##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937996_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2413747 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.52424425592243 34.0 31.0 34.0 31.0 34.0 2 32.74268098520682 34.0 31.0 34.0 31.0 34.0 3 32.792772813389305 34.0 31.0 34.0 31.0 34.0 4 36.252054999964784 37.0 35.0 37.0 35.0 37.0 5 36.22355035552608 37.0 37.0 37.0 35.0 37.0 6 36.25728255695398 37.0 37.0 37.0 35.0 37.0 7 36.21887173759305 37.0 37.0 37.0 35.0 37.0 8 36.20342397111214 37.0 37.0 37.0 35.0 37.0 9 37.896902616554264 39.0 38.0 39.0 35.0 39.0 10-11 37.87048476911623 39.0 38.0 39.0 35.0 39.0 12-13 37.795012277591645 39.0 38.0 39.0 35.0 39.0 14-15 39.0703847586346 40.0 38.5 41.0 36.0 41.0 16-17 38.954879902491854 40.0 38.0 41.0 36.0 41.0 18-19 39.21564749743863 40.5 39.0 41.0 36.0 41.0 20-21 39.197111793406684 40.5 39.0 41.0 36.0 41.0 22-23 39.106093347811516 40.0 39.0 41.0 35.5 41.0 24-25 38.99545312744045 40.0 38.5 41.0 35.0 41.0 26-27 38.817427220002756 40.0 38.0 41.0 35.0 41.0 28-29 38.697866222101986 40.0 38.0 41.0 34.5 41.0 30-31 38.43130721653927 40.0 38.0 41.0 34.0 41.0 32-33 38.19303390123323 40.0 38.0 41.0 34.0 41.0 34-35 38.01490649185685 40.0 37.5 41.0 33.0 41.0 36-37 37.97057583085551 40.0 38.0 41.0 33.0 41.0 38-39 37.802342581886165 40.0 37.0 41.0 33.0 41.0 40-41 37.613218991054154 40.0 37.0 41.0 33.0 41.0 42-43 37.371957168667635 40.0 37.0 41.0 32.5 41.0 44-45 37.08442330534227 40.0 36.0 41.0 31.5 41.0 46-47 36.67257421759612 39.5 36.0 41.0 30.5 41.0 48-49 36.23821448561096 39.0 35.0 41.0 30.0 41.0 50-51 35.83400290088397 39.0 35.0 40.5 28.5 41.0 52-53 35.298565881179755 38.0 35.0 40.0 27.0 41.0 54-55 34.95873801189603 38.0 34.0 40.0 26.0 41.0 56-57 34.59380519167916 38.0 34.0 40.0 26.0 41.0 58-59 34.13080782700092 37.5 33.5 40.0 24.5 41.0 60-61 33.63416836975872 37.0 33.0 40.0 23.0 41.0 62-63 33.16594137662315 36.0 32.5 39.0 22.0 40.5 64-65 32.66601118509935 36.0 32.0 39.0 20.5 40.0 66-67 32.103469833416675 35.0 31.0 38.5 18.0 40.0 68-69 31.50733237576266 35.0 31.0 38.0 14.0 40.0 70-71 30.867621793004815 34.5 30.0 37.0 8.0 39.5 72-73 30.280236081080577 34.0 29.0 36.5 7.0 39.0 74-75 29.68165201241058 34.0 29.0 36.0 4.0 39.0 76-77 28.335520872734385 32.5 27.0 34.5 2.0 36.5 78-79 29.004532786576224 34.0 29.0 35.0 2.0 37.0 80-81 28.677658843283908 34.0 28.5 35.0 2.0 37.0 82-83 28.101301420571417 33.0 27.0 35.0 2.0 36.0 84-85 27.49211847803436 33.0 26.0 35.0 2.0 36.0 86-87 26.844217103118098 32.5 25.0 35.0 2.0 35.5 88-89 26.06528107543997 32.0 23.5 34.5 2.0 35.0 90-91 25.49638922389132 32.0 21.5 34.0 2.0 35.0 92-93 24.671298814664503 31.0 18.5 34.0 2.0 35.0 94-95 23.81617481036745 31.0 6.5 34.0 2.0 35.0 96-97 22.733344878315748 30.5 2.0 34.0 2.0 35.0 98-99 21.231139179044035 29.5 2.0 34.0 2.0 35.0 100-101 16.86522717583906 21.5 2.0 30.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.08866975036885805 1101 2 0.11912813582308246 1101 3 0.10978511033460592 1101 4 0.046057839556084446 1101 5 0.10544959354428585 1101 6 0.03712360478149179 1101 7 0.044331250419958224 1101 8 0.027902310123359086 1101 9 -0.10276334870133752 1101 10-11 0.06492936357283696 1101 12-13 0.07100552554098272 1101 14-15 0.11233390740954263 1101 16-17 0.22720191637186105 1101 18-19 0.18828087658397408 1101 20-21 0.19890166775702767 1101 22-23 0.24977535523935757 1101 24-25 0.2417916857597575 1101 26-27 0.20990694534889798 1101 28-29 0.1843222457671274 1101 30-31 0.22831863428459087 1101 32-33 0.23885474308249144 1101 34-35 0.2269705028646385 1101 36-37 0.2753590227899707 1101 38-39 0.23445810358710162 1101 40-41 0.15983221239705614 1101 42-43 0.21379318924090285 1101 44-45 0.19698064546775385 1101 46-47 0.23101706256941412 