##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937994_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2146632 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.869681435849273 33.0 31.0 34.0 30.0 34.0 2 32.0663830596022 34.0 31.0 34.0 30.0 34.0 3 32.15014031282493 34.0 31.0 34.0 30.0 34.0 4 35.66671418296196 37.0 35.0 37.0 35.0 37.0 5 35.67862912693 37.0 35.0 37.0 35.0 37.0 6 35.702464139172434 37.0 35.0 37.0 35.0 37.0 7 35.642933674705304 37.0 35.0 37.0 33.0 37.0 8 35.616939466103176 37.0 35.0 37.0 33.0 37.0 9 37.26351233001278 39.0 37.0 39.0 34.0 39.0 10-11 37.17587434641801 39.0 37.0 39.0 34.0 39.0 12-13 37.073851503191975 39.0 37.0 39.0 33.0 39.0 14-15 38.2644703423782 40.0 38.0 41.0 33.5 41.0 16-17 38.11304592496525 40.0 38.0 41.0 33.0 41.0 18-19 38.25595793783005 40.0 38.0 41.0 33.5 41.0 20-21 38.23468251661207 40.0 38.0 41.0 34.0 41.0 22-23 38.14639467780225 40.0 38.0 41.0 33.5 41.0 24-25 38.0377293360017 40.0 38.0 41.0 33.0 41.0 26-27 37.86457017318292 40.0 38.0 41.0 33.0 41.0 28-29 37.658582141699185 40.0 38.0 41.0 32.5 41.0 30-31 37.43477107394281 40.0 37.0 41.0 32.0 41.0 32-33 37.17742351739842 40.0 36.5 41.0 31.0 41.0 34-35 36.934541644771905 40.0 36.0 41.0 30.5 41.0 36-37 36.66796474663566 39.0 36.0 41.0 30.0 41.0 38-39 36.36872179302274 39.0 35.5 41.0 30.0 41.0 40-41 36.15254920265794 39.0 35.0 40.5 30.0 41.0 42-43 35.9699068121597 39.0 35.0 40.5 28.5 41.0 44-45 35.71465369937651 39.0 35.0 40.0 27.5 41.0 46-47 35.33807518009608 39.0 35.0 40.0 26.5 41.0 48-49 34.94640185183115 38.0 34.5 40.0 24.5 41.0 50-51 34.24770058398458 37.5 33.5 39.5 24.0 40.5 52-53 34.3745469647336 38.0 34.0 40.0 24.0 40.5 54-55 34.47933856385258 38.0 34.0 40.0 23.5 41.0 56-57 34.175880169493425 38.0 34.0 40.0 22.5 41.0 58-59 33.84933514454271 38.0 33.5 40.0 21.5 41.0 60-61 33.42371282082816 37.0 33.0 40.0 19.5 41.0 62-63 32.87963027663801 36.5 32.5 39.5 16.5 41.0 64-65 32.3577182768169 36.0 31.5 39.0 11.5 40.5 66-67 31.823692882617983 35.5 31.0 39.0 8.0 40.0 68-69 31.212727658955984 35.0 30.5 38.0 4.5 40.0 70-71 30.65454348952219 35.0 30.0 38.0 2.0 40.0 72-73 30.002741969746097 34.5 29.0 37.0 2.0 39.0 74-75 29.310601677418394 34.0 28.5 36.5 2.0 39.0 76-77 28.63073619511868 34.0 27.5 36.0 2.0 38.5 78-79 27.897980883542218 33.5 26.0 35.0 2.0 37.5 80-81 27.220130651178216 33.0 25.5 35.0 2.0 37.0 82-83 26.46375694576434 32.5 23.5 35.0 2.0 36.0 84-85 25.79264843717973 32.0 21.0 35.0 2.0 36.0 86-87 25.0849498190654 31.5 19.0 34.5 2.0 35.5 88-89 24.253027300440877 31.0 12.0 34.0 2.0 35.0 90-91 23.404956462029823 30.5 4.5 34.0 2.0 35.0 92-93 22.508323969828083 30.0 2.0 34.0 2.0 35.0 94-95 21.54177544171521 29.0 2.0 34.0 2.0 35.0 96-97 20.331727794983024 28.5 2.0 34.0 2.0 35.0 98-99 18.82957581923683 26.0 2.0 33.5 2.0 35.0 100-101 15.182182134618323 10.5 2.0 30.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.17974109285277606 1101 2 0.2396534865352251 1101 3 0.21581041512082066 1101 4 0.1244199430200581 1101 5 0.2357569940782298 1101 6 0.21602642357762392 1101 7 0.2005977952766287 1101 8 0.16110333461166704 1101 9 0.15304797340959908 1101 10-11 0.2804737493737335 1101 12-13 0.22111435095958853 1101 14-15 0.4967598985457684 1101 16-17 0.46951798394999855 1101 18-19 0.3954038132115514 1101 20-21 0.48085759997085376 1101 22-23 0.4801869318607572 1101 24-25 0.5526620276858694 1101 26-27 0.571744625279436 1101 28-29 0.6025544292652896 1101 30-31 