##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937996_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2413747 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.999744795125586 33.0 31.0 34.0 30.0 34.0 2 32.12108808421098 34.0 31.0 34.0 30.0 34.0 3 32.1423222897843 34.0 31.0 34.0 30.0 34.0 4 35.6607266627364 37.0 35.0 37.0 35.0 37.0 5 35.66684329384977 37.0 35.0 37.0 35.0 37.0 6 35.70151884186702 37.0 36.0 37.0 35.0 37.0 7 35.638870602428504 37.0 35.0 37.0 33.0 37.0 8 35.6280964823571 37.0 35.0 37.0 33.0 37.0 9 37.27441939855337 39.0 37.0 39.0 34.0 39.0 10-11 37.18330462968986 39.0 37.0 39.0 34.0 39.0 12-13 37.036258356820326 39.0 37.0 39.0 33.0 39.0 14-15 38.217935433995365 40.0 38.0 41.0 33.0 41.0 16-17 38.03915219780698 40.0 38.0 41.0 33.0 41.0 18-19 38.22727050515236 40.0 38.0 41.0 33.5 41.0 20-21 38.2211373022939 40.0 38.0 41.0 34.0 41.0 22-23 38.111802313995625 40.0 38.0 41.0 33.0 41.0 24-25 37.981757823002994 40.0 38.0 41.0 33.0 41.0 26-27 37.79081175450451 40.0 38.0 41.0 33.0 41.0 28-29 37.6058153567876 40.0 37.5 41.0 32.0 41.0 30-31 37.35537050900529 40.0 37.0 41.0 31.5 41.0 32-33 37.027601691478026 40.0 36.5 41.0 31.0 41.0 34-35 36.770023950314595 39.5 36.0 41.0 30.5 41.0 36-37 36.47056236631262 39.0 35.5 41.0 30.0 41.0 38-39 36.13960348785518 39.0 35.0 40.5 29.5 41.0 40-41 35.992608794542264 39.0 35.0 40.0 29.0 41.0 42-43 35.74865074922931 39.0 35.0 40.0 27.5 41.0 44-45 35.40015130003269 39.0 35.0 40.0 26.5 41.0 46-47 34.9844552888103 38.0 34.5 40.0 24.5 41.0 48-49 34.533764516330834 38.0 34.0 40.0 23.5 41.0 50-51 33.77739299106327 37.5 33.5 39.5 22.5 40.5 52-53 33.82465042939463 38.0 33.0 39.5 22.0 40.5 54-55 33.87695251407874 38.0 33.5 40.0 21.0 41.0 56-57 33.5362670569865 38.0 33.0 40.0 18.5 41.0 58-59 33.10595745950177 37.0 33.0 40.0 15.5 41.0 60-61 32.582288243134016 36.5 32.0 39.5 10.5 41.0 62-63 32.02565409713611 36.0 31.0 39.0 7.5 41.0 64-65 31.471606593400217 35.0 31.0 39.0 4.0 40.0 66-67 30.87999798653297 35.0 30.0 38.5 2.0 40.0 68-69 30.188224573660786 35.0 29.0 38.0 2.0 40.0 70-71 29.562026177557136 34.0 28.5 37.0 2.0 39.5 72-73 28.86459454947018 34.0 27.5 36.5 2.0 39.0 74-75 28.174143976149946 34.0 26.0 36.0 2.0 39.0 76-77 27.409918272296146 33.0 25.5 35.0 2.0 38.0 78-79 26.620606675016063 32.5 23.5 35.0 2.0 37.0 80-81 25.880598090852107 32.0 21.0 35.0 2.0 36.5 82-83 25.117689219292657 31.5 18.5 35.0 2.0 36.0 84-85 24.378561837673956 31.0 12.5 34.0 2.0 35.5 86-87 23.618041368875858 30.5 4.5 34.0 2.0 35.0 88-89 22.697754984263057 30.0 2.0 34.0 2.0 35.0 90-91 21.762090848792354 29.0 2.0 34.0 2.0 35.0 92-93 20.800001615745146 28.5 2.0 34.0 2.0 35.0 94-95 19.678774743169022 26.5 2.0 33.5 2.0 35.0 96-97 18.388914828273222 24.5 2.0 33.0 2.0 35.0 98-99 16.7896716184422 11.0 2.0 32.0 2.0 34.0 100-101 12.944375487571813 2.0 2.0 28.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1753378556807128 1101 2 0.1326749686824229 1101 3 0.10450575226548864 1101 4 -0.039399053858858224 1101 5 0.14905988654550129 1101 6 0.06541428421787288 1101 7 0.14935134000132422 1101 8 0.24108263640184902 1101 9 0.2508688458703787 1101 10-11 0.2082008557656181 1101 12-13 0.14360294866299483 1101 14-15 0.24432206572818416 1101 16-17 0.29128584043984773 1101 18-19 0.35855169833312317 1101 20-21 0.3261081516808062 1101 22-23 0.29881896767594185 1101 24-25 0.24176523193465727 1101 26-27 0.19940095065300767 1101 28-29 0.2793312632631668 1101 30-31 0.22107851620389596 1101 32-33 0.3008154006079309 1101 34-35 0.2718032700391362 1101 36-37 0.1295809118679685 1101 38-39 0.08751980237558854 