FastQCFastQC Report
Thu 26 May 2016
SRR937995_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937995_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2190214
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84680.38662888649237015No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70860.32353002948570325No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45750.2088836981226492No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA30860.14089947375005366No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA28950.13217886471367638No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA28060.12811533484855817No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC26210.11966867164578437No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA24770.11309397163930102No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG23120.10556046121520545No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56900.040.4626271
GTATCAA87200.034.4218181
ATCAACG106400.027.7594813
TCAACGC106950.027.6154614
TATCAAC108100.027.5467012
CAACGCA108000.027.3463545
AACGCAG111650.026.4161286
TAAGGTG13100.025.0500975
GTAAGGT13450.024.0451834
ACGCAGA124600.023.5562827
CGCAGAG125500.023.3873548
CGCCTAA1252.234843E-422.8329581
GTACATG64250.021.9149021
TACATGG62650.021.7912312
GAGTACT84800.021.53460112-13
GCAGAGT137550.021.2004039
AGAGTAC112450.020.6742410-11
GGTAAGG14500.020.664933
ACATGGG64800.020.624943
GTACTTT88700.020.56098414-15
Produced by FastQC (version 0.11.5)