0.7812304406660289 1101 32-33 0.7156725047038179 1101 34-35 0.6873823996255624 1101 36-37 0.7115892428561637 1101 38-39 0.9226205358376447 1101 40-41 0.9120999679185715 1101 42-43 1.0384531290687704 1101 44-45 0.8247891520492772 1101 46-47 0.9868826819113465 1101 48-49 1.0338256749521122 1101 50-51 1.073775628834973 1101 52-53 1.1171625196219637 1101 54-55 1.2930993202318177 1101 56-57 1.2636385674224755 1101 58-59 1.318264020931906 1101 60-61 1.4020824512042012 1101 62-63 1.4642334945668694 1101 64-65 1.3372470085322803 1101 66-67 1.3768166743721757 1101 68-69 1.7199319477137855 1101 70-71 1.928144091767468 1101 72-73 1.9612584141991967 1101 74-75 2.133386625981391 1101 76-77 2.223597596134706 1101 78-79 2.372896906275834 1101 80-81 2.631036243484715 1101 82-83 2.7506325234692355 1101 84-85 2.860969985046097 1101 86-87 2.99909277475329 1101 88-89 3.310997329518745 1101 90-91 3.364752083520134 1101 92-93 3.820487526445419 1101 94-95 4.111073383010886 1101 96-97 4.53079749770373 1101 98-99 4.66322072025817 1101 100-101 4.917755281473434 1102 1 0.38776164297671656 1102 2 0.3226138087112318 1102 3 0.18074172465106386 1102 4 0.2339585855355253 1102 5 0.3369245820194351 1102 6 0.39111876109420507 1102 7 0.19405968692034037 1102 8 0.2905508699772028 1102 9 0.4622125844636926 1102 10-11 0.4371688045001534 1102 12-13 0.4892563772834393 1102 14-15 0.6110656416572127 1102 16-17 0.5496922758890008 1102 18-19 0.560075605405622 1102 20-21 0.537463588555454 1102 22-23 0.6051666158065672 1102 24-25 0.5449362854146713 1102 26-27 0.466491019704975 1102 28-29 0.4984505263022001 1102 30-31 0.6240451594522227 1102 32-33 0.6222773529485934 1102 34-35 0.7535665224941752 1102 36-37 0.8079941715249106 1102 38-39 0.8937982668014683 1102 40-41 0.877270268668191 1102 42-43 1.011069848851264 1102 44-45 0.9324833249127877 1102 46-47 1.1068651566888335 1102 48-49 1.2678534152187098 1102 50-51 1.3056894191444854 1102 52-53 1.363911770898845 1102 54-55 1.5099931545944614 1102 56-57 1.6221057106311605 1102 58-59 1.652853288533656 1102 60-61 1.6005926872545402 1102 62-63 1.6524156128381122 1102 64-65 1.6991037218328415 1102 66-67 1.8262573676987834 1102 68-69 2.021615089289977 1102 70-71 2.1553810483805442 1102 72-73 2.3722379661056614 1102 74-75 2.362681355810878 1102 76-77 2.511036092806716 1102 78-79 2.8451726044338876 1102 80-81 2.9136621460036665 1102 82-83 3.091281556881192 1102 84-85 3.1090540340927113 1102 86-87 3.1068352019966063 1102 88-89 3.5185262131260586 1102 90-91 3.5349770905722337 1102 92-93 3.907945468912274 1102 94-95 4.178701656236306 1102 96-97 4.291702687450275 1102 98-99 4.226341050116467 1102 100-101 4.632610308271419 1103 1 0.5321099626433785 1103 2 0.4508805515376082 1103 3 0.37000726390330385 1103 4 0.3520306107780584 1103 5 0.2854752943305954 1103 6 0.3604149373215719 1103 7 0.32685930540554153 1103 8 0.30865670750574026 1103 9 0.4236734423407782 1103 10-11 0.4488783602461197 1103 12-13 0.5221620284713637 1103 14-15 0.6755839896718783 1103 16-17 0.6522272125155766 1103 18-19 0.5566793060155888 1103 20-21 0.7055803337543622 1103 22-23 0.8093200060030341 1103 24-25 0.8567054080828385 1103 26-27 0.7924444411173752 1103 28-29 0.8567771971528799 1103 30-31 0.8520700832552066 1103 32-33 0.9480059062453208 1103 34-35 1.1119578037589761 1103 36-37 1.0834670138131202 1103 38-39 1.0541777283893126 1103 40-41 1.2352525495978526 1103 42-43 1.1350931945486167 1103 44-45 