FastQCFastQC Report
Thu 26 May 2016
SRR937994_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937994_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2146632
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87980.40985133921417366No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71250.33191529801102376No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45190.2105158219946409No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA29120.13565436460464578No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA26770.12470698284568571No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA26470.1233094447487972No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA23310.10858871012823809No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC22430.10448926504403176No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54600.042.147751
GTATCAA88150.036.2887951
TATCAAC109050.029.028832
ATCAACG108750.028.9777893
TCAACGC109300.028.831974
CAACGCA110800.028.4845455
AACGCAG114150.027.6440876
ACGCAGA125700.024.9830027
CGCAGAG126350.024.892088
GTACATG63350.024.5692021
TACATGG61950.023.4779992
GAGTACT83050.023.19580312-13
GCAGAGT138100.022.3952339
GTACTTT86950.021.96493114-15
AGAGTAC111100.021.89293310-11
ACATGGG62600.021.791573
CTATACG1100.002867140821.60524
AGTACTT86800.021.34533912-13
GTGTAGC13450.020.9435251
CAGAGTA130500.020.40389810-11
Produced by FastQC (version 0.11.5)