##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937993_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2196967 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.90893354338049 33.0 31.0 34.0 30.0 34.0 2 32.10043937847041 34.0 31.0 34.0 30.0 34.0 3 32.17158382442704 34.0 31.0 34.0 30.0 34.0 4 35.68862572810607 37.0 35.0 37.0 35.0 37.0 5 35.71100840385859 37.0 35.0 37.0 35.0 37.0 6 35.737514946742486 37.0 36.0 37.0 35.0 37.0 7 35.67844350870996 37.0 35.0 37.0 33.0 37.0 8 35.65947508542459 37.0 35.0 37.0 33.0 37.0 9 37.334517996856576 39.0 37.0 39.0 34.0 39.0 10-11 37.25408984295167 39.0 37.0 39.0 34.0 39.0 12-13 37.15945960954352 39.0 37.0 39.0 34.0 39.0 14-15 38.421645386571576 40.0 38.0 41.0 34.0 41.0 16-17 38.34433380200977 40.0 38.0 41.0 34.0 41.0 18-19 38.28718410426738 40.0 38.0 41.0 33.5 41.0 20-21 38.18046766291893 40.0 38.0 41.0 33.5 41.0 22-23 38.03241332254877 40.0 38.0 41.0 33.0 41.0 24-25 37.98419070473066 40.0 38.0 41.0 33.0 41.0 26-27 37.9934584816249 40.0 38.0 41.0 33.0 41.0 28-29 37.90481217970047 40.0 38.0 41.0 33.0 41.0 30-31 37.71630024483754 40.0 37.5 41.0 33.0 41.0 32-33 37.490939781981254 40.0 37.0 41.0 32.0 41.0 34-35 37.282916174890204 40.0 37.0 41.0 31.5 41.0 36-37 37.055068874498346 40.0 36.5 41.0 31.0 41.0 38-39 36.811003078334814 40.0 36.0 41.0 30.0 41.0 40-41 36.59061833882803 39.0 36.0 41.0 30.0 41.0 42-43 36.30142555623275 39.0 35.0 41.0 30.0 41.0 44-45 36.07895111760896 39.0 35.0 41.0 29.0 41.0 46-47 35.939926953841365 39.0 35.0 41.0 28.5 41.0 48-49 35.684134081212875 39.0 35.0 41.0 27.0 41.0 50-51 35.04190026522929 38.5 34.5 40.5 26.5 40.5 52-53 35.114960306640924 38.5 35.0 40.0 26.5 41.0 54-55 35.25544580323692 39.0 35.0 40.5 26.0 41.0 56-57 35.04591352532833 39.0 35.0 40.5 26.0 41.0 58-59 34.78295418183341 38.0 34.5 40.0 25.5 41.0 60-61 34.41439926043495 38.0 34.0 40.0 24.0 41.0 62-63 33.998253501304305 37.5 34.0 40.0 23.0 41.0 64-65 33.5786220275498 37.0 33.5 40.0 22.0 41.0 66-67 33.14615103458541 36.0 33.0 39.0 20.5 41.0 68-69 32.66170338471174 36.0 32.5 39.0 18.5 40.5 70-71 32.17330961275249 35.0 32.0 38.5 16.5 40.0 72-73 31.688077927433593 35.0 31.0 37.5 11.5 40.0 74-75 31.160659673085668 35.0 31.0 37.0 7.5 39.0 76-77 30.621326811008085 35.0 30.5 36.5 4.5 39.0 78-79 30.054445515112427 34.0 30.0 36.0 2.0 38.5 80-81 29.516103337009614 34.0 29.0 35.5 2.0 37.0 82-83 28.967557546380988 34.0 29.0 35.0 2.0 37.0 84-85 28.472271772857763 34.0 28.5 35.0 2.0 36.5 86-87 27.972211234852413 34.0 27.0 35.0 2.0 36.0 88-89 27.406741885517626 33.0 26.0 35.0 2.0 35.5 90-91 26.79547462478954 33.0 25.0 35.0 2.0 35.0 92-93 26.188083389509266 32.5 24.5 35.0 2.0 35.0 94-95 25.58035600898876 32.0 21.5 35.0 2.0 35.0 96-97 24.740992468252823 32.0 14.5 34.0 2.0 35.0 98-99 23.667662964441433 31.0 2.0 34.0 2.0 35.0 100-101 20.776355539250247 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.44582920518179137 1101 2 0.2473146283568184 1101 3 0.3400075408399914 1101 4 0.13473674345987519 1101 5 0.2429544349823871 1101 6 0.2529540139748221 1101 7 0.2713590898073903 1101 8 0.2902614703955564 1101 9 0.3086642946662792 1101 10-11 0.20261007970038492 1101 12-13 0.21251907143722804 1101 14-15 0.2951540054710904 1101 16-17 0.5040842136694863 1101 18-19 0.34595630942210676 1101 20-21 0.447010465319984 1101 22-23 0.4098222168895589 1101 24-25 0.4309245472205774 1101 26-27 0.3540422829001031 1101 28-29 0.30977316275919264 1101 30-31 0.33534629962085205 1101 32-33 0.4546208149478659 1101 34-35 0.4610288125684221 1101 36-37 0.4753859730371346 1101 38-39 0.5634330733021571 1101 40-41 0.5659546099539625 1101 42-43 