 40-41 0.27484492257378434 1102 42-43 0.2689809366018281 1102 44-45 0.25260544721670186 1102 46-47 0.2286648950719794 1102 48-49 0.2073520066153165 1102 50-51 0.3040333541431792 1102 52-53 0.23898858399039113 1102 54-55 0.022992454922622585 1102 56-57 0.2554708381124371 1102 58-59 0.3179106203756774 1102 60-61 0.28711123327931176 1102 62-63 0.28404821901748534 1102 64-65 0.3593484825729689 1102 66-67 0.3650389855376517 1102 68-69 0.3752440886193895 1102 70-71 0.5031649234244746 1102 72-73 0.6372342840860483 1102 74-75 0.6036372685420304 1102 76-77 0.6452367368181839 1102 78-79 0.8439060659689339 1102 80-81 0.9966253033612595 1102 82-83 1.0250065354653444 1102 84-85 1.0293073216764093 1102 86-87 1.0845152259390538 1102 88-89 1.240185086715595 1102 90-91 1.14812371388496 1102 92-93 1.4433987246757027 1102 94-95 1.6685690959568866 1102 96-97 1.923398388981223 1102 98-99 2.2875361966108265 1102 100-101 2.4801166043073586 1103 1 0.27719061227216457 1103 2 0.3214685333697531 1103 3 0.3459398607474995 1103 4 0.17769299270224082 1103 5 0.12039866565994117 1103 6 0.16142048179601431 1103 7 0.1965131917490055 1103 8 0.15456569859967573 1103 9 0.25838281284556075 1103 10-11 0.3305417448669701 1103 12-13 0.273440119265139 1103 14-15 0.4119746601721559 1103 16-17 0.3963355243007385 1103 18-19 0.4576413819634837 1103 20-21 0.4227534604396723 1103 22-23 0.42656041201670547 1103 24-25 0.45493589243112353 1103 26-27 0.4577232111702969 1103 28-29 0.5478669849669231 1103 30-31 0.5766243247491047 1103 32-33 0.664918580755554 1103 34-35 0.659825718509957 1103 36-37 0.6219059064031072 1103 38-39 0.6465112213348476 1103 40-41 0.8135582698479809 1103 42-43 0.7646319120658163 1103 44-45 0.8522656542248015 1103 46-47 0.9068121462193446 1103 48-49 0.8888727556634066 1103 50-51 0.9536617995275947 1103 52-53 0.9363746086253784 1103 54-55 1.0566617807116856 1103 56-57 1.10188252638995 1103 58-59 1.1836113243936879 1103 60-61 1.1822906081454079 1103 62-63 1.2891408539542653 1103 64-65 1.0264523361913476 1103 66-67 1.0889639454127575 1103 68-69 1.1520677508410913 1103 70-71 1.3137238152382444 1103 72-73 1.318186706802564 1103 74-75 1.285801268777579 1103 76-77 0.894545435994889 1103 78-79 1.3417948890097087 1103 80-81 1.432239668385634 1103 82-83 1.4490222070388796 1103 84-85 1.726401164431941 1103 86-87 1.6967022925056519 1103 88-89 1.885246248201213 1103 90-91 1.9705520560284349 1103 92-93 2.144359216222849 1103 94-95 2.074788610749536 1103 96-97 2.237767172128894 1103 98-99 2.8432255366080454 1103 100-101 3.237933158691149 1104 1 0.09081681346085446 1104 2 0.07443933453252072 1104 3 0.1745466670205289 1104 4 0.05715798927688809 1104 5 0.03227076105144988 1104 6 0.08788921848805131 1104 7 0.09681152165698137 1104 8 0.04273572581735863 1104 9 0.04136828748867316 1104 10-11 0.10830609895611332 1104 12-13 0.06823184379252467 1104 14-15 0.1397789381628627 1104 16-17 0.18719202506913746 1104 18-19 0.18379674495853493 1104 20-21 0.15369564137247949 1104 22-23 0.17553917018663867 1104 24-25 0.19248056091230836 1104 26-27 0.273366090134914 1104 28-29 0.15922944307575904 1104 30-31 0.21108733091473653 1104 32-33 0.27375148360507495 1104 34-35 0.26195522470336385 