1101 48-49 0.1372649422945429 1101 50-51 -0.012858398774618252 1101 52-53 -0.21261175625254225 1101 54-55 -0.16861420349445666 1101 56-57 0.04231705524426843 1101 58-59 0.12123152441821361 1101 60-61 0.01872168782593775 1101 62-63 0.025962800979463907 1101 64-65 -0.04486973914171699 1101 66-67 -0.274078979242784 1101 68-69 -0.18694575995727192 1101 70-71 -0.25308175312952486 1101 72-73 -0.22509601321849715 1101 74-75 -0.2992331386146496 1101 76-77 -0.3595386804555112 1101 78-79 -0.2986275493075574 1101 80-81 -0.2312571052213741 1101 82-83 -0.33705333083541333 1101 84-85 -0.4423995037999191 1101 86-87 -0.4408881289915314 1101 88-89 -0.5428735220757375 1101 90-91 -0.5519007139305856 1101 92-93 -0.7264068755449848 1101 94-95 -0.7010682687393057 1101 96-97 -0.667696612033069 1101 98-99 -0.6707060563254288 1101 100-101 -0.7549647538228044 1102 1 0.11524275353216495 1102 2 0.11882385300378218 1102 3 0.05035883018434362 1102 4 0.06660233589851572 1102 5 0.03223636697251919 1102 6 0.06067385932873748 1102 7 0.023944301527116352 1102 8 0.08684204667931539 1102 9 0.1296623877557579 1102 10-11 0.0587834684561912 1102 12-13 0.14775816368847217 1102 14-15 0.04625974271994693 1102 16-17 0.11024301463204011 1102 18-19 0.11912217996475505 1102 20-21 0.04084296125247988 1102 22-23 0.17657062387321076 1102 24-25 0.19700496175817506 1102 26-27 0.09952564962924271 1102 28-29 0.11524232287269598 1102 30-31 0.04949764525731126 1102 32-33 0.028812328837737766 1102 34-35 0.17093226517187077 1102 36-37 0.1915700375191136 1102 38-39 0.17953582699556137 1102 40-41 0.18258777907360724 1102 42-43 0.18811758870709383 1102 44-45 0.041732307438884675 1102 46-47 0.04665024885846236 1102 48-49 0.2377964332494713 1102 50-51 0.15973869370264993 1102 52-53 0.08920668112389052 1102 54-55 0.2730824280792916 1102 56-57 0.15983123451472636 1102 58-59 0.17046587470488817 1102 60-61 0.1491987785732718 1102 62-63 0.1676674643839675 1102 64-65 0.17051075339485777 1102 66-67 -0.01786512248121852 1102 68-69 0.15222609650773222 1102 70-71 0.1188360318151176 1102 72-73 0.2340304634026964 1102 74-75 0.08161079055695808 1102 76-77 0.19288446110241253 1102 78-79 0.024517715619289504 1102 80-81 0.10153485182686595 1102 82-83 0.09787184957186312 1102 84-85 0.12706193105575636 1102 86-87 -0.05981174010264212 1102 88-89 -0.31614729629479754 1102 90-91 -0.6799520373291621 1102 92-93 -0.866898269129404 1102 94-95 -0.8641723550876641 1102 96-97 -0.9748878808151957 1102 98-99 -1.1930600314638227 1102 100-101 -1.6694149441154096 1103 1 0.10736336504984223 1103 2 0.11214378621993148 1103 3 0.06665383604362773 1103 4 0.11522708628115907 1103 5 0.0649751028026273 1103 6 -0.007396381798635332 1103 7 4.0048994487307255E-4 1103 8 0.0022103667993675913 1103 9 0.1234156013923382 1103 10-11 0.09971342762057844 1103 12-13 0.08291174188596528 1103 14-15 0.09982994349002183 1103 16-17 0.16276093626115795 1103 18-19 0.1485645093879171 1103 20-21 0.2081450685012456 1103 22-23 0.2040695439418485 1103 24-25 0.14629751329593432 1103 26-27 0.1819536529594714 1103 28-29 0.21122318454582256 1103 30-31 0.3199122716384224 1103 32-33 0.2680286162952896 1103 34-35 0.22163252031967318 1103 36-37 0.2573578493166053 1103 38-39 0.2639875805948364 1103 40-41 0.291048480782365 1103 42-43 0.21921456729082678 1103 44-45 0.15665092396086067 1103 46-47 0.24005259690576253 1103 48-49 0.21993468613725042 1103 50-51 0.2512880065823069 1103 52-53 0.14877227593066067 1103 54-55 0.16448860334355686 1103 56-57 0.3140019929628579 1103 58-59 0.306480707506104 1103 60-61 0.34636098800073256 1103 62-63 0.24461145294043263 1103 64-65 0.08252670872681023 1103 66-67 0.03112022782011792 1103 68-69 0.10850344978653936 1103 70-71 -0.19920366108080145 1103 72-73 -0.31252102428683415 1103 74-75 -0.2292126066604787 1103 76-77 -0.10933713333963624 1103 78-79 -0.3357331871883815 1103 80-81 -0.3661315734433792 1103 82-83 -0.2858989493830322 1103 84-85 -0.49332706096844703 1103 86-87 -0.5398173153884329 1103 88-89 -0.6115440234675127 1103 90-91 -0.6446822824324769 1103 92-93 -0.655484982760008 1103 94-95 -0.7499035657766697 1103 96-97 -0.7774926827356019 1103 98-99 -0.7482094917223492 1103 100-101 -1.147952522737402 1104 1 0.20682206593559727 1104 2 0.18625508895686949 1104 3 0.22033859384497134 1104 4 0.10623494318183901 1104 5 0.08113280607913964 1104 6 0.10313011635219738 1104 7 0.10315190178697975 1104 8 0.13432007642365562 1104 9 0.20304765078427067 1104 10-11 0.21677890759792007 1104 12-13 0.20512418024627266 1104 14-15 0.30024884758017834 1104 16-17 0.19791662574315438 1104 18-19 0.20972973106041337 1104 20-21 0.1857484298927332 1104 22-23 0.10855144763707614 1104 24-25 0.24450768334828865 1104 26-27 0.2551833196847326 1104 28-29 0.1251783216084803 1104 30-31 0.06008715484433225 1104 32-33 0.04330122957870941 1104 34-35 0.18925443779091466 1104 36-37 0.1026404345200973 1104 38-39 0.20952270190777256 1104 40-41 0.3100045620663039 1104 42-43 0.31260893668433454 1104 44-45 0.3713829891533109 1104 46-47 0.40849251295805544 1104 48-49 0.2252982439848239 1104 50-51 0.22110149702825765 1104 52-53 0.3212378762531287 1104 54-55 0.21008913987166977 1104 56-57 0.3453962168682878 1104 58-59 0.1666268413961305 1104 60-61 0.16713164309076234 1104 62-63 0.049997650330027454 1104 64-65 0.15209749530032468 1104 66-67 0.1181655480834678 1104 68-69 0.23328778003070028 1104 70-71 0.11217938455075327 1104 72-73 -0.012838884908163095 1104 74-75 -0.2128743131268429 1104 76-77 -0.11728682348811859 1104 78-79 -0.13132624069479704 1104 80-81 -0.17262063217721035 1104 82-83 -0.2622312855243578 1104 84-85 -0.2893616581070475 1104 86-87 -0.4704656679717232 1104 88-89 -0.4188544005229211 1104 90-91 -0.34192149186982945 1104 92-93 -0.6497447341350231 1104 94-95 -0.8893281331990579 1104 96-97 -0.8178274395664253 1104 98-99 -0.8860034860396055 1104 100-101 -1.2846111839313963 1105 1 0.09567662308938196 1105 2 0.058496868630221854 1105 3 0.0783286301935533 1105 4 0.035535368325099626 1105 5 -7.56935982465734E-4 1105 6 0.03769412019130414 1105 7 0.06281513888787771 1105 8 0.025774302386757597 1105 9 0.01948837390138891 1105 10-11 0.10652760278832574 1105 12-13 0.07132450557146797 1105 14-15 0.05959845555249643 1105 16-17 0.07143222916761971 1105 18-19 0.10618009732183964 1105 20-21 0.09278137893175398 1105 22-23 0.13513032049247187 1105 24-25 0.027178198893409444 1105 26-27 0.03845269124988704 1105 28-29 0.06658949906712053 1105 30-31 0.05281272569089168 1105 32-33 0.11543287420963821 1105 34-35 0.13300517752343666 1105 36-37 -0.02254805543402938 1105 38-39 0.0921498896394013 1105 40-41 0.025335415833708907 1105 42-43 -0.09038476734095724 1105 44-45 -0.10024314104449417 1105 46-47 -0.19815849843740807 1105 48-49 -0.2025779669507557 1105 50-51 -0.29532396883301715 1105 52-53 -0.3251569773383167 1105 54-55 -0.2891309769423458 1105 56-57 -0.3615625232524593 1105 58-59 -0.28771948644074286 1105 60-61 -0.41678998488985997 1105 62-63 -0.26428654521282624 1105 64-65 -0.3249229651336307 1105 66-67 -0.3599872486204063 1105 68-69 -0.414161814290388 1105 70-71 -0.5087025092762012 1105 72-73 -0.3420673356054067 1105 74-75 -0.5164401118756103 1105 76-77 -0.4631828660631925 1105 78-79 -0.4417626051944836 1105 80-81 -0.577711248755385 1105 82-83 -0.6940317883504399 1105 84-85 -0.7428307810173358 1105 86-87 -0.8116938456443883 1105 88-89 -0.9547298126346639 1105 90-91 -1.1410238450135033 1105 92-93 -1.1868970249404143 1105 94-95 -1.1252785397857217 1105 96-97 -1.2812500016611885 1105 98-99 -1.3798180129721551 1105 100-101 -1.851013676624845 1106 1 0.1523666939259627 1106 2 0.20987181527375753 1106 3 0.13920940894393397 1106 4 0.08549522345953875 1106 5 0.1619693864478151 1106 6 0.15006954009219697 1106 7 0.17582363551767344 1106 8 0.12241241492599642 1106 9 0.11047840189278446 1106 10-11 0.20883280217849176 1106 12-13 0.1337224682045388 1106 14-15 0.21639485927662605 1106 16-17 0.26060775724162255 1106 18-19 0.269775925136166 1106 20-21 0.31682783336202647 1106 22-23 0.33611627999075466 1106 24-25 0.33462886191376384 1106 26-27 0.3954442586278475 1106 28-29 0.19275369016708765 1106 30-31 0.24113275007784551 1106 32-33 0.1761993391051888 1106 34-35 0.18356360520486703 1106 36-37 0.00298213882551579 1106 38-39 0.12365710831430476 1106 40-41 0.10243756061365872 1106 42-43 0.05435257482491096 1106 44-45 0.07924951346522846 1106 46-47 0.04560164529201671 1106 48-49 -0.04502317130952349 1106 50-51 0.006154370341690196 1106 52-53 -0.09141834821647166 1106 54-55 -0.1473504931477123 1106 56-57 -0.10882389133027459 1106 58-59 0.14000125331291002 1106 60-61 -0.16086806715343016 1106 62-63 -0.03621640311159524 1106 64-65 -0.01644047705607221 1106 66-67 -0.10939887730980047 1106 68-69 -0.30353450626065737 1106 70-71 -0.5843877823312944 1106 72-73 -0.5137544440829558 1106 74-75 -0.21566585803604355 1106 76-77 -0.26395922015730733 1106 78-79 -0.42818645976565506 1106 80-81 -0.42594585478963864 1106 82-83 -0.5375471224089274 1106 84-85 -0.48126037868457416 1106 86-87 -0.5061042081803286 1106 88-89 -0.587281577908616 1106 90-91 -0.8139217002335535 1106 92-93 -0.9464134783345663 1106 94-95 -0.8988621981251121 1106 96-97 -0.988240888956728 1106 98-99 -1.0362417715316923 1106 100-101 -1.6361289325938682 1107 1 -0.06263549762215348 1107 2 0.01157067465626227 1107 3 -0.04803789801068348 1107 4 -0.04338142818629365 1107 5 -0.04708994513829623 1107 6 -0.04526283979652135 1107 7 -0.025533706849635962 1107 8 0.014783010958971943 1107 9 -0.06575667491632942 1107 10-11 0.034245758507871926 1107 12-13 -0.04249401588220536 1107 14-15 -0.027671254971416204 1107 16-17 -0.11266921743895608 1107 18-19 -0.0036007875342889406 1107 20-21 0.018521225379643624 1107 22-23 0.017456255650863284 1107 24-25 -0.025522387121398538 1107 26-27 -0.044259082521179494 1107 28-29 0.005311343713977124 1107 30-31 0.013053707812908044 1107 32-33 -0.11156289421580823 1107 34-35 -0.0810739756941814 1107 36-37 -0.10953751929666566 1107 38-39 -0.08998532933518533 1107 40-41 -0.1691510291983107 1107 42-43 -0.16650825717548656 1107 44-45 -0.07901238362202179 1107 46-47 -0.06103488889199582 1107 48-49 -0.2427544725994295 1107 50-51 -0.31840147989522904 1107 52-53 -0.16896877729416104 1107 54-55 -0.1956866009692959 1107 56-57 -0.23861296701977608 1107 58-59 -0.26299674032984655 1107 60-61 -0.28302041223877694 1107 62-63 -0.4278671294335368 1107 64-65 -0.5747173403644084 1107 66-67 -0.7363783349287978 1107 68-69 -0.585480807164906 1107 70-71 -0.7539227599362697 1107 72-73 -0.87223345262327 1107 74-75 -0.6710255600223185 1107 76-77 -0.7899271784536488 1107 78-79 -0.7612588813646539 1107 80-81 -0.40360439778659796 1107 82-83 -0.5901382944002656 1107 84-85 -0.8190764296529309 1107 86-87 -0.8987846215572972 1107 88-89 -1.1338410682692128 1107 90-91 -1.2950032370527609 1107 92-93 -1.3153288028429202 1107 94-95 -1.3424033717087944 1107 96-97 -1.4550703677364503 1107 98-99 -1.9414656453971908 1107 100-101 -2.4299754042014 1108 1 0.016220709699339864 1108 2 -0.005503204845346943 1108 3 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2316 62-63 0.2389509270491459 2316 64-65 0.3511849605231774 