##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937995_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2190214 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.44494190978598 33.0 31.0 34.0 31.0 34.0 2 32.67672428356316 34.0 31.0 34.0 31.0 34.0 3 32.74511303461671 34.0 31.0 34.0 31.0 34.0 4 36.211151969624886 37.0 35.0 37.0 35.0 37.0 5 36.133436732666304 37.0 35.0 37.0 35.0 37.0 6 36.18623476975309 37.0 37.0 37.0 35.0 37.0 7 36.158664861059236 37.0 36.0 37.0 35.0 37.0 8 36.14918816152212 37.0 36.0 37.0 35.0 37.0 9 37.843499767602616 39.0 38.0 39.0 35.0 39.0 10-11 37.8083221548214 39.0 38.0 39.0 35.0 39.0 12-13 37.729895115271844 39.0 38.0 39.0 35.0 39.0 14-15 38.96139852087513 40.0 38.0 41.0 35.5 41.0 16-17 38.86310766893098 40.0 38.0 41.0 35.0 41.0 18-19 39.12202049662727 40.0 38.5 41.0 36.0 41.0 20-21 39.08962982612658 40.0 39.0 41.0 36.0 41.0 22-23 38.981708864978486 40.0 38.5 41.0 35.0 41.0 24-25 38.86844093773485 40.0 38.0 41.0 35.0 41.0 26-27 38.694956976806836 40.0 38.0 41.0 34.5 41.0 28-29 38.56514797184202 40.0 38.0 41.0 34.0 41.0 30-31 38.32178065704995 40.0 38.0 41.0 34.0 41.0 32-33 38.08819982887517 40.0 38.0 41.0 33.5 41.0 34-35 37.97885663227429 40.0 37.5 41.0 33.0 41.0 36-37 37.90961956228935 40.0 37.5 41.0 33.0 41.0 38-39 37.705503663112374 40.0 37.0 41.0 33.0 41.0 40-41 37.481035871380605 40.0 37.0 41.0 32.5 41.0 42-43 37.21560039338622 40.0 36.5 41.0 31.5 41.0 44-45 36.940643471368546 40.0 36.0 41.0 31.0 41.0 46-47 36.572287913418506 39.0 36.0 41.0 30.5 41.0 48-49 36.142088398667894 39.0 35.0 40.5 29.5 41.0 50-51 35.74758950495249 39.0 35.0 40.0 28.5 41.0 52-53 35.19651367400628 38.0 34.5 40.0 27.0 41.0 54-55 34.7946988285163 38.0 34.0 40.0 26.0 41.0 56-57 34.390257755634835 38.0 34.0 40.0 25.0 41.0 58-59 33.850964791568316 37.0 33.0 40.0 23.5 41.0 60-61 33.34867620241675 37.0 32.5 39.5 22.0 41.0 62-63 32.76525672833796 36.0 31.5 39.0 19.5 40.0 64-65 32.16840043940911 35.5 31.0 39.0 17.5 40.0 66-67 31.56578740707529 35.0 30.5 38.0 12.0 40.0 68-69 30.9008847993849 35.0 29.5 38.0 7.5 40.0 70-71 30.237372238511853 34.0 29.0 37.0 4.5 39.0 72-73 29.528537850639253 34.0 28.0 36.5 2.0 39.0 74-75 28.858133497457324 34.0 27.0 36.0 2.0 39.0 76-77 27.555688850495887 31.5 26.0 34.5 2.0 36.5 78-79 28.195196222834845 33.0 27.0 35.0 2.0 37.0 80-81 27.892181768539512 33.0 26.0 35.0 2.0 37.0 82-83 27.30046995407755 33.0 26.0 35.0 2.0 36.0 84-85 26.59497085672907 32.5 24.5 35.0 2.0 36.0 86-87 25.842309472955613 32.0 22.0 35.0 2.0 35.5 88-89 24.985827868874914 31.0 19.0 34.0 2.0 35.0 90-91 24.27047105899241 31.0 13.5 34.0 2.0 35.0 92-93 23.288440992524016 30.5 4.5 34.0 2.0 35.0 94-95 22.337603311822498 30.0 2.0 34.0 2.0 35.0 96-97 21.23686954790719 29.0 2.0 34.0 2.0 35.0 98-99 19.776041519230542 28.0 2.0 34.0 2.0 35.0 100-101 15.992406221492512 13.5 2.0 30.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2316666137054071 1101 2 0.20898932848868412 1101 3 0.210068650992298 1101 4 0.11059951583246175 1101 5 0.043316216127237794 1101 6 0.06142746228493223 1101 7 0.14248633956291457 1101 8 0.13658589082493222 1101 9 0.12020592051884194 1101 10-11 0.1832353507865605 1101 12-13 0.32223158084159564 1101 14-15 0.41052808589420664 1101 16-17 0.4425976047301319 1101 18-19 0.29888950953794335 1101 20-21 0.2930367431205525 1101 22-23 0.3981987106187148 1101 24-25 0.33457714482705114 1101 26-27 0.4963664952184459 1101 28-29 0.4587584741953137 1101 30-31 0.6130772431958533 1101 32-33 0.4951034000060872 