FastQCFastQC Report
Thu 26 May 2016
SRR937994_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937994_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2146632
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84320.39280137443213364No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72740.33885640389223676No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46950.21871471216305355No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA30290.14110476318251103No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA28060.13071639666230636No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA26690.12433430601984877No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC25880.12056095315824977No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA24200.11273473981567403No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG22610.10532778790216489No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55050.042.889041
GTATCAA87150.035.8133771
ATCAACG107900.028.7013683
TATCAAC108750.028.612732
TCAACGC108650.028.5886824
CAACGCA110550.028.1402995
AACGCAG113800.027.4201136
TAAGGTG15650.025.4924185
GTAAGGT14800.024.7101354
ACGCAGA128100.024.3220887
CGCAGAG128800.024.1899038
AGGTAAG14050.023.3302862
GGTAAGG15400.023.1322043
GTACATG60350.022.3557031
TACATGG58000.022.2786162
GAGTACT89400.022.2598712-13
GCAGAGT141050.021.8533349
TACCCCG1353.800231E-421.1088035
GTACTTT94400.021.10600714-15
ACATGGG59850.020.7134693
Produced by FastQC (version 0.11.5)