2316 66-67 0.4422163332031488 2316 68-69 0.6809323106687906 2316 70-71 0.7311299254305901 2316 72-73 0.7647164220684459 2316 74-75 1.024504973481001 2316 76-77 0.8249262939786739 2316 78-79 0.9609178755960208 2316 80-81 0.9424302345763884 2316 82-83 0.9860968739838967 2316 84-85 1.1310370022142386 2316 86-87 1.320564750589618 2316 88-89 1.242518196516638 2316 90-91 1.439378495112873 2316 92-93 1.3554737689637548 2316 94-95 1.8955345122251437 2316 96-97 2.271503228529941 2316 98-99 2.621676169244644 2316 100-101 3.2023455920143995 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 8.0 4 16.0 5 46.0 6 89.0 7 202.0 8 620.0 9 1182.0 10 2081.0 11 3416.0 12 5360.0 13 8031.0 14 10809.0 15 15008.0 16 19755.0 17 27225.0 18 27694.0 19 22340.0 20 20398.0 21 20742.0 22 22009.0 23 24525.0 24 27123.0 25 30777.0 26 35233.0 27 41112.0 28 49699.0 29 59892.0 30 72416.0 31 87902.0 32 110009.0 33 142607.0 34 193232.0 35 273092.0 36 380123.0 37 446498.0 38 222823.0 39 9651.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.68336743259491 18.9534302697032 15.36985890446489 22.99334339323699 2 15.53991145290575 20.098657291254472 39.71604434398702 24.645386911852757 3 19.034922860452745 23.72851088936288 30.515780088069516 26.720786162114862 4 13.016417648267586 17.204931426033582 36.844048084635325 32.93460284106351 5 13.523479033263069 37.2076306858105 34.54237594293972 14.726514337986716 6 32.12331678602768 36.68385200063305 16.771071299249364 14.421759914089902 7 28.113468395817787 33.419865462287476 20.309212191667147 18.15745395022759 8 26.822819458708803 34.278779010393386 19.642572315988378 19.255829214909433 9 26.3071896101787 16.695867462497105 20.179496856961396 36.817446070362806 10-11 24.90888647401737 26.444362230175738 27.096729690394227 21.550021605412663 12-13 26.26741370027938 23.799650705786846 27.40644178737051 22.52649380656326 14-15 22.506563446790405 25.248317242859336 26.054408353485265 26.190710956864994 16-17 21.890865310120073 28.317965362405424 27.29313934998786 22.49802997748664 18-19 21.97595688363362 26.44218718863244 29.93391602351034 21.6479399042236 20-21 22.079944584084412 25.58926018344093 30.52548589392343 21.805309338551222 22-23 22.028468600893135 25.58035287045411 30.49642319596876 21.894755332683996 24-25 22.14196578462844 25.825471111825564 30.304451511651227 21.728111591894773 26-27 21.434782671753304 26.19241143843128 30.07478916921389 22.29801672060153 28-29 21.705734208134693 26.842985917371998 29.791929114324738 21.65935076016857 30-31 21.78025045718836 26.086210768184166 29.657355243020366 22.47618353160711 32-33 21.305132642319183 26.183460818387346 29.72705921540244 22.78434732389103 34-35 22.437748651141362 26.312065935176893 29.382889403949914 21.867296009731835 36-37 22.688012382525923 25.623745714091584 29.427308176079787 22.260933727302707 38-39 21.843558557354825 26.183910888986805 29.27369535977157 22.698835193886804 40-41 22.464861819168615 25.997675753049798 29.45264191474861 22.084820513032977 42-43 22.16845865885113 26.01337515373472 29.14797871857799 22.670187468836154 44-45 22.01188070081096 26.331327668419195 29.52120282722316 22.135588803546685 46-47 22.835622034169354 26.75232067056048 28.37457358740081 22.037483707869356 48-49 22.253015919114517 26.82087728409122 28.232860007121747 22.693246789672514 50-51 22.451092154547073 27.059078452457726 28.301576059294515 22.18825333370069 52-53 23.08978162569952 26.52005488205186 28.244617500562967 22.145545991685655 54-55 22.248046197270632 26.9227542274128 28.41386953932206 22.41533003599451 56-57 22.45908669952576 26.992854286702837 28.11671400779419 22.431345005977203 58-59 22.45478789476094 26.544850959543187 28.025672631268588 