FastQCFastQC Report
Thu 26 May 2016
SRR937993_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937993_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2196967
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91110.4147080952968342No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72880.33173006239966285No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47390.2157064716948411No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA30950.14087603500644297No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA28240.12854084745014377No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA27550.1254001539394993No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA24150.10992427287255567No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC23220.10569116422777401No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52800.043.499021
GTATCAA87100.037.2785531
ATCAACG108350.029.6957363
TCAACGC108600.029.6273754
TATCAAC109800.029.433432
CAACGCA110250.029.2270775
AACGCAG114950.028.1147526
ACGCAGA126850.025.22427
CGCAGAG128450.024.835978
GTACATG66050.023.639771
TACATGG65150.022.8330692
GTGTAGC12550.022.4479471
GCAGAGT139250.022.4312279
GAGTACT86450.021.94173412-13
AGAGTAC115450.021.53726810-11
GTATTAG7700.021.0841711
GTACTTT90100.021.03871314-15
ACATGGG66700.021.0198943
TAGCCCT13350.020.6481464
AGTACTT89150.020.6372912-13
Produced by FastQC (version 0.11.5)