0.5996751024463762 1101 44-45 0.6691515032928805 1101 46-47 0.8254475161709252 1101 48-49 0.7232382204363716 1101 50-51 0.6170239146324121 1101 52-53 0.6178592188742229 1101 54-55 0.6166075270685027 1101 56-57 0.43496073734077356 1101 58-59 0.49465885684184485 1101 60-61 0.45391010818098465 1101 62-63 0.4369576648102367 1101 64-65 0.2302219093770148 1101 66-67 0.3633768133996895 1101 68-69 0.3657378250129071 1101 70-71 0.4867426305376128 1101 72-73 0.6847963590684678 1101 74-75 0.5849416390327065 1101 76-77 0.525560167047928 1101 78-79 0.45974143770135356 1101 80-81 0.603779065018994 1101 82-83 0.41221870729252075 1101 84-85 0.4970997738929519 1101 86-87 0.7340053154339792 1101 88-89 0.6226682504087009 1101 90-91 0.7612918956855914 1101 92-93 0.6942526137788398 1101 94-95 0.7464010584603251 1101 96-97 0.7650664303505792 1101 98-99 0.89610054534635 1101 100-101 0.7423570076889057 1102 1 0.36972197875129 1102 2 0.27784119134365426 1102 3 0.16849342146673507 1102 4 0.2282399733999938 1102 5 0.20843239862695384 1102 6 0.20347680476882601 1102 7 0.23638170909605094 1102 8 0.2543160784342078 1102 9 0.2459367958427805 1102 10-11 0.3330814928581347 1102 12-13 0.2737307589480338 1102 14-15 0.27814331111949286 1102 16-17 0.33383627520060344 1102 18-19 0.2522388133815312 1102 20-21 0.3095849961766106 1102 22-23 0.4014783204917194 1102 24-25 0.30138162049819783 1102 26-27 0.29162705322626437 1102 28-29 0.4025071612188853 1102 30-31 0.3657140699312862 1102 32-33 0.3497499247195961 1102 34-35 0.3274973466554485 1102 36-37 0.29615827404703055 1102 38-39 0.31965511122933066 1102 40-41 0.3605434548031212 1102 42-43 0.4425565677583876 1102 44-45 0.3045732261310121 1102 46-47 0.3229951285485271 1102 48-49 0.08843826222635442 1102 50-51 0.2706679638595304 1102 52-53 0.2850519585124758 1102 54-55 0.2235196081632509 1102 56-57 0.14156041060230962 1102 58-59 0.21102809349979879 1102 60-61 0.25390714959836913 1102 62-63 0.2459432500409946 1102 64-65 0.18979380247233024 1102 66-67 0.09921514165257861 1102 68-69 0.10946193163652396 1102 70-71 0.2287516838004322 1102 72-73 0.25661086703306424 1102 74-75 0.17743871974376546 1102 76-77 0.26065145319001815 1102 78-79 0.07981736232776626 1102 80-81 0.2390043194802125 1102 82-83 0.4422312165496507 1102 84-85 0.42439653544690614 1102 86-87 0.3743711071922924 1102 88-89 0.4434904775002586 1102 90-91 0.5962069150227016 1102 92-93 0.5510796356233456 1102 94-95 0.7256059208908532 1102 96-97 0.6363112262499833 1102 98-99 0.9190106072084632 1102 100-101 1.145722302960074 1103 1 0.2960038620936949 1103 2 0.2775328541477151 1103 3 0.23462402477312594 1103 4 0.07939170351425417 1103 5 0.06630910405409196 1103 6 0.048668020853412486 1103 7 0.17530608923080848 1103 8 0.12042854425848759 1103 9 0.18767248570527073 1103 10-11 0.1556962460204332 1103 12-13 0.015510113119319158 1103 14-15 0.23508526445913702 1103 16-17 0.22231662290285925 1103 18-19 0.2509063802047109 1103 20-21 0.254990839132887 1103 22-23 0.3166493166425468 1103 24-25 0.24299202738237824 1103 26-27 0.035971181720242384 1103 28-29 0.2738566570640586 1103 30-31 0.2973068767402367 1103 32-33 0.2777723345863947 1103 34-35 0.20370500067290465 1103 36-37 0.3844548957730467 1103 38-39 0.3420387794343256 1103 40-41 0.4164646709548805 1103 42-43 0.31032861319614113 1103 44-45 0.33366406804606186 1103 46-47 0.3140147237467943 1103 48-49 0.3772259394866566 1103 50-51 0.39149046508548935 1103 52-53 0.4993725082797127 1103 54-55 0.46900774154764235 1103 56-57 0.2868738867515219 1103 58-59 0.3580283554856507 1103 60-61 0.15248671604537378 1103 62-63 0.3244442879080296 1103 64-65 