22.97468851442728 60-61 21.780255164990507 26.98083486696933 28.563911811717833 22.674998156322324 62-63 22.0686571604861 27.036051577196936 28.653609239374333 22.24168202294263 64-65 21.656976593609713 27.138972865378186 28.3454993631922 22.8585511778199 66-67 21.56387053253913 27.086513913200395 28.35768235767697 22.991933196583506 68-69 22.45280884161807 26.636750337346122 28.436247384945908 22.4741934360899 70-71 22.130509179349897 26.99102420747811 27.786524356753517 23.09194225641848 72-73 22.05943098818664 27.048797595979156 27.947529502329072 22.944241913505135 74-75 22.14436055144654 27.524053427521483 27.747272384102352 22.584313636929622 76-77 22.27796151115999 26.991520454629274 28.004710720411204 22.725807313799535 78-79 22.257916465938347 27.40748107973706 27.863287178769937 22.471315275554655 80-81 22.68137561496471 27.380972851536484 27.4705541006314 22.467097432867412 82-83 22.507133665305208 27.50051287789776 27.63560180781688 22.35675164898015 84-85 22.379843991122147 27.778058640906085 27.536698220948487 22.305399147023284 86-87 22.77800168465462 27.705005164823664 27.018979820407115 22.4980133301146 88-89 22.334887916868425 27.964705587404872 26.77074482225058 22.92966167347613 90-91 22.15328911816093 27.912146795256454 27.08224204408698 22.852322042495633 92-93 22.560221354841143 27.316578116718514 27.313470131641814 22.80973039679853 94-95 22.11294209195898 27.109586824562093 27.62022143728923 23.157249646189694 96-97 22.413829538617595 26.645447252705363 27.693241278949316 23.24748192972773 98-99 22.107154310792748 27.149574841699902 27.065380156406544 23.677890691100803 100-101 22.438744963175505 26.416639907187765 27.431732253286235 23.71288287635049 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 665.0 1 475.5 2 419.5 3 562.5 4 453.0 5 441.5 6 911.5 7 2126.5 8 3513.0 9 3980.5 10 3220.0 11 2352.5 12 2250.5 13 2338.5 14 2568.5 15 2789.0 16 2988.5 17 3312.5 18 3587.0 19 3970.0 20 4546.5 21 5398.0 22 6635.5 23 8174.0 24 10285.0 25 13231.0 26 16695.5 27 20645.0 28 24187.5 29 27493.0 30 31350.0 31 37386.0 32 46552.5 33 49093.5 34 46397.5 35 47344.5 36 50786.5 37 54841.5 38 58041.5 39 60690.0 40 64621.5 41 69875.5 42 72672.5 43 74313.5 44 77304.0 45 83000.5 46 85794.5 47 87969.0 48 90087.5 49 87319.0 50 84573.5 51 82156.5 52 80945.0 53 82190.0 54 85487.0 55 82149.0 56 74681.0 57 68604.5 58 63986.5 59 56631.0 60 43850.0 61 36396.5 62 32484.0 63 23733.5 64 15079.0 65 11114.0 66 8380.5 67 6578.0 68 5227.0 69 4135.0 70 3274.5 71 2160.0 72 1389.0 73 1045.5 74 753.0 75 523.5 76 369.5 77 221.0 78 124.0 79 72.5 80 42.5 81 24.0 82 14.0 83 8.5 84 8.5 85 5.0 86 1.0 87 2.0 88 2.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.008493019359526911 2 0.014168842053454651 3 0.008244443183150513 4 1.2428808818819867E-4 5 7.871578918585916E-4 6 0.0025271911264933733 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 9.321606614114902E-4 14-15 0.0 16-17 0.0028586260283285695 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.003935789459292958 26-27 0.02056967859514688 28-29 0.007063706345362624 30-31 0.003355778381081364 32-33 0.0 34-35 0.009321606614114901 36-37 9.528753427761899E-4 38-39 0.003459351787904863 40-41 0.0025686204892227727 42-43 0.0038322160524694596 44-45 2.071468136469978E-5 46-47 2.071468136469978E-4 48-49 5.59296396846894E-4 50-51 0.004163650954304656 52-53 0.008596592766350409 54-55 0.0076437174235742195 56-57 0.017068897444512617 58-59 0.02458832677989864 60-61 0.003852930733834159 62-63 0.021128974991993777 64-65 0.022309711829781663 66-67 0.013982409921172351 68-69 0.03293634336987265 70-71 0.017503905753171314 72-73 0.0051372409784455455 74-75 0.017462476390441916 76-77 0.021874703521122967 78-79 0.02732266472003901 80-81 0.01224237668653757 82-83 0.03695499155462441 84-85 0.022827578863899158 86-87 0.03270848187486095 88-89 0.023221157809828454 90-91 0.028959124547850292 92-93 0.02518905253947493 94-95 0.015639584430348335 96-97 0.016198880827195228 98-99 0.012138803279714071 100-101 0.01126878666239668 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2413747.