1.1471368773067638 1103 46-47 1.3890012277131305 1103 48-49 1.2383137726262134 1103 50-51 1.3514674643168618 1103 52-53 1.4061237091724692 1103 54-55 1.4204849010243947 1103 56-57 1.5009525296351498 1103 58-59 1.4645760051076095 1103 60-61 1.499172690614877 1103 62-63 1.5806798488401057 1103 64-65 1.6834266688555886 1103 66-67 1.6875171722918303 1103 68-69 2.0280841883522207 1103 70-71 2.1239915782963585 1103 72-73 2.29462175912791 1103 74-75 2.3193698467712416 1103 76-77 2.354633678257347 1103 78-79 2.6564573728197267 1103 80-81 2.7955707227153255 1103 82-83 2.9803139913090817 1103 84-85 2.901326031700485 1103 86-87 2.82122359802592 1103 88-89 3.2827741523821246 1103 90-91 3.490394263453286 1103 92-93 3.8640957387092456 1103 94-95 4.0927704023138 1103 96-97 4.390180895834828 1103 98-99 4.789600912605216 1103 100-101 4.595301151468663 1104 1 0.18991486327191254 1104 2 0.12580172334175188 1104 3 0.09911848081667785 1104 4 0.0697066420470307 1104 5 0.09003564735748171 1104 6 0.09831270147731175 1104 7 0.023250897211596566 1104 8 0.07977390224692726 1104 9 -5.065625746212277E-4 1104 10-11 0.11315455705155131 1104 12-13 0.11563626066104149 1104 14-15 0.26099300358377064 1104 16-17 0.3808518492336006 1104 18-19 0.19525464549206362 1104 20-21 0.22615914927599334 1104 22-23 0.1920275905924953 1104 24-25 0.25178896431729214 1104 26-27 0.30445258458523483 1104 28-29 0.2986976790380993 1104 30-31 0.2322302855138645 1104 32-33 0.2608418422040941 1104 34-35 0.31986627488322483 1104 36-37 0.3591627738976797 1104 38-39 0.44561840803658725 1104 40-41 0.47742800391386453 1104 42-43 0.3470614141941155 1104 44-45 0.6507816825809982 1104 46-47 0.6118714887912375 1104 48-49 0.6258392175192142 1104 50-51 0.6544249406243168 1104 52-53 0.487629749269324 1104 54-55 0.8062778338741268 1104 56-57 1.1178447424784395 1104 58-59 0.8572462227627042 1104 60-61 1.0132474222793988 1104 62-63 0.9618195486041401 1104 64-65 1.1472765982656412 1104 66-67 1.2276203625606676 1104 68-69 1.3359921910810364 1104 70-71 1.5495543140783532 1104 72-73 1.657898372529754 1104 74-75 1.5344305783718433 1104 76-77 1.6095306147308044 1104 78-79 1.9642573690725627 1104 80-81 1.9973594697630546 1104 82-83 2.1791720983523177 1104 84-85 2.2631553032188485 1104 86-87 2.2379156131512303 1104 88-89 2.4655087110379057 1104 90-91 2.823775400673309 1104 92-93 3.0632697371420505 1104 94-95 3.4137285905823127 1104 96-97 3.7358502494381085 1104 98-99 3.9087915693837765 1104 100-101 4.068419111072034 1105 1 0.3578564067482368 1105 2 0.2486435049224056 1105 3 0.12866108493957995 1105 4 0.18010683558638618 1105 5 0.19327997805510932 1105 6 0.21320202116102394 1105 7 0.2599299465768681 1105 8 0.27160307193764055 1105 9 0.2512696702837829 1105 10-11 0.2332360950527459 1105 12-13 0.30125816498638613 1105 14-15 0.4563997067937251 1105 16-17 0.4589929642494468 1105 18-19 0.4069092636974929 1105 20-21 0.3001907362986742 1105 22-23 0.3481462830559252 1105 24-25 0.4598537140530894 1105 26-27 0.5503705118042603 1105 28-29 0.5059268353593254 1105 30-31 0.4948724159746618 1105 32-33 0.6014106187043424 1105 34-35 0.506053597419104 1105 36-37 0.6062027837019741 1105 38-39 0.5922738061292065 1105 40-41 0.8364347459590178 1105 42-43 0.8637712929773613 1105 44-45 0.9454218680986628 1105 46-47 0.8821302521766512 1105 48-49 0.9703734816240299 1105 50-51 0.9880220827356752 1105 52-53 0.7944481119518798 1105 54-55 0.987965684823358 1105 