0.190306018402417 1103 66-67 0.3038234056268436 1103 68-69 0.23631480978623642 1103 70-71 0.3941215204959079 1103 72-73 0.3967652371586148 1103 74-75 0.17658357104999922 1103 76-77 0.3304347438666788 1103 78-79 0.48629983278718214 1103 80-81 0.522090445649912 1103 82-83 0.5325642744619579 1103 84-85 0.5554720881362947 1103 86-87 0.44719712843329873 1103 88-89 0.4931549340811401 1103 90-91 0.7289067799992246 1103 92-93 0.8016801878089161 1103 94-95 0.854699785719589 1103 96-97 0.7442530922610153 1103 98-99 0.6312772550658892 1103 100-101 0.6769768793155144 1104 1 0.3527759708513578 1104 2 0.26184192654342553 1104 3 0.25147519406112195 1104 4 0.24991882981755964 1104 5 0.21692404266541843 1104 6 0.26270200131338584 1104 7 0.2091342346112981 1104 8 0.24404235523692108 1104 9 0.24113112093285594 1104 10-11 0.29664488823261337 1104 12-13 0.2550616265878034 1104 14-15 0.34045582650840345 1104 16-17 0.35686437058237175 1104 18-19 0.42046712932322095 1104 20-21 0.36019457041964387 1104 22-23 0.4203891237668316 1104 24-25 0.5721976142143177 1104 26-27 0.4758953820686358 1104 28-29 0.6213662924347076 1104 30-31 0.6018339230994059 1104 32-33 0.5128242561906617 1104 34-35 0.4980632597423522 1104 36-37 0.5495076378564789 1104 38-39 0.4035381104282436 1104 40-41 0.4369032275847786 1104 42-43 0.53473050492655 1104 44-45 0.4918983127423999 1104 46-47 0.46211152766489505 1104 48-49 0.4714123186910868 1104 50-51 0.396287638429591 1104 52-53 0.44763687928777074 1104 54-55 0.49381324591090703 1104 56-57 0.5228885039033173 1104 58-59 0.5426443712388433 1104 60-61 0.6714134872284632 1104 62-63 0.697619037346314 1104 64-65 0.5714175362467842 1104 66-67 0.7442573549506761 1104 68-69 0.8554530121374384 1104 70-71 0.842346550580146 1104 72-73 0.8914138196230859 1104 74-75 0.750881535420163 1104 76-77 0.613950055270081 1104 78-79 0.6733847544840188 1104 80-81 0.9009788857296854 1104 82-83 0.935579956132873 1104 84-85 0.789991960562304 1104 86-87 0.8241833191379015 1104 88-89 1.0273793288199755 1104 90-91 1.0419150563197768 1104 92-93 1.0535839098389879 1104 94-95 1.0649055997611434 1104 96-97 0.968876197795673 1104 98-99 1.1159140983669928 1104 100-101 1.2488217161874289 1105 1 0.3282865048240957 1105 2 0.32405528109156023 1105 3 0.3072508126764717 1105 4 0.24566134256703975 1105 5 0.19346190442901445 1105 6 0.27816144226957107 1105 7 0.2502241767037674 1105 8 0.28020207479585224 1105 9 0.3122057224421937 1105 10-11 0.45439853875257796 1105 12-13 0.33066510737879184 1105 14-15 0.43922192381967307 1105 16-17 0.5719900983884472 1105 18-19 0.38042016463509043 1105 20-21 0.5433707208841483 1105 22-23 0.35534477395082575 1105 24-25 0.5376327685551701 1105 26-27 0.4063138329233027 1105 28-29 0.4698298965842653 1105 30-31 0.3881491483112143 1105 32-33 0.42496336421815073 1105 34-35 0.5689631402506237 1105 36-37 0.5034353738843436 1105 38-39 0.5808668521517788 1105 40-41 0.5607740782581487 1105 42-43 0.5331520730257395 1105 44-45 0.5044901106016937 1105 46-47 0.5590763346556429 1105 48-49 0.5253999194428971 1105 50-51 0.5375004398926535 1105 52-53 0.5007671189368423 1105 54-55 0.38418770352303966 1105 56-57 0.5644717059647917 1105 58-59 0.5148684863819639 1105 60-61 0.5257678832246953 1105 62-63 0.4849819942754152 1105 64-65 0.43870921017698805 1105 66-67 0.4894830342627614 1105 68-69 0.537019431474171 1105 70-71 0.5179728520733207 1105 72-73 0.7282073683373547 1105 74-75 0.6318890060305868 1105 76-77 0.7108116540504419 1105 78-79 0.8350284234824095 1105 80-81 0.7589366547524854 1105 82-83 0.6987868432306179 1105 84-85 0.6354197377942157 1105 86-87 0.6223191530285455 