##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937994_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2146632 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.456148981287896 33.0 31.0 34.0 31.0 34.0 2 32.690249190359594 34.0 31.0 34.0 31.0 34.0 3 32.65591028178095 34.0 31.0 34.0 31.0 34.0 4 36.19169051798352 37.0 35.0 37.0 35.0 37.0 5 36.14941126378439 37.0 35.0 37.0 35.0 37.0 6 36.21013382824816 37.0 37.0 37.0 35.0 37.0 7 36.17042697583936 37.0 37.0 37.0 35.0 37.0 8 36.163334935843686 37.0 37.0 37.0 35.0 37.0 9 37.87237635514611 39.0 38.0 39.0 35.0 39.0 10-11 37.828734501302506 39.0 38.0 39.0 35.0 39.0 12-13 37.772662710702164 39.0 38.0 39.0 35.0 39.0 14-15 39.04396375345192 40.0 38.0 41.0 36.0 41.0 16-17 38.97212004665914 40.0 38.0 41.0 36.0 41.0 18-19 38.965140275557246 40.0 38.0 41.0 35.5 41.0 20-21 38.964531880639065 40.0 38.5 41.0 35.5 41.0 22-23 38.99741641790489 40.0 38.5 41.0 35.0 41.0 24-25 38.94527590197109 40.0 38.0 41.0 35.0 41.0 26-27 38.765692955289964 40.0 38.0 41.0 35.0 41.0 28-29 38.68301925993836 40.0 38.0 41.0 34.5 41.0 30-31 38.46873381185038 40.0 38.0 41.0 34.0 41.0 32-33 38.26030055454312 40.0 38.0 41.0 34.0 41.0 34-35 38.04508737408182 40.0 38.0 41.0 33.5 41.0 36-37 37.95398815446709 40.0 37.5 41.0 33.0 41.0 38-39 37.89670446541373 40.0 38.0 41.0 33.0 41.0 40-41 37.771593361135025 40.0 37.0 41.0 33.0 41.0 42-43 37.5803211728885 40.0 37.0 41.0 33.0 41.0 44-45 37.33630147132811 40.0 37.0 41.0 32.0 41.0 46-47 36.986983097242565 40.0 36.5 41.0 31.0 41.0 48-49 36.608177833927755 39.5 36.0 41.0 30.5 41.0 50-51 36.30193274860339 39.0 35.5 41.0 30.0 41.0 52-53 35.830164881544675 39.0 35.0 40.0 28.5 41.0 54-55 35.50417747429462 38.5 35.0 40.0 28.0 41.0 56-57 35.21977311434843 38.0 34.5 40.0 27.0 41.0 58-59 34.820181801072565 38.0 34.0 40.0 26.5 41.0 60-61 34.38981972690242 37.5 34.0 40.0 26.0 41.0 62-63 33.948742728143436 37.0 33.0 40.0 25.0 41.0 64-65 33.4949183185567 36.0 33.0 39.0 23.5 40.5 66-67 32.95933723153293 36.0 32.0 39.0 22.0 40.0 68-69 32.40375271588237 35.0 31.5 38.5 20.5 40.0 70-71 31.786580792609072 35.0 31.0 37.5 18.5 40.0 72-73 31.302607992427205 35.0 30.5 37.0 17.5 39.0 74-75 30.732834971247982 34.0 30.0 36.5 13.5 39.0 76-77 29.533498988182416 33.0 28.5 35.0 13.0 37.5 78-79 30.225785789087276 34.0 30.0 36.0 10.0 38.0 80-81 30.015361971684015 34.0 30.0 35.0 7.0 37.0 82-83 29.527843617350342 34.0 29.0 35.0 4.5 37.0 84-85 28.986940239407595 34.0 29.0 35.0 2.0 36.0 86-87 28.411458973871625 33.5 28.5 35.0 2.0 36.0 88-89 27.733941821420718 33.0 26.5 35.0 2.0 35.0 90-91 27.155675728303684 33.0 26.0 35.0 2.0 35.0 92-93 26.47835260072523 32.0 25.0 34.5 2.0 35.0 94-95 25.67652653086323 32.0 23.0 34.0 2.0 35.0 96-97 24.791287235073362 31.5 19.0 34.0 2.0 35.0 98-99 23.690832895438064 31.0 2.0 34.0 2.0 35.0 100-101 20.16691845644712 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.039752761057812336 1101 2 0.06541736084626848 1101 3 0.18567489695371364 1101 4 0.07102686259494106 1101 5 -0.0639048076319284 1101 6 0.002934798529246052 1101 7 0.03936631297602844 1101 8 0.07132018838083809 1101 9 0.09326477486022355 1101 10-11 0.0862030325494203 1101 12-13 0.05044845750690996 1101 14-15 0.2017776423981701 1101 16-17 0.12593858261027435 1101 18-19 0.20144464752138447 1101 20-21 0.18219178541216507 1101 22-23 0.15988157164631644 1101 24-25 0.18807600748343845 1101 26-27 0.2435776350486094 