##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937997_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2405011 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.510190182082326 34.0 31.0 34.0 31.0 34.0 2 32.72965487475941 34.0 31.0 34.0 31.0 34.0 3 32.785432166422524 34.0 31.0 34.0 31.0 34.0 4 36.24492029350385 37.0 35.0 37.0 35.0 37.0 5 36.20913251540222 37.0 37.0 37.0 35.0 37.0 6 36.24951819347188 37.0 37.0 37.0 35.0 37.0 7 36.215250574737496 37.0 37.0 37.0 35.0 37.0 8 36.203535451605006 37.0 37.0 37.0 35.0 37.0 9 37.919760865958615 39.0 38.0 39.0 35.0 39.0 10-11 37.90340647090595 39.0 38.0 39.0 35.0 39.0 12-13 37.857555952966536 39.0 38.0 39.0 35.0 39.0 14-15 39.18044221003563 40.5 39.0 41.0 36.0 41.0 16-17 39.136610393881774 40.0 38.5 41.0 36.0 41.0 18-19 39.17170254106946 40.0 39.0 41.0 36.0 41.0 20-21 39.10229433462051 40.0 39.0 41.0 36.0 41.0 22-23 38.9627051601843 40.0 38.5 41.0 35.0 41.0 24-25 38.9079486538731 40.0 38.0 41.0 35.0 41.0 26-27 38.86118026071398 40.0 38.0 41.0 35.0 41.0 28-29 38.80126910022449 40.0 38.0 41.0 35.0 41.0 30-31 38.584916243626324 40.0 38.0 41.0 34.5 41.0 32-33 38.386399895884054 40.0 38.0 41.0 34.0 41.0 34-35 38.17105514278313 40.0 38.0 41.0 34.0 41.0 36-37 38.012255453301464 40.0 38.0 41.0 33.5 41.0 38-39 37.96433550615777 40.0 38.0 41.0 33.0 41.0 40-41 37.887569952902496 40.0 38.0 41.0 33.0 41.0 42-43 37.72688690405158 40.0 37.5 41.0 33.0 41.0 44-45 37.51240119068062 40.0 37.0 41.0 33.0 41.0 46-47 37.17243268326008 40.0 37.0 41.0 32.0 41.0 48-49 36.80172481539586 40.0 36.0 41.0 31.0 41.0 50-51 36.48325413064639 40.0 36.0 41.0 30.5 41.0 52-53 36.05654755841034 39.0 35.0 41.0 29.5 41.0 54-55 35.818272556757535 39.0 35.0 41.0 29.0 41.0 56-57 35.55527355176338 39.0 35.0 40.0 28.0 41.0 58-59 35.21049758192375 38.0 35.0 40.0 27.5 41.0 60-61 34.849111708844575 38.0 34.0 40.0 27.0 41.0 62-63 34.483915458182935 37.0 34.0 40.0 26.0 41.0 64-65 34.08585823515983 37.0 34.0 39.5 26.0 41.0 66-67 33.66356162196348 36.0 33.5 39.0 25.0 41.0 68-69 33.2025537513134 35.5 33.0 39.0 23.5 40.0 70-71 32.672843700091185 35.0 32.5 38.0 22.5 40.0 72-73 32.260232905379645 35.0 32.0 37.5 21.5 39.5 74-75 31.825899341000934 35.0 31.5 37.0 20.0 39.0 76-77 30.52656474336292 33.5 30.0 35.5 19.5 38.0 78-79 31.24078351408788 34.5 31.0 36.0 20.0 38.5 80-81 31.08468256486145 35.0 31.0 36.0 18.5 37.0 82-83 30.71684578573653 34.5 31.0 35.0 17.5 37.0 84-85 30.342103009092266 34.0 31.0 35.0 13.0 36.5 86-87 29.928614255818374 34.0 30.5 35.0 7.0 36.0 88-89 29.453054892472426 34.0 30.0 35.0 4.5 36.0 90-91 29.114776606011365 34.0 29.5 35.0 2.0 35.0 92-93 28.71526055390183 34.0 29.0 35.0 2.0 35.0 94-95 28.298523374737165 34.0 29.0 35.0 2.0 35.0 96-97 27.71779546954255 34.0 28.0 35.0 2.0 35.0 98-99 27.018707814642013 33.0 26.5 35.0 2.0 35.0 100-101 24.52979695311165 31.0 20.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12925788196117338 1101 2 0.1457182503724468 1101 3 0.09545177982634812 1101 4 0.005785007682710841 1101 5 0.09657718443401819 1101 6 0.043855623712964587 1101 7 0.1155452394111336 1101 8 0.14002230784615222 1101 9 0.08635276681200565 1101 10-11 0.09660731942496881 1101 12-13 0.18429091277837983 1101 14-15 0.10601208681432439 1101 16-17 0.19537042980530117 1101 18-19 0.19143794317059104 1101 20-21 0.24273626089993883 1101 22-23 0.28969115218770725 1101 24-25 0.21411887521096418 1101 26-27 0.18038954958989706 1101 28-29 0.15735300948112751 1101 30-31 0.17446955076349724 1101 32-33 0.22985330925818204 1101 34-35 0.2217244810478718 1101 36-37 0.25893292681603697 