1101 40-41 0.08817870138074113 1101 42-43 -0.0016015997710354668 1101 44-45 0.09908043939289968 1101 46-47 0.15651663393677495 1101 48-49 0.09862627395342116 1101 50-51 0.03953709915385417 1101 52-53 0.12313342682283235 1101 54-55 0.019105683674091267 1101 56-57 -0.16566694619415756 1101 58-59 -0.1597877782712942 1101 60-61 -0.16659999149070615 1101 62-63 -0.3404395912520144 1101 64-65 -0.26208837690218445 1101 66-67 -0.41662628864171225 1101 68-69 -0.5484088856161193 1101 70-71 -0.5980573043990773 1101 72-73 -0.629078522358423 1101 74-75 -0.6187787545288863 1101 76-77 -0.5492172075477875 1101 78-79 -0.6506848017925684 1101 80-81 -0.5834297389593424 1101 82-83 -0.6878019027303246 1101 84-85 -0.7179171593814324 1101 86-87 -0.6157820291934009 1101 88-89 -0.6492321665470975 1101 90-91 -0.6986759327406027 1101 92-93 -0.6978202287541713 1101 94-95 -1.1232399630948642 1101 96-97 -1.2458238913942274 1101 98-99 -1.2395714405842533 1101 100-101 -1.466229227932553 1102 1 0.20322838800573706 1102 2 0.20274960292520916 1102 3 0.14156029457470254 1102 4 0.17159250654051306 1102 5 0.20191860046404742 1102 6 0.1212540430158171 1102 7 0.19310473808356932 1102 8 0.26246893039157015 1102 9 0.328269436518859 1102 10-11 0.3010681266730941 1102 12-13 0.2644356641631944 1102 14-15 0.26962310492351804 1102 16-17 0.19043075938014198 1102 18-19 0.2064280513876966 1102 20-21 0.22966671608373446 1102 22-23 0.29956577347863345 1102 24-25 0.21460914874960935 1102 26-27 0.2067516203841251 1102 28-29 0.18118229133749963 1102 30-31 0.15910599124344316 1102 32-33 0.10376833407966046 1102 34-35 0.041740745325810735 1102 36-37 0.16000456004906738 1102 38-39 0.024849125971869057 1102 40-41 0.06856327161601428 1102 42-43 0.16302698983948716 1102 44-45 0.1925230674079259 1102 46-47 0.2135387708980261 1102 48-49 0.07612401886247966 1102 50-51 -0.0903175247093273 1102 52-53 -0.10349857940492768 1102 54-55 -0.03952281943978875 1102 56-57 -0.077654299149863 1102 58-59 -0.04463681074460624 1102 60-61 -0.09612531029260651 1102 62-63 -0.3591939160839601 1102 64-65 -0.3006164512398648 1102 66-67 -0.3342274279260131 1102 68-69 -0.3700535883148106 1102 70-71 -0.6347882566035068 1102 72-73 -0.6206667242168677 1102 74-75 -0.5845577494379377 1102 76-77 -0.678241618134976 1102 78-79 -0.5758906946850146 1102 80-81 -0.7478969196631766 1102 82-83 -0.7276853485092865 1102 84-85 -0.7064679390868491 1102 86-87 -0.5978579176374481 1102 88-89 -0.5844886599733528 1102 90-91 -0.7019373194052889 1102 92-93 -0.7393355263026109 1102 94-95 -0.9980484348228238 1102 96-97 -1.1854237517639383 1102 98-99 -1.286291982300348 1102 100-101 -1.1433442252140864 1103 1 0.18091472661242491 1103 2 0.26290140614900537 1103 3 0.2542046092779131 1103 4 0.1893102939442528 1103 5 0.20220072786156607 1103 6 0.2276822130067231 1103 7 0.30252140325551835 1103 8 0.20825525494793595 1103 9 0.29489152331992585 1103 10-11 0.21034912780309867 1103 12-13 0.2862939980944148 1103 14-15 0.3439355548505816 1103 16-17 0.3544487916181467 1103 18-19 0.332354307396443 1103 20-21 0.30682827904708887 1103 22-23 0.40636192354200773 1103 24-25 0.38045157368503624 1103 26-27 0.2997383816961232 1103 28-29 0.35295340866662883 1103 30-31 0.3580830645621873 1103 32-33 0.3304012725093699 1103 34-35 0.05673497572711028 1103 36-37 0.08097693921633464 1103 38-39 0.04163168907872006 1103 40-41 0.17988934138331558 1103 42-43 0.32304264323906295 1103 44-45 0.44082899514743445 1103 46-47 0.4643615179145755 1103 48-49 0.52988903700831 1103 50-51 0.5348642699647002 1103 52-53 0.44526144265705625 