1101 28-29 0.13356097536475176 1101 30-31 0.16847738754522368 1101 32-33 0.3125339211109335 1101 34-35 0.252731105326788 1101 36-37 0.25615978712847465 1101 38-39 0.32504479468376957 1101 40-41 0.40694524404143806 1101 42-43 0.3780282018008805 1101 44-45 0.37070760218284704 1101 46-47 0.4596557705779176 1101 48-49 0.3980461911624502 1101 50-51 0.41144325009717164 1101 52-53 0.48263121860916414 1101 54-55 0.47173694712863323 1101 56-57 0.4914283041006726 1101 58-59 0.538208835403239 1101 60-61 0.7511723171502354 1101 62-63 0.7560263964566971 1101 64-65 0.8123095289395863 1101 66-67 0.6351451741847285 1101 68-69 0.7751649468483066 1101 70-71 0.8145318895812146 1101 72-73 0.8291803610682216 1101 74-75 1.025984845470802 1101 76-77 1.0629851843710512 1101 78-79 1.0443438770173366 1101 80-81 1.228841721672353 1101 82-83 1.2486493544767931 1101 84-85 1.5154613161128658 1101 86-87 1.6929184944928792 1101 88-89 1.8871645733630231 1101 90-91 2.060154601601834 1101 92-93 2.3136627422929017 1101 94-95 2.458025999678437 1101 96-97 2.69349617237922 1101 98-99 2.9518774421101845 1101 100-101 3.568665152714889 1102 1 0.10777079043057114 1102 2 0.13027460830601 1102 3 0.20789615888976698 1102 4 0.09019801559853846 1102 5 0.08824965439775667 1102 6 0.110326481250695 1102 7 0.11334459850511536 1102 8 0.12643317204997118 1102 9 0.12919892323644433 1102 10-11 0.23748855563530213 1102 12-13 0.20778618414945527 1102 14-15 0.24210762073170855 1102 16-17 0.3078691179097959 1102 18-19 0.3090108622533805 1102 20-21 0.2562359705793398 1102 22-23 0.23791586586599323 1102 24-25 0.249205544085342 1102 26-27 0.33533717939282326 1102 28-29 0.19648948806153754 1102 30-31 0.31620216953025704 1102 32-33 0.34343256890797846 1102 34-35 0.29247338852717775 1102 36-37 0.3166988677745124 1102 38-39 0.4276095989954598 1102 40-41 0.517914472871631 1102 42-43 0.44481226345124725 1102 44-45 0.40782230236349903 1102 46-47 0.3303868484391117 1102 48-49 0.5351456468002311 1102 50-51 0.6405275572206861 1102 52-53 0.5397507202206455 1102 54-55 0.6127936220177475 1102 56-57 0.6545285879852685 1102 58-59 0.6460097200668642 1102 60-61 0.7845779120175109 1102 62-63 0.8047893678221598 1102 64-65 0.8930789582295162 1102 66-67 0.8975179212633577 1102 68-69 0.8812129491529959 1102 70-71 1.0551279872183095 1102 72-73 1.0397528716763809 1102 74-75 1.0159379715023604 1102 76-77 0.9935947860323431 1102 78-79 1.189744286339316 1102 80-81 1.290754731749356 1102 82-83 1.2857288841766454 1102 84-85 1.4375903905770535 1102 86-87 1.5807742355037036 1102 88-89 1.6104383089362209 1102 90-91 1.8578303489745522 1102 92-93 1.9286697343765447 1102 94-95 2.09122189205436 1102 96-97 2.2343318614145495 1102 98-99 2.334499143377162 1102 100-101 2.7773582632618314 1103 1 0.18283167791205557 1103 2 0.23011008303438274 1103 3 0.3555487118367324 1103 4 0.21098457352871236 1103 5 0.09100686278618753 1103 6 0.15873863182847003 1103 7 0.1691882896488366 1103 8 0.18086796820486484 1103 9 0.19434382813080475 1103 10-11 0.2913336511975402 1103 12-13 0.19062443458896894 1103 14-15 0.39741777608618634 1103 16-17 0.3942872627821572 1103 18-19 0.38406384130080085 1103 20-21 0.2952918467995147 1103 22-23 0.35561992510105966 1103 24-25 0.3798269112417074 1103 26-27 0.4273031653671353 1103 28-29 0.4387366114059503 1103 30-31 0.5234790927495112 1103 32-33 0.40750834304668615 1103 34-35 0.5192490781799322 1103 36-37 