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.7967872658974 #Duplication Level Percentage of deduplicated Percentage of total 1 72.09904583476002 35.182018016729714 2 15.060684814242538 14.698260659186493 3 5.450023850629995 7.978309632997766 4 2.5753654470864635 5.026782394136836 5 1.3416162324560315 3.2733280943815855 6 0.8511154732675953 2.491902041265146 7 0.5409985594657851 1.84792941321862 8 0.3786259653893613 1.4780584549159754 9 0.2691180631254661 1.1818887188169345 >10 1.2843731993939682 11.104209988176159 >50 0.08708444551159401 2.91849811184435 >100 0.05174414997109691 4.9277258105632304 >500 0.0048468832326367395 1.5804417785234643 >1k 0.005016949310974067 4.995721317195103 >5k 2.55099117507156E-4 0.8498261457621632 >10k+ 8.503303916905199E-5 0.465099422286416 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11209 0.46438172683383966 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9310 0.3857073670107099 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6029 0.24977762789554994 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 5142 0.21302978315457252 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 4846 0.20076669178667028 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 4419 0.18307635390121665 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 4405 0.18249634282300506 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 4169 0.17271901321886676 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 3832 0.15875731797905912 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 3460 0.14334559504372246 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 3356 0.1390369413198649 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 3179 0.1317039441167612 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 3066 0.12702242612833906 No Hit GGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCT 2811 0.11645793863234216 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 2750 0.11393074750584879 No Hit GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA 2492 0.1032419719216637 No Hit TCAGTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAG 2462 0.10199909103978172 No Hit ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT 2430 0.10067335143244094 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.285872545879912E-5 2 0.0 0.0 0.0 4.142936272939956E-5 8.285872545879912E-5 3 0.0 0.0 0.0 4.142936272939956E-5 8.285872545879912E-5 4 0.0 0.0 0.0 4.142936272939956E-5 8.285872545879912E-5 5 0.0 0.0 0.0 4.142936272939956E-5 1.2428808818819867E-4 6 0.0 0.0 0.0 4.142936272939956E-5 1.2428808818819867E-4 7 0.0 0.0 0.0 8.285872545879912E-5 1.2428808818819867E-4 8 0.0 0.0 0.0 1.2428808818819867E-4 1.2428808818819867E-4 9 0.0 0.0 0.0 1.6571745091759825E-4 1.2428808818819867E-4 10-11 0.0 4.142936272939956E-5 0.0 2.2786149501169759E-4 1.2428808818819867E-4 12-13 0.0 4.142936272939956E-5 0.0 2.6929085774109716E-4 5.385817154821943E-4 14-15 0.0 4.142936272939956E-5 0.0 2.900055391057969E-4 6.62869803670393E-4 16-17 0.0 4.142936272939956E-5 0.0 3.9357894592929584E-4 7.871578918585917E-4 18-19 0.0 4.142936272939956E-5 0.0 4.76437671388095E-4 8.700166173173907E-4 20-21 0.0 4.142936272939956E-5 0.0 6.214404409409934E-4 9.943047055055894E-4 22-23 0.0 4.142936272939956E-5 0.0 8.078725732232914E-4 9.943047055055894E-4 