##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937997_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2405011 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.904747629012924 33.0 31.0 34.0 30.0 34.0 2 32.09077338939406 34.0 31.0 34.0 30.0 34.0 3 32.15880551066087 34.0 31.0 34.0 30.0 34.0 4 35.68685922850249 37.0 35.0 37.0 35.0 37.0 5 35.708307363251144 37.0 35.0 37.0 35.0 37.0 6 35.739563769147004 37.0 36.0 37.0 35.0 37.0 7 35.68580850565756 37.0 35.0 37.0 33.0 37.0 8 35.66116412773164 37.0 35.0 37.0 33.0 37.0 9 37.331226343663296 39.0 37.0 39.0 34.0 39.0 10-11 37.2547017456469 39.0 37.0 39.0 34.0 39.0 12-13 37.16099656093049 39.0 37.0 39.0 34.0 39.0 14-15 38.410420576038945 40.0 38.0 41.0 34.0 41.0 16-17 38.329061904498566 40.0 38.0 41.0 34.0 41.0 18-19 38.283623027088026 40.0 38.0 41.0 33.5 41.0 20-21 38.17971082044947 40.0 38.0 41.0 33.5 41.0 22-23 38.03161835850231 40.0 38.0 41.0 33.0 41.0 24-25 37.969234028451424 40.0 38.0 41.0 33.0 41.0 26-27 37.968532992156796 40.0 38.0 41.0 33.0 41.0 28-29 37.87458789169779 40.0 38.0 41.0 33.0 41.0 30-31 37.655285984138956 40.0 37.5 41.0 32.5 41.0 32-33 37.40315616020051 40.0 37.0 41.0 32.0 41.0 34-35 37.17204661433981 40.0 36.5 41.0 31.0 41.0 36-37 36.92070992606686 40.0 36.0 41.0 30.5 41.0 38-39 36.65166001319744 39.5 36.0 41.0 30.0 41.0 40-41 36.40918149646717 39.0 35.5 41.0 30.0 41.0 42-43 36.108626530190506 39.0 35.0 41.0 29.5 41.0 44-45 35.85998899797132 39.0 35.0 40.5 28.0 41.0 46-47 35.669698808030404 39.0 35.0 41.0 27.0 41.0 48-49 35.373155673716255 39.0 35.0 41.0 26.0 41.0 50-51 34.70703023811534 38.5 34.5 40.0 24.5 40.5 52-53 34.76294100110145 38.5 34.5 40.0 24.5 41.0 54-55 34.87104029877618 39.0 35.0 40.0 24.0 41.0 56-57 34.637306440594244 38.0 34.5 40.0 23.5 41.0 58-59 34.36085094829088 38.0 34.0 40.0 23.0 41.0 60-61 33.972278089372566 37.5 34.0 40.0 22.0 41.0 62-63 33.54083931424846 37.0 33.5 40.0 20.5 41.0 64-65 33.106995144720756 36.5 33.0 39.5 18.5 41.0 66-67 32.67258153912809 36.0 32.5 39.0 17.5 41.0 68-69 32.18463054846735 35.0 32.0 39.0 12.5 40.0 70-71 31.68619311928303 35.0 31.0 38.0 8.0 40.0 72-73 31.2000787106587 35.0 31.0 37.0 7.0 39.5 74-75 30.680374642777103 35.0 30.5 37.0 2.0 39.0 76-77 30.14391535007532 34.5 30.0 36.0 2.0 39.0 78-79 29.569647082695255 34.0 29.0 36.0 2.0 38.0 80-81 29.04706132321224 34.0 29.0 35.0 2.0 37.0 82-83 28.497226000213722 34.0 28.5 35.0 2.0 37.0 84-85 28.005958600605155 34.0 27.0 35.0 2.0 36.0 86-87 27.515835478507167 33.5 26.0 35.0 2.0 36.0 88-89 26.949280481461415 33.0 25.5 35.0 2.0 35.0 90-91 26.33042426832975 32.5 24.5 35.0 2.0 35.0 92-93 25.715706705707376 32.0 22.0 35.0 2.0 35.0 94-95 25.096997477350413 32.0 19.0 34.0 2.0 35.0 96-97 24.247382236505363 31.5 8.5 34.0 2.0 35.0 98-99 23.146164196338397 31.0 2.0 34.0 2.0 35.0 100-101 20.228049476696782 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.46096424476455056 1101 2 0.430566391504108 1101 3 0.42554142975239273 1101 4 0.28200126074559506 1101 5 0.3677095510442996 1101 6 0.3125185508363302 1101 7 0.35238532430923186 1101 8 0.35735655957313384 1101 9 0.32198231940973443 1101 10-11 0.3653982254044337 1101 12-13 0.2819203271530668 1101 14-15 0.40854486381407895 1101 16-17 0.4356949796794751 1101 18-19 0.4921143958125924 1101 20-21 0.5685841244372085 1101 22-23 0.5774172023682524 1101 24-25 0.5592358203427139 1101 26-27 0.6343343820296283 1101 28-29 0.5696289193642272 1101 30-31 0.5862194351507952 1101 32-33 0.5024317025499343 1101 34-35 0.5362995106461383 