0.3771419847957631 1103 38-39 0.642677971138319 1103 40-41 0.5960101100123794 1103 42-43 0.6168168246778976 1103 44-45 0.6188161895916124 1103 46-47 0.5570092935299655 1103 48-49 0.6203570840074093 1103 50-51 0.6337486862884205 1103 52-53 0.607939178502761 1103 54-55 0.6640171462964872 1103 56-57 0.5724418775267637 1103 58-59 0.8847868314471654 1103 60-61 0.7105557152813304 1103 62-63 0.7177561556818475 1103 64-65 0.8789816522599594 1103 66-67 0.8597942431431917 1103 68-69 0.9333192337318721 1103 70-71 1.1757419025407678 1103 72-73 0.7934515567574643 1103 74-75 0.8769268003169941 1103 76-77 0.5379214097308456 1103 78-79 0.8959188036935544 1103 80-81 1.1479522871180947 1103 82-83 1.2570144046098015 1103 84-85 1.4811894383419109 1103 86-87 1.4694603402059982 1103 88-89 1.5413900695774707 1103 90-91 1.4667244126652044 1103 92-93 1.775190605330895 1103 94-95 1.7167805184697862 1103 96-97 1.8947007286850628 1103 98-99 2.33154049374377 1103 100-101 2.354086338172909 1104 1 0.009209063560199127 1104 2 0.030864309889864217 1104 3 0.10527704248643488 1104 4 -0.012157081372961898 1104 5 -0.05723836261120141 1104 6 0.03300173560396047 1104 7 -0.026300952134683087 1104 8 -0.053194739473404695 1104 9 0.01194265681159834 1104 10-11 -0.04077537913575213 1104 12-13 -0.048236295468683466 1104 14-15 -0.04650936693312957 1104 16-17 -0.03156091868635258 1104 18-19 0.04687120513825249 1104 20-21 0.029619473423331044 1104 22-23 0.09579464210755617 1104 24-25 0.06479309860208105 1104 26-27 0.0684152793955235 1104 28-29 0.02922303179580865 1104 30-31 0.05565317494243516 1104 32-33 0.14669543450943223 1104 34-35 0.08246524101843278 1104 36-37 0.04496090422403398 1104 38-39 0.14368942974373056 1104 40-41 0.1444274569545314 1104 42-43 0.20442922580513567 1104 44-45 0.20910698600712863 1104 46-47 0.31413634331102713 1104 48-49 0.20045104964151506 1104 50-51 0.15278165912258856 1104 52-53 0.2746875388432244 1104 54-55 0.3426157732917119 1104 56-57 0.5211627722612278 1104 58-59 0.2912528099582872 1104 60-61 0.28797252549742325 1104 62-63 0.28731149963288516 1104 64-65 0.4042357430052377 1104 66-67 0.28052851084274977 1104 68-69 0.6102969370072273 1104 70-71 0.6943421606541982 1104 72-73 0.5654485083297836 1104 74-75 0.6310288421904424 1104 76-77 0.6710709391863077 1104 78-79 0.8246330141496863 1104 80-81 0.8530994294100118 1104 82-83 1.0569883397816717 1104 84-85 1.3134283795257709 1104 86-87 1.2657039190556816 1104 88-89 1.3037747203142835 1104 90-91 1.2645806494330323 1104 92-93 1.4590928462448503 1104 94-95 1.5696485588024913 1104 96-97 1.8118450209285442 1104 98-99 2.219967186552413 1104 100-101 2.7096224415417147 1105 1 0.10928848472474328 1105 2 0.11484144805404384 1105 3 0.16217936241207553 1105 4 0.08174133593352906 1105 5 -0.006186788194050052 1105 6 0.04760711268737339 1105 7 0.04057198673489637 1105 8 0.07216337198997991 1105 9 0.03236774307666934 1105 10-11 0.026561910931349075 1105 12-13 0.034770758531337265 1105 14-15 0.10197399122926498 1105 16-17 0.04871931547079811 1105 18-19 0.09687510457418824 1105 20-21 0.14120183269348985 1105 22-23 0.1472114902173658 1105 24-25 0.188148571181884 1105 26-27 0.15540453286840972 1105 28-29 0.023985850594591795 1105 30-31 0.03730553306663609 1105 32-33 0.10924294396357936 1105 34-35 0.07126669765670357 1105 36-37 0.1428116783814346 1105 38-39 0.03328684879462429 1105 40-41 0.06323563017225098 1105 42-43 0.010308540650363796 