FastQCFastQC Report
Thu 26 May 2016
SRR937997_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937997_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2405011
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120850.5024925041922885No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94800.3941769912902685No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63190.2627430810087771No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA47750.1985437904442017No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC46490.19330472916755892No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA45900.1908515179348452No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA43900.18253554765445978No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA40180.16706784293294294No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG36180.1504359023721721No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA35800.14885586801889888No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA32510.13517609690766488No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA31630.1315170699842953No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT30180.12548799153101586No Hit
GGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCT27070.11255665774501655No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA25570.1063196800347275No Hit
GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA25150.10457332627584655No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62750.047.23381
GTATCAA103300.038.256481
TATCAAC130800.029.7917822
TAAGGTG19800.029.5209475
ATCAACG131800.029.4936313
TCAACGC133550.029.2139054
CAACGCA134500.029.0075635
AACGCAG137850.028.2681566
AGGTAAG19600.025.9428752
GTAAGGT22200.025.2591884
ACGCAGA153400.025.1342987
CGCAGAG154350.024.8871968
GGTAAGG21600.024.640793
AAGGTAA20300.023.9816531
TAAGGTA11100.023.1185784
TAAGCGA1652.4221627E-623.0407414
GTGTAAG11450.022.9262071
GAGTACT111800.022.7685112-13
GCAGAGT170050.022.226029
GTACATG72950.022.1139551
Produced by FastQC (version 0.11.5)