1101 36-37 0.4981491891648133 1101 38-39 0.5782457914222832 1101 40-41 0.6373286476613345 1101 42-43 0.7163731360324093 1101 44-45 0.7620105218407431 1101 46-47 0.6712235144672789 1101 48-49 0.6413259658588615 1101 50-51 0.6944064061681274 1101 52-53 0.6507594916473423 1101 54-55 0.7000520417228486 1101 56-57 0.6332869643634709 1101 58-59 0.5254999901993287 1101 60-61 0.4719597219289966 1101 62-63 0.5732408057323042 1101 64-65 0.656835118213472 1101 66-67 0.7035676913820197 1101 68-69 0.6529288336419867 1101 70-71 0.7994525547961366 1101 72-73 0.7532194735136457 1101 74-75 0.8954604012461189 1101 76-77 0.8579946261624123 1101 78-79 0.8695060244466291 1101 80-81 0.9576367383046396 1101 82-83 0.8918352352259937 1101 84-85 1.0034421124230803 1101 86-87 1.141217476618273 1101 88-89 1.2133943988798315 1101 90-91 1.197279598869482 1101 92-93 1.2781630401418589 1101 94-95 1.4703295415339213 1101 96-97 1.4709491423587018 1101 98-99 1.4213689132615634 1101 100-101 1.2727322351328283 1102 1 0.40274257854440165 1102 2 0.31544217650385775 1102 3 0.2675564932713428 1102 4 0.23481008029062878 1102 5 0.2509665223869888 1102 6 0.28265677436430536 1102 7 0.18388226150471354 1102 8 0.20625782480580312 1102 9 0.24812498691797913 1102 10-11 0.32357849419258855 1102 12-13 0.3316095436896376 1102 14-15 0.4375338507349156 1102 16-17 0.30652742376266673 1102 18-19 0.37538498971059653 1102 20-21 0.37025447676190026 1102 22-23 0.41505431717158103 1102 24-25 0.35945026206768915 1102 26-27 0.3805597989933389 1102 28-29 0.44256018297732425 1102 30-31 0.4447285751286074 1102 32-33 0.47044253702227223 1102 34-35 0.49964405231525433 1102 36-37 0.625717230044593 1102 38-39 0.4695512333304137 1102 40-41 0.6054545170452457 1102 42-43 0.7903201503466946 1102 44-45 0.5297979842462794 1102 46-47 0.4905907013641979 1102 48-49 0.5688991908777439 1102 50-51 0.561868403022828 1102 52-53 0.5616935221527868 1102 54-55 0.5914543551354612 1102 56-57 0.5788656126729919 1102 58-59 0.6268692083706142 1102 60-61 0.5502245186729056 1102 62-63 0.6286274668288669 1102 64-65 0.4369916716464317 1102 66-67 0.5078711701285599 1102 68-69 0.5351327057776558 1102 70-71 0.4956939782623415 1102 72-73 0.6545015451713141 1102 74-75 0.7036211443716489 1102 76-77 0.5777217134027701 1102 78-79 0.6241070425055995 1102 80-81 0.7387691964644709 1102 82-83 0.6406618034469922 1102 84-85 0.6203501253614832 1102 86-87 0.7422135881355381 1102 88-89 0.8511859783243061 1102 90-91 1.0642150461206796 1102 92-93 1.0650782730345796 1102 94-95 1.2344786464563526 1102 96-97 1.1059795055208888 1102 98-99 1.466941847665936 1102 100-101 1.3885283069933223 1103 1 0.4831138485568296 1103 2 0.43623092383748485 1103 3 0.40057135169004 1103 4 0.2693161279688354 1103 5 0.25176130567851374 1103 6 0.25817928028305914 1103 7 0.3388364351058897 1103 8 0.3479164093443714 1103 9 0.4623677965098736 1103 10-11 0.4572430971086021 1103 12-13 0.3297103046305381 1103 14-15 0.4466570106467742 1103 16-17 0.4350799585719827 1103 18-19 0.5862104142787032 1103 20-21 0.5513163658409184 1103 22-23 0.5686071816145244 1103 24-25 0.6300424745099704 1103 26-27 0.5035272414466405 1103 28-29 0.5814967263073001 1103 30-31 0.6085975069398586 1103 32-33 0.6909786018707251 1103 34-35 0.7554087949959296 1103 36-37 0.6565729516052627 1103 38-39 0.6748199506600585 1103 40-41 0.7963497433643667 1103 42-43 0.710028358663422 1103 44-45 0.7688686152089801 1103 46-47 0.8967910731909257 1103 48-49 0.9102018443551572 1103 50-51 0.9263356931121365 1103 52-53 