1104 36-37 0.2565433047996706 1104 38-39 0.33936785171126616 1104 40-41 0.38345437512673186 1104 42-43 0.4912154444400514 1104 44-45 0.5106602042072126 1104 46-47 0.43505095826919415 1104 48-49 0.41471324058258574 1104 50-51 0.4718871184559248 1104 52-53 0.45355993884811596 1104 54-55 0.5832426514924833 1104 56-57 0.7239474831564152 1104 58-59 0.8052057577288636 1104 60-61 0.8512160418694137 1104 62-63 0.708973891040678 1104 64-65 0.7144565345656986 1104 66-67 0.6403550330092713 1104 68-69 0.9540332797344249 1104 70-71 0.9121837941306516 1104 72-73 0.8606451396563486 1104 74-75 0.9191288160382172 1104 76-77 0.8621607871400663 1104 78-79 1.0863964831884552 1104 80-81 1.1665338369501335 1104 82-83 1.2118369693933033 1104 84-85 1.2689735829177486 1104 86-87 1.379732435629947 1104 88-89 1.6303977507285694 1104 90-91 1.704026338092536 1104 92-93 1.8383244581269764 1104 94-95 1.7735389347696042 1104 96-97 2.158300902618258 1104 98-99 2.452909802193414 1104 100-101 2.831890647257868 1105 1 0.06252981372945499 1105 2 0.04914611222076104 1105 3 0.16338855080600467 1105 4 0.0810522501775921 1105 5 0.056265747173753766 1105 6 0.07363100378664456 1105 7 0.028961893221136847 1105 8 0.005635269196353931 1105 9 0.035140111286686704 1105 10-11 0.012762630427140209 1105 12-13 0.008722823289353698 1105 14-15 0.0018721201845295354 1105 16-17 0.11062127119680554 1105 18-19 0.14286872991690558 1105 20-21 0.14137079134114572 1105 22-23 0.10061359493371214 1105 24-25 0.08669669485446008 1105 26-27 0.00907150243681798 1105 28-29 0.013032379775552272 1105 30-31 0.09177830593488068 1105 32-33 -0.09220842775673077 1105 34-35 0.024529089865872322 1105 36-37 0.06281233067485203 1105 38-39 0.0879269214578926 1105 40-41 0.04432654362883426 1105 42-43 0.17925824146754366 1105 44-45 0.26750672135288767 1105 46-47 0.1537813728094335 1105 48-49 0.06381174984930027 1105 50-51 0.17234989489070074 1105 52-53 0.14058988781400217 1105 54-55 0.21202264522514014 1105 56-57 0.2606646243507953 1105 58-59 0.18226782684875076 1105 60-61 0.2382163328533906 1105 62-63 0.26671742224311146 1105 64-65 0.5080173949829216 1105 66-67 0.5647187853595241 1105 68-69 0.465837716120852 1105 70-71 0.5984918790084457 1105 72-73 0.4652258989006839 1105 74-75 0.3911328002803174 1105 76-77 0.5706228249232161 1105 78-79 0.8009244176499131 1105 80-81 0.9210692698447502 1105 82-83 0.9072383034429023 1105 84-85 1.0487798547415466 1105 86-87 1.1298779276166933 1105 88-89 1.3169240735864811 1105 90-91 1.3080587044637433 1105 92-93 1.3572188309445963 1105 94-95 1.390276668675952 1105 96-97 1.6446106662497364 1105 98-99 1.9510351939026087 1105 100-101 2.438502698471048 1106 1 0.04744157340110888 1106 2 0.0667969385979248 1106 3 0.06055187523037375 1106 4 0.07835536383331743 1106 5 0.08780260700224574 1106 6 0.012494651148820424 1106 7 0.02583336215457166 1106 8 0.031512191663672695 1106 9 -0.045493331403136494 1106 10-11 0.0459725601395391 1106 12-13 0.016513539582490466 1106 14-15 0.007759166328291656 1106 16-17 0.030873366005351954 1106 18-19 -0.04669914142503018 1106 20-21 -0.09555725910250601 1106 22-23 -0.03454398137305503 1106 24-25 -0.024507766058952996 1106 26-27 -0.027672538738634955 1106 28-29 -0.05880902180636838 1106 30-31 0.0021390297079193488 1106 32-33 0.12330440937030573 1106 34-35 -0.20277229266391572 1106 36-37 -0.08269643684467809 1106 38-39 0.08346984055194895 1106 40-41 0.16071918572650645 1106 42-43 0.08038529214535828 1106 44-45 0.20967590984457019 1106 46-47 -0.04814090775573732 1106 48-49 0.07289260966999933 1106 50-51 0.013664676919859176 1106 52-53 0.09979929916298147 1106 54-55 0.08124338846602797 1106 56-57 0.2774998240847282 1106 58-59 0.3373636380111762 1106 60-61 0.30548787051219506 1106 62-63 0.25120711847110755 1106 64-65 0.35235628838096744 1106 66-67 0.359490314086635 1106 68-69 0.15071457720303272 1106 70-71 0.3361796923690292 1106 72-73 0.3517989873300351 1106 74-75 0.4604423083839677 1106 76-77 0.5637492318690285 1106 78-79 0.722070250881746 1106 80-81 0.9076839799980831 1106 82-83 0.8408592050509682 1106 84-85 0.943148044116981 1106 86-87 1.1375259802948925 1106 88-89 1.331841962028566 1106 90-91 1.237911808846743 1106 92-93 1.136289729483405 1106 94-95 1.60476236025427 1106 96-97 1.8112952775145281 1106 98-99 2.17343826952553 1106 100-101 2.600830669861029 1107 1 0.09007026143405028 1107 2 0.07994489733893317 1107 3 0.17895410665396838 1107 4 0.0857680254802844 1107 5 0.05893737683491196 1107 6 0.05848826646327865 1107 7 0.005717068435188821 1107 8 0.046329670207107654 1107 9 0.06763785526128885 1107 10-11 0.031149668718249757 1107 12-13 0.015689448541969853 1107 14-15 0.14036910383858014 1107 16-17 0.07280347900584161 1107 18-19 -0.010826744764322882 1107 20-21 0.054258648137405885 1107 22-23 0.016089411627447703 1107 24-25 0.03206425996867068 1107 26-27 0.049589624507795804 1107 28-29 0.07035079443191705 1107 30-31 0.14758556781168153 1107 32-33 0.09296114976036307 1107 34-35 0.03808359158145436 1107 36-37 0.14110386153825516 1107 38-39 0.08619377326972 1107 40-41 0.1663574432567998 1107 42-43 0.20959280446671613 1107 44-45 0.2266765040059724 1107 46-47 0.28138685866022684 1107 48-49 0.2804032836297736 1107 50-51 0.2609945984142996 1107 52-53 0.18186127300575805 1107 54-55 0.20630455790809066 1107 56-57 0.2866173853588947 1107 58-59 0.37029213514995263 1107 60-61 0.41421493579574076 1107 62-63 0.4155506407232892 1107 64-65 0.35600605855331224 1107 66-67 0.5224649273757365 1107 68-69 0.459564522658475 1107 70-71 0.6643924432578778 1107 72-73 0.35831993618712943 1107 74-75 0.4445584929044273 1107 76-77 0.35950738699708396 1107 78-79 0.5710432710589153 1107 80-81 0.7049052595427838 1107 82-83 0.9411928579794342 1107 84-85 1.1224697838504376 1107 86-87 1.2885742193915881 1107 88-89 1.370259431095537 1107 90-91 1.445659708061779 1107 92-93 1.6722767379489873 1107 94-95 1.580976135353744 1107 96-97 1.7137530221581692 1107 98-99 2.0111610545563003 1107 100-101 2.222268505939944 1108 1 0.042368465249978726 1108 2 0.06483886658918436 1108 3 0.13153084850116414 1108 4 0.036229604952985994 1108 5 -0.0073060554079447115 1108 6 0.015178707565134175 1108 7 0.010691827346057892 1108 8 -0.014464625140142573 1108 9 0.04079702785641359 1108 10-11 0.040522344728081805 1108 12-13 0.048049085215687626 1108 14-15 0.12665538459097547 1108 16-17 0.10311179440358842 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Count 2 3.0 3 7.0 4 18.0 5 37.0 6 85.0 7 212.0 8 595.0 9 1165.0 10 1993.0 11 3208.0 12 4931.0 13 7167.0 14 10093.0 15 13386.0 16 17377.0 17 24448.0 18 24550.0 19 19122.0 20 16503.0 21 16142.0 22 16760.0 23 18111.0 24 20660.0 25 23746.0 26 27453.0 27 32894.0 28 40126.0 29 48253.0 30 59120.0 31 73116.0 32 91669.0 33 121058.0 34 167404.0 35 250405.0 36 380137.0 37 503643.0 38 299638.0 39 17789.