1103 54-55 0.41927322150941393 1103 56-57 0.4068999731931271 1103 58-59 0.5060544761150538 1103 60-61 0.42323554223286664 1103 62-63 0.44862052704512934 1103 64-65 0.2172782730151681 1103 66-67 0.06492099574787602 1103 68-69 -0.028381708830668373 1103 70-71 -0.22863513279927972 1103 72-73 -0.020563065753197662 1103 74-75 -0.0815740186599676 1103 76-77 0.12484252010222008 1103 78-79 -0.013893385918809287 1103 80-81 -0.12103450516085701 1103 82-83 -0.30984897417891943 1103 84-85 -0.42500447514534656 1103 86-87 -0.6485499518544557 1103 88-89 -0.5111089120121441 1103 90-91 -0.3683808064849785 1103 92-93 -0.5465483467694376 1103 94-95 -0.7290483690553771 1103 96-97 -0.9823857254748951 1103 98-99 -0.9275081419597928 1103 100-101 -1.1257018274824642 1104 1 0.32167517213974506 1104 2 0.26058339278333165 1104 3 0.36181046138184314 1104 4 0.3091694353577594 1104 5 0.31049138983053126 1104 6 0.2762222618667778 1104 7 0.3732690650606685 1104 8 0.3928890648907952 1104 9 0.3818315370596608 1104 10-11 0.39270038936799523 1104 12-13 0.5129464715097285 1104 14-15 0.4027727857951291 1104 16-17 0.3989045618804923 1104 18-19 0.48612822653518606 1104 20-21 0.4733288679835752 1104 22-23 0.6212113587960957 1104 24-25 0.5780262039013166 1104 26-27 0.5055421589077937 1104 28-29 0.47200175334058514 1104 30-31 0.5375285554202875 1104 32-33 0.6093755778990229 1104 34-35 0.6696796236795066 1104 36-37 0.5115116405730191 1104 38-39 0.3782220869741124 1104 40-41 0.5592728274700676 1104 42-43 0.7203035670448514 1104 44-45 0.5541175899883442 1104 46-47 0.4567158652055099 1104 48-49 0.3385640451126548 1104 50-51 0.34459751339293376 1104 52-53 0.4663019763262142 1104 54-55 0.4672649689609685 1104 56-57 0.4971178225491073 1104 58-59 0.5814339923000631 1104 60-61 0.5437441192269858 1104 62-63 0.3399134462562543 1104 64-65 0.1935014023912629 1104 66-67 0.372413142203218 1104 68-69 0.25495052699281473 1104 70-71 0.2019621538298395 1104 72-73 0.35075109254110615 1104 74-75 0.34829990920442455 1104 76-77 0.09844450570615493 1104 78-79 0.11779845363286867 1104 80-81 0.014648732816102239 1104 82-83 0.10464412411678481 1104 84-85 0.041063150690508365 1104 86-87 -0.06838765100940947 1104 88-89 -0.009282510496600338 1104 90-91 -0.2203955715298882 1104 92-93 -0.577786107493786 1104 94-95 -0.7743095845629533 1104 96-97 -0.8665101820034415 1104 98-99 -0.8231344110122443 1104 100-101 -1.032218024641674 1105 1 0.20728782428024672 1105 2 0.1864758986077959 1105 3 0.21826202867210043 1105 4 0.16632555967960627 1105 5 0.21325798069970858 1105 6 0.19114438178042548 1105 7 0.2367588708047279 1105 8 0.20444729157171793 1105 9 0.3366403831956504 1105 10-11 0.2347897116979496 1105 12-13 0.20969925962485547 1105 14-15 0.1897722421899246 1105 16-17 0.29959719590780054 1105 18-19 0.34310742604248645 1105 20-21 0.16683355391628396 1105 22-23 0.2283936683396277 1105 24-25 0.14327558671563878 1105 26-27 -0.020651965756904644 1105 28-29 0.09638469859148557 1105 30-31 0.03251137966054074 1105 32-33 0.09189711698953573 1105 34-35 0.02363312082061242 1105 36-37 0.08235601970569917 1105 38-39 0.03729730873676118 1105 40-41 -0.023833043570860468 1105 42-43 0.11126315417024557 1105 44-45 0.08765483637137805 1105 46-47 0.20246818582714354 1105 48-49 0.15403739885648804 1105 50-51 -0.041409750038432946 1105 52-53 -0.16646137455819598 1105 54-55 -0.1291610625010975 1105 56-57 -0.12044954591641499 1105 58-59 0.004411384677929675 1105 60-61 -0.015612977718731713 1105 62-63 -0.07127204252233099 1105 64-65 -0.1624064724126555 1105 66-67 -0.2871686763150443 