1101 38-39 0.2683023484514777 1101 40-41 0.32173352723748394 1101 42-43 0.39790031203319387 1101 44-45 0.450581247215446 1101 46-47 0.4430014368890909 1101 48-49 0.360616227129988 1101 50-51 0.3053010979031825 1101 52-53 0.2265487113314677 1101 54-55 0.376599308401957 1101 56-57 0.3556505409201449 1101 58-59 0.2561866082342732 1101 60-61 0.40452079040969124 1101 62-63 0.4551777648435049 1101 64-65 0.543287024424508 1101 66-67 0.29838033371343897 1101 68-69 0.40927426535755274 1101 70-71 0.41602746006338265 1101 72-73 0.44143434976790985 1101 74-75 0.47504480870709287 1101 76-77 0.4424451146370991 1101 78-79 0.38647838759573716 1101 80-81 0.4719241001613774 1101 82-83 0.4895203798770247 1101 84-85 0.5475270690564251 1101 86-87 0.6474071825139092 1101 88-89 0.5244389890480505 1101 90-91 0.5439371982820553 1101 92-93 0.6138355839374867 1101 94-95 0.49012283071768437 1101 96-97 0.589081091181896 1101 98-99 0.5814365257868062 1101 100-101 0.7409780747637313 1102 1 0.11315564316988969 1102 2 0.15809699070989325 1102 3 0.12343667089887589 1102 4 0.07708357020040069 1102 5 0.11202137812253454 1102 6 0.058485491316865534 1102 7 0.046271139975559095 1102 8 0.06717834210839158 1102 9 0.0940472401413075 1102 10-11 0.09739913045287807 1102 12-13 0.1444697239531152 1102 14-15 0.1626942150812738 1102 16-17 0.20476829015594689 1102 18-19 0.15575820036443133 1102 20-21 0.1182811631126981 1102 22-23 0.12724236921166465 1102 24-25 0.21696655687909328 1102 26-27 0.08837032362348651 1102 28-29 0.17164534043656943 1102 30-31 0.1758389959773652 1102 32-33 0.05606885743737422 1102 34-35 0.15225531418821703 1102 36-37 0.13798090696948861 1102 38-39 0.13975783523834906 1102 40-41 0.2485841892179863 1102 42-43 0.04333584783099553 1102 44-45 0.08983895417612331 1102 46-47 0.08221127570399034 1102 48-49 0.17344874992127757 1102 50-51 0.046653802510178366 1102 52-53 -0.008847909366643592 1102 54-55 -0.05886307896599874 1102 56-57 0.040908779160467645 1102 58-59 0.0011347214346812962 1102 60-61 0.07951859566466624 1102 62-63 -0.09037643854730248 1102 64-65 -0.06326065678953086 1102 66-67 -0.10768647129784625 1102 68-69 0.033308955951476094 1102 70-71 0.10309257890503432 1102 72-73 -0.017242953087226454 1102 74-75 0.05887744653329818 1102 76-77 0.011170906221082078 1102 78-79 0.12692462466755572 1102 80-81 0.20149670005633524 1102 82-83 0.25964846216936266 1102 84-85 0.12163225637413078 1102 86-87 0.05209016294737623 1102 88-89 3.6736619303567863E-4 1102 90-91 0.045242882368945914 1102 92-93 0.12760174369373445 1102 94-95 -0.03364928512247545 1102 96-97 0.19765039744569535 1102 98-99 0.05876632331022691 1102 100-101 0.13522863745094682 1103 1 0.19109385078339614 1103 2 0.17966638371520816 1103 3 0.1644225830903423 1103 4 0.08371396740159298 1103 5 0.10088122669617405 1103 6 0.13289918297903824 1103 7 0.13844878759282153 1103 8 0.1024275304771578 1103 9 0.13686261894162044 1103 10-11 0.10570992661330791 1103 12-13 0.11968237426604134 1103 14-15 0.1897139169166806 1103 16-17 0.2001373040093526 1103 18-19 0.15801344373659987 1103 20-21 0.25155917904059066 1103 22-23 0.17246975048495727 1103 24-25 0.2134876402456385 1103 26-27 0.19336247834325349 1103 28-29 0.2278711306782384 1103 30-31 0.20155701126064685 1103 32-33 0.26089916204814756 1103 34-35 0.26799956830790705 1103 36-37 0.2351766771108359 1103 38-39 0.1403101546870218 1103 40-41 0.31591606874496136 1103 42-43 0.33517258102993 1103 44-45 0.4116016589884808 1103 46-47 0.39897270593300505 1103 48-49 0.38715389044775605 1103 50-51 0.3003997223784438 1103 