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.639981572648644 18.909496885709622 15.403540295381601 23.04698124626013 2 15.517584334420276 20.133663383169498 39.71776318697572 24.6309890954345 3 19.201312770784476 23.73179151051092 30.44414391517481 26.62275180352979 4 13.046403266605017 17.267864671163576 36.858867567861616 32.8268644943698 5 13.526116112504361 37.250272098175486 34.4762944159632 14.747317373356946 6 32.065005822304954 36.722552294498136 16.806529481262377 14.405912401934534 7 28.133882187347005 33.423373497252896 20.359885832018712 18.082858483381383 8 26.78242140635379 34.33819757664721 19.627704130137385 19.251676886861613 9 26.333900546700757 16.61627760702823 20.126909032802047 36.92291281346897 10-11 24.873205713158896 26.446032849643693 27.113896840831202 21.56686459636621 12-13 26.242741255507806 23.80693949572975 27.43112692433226 22.51919232443018 14-15 22.499791757330144 25.258624646412446 26.059147718000325 26.18243587825709 16-17 21.882611882116773 28.333779921874985 27.316153026096003 22.467455169912242 18-19 21.989788459446228 26.46513592721536 29.94429721073818 21.60077840260023 20-21 22.081181492411467 25.61393338954469 30.545587295327202 21.759297822716643 22-23 21.962971903389 25.549378161888704 30.552004569439156 21.93564536528314 24-25 22.056227326662654 25.817366373931993 30.37736338198645 21.749042917418905 26-27 21.396778773400438 26.2451966401986 30.100743033002907 22.25728155339806 28-29 21.682487139380452 26.86870236566552 29.814006829106383 21.634803665847645 30-31 21.699985550484058 26.16641592506651 29.707206058597034 22.426392465852395 32-33 21.286504150455528 26.148305445816707 29.836805911936775 22.728384491790994 34-35 22.459196936890528 26.340811734212004 29.382583495491453 21.817407833406016 36-37 22.614881688951392 25.669524696595968 29.469244567586173 22.246349046866463 38-39 21.79847920947968 26.228673725885766 29.308405240068495 22.664441824566055 40-41 22.45568334776141 25.962885448018863 29.485475606845306 22.095955597374424 42-43 22.131118622511874 26.031381823808154 29.22813839491416 22.60936115876581 44-45 21.99964637592704 26.309291004985162 29.555747007477535 22.13531561161026 46-47 22.778661413725434 26.77907790906589 28.42618948200517 22.016071195203505 48-49 22.256577613695427 26.78187043963521 28.2894443266289 22.672107620040467 50-51 22.452257180547246 27.029579808790732 28.376166158951204 22.141996851710818 52-53 23.04912742071479 26.504808280748517 28.345333494261006 22.10073080427569 54-55 22.183879127454713 26.88795354403525 28.538744186204646 22.38942314230539 56-57 22.448049828646035 27.017106497851273 28.187940284501984 22.34690338900071 58-59 22.409652430234456 26.54958045476799 28.16086873742044 