1105 68-69 -0.441077541470019 1105 70-71 -0.7386602374674496 1105 72-73 -0.7010751346467679 1105 74-75 -0.7990863168801745 1105 76-77 -0.9054886018756534 1105 78-79 -0.9414954496445098 1105 80-81 -0.9807458103931772 1105 82-83 -1.0834994868113235 1105 84-85 -0.822286651401587 1105 86-87 -0.6961515448007525 1105 88-89 -0.6601279789345078 1105 90-91 -0.6985972473142468 1105 92-93 -0.7734586679301501 1105 94-95 -0.8497885733665029 1105 96-97 -0.9409440313252944 1105 98-99 -1.078686463567033 1105 100-101 -1.078521439438978 1106 1 0.29063772628837015 1106 2 0.305046869409459 1106 3 0.2783681135295808 1106 4 0.3112679266944909 1106 5 0.4087928795294431 1106 6 0.35416453982358576 1106 7 0.3949712461446211 1106 8 0.23529263002674128 1106 9 0.5240582982469562 1106 10-11 0.4721518799927438 1106 12-13 0.4750504201855108 1106 14-15 0.5515798932772427 1106 16-17 0.09724577091508735 1106 18-19 0.46567938176351475 1106 20-21 0.4830939846804867 1106 22-23 0.5999195972822164 1106 24-25 0.5711822490031082 1106 26-27 0.6354811575776509 1106 28-29 0.4533451621996747 1106 30-31 0.46880555851502237 1106 32-33 0.5489863162004767 1106 34-35 0.5135631003341317 1106 36-37 0.6021355169807734 1106 38-39 0.5200024125953462 1106 40-41 0.5007410348074401 1106 42-43 0.3991851503161712 1106 44-45 0.5234255517820898 1106 46-47 0.3685727176964093 1106 48-49 0.2769829998633284 1106 50-51 0.262848426052507 1106 52-53 0.28635120632660005 1106 54-55 0.2692020191043909 1106 56-57 0.09715672796397712 1106 58-59 0.18563294873149516 1106 60-61 0.3429395788511229 1106 62-63 0.3652250609266048 1106 64-65 0.17841273487526976 1106 66-67 0.05141956057926933 1106 68-69 0.07934843415822712 1106 70-71 0.1092069240325273 1106 72-73 0.1736099771887858 1106 74-75 0.2213338580450248 1106 76-77 -0.2265152103005974 1106 78-79 0.05769335203825321 1106 80-81 0.02755039510692825 1106 82-83 0.19268878720749072 1106 84-85 0.06780502770991959 1106 86-87 -0.055403986196651545 1106 88-89 -0.5843602958913188 1106 90-91 -0.7565756742995973 1106 92-93 -0.7156448608967381 1106 94-95 -0.9400090080831909 1106 96-97 -0.7713003705136039 1106 98-99 -0.6336701275871128 1106 100-101 -1.1338422443205296 1107 1 -0.13126704105131992 1107 2 -0.05353163780847581 1107 3 -0.03550483705998175 1107 4 0.09344847749192553 1107 5 -0.10971599150020239 1107 6 0.03443524986123947 1107 7 -0.01150634787610727 1107 8 -0.060209790432764976 1107 9 -0.09708258433300898 1107 10-11 -0.03562217903601805 1107 12-13 -0.09034423427794991 1107 14-15 -0.021209510270068677 1107 16-17 0.07727046794116177 1107 18-19 0.05459336980489837 1107 20-21 0.12083338208852012 1107 22-23 0.16962012375079638 1107 24-25 0.06499888021167521 1107 26-27 -0.06483112652357192 1107 28-29 -0.06831600365974566 1107 30-31 -0.13526619245737237 1107 32-33 -0.12974506369430827 1107 34-35 -0.08466574956977269 1107 36-37 -0.12071841804005601 1107 38-39 -0.07965154186499745 1107 40-41 -0.11913388587434781 1107 42-43 -0.17707133573661338 1107 44-45 -0.4024790439891248 1107 46-47 -0.48011381692020905 1107 48-49 -0.33137106006009276 1107 50-51 -0.4590427373051753 1107 52-53 -0.4864674485433227 1107 54-55 -0.3964696301050594 1107 56-57 -0.48958430468318426 1107 58-59 -0.5081454661487257 1107 60-61 -0.43387421024812767 1107 62-63 -0.5934196621550427 1107 64-65 -0.5923042980649633 1107 66-67 -0.5469458814037971 1107 68-69 -0.6987650966862731 1107 70-71 -0.8602233268422097 1107 72-73 -0.9663232694153265 1107 74-75 -0.9315378063331359 1107 76-77 -1.1065165835848418 1107 78-79 -1.3129675713794278 1107 80-81 -1.2310772263518714 