0.9196921186460685 1103 54-55 0.8257941942156535 1103 56-57 0.737845711592378 1103 58-59 0.8718309415695487 1103 60-61 0.7316235496965575 1103 62-63 0.7187778676633556 1103 64-65 0.7819593016246316 1103 66-67 0.8572540593599456 1103 68-69 0.9058403266140189 1103 70-71 1.0017174833372344 1103 72-73 0.9955002031962081 1103 74-75 0.8402606907371784 1103 76-77 0.9142051233087756 1103 78-79 0.9502057113717726 1103 80-81 1.0679211883305797 1103 82-83 1.0336471633190065 1103 84-85 1.0786360891222913 1103 86-87 1.1148894879620777 1103 88-89 1.0604564478397869 1103 90-91 1.1851125725499685 1103 92-93 1.1566504935638697 1103 94-95 1.2584785380435193 1103 96-97 1.1872221070773499 1103 98-99 1.569231189805123 1103 100-101 1.7745106005401183 1104 1 0.31861564775916307 1104 2 0.28235525818832485 1104 3 0.17015403979859656 1104 4 0.19119147611170462 1104 5 0.1683790002485992 1104 6 0.19205546118366357 1104 7 0.17578302720500005 1104 8 0.19282607323332002 1104 9 0.16239536942491384 1104 10-11 0.20338950371718312 1104 12-13 0.0686405799697738 1104 14-15 0.2294350096886859 1104 16-17 0.3122615958461026 1104 18-19 0.3614509461852933 1104 20-21 0.2978370260311962 1104 22-23 0.2782539419362564 1104 24-25 0.37364117162010757 1104 26-27 0.3759619507334193 1104 28-29 0.4058158284886204 1104 30-31 0.31162746393725627 1104 32-33 0.33034419505046486 1104 34-35 0.3694556981955017 1104 36-37 0.4169171465318513 1104 38-39 0.4579109301391213 1104 40-41 0.38852892274985606 1104 42-43 0.437457908472922 1104 44-45 0.3189059709385802 1104 46-47 0.4429741392433755 1104 48-49 0.5205756662396936 1104 50-51 0.5683973046515405 1104 52-53 0.6057783104644301 1104 54-55 0.5546887292081877 1104 56-57 0.4770504975819918 1104 58-59 0.46315438578870527 1104 60-61 0.5234005196281544 1104 62-63 0.612067205022143 1104 64-65 0.49352972535627515 1104 66-67 0.4749724772316526 1104 68-69 0.6057472680257519 1104 70-71 0.6846658634981075 1104 72-73 0.591688595834519 1104 74-75 0.5487963530998705 1104 76-77 0.6715780929395798 1104 78-79 0.6904262041749654 1104 80-81 0.8618898916794357 1104 82-83 0.9168147690430324 1104 84-85 0.8693896930972471 1104 86-87 0.8212670126941468 1104 88-89 0.9116086614713907 1104 90-91 0.9809187739492273 1104 92-93 0.9791686526957939 1104 94-95 1.1385408834680888 1104 96-97 1.0297149631857216 1104 98-99 1.0004002117743305 1104 100-101 0.9527301561427137 1105 1 0.3893774545913793 1105 2 0.3230351792608488 1105 3 0.27434663850525 1105 4 0.18349810595971405 1105 5 0.15727862071808119 1105 6 0.21555922743721823 1105 7 0.2703273299475697 1105 8 0.2571082040891781 1105 9 0.279661202481293 1105 10-11 0.3384482992398432 1105 12-13 0.26619674009440075 1105 14-15 0.3743288616661431 1105 16-17 0.427170345500393 1105 18-19 0.35900928103207974 1105 20-21 0.4741419174295558 1105 22-23 0.41570086460480127 1105 24-25 0.5694553128920532 1105 26-27 0.31063321408762334 1105 28-29 0.45526793131214305 1105 30-31 0.5183411964610372 1105 32-33 0.49820294701228107 1105 34-35 0.5171190837431894 1105 36-37 0.6425981084828436 1105 38-39 0.6039035978392562 1105 40-41 0.6092711902845096 1105 42-43 0.6968738743865615 1105 44-45 0.8783852030059975 1105 46-47 0.8096649159976863 1105 48-49 0.653391343761939 1105 50-51 0.6653421022347104 1105 52-53 0.6484940868950204 1105 54-55 0.6932222659140095 1105 56-57 0.6905429718812641 1105 58-59 0.5234191739823899 1105 60-61 0.6270979955227745 1105 62-63 0.6356317306886794 1105 64-65 0.6119968848935642 1105 66-67 0.6986844855825822 1105 68-69 