1105 88-89 0.7337717799218701 1105 90-91 0.8643004615298686 1105 92-93 0.878929072959469 1105 94-95 0.8950956058783603 1105 96-97 0.9325744431372378 1105 98-99 1.288522599712465 1105 100-101 1.349392669553957 1106 1 -0.06718989837119338 1106 2 -0.04142623840292714 1106 3 -0.023320466181928623 1106 4 0.04832637799470518 1106 5 -0.017138727457115976 1106 6 0.03923915746327822 1106 7 0.10084369716278019 1106 8 0.019945517895180842 1106 9 0.11982862182267695 1106 10-11 -0.36523239986419753 1106 12-13 -0.22323243834330242 1106 14-15 -0.24377155946810802 1106 16-17 -0.021059308677301658 1106 18-19 0.033429197762735896 1106 20-21 -0.10451312521262679 1106 22-23 -0.20276212578826147 1106 24-25 -0.05822867093338857 1106 26-27 -0.013586522899075248 1106 28-29 -0.2756137375647896 1106 30-31 -0.011618338302938014 1106 32-33 -0.017561351680399184 1106 34-35 -0.42634215287777266 1106 36-37 -0.3496031822991128 1106 38-39 -0.2052585095443007 1106 40-41 0.04447476238927095 1106 42-43 -0.15411375195045451 1106 44-45 0.09167760673742009 1106 46-47 0.284014969979431 1106 48-49 0.18111877493758044 1106 50-51 0.11009836833247988 1106 52-53 0.11637210199585724 1106 54-55 -0.15114722086084242 1106 56-57 -0.09923151313029166 1106 58-59 0.22796273448692972 1106 60-61 -0.0435365242120227 1106 62-63 -0.02565789863781731 1106 64-65 -0.16670104437049105 1106 66-67 -0.24610959857552928 1106 68-69 -0.4644650295059236 1106 70-71 -0.09596063912849928 1106 72-73 0.05478472647211774 1106 74-75 0.1354820082935042 1106 76-77 0.2547911533023175 1106 78-79 0.2537144310996666 1106 80-81 0.17976520926657358 1106 82-83 0.19041991647253198 1106 84-85 0.15198317681287676 1106 86-87 0.13991048901637626 1106 88-89 -0.04935374561943817 1106 90-91 -0.0637680041368256 1106 92-93 0.01756783595888578 1106 94-95 0.23261117890386984 1106 96-97 0.34855487908921035 1106 98-99 0.0905940826045466 1106 100-101 0.010014323092395472 1107 1 0.28936126063467427 1107 2 0.2596088055541159 1107 3 0.09595808308343123 1107 4 0.10878347804641919 1107 5 0.12434250334019481 1107 6 0.2224208339150806 1107 7 0.12859139284008592 1107 8 0.15012339943930897 1107 9 0.20846120023048798 1107 10-11 0.15245147063743048 1107 12-13 0.052144105476855884 1107 14-15 0.2313143773189239 1107 16-17 0.20331517798349097 1107 18-19 0.27588082980118855 1107 20-21 0.10846846302087698 1107 22-23 0.21741809430117343 1107 24-25 0.342708078070352 1107 26-27 0.3312240929558783 1107 28-29 0.2423981947200673 1107 30-31 0.2179586511717062 1107 32-33 0.2647328183071309 1107 34-35 0.26643212236330527 1107 36-37 0.21965806553345146 1107 38-39 0.24071871720713744 1107 40-41 0.21845483342021055 1107 42-43 0.30527693341444007 1107 44-45 0.2141496012313837 1107 46-47 0.063450601044444 1107 48-49 -0.018865729823161814 1107 50-51 -0.14618366783702896 1107 52-53 -0.1793781147473581 1107 54-55 -0.1093043711057291 1107 56-57 -0.0029114898589170934 1107 58-59 -0.18736728559034077 1107 60-61 -0.35680909972992225 1107 62-63 -0.22924176685793896 1107 64-65 -0.1980289008180378 1107 66-67 -0.16106706747098087 1107 68-69 -0.09686899704767882 1107 70-71 0.009312305233130758 1107 72-73 0.008632203885195366 1107 74-75 -0.07837320723291086 1107 76-77 0.011092962537858853 1107 78-79 0.09878504376051467 1107 80-81 0.1533188284981648 1107 82-83 0.09506886850003227 1107 84-85 0.062245588214327796 1107 86-87 0.2798210919427895 1107 88-89 0.2324517064232694 1107 90-91 0.3410929379737233 1107 92-93 0.38389458967774104 1107 94-95 0.246290052903813 1107 96-97 0.22638442385239088 1107 98-99 0.13763439684419865 1107 100-101 0.28341004298410155 1108 1 0.3028874133250703 