1107 82-83 -1.190881100166635 1107 84-85 -1.2649317124114994 1107 86-87 -1.122682304339488 1107 88-89 -1.1870508469041354 1107 90-91 -1.2088254213189202 1107 92-93 -1.5312083685739708 1107 94-95 -1.531346953270738 1107 96-97 -1.562699874179959 1107 98-99 -1.7917577869345678 1107 100-101 -1.6512365644831597 1108 1 0.02128314718005342 1108 2 0.05943283432173985 1108 3 0.035490276735764326 1108 4 0.12337560414499649 1108 5 0.05192019322653607 1108 6 -0.009830309685511907 1108 7 0.14040420928642305 1108 8 -0.02275501201413732 1108 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-0.4269318007964529 2316 68-69 -0.3181129290340863 2316 70-71 -0.4256068323431421 2316 72-73 -0.4081800988948352 2316 74-75 -0.34952553983725565 2316 76-77 -0.49204613617910553 2316 78-79 -0.4017418371162442 2316 80-81 -0.3454048146187141 2316 82-83 -0.4535338289900501 2316 84-85 -0.607372125750608 2316 86-87 -0.6889162636962034 2316 88-89 -0.6460733710283684 2316 90-91 -0.38736505589037407 2316 92-93 -0.5464447519736808 2316 94-95 -0.6024111768655409 2316 96-97 -0.6630895003823234 2316 98-99 -0.8213803850261847 2316 100-101 -0.5827152193032585 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7678.0 3 2220.0 4 1987.0 5 2359.0 6 2758.0 7 3401.0 8 4477.0 9 5889.0 10 7310.0 11 9407.0 12 12371.0 13 15082.0 14 18344.0 15 22578.0 16 26235.0 17 32876.0 18 32348.0 19 28018.0 20 27829.0 21 28637.0 22 31537.0 23 34837.0 24 39408.0 25 43764.0 26 49426.0 27 57138.0 28 65386.0 29 75193.0 30 88813.0 31 106151.0 32 127823.0 33 158320.0 34 201994.0 35 259931.0 36 315667.0 37 314209.0 38 144591.0 39 7755.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.37977915932847 18.477307096316487 15.02886528221181 23.11404846214323 2 15.303778752254438 19.819620031478202 40.25777804675139 24.61882316951597 3 18.971422928296118 23.855346598290712 30.331297517976076 26.8419329554371 4 12.773225262195442 16.902059355879313 37.14851332352352 33.17620205840173 5 13.514464913799152 37.220144568190435 34.42504608821956 14.84034442979086 6 31.87611332906302 36.5691261370953 17.048469433292414 14.506291100549259 7 28.026112880816306 33.44543618022552 20.49801385405272 18.030437084905458 8 26.747698438428923 34.11675838640696 19.767061986071646 19.368481189092467 9 26.378436810636412 16.74532860891561 20.307100979036292 36.569133601411686 10-11 24.830823910469487 26.386700646346593 27.191886349168172 21.590589094015748 12-13 26.30363522445779 23.84414301896657 27.429409592411712 22.422812164163933 14-15 22.59112340151715 25.24469775717146 26.136614210713994 26.0275646305974 16-17 21.889395233967313 28.27230952059431 27.313095710006362 22.52519953543202 18-19 22.005919677170372 26.40952226267826 29.966244688978406 21.618313371172967 20-21 22.169782553770368 25.569607229372522 30.5535745475108 21.70703566934632 22-23 22.013775604563804 25.538317772140633 30.55136667339886 21.896539949896702 24-25 22.06009512130156 25.766064046294662 30.397585781588887 21.77625505081489 26-27 21.39995908796875 26.12531944371256 30.18444853195931 22.290272936359383 28-29 21.667045210589688 26.827376711355594 29.844776249428296 21.660801828626425 30-31 21.67433841774333 26.079191216753962 29.71939182830982 22.527078537192892 32-33 21.254610372451303 26.135787109998066 29.83389591872885 22.775706598821785 34-35 22.433800118398285 26.33331294366958 29.378222387643447 21.854664550288685 36-37 22.66057254322874 25.570064484666933 29.47779258873147 22.291570383372864 38-39 21.7905680231017 26.18847912103126 29.327262360653332 22.69369049521371 40-41 22.43602924045942 