56-57 1.0140230565215091 1105 58-59 1.0852780739920576 1105 60-61 1.238477250258569 1105 62-63 1.345722199583932 1105 64-65 1.3290102079018595 1105 66-67 1.4279980507840513 1105 68-69 1.4916015694631959 1105 70-71 1.6208801700443338 1105 72-73 1.7429020968889724 1105 74-75 1.7506196835070007 1105 76-77 1.7657738504031606 1105 78-79 1.9055712561576321 1105 80-81 1.9111711922632786 1105 82-83 2.3724906138291004 1105 84-85 2.440547408213945 1105 86-87 2.6496447185410474 1105 88-89 2.93246337890146 1105 90-91 2.894683459932004 1105 92-93 3.1557134261039437 1105 94-95 3.295515200715112 1105 96-97 3.531497523971133 1105 98-99 3.963582225380314 1105 100-101 4.002730327441389 1106 1 0.290453462912847 1106 2 0.29366527809937537 1106 3 0.10585097632834817 1106 4 0.16710341044918664 1106 5 0.19012541396430294 1106 6 0.017522755337274987 1106 7 0.18438195645755684 1106 8 0.18390316616074642 1106 9 0.20057063670724773 1106 10-11 0.2920145211448997 1106 12-13 0.2444513414509899 1106 14-15 0.4023754093288403 1106 16-17 0.2859236625190533 1106 18-19 0.2892851661383773 1106 20-21 0.2510693826691721 1106 22-23 0.2995231022621567 1106 24-25 0.23316239340167755 1106 26-27 0.29727125603946547 1106 28-29 0.3202416646754429 1106 30-31 0.2708299264474405 1106 32-33 0.38427092658470485 1106 34-35 0.28283754956904517 1106 36-37 0.3593871904147363 1106 38-39 0.03583384789296673 1106 40-41 0.643720643052113 1106 42-43 0.4615138378924968 1106 44-45 0.4883041242327124 1106 46-47 0.6475537150030917 1106 48-49 0.7001210846646302 1106 50-51 0.598994060911572 1106 52-53 0.5257805571687868 1106 54-55 0.26318986259943955 1106 56-57 0.6564443834120155 1106 58-59 0.8045083412546248 1106 60-61 0.9133820721896342 1106 62-63 0.723489207491042 1106 64-65 0.9114148388401446 1106 66-67 0.9870458562770033 1106 68-69 0.7094212754616436 1106 70-71 1.0661611363404724 1106 72-73 1.4525572594238305 1106 74-75 1.3822761798081054 1106 76-77 1.5812541335817905 1106 78-79 1.771932413955863 1106 80-81 1.7958334374470795 1106 82-83 2.018265455623414 1106 84-85 2.0632001865222556 1106 86-87 1.934280065574928 1106 88-89 2.239296861845805 1106 90-91 2.20461023011676 1106 92-93 2.716097385256951 1106 94-95 2.6394916067403003 1106 96-97 2.8525468383070525 1106 98-99 3.353136273153975 1106 100-101 3.4196309602641346 1107 1 0.3990450156606862 1107 2 0.26175983634350786 1107 3 0.1586501319707807 1107 4 0.2391863020981333 1107 5 0.32831313952970476 1107 6 0.3562063418349055 1107 7 0.34111353285721435 1107 8 0.3447807036758377 1107 9 0.34799467209919044 1107 10-11 0.3409223862927888 1107 12-13 0.38344022632301034 1107 14-15 0.4398257772800136 1107 16-17 0.5504827798038789 1107 18-19 0.5050298613301933 1107 20-21 0.6142285391694386 1107 22-23 0.6066673599566599 1107 24-25 0.6596039440049211 1107 26-27 0.6546740905809116 1107 28-29 0.6863293418342877 1107 30-31 0.7543291850304072 1107 32-33 0.7738154912626172 1107 34-35 0.7654793532344613 1107 36-37 0.8032964648677066 1107 38-39 0.8417533888271507 1107 40-41 0.8074290455741533 1107 42-43 0.9218863233552099 1107 44-45 0.8838106621828459 1107 46-47 1.0666136472339005 1107 48-49 1.0188675996982397 1107 50-51 1.0387045253082405 1107 52-53 0.9702470432809989 1107 54-55 1.2332796598433262 1107 56-57 1.3266191219246508 1107 58-59 1.222571419912228 1107 60-61 1.3584410025883429 1107 62-63 1.50530120619203 1107 64-65 1.6879725805206505 1107 66-67 1.8028891262324542 1107 68-69 