0.7909003734459858 1105 70-71 0.8536712418682164 1105 72-73 0.8807030283532242 1105 74-75 0.8053811959836672 1105 76-77 0.7679657632277959 1105 78-79 0.8324540711643067 1105 80-81 0.8027166147981255 1105 82-83 0.8740476127263292 1105 84-85 0.926552597051888 1105 86-87 1.105642231618944 1105 88-89 0.9603235168822479 1105 90-91 0.9574990914188177 1105 92-93 0.9803713902559714 1105 94-95 1.2735749436013108 1105 96-97 1.3756175542260642 1105 98-99 1.4702848751356399 1105 100-101 1.2534846851261179 1106 1 0.0820748571796841 1106 2 0.0639429843614181 1106 3 0.08851004225079606 1106 4 0.06659088452887119 1106 5 0.08520404127703785 1106 6 0.18913204992492183 1106 7 0.24023641980551247 1106 8 0.1371354137814791 1106 9 0.16450971299165928 1106 10-11 -0.4603331110749167 1106 12-13 -0.06521203569907641 1106 14-15 0.0012921717379157371 1106 16-17 0.22283236360033953 1106 18-19 0.22239514518328463 1106 20-21 0.21466123365657097 1106 22-23 0.05429620413886482 1106 24-25 0.13055766696688664 1106 26-27 0.16869235917759084 1106 28-29 -0.014398912811394382 1106 30-31 0.12813486172287014 1106 32-33 0.04589164585384964 1106 34-35 -0.04077504110968988 1106 36-37 -0.23630825666021593 1106 38-39 0.0221662228319488 1106 40-41 0.24147295240033628 1106 42-43 0.11230487066875838 1106 44-45 0.22900369911516805 1106 46-47 0.3513082198614583 1106 48-49 0.3015875942376738 1106 50-51 0.3230816132386991 1106 52-53 0.4141790299913026 1106 54-55 0.0690615150646039 1106 56-57 0.12676769058713688 1106 58-59 0.28704156768292677 1106 60-61 0.0015689430529164383 1106 62-63 0.21822425349520103 1106 64-65 0.18973105753440933 1106 66-67 -0.01039996559606493 1106 68-69 -0.09712762250676121 1106 70-71 0.07420337146960776 1106 72-73 0.34244074209742337 1106 74-75 0.36014990301354644 1106 76-77 0.4407024430101991 1106 78-79 0.6472745104832569 1106 80-81 0.5281338840934673 1106 82-83 0.5193185118561807 1106 84-85 0.3151014009768076 1106 86-87 0.5313845091913514 1106 88-89 0.42121821547074134 1106 90-91 0.4989727011277978 1106 92-93 0.5485662922983074 1106 94-95 0.5052246460095517 1106 96-97 0.6916262597642024 1106 98-99 0.5842568111098103 1106 100-101 0.20112052146994586 1107 1 0.22866373577015864 1107 2 0.17551488515583458 1107 3 0.07787290459533835 1107 4 0.04697235838781921 1107 5 0.0973963050519231 1107 6 0.0995319109276096 1107 7 0.029738310706378 1107 8 0.16641015908329138 1107 9 0.12957663981995182 1107 10-11 0.1602440839919268 1107 12-13 0.12961481453070434 1107 14-15 0.13561449111797685 1107 16-17 0.1910583698464663 1107 18-19 0.14113484460160208 1107 20-21 0.2197196512747297 1107 22-23 0.364180043889462 1107 24-25 0.35930682870187525 1107 26-27 0.35186474771256115 1107 28-29 0.32161418630980876 1107 30-31 0.3250617493894197 1107 32-33 0.3598495472945089 1107 34-35 0.42556490792879487 1107 36-37 0.3911963041889166 1107 38-39 0.42613448969677137 1107 40-41 0.4027151408329317 1107 42-43 0.5275195602591438 1107 44-45 0.4761111770327844 1107 46-47 0.38353243926720637 1107 48-49 0.41937359338393776 1107 50-51 0.5871183725264757 1107 52-53 0.5365705649053112 1107 54-55 0.523794188994053 1107 56-57 0.5422704995952117 1107 58-59 0.49923026902683887 1107 60-61 0.43005400052992115 1107 62-63 0.5337620462004651 1107 64-65 0.5498893138431882 1107 66-67 0.4959956917380737 1107 68-69 0.48418822387371563 1107 70-71 0.5771265960821346 1107 72-73 0.5128659557339077 1107 74-75 0.38047716542283894 1107 76-77 0.49628222552691525 1107 78-79 0.5624444353159674 1107 80-81 0.6035096893122436 