26.002477330518836 29.492130661773913 22.06936276724783 42-43 22.131662169755845 25.99753379834231 29.19258945577533 22.678214576126514 44-45 21.979550205650114 26.32385707109808 29.539554283838605 22.157038439413203 46-47 22.792278458949756 26.725192581540814 28.42644264915601 22.056086310353425 48-49 22.3060017676943 26.848996838666295 28.210204341730744 22.634797051908663 50-51 22.507781414884857 27.081769416438444 28.263271928999174 22.147177239677525 52-53 22.988393810640133 26.594633727693196 28.251479730090935 22.16549273157574 54-55 22.210565497162317 26.879664186837733 28.54621202951919 22.36355828648076 56-57 22.40318691617251 27.062203586109497 28.139296217153216 22.39531328056478 58-59 22.46602324860354 26.534280167061 28.089389073388453 22.910307510947007 60-61 21.76740774050362 26.91508199405055 28.6409409707076 22.67656929473823 62-63 22.081303279836916 26.917245112742062 28.756500523523748 22.24495108389727 64-65 21.71662433644897 27.031062974764335 28.480285778704694 22.772026910081998 66-67 21.588499153069566 26.968755789361126 28.50253498898182 22.94021006858748 68-69 22.451109154316473 26.52918111461012 28.567398308865112 22.452311422208293 70-71 22.1894659261404 26.894082042224255 27.890443177901435 23.026008853733902 72-73 22.098396562487306 26.964896979906666 28.06392858356802 22.872777874038007 74-75 22.263025587993308 27.382510139244943 27.894124937050673 22.460339335711073 76-77 22.483094447942587 26.893860047700418 27.92966892529856 22.693376579058437 78-79 22.561715426556837 27.326236691206145 27.670792216650213 22.441255665586795 80-81 22.963011922427764 27.299400832841936 27.28139556759682 22.45619167713348 82-83 22.793644005727547 27.395965481397592 27.471403184792955 22.33898732808191 84-85 22.682154433602918 27.698019622488268 27.37072952934827 22.249096414560547 86-87 23.123737000676915 27.579626584207517 26.87772374292653 22.418912672189037 88-89 22.77062343172992 27.73262219421519 26.70285239890261 22.79390197515228 90-91 22.633987231726902 27.70467229868734 26.86497086657022 22.796369603015542 92-93 23.051915338255142 27.0847716251713 27.143796012381234 22.719517024192328 94-95 22.6944101459719 26.865219579788658 27.397470140979852 23.042900133259586 96-97 23.076843348159056 26.413170018208774 27.399001834144272 23.1109847994879 98-99 22.708611979738276 26.883183343582758 26.780005152691494 23.62819952398748 100-101 23.608147405536357 25.532777287197643 27.23493478188268 23.62414052538332 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 690.0 1 481.0 2 423.5 3 593.0 4 504.0 5 457.5 6 860.5 7 2042.0 8 3398.0 9 3920.5 10 3256.0 11 2406.5 12 2246.5 13 2420.0 14 2658.5 15 2824.5 16 3092.5 17 3419.0 18 3745.5 19 4140.0 20 4668.5 21 5631.0 22 6972.0 23 8538.5 24 10665.0 25 13673.5 26 17331.0 27 21361.0 28 24735.5 29 27867.0 30 31761.0 31 37956.0 32 46431.5 33 48506.0 34 46338.5 35 47631.5 36 51000.0 37 54770.5 38 58079.5 39 60834.5 40 64481.0 41 69402.5 42 72147.0 43 73501.5 44 75573.0 45 80933.0 46 84500.5 47 86696.0 48 88407.5 49 85898.5 50 83250.0 51 81027.5 52 79991.0 53 80927.0 54 83852.0 55 81228.5 56 74416.0 57 68823.5 58 64543.5 59 57194.0 60 44876.5 61 37127.5 62 32898.0 63 24947.0 64 16887.0 65 12650.0 66 9619.0 67 7477.5 68 5815.5 69 4559.5 70 3635.5 71 2530.0 72 1698.0 73 1248.5 74 934.5 75 677.5 76 483.5 77 337.5 78 209.5 79 110.0 80 72.5 81 57.0 82 36.0 83 17.5 84 13.5 85 11.0 86 8.0 87 9.0 88 7.5 89 5.5 90 2.5 91 1.5 92 1.5 93 0.5 94 0.0 95 1.0 96 1.