1.9383866310660771 1107 70-71 1.9383233779934024 1107 72-73 2.0426670013984527 1107 74-75 2.1523886484817076 1107 76-77 2.484473170229851 1107 78-79 2.5904774902728 1107 80-81 2.67411581623152 1107 82-83 2.730742707601035 1107 84-85 3.0140208003665343 1107 86-87 3.1833364439059473 1107 88-89 3.383508600744463 1107 90-91 3.599957072354247 1107 92-93 3.8061412575371456 1107 94-95 4.063198799098192 1107 96-97 4.27384557422166 1107 98-99 4.355593365941395 1107 100-101 4.121983044503816 1108 1 0.28192982107513487 1108 2 0.15824841324093342 1108 3 0.19136251660764003 1108 4 0.1901152836906732 1108 5 0.08439488730563482 1108 6 0.11878036304769068 1108 7 0.12620280681635876 1108 8 0.12866607044344391 1108 9 0.20788895541181063 1108 10-11 0.13425789101147956 1108 12-13 0.1831051505765089 1108 14-15 0.2942957868432714 1108 16-17 0.3465154561997039 1108 18-19 0.22668532504696515 1108 20-21 0.17228491537443347 1108 22-23 0.17238691197368894 1108 24-25 0.2793751672773439 1108 26-27 0.2580891215349084 1108 28-29 0.31263633256183 1108 30-31 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1914.0 5 2061.0 6 2474.0 7 2979.0 8 3718.0 9 4849.0 10 6111.0 11 7715.0 12 9725.0 13 11885.0 14 14291.0 15 17259.0 16 20308.0 17 24667.0 18 24134.0 19 20834.0 20 20694.0 21 22140.0 22 24318.0 23 27068.0 24 30413.0 25 34051.0 26 38531.0 27 44509.0 28 51209.0 29 59472.0 30 70235.0 31 84045.0 32 102110.0 33 129818.0 34 169547.0 35 227421.0 36 299014.0 37 336402.0 38 181556.0 39 11240.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.47545083591786 18.58137089312616 14.550769321489193 23.392408949466784 2 15.247220675240184 19.58182551130022 41.046997443790175 24.123956369669415 3 19.221542289276073 23.71183417362978 29.909069002499685 27.157554534594468 4 12.559202495023053 16.53372275729692 37.46720294808732 33.43987179959271 5 13.433798218878987 37.28903329822564 34.70118130629528 14.57598717660009 6 32.201100512957275 36.44943300550443 17.189057533086725 14.16040894845157 7 28.299433358842595 32.84193051351913 20.584109252963938 18.274526874674336 8 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29.41160980488753 22.234026835596666 32-33 21.50131430542221 26.5485500694438 29.473858371939116 22.476277253194883 34-35 22.265511481972514 26.75287127965284 29.216451710556274 21.76516552781837 36-37 22.346775415088842 26.23666763763472 29.25557821147684 22.160978735799592 38-39 21.820462124943443 26.673743256775378 29.225276473956953 22.28051814432423 40-41 22.34094800973871 26.517479584823455 29.16908775322391 21.972484652213925 42-43 21.93576029303565 26.52450716117287 29.00951825345384 22.530214292337636 44-45 22.038157919872003 26.775796012749435 29.17251202190197 22.013534045476593 46-47 22.529128014150686 27.003686783681975 28.37777324230072 22.08941195986662 48-49 22.15720637559656 27.08486909487069 28.403356293259545 22.354568236273202 50-51 22.26299563104331 27.3322573626262 28.379954330261814 22.024792676068675 52-53 22.602362361560175 27.03103898854043 28.30479836200653 22.06180028789287 54-55 22.07953649334478 27.24813890289913 28.408978927174054 22.263345676582034 56-57 22.24250017355586 27.348536982308485 28.22013685986424 22.188825984271414 58-59 22.295081203415634 26.949749744619538 28.20593041770576 22.54923863425907 60-61 21.917454303255248 27.159222296279335 28.584294917417264 22.339028483048157 62-63 22.002017924631375 27.181621836451193 28.714191563994092 