1107 82-83 0.5942265917621334 1107 84-85 0.6339511607627131 1107 86-87 0.8106125366483248 1107 88-89 0.6951294709401665 1107 90-91 0.7651016855037263 1107 92-93 0.9362126097759109 1107 94-95 0.9652743262969992 1107 96-97 1.0777465911827804 1107 98-99 0.8199941968229609 1107 100-101 0.8318131455503561 1108 1 0.31165048850908406 1108 2 0.26207125549406385 1108 3 0.2240870499592873 1108 4 0.21105775129563398 1108 5 0.271464072325287 1108 6 0.26637877512489894 1108 7 0.25542139546056575 1108 8 0.24830353740303224 1108 9 0.3119873292918669 1108 10-11 0.28702797859892115 1108 12-13 0.12307437552320266 1108 14-15 0.23383382665836905 1108 16-17 0.37821036717458867 1108 18-19 0.4610352341695716 1108 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-1.5700882882747393 2316 86-87 -1.5435575062950555 2316 88-89 -1.5861825726182417 2316 90-91 -1.475063900468065 2316 92-93 -1.6493631200868322 2316 94-95 -1.762692497525208 2316 96-97 -1.808515021538856 2316 98-99 -2.012121982791335 2316 100-101 -1.6999699065045313 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6730.0 3 1681.0 4 1805.0 5 2011.0 6 2296.0 7 2849.0 8 3502.0 9 4629.0 10 5815.0 11 7500.0 12 9752.0 13 12173.0 14 14566.0 15 17608.0 16 21500.0 17 27896.0 18 25098.0 19 20460.0 20 18744.0 21 19190.0 22 20106.0 23 22156.0 24 24409.0 25 26894.0 26 30594.0 27 35378.0 28 41506.0 29 49221.0 30 59136.0 31 71505.0 32 89546.0 33 117155.0 34 161750.0 35 237984.0 36 357173.0 37 469891.0 38 329235.0 39 35564.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.30229028812871 18.522201738566526 15.079373701548866 23.0961342717559 2 15.33089854990467 19.81177091181239 40.359593023134885 24.49773751514806 3 18.960451526097298 23.81150299844149 30.319581358156285 26.90846411730492 4 12.742483238064011 16.88572091927891 37.22381397392486 33.14798186873222 5 13.51060274847077 37.21480027748544 34.44011237577554 14.834484598268254 6 31.885733267175727 36.599546109965935 17.079152694201998 14.435567928656345 7 28.014670146401873 33.44673541806755 20.4635559875635 18.075038447967085 8 26.754504021949632 34.13537963585079 19.81228821580199 19.297828126397594 9 26.474106030381762 16.74969682724249 20.243370241019743 36.53282690135601 10-11 24.855667880912375 26.409594455249763 27.202599268123596 21.53213839571427 12-13 26.28840542820376 23.87021861875637 27.389418445095142 22.45195750794473 14-15 22.606620874434704 25.238412221616652 26.154867054139398 26.000099849809246 16-17 21.884562849203267 28.28889202319777 27.322325252225188 22.504219875373778 18-19 21.982270111403647 26.44910863769351 29.94422328659062 21.624397964312223 20-21 22.16554667971619 25.645433311986377 30.519488355506287 21.669531652791147 22-23 22.011148661650914 25.564573926194985 30.534226640911783 21.89005077124232 24-25 22.051589484837613 25.754498608737777 30.420179503207436 21.773732403217174 26-27 21.346521478898072 26.15996992556911 30.175060399551377 22.318448195981436 28-29 21.671241147064567 26.822945868290333 29.882379717309387 21.623433267335713 30-31 21.687534068165455 26.112505045209232 29.751588460722267 22.44837242590304 32-33 21.219313431426052 26.179824534941034 29.861703041750815 22.739158991882107 34-35 22.417747215034517 26.334206676760086 29.435529424835423 21.812516683369974 36-37 22.64459907624524 25.57464215997422 29.500464665163346 22.280294098617198 38-39 21.821558786764477 26.18914011526694 29.33891018454774 22.65039091342084 40-41 22.43170715067773 25.999308626938088 29.521629743518062 22.047354478866115 