1105 44-45 0.15353922813503829 1105 46-47 0.12216233742845617 1105 48-49 0.18758848167991715 1105 50-51 0.12408181064352419 1105 52-53 0.2024381532033317 1105 54-55 0.21146540785886003 1105 56-57 0.2567467963517842 1105 58-59 0.224987466871589 1105 60-61 0.3057730526374911 1105 62-63 0.4033149438693542 1105 64-65 0.44797507924165814 1105 66-67 0.6027436769428007 1105 68-69 0.6202102581170195 1105 70-71 0.7799065284044282 1105 72-73 0.7079080337763877 1105 74-75 0.694764724640752 1105 76-77 0.7151831092462757 1105 78-79 0.9104783004478634 1105 80-81 1.1277920106137778 1105 82-83 1.0508292205130445 1105 84-85 1.312901557772964 1105 86-87 1.469185239961753 1105 88-89 1.5688233598090413 1105 90-91 1.6206556522979234 1105 92-93 1.766961271318383 1105 94-95 1.7273604493763592 1105 96-97 1.8904954500256608 1105 98-99 2.218156336252555 1105 100-101 2.619246647330069 1106 1 0.07294335440400346 1106 2 0.12152858278034984 1106 3 0.13579768880600795 1106 4 0.08838151826797258 1106 5 0.07651388155396432 1106 6 0.03838773201329815 1106 7 0.08680497246136554 1106 8 0.017272046882069958 1106 9 0.09766976632685953 1106 10-11 0.08871295120536615 1106 12-13 0.023352573472067206 1106 14-15 0.08163246430564897 1106 16-17 0.16749653194201386 1106 18-19 0.1254169861077301 1106 20-21 0.02423347701400047 1106 22-23 0.023981356649748875 1106 24-25 0.09823115964337603 1106 26-27 0.12182641045477283 1106 28-29 0.14749053628413833 1106 30-31 0.17594042808427446 1106 32-33 0.17946024599955734 1106 34-35 0.10737547441161155 1106 36-37 0.16008657747358512 1106 38-39 0.3061669880920235 1106 40-41 0.33518149645183115 1106 42-43 0.27220301395593793 1106 44-45 0.46763481365523774 1106 46-47 0.25466596629127736 1106 48-49 0.5209178359969897 1106 50-51 0.5416954831800425 1106 52-53 0.373430806347713 1106 54-55 0.5495278020170318 1106 56-57 0.6475092713635675 1106 58-59 0.6000499374268671 1106 60-61 0.7121197427326109 1106 62-63 0.6089003685736358 1106 64-65 0.4940023498275039 1106 66-67 0.44659673346393447 1106 68-69 0.3948570806337912 1106 70-71 0.6539471875841834 1106 72-73 0.5867680774500705 1106 74-75 0.5762712120288604 1106 76-77 0.7051822497787654 1106 78-79 1.0055127268965443 1106 80-81 0.8748678315644121 1106 82-83 0.9735053954369661 1106 84-85 0.9799454351810688 1106 86-87 1.1573107413178008 1106 88-89 1.3266652050539598 1106 90-91 1.3307835219644488 1106 92-93 1.4415883579145081 1106 94-95 1.7554654349245702 1106 96-97 1.9056511250578083 1106 98-99 2.2691144037876008 1106 100-101 2.2681951524932416 1107 1 0.13430633624847843 1107 2 0.12552059263322946 1107 3 0.20228931569554476 1107 4 0.08408926515677706 1107 5 0.08452164498678627 1107 6 0.08496066967911986 1107 7 0.06408758715122786 1107 8 0.08877436610805489 1107 9 0.182060333786616 1107 10-11 0.13413776477881356 1107 12-13 0.1717267409097687 1107 14-15 0.167799215734874 1107 16-17 0.21229903008847373 1107 18-19 0.20392600259660298 1107 20-21 0.265276730425434 1107 22-23 0.12930852989011754 1107 24-25 0.17509956118503567 1107 26-27 0.19580343878180884 1107 28-29 0.17676463693302935 1107 30-31 0.25821435716558483 1107 32-33 0.10825127669177448 1107 34-35 0.1101557882577282 1107 36-37 0.28665940790966715 1107 38-39 0.3425266764517758 1107 40-41 0.44483615543064303 1107 42-43 0.43997580640206735 1107 44-45 0.27712472180530767 1107 46-47 0.33076193077679505 1107 48-49 0.5275768025474434 1107 50-51 