52-53 0.3486025796631438 1103 54-55 0.3593938257596321 1103 56-57 0.4394046975636954 1103 58-59 0.38379961882340297 1103 60-61 0.4811802787592967 1103 62-63 0.5210366128931199 1103 64-65 0.5080517318675604 1103 66-67 0.39618306117912283 1103 68-69 0.489203794035447 1103 70-71 0.48303049058080916 1103 72-73 0.3367363107603154 1103 74-75 0.43601768439691924 1103 76-77 0.4671074978973202 1103 78-79 0.25091026875699995 1103 80-81 0.44405174154622173 1103 82-83 0.4737918322849275 1103 84-85 0.4799709248887005 1103 86-87 0.5793907858816745 1103 88-89 0.49908014689437863 1103 90-91 0.3646668366245862 1103 92-93 0.499893803479015 1103 94-95 0.3347413776022634 1103 96-97 0.4095269870122813 1103 98-99 0.639017093920387 1103 100-101 0.8306491840274433 1104 1 0.13857197348565364 1104 2 0.1357394575103612 1104 3 0.10314943855256331 1104 4 0.04975924547026267 1104 5 0.03764177124732271 1104 6 0.025385702887241735 1104 7 0.065493012877468 1104 8 0.05772703082251951 1104 9 0.10265305643186906 1104 10-11 0.07221893098041221 1104 12-13 0.1406297824120344 1104 14-15 0.2382993428207314 1104 16-17 0.1383976480302067 1104 18-19 0.17989091555094916 1104 20-21 0.1960361361605365 1104 22-23 0.15324303845759601 1104 24-25 0.109830364616748 1104 26-27 0.1397197550861975 1104 28-29 0.13381006502685722 1104 30-31 0.2021801869920239 1104 32-33 0.21286206115913586 1104 34-35 0.26479212807707597 1104 36-37 0.2138069716675588 1104 38-39 0.18162914267748675 1104 40-41 0.27578964414434637 1104 42-43 0.26523520496879627 1104 44-45 0.3653494865769886 1104 46-47 0.28926209620951226 1104 48-49 0.33940261485228973 1104 50-51 0.30736066492651304 1104 52-53 0.2826523097827973 1104 54-55 0.2814214864066713 1104 56-57 0.4645757749683881 1104 58-59 0.41037771562382375 1104 60-61 0.330384110837457 1104 62-63 0.4120311665461216 1104 64-65 0.4484613358759333 1104 66-67 0.3719798209438352 1104 68-69 0.36499843012867217 1104 70-71 0.27399089141857047 1104 72-73 0.32881153468580493 1104 74-75 0.40278065898527515 1104 76-77 0.32118567825203925 1104 78-79 0.38588508410202493 1104 80-81 0.385740248375388 1104 82-83 0.42108491705353757 1104 84-85 0.5272349745422282 1104 86-87 0.3951594992570442 1104 88-89 0.36075898231471015 1104 90-91 0.43972278318326374 1104 92-93 0.6200126664165602 1104 94-95 0.599424477076429 1104 96-97 0.6348751646932591 1104 98-99 0.8889331914895138 1104 100-101 0.9597685844975778 1105 1 0.07263954392922045 1105 2 0.07043968949179202 1105 3 0.08454427546334387 1105 4 0.07146496472392272 1105 5 0.009250026426229851 1105 6 0.07479937455200769 1105 7 0.0693309824638888 1105 8 0.1001847457572822 1105 9 0.08413770438751556 1105 10-11 0.11196172941288296 1105 12-13 0.1233599153290541 1105 14-15 0.1947991723291551 1105 16-17 0.16159072783188577 1105 18-19 0.10384229274770718 1105 20-21 0.12186433178194278 1105 22-23 0.11300636228302352 1105 24-25 0.1295941067856745 1105 26-27 0.0905788367425302 1105 28-29 0.0398540163276877 1105 30-31 0.1155019040995171 1105 32-33 0.1755507183063827 1105 34-35 0.23352136653586086 1105 36-37 0.1798283490203616 1105 38-39 0.15887490852664854 1105 40-41 0.27082065302617764 1105 42-43 0.1685406664431781 1105 44-45 0.16252244737653854 1105 46-47 0.08998425337452431 1105 48-49 0.14471476384633064 1105 50-51 0.16571860360864576 1105 52-53 0.3139429924430246 1105 54-55 0.4016362261090265 1105 56-57 0.19359858763782256 1105 58-59 0.25355202641121366 1105 60-61 0.2378037814151952 1105 62-63 0.3205288186762303 1105 64-65 0.16471271914863195 1105 66-67 0.33704324148629183 1105 68-69 0.12676990235581087 