42-43 22.110070619527082 25.978909194224393 29.25155947209132 22.659460714157206 44-45 21.992609137494952 26.30435456177085 29.561474520946636 22.141561779787565 46-47 22.747735148306376 26.7778121375629 28.47387998942726 22.000572724703463 48-49 22.24730933912465 26.870672966243443 28.2571746277537 22.624843066878206 50-51 22.45570976977253 27.087272824823614 28.347078272431993 22.109939132971864 52-53 23.022880139213996 26.533820282853288 28.295622611626563 22.147676966306154 54-55 22.217557116182558 26.901850397327703 28.52865536186008 22.351937124629657 56-57 22.398418043191466 27.07655745968581 28.219936748345926 22.30508774877681 58-59 22.427569186850974 26.507662612961624 28.192089972538614 22.872678227648787 60-61 21.756057531078167 26.941079120517593 28.722865548628384 22.57999779977586 62-63 22.035148439085578 26.92307052478532 28.89698347423651 22.14479756189259 64-65 21.640953658359066 27.0360481196128 28.557555340673968 22.76544288135417 66-67 21.568704917044244 26.973149324522428 28.56836598262909 22.889779775804243 68-69 22.390861616301503 26.5311562840112 28.70227931424693 22.375702785440367 70-71 22.16791431571206 26.889693292978166 27.99510646475773 22.94728592655205 72-73 22.047644331969195 26.996596833430768 28.19120958518721 22.76454924941282 74-75 22.172138707719157 27.460162011873773 28.015438773666073 22.352260506740997 76-77 22.414173148441588 26.95451403531975 28.06217596324124 22.56913685299742 78-79 22.41785030854154 27.363684774243907 27.88295611842123 22.33550879879332 80-81 22.843171258752076 27.394771294018227 27.464970897107676 22.297086550122017 82-83 22.658714999957372 27.562635595305107 27.585114809310348 22.193534595427174 84-85 22.553071591804652 27.803733362167737 27.543142437103224 22.100052608924386 86-87 23.01165555059783 27.72810119191925 27.00597602189309 22.25426723558983 88-89 22.62379866088959 27.903742287802245 26.87967141212254 22.59278763918562 90-91 22.526013010769642 27.856896510838915 26.99010817069178 22.626982307699667 92-93 22.94970071910188 27.285899409757054 27.384103391614477 22.38029647952659 94-95 22.592625412543313 27.03517554490248 27.639039525528908 22.733159517025303 96-97 22.982451250700937 26.567494421639214 27.61635936457437 22.83369496308548 98-99 22.639434815341676 27.026957328308733 27.095879243148364 23.23772861320123 100-101 23.07853143641962 26.300278955857497 27.559734976316346 23.061454631406537 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 720.0 1 492.0 2 428.0 3 606.5 4 513.5 5 491.5 6 938.0 7 2214.0 8 3785.0 9 4369.5 10 3556.0 11 2611.5 12 2435.5 13 2516.5 14 2722.5 15 2921.0 16 3110.0 17 3358.0 18 3683.0 19 4127.5 20 4714.0 21 5592.0 22 6884.5 23 8429.0 24 10541.5 25 13507.5 26 17107.0 27 21132.5 28 24292.5 29 27411.0 30 31379.5 31 37405.0 32 46067.0 33 48580.0 34 46275.5 35 47550.0 36 51207.0 37 54935.0 38 57969.0 39 60688.5 40 64730.0 41 69922.5 42 72543.5 43 74097.0 44 76546.0 45 82196.0 46 85371.0 47 86814.0 48 88848.0 49 86236.5 50 83323.0 51 81220.0 52 79971.0 53 81044.0 54 84244.0 55 80990.5 56 73625.0 57 67925.5 58 63614.5 59 56184.5 60 43397.5 61 36066.0 62 32251.5 63 23576.0 64 15334.5 65 11657.0 66 8907.0 67 6934.0 68 5454.0 69 4272.5 70 3296.0 71 2205.0 72 1505.5 73 1136.0 74 832.5 75 622.0 76 443.5 77 265.5 78 154.5 79 101.0 80 67.0 81 42.0 82 27.0 83 16.0 84 20.0 85 16.5 86 9.5 87 10.0 88 7.5 89 6.0 90 4.0 91 5.5 92 4.5 93 1.5 94 1.0 95 2.5 96 2.0 97 0.5 98 0.5 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4896027502576911 