0.45558158069169963 1107 52-53 0.28414366962672943 1107 54-55 0.4036907667047416 1107 56-57 0.44670818864960893 1107 58-59 0.3772874390353138 1107 60-61 0.5172863777409731 1107 62-63 0.6708079242871392 1107 64-65 0.5918333986568527 1107 66-67 0.6490441755751917 1107 68-69 0.738927429467445 1107 70-71 0.8487158317714112 1107 72-73 0.6796776644011331 1107 74-75 0.8809591428626184 1107 76-77 0.9837229756321619 1107 78-79 1.1327048090528997 1107 80-81 1.1105645337904981 1107 82-83 1.2025603644628937 1107 84-85 1.475637863536285 1107 86-87 1.5791218821809103 1107 88-89 1.6274913335703296 1107 90-91 1.5728967749469653 1107 92-93 1.7752486142854842 1107 94-95 2.031383244864461 1107 96-97 1.9596769122772493 1107 98-99 2.303251372923821 1107 100-101 2.4208458715452075 1108 1 0.04487901773698866 1108 2 0.05412276541714789 1108 3 0.15436993931838572 1108 4 0.011511583574225881 1108 5 -0.009010025892436602 1108 6 0.04996435175068825 1108 7 0.05155226747652364 1108 8 0.04475588775125772 1108 9 0.0108427399950628 1108 10-11 0.0642115002512611 1108 12-13 0.06515274212218003 1108 14-15 0.15889967045985998 1108 16-17 0.08298064374729108 1108 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-3.1592815414083013 2316 96-97 -3.3737586833257467 2316 98-99 -3.9535971195002553 2316 100-101 -4.489137788230305 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 6.0 4 11.0 5 37.0 6 70.0 7 199.0 8 501.0 9 969.0 10 1678.0 11 2646.0 12 4111.0 13 6090.0 14 8730.0 15 11399.0 16 14468.0 17 19606.0 18 20496.0 19 15904.0 20 14725.0 21 14862.0 22 15798.0 23 17300.0 24 19899.0 25 22487.0 26 26306.0 27 31358.0 28 37809.0 29 46039.0 30 56046.0 31 68857.0 32 86016.0 33 112057.0 34 154059.0 35 224825.0 36 338238.0 37 455797.0 38 280243.0 39 16985.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.66539397530643 18.99529122830555 14.966188894975943 23.373125901412074 2 15.490380243158826 19.93965755007417 40.33104486735097 24.23891733941603 3 19.515749418120357 23.645138206897578 30.024104445765467 26.8150079292166 4 12.900021987932725 16.871499167067295 37.12993191194392 33.09854693305606 5 13.444773332662512 37.36193449627462 34.71075073161324 14.482541439449628 6 32.39294372383105 36.590829896936036 16.90320819443332 14.113018184799603 7 28.40552083449795 32.795793596666776 20.453296140186115 18.34538942864916 8 26.110961781507864 35.42690849056033 19.432450197542103 19.02967953038971 9 25.903194354316135 16.808105352060974 20.260957751937802 37.027742541685086 10-11 24.914307622359118 26.481017705876 27.324268901236913 21.28040577052797 12-13 25.88555001509341 23.798839298025932 27.668319488389255 22.647291198491402 14-15 22.338481863682272 25.412320323185345 26.117098785446224 26.132099027686163 16-17 21.898099417622447 28.52320018596578 27.166795977791253 22.411904418620523 18-19 21.924810587003268 26.886932646117266 29.494156427370875 21.694100339508587 20-21 22.162229017363014 26.11891558497218 29.936826619560314 21.782028778104493 22-23 22.008266903689126 26.0612205538723 29.847547227470756 22.08296531496782 24-25 21.934057907043968 26.29498664280252 29.861611095336777 21.90934435481673 26-27 21.548894108021127 26.615726155066938 29.621461738586085 22.21391799832585 28-29 21.637475528474315 27.125106708080466 29.427019825284077 21.810397938161138 30-31 21.81582294107508 26.55143177911424 29.3437387905694 22.289006489241274 32-33 21.518719028920238 