1101 34-35 0.48757940094656504 1101 36-37 0.6136153214438238 1101 38-39 0.7822068142554386 1101 40-41 0.8871950522963061 1101 42-43 0.919722016430434 1101 44-45 0.9885207654646564 1101 46-47 1.052821460072039 1101 48-49 1.1673279441760442 1101 50-51 1.126672311971852 1101 52-53 1.2403040444255211 1101 54-55 1.2764740871127742 1101 56-57 1.2770404749761397 1101 58-59 1.4489001192060087 1101 60-61 1.4562597970734075 1101 62-63 1.2178077515228765 1101 64-65 1.4936871538744256 1101 66-67 1.7674409354917628 1101 68-69 1.9256159634602277 1101 70-71 1.9233569897496672 1101 72-73 2.093503906771762 1101 74-75 2.113078758246729 1101 76-77 2.255984842994195 1101 78-79 2.372071026711563 1101 80-81 2.461920710156839 1101 82-83 2.58551964365493 1101 84-85 2.7225306530829734 1101 86-87 3.1983222574847012 1101 88-89 3.711671038014888 1101 90-91 3.98383896703654 1101 92-93 4.256036366334932 1101 94-95 4.466647743532182 1101 96-97 4.720517646525078 1101 98-99 4.56344956039694 1101 100-101 4.22038147933131 1102 1 0.1946129437612285 1102 2 0.18115857319124018 1102 3 0.14177599607299385 1102 4 0.06728631617555436 1102 5 0.011954661696918834 1102 6 -0.0052685444970776985 1102 7 0.06177522703167426 1102 8 0.10894136121704179 1102 9 0.06152435845974935 1102 10-11 0.14347554198491963 1102 12-13 0.2469879147617391 1102 14-15 0.3390971786206265 1102 16-17 0.31600045732825777 1102 18-19 0.2088418996672914 1102 20-21 0.1890480434751325 1102 22-23 0.31477122430604965 1102 24-25 0.41433353865892997 1102 26-27 0.4886064258007039 1102 28-29 0.4198757484503375 1102 30-31 0.38891880357741115 1102 32-33 0.23050290653296912 1102 34-35 0.2885737862170643 1102 36-37 0.41514441908300626 1102 38-39 0.4490222201625329 1102 40-41 0.5391703177303242 1102 42-43 0.5871134488504808 1102 44-45 0.46593328682070023 1102 46-47 0.4113386143038582 1102 48-49 0.5678449825784853 1102 50-51 0.5191075049326201 1102 52-53 0.7427121223267719 1102 54-55 0.7869732468504793 1102 56-57 0.925663178733501 1102 58-59 0.9349293231885483 1102 60-61 1.0010115028108544 1102 62-63 1.1358318769860674 1102 64-65 1.0741780907244376 1102 66-67 1.3040714787016157 1102 68-69 1.3807991036946596 1102 70-71 1.4870872400909185 1102 72-73 1.841392766481409 1102 74-75 1.885107185373574 1102 76-77 1.879639075309992 1102 78-79 2.1102015105089293 1102 80-81 2.3036716859694693 1102 82-83 2.2134629331894438 1102 84-85 2.4657595019885363 1102 86-87 2.562027769265846 1102 88-89 2.657627519118222 1102 90-91 2.8671685725276497 1102 92-93 3.1536287128085796 1102 94-95 3.5730495137246905 1102 96-97 3.624532532597893 1102 98-99 3.6947315570159205 1102 100-101 3.5356397289296506 1103 1 0.2620361040802308 1103 2 0.20575168120167575 1103 3 0.24458399793503816 1103 4 0.15828801896003597 1103 5 0.04846726107705024 1103 6 0.12561065580673159 1103 7 0.14521436167414947 1103 8 0.20791062534463833 1103 9 0.21655271778402607 1103 10-11 0.27728059153717766 1103 12-13 0.29727885815334076 1103 14-15 0.5046353932694743 1103 16-17 0.5091036901687218 1103 18-19 0.42583078869228785 1103 20-21 0.35284108778427736 1103 22-23 0.40952174800415975 1103 24-25 0.4383469649308225 1103 26-27 0.524255260189598 1103 28-29 0.5165352283048037 1103 30-31 0.5756876781471192 1103 32-33 0.5292298607537873 1103 34-35 0.5079711965146245 1103 36-37 0.6355064139133901 1103 38-39 0.5983875348101577 1103 40-41 0.681233504509855 1103 42-43 0.726295744791976 1103 44-45 0.8473807761323613 1103 46-47 0.9235138908532008 1103 