22.102168674923337 64-65 21.763234098464533 27.285015061118088 28.48792431467836 22.46382652573902 66-67 21.73480676110749 27.27618757435334 28.373035423810816 22.615970240728355 68-69 22.292615563523597 26.929809276390827 28.475851118549055 22.301724041536524 70-71 22.114717743861227 27.064034343242792 28.10626425911098 22.714983653785 72-73 22.09814086071505 27.129562933136775 28.210139302657367 22.562156903490813 74-75 22.085955741568554 27.541954843289147 27.969289839775 22.402799575367297 76-77 22.20157272166293 27.122075897552488 28.102691638326665 22.573659742457917 78-79 22.335824407243994 27.303839577325018 27.997884761429976 22.362451254001016 80-81 22.58392319992843 27.434271342361182 27.592454366876524 22.389351090833863 82-83 22.462592514076583 27.471433330460165 27.765637994777048 22.300336160686204 84-85 22.480244359141967 27.57934520434468 27.705023740314367 22.235386696198987 86-87 22.697458452449084 27.55300199646269 27.395679525228083 22.353860025860143 88-89 22.55359541022169 27.61728175300305 27.2579830436918 22.571139793083457 90-91 22.567260384153325 27.577712806757397 27.285273314774035 22.569753494315243 92-93 22.809595285525884 27.160154157861545 27.463708068267245 22.566542488345323 94-95 22.498286844949742 27.018942450339473 27.673657768357206 22.809112936353582 96-97 22.86872908919768 26.66741223287761 27.593918043634265 22.869940634290455 98-99 22.717936531234635 26.986992654099655 27.103390110875804 23.191680703789906 100-101 23.50896428167358 25.896329024304045 27.344190858543854 23.250515835478517 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 530.0 1 371.0 2 335.0 3 493.5 4 424.5 5 384.0 6 662.5 7 1463.5 8 2511.0 9 2929.0 10 2402.5 11 1875.0 12 1842.0 13 1962.0 14 2121.0 15 2285.0 16 2507.5 17 2708.0 18 2986.5 19 3418.5 20 3951.5 21 4711.0 22 5830.5 23 7287.5 24 9216.5 25 11784.0 26 14798.5 27 18257.5 28 21307.5 29 24006.5 30 27120.0 31 31769.0 32 38519.0 33 41983.5 34 42425.5 35 44767.5 36 48803.0 37 52852.5 38 56227.5 39 59361.0 40 62640.5 41 66221.0 42 68240.5 43 69727.5 44 71922.0 45 75576.5 46 77526.0 47 78879.5 48 80930.0 49 79171.0 50 75869.0 51 73115.0 52 71444.5 53 71309.5 54 71434.5 55 67480.5 56 61450.5 57 56650.0 58 52772.5 59 46685.0 60 37102.5 61 30223.5 62 25894.5 63 19844.0 64 14081.0 65 10548.5 66 7790.5 67 5844.5 68 4520.0 69 3460.0 70 2645.0 71 1906.0 72 1311.0 73 986.0 74 785.5 75 554.5 76 385.5 77 249.0 78 166.5 79 111.0 80 77.5 81 54.5 82 34.0 83 22.5 84 10.0 85 6.5 86 11.0 87 10.0 88 6.5 89 4.5 90 4.5 91 3.5 92 1.5 93 1.5 94 1.0 95 0.5 96 0.5 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.46020929530539 2 0.04085469703237443 3 0.05399155514312653 4 0.05781149260795516 5 0.08399203962300013 6 0.08450447025852592 7 0.046864110848995076 8 0.04150688147758908 9 0.046724357039306226 10-11 0.05010174077345348 12-13 0.046398264816698905 14-15 0.03996958957101171 16-17 0.04064506631784116 18-19 0.04919334101047595 20-21 0.05804441562410324 22-23 0.04937967942339441 24-25 0.06903838198629295 26-27 0.05960499983229543 28-29 0.04663118783284699 30-31 0.048867248787868627 32-33 0.04714361846837278 34-35 0.0520117095058678 36-37 0.0469106954522247 38-39 0.027438331302244635 40-41 0.025947623998896878 42-43 0.025388608760141467 44-45 0.015885349701299525 46-47 0.027391746699015014 48-49 0.02673956225380037 50-51 0.016444364940054933 52-53 0.016188149622292036 54-55 0.01791177994178788 