FastQCFastQC Report
Thu 26 May 2016
SRR937996_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937996_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2413747
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112090.46438172683383966No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93100.3857073670107099No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60290.24977762789554994No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC51420.21302978315457252No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA48460.20076669178667028No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA44190.18307635390121665No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA44050.18249634282300506No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA41690.17271901321886676No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG38320.15875731797905912No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA34600.14334559504372246No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA33560.1390369413198649No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA31790.1317039441167612No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT30660.12702242612833906No Hit
GGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCT28110.11645793863234216No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA27500.11393074750584879No Hit
GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA24920.1032419719216637No Hit
TCAGTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAG24620.10199909103978172No Hit
ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT24300.10067335143244094No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59950.051.0277941
GTATCAA102250.039.534511
TATCAAC128550.031.481152
ATCAACG128250.031.4755023
TCAACGC130950.030.8252454
CAACGCA132650.030.43025
AACGCAG136350.029.5690026
TAAGGTG21100.027.6831675
ACGCAGA151900.026.4164267
AAGGTAA18500.026.1901841
CGCAGAG152600.026.077428
AGGTAAG20000.025.649382
GTAAGGT24200.024.5294484
TAAGGTA10500.023.9706444
GGTAAGG23300.023.84743
GCAGAGT165750.023.7506839
GTGTAAG11500.023.544371
GAGTACT112250.023.37625912-13
GTACTTT116400.022.3184214-15
GTACATG70350.021.8771631
Produced by FastQC (version 0.11.5)