48-49 1.0684819954779883 1103 50-51 1.1860948014253694 1103 52-53 1.1879535134649402 1103 54-55 1.3230398146984754 1103 56-57 1.4274470598570943 1103 58-59 1.6434414080582584 1103 60-61 1.695508936383888 1103 62-63 1.8243380817367125 1103 64-65 1.895980224717654 1103 66-67 2.0935641959291402 1103 68-69 2.3123698305018827 1103 70-71 2.2720746835866485 1103 72-73 2.352719345844644 1103 74-75 2.4724486365379583 1103 76-77 2.5693429868142026 1103 78-79 2.5769844728639164 1103 80-81 2.8170729673454176 1103 82-83 2.803616428797728 1103 84-85 3.0124096716544706 1103 86-87 3.056098526698687 1103 88-89 3.2907145816312386 1103 90-91 3.4868482285406834 1103 92-93 3.5601817380073406 1103 94-95 3.643729157487474 1103 96-97 3.925396665096578 1103 98-99 4.293425558069437 1103 100-101 3.948597306320595 1104 1 0.1948931645657268 1104 2 0.1819564900582762 1104 3 0.1891518351027699 1104 4 0.08687707506336295 1104 5 -0.012218894720675166 1104 6 0.08953846529759346 1104 7 0.10091637401342268 1104 8 0.12181940698565086 1104 9 0.1557120327814303 1104 10-11 0.1462582770587133 1104 12-13 0.2033966001818257 1104 14-15 0.3104504195048392 1104 16-17 0.26074815767722725 1104 18-19 0.252673895421232 1104 20-21 0.21027809406923126 1104 22-23 0.22385952116912478 1104 24-25 0.3527493174649763 1104 26-27 0.34938643703328154 1104 28-29 0.38648102776279813 1104 30-31 0.2905588077094805 1104 32-33 0.38364143891212876 1104 34-35 0.3419681981867697 1104 36-37 0.49059434564377113 1104 38-39 0.5178178525175952 1104 40-41 0.5161824752761603 1104 42-43 0.575931332637218 1104 44-45 0.6973709739066791 1104 46-47 0.7673505973053736 1104 48-49 0.8706211870826763 1104 50-51 1.0957286594660829 1104 52-53 1.1468821878580684 1104 54-55 1.166599175824004 1104 56-57 1.2336766625786524 1104 58-59 1.3246297837718046 1104 60-61 1.4380932643548263 1104 62-63 1.522795672695608 1104 64-65 1.67007986145871 1104 66-67 1.8582450469929697 1104 68-69 1.8974099043068726 1104 70-71 1.865620782877432 1104 72-73 2.137716174536333 1104 74-75 2.074506527329632 1104 76-77 2.179228243113574 1104 78-79 2.1742294708458125 1104 80-81 2.4312239216209157 1104 82-83 2.662639665838608 1104 84-85 2.7881578121146013 1104 86-87 2.906322248084834 1104 88-89 3.0785373343268496 1104 90-91 3.132301149027338 1104 92-93 3.4773477415822676 1104 94-95 3.888337933362468 1104 96-97 3.9025159639491385 1104 98-99 4.368742977201077 1104 100-101 3.9423457927416283 1105 1 0.21781290118184415 1105 2 0.22569773147198902 1105 3 0.1878045033469391 1105 4 0.10135006114917644 1105 5 0.023300953699035176 1105 6 -0.0024104726229694506 1105 7 0.15140368233644352 1105 8 0.15959108041851522 1105 9 0.18819502430427093 1105 10-11 0.1666448199318964 1105 12-13 0.18482188814171963 1105 14-15 0.3449526583116267 1105 16-17 0.32379641372714474 1105 18-19 0.3149300087913858 1105 20-21 0.3011499104837938 1105 22-23 0.38199181041880337 1105 24-25 0.25522781277678064 1105 26-27 0.3294374816092258 1105 28-29 0.37539562491432577 1105 30-31 0.41211469364356645 1105 32-33 0.5691788455282278 1105 34-35 0.538763964090613 1105 36-37 0.6342336291818498 1105 38-39 0.702905091045416 1105 40-41 0.7549407990099155 1105 42-43 0.7847387646224533 1105 44-45 0.8556849713446013 1105 46-47 0.7664165668952307 1105 48-49 1.0143347510902316 1105 50-51 1.0467945677937038 1105 52-53 1.1077512584113833 1105 54-55 1.270708012574346 1105 56-57 1.3063421061884029 1105 58-59 1.3781560275320786 1105 60-61 1.6067523760741835 1105 62-63 1.7106435410430407 