FastQCFastQC Report
Thu 26 May 2016
SRR937993_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937993_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2196967
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85970.3913122045073959No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72820.3314569586161285No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47600.21666233493721115No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA30820.14028431014211865No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA28680.1305436085293953No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA27520.12526360204773218No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC26740.12171325286178628No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA25250.11493117557068448No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG22400.10195874585280525No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55400.042.192831
GTATCAA85250.033.716641
ATCAACG104350.027.4023933
TATCAAC105650.027.1163432
TCAACGC105450.027.1146974
CAACGCA107600.026.5729055
AACGCAG109750.026.0523416
ACGCAGA122400.023.243437
CGCAGAG123450.023.007268
GTACATG60850.022.5642321
TACATGG59400.022.4751822
GAGTACT81450.022.39261612-13
ACATGGG59800.021.4460373
TAAGGTG15350.021.3498845
GTACTTT85950.020.97155414-15
TACACCG2751.2623786E-920.725425
GCAGAGT136450.