22.87989837757711 60-61 21.744380847794158 27.005801895773267 28.613775295109612 22.63604196132296 62-63 22.012482660610345 27.037080794756026 28.726800066637654 22.223636477995974 64-65 21.622962621715715 27.1006585568188 28.472340273622372 22.804038547843117 66-67 21.517282822828836 27.106876261546088 28.443289047607205 22.93255186801787 68-69 22.374742723915723 26.61744475284284 28.575399157762142 22.4324133654793 70-71 22.09911586112169 27.025861189686122 27.840387518359357 23.034635430832832 72-73 22.0113139517489 27.053825205352773 28.079654096175815 22.855206746722516 74-75 22.114245328564436 27.552013919110045 27.805453748028068 22.528287004297447 76-77 22.20007105749878 27.0034857273024 28.104239480770275 22.69220373442855 78-79 22.247095652233043 27.419822322254255 27.902265340260023 22.43081668525268 80-81 22.572682158537607 27.411423367172134 27.546356404261928 22.46953807002833 82-83 22.45466534013373 27.555810643514388 27.72240640781708 22.267117608534804 84-85 22.294456375251336 27.77851681252096 27.630785420152588 22.29624139207512 86-87 22.69029488237599 27.77187988470918 27.114104256427733 22.423720976487097 88-89 22.3139860145804 27.94653003669324 26.942544523595615 22.796939425130745 90-91 22.11306529192826 27.892151602309035 27.215251606240297 22.779531499522403 92-93 22.496030082339267 27.367389450569345 27.49197463587573 22.644605831215657 94-95 22.118594276085332 27.14145317352469 27.713865889510192 23.026086660879784 96-97 22.370780844005445 26.710824141591775 27.81980418339341 23.098590831009368 98-99 22.024844667703633 27.180878190580614 27.220656857995255 23.573620283720494 100-101 22.20407245617051 26.641389215723983 27.74493843588494 23.409599892220566 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 694.0 1 498.0 2 442.0 3 563.0 4 447.0 5 442.5 6 879.5 7 2153.5 8 3622.0 9 4166.0 10 3418.5 11 2426.0 12 2288.5 13 2446.5 14 2599.0 15 2783.0 16 3009.0 17 3226.0 18 3596.5 19 4032.0 20 4439.5 21 5249.5 22 6481.0 23 7929.5 24 9951.5 25 12859.0 26 16339.5 27 20294.0 28 23536.5 29 26785.5 30 30785.5 31 36502.0 32 45462.0 33 48067.5 34 45163.0 35 46119.5 36 49878.5 37 53671.0 38 56561.0 39 59333.0 40 63416.0 41 68514.0 42 71433.5 43 73172.0 44 75691.0 45 81230.0 46 83771.0 47 85875.5 48 88064.5 49 85044.0 50 82294.5 51 79887.5 52 78484.5 53 80200.0 54 83752.5 55 79571.0 56 72113.0 57 66791.0 58 62016.5 59 54547.5 60 42123.5 61 34870.5 62 31332.5 63 22721.5 64 14248.5 65 10556.0 66 8070.0 67 6328.0 68 4841.0 69 3849.5 70 3072.5 71 2002.0 72 1305.0 73 981.0 74 701.5 75 505.5 76 354.0 77 219.0 78 123.0 79 66.0 80 30.0 81 15.5 82 17.0 83 11.5 84 6.0 85 4.0 86 2.0 87 1.5 88 1.5 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.010539629005059021 3 0.005864793559266714 4 0.0 5 5.524805526845455E-4 6 1.6999401621062938E-4 7 0.0 8 1.2749551215797205E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.1249252026328673E-5 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.001699940162106294 26-27 0.0214192460425393 28-29 3.187387803949301E-4 30-31 1.6999401621062938E-4 32-33 0.0038036161127128322 34-35 0.016234428548115108 36-37 0.007628481477451994 38-39 0.02114300576619703 40-41 0.02339542648098787 