24-25 0.0 4.142936272939956E-5 0.0 0.001118592793693788 0.001015019386870289 26-27 4.142936272939956E-5 4.142936272939956E-5 0.0 0.0018021772787288808 0.001118592793693788 28-29 4.142936272939956E-5 4.142936272939956E-5 0.0 0.004329368405222254 0.0011393074750584878 30-31 4.142936272939956E-5 4.142936272939956E-5 0.0 0.012118088598349371 0.0011807368377878876 32-33 4.142936272939956E-5 4.142936272939956E-5 0.0 0.02937341817514429 0.0012014515191525873 34-35 4.142936272939956E-5 4.142936272939956E-5 0.0 0.05452104135188982 0.001429313014164285 36-37 4.142936272939956E-5 4.142936272939956E-5 0.0 0.0939410799889135 0.0014500276955289847 38-39 4.142936272939956E-5 4.142936272939956E-5 0.0 0.15231505207463747 0.001491457058258384 40-41 4.142936272939956E-5 4.142936272939956E-5 0.0 0.21508053660967782 0.001491457058258384 42-43 4.142936272939956E-5 4.142936272939956E-5 0.0 0.2795446250166236 0.001491457058258384 44-45 4.142936272939956E-5 4.142936272939956E-5 0.0 0.35639609287965973 0.001491457058258384 46-47 4.142936272939956E-5 4.142936272939956E-5 0.0 0.44766497897252694 0.0015121717396230838 48-49 4.142936272939956E-5 4.142936272939956E-5 0.0 0.541978923226005 0.0016778891905406823 50-51 4.142936272939956E-5 4.142936272939956E-5 0.0 0.6464223466668213 0.001781462597364181 52-53 4.142936272939956E-5 4.142936272939956E-5 0.0 0.744630651016863 0.0019057506855523797 54-55 4.142936272939956E-5 4.142936272939956E-5 0.0 0.8495919414917967 0.0020093240923758785 56-57 4.142936272939956E-5 4.142936272939956E-5 0.0 0.9692813704170321 0.002112897499199378 58-59 4.142936272939956E-5 4.142936272939956E-5 0.0 1.0954337799280538 0.002237185587387576 60-61 4.142936272939956E-5 4.142936272939956E-5 0.0 1.2297684885781317 0.002361473675575775 62-63 4.142936272939956E-5 4.142936272939956E-5 0.0 1.3630260337972455 0.0024857617637639735 64-65 4.142936272939956E-5 4.142936272939956E-5 0.0 1.4994943546278878 0.0025271911264933733 66-67 4.142936272939956E-5 4.142936272939956E-5 0.0 1.65941169476337 0.0025271911264933733 68-69 4.142936272939956E-5 4.142936272939956E-5 0.0 1.834471466976448 0.002547905807858073 70-71 4.142936272939956E-5 4.142936272939956E-5 0.0 2.0145856214425124 0.0026307645333168723 72-73 4.142936272939956E-5 4.142936272939956E-5 0.0 2.2006448894602455 0.0028379113469638698 74-75 4.142936272939956E-5 4.142936272939956E-5 0.0 2.3903085120354373 0.0030243434792461676 76-77 4.142936272939956E-5 4.142936272939956E-5 0.0 2.593519536223142 0.0030657728419755674 78-79 4.142936272939956E-5 4.142936272939956E-5 0.0 2.8253996794195912 0.0031693462487990664 80-81 4.142936272939956E-5 4.142936272939956E-5 0.0 3.0643228142800383 0.003293634336987265 82-83 6.214404409409934E-5 4.142936272939956E-5 0.0 3.314618309209706 0.0035422105133636624 84-85 8.285872545879912E-5 4.142936272939956E-5 0.0 3.5751882860962647 0.003562925194728362 86-87 8.285872545879912E-5 4.142936272939956E-5 0.0 3.8399219139371277 0.003687213282916561 88-89 8.285872545879912E-5 4.142936272939956E-5 0.0 4.111926394937001 0.0038115013711047595 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5995 0.0 51.027794 1 GTATCAA 10225 0.0 39.53451 1 TATCAAC 12855 0.0 31.48115 2 ATCAACG 12825 0.0 31.475502 3 TCAACGC 13095 0.0 30.825245 4 CAACGCA 13265 0.0 30.4302 5 AACGCAG 13635 0.0 29.569002 6 TAAGGTG 2110 0.0 27.683167 5 ACGCAGA 15190 0.0 26.416426 7 AAGGTAA 1850 0.0 26.190184 1 CGCAGAG 15260 0.0 26.07742 8 AGGTAAG 2000 0.0 25.64938 2 GTAAGGT 2420 0.0 24.529448 4 TAAGGTA 1050 0.0 23.970644 4 GGTAAGG 2330 0.0 23.8474 3 GCAGAGT 16575 0.0 23.750683 9 GTGTAAG 1150 0.0 23.54437 1 GAGTACT 11225 0.0 23.376259 12-13 GTACTTT 11640 0.0 22.31842 14-15 GTACATG 7035 0.0 21.877163 1 >>END_MODULE