1108 2 0.2876950108316052 1108 3 0.27386492879664104 1108 4 0.2670809707687454 1108 5 0.27423451197950044 1108 6 0.32484923094766316 1108 7 0.2730237339049708 1108 8 0.28590234581924534 1108 9 0.2959576787730711 1108 10-11 0.27624149787003915 1108 12-13 0.23320242081162235 1108 14-15 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-0.8308762921590187 2316 58-59 -1.01780857953743 2316 60-61 -0.8700311233540106 2316 62-63 -0.9775843039674612 2316 64-65 -0.7910525370899038 2316 66-67 -0.9139981496644936 2316 68-69 -0.8672224533803572 2316 70-71 -0.9106595700224887 2316 72-73 -0.929708732063137 2316 74-75 -0.792386674390837 2316 76-77 -0.7832771284672724 2316 78-79 -0.7488381020687243 2316 80-81 -0.6692116849350533 2316 82-83 -0.7809358166368447 2316 84-85 -0.9060760467800932 2316 86-87 -0.8869054732357817 2316 88-89 -0.979612346985899 2316 90-91 -0.9844373161410012 2316 92-93 -1.0111060455655547 2316 94-95 -1.0818999561984448 2316 96-97 -1.0483509059433054 2316 98-99 -0.8709930773414314 2316 100-101 -1.0374733628946018 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6218.0 3 1619.0 4 1628.0 5 1841.0 6 2032.0 7 2556.0 8 3186.0 9 4087.0 10 5136.0 11 6276.0 12 8156.0 13 10125.0 14 11971.0 15 14191.0 16 17347.0 17 22294.0 18 20247.0 19 16633.0 20 16239.0 21 16376.0 22 17646.0 23 19373.0 24 21315.0 25 24023.0 26 26849.0 27 31484.0 28 37113.0 29 43286.0 30 52352.0 31 64616.0 32 79605.0 33 103767.0 34 142412.0 35 208594.0 36 315183.0 37 438083.0 38 339864.0 39 43240.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.454576309796025 18.591677374173265 14.579850248906645 23.373896067124058 2 15.229679082285545 19.62170236214018 41.004786214245705 24.143832341328572 3 19.231546971139178 23.686734586990084 29.896343324027306 27.18537511784343 4 12.553102236408629 16.57359114819253 37.43052357768902 33.442783037709816 5 13.44405901016529 37.34089827192325 34.70420892714381 14.510833790767647 6 32.229589828086496 36.45333620098861 17.133207448127035 14.183866522797858 7 28.35646993608112 32.83325111743027 20.60512726611853 18.205151680370076 8 26.104685980767872 35.247984224464375 19.59613900141406 19.05119079335369 9 26.03957036844754 16.90158868949625 20.406724548975674 36.65211639308053 10-11 24.873936488588004 26.4916035096446 27.370517855694978 21.26394214607242 12-13 25.97721628579891 23.908227199198013 27.661935248684237 22.452621266318832 14-15 22.443037686439713 25.441534783103727 26.13983832403507 25.975589206421496 16-17 21.893698904652382 28.512149021929723 27.16626056065402 22.42789151276387 18-19 21.925144284371378 26.913111477894613 29.561034839574724 21.60070939815928 20-21 22.265201788227298 26.139762594583072 29.950985226330918 21.644050390858713 22-23 22.03826071431174 26.074687734563444 29.873203653906415 22.013847897218398 24-25 21.9150569476082 26.250159453302963 29.893462414578586 21.94132118451025 26-27 21.47784850989249 26.62822339667948 29.703108065730845 22.190820027697193 28-29 21.620082864064084 27.11906750257968 29.478436464014163 21.78241316934207 30-31 21.809688609684418 26.52181062236158 29.453563834923596 22.2149369330304 32-33 21.464264297913402 26.613310048457777 29.44341085165389 22.479014801974934 34-35 22.29898633414078 26.76735607848782 29.197302721419987 21.736354865951412 36-37 22.324491643847345 26.228485616996156 29.288096422331417 22.15892631682508 38-39 21.799365548023662 26.704585945292692 29.225841543649484 22.270206963034166 40-41 22.30244886045134 26.52917735465512 29.203538614053453 21.964835170840086 42-43 21.930535968557994 26.498957443000887 29.046812835632092 22.52369375280903 