1105 70-71 0.22705914216835765 1105 72-73 0.09697536198431322 1105 74-75 0.1371974466450716 1105 76-77 0.20553411987269854 1105 78-79 0.14394583289040597 1105 80-81 0.1927926731241918 1105 82-83 0.41920892867551984 1105 84-85 0.40270897266765004 1105 86-87 0.3591470133636889 1105 88-89 0.06768304865336816 1105 90-91 0.18893988320620636 1105 92-93 0.36358387229833866 1105 94-95 0.18810242529413657 1105 96-97 0.4006833196689392 1105 98-99 0.35197619554113757 1105 100-101 0.3881958221123689 1106 1 -0.10974763592166425 1106 2 0.0033727161596672772 1106 3 0.03270271117637691 1106 4 0.0218159075443225 1106 5 -0.04280845975533509 1106 6 -0.028575145170378846 1106 7 0.03978302847543347 1106 8 -0.04491939059474248 1106 9 -0.05975186477986938 1106 10-11 -0.001553074610718852 1106 12-13 -0.05315368080439242 1106 14-15 -0.14509048412812575 1106 16-17 -0.13153051929312198 1106 18-19 -0.040155411658425066 1106 20-21 -0.01729537924291691 1106 22-23 0.0322580827162966 1106 24-25 -0.007067582511488979 1106 26-27 -0.02887151705024138 1106 28-29 0.04227932362149289 1106 30-31 0.09817665202959347 1106 32-33 0.09132297572231352 1106 34-35 -0.01913271030107211 1106 36-37 -0.12263601072864105 1106 38-39 -0.04154919657689504 1106 40-41 -0.04609645365916748 1106 42-43 0.00546938789543816 1106 44-45 0.08347990348853074 1106 46-47 0.0379768220082326 1106 48-49 0.13044221516552312 1106 50-51 0.03602838012535159 1106 52-53 -0.1357282780597231 1106 54-55 -0.4505598393642529 1106 56-57 -0.1855459518827587 1106 58-59 -0.12611617103135586 1106 60-61 -0.23524196321073987 1106 62-63 -0.10028369713081275 1106 64-65 -0.24823312089361593 1106 66-67 -0.268169821576258 1106 68-69 -0.42899783422544857 1106 70-71 -0.35552352795451014 1106 72-73 -0.3135187069444072 1106 74-75 -0.24898703891211582 1106 76-77 -0.06634457844610964 1106 78-79 -0.06166461088912101 1106 80-81 -0.3943491024336261 1106 82-83 -0.31876106672710236 1106 84-85 -0.26467419852525254 1106 86-87 0.011005775062120193 1106 88-89 -0.0012862254676733187 1106 90-91 -0.15892588708881306 1106 92-93 0.06552244073434821 1106 94-95 -0.1009940412729371 1106 96-97 0.006599098929090275 1106 98-99 -0.11608889535841982 1106 100-101 -0.06021292062168371 1107 1 0.09638633898256899 1107 2 0.09265551755538581 1107 3 0.05194921327539248 1107 4 -0.0034143848687619993 1107 5 0.032927260365084976 1107 6 0.02409399061511408 1107 7 -0.03141981071955513 1107 8 -0.03781890316668779 1107 9 -0.014661175911967916 1107 10-11 0.014620987016833453 1107 12-13 0.034175330321019715 1107 14-15 0.13799653106095633 1107 16-17 0.04869770753497704 1107 18-19 0.04998463875309511 1107 20-21 0.015311254628549875 1107 22-23 0.025293920771936484 1107 24-25 0.08066665513765514 1107 26-27 0.015504498164467861 1107 28-29 0.029913235089452428 1107 30-31 0.04706147051123821 1107 32-33 0.0672043805201028 1107 34-35 0.1373243364686445 1107 36-37 0.15604194926088866 1107 38-39 0.18449798625893976 1107 40-41 0.2107271641332389 1107 42-43 0.12821622842539426 1107 44-45 0.07643597746460529 1107 46-47 0.11227983551235354 1107 48-49 0.1426313540009545 1107 50-51 0.10441689884780203 1107 52-53 0.18039934696754756 1107 54-55 0.20478504145368248 1107 56-57 0.20955943982527714 1107 58-59 0.19893620295389525 1107 60-61 0.20914934858259215 1107 62-63 0.26586428687699737 1107 64-65 0.30140443300377484 1107 66-67 0.21427083113609768 1107 68-69 0.41650828409350993 1107 70-71 0.33530214979798245 1107 72-73 0.2436501072848145 1107 74-75 0.278371884133751 1107 76-77 0.1600311136498611 1107 78-79 0.3468954420098562 