42-43 0.01920932383180112 44-45 0.010327136484795735 46-47 0.00240116547897514 48-49 2.1249252026328674E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 3.612372844475875E-4 68-69 2.9748952836860143E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.1249252026328674E-4 82-83 9.77465593211119E-4 84-85 0.004462342925529021 86-87 7.649730729478323E-4 88-89 8.499700810531469E-5 90-91 0.003888613120818147 92-93 6.587268128161888E-4 94-95 6.374775607898603E-5 96-97 2.549910243159441E-4 98-99 1.6999401621062938E-4 100-101 0.002996144535712343 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2353024.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.1654915422638 #Duplication Level Percentage of deduplicated Percentage of total 1 72.15538840709984 35.475551384580264 2 15.04362308641007 14.792542472397974 3 5.499920189780164 8.112188387212878 4 2.544145461741348 5.0033664872613235 5 1.3938034927164948 3.426351691636529 6 0.8288996982136981 2.4451956661146355 7 0.5263287595577814 1.8114048523541808 8 0.35692073464780705 1.4038546688468272 9 0.26744614627026025 1.1834209118215375 >10 1.241840589476823 10.951328348066973 >50 0.08161526819492274 2.7803401113442208 >100 0.05017853987195544 4.865084469397848 >500 0.004684559693167374 1.5749959065531167 >1k 0.004944813009454076 5.03817847281582 >5k 1.7350221085803776E-4 0.6581569538283576 >10k+ 8.675110542901888E-5 0.47803921576749603 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11208 0.4763232334221835 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9415 0.40012341565576887 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6016 0.2556710003807866 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 4834 0.2054377685905456 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 4727 0.20089042865691129 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 4228 0.17968367513463526 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 4225 0.1795561796224773 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 4221 0.17938618560626665 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 3817 0.16221678996899308 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 3331 0.14156251699940162 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 3135 0.13323281020508076 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 3022 0.1284304792471305 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 2881 0.12243819017570583 No Hit GGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCT 2677 0.11376849534896372 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 2590 0.11007112549638254 No Hit GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA 2387 0.10144392917369309 No Hit TCAGTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAG 2380 0.10114643964532449 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.2498504052657345E-5 4.2498504052657345E-5 5 0.0 0.0 0.0 8.499700810531469E-5 4.2498504052657345E-5 6 0.0 0.0 0.0 8.499700810531469E-5 4.2498504052657345E-5 7 0.0 0.0 0.0 1.6999401621062938E-4 4.2498504052657345E-5 8 0.0 0.0 0.0 2.1249252026328674E-4 4.2498504052657345E-5 9 0.0 0.0 0.0 2.1249252026328674E-4 4.2498504052657345E-5 10-11 0.0 