1105 64-65 1.6384534473675174 1105 66-67 1.8668265744576047 1105 68-69 2.0482074621313195 1105 70-71 2.2435647344890093 1105 72-73 2.3685758260198106 1105 74-75 2.310569312376831 1105 76-77 2.4066064488518784 1105 78-79 2.4158106712138228 1105 80-81 2.6286534235479415 1105 82-83 2.599897283415622 1105 84-85 2.7836018703881216 1105 86-87 2.9942167194956077 1105 88-89 3.131747639948866 1105 90-91 3.414885496743075 1105 92-93 3.531672691834043 1105 94-95 3.673021716262209 1105 96-97 4.081623569984785 1105 98-99 4.259060370317606 1105 100-101 4.123618581475499 1106 1 0.10068431527629684 1106 2 0.11922380265058052 1106 3 0.049883992353741746 1106 4 0.037959839019201524 1106 5 0.017997874460988328 1106 6 6.008885465860203E-4 1106 7 0.02764222348137224 1106 8 0.11411590524640758 1106 9 0.12198108190748513 1106 10-11 0.1463167024932588 1106 12-13 0.1772420216293753 1106 14-15 0.1219234437243486 1106 16-17 0.11143276311619843 1106 18-19 0.018891010827694288 1106 20-21 -0.0010423748489714058 1106 22-23 0.06797126522244668 1106 24-25 0.010603481088658384 1106 26-27 -0.04453136703593685 1106 28-29 0.09248153628046651 1106 30-31 0.20150059325568037 1106 32-33 0.19448855361531514 1106 34-35 0.20883015727815035 1106 36-37 0.2690672778973209 1106 38-39 0.2163269219843471 1106 40-41 0.29476143887386286 1106 42-43 0.16669145836009847 1106 44-45 0.37599159267530524 1106 46-47 0.5161488185754806 1106 48-49 0.45521526555668856 1106 50-51 0.5964324695617833 1106 52-53 0.6025151030167635 1106 54-55 0.634110306410264 1106 56-57 0.8406222565976194 1106 58-59 1.0022431047708835 1106 60-61 0.9218169120494579 1106 62-63 1.0801705313655532 1106 64-65 1.089131421904689 1106 66-67 1.2325573309405797 1106 68-69 1.293172978350288 1106 70-71 1.2373607247765825 1106 72-73 1.6715450421107576 1106 74-75 1.7230212770469748 1106 76-77 1.8533950044510838 1106 78-79 2.1540721549535498 1106 80-81 1.9366677655817277 1106 82-83 2.4093908329948626 1106 84-85 2.5146310256757936 1106 86-87 2.8018132110766523 1106 88-89 2.83444344248964 1106 90-91 2.7341669760988694 1106 92-93 2.8015218060990925 1106 94-95 3.326245761829721 1106 96-97 3.4816683623673264 1106 98-99 3.601170716299052 1106 100-101 3.4120018941422163 1107 1 0.22792036460822374 1107 2 0.22174053659584558 1107 3 0.20807034122543655 1107 4 0.12748323226048797 1107 5 0.024582178405999855 1107 6 0.09056909190794471 1107 7 0.12833622154442992 1107 8 0.13415571532362947 1107 9 0.12625948676183185 1107 10-11 0.17197845396524514 1107 12-13 0.19932622272811074 1107 14-15 0.3784843929954249 1107 16-17 0.3662602170710656 1107 18-19 0.31084586131492387 1107 20-21 0.23639206065356433 1107 22-23 0.31761665985215615 1107 24-25 0.3147452932399446 1107 26-27 0.3622782345204669 1107 28-29 0.3532101164020034 1107 30-31 0.45796158880757787 1107 32-33 0.44481198370985453 1107 34-35 0.42894292392852407 1107 36-37 0.5457156036457462 1107 38-39 0.5348696760360667 1107 40-41 0.4802314034776103 1107 42-43 0.5517942832043232 1107 44-45 0.6023736965671844 1107 46-47 0.6262865682990792 1107 48-49 0.7066705402847901 1107 50-51 0.6562676798395515 1107 52-53 0.5978032439847212 1107 54-55 0.7676776150090632 1107 56-57 0.8308221854622886 1107 58-59 0.780119265005375 1107 60-61 0.9223071459390155 1107 62-63 1.0737363186966107 1107 64-65 1.2002944203876993 1107 66-67 1.3639387277643493 1107 68-69 1.6257913158403348 1107 70-71 1.6637600768450795 1107 72-73 1.6449843499553545 1107 74-75 1.6312487644268785 1107 76-77 