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1413569856327113 2 0.053195301744549034 3 0.15486295788249554 4 0.13406541779233697 5 0.19803235384652987 6 0.20167813776671706 7 0.1679960658677152 8 0.06997419364995586 9 0.12466095245276328 10-11 0.10365626554895771 12-13 0.15399294126517818 14-15 0.13984481389308823 16-17 0.13818763938391224 18-19 0.13719333467840664 20-21 0.13911980004532373 22-23 0.20397746739819872 24-25 0.22158494655819355 26-27 0.15320578337331955 28-29 0.1323460992390669 30-31 0.1044019940780869 32-33 0.1305024925976086 34-35 0.09920260905554724 36-37 0.09294677528340792 38-39 0.053174587063184335 40-41 0.061025451300405556 42-43 0.06361478647099303 44-45 0.037742149446483 46-47 0.049611661868455975 48-49 0.04140864804803486 50-51 0.052532431940878636 52-53 0.02643193342135692 54-55 0.05033667571622047 56-57 0.026121213200886423 58-59 0.05033667571622047 60-61 0.0380942990296829 62-63 0.051745274049020046 64-65 0.04022791121024697 66-67 0.03794929626013 68-69 0.06790272551348588 70-71 0.054624614758713316 72-73 0.05406531836186642 74-75 0.04524086410050432 76-77 0.023221157809828454 78-79 0.020424675825593982 80-81 0.023366160579381352 82-83 0.020611107957876282 84-85 0.018415351733218104 86-87 0.02454689741716924 88-89 0.06854488063579157 90-91 0.11480076412316619 92-93 0.0841844650661399 94-95 0.07925437090134135 96-97 0.07138279198275543 98-99 0.05864326294346508 100-101 0.13818763938391224 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2413747.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.98156073673903 #Duplication Level Percentage of deduplicated Percentage of total 1 74.04097298941815 37.747243614672755 2 14.105677437566685 14.382589020323106 3 5.1072238211432195 7.811227243012003 4 2.3532000411804654 4.798792433005547 5 1.2735748683267885 3.2464417251193285 6 0.7446751794176842 2.277882173317482 7 0.4921545061230349 1.7563563392039916 8 0.33147613089158634 1.3519336399862933 9 0.25113859034770597 1.1523093567435534 >10 1.166098071972896 10.518743728173021 >50 0.07864577716325961 2.800027104165345 >100 0.046111991952378646 4.63358563792218 >500 0.004239918142390155 1.492734808968344 >1k 0.004566065691803815 4.911933053937826 >5k 1.630737747072791E-4 0.6455631184426258 >10k+ 8.153688735363956E-5 0.4726370030066101 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11370 0.471051854233273 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9601 0.3977633115649652 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5929 0.24563469162261 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 4974 0.2060696502160334 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 4817 0.19956524026751768 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 4438 0.18386351179307525 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 4294 0.1778976835600417 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 4134 0.1712689855233378 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 3767 0.15606440940164815 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 3634 0.150554304158638 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 3193 0.1322839551949728 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 3165 0.1311239330385496 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 3073 0.12731243166744485 No Hit GGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCT 2632 0.10904208270377964 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 2561 0.10610059794999227 No Hit GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA 2550 0.10564487495996887 No Hit CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG 2459 0.10187480295159351 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.2428808818819867E-4 2 0.0 0.0 0.0 0.0 1.6571745091759825E-4 3 0.0 0.0 0.0 0.0 1.6571745091759825E-4 4 0.0 0.0 0.0 8.285872545879912E-5 1.6571745091759825E-4 5 0.0 0.0 0.0 8.285872545879912E-5 1.6571745091759825E-4 6 0.0 0.0 0.0 8.285872545879912E-5 2.4857617637639735E-4 7 0.0 0.0 0.0 8.285872545879912E-5 2.4857617637639735E-4 8 0.0 0.0 0.0 1.6571745091759825E-4 2.4857617637639735E-4 9 0.0 0.0 0.0 2.900055391057969E-4 2.4857617637639735E-4 10-11 0.0 0.0 0.0 3.107202204704967E-4 2.4857617637639735E-4 12-13 0.0 0.0 0.0 3.5214958319989626E-4 6.62869803670393E-4 14-15 0.0 0.0 0.0 3.72864264564596E-4 8.907312986820905E-4 16-17 0.0 0.0 0.0 4.3500830865869536E-4 0.0010771634309643886 18-19 0.0 0.0 0.0 4.7643767138809493E-4 0.0010978781123290884 20-21 0.0 0.0 0.0 5.59296396846894E-4 0.0012014515191525873 22-23 0.0 0.0 0.0 8.078725732232914E-4 0.0013464542887054857 24-25 0.0 0.0 0.0 0.0011807368377878874 0.0013671689700701854 26-27 0.0 0.0 0.0 0.00188503600418768 0.0014707423768936844 28-29 0.0 0.0 0.0 0.004453656493410453 0.001491457058258384 30-31 0.0 0.0 0.0 0.012180232642443469 0.0015121717396230838 32-33 0.0 0.0 0.0 0.02939413285650899 0.0015743157837171833 34-35 0.0 0.0 0.0 0.05427246517551343 0.0016157451464465828 36-37 0.0 0.0 0.0 0.09358893040571362 0.0016157451464465828 38-39 0.0 0.0 0.0 0.15196290249143757 0.0016157451464465828 40-41 0.0 0.0 0.0 0.21452124021283092 0.0016157451464465828 42-43 0.0 0.0 0.0 0.2787160377620356 0.0016157451464465828 44-45 0.0 0.0 0.0 0.3552360707232365 0.0016571745091759824 46-47 0.0 0.0 0.0 0.44633923936518616 0.0017400332346347814 48-49 0.0 0.0 0.0 0.5406946129813937 0.0019057506855523797 50-51 0.0 0.0 0.0 0.6450551776967511 0.0019886094110111788 52-53 0.0 0.0 0.0 0.7429941911890516 0.002175041543293477 54-55 0.0 0.0 0.0 0.8474583293112327 0.002278614950116976 56-57 0.0 0.0 0.0 0.9661741682123272 0.0023407589942110755 58-59 0.0 0.0 0.0 1.0913115583364785 0.0024029030383051746 60-61 0.0 0.0 0.0 1.2249626825015216 0.0024029030383051746 62-63 0.0 0.0 0.0 1.3573087817405884 0.0027757673028697707 64-65 0.0 0.0 0.0 1.492927800635278 0.0027757673028697707 66-67 0.0 0.0 0.0 1.6511465368988547 0.0027757673028697707 68-69 0.0 0.0 0.0 1.8243005584263803 0.0027964819842344704 70-71 0.0 0.0 0.0 2.002902541152822 0.0028379113469638698 72-73 0.0 0.0 0.0 2.1875324961563907 0.002982914116516768 74-75 0.0 0.0 0.0 2.373757481625042 0.003086487523340267 76-77 0.0 0.0 0.0 2.5743791706421595 0.0031486315674343667 78-79 0.0 0.0 0.0 2.803172826315268 0.0031486315674343667 80-81 0.0 0.0 0.0 3.0383051744859753 0.0031693462487990664 82-83 0.0 0.0 0.0 3.283691289932209 0.003355778381081364 84-85 0.0 0.0 0.0 3.539103311158957 0.003355778381081364 86-87 0.0 0.0 0.0 3.7974361024581285 0.003397207743810764 88-89 0.0 0.0 0.0 4.060781846647557 0.0034386371065401634 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6185 0.0 46.811523 1 GTATCAA 10475 0.0 39.459774 1 TATCAAC 13210 0.0 31.105713 2 ATCAACG 13220 0.0 31.046207 3 TCAACGC 13530 0.0 30.37003 4 CAACGCA 13595 0.0 30.154856 5 AACGCAG 13945 0.0 29.530136 6 TAAGGTG 2135 0.0 28.512896 5 GTGTAAG 1060 0.0 26.954687 1 CGCAGAG 15465 0.0 26.343882 8 ACGCAGA 15550 0.0 26.322712 7 AGGTAAG 2070 0.0 26.191704 2 AAGGTAA 2045 0.0 25.148918 1 GGTAAGG 2300 0.0 24.81319 3 TAAGGTA 1005 0.0 24.607445 4 GCAGAGT 16785 0.0 23.761862 9 TGGTATC 3290 0.0 23.707033 2 GTGGTAT 3410 0.0 23.600502 1 GTAAGGT 2345 0.0 23.525797 4 GAGTACT 11335 0.0 22.353964 12-13 >>END_MODULE