FastQCFastQC Report
Thu 26 May 2016
SRR937997_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937997_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2405011
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113790.47313712910252803No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97130.40386509666691756No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63520.2641152161050407No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC50090.20827347567225263No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA48990.20369969201804067No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA43390.18041497523296152No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA43340.18020707597595187No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA43150.17941705879931524No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG38930.16187036150770204No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA35070.14582053886655819No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA32420.13480187824504752No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA32150.1336792222571955No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT29650.12328425940671374No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA27860.11584146600576879No Hit
GGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCT27080.11259823759641847No Hit
GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA24700.10270223296275985No Hit
CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG24480.10178747623191743No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61100.046.346011
GTATCAA102150.037.489011
TATCAAC129100.029.4790532
ATCAACG129350.029.2672443
TCAACGC132150.028.645344
CAACGCA133350.028.4231825
TAAGGTG19750.028.3777375
AACGCAG136900.027.6861296
GTAAGGT21200.026.8848864
AAGGTAA19250.025.6690561
AGGTAAG19500.025.583622
ACGCAGA152850.024.7038427
CGCAGAG153400.024.5533438
GGTAAGG22650.023.697373
GAGTACT110700.022.37533212-13
ATACCGA1503.0366544E-522.1650946
GTGTAAG10400.021.9288161
GCAGAGT169750.021.8246679
TAAGGTA9900.021.5893764
GTACTTT115600.021.20091814-15
Produced by FastQC (version 0.11.5)