44-45 22.05094794983057 26.780521717277256 29.15318485010314 22.01534548278903 46-47 22.52551685358727 27.07255357486367 28.392179270764917 22.009750300784145 48-49 22.09972786507717 27.13941439769944 28.393411393606723 22.367446343616667 50-51 22.209681307741672 27.33150410006709 28.445639650759563 22.01317494143168 52-53 22.587055231008293 27.041517615472277 28.308321871359087 22.063105282160343 54-55 22.084118419306364 27.29988929690216 28.435057028144268 22.180935255647213 56-57 22.238931276161964 27.35360911012223 28.242171102533494 22.165288511182318 58-59 22.268784275143112 26.989605829167374 28.239087099468396 22.502522796221122 60-61 21.90045034605755 27.174111498781368 28.613889380875477 22.31154877428561 62-63 21.9757216401915 27.195309498097902 28.761921108078038 22.067047753632558 64-65 21.73726182216176 27.277383508432628 28.553136216871817 22.432218452533796 66-67 21.686640581075885 27.291286820858456 28.40060729804722 22.621465300018436 68-69 22.26386060915774 26.91192923472297 28.58513833578224 22.23907182033705 70-71 22.10349707795135 27.0889209867481 28.158676758670747 22.648905176629803 72-73 22.04549335312559 27.168675110250007 28.265551682607477 22.520279854016927 74-75 22.052495295439137 27.59925564809111 27.972478831183555 22.3757702252862 76-77 22.17750664644336 27.223340301314536 28.111945705745413 22.487207346496692 78-79 22.2907464118884 27.393610846437884 27.99701269536185 22.31863004631187 80-81 22.509878404404656 27.505657078068246 27.663993804927607 22.320470712599494 82-83 22.387523907472335 27.595858663530752 27.815800579925508 22.2008168490714 84-85 22.42574945944045 27.72108197042976 27.721582751990436 22.131585818139353 86-87 22.59142352762303 27.71232008681901 27.465091676173113 22.231164709384846 88-89 22.47158240334602 27.762327004144954 27.32112424964453 22.444966342864493 90-91 22.47775757763443 27.7505642580121 27.396958016289254 22.374720148064217 92-93 22.672485448302915 27.33094089098026 27.651423137420338 22.34515052329649 94-95 22.439248988915818 27.228967843640532 27.773510767098713 22.558272400344933 96-97 22.751978826938824 26.891121940912516 27.72614091633963 22.63075831580903 98-99 22.634106117442272 27.199620805864942 27.23479461342164 22.931478463271144 100-101 23.010230705521327 26.574506651201034 27.600928393724296 22.814334249553344 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 574.0 1 419.0 2 375.0 3 498.5 4 421.5 5 381.0 6 738.0 7 1669.0 8 2747.0 9 3164.0 10 2626.0 11 2017.0 12 1962.0 13 2095.0 14 2270.0 15 2415.0 16 2602.0 17 2888.5 18 3163.0 19 3505.5 20 4043.5 21 4798.5 22 5945.5 23 7536.0 24 9498.0 25 12008.0 26 15212.0 27 18862.5 28 21908.5 29 24741.5 30 27784.0 31 32412.0 32 39645.5 33 43106.0 34 43341.5 35 45811.0 36 49922.5 37 54548.0 38 58410.5 39 61067.0 40 64016.5 41 67830.5 42 69849.5 43 71500.5 44 73813.0 45 77759.5 46 79624.5 47 81157.5 48 83548.0 49 80907.5 50 77646.5 51 75185.0 52 73272.0 53 72938.0 54 73045.5 55 69092.5 56 63176.0 57 58192.5 58 53740.0 59 47270.0 60 37263.5 61 30395.5 62 26151.0 63 19574.5 64 13547.5 65 10309.0 66 7633.0 67 5618.0 68 4273.0 69 3238.0 70 2406.5 71 1696.5 72 1195.0 73 930.0 74 724.0 75 507.0 76 372.0 77 247.5 78 150.0 79 97.0 80 68.0 81 48.5 82 35.0 83 26.0 84 15.0 85 7.5 86 4.5 87 4.0 88 4.0 89 3.0 90 3.0 91 2.5 92 2.5 93 2.0 94 1.