1107 80-81 0.39237417042795997 1107 82-83 0.388972408436814 1107 84-85 0.5426259412845376 1107 86-87 0.5618168218480903 1107 88-89 0.4683363162205829 1107 90-91 0.4252638879842934 1107 92-93 0.5583721886454818 1107 94-95 0.5804221312887172 1107 96-97 0.8081361472737782 1107 98-99 0.983839481029289 1107 100-101 0.9176995385744178 1108 1 0.04915000433442884 1108 2 0.029761500519711603 1108 3 0.05827542646999717 1108 4 0.027673650665299476 1108 5 0.01241460550085094 1108 6 0.0307218873869175 1108 7 0.09042012999200466 1108 8 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-0.7678847625067888 2316 50-51 -0.6137787177094793 2316 52-53 -0.5660494780308909 2316 54-55 -0.5962938256978632 2316 56-57 -0.6080360775482063 2316 58-59 -0.6809569883534365 2316 60-61 -0.40039928211410114 2316 62-63 -0.5642218318011061 2316 64-65 -0.5598721832711817 2316 66-67 -0.3929140956757706 2316 68-69 -0.4223097451382287 2316 70-71 -0.5451891077689339 2316 72-73 -0.4406757750472394 2316 74-75 -0.38920513649270205 2316 76-77 -0.37768557173307116 2316 78-79 -0.3725225060194397 2316 80-81 -0.46032522364609463 2316 82-83 -0.387535921112228 2316 84-85 -0.33750096472506996 2316 86-87 -0.2573068961927696 2316 88-89 -0.4223066060821985 2316 90-91 -0.4284086611824094 2316 92-93 -0.5955906821412817 2316 94-95 -0.5399049347206173 2316 96-97 -0.487009158131567 2316 98-99 -0.3671274178106856 2316 100-101 -0.39433099193863086 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 2.0 4 7.0 5 31.0 6 83.0 7 186.0 8 561.0 9 1022.0 10 1923.0 11 2965.0 12 4618.0 13 6767.0 14 9429.0 15 12762.0 16 15755.0 17 22534.0 18 22472.0 19 16212.0 20 13434.0 21 12728.0 22 13163.0 23 14151.0 24 16071.0 25 18905.0 26 21727.0 27 26065.0 28 31612.0 29 38859.0 30 47384.0 31 59055.0 32 73521.0 33 97149.0 34 136539.0 35 210646.0 36 353315.0 37 569756.0 38 482239.0 39 51357.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.706582215216486 18.905568415279596 15.416977302806515 22.97087206669741 2 15.528184330754701 20.11748981341859 39.733655932670004 24.620669923156704 3 19.028302875363227 23.776303161452468 30.539486137146525 26.65590782603778 4 13.068131497111658 17.273476088051158 36.86490415220554 32.79348826263165 5 13.528337292428184 37.28365483567435 34.490819376709716 14.697188495187754 6 32.12342895728959 36.677046383571636 16.78416439675328 14.415360262385493 7 28.146731969209288 33.37477458523059 20.36036425613022 18.1181291894299 8 26.772266112266113 34.31330561330561 19.67975051975052 19.234677754677755 9 26.310018611195655 16.657311172871232 20.179633796866867 36.85303641906625 10-11 24.868805173864068 26.48472293889716 27.12686137402282 21.519610513215948 12-13 26.2245370187496 23.78889327325322 27.474073923154613 22.512495784842564 14-15 22.525614227959874 25.25402170717722 26.069506542797516 26.150857522065387 16-17 21.854844762244728 28.320030170421717 27.351591234028877 22.47353383330468 18-19 21.939795701558122 26.434723167586345 29.99782537377168 21.627655757083854 20-21 22.109420705352285 25.597824708494056 30.54335302416496 21.749401561988698 22-23 22.020522983054963 25.58208673473843 30.523082846606524 21.874307435600088 24-25 22.102629024367477 25.854709961950057 30.316934564531778 21.725726449150688 26-27 21.402943522595038 26.22284948092425 30.12777057359098 22.24643642288974 28-29 21.68986823339612 26.873380983862855 29.774304472746476 21.662446309994554 30-31 21.752228991884028 26.147801402987348 29.672774885437114 22.42719471969151 32-33 21.252185555479326 26.197060700749898 29.80997881492477 22.740774928846005 