1.684104793701934 1107 78-79 1.5054733282580877 1107 80-81 1.7644254383420375 1107 82-83 1.9951047360965326 1107 84-85 1.9472651115010073 1107 86-87 2.317602363366266 1107 88-89 2.676101217239168 1107 90-91 2.798580666651585 1107 92-93 2.8819573386285136 1107 94-95 2.8386181681753335 1107 96-97 2.9204393708607164 1107 98-99 3.055871433651788 1107 100-101 3.047757578541292 1108 1 0.19665850787698247 1108 2 0.24456274855005944 1108 3 0.22218900808744024 1108 4 0.16817083814931522 1108 5 0.06039279377269935 1108 6 0.09827009754658178 1108 7 0.17227750231558758 1108 8 0.15720191384428972 1108 9 0.23519302896146144 1108 10-11 0.14204243805233574 1108 12-13 0.23096772964586876 1108 14-15 0.36096413559400276 1108 16-17 0.31522767186992695 1108 18-19 0.3023850857297461 1108 20-21 0.34811275644880624 1108 22-23 0.2837392786448589 1108 24-25 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0.3453650084754969 2316 58-59 0.6195980763101829 2316 60-61 0.6940059227863031 2316 62-63 0.7520714293049693 2316 64-65 0.9464757940983404 2316 66-67 1.2060433045642398 2316 68-69 1.5804378281337073 2316 70-71 1.433184910407185 2316 72-73 1.67637002087109 2316 74-75 2.008756324366132 2316 76-77 2.231019731158245 2316 78-79 2.158543002655346 2316 80-81 2.3140226824852235 2316 82-83 2.5254457456185513 2316 84-85 2.706223304179975 2316 86-87 2.7694954966015324 2316 88-89 3.109022405338205 2316 90-91 3.3026983930510845 2316 92-93 3.353770297012705 2316 94-95 3.9708675500413193 2316 96-97 4.100897524044679 2316 98-99 4.3341351365540035 2316 100-101 4.384418645114957 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 11.0 4 13.0 5 38.0 6 91.0 7 225.0 8 575.0 9 1083.0 10 1948.0 11 3151.0 12 4933.0 13 7553.0 14 9904.0 15 14069.0 16 17672.0 17 24004.0 18 25093.0 19 21639.0 20 21711.0 21 22984.0 22 24962.0 23 27804.0 24 31150.0 25 34877.0 26 39283.0 27 45424.0 28 53857.0 29 64693.0 30 76403.0 31 90913.0 32 109196.0 33 134868.0 34 172345.0 35 230205.0 36 311389.0 37 370792.0 38 187989.0 39 7366.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.6492571045569 19.015721751390505 14.946804284878098 23.38821685917449 2 15.48494538859058 19.937513898865124 40.25819125477699 24.319349457767313 3 19.31790371479709 23.6164937721445 30.07661540709354 26.988987105964863 4 12.829933681098543 16.904265640873444 37.05064663782574 33.215154040202265 5 13.408002033260818 37.34553411722974 34.754680773937324 14.491783075572117 6 32.3170247290904 36.645505872942095 16.91209169514942 14.125377702818081 7 28.416081716215857 32.74200603228726 20.4478192541916 18.394092997305286 8 26.20446377899441 35.3972934978292 19.401134230691436 18.99710849248496 9 25.904181052627735 16.75813413666427 20.245784201909036 37.09190060879896 10-11 24.873459853694662 26.510263380655953 27.268134529319966 21.34814223632942 12-13 25.814121359830594 23.831986280792652 27.676519280764346 22.67737307861241 14-15 22.318970803296303 25.42076957811866 26.087097464949753 26.17316215363529 16-17 21.844422929545456 28.55454502494848 27.143312740972515 22.45771930453355 18-19 21.938061760175035 26.924058562314002 29.523210973904835 21.61466870360613 20-21 22.21796591565938 26.14397497230864 29.857927124929347 21.78013198710263 22-23 21.998641224943018 26.069852726316405 29.813835033019693 22.11767101572088 24-25 21.9645950464843 26.32613338860982 29.765565002680116 21.943706562225767 26-27 21.522172310159384 26.651268404629253 29.63237771874375 22.194181566467616 28-29 