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4918599141452739 2 0.04902212914440681 3 0.0572152426504358 4 0.06545387345372052 5 0.07974630479201554 6 0.108103580982327 7 0.06955043020673501 8 0.05289109941114272 9 0.051525580493471224 10-11 0.07487595398565386 12-13 0.11001530746706709 14-15 0.05539455076020714 16-17 0.0599462804857788 18-19 0.05930903832419877 20-21 0.07321457263582021 22-23 0.06367869886074756 24-25 0.08953252370199462 26-27 0.06543111480509266 28-29 0.06570421858862695 30-31 0.06932284372045643 32-33 0.07057456939498864 34-35 0.0714166393942194 36-37 0.06640973669609057 38-39 0.04089729158426139 40-41 0.04396970914902226 42-43 0.042057982664282165 44-45 0.02230347565530115 46-47 0.028425552136195038 48-49 0.028675897271101477 50-51 0.02733313700205784 52-53 0.026126928624781345 54-55 0.025102789436527724 56-57 0.025171065382411296 58-59 0.02255382079020759 60-61 0.029859346999750108 62-63 0.020164162684282467 64-65 0.0227131313306026 66-67 0.016022088634012253 68-69 0.01863933322621596 70-71 0.04119315401642355 72-73 0.03741521834419907 74-75 0.03158900429546734 76-77 0.022371751601184726 78-79 0.015384846472432222 80-81 0.01741036620031161 82-83 0.02175726808823255 84-85 0.01822967755091451 86-87 0.009695184315467642 88-89 0.04208074131291002 90-91 0.05293661670839844 92-93 0.03393314510413675 94-95 0.05373316941037348 96-97 0.044106261040789414 98-99 0.033250385645300994 100-101 0.16916503525087087 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2196967.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.7987167693561 #Duplication Level Percentage of deduplicated Percentage of total 1 78.10563563767106 45.92511147949946 2 12.882972501922529 15.150045025758912 3 4.05972025760366 7.161190247689649 4 1.717899732971473 4.040411993485684 5 0.9178769809655688 2.6984994316453066 6 0.5415543830666121 1.9105621671082262 7 0.3588010972252419 1.476793086459684 8 0.2483338118433309 1.1681367573464465 9 0.17661551271106662 0.9346288958075356 >10 0.8984251880754145 9.528877015883722 >50 0.05775432081046599 2.2789733869452404 >100 0.028976575451373592 3.289111096793826 >500 0.0026393712741838863 1.057722343061746 >1k 0.002639371274183752 2.6314112578219175 >5k 1.552571337755148E-4 0.7485258146924826 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9111 0.4147080952968342 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7288 0.33173006239966285 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4739 0.2157064716948411 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3095 0.14087603500644297 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2824 0.12854084745014377 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2755 0.1254001539394993 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2415 0.10992427287255567 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2322 0.10569116422777401 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.551729725571663E-5 2 4.551729725571663E-5 0.0 0.0 0.0 4.551729725571663E-5 3 4.551729725571663E-5 0.0 0.0 0.0 4.551729725571663E-5 4 4.551729725571663E-5 0.0 0.0 0.0 4.551729725571663E-5 5 4.551729725571663E-5 0.0 0.0 0.0 9.103459451143326E-5 6 4.551729725571663E-5 0.0 0.0 0.0 9.103459451143326E-5 7 4.551729725571663E-5 0.0 0.0 0.0 9.103459451143326E-5 8 4.551729725571663E-5 0.0 0.0 0.0 9.103459451143326E-5 9 4.551729725571663E-5 0.0 0.0 0.0 9.103459451143326E-5 10-11 4.551729725571663E-5 0.0 0.0 0.0 9.103459451143326E-5 12-13 4.551729725571663E-5 0.0 0.0 0.0 2.2758648627858315E-4 14-15 4.551729725571663E-5 0.0 0.0 0.0 2.731037835342998E-4 16-17 9.103459451143326E-5 0.0 0.0 0.0 3.1862108079001644E-4 18-19 1.365518917671499E-4 0.0 0.0 1.1379324313929158E-4 3.64138378045733E-4 20-21 1.365518917671499E-4 0.0 0.0 1.365518917671499E-4 4.3241432392930796E-4 22-23 1.365518917671499E-4 0.0 0.0 1.820691890228665E-4 4.551729725571663E-4 24-25 