26.55645617008431 29.448107412840734 22.476717388154714 34-35 22.255439733009176 26.712360998578475 29.17947752657957 21.85272174183277 36-37 22.34439197061963 26.270494724586964 29.224884426362017 22.160228878431397 38-39 21.809825319829958 26.665779819441944 29.225863913060397 22.298530947667704 40-41 22.25681396177798 26.506480223843216 29.152526972781427 22.084178841597378 42-43 21.89783283536887 26.556869871111655 28.997909131678114 22.54738816184136 44-45 22.022769869825893 26.759838299168898 29.175939803385432 22.041452027619776 46-47 22.493582845350065 27.016547174821458 28.426760583064304 22.06310939676417 48-49 22.14521515949313 27.064254480922063 28.400699049695838 22.389831309888972 50-51 22.216989218459428 27.31225007360367 28.414278739905118 22.056481968031783 52-53 22.612911761307945 26.986087974091504 28.296606032147103 22.10439423245344 54-55 22.08690171394072 27.241394892091424 28.422990060709054 22.2487133332588 56-57 22.242820380950253 27.35559704690883 28.175392894543638 22.226189677597276 58-59 22.248643456353953 27.023774918104266 28.13859105799224 22.588990567549537 60-61 21.865438510187122 27.25492771932963 28.499435394608856 22.380198375874393 62-63 21.966294176179243 27.215470560394145 28.66206224448345 22.15617301894316 64-65 21.674115544723083 27.36335338334656 28.411460371409724 22.55107070052063 66-67 21.599845152382123 27.303399077575907 28.340281832913178 22.756473937128792 68-69 22.20886017718491 26.959195295876903 28.45456419430013 22.377380332638054 70-71 22.038290680470617 27.117642893611947 28.04791412780579 22.796152298111647 72-73 21.994431276529934 27.214771791345697 28.058116155913076 22.732680776211293 74-75 22.007381796227765 27.6054768586325 27.79875637743218 22.588384967707555 76-77 21.99543284549937 27.14202993340265 28.160532406113393 22.70200481498459 78-79 22.12034945905959 27.311504719952 28.080150673240684 22.48799514774773 80-81 22.335464506750476 27.35701809327333 27.74590735742804 22.561610042548157 82-83 22.21419405811816 27.482129376581998 27.878731553177232 22.424945012122606 84-85 22.182781590234168 27.53840997081124 27.854895225840774 22.423913213113813 86-87 22.359226037272638 27.54012713038901 27.591743279535454 22.5089035528029 88-89 22.177239590782023 27.630831754502754 27.35682099065932 22.835107664055908 90-91 22.127998565137737 27.564239785050184 27.53360920268478 22.774152447127307 92-93 22.33298557708321 27.201829295703917 27.729659462079546 22.735525665133324 94-95 21.96620841328427 27.105056346485583 27.95264109447883 22.976094145751315 96-97 22.252370287811928 26.76307370514912 27.941200790542375 23.043355216496575 98-99 22.029038100686286 27.072925537168064 27.501434808453073 23.39660155369258 100-101 22.257192684061366 26.551268801184776 27.851643561238408 23.339894953515454 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 501.0 1 368.0 2 351.0 3 468.5 4 392.0 5 380.0 6 714.5 7 1537.5 8 2546.5 9 2985.5 10 2499.5 11 1933.0 12 1878.0 13 1968.5 14 2149.0 15 2366.0 16 2527.5 17 2751.0 18 3011.0 19 3320.5 20 3835.0 21 4561.0 22 5627.5 23 7069.5 24 8998.5 25 11326.5 26 14327.0 27 17788.0 28 20868.0 29 23974.0 30 27144.5 31 31495.5 32 38318.0 33 42031.5 34 42361.5 35 44596.0 36 48678.0 37 52892.0 38 56521.5 39 59240.5 40 62486.0 41 66371.0 42 68290.5 43 69740.5 44 72060.0 45 76157.5 46 77859.5 47 79491.5 48 81949.0 49 79382.5 50 75955.0 51 74086.5 52 72857.5 53 