21.66034917135951 27.100456850335174 29.40863312900018 21.830560849305137 30-31 21.81763973748684 26.59557467900397 29.32231736259562 22.26446822091357 32-33 21.467536962141597 26.607605466863056 29.436004883541063 22.488852687454287 34-35 22.247220050703937 26.768747305896706 29.16777779401343 21.816254849385928 36-37 22.348324094850504 26.250334158294354 29.22012533532828 22.18121641152687 38-39 21.812092335159004 26.699504352610536 29.17641713830411 22.311986173926346 40-41 22.295946102369243 26.516715612554957 29.1333552663272 22.0539830187486 42-43 21.958498975270658 26.55522682738453 28.961725554263623 22.52454864308119 44-45 22.028779047866912 26.773532225182468 29.162517379065672 22.035171347884948 46-47 22.575099967400448 27.035874120063152 28.303741095618967 22.085284816917433 48-49 22.151876483302544 27.081623074274937 28.36049354081382 22.406006901608702 50-51 22.254560513264916 27.307765359914605 28.348211635940597 22.089462490879885 52-53 22.685363165425844 27.029596194709743 28.19980148058592 22.0852391592785 54-55 22.093822795398076 27.34536442557668 28.320314818551978 22.240497960473267 56-57 22.246438532133965 27.320898168146623 28.161409835159905 22.271253464559503 58-59 22.264239932718905 26.98631275300039 28.107413248203144 22.642034066077564 60-61 21.868593662537087 27.244620845269 28.450553233610965 22.43623225858295 62-63 22.02147826650729 27.272472005018688 28.566089888933227 22.139959839540793 64-65 21.752121025616674 27.338995184945396 28.32654708626646 22.58233670317147 66-67 21.648059263655924 27.36805218671704 28.237397056221713 22.746491493405323 68-69 22.289202559589445 26.939519083917183 28.3915587424864 22.37971961400697 70-71 22.10973448256654 27.13775914134418 27.939187677551143 22.813318698538136 72-73 22.04965909708325 27.14220032480859 28.064895971305077 22.74324460680308 74-75 22.06633689675986 27.548769207027256 27.722884613101733 22.662009283111146 76-77 22.02545048109454 27.09148512428466 28.211558322611396 22.6715060720094 78-79 22.122244675634438 27.257930047018235 28.079950178384394 22.539875098962934 80-81 22.34800665324026 27.35578900845626 27.7430348034961 22.553169534807378 82-83 22.271112311421902 27.392076472480376 27.914478008249116 22.422333207848602 84-85 22.207867397644062 27.472836050146864 27.863703794346172 22.4555927578629 86-87 22.363927252537724 27.513044756710947 27.561032293649024 22.5619956971023 88-89 22.190913764591038 27.632048740442716 27.299638300184366 22.87739919478188 90-91 22.19985197435257 27.48912066281885 27.536012192710945 22.77501517011763 92-93 22.35201674356935 27.108446937148607 27.677021514792617 22.862514804489425 94-95 22.016762753211676 27.034448247727504 27.860623601448808 23.088165397612013 96-97 22.26375294634653 26.701952436122067 27.862479097347858 23.171815520183543 98-99 22.033600369644248 26.976861621741072 27.423393330514735 23.56614467809995 100-101 22.33204152653576 26.27966947522023 27.857369188581572 23.530919809662436 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 503.0 1 365.5 2 319.0 3 435.5 4 382.5 5 351.0 6 663.0 7 1532.5 8 2512.5 9 2899.5 10 2403.0 11 1873.5 12 1887.5 13 2009.0 14 2149.0 15 2340.5 16 2556.0 17 2786.5 18 3034.0 19 3381.5 20 3981.5 21 4818.5 22 5923.5 23 7332.0 24 9269.0 25 11766.0 26 14898.5 27 18532.0 28 21511.0 29 24452.5 30 27748.0 31 32384.5 32 39184.0 33 42602.0 34 43047.0 35 45460.0 36 49371.5 37 53670.0 38 57235.0 39 60090.0 40 63216.0 41 67382.5 42 69914.0 43 71206.5 44 73786.5 45 77787.0 46 79444.0 47 80636.0 48 82670.5 49 80589.0 50 77558.5 51 75191.0 52 73541.0 53 73666.5 54 73725.0 55 69723.5 56 63900.5 57 58662.5 58 54029.0 59 47661.5 60 37994.0 61 30948.0 62 26364.5 63 19883.0 64 13793.5 65 10242.5 66 7541.0 67 5588.5 68 4345.0 69 3345.0 70 2510.0 71 1747.0 72 1208.0 73 923.5 74 699.0 75 510.5 76 340.5 77 202.0 78 127.0 79 89.5 80 49.5 81 26.5 82 16.0 83 7.0 84 4.0 85 4.0 86 1.5 87 0.