56-57 0.016258026527136465 58-59 0.015116703748010838 60-61 0.018191287561165583 62-63 0.017119841686884383 64-65 0.01756239541756575 66-67 0.009712889773375222 68-69 0.013043688904292866 70-71 0.019402487245135637 72-73 0.01723630319495843 74-75 0.014278180889877725 76-77 0.010504828028278717 78-79 0.014068550175344446 80-81 0.01788848764017307 82-83 0.015699011288381054 84-85 0.013183442713981718 86-87 0.003982983576132286 88-89 0.030093653686332822 90-91 0.03344774511886527 92-93 0.018191287561165583 94-95 0.03435614488184281 96-97 0.028509777176525832 98-99 0.025015931934304528 100-101 0.15207543724308592 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2146632.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.13471926880939 #Duplication Level Percentage of deduplicated Percentage of total 1 78.04645275440757 46.15255073558287 2 12.9998756070955 15.37487989110071 3 4.099312043132499 7.272350005976694 4 1.737943293078909 4.110911549653256 5 0.8818922365700066 2.607522491745489 6 0.4960331930710408 1.7599670172160273 7 0.3452226093615724 1.42902494628897 8 0.23979246095376686 1.1344047889022353 9 0.17546954860694 0.9338708247386491 >10 0.8891234969437188 9.454678563856442 >50 0.05550214928430943 2.224330407200904 >100 0.027859629270800296 3.149108934842928 >500 0.00268161799413109 1.0663352104570885 >1k 0.0026816179941310456 2.587412506465322 >5k 1.5774223494888502E-4 0.7426521259724445 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8798 0.40985133921417366 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7125 0.33191529801102376 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4519 0.2105158219946409 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2912 0.13565436460464578 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2677 0.12470698284568571 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2647 0.1233094447487972 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2331 0.10858871012823809 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2243 0.10448926504403176 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.6584603229617374E-5 2 0.0 0.0 0.0 0.0 4.6584603229617374E-5 3 0.0 0.0 0.0 0.0 4.6584603229617374E-5 4 0.0 0.0 0.0 0.0 4.6584603229617374E-5 5 0.0 0.0 0.0 0.0 4.6584603229617374E-5 6 0.0 0.0 0.0 0.0 4.6584603229617374E-5 7 0.0 0.0 0.0 0.0 4.6584603229617374E-5 8 0.0 0.0 0.0 0.0 4.6584603229617374E-5 9 0.0 0.0 0.0 4.6584603229617374E-5 4.6584603229617374E-5 10-11 0.0 0.0 0.0 4.6584603229617374E-5 9.316920645923475E-5 12-13 0.0 0.0 0.0 4.6584603229617374E-5 2.3292301614808686E-4 14-15 0.0 0.0 0.0 4.6584603229617374E-5 2.7950761937770426E-4 16-17 0.0 0.0 0.0 9.316920645923475E-5 3.260922226073216E-4 18-19 0.0 0.0 0.0 9.316920645923475E-5 3.260922226073216E-4 20-21 0.0 0.0 0.0 1.630461113036608E-4 3.72676825836939E-4 22-23 0.0 0.0 0.0 3.260922226073216E-4 3.72676825836939E-4 24-25 0.0 0.0 2.3292301614808687E-5 6.987690484442607E-4 3.72676825836939E-4 26-27 0.0 0.0 4.6584603229617374E-5 0.0010714458742811996 3.72676825836939E-4 28-29 0.0 0.0 4.6584603229617374E-5 0.0032376299244584075 3.72676825836939E-4 30-31 0.0 0.0 4.6584603229617374E-5 0.0115296892993303 3.72676825836939E-4 32-33 2.3292301614808687E-5 0.0 4.6584603229617374E-5 0.030559499718629 3.72676825836939E-4 34-35 4.6584603229617374E-5 0.0 4.6584603229617374E-5 0.057229185067584946 4.658460322961737E-4 36-37 4.6584603229617374E-5 0.0 