72857.0 54 73315.5 55 68761.0 56 62325.0 57 57378.0 58 53009.0 59 46567.5 60 36508.5 61 29703.5 62 25350.5 63 18758.5 64 12851.0 65 9576.5 66 7056.0 67 5327.5 68 4016.5 69 2964.0 70 2233.5 71 1554.0 72 1049.0 73 826.0 74 630.5 75 448.0 76 335.0 77 204.5 78 107.5 79 67.5 80 44.5 81 25.5 82 17.5 83 7.5 84 6.5 85 5.0 86 3.0 87 1.5 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.010062274297597353 3 0.006521844452146433 4 0.0 5 2.795076193777042E-4 6 1.863384129184695E-4 7 0.0 8 4.6584603229617374E-5 9 4.6584603229617374E-5 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.3292301614808687E-5 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0017003380178810341 26-27 0.021824886613075738 28-29 2.0963071453327815E-4 30-31 9.316920645923475E-5 32-33 0.0034006760357620683 34-35 0.015885349701299525 36-37 0.007686459532886867 38-39 0.021079532961401862 40-41 0.022686701772823663 42-43 0.018377625974084055 44-45 0.009270336042693858 46-47 0.0019565533356439295 48-49 1.630461113036608E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.5621531776289554E-4 68-69 2.0963071453327815E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.5621531776289554E-4 82-83 0.0010481535726663909 84-85 0.004029568179361903 86-87 7.919382549034954E-4 88-89 4.6584603229617374E-5 90-91 0.003936398972902668 92-93 6.288921435998345E-4 94-95 1.397538096888521E-4 96-97 2.0963071453327815E-4 98-99 1.863384129184695E-4 100-101 0.0027251992889326163 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2146632.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.668909148044364 #Duplication Level Percentage of deduplicated Percentage of total 1 76.85417421129769 44.320963902393004 2 13.658103216492758 15.75295827053067 3 4.318583245008255 7.47143954413943 4 1.8130694671509073 4.182309535208755 5 0.9500736218872377 2.7394854692284283 6 0.5737714226176495 1.9853263225608837 7 0.36631977732523446 1.4787683370390552 8 0.24968808655808794 1.151939166325393 9 0.17600957283684351 0.9135252058602624 >10 0.9433989620561624 9.820255856462966 >50 0.06060807758269977 2.369571154867778 >100 0.030547207216345646 3.34809889559474 >500 0.002745806956935256 1.0722467386701704 >1k 0.002745806956935147 2.661638410068124 >5k 1.6151805629030278E-4 0.7314731910503929 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8432 0.39280137443213364 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7274 0.33885640389223676 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4695 0.21871471216305355 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3029 0.14110476318251103 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2806 0.13071639666230636 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2669 0.12433430601984877 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2588 0.12056095315824977 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2420 0.11273473981567403 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2261 0.10532778790216489 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.6584603229617374E-5 0.0 5 0.0 0.0 0.0 4.6584603229617374E-5 0.0 6 0.0 0.0 0.0 4.6584603229617374E-5 0.0 7 0.0 0.0 0.0 4.6584603229617374E-5 0.0 8 0.0 0.0 0.0 4.6584603229617374E-5 0.0 9 0.0 0.0 0.0 4.6584603229617374E-5 0.0 10-11 0.0 0.0 0.0 4.6584603229617374E-5 0.0 12-13 0.0 0.0 0.0 4.6584603229617374E-5 6.987690484442606E-5 14-15 0.0 0.0 0.0 4.6584603229617374E-5 