1.365518917671499E-4 0.0 0.0 3.64138378045733E-4 4.551729725571663E-4 26-27 1.365518917671499E-4 0.0 0.0 0.0010924151341371992 4.551729725571663E-4 28-29 1.365518917671499E-4 0.0 0.0 0.0032089694565280223 4.551729725571663E-4 30-31 1.365518917671499E-4 0.0 0.0 0.010560012963326258 4.551729725571663E-4 32-33 1.365518917671499E-4 0.0 0.0 0.028402793487567177 4.551729725571663E-4 34-35 1.365518917671499E-4 0.0 0.0 0.05398351454527992 4.551729725571663E-4 36-37 1.365518917671499E-4 0.0 2.2758648627858314E-5 0.09731598153272217 4.551729725571663E-4 38-39 1.365518917671499E-4 0.0 4.551729725571663E-5 0.16226916471662978 4.551729725571663E-4 40-41 1.365518917671499E-4 0.0 4.551729725571663E-5 0.2264713124958181 4.551729725571663E-4 42-43 1.820691890228665E-4 0.0 4.551729725571663E-5 0.2935182913534887 4.551729725571663E-4 44-45 3.1862108079001644E-4 0.0 4.551729725571663E-5 0.377634256682053 4.779316211850246E-4 46-47 3.1862108079001644E-4 0.0 4.551729725571663E-5 0.4718778206500143 5.234489184407412E-4 48-49 3.1862108079001644E-4 0.0 4.551729725571663E-5 0.5705820797490357 5.462075670685996E-4 50-51 3.1862108079001644E-4 0.0 4.551729725571663E-5 0.6789132472176413 5.917248643243162E-4 52-53 3.1862108079001644E-4 0.0 4.551729725571663E-5 0.7792788876664966 6.372421615800329E-4 54-55 3.1862108079001644E-4 0.0 4.551729725571663E-5 0.8841962578409235 6.372421615800329E-4 56-57 3.1862108079001644E-4 0.0 4.551729725571663E-5 1.011143999887117 6.600008102078912E-4 58-59 3.1862108079001644E-4 0.0 4.551729725571663E-5 1.1442820943600882 6.827594588357495E-4 60-61 3.1862108079001644E-4 0.0 4.551729725571663E-5 1.2841795074755333 6.827594588357495E-4 62-63 3.1862108079001644E-4 0.0 4.551729725571663E-5 1.425101059779232 7.737940533471827E-4 64-65 3.1862108079001644E-4 0.0 4.551729725571663E-5 1.5670695099198122 7.737940533471827E-4 66-67 3.1862108079001644E-4 0.0 4.551729725571663E-5 1.729338674636442 7.737940533471827E-4 68-69 3.1862108079001644E-4 0.0 4.551729725571663E-5 1.910065103390265 7.737940533471827E-4 70-71 3.1862108079001644E-4 0.0 4.551729725571663E-5 2.0964356770037966 7.737940533471827E-4 72-73 3.1862108079001644E-4 0.0 4.551729725571663E-5 2.285560047101299 7.965527019750411E-4 74-75 3.1862108079001644E-4 0.0 4.551729725571663E-5 2.473409932875642 8.193113506028993E-4 76-77 3.1862108079001644E-4 0.0 4.551729725571663E-5 2.674619145394537 8.193113506028993E-4 78-79 3.1862108079001644E-4 0.0 4.551729725571663E-5 2.9002483878911245 8.64828647858616E-4 80-81 3.64138378045733E-4 0.0 4.551729725571663E-5 3.1389183360514745 8.875872964864743E-4 82-83 3.64138378045733E-4 0.0 4.551729725571663E-5 3.378544147454195 9.103459451143326E-4 84-85 3.64138378045733E-4 0.0 4.551729725571663E-5 3.6268182453355013 9.103459451143326E-4 86-87 3.64138378045733E-4 0.0 4.551729725571663E-5 3.879052348078055 9.558632423700492E-4 88-89 3.64138378045733E-4 0.0 4.551729725571663E-5 4.14997130134408 0.0010013805396257658 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5280 0.0 43.49902 1 GTATCAA 8710 0.0 37.278553 1 ATCAACG 10835 0.0 29.695736 3 TCAACGC 10860 0.0 29.627375 4 TATCAAC 10980 0.0 29.43343 2 CAACGCA 11025 0.0 29.227077 5 AACGCAG 11495 0.0 28.114752 6 ACGCAGA 12685 0.0 25.2242 7 CGCAGAG 12845 0.0 24.83597 8 GTACATG 6605 0.0 23.63977 1 TACATGG 6515 0.0 22.833069 2 GTGTAGC 1255 0.0 22.447947 1 GCAGAGT 13925 0.0 22.431227 9 GAGTACT 8645 0.0 21.941734 12-13 AGAGTAC 11545 0.0 21.537268 10-11 GTATTAG 770 0.0 21.084171 1 GTACTTT 9010 0.0 21.038713 14-15 ACATGGG 6670 0.0 21.019894 3 TAGCCCT 1335 0.0 20.648146 4 AGTACTT 8915 0.0 20.63729 12-13 >>END_MODULE