34-35 22.461454389158092 26.326173419534822 29.349124139667705 21.86324805163938 36-37 22.650408278887486 25.655548798552964 29.469159480854294 22.22488344170526 38-39 21.790985994451344 26.20127616553139 29.368899838514285 22.638838001502986 40-41 22.407213451946742 25.995079884813325 29.507861642085842 22.089845021154083 42-43 22.14287377629851 26.028591222366654 29.210111136338497 22.618423864996338 44-45 21.977504495982203 26.32038421156585 29.544247741613567 22.157863550838382 46-47 22.789209695922388 26.724035773640953 28.47041032244759 22.016344207989068 48-49 22.238006395812743 26.823557147971467 28.309184448636614 22.629252007579176 50-51 22.452454479418183 27.0228077958895 28.372739251504463 22.151998473187856 52-53 23.02097578763673 26.520647930508428 28.337791386401147 22.120584895453703 54-55 22.209711306933734 26.866072546029933 28.550202057287887 22.374014089748446 56-57 22.423556482693844 26.999044910813296 28.206357476119653 22.37104113037321 58-59 22.4010409931597 26.50734237805981 28.193155873299542 22.89846075548095 60-61 21.721418321995202 26.996529329803483 28.621137283779575 22.660915064421744 62-63 21.985616697803046 27.04733990821663 28.764338292007814 22.202705101972505 64-65 21.606242133611865 27.10380534642045 28.47454751766208 22.815405002305603 66-67 21.486492161574315 27.115884293252712 28.478726292727973 22.918897252445 68-69 22.354065302553362 26.65108250272193 28.581244408796568 22.413607785928143 70-71 22.089732645713468 27.047693336953554 27.86912409132432 22.993449926008655 72-73 22.03378695565218 27.04482432720682 28.077397566996577 22.843991150144426 74-75 22.089670275936367 27.542160929825272 27.895485717113143 22.472683077125218 76-77 22.209087609162705 27.02964768144512 28.109725901461573 22.651538807930606 78-79 22.219066773499165 27.444323539476535 27.935319214756188 22.40129047226811 80-81 22.624491114593653 27.445134346578868 27.490290065201368 22.440084473626108 82-83 22.465681542762496 27.58551365991957 27.67522220796957 22.27358258934836 84-85 22.336203482225557 27.853875012864215 27.564053386224742 22.24586811868549 86-87 22.696630101147694 27.81690462944252 27.062849948315964 22.423615321093823 88-89 22.28270247637327 28.015300566442786 26.921271396489576 22.780725560694368 90-91 22.153462850708657 27.959624208759603 27.14694415459757 22.73996878593417 92-93 22.507555602309935 27.425427863202696 27.473515111308572 22.593501423178804 94-95 22.12295494698361 27.17925198678925 27.725735973764777 22.972057092462364 96-97 22.4161968489957 26.74129141197275 27.809186735528446 23.0333250035031 98-99 22.050626795469956 27.240374368350086 27.22869043010614 23.480308406073817 100-101 22.018610309890473 26.92923234030946 27.80008074807142 23.252076601728643 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 708.0 1 503.0 2 440.5 3 585.0 4 474.5 5 443.5 6 913.0 7 2256.5 8 3754.5 9 4244.0 10 3475.5 11 2504.0 12 2307.0 13 2493.5 14 2700.0 15 2857.0 16 3044.5 17 3260.0 18 3567.0 19 4053.0 20 4572.5 21 5384.0 22 6593.0 23 8100.5 24 10173.0 25 12981.5 26 16612.5 27 20700.0 28 23921.5 29 27197.5 30 31429.0 31 37392.5 32 46642.0 33 49168.0 34 46125.5 35 47363.0 36 51012.0 37 54731.5 38 57769.0 39 60714.5 40 64922.0 41 70180.5 42 73100.0 43 74950.5 44 77216.5 45 82949.0 46 86168.5 47 87987.0 48 90179.0 49 87181.5 50 84276.5 51 81897.5 52 80552.5 53 81960.0 54 85658.0 55 81749.5 56 73956.5 57 68065.5 58 63483.5 59 56204.5 60 42854.0 61 35456.5 62 31958.5 63 22794.0 64 14001.5 65 10442.0 66 8002.0 67 6325.0 68 4888.0 69 3799.5 70 2962.5 71 