2 0.0514758560355857 3 0.05941760765335377 4 0.06856517496177772 5 0.08324286250665797 6 0.11089346368893947 7 0.0733052780215974 8 0.05513488295895528 9 0.05264009187483966 10-11 0.07787906167580938 12-13 0.10945895881557298 14-15 0.058253371814099814 16-17 0.0625360965084983 18-19 0.06232819725148866 20-21 0.07725536390478047 22-23 0.0658416946949515 24-25 0.08968773947395667 26-27 0.06821174622486134 28-29 0.06825332607626326 30-31 0.07239052129075502 32-33 0.07236973136505405 34-35 0.07461504334075811 36-37 0.06933440221271336 38-39 0.042660927538377164 40-41 0.045696256690717835 42-43 0.04459439062856677 44-45 0.023097607453770482 46-47 0.029708803826676886 48-49 0.0300414426378923 50-51 0.027920870216394018 52-53 0.027234802668262226 54-55 0.026423995565924643 56-57 0.02690216385704681 58-59 0.02261943916264832 60-61 0.029542484421069177 62-63 0.021746262283207852 64-65 0.024365792921529256 66-67 0.017297218183201655 68-69 0.019625689861709573 70-71 0.042536187984171386 72-73 0.03852373232388542 74-75 0.03345099045285032 76-77 0.02399157425891191 78-79 0.016278511823854445 80-81 0.01825355476544598 82-83 0.023596565670593605 84-85 0.01979200926731728 86-87 0.009729685228050932 88-89 0.04270250738977909 90-91 0.05446960533652445 92-93 0.03457364644070235 94-95 0.05505172325615142 96-97 0.044864659662679295 98-99 0.034968655029020655 100-101 0.1702071217137884 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2405011.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.612081336376654 #Duplication Level Percentage of deduplicated Percentage of total 1 73.66252657588153 37.28213786501523 2 14.25663501030524 14.431159414492079 3 5.145052088537289 7.812053843548316 4 2.445015436039712 4.949892804701534 5 1.2994067828694391 3.2882840891813787 6 0.754443436527461 2.2910371543934027 7 0.5110516977960811 1.8105773067164048 8 0.3600113491814988 1.4576738949434171 9 0.2436719414040803 1.1099469705962506 >10 1.1861042038929617 10.558103102402882 >50 0.08099877486728851 2.858973484352604 >100 0.04575765136628726 4.565215785529804 >500 0.004373696642067922 1.491645008718644 >1k 0.0047037869546758294 4.928685134648144 >5k 1.6504515630441508E-4 0.659867264734895 >10k+ 8.252257815220754E-5 0.5047468760251412 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12085 0.5024925041922885 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9480 0.3941769912902685 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6319 0.2627430810087771 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 4775 0.1985437904442017 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 4649 0.19330472916755892 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 4590 0.1908515179348452 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 4390 0.18253554765445978 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 4018 0.16706784293294294 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 3618 0.1504359023721721 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 3580 0.14885586801889888 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 3251 0.13517609690766488 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 3163 0.1315170699842953 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 3018 0.12548799153101586 No Hit GGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCT 2707 0.11255665774501655 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 2557 0.1063196800347275 No Hit GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA 2515 0.10457332627584655 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.247395542057812E-4 2 0.0 0.0 0.0 0.0 1.247395542057812E-4 3 0.0 0.0 0.0 0.0 1.247395542057812E-4 4 0.0 0.0 0.0 0.0 1.247395542057812E-4 5 0.0 0.0 0.0 0.0 1.247395542057812E-4 6 0.0 0.0 0.0 0.0 1.247395542057812E-4 7 0.0 0.0 0.0 8.315970280385412E-5 1.247395542057812E-4 8 0.0 0.0 0.0 8.315970280385412E-5 1.247395542057812E-4 9 0.0 0.0 0.0 1.6631940560770823E-4 1.247395542057812E-4 10-11 0.0 0.0 0.0 1.6631940560770823E-4 1.455294799067447E-4 12-13 0.0 0.0 0.0 1.6631940560770823E-4 5.821179196269788E-4 14-15 0.0 0.0 0.0 1.8710933130867177E-4 7.0685747383276E-4 16-17 0.0 0.0 0.0 2.2868918271059884E-4 8.523869537395048E-4 18-19 0.0 0.0 0.0 2.494791084115624E-4 8.939668051414318E-4 20-21 0.0 0.0 0.0 3.5342873691638E-4 9.979164336462495E-4 22-23 0.0 0.0 0.0 4.573783654211977E-4 0.0011642358392539576 24-25 0.0 0.0 0.0 6.860675481317965E-4 0.0012473955420578117 26-27 0.0 0.0 0.0 0.0012266056163568483 0.0012473955420578117 28-29 0.0 0.0 0.0 0.003950085883183071 0.0012681854677587753 30-31 0.0 0.0 0.0 0.011995787129455957 0.001288975393459739 32-33 0.0 0.0 0.0 0.02891878665004027 0.001288975393459739 34-35 0.0 0.0 0.0 0.05374195793699073 0.00141371494766552 36-37 0.0 0.0 0.0 0.09174594211835206 0.00141371494766552 38-39 0.0 0.0 0.0 0.1460492280492688 0.00141371494766552 40-41 0.0 0.0 0.0 0.20685976072458712 0.00141371494766552 42-43 0.0 0.0 0.0 0.26758713369710163 0.0014760847247684106 44-45 0.0 0.0 0.0 0.34598594351543505 0.0015592444275722647 46-47 0.0 0.0 0.0 0.43380258967630503 0.0016008242789741917 48-49 0.0 0.0 0.0 0.5246961448409175 0.0016216142046751553 50-51 0.0 0.0 0.0 0.6271488986952658 0.0017463537588809366 52-53 0.0 0.0 0.0 0.722242018851473 0.0018710933130867177 54-55 0.0 0.0 0.0 0.8244037137460078 0.001954253015890572 56-57 0.0 0.0 0.0 0.9424073320246769 0.0020166227929934626 58-59 0.0 0.0 0.0 1.0640283973753135 0.0020997824957973165 60-61 0.0 0.0 0.0 1.1998905618311102 0.0021413623471992437 62-63 0.0 0.0 0.0 1.331116572855592 0.0021621522729002073 64-65 0.0 0.0 0.0 1.4729246560618643 0.0021621522729002073 66-67 0.0 0.0 0.0 1.632632865296666 0.0021621522729002073 68-69 0.0 0.0 0.0 1.8054803075744768 0.0021621522729002073 70-71 0.0 0.0 0.0 1.9827144241751908 0.0022245220500030976 72-73 0.0 0.0 0.0 2.170239553997882 0.0024116313813117695 74-75 0.0 0.0 0.0 2.3552906826621585 0.002577950786919478 76-77 0.0 0.0 0.0 2.5513396820222445 0.0026403205640223686 78-79 0.0 0.0 0.0 2.7747898034562004 0.0027234802668262225 80-81 0.0 0.0 0.0 3.0068261642046545 0.0027858500439291133 82-83 0.0 0.0 0.0 3.2529580945783616 0.0031184888551445294 84-85 0.0 0.0 0.0 3.5059714903590877 0.0031184888551445294 86-87 0.0 0.0 0.0 3.75761275104355 0.0031184888551445294 88-89 0.0 0.0 0.0 4.0221229757369095 0.0031184888551445294 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6275 0.0 47.2338 1 GTATCAA 10330 0.0 38.25648 1 TATCAAC 13080 0.0 29.791782 2 TAAGGTG 1980 0.0 29.520947 5 ATCAACG 13180 0.0 29.493631 3 TCAACGC 13355 0.0 29.213905 4 CAACGCA 13450 0.0 29.007563 5 AACGCAG 13785 0.0 28.268156 6 AGGTAAG 1960 0.0 25.942875 2 GTAAGGT 2220 0.0 25.259188 4 ACGCAGA 15340 0.0 25.134298 7 CGCAGAG 15435 0.0 24.887196 8 GGTAAGG 2160 0.0 24.64079 3 AAGGTAA 2030 0.0 23.981653 1 TAAGGTA 1110 0.0 23.118578 4 TAAGCGA 165 2.4221627E-6 23.040741 4 GTGTAAG 1145 0.0 22.926207 1 GAGTACT 11180 0.0 22.76851 12-13 GCAGAGT 17005 0.0 22.22602 9 GTACATG 7295 0.0 22.113955 1 >>END_MODULE