0.0 0.0 2.9748952836860143E-4 8.499700810531469E-5 12-13 0.0 0.0 0.0 2.9748952836860143E-4 4.037357885002448E-4 14-15 0.0 0.0 0.0 2.9748952836860143E-4 5.099820486318882E-4 16-17 0.0 0.0 0.0 3.6123728444758743E-4 5.524805526845455E-4 18-19 0.0 0.0 0.0 3.824865364739161E-4 6.374775607898602E-4 20-21 0.0 0.0 0.0 4.674835445792308E-4 7.224745688951749E-4 22-23 0.0 0.0 0.0 5.737298047108741E-4 8.074715770004896E-4 24-25 0.0 0.0 0.0 8.924685851058043E-4 8.074715770004896E-4 26-27 0.0 0.0 0.0 0.0016361924060273078 9.562163411847903E-4 28-29 0.0 0.0 0.0 0.003761117608660175 0.0010199640972637764 30-31 0.0 0.0 0.0 0.011028361801664582 0.0010624626013164338 32-33 0.0 0.0 0.0 0.026349072512647558 0.001232456617527063 34-35 0.0 0.0 0.0 0.04965950198553011 0.001232456617527063 36-37 0.0 0.0 0.0 0.08924685851058042 0.001232456617527063 38-39 0.0 0.0 0.0 0.14661983898166786 0.001232456617527063 40-41 0.0 0.0 0.0 0.20760519229723112 0.001232456617527063 42-43 0.0 0.0 0.0 0.27239416172550723 0.0012537058695533917 44-45 0.0 0.0 0.0 0.3535025567100038 0.0013387028776587063 46-47 0.0 0.0 0.0 0.4428769107327422 0.0015086968938693359 48-49 0.0 0.0 0.0 0.5349286705107981 0.0018274356742642658 50-51 0.0 0.0 0.0 0.6384762756350977 0.0019974296904748954 52-53 0.0 0.0 0.0 0.7323342218353914 0.0021249252026328672 54-55 0.0 0.0 0.0 0.8325031958875048 0.0022949192188434966 56-57 0.0 0.0 0.0 0.951541505738998 0.002337417722896154 58-59 0.0 0.0 0.0 1.074574674971441 0.0023799162269488115 60-61 0.0 0.0 0.0 1.2115473535331556 0.0023799162269488115 62-63 0.0 0.0 0.0 1.3439514429092096 0.002464913235054126 64-65 0.0 0.0 0.0 1.4800529021378446 0.002464913235054126 66-67 0.0 0.0 0.0 1.640336010172442 0.0025711594951857695 68-69 0.0 0.0 0.0 1.8138999007234946 0.0026349072512647557 70-71 0.0 0.0 0.0 1.9902049447859436 0.0026774057553174127 72-73 0.0 4.2498504052657345E-5 0.0 2.1742234673339498 0.0027624027634227276 74-75 0.0 4.2498504052657345E-5 0.0 2.359771936027852 0.002911147527607028 76-77 0.0 4.2498504052657345E-5 0.0 2.559302412555078 0.0030173937877386718 78-79 0.0 4.2498504052657345E-5 0.0 2.7850119675787415 0.00320863705597563 80-81 0.0 4.2498504052657345E-5 0.0 3.019433715933199 0.0033361325681336017 82-83 0.0 4.2498504052657345E-5 0.0 3.265266312625796 0.0035273758363705597 84-85 0.0 4.2498504052657345E-5 0.0 3.518472399771528 0.0036123728444758746 86-87 0.0 4.2498504052657345E-5 0.0 3.7765233163792633 0.003761117608660175 88-89 0.0 4.2498504052657345E-5 0.0 4.043243927813741 0.0038673638687918184 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6100 0.0 47.427826 1 GTATCAA 10320 0.0 39.173794 1 ATCAACG 12695 0.0 31.280466 3 TATCAAC 12755 0.0 31.211119 2 TCAACGC 12955 0.0 30.906061 4 CAACGCA 13095 0.0 30.64818 5 AACGCAG 13395 0.0 29.961773 6 AGGTAAG 1970 0.0 28.21405 2 TAAGGTG 2185 0.0 27.388775 5 ACGCAGA 15050 0.0 26.572294 7 GGTAAGG 2140 0.0 25.969986 3 CGCAGAG 15310 0.0 25.965921 8 GTAAGGT 2285 0.0 24.942991 4 AAGGTAA 2075 0.0 24.725863 1 GTGTAAG 1190 0.0 23.55325 1 GCAGAGT 16790 0.0 23.394205 9 GAGTACT 11300 0.0 22.88616 12-13 GTACTTT 11660 0.0 22.118456 14-15 TAAGGTA 1150 0.0 21.476276 4 AGAGTAC 14340 0.0 21.147802 10-11 >>END_MODULE