1.863384129184695E-4 16-17 0.0 0.0 0.0 9.316920645923475E-5 3.260922226073216E-4 18-19 0.0 0.0 0.0 9.316920645923475E-5 3.260922226073216E-4 20-21 0.0 0.0 0.0 1.630461113036608E-4 3.4938452422213033E-4 22-23 0.0 0.0 0.0 3.260922226073216E-4 4.1926142906655636E-4 24-25 0.0 0.0 0.0 6.987690484442607E-4 4.1926142906655636E-4 26-27 0.0 0.0 0.0 0.0010714458742811996 4.1926142906655636E-4 28-29 9.316920645923475E-5 0.0 0.0 0.003260922226073216 4.658460322961737E-4 30-31 9.316920645923475E-5 0.0 0.0 0.011552981600945108 4.658460322961737E-4 32-33 9.316920645923475E-5 0.0 0.0 0.030629376623473422 4.658460322961737E-4 34-35 9.316920645923475E-5 0.0 0.0 0.05720589276597013 4.658460322961737E-4 36-37 9.316920645923475E-5 0.0 0.0 0.09708231313052261 4.658460322961737E-4 38-39 1.3975380968885213E-4 0.0 0.0 0.1579683895516325 4.658460322961737E-4 40-41 1.3975380968885213E-4 0.0 0.0 0.22409523383607438 4.658460322961737E-4 42-43 1.3975380968885213E-4 0.0 0.0 0.2910838932802642 4.658460322961737E-4 44-45 1.630461113036608E-4 0.0 0.0 0.3738181486160646 4.658460322961737E-4 46-47 2.3292301614808686E-4 0.0 0.0 0.4695262159513135 4.658460322961737E-4 48-49 2.3292301614808686E-4 0.0 0.0 0.569054220751391 4.658460322961737E-4 50-51 2.3292301614808686E-4 0.0 0.0 0.6779690231022364 5.124306355257911E-4 52-53 2.3292301614808686E-4 0.0 0.0 0.7770311818700177 5.124306355257911E-4 54-55 2.3292301614808686E-4 0.0 0.0 0.8857363535063298 6.055998419850258E-4 56-57 2.3292301614808686E-4 0.0 0.0 1.0127492742118818 6.055998419850258E-4 58-59 2.3292301614808686E-4 0.0 0.0 1.1414625329353145 6.055998419850258E-4 60-61 2.3292301614808686E-4 0.0 0.0 1.282497419212981 6.521844452146432E-4 62-63 2.3292301614808686E-4 0.0 0.0 1.421901844377611 6.521844452146432E-4 64-65 2.3292301614808686E-4 0.0 0.0 1.560467746684108 6.521844452146432E-4 66-67 2.7950761937770426E-4 0.0 0.0 1.7225588736215616 6.521844452146432E-4 68-69 2.7950761937770426E-4 0.0 0.0 1.896482489779338 6.521844452146432E-4 70-71 2.7950761937770426E-4 0.0 0.0 2.082774318094578 6.521844452146432E-4 72-73 2.7950761937770426E-4 0.0 0.0 2.2709528228406173 6.521844452146432E-4 74-75 2.7950761937770426E-4 0.0 0.0 2.457035020441324 6.75476746829452E-4 76-77 2.7950761937770426E-4 0.0 0.0 2.6528534001170208 6.987690484442606E-4 78-79 2.7950761937770426E-4 0.0 0.0 2.8749687883158366 6.987690484442606E-4 80-81 2.7950761937770426E-4 0.0 0.0 3.1123406340723516 6.987690484442606E-4 82-83 2.7950761937770426E-4 0.0 0.0 3.3580278315053533 6.987690484442606E-4 84-85 2.7950761937770426E-4 0.0 0.0 3.606719735846666 6.987690484442606E-4 86-87 2.7950761937770426E-4 0.0 0.0 3.8560172400299635 7.686459532886866E-4 88-89 2.7950761937770426E-4 0.0 0.0 4.122714093519523 8.385228581331127E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5505 0.0 42.88904 1 GTATCAA 8715 0.0 35.813377 1 ATCAACG 10790 0.0 28.701368 3 TATCAAC 10875 0.0 28.61273 2 TCAACGC 10865 0.0 28.588682 4 CAACGCA 11055 0.0 28.140299 5 AACGCAG 11380 0.0 27.420113 6 TAAGGTG 1565 0.0 25.492418 5 GTAAGGT 1480 0.0 24.710135 4 ACGCAGA 12810 0.0 24.322088 7 CGCAGAG 12880 0.0 24.189903 8 AGGTAAG 1405 0.0 23.330286 2 GGTAAGG 1540 0.0 23.132204 3 GTACATG 6035 0.0 22.355703 1 TACATGG 5800 0.0 22.278616 2 GAGTACT 8940 0.0 22.25987 12-13 GCAGAGT 14105 0.0 21.853334 9 TACCCCG 135 3.800231E-4 21.108803 5 GTACTTT 9440 0.0 21.106007 14-15 ACATGGG 5985 0.0 20.713469 3 >>END_MODULE