4.6584603229617374E-5 0.0967795132095301 4.658460322961737E-4 38-39 4.6584603229617374E-5 0.0 4.6584603229617374E-5 0.15710657439188458 4.658460322961737E-4 40-41 4.6584603229617374E-5 0.0 4.6584603229617374E-5 0.22293061875533393 4.658460322961737E-4 42-43 4.6584603229617374E-5 0.0 4.6584603229617374E-5 0.289523309072072 5.124306355257911E-4 44-45 4.6584603229617374E-5 0.0 4.6584603229617374E-5 0.3719081798836503 5.823075403702171E-4 46-47 4.6584603229617374E-5 0.0 4.6584603229617374E-5 0.46782587793343244 6.055998419850258E-4 48-49 4.6584603229617374E-5 0.0 4.6584603229617374E-5 0.5677498518609617 6.055998419850258E-4 50-51 4.6584603229617374E-5 0.0 4.6584603229617374E-5 0.6768509926247256 6.055998419850258E-4 52-53 4.6584603229617374E-5 0.0 4.6584603229617374E-5 0.7758665667892773 6.521844452146432E-4 54-55 4.6584603229617374E-5 0.0 4.6584603229617374E-5 0.88452515382236 6.521844452146432E-4 56-57 4.6584603229617374E-5 0.0 4.6584603229617374E-5 1.0116778283376004 6.521844452146432E-4 58-59 4.6584603229617374E-5 0.0 4.6584603229617374E-5 1.1402047486481148 6.521844452146432E-4 60-61 4.6584603229617374E-5 4.6584603229617374E-5 4.6584603229617374E-5 1.2813793887354703 6.521844452146432E-4 62-63 4.6584603229617374E-5 4.6584603229617374E-5 4.6584603229617374E-5 1.4208071062017151 6.521844452146432E-4 64-65 4.6584603229617374E-5 4.6584603229617374E-5 4.6584603229617374E-5 1.5591633777936786 6.75476746829452E-4 66-67 4.6584603229617374E-5 9.316920645923475E-5 4.6584603229617374E-5 1.7210681663182137 6.987690484442606E-4 68-69 4.6584603229617374E-5 9.316920645923475E-5 4.6584603229617374E-5 1.894875320967916 7.220613500590693E-4 70-71 4.6584603229617374E-5 9.316920645923475E-5 4.6584603229617374E-5 2.0801888726153344 7.45353651673878E-4 72-73 4.6584603229617374E-5 9.316920645923475E-5 4.6584603229617374E-5 2.2663875317241144 7.45353651673878E-4 74-75 4.6584603229617374E-5 9.316920645923475E-5 4.6584603229617374E-5 2.4510488989263184 7.45353651673878E-4 76-77 4.6584603229617374E-5 9.316920645923475E-5 4.6584603229617374E-5 2.6443051254243857 7.919382549034953E-4 78-79 4.6584603229617374E-5 9.316920645923475E-5 4.6584603229617374E-5 2.8628800837777506 7.919382549034953E-4 80-81 9.316920645923475E-5 9.316920645923475E-5 4.6584603229617374E-5 3.097060884213037 7.919382549034953E-4 82-83 1.3975380968885213E-4 9.316920645923475E-5 4.6584603229617374E-5 3.3382992520376105 8.851074613627301E-4 84-85 1.3975380968885213E-4 9.316920645923475E-5 4.6584603229617374E-5 3.5826587882785685 8.851074613627301E-4 86-87 1.3975380968885213E-4 9.316920645923475E-5 4.6584603229617374E-5 3.82659906309046 9.316920645923474E-4 88-89 1.3975380968885213E-4 9.316920645923475E-5 4.6584603229617374E-5 4.087309795065013 9.782766678219648E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5460 0.0 42.14775 1 GTATCAA 8815 0.0 36.288795 1 TATCAAC 10905 0.0 29.02883 2 ATCAACG 10875 0.0 28.977789 3 TCAACGC 10930 0.0 28.83197 4 CAACGCA 11080 0.0 28.484545 5 AACGCAG 11415 0.0 27.644087 6 ACGCAGA 12570 0.0 24.983002 7 CGCAGAG 12635 0.0 24.89208 8 GTACATG 6335 0.0 24.569202 1 TACATGG 6195 0.0 23.477999 2 GAGTACT 8305 0.0 23.195803 12-13 GCAGAGT 13810 0.0 22.395233 9 GTACTTT 8695 0.0 21.964931 14-15 AGAGTAC 11110 0.0 21.892933 10-11 ACATGGG 6260 0.0 21.79157 3 CTATACG 110 0.0028671408 21.6052 4 AGTACTT 8680 0.0 21.345339 12-13 GTGTAGC 1345 0.0 20.943525 1 CAGAGTA 13050 0.0 20.403898 10-11 >>END_MODULE