1880.0 72 1229.0 73 951.0 74 699.0 75 489.5 76 341.0 77 221.5 78 128.5 79 61.0 80 32.0 81 21.0 82 12.5 83 4.0 84 2.5 85 2.0 86 0.5 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0156756039785265 3 0.00694383518412182 4 0.0 5 0.0 6 0.0 7 0.0 8 4.573783654211976E-4 9 2.910589598134894E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.7026903411252586E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.006049868378980388 26-27 0.022661019014050248 28-29 4.157985140192706E-5 30-31 0.0 32-33 6.652776224308329E-4 34-35 0.020353337261243293 36-37 0.0040956153630898155 38-39 0.019293051050494156 40-41 0.01632009167525637 42-43 0.009022827754218171 44-45 0.003575867220565727 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.286891827105988E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.078992570096353E-5 84-85 0.00347191759206091 86-87 5.405380682250517E-4 88-89 8.315970280385412E-5 90-91 2.286891827105988E-4 92-93 3.1184888551445293E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2405011.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.563686617265844 #Duplication Level Percentage of deduplicated Percentage of total 1 71.8321940846606 34.884361625580745 2 15.120788105882738 14.6864242996034 3 5.476267376815393 7.978431981600578 4 2.602467624485524 5.055416885883811 5 1.4325525750750296 3.4785017159350464 6 0.8860365840917125 2.581752180075758 7 0.5307687079388214 1.8043259639014413 8 0.38546501491023544 1.4975681748816303 9 0.2962697531427693 1.2949156301220144 >10 1.2906050360001193 11.115231090229381 >50 0.08552863738751251 2.8871066865557227 >100 0.050873288244671945 4.868795991141032 >500 0.0048776726706002424 1.586665463847534 >1k 0.0049632458753464895 4.931836666371749 >5k 2.567196142420598E-4 0.8757828178396311 >10k+ 8.557320474735326E-5 0.47288282643053825 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11379 0.47313712910252803 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9713 0.40386509666691756 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6352 0.2641152161050407 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 5009 0.20827347567225263 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 4899 0.20369969201804067 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 4339 0.18041497523296152 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 4334 0.18020707597595187 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 4315 0.17941705879931524 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 3893 0.16187036150770204 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 3507 0.14582053886655819 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 3242 0.13480187824504752 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 3215 0.1336792222571955 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 2965 0.12328425940671374 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 2786 0.11584146600576879 No Hit GGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCT 2708 0.11259823759641847 No Hit GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA 2470 0.10270223296275985 No Hit CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG 2448 0.10178747623191743 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 4.157985140192706E-5 5 0.0 0.0 0.0 0.0 8.315970280385412E-5 6 0.0 0.0 0.0 0.0 8.315970280385412E-5 7 0.0 0.0 0.0 0.0 1.247395542057812E-4 8 0.0 0.0 0.0 4.157985140192706E-5 1.247395542057812E-4 9 0.0 0.0 0.0 2.078992570096353E-4 1.6631940560770823E-4 10-11 0.0 0.0 0.0 2.078992570096353E-4 