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.013103742374032858 3 0.0024655125024312693 4 0.0017806479184225832 5 0.11848157303350267 6 0.0 7 0.0 8 3.196034725373868E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 4.56576389339124E-5 16-17 2.28288194669562E-5 18-19 0.0 20-21 0.0 22-23 2.7394583360347433E-4 24-25 5.478916672069487E-4 26-27 0.006392069450747735 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.011231779177742448 36-37 4.3374756987216774E-4 38-39 0.006802988201152947 40-41 0.00961093299558856 42-43 0.00563871840833818 44-45 0.0036069534757790793 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 2.28288194669562E-5 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 6.848645840086858E-5 68-69 8.674951397443355E-4 70-71 0.0 72-73 4.56576389339124E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.3697291680173716E-4 82-83 0.001849134376823452 84-85 0.0039722145872503785 86-87 0.0028307736139025685 88-89 0.0 90-91 0.002419854863497357 92-93 0.0 94-95 4.56576389339124E-5 96-97 6.848645840086858E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2190214.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.3012139437674 #Duplication Level Percentage of deduplicated Percentage of total 1 76.63158597265071 43.91082902669064 2 13.773409691779746 15.784661909676611 3 4.350208960763158 7.4781676308235125 4 1.8464077338296878 4.232056183344068 5 0.9885545501192416 2.83226878857337 6 0.5480408821076749 1.8842044701349732 7 0.3668966697441431 1.471653719778544 8 0.25125257681272495 1.1517662126295045 9 0.18314298493343698 0.9444883825773936 >10 0.9610915094827579 9.955191233481445 >50 0.06277490120925744 2.437104773526123 >100 0.030890383243375563 3.421168203351494 >500 0.0028715916620786815 1.1219730577634839 >1k 0.002712058791963022 2.6635376204059975 >5k 1.5953287011547188E-4 0.7109287872429366 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8468 0.38662888649237015 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7086 0.32353002948570325 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4575 0.2088836981226492 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3086 0.14089947375005366 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2895 0.13217886471367638 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2806 0.12811533484855817 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2621 0.11966867164578437 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2477 0.11309397163930102 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2312 0.10556046121520545 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 4.56576389339124E-5 0.0 0.0 0.0 0.0 6 4.56576389339124E-5 0.0 0.0 0.0 0.0 7 4.56576389339124E-5 0.0 0.0 0.0 0.0 8 4.56576389339124E-5 0.0 0.0 0.0 4.56576389339124E-5 9 4.56576389339124E-5 0.0 0.0 0.0 4.56576389339124E-5 10-11 4.56576389339124E-5 0.0 0.0 0.0 4.56576389339124E-5 12-13 4.56576389339124E-5 0.0 0.0 4.56576389339124E-5 4.56576389339124E-5 14-15 4.56576389339124E-5 0.0 0.0 4.56576389339124E-5 1.1414409733478099E-4 