FastQCFastQC Report
Thu 26 May 2016
SRR937995_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937995_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2190214
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89250.4074944274851681No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71530.3265890912942754No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44740.20427227659032404No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA31950.1458761563938501No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA28400.1296676945723112No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA27500.12555850706825908No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC24060.1098522792749932No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA23360.10665624454961936No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG22190.10131430079435161No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54900.043.1918951
GTATCAA89800.038.1475221
ATCAACG110800.030.4527553
TATCAAC112250.030.1440532
TCAACGC111550.030.12024
CAACGCA113000.029.7337025
AACGCAG116050.028.9885626
ACGCAGA129400.025.8089497
CGCAGAG131350.025.3534588
GGACCGA4850.023.5136
GCAGAGT140300.023.1583719
GTACATG64450.022.5785051
GAGTACT87600.022.39574212-13
TACATGG64450.021.7523982
AGAGTAC116950.021.60831810-11
GTACTTT91750.021.15024414-15
AGTACTT90300.020.779212-13
CAGAGTA135550.020.29030810-11
ACATGGG65250.020.1747073
TAAGGTG15050.019.2619975
Produced by FastQC (version 0.11.5)