2.078992570096353E-4 12-13 0.0 0.0 0.0 2.078992570096353E-4 5.821179196269788E-4 14-15 0.0 0.0 0.0 2.2868918271059884E-4 6.860675481317965E-4 16-17 0.0 0.0 0.0 2.494791084115624E-4 7.0685747383276E-4 18-19 0.0 0.0 0.0 2.494791084115624E-4 8.108071023375776E-4 20-21 0.0 0.0 0.0 2.702690341125259E-4 9.563365822443224E-4 22-23 0.0 0.0 0.0 3.9500858831830707E-4 0.0010394962850481765 24-25 0.0 0.0 0.0 6.236977710289059E-4 0.0010394962850481765 26-27 0.0 0.0 0.0 0.001143445913552994 0.0011226559878520306 28-29 0.0 0.0 0.0 0.003970875808884034 0.0011642358392539576 30-31 0.0 0.0 0.0 0.012058156906558848 0.0011850257649549212 32-33 0.0 0.0 0.0 0.029022736278545087 0.0012473955420578117 34-35 0.0 0.0 0.0 0.053929067268299394 0.0012473955420578117 36-37 0.0 0.0 0.0 0.09180831189545494 0.0012681854677587753 38-39 0.0 0.0 0.0 0.1463818668604842 0.001288975393459739 40-41 0.0 0.0 0.0 0.20754582827271892 0.001288975393459739 42-43 0.0 0.0 0.0 0.268501890427944 0.001288975393459739 44-45 0.0 0.0 0.0 0.3472125491317919 0.0013929250219645566 46-47 0.0 0.0 0.0 0.43521630462397054 0.0015592444275722647 48-49 0.0 0.0 0.0 0.5262761791941908 0.0017879336102828635 50-51 0.0 0.0 0.0 0.6294773703737737 0.0018295134616847907 52-53 2.078992570096353E-5 0.0 0.0 0.7247368099355886 0.0018710933130867177 54-55 4.157985140192706E-5 0.0 0.0 0.8269816645329273 0.0019750429415915354 56-57 4.157985140192706E-5 0.0 0.0 0.9456089805826252 0.002078992570096353 58-59 4.157985140192706E-5 0.0 0.0 1.0673755754131684 0.00212057242149828 60-61 4.157985140192706E-5 2.078992570096353E-5 0.0 1.2040277570456017 0.00212057242149828 62-63 4.157985140192706E-5 4.157985140192706E-5 0.0 1.335544827029897 0.002328471678507915 64-65 4.157985140192706E-5 4.157985140192706E-5 0.0 1.4779973979328993 0.002328471678507915 66-67 4.157985140192706E-5 4.157985140192706E-5 0.0 1.638287725087328 0.002328471678507915 68-69 4.157985140192706E-5 4.157985140192706E-5 0.0 1.8116964953590649 0.002328471678507915 70-71 4.157985140192706E-5 4.157985140192706E-5 0.0 1.9907185455700618 0.0023700515299098423 72-73 4.157985140192706E-5 4.157985140192706E-5 0.0 2.1797821298946243 0.002702690341125259 74-75 4.157985140192706E-5 4.157985140192706E-5 0.0 2.3660390742495565 0.002952169449536821 76-77 4.157985140192706E-5 4.157985140192706E-5 0.0 2.563605738185813 0.0030561190780416386 78-79 4.157985140192706E-5 4.157985140192706E-5 0.0 2.789800129812296 0.0031184888551445294 80-81 4.157985140192706E-5 4.157985140192706E-5 0.0 3.024830239861689 0.00318085863224742 82-83 4.157985140192706E-5 4.157985140192706E-5 0.0 3.2741846087190454 0.003326388112154165 84-85 4.157985140192706E-5 4.157985140192706E-5 0.0 3.531958897485292 0.003388757889257055 86-87 4.157985140192706E-5 4.157985140192706E-5 0.0 3.787425504498732 0.0034927075177618727 88-89 4.157985140192706E-5 4.157985140192706E-5 0.0 4.058671665119203 0.0035966571462666907 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6110 0.0 46.34601 1 GTATCAA 10215 0.0 37.48901 1 TATCAAC 12910 0.0 29.479053 2 ATCAACG 12935 0.0 29.267244 3 TCAACGC 13215 0.0 28.64534 4 CAACGCA 13335 0.0 28.423182 5 TAAGGTG 1975 0.0 28.377737 5 AACGCAG 13690 0.0 27.686129 6 GTAAGGT 2120 0.0 26.884886 4 AAGGTAA 1925 0.0 25.669056 1 AGGTAAG 1950 0.0 25.58362 2 ACGCAGA 15285 0.0 24.703842 7 CGCAGAG 15340 0.0 24.553343 8 GGTAAGG 2265 0.0 23.69737 3 GAGTACT 11070 0.0 22.375332 12-13 ATACCGA 150 3.0366544E-5 22.165094 6 GTGTAAG 1040 0.0 21.928816 1 GCAGAGT 16975 0.0 21.824667 9 TAAGGTA 990 0.0 21.589376 4 GTACTTT 11560 0.0 21.200918 14-15 >>END_MODULE