16-17 4.56576389339124E-5 0.0 0.0 4.56576389339124E-5 1.826305557356496E-4 18-19 9.13152778678248E-5 0.0 0.0 4.56576389339124E-5 1.826305557356496E-4 20-21 9.13152778678248E-5 0.0 0.0 6.84864584008686E-5 2.0545937520260576E-4 22-23 1.1414409733478099E-4 0.0 0.0 1.598017362686934E-4 2.2828819466956196E-4 24-25 1.826305557356496E-4 0.0 0.0 3.4243229200434295E-4 2.2828819466956196E-4 26-27 1.826305557356496E-4 0.0 0.0 6.392069450747736E-4 2.2828819466956196E-4 28-29 1.826305557356496E-4 0.0 0.0 0.002899260072303437 2.5111701413651816E-4 30-31 1.826305557356496E-4 0.0 0.0 0.010820860427337237 2.739458336034744E-4 32-33 1.826305557356496E-4 0.0 0.0 0.028787141347831766 2.739458336034744E-4 34-35 1.826305557356496E-4 0.0 0.0 0.05401298685881836 2.739458336034744E-4 36-37 1.826305557356496E-4 0.0 0.0 0.09549295183027777 2.739458336034744E-4 38-39 2.2828819466956196E-4 0.0 0.0 0.1570166202937247 3.196034725373868E-4 40-41 2.2828819466956196E-4 0.0 0.0 0.22417900716550987 3.196034725373868E-4 42-43 2.2828819466956196E-4 0.0 0.0 0.28839647632605764 3.196034725373868E-4 44-45 2.739458336034744E-4 0.0 0.0 0.368457146196673 3.196034725373868E-4 46-47 3.196034725373868E-4 0.0 0.0 0.4641555574021534 3.196034725373868E-4 48-49 3.196034725373868E-4 0.0 0.0 0.5627988863188711 3.196034725373868E-4 50-51 3.196034725373868E-4 0.0 0.0 0.6684734916314115 3.196034725373868E-4 52-53 3.196034725373868E-4 0.0 0.0 0.7691257566612213 3.196034725373868E-4 54-55 3.196034725373868E-4 0.0 0.0 0.8761472623223119 3.196034725373868E-4 56-57 3.196034725373868E-4 0.0 0.0 0.9963866544547701 3.196034725373868E-4 58-59 3.196034725373868E-4 0.0 0.0 1.124136728191857 3.196034725373868E-4 60-61 3.196034725373868E-4 0.0 0.0 1.266405931109928 3.196034725373868E-4 62-63 3.4243229200434295E-4 0.0 0.0 1.401301425340172 3.652611114712992E-4 64-65 3.652611114712992E-4 0.0 0.0 1.5411964310336796 4.565763893391239E-4 66-67 3.652611114712992E-4 0.0 0.0 1.7045138055002844 4.565763893391239E-4 68-69 3.652611114712992E-4 0.0 0.0 1.8828525431761463 4.565763893391239E-4 70-71 3.652611114712992E-4 0.0 0.0 2.0656657294675314 4.565763893391239E-4 72-73 3.652611114712992E-4 0.0 0.0 2.2527707338187044 4.794052088060801E-4 74-75 3.652611114712992E-4 0.0 0.0 2.4410856656016264 5.022340282730363E-4 76-77 3.652611114712992E-4 0.0 0.0 2.639194160935872 5.478916672069488E-4 78-79 3.652611114712992E-4 0.0 0.0 2.8601543045565414 5.70720486673905E-4 80-81 4.109187504052115E-4 0.0 0.0 3.0976425134712864 6.620357645417297E-4 82-83 4.109187504052115E-4 0.0 0.0 3.3423902869765234 8.21837500810423E-4 84-85 4.109187504052115E-4 0.0 0.0 3.5880968708993732 8.446663202773793E-4 86-87 4.109187504052115E-4 0.0 0.0 3.838140930520945 8.903239592112917E-4 88-89 4.109187504052115E-4 0.0 0.0 4.1030237227960376 9.131527786782478E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5690 0.0 40.462627 1 GTATCAA 8720 0.0 34.421818 1 ATCAACG 10640 0.0 27.759481 3 TCAACGC 10695 0.0 27.615461 4 TATCAAC 10810 0.0 27.546701 2 CAACGCA 10800 0.0 27.346354 5 AACGCAG 11165 0.0 26.416128 6 TAAGGTG 1310 0.0 25.050097 5 GTAAGGT 1345 0.0 24.045183 4 ACGCAGA 12460 0.0 23.556282 7 CGCAGAG 12550 0.0 23.387354 8 CGCCTAA 125 2.234843E-4 22.832958 1 GTACATG 6425 0.0 21.914902 1 TACATGG 6265 0.0 21.791231 2 GAGTACT 8480 0.0 21.534601 12-13 GCAGAGT 13755 0.0 21.200403 9 AGAGTAC 11245 0.0 20.67424 10-11 GGTAAGG 1450 0.0 20.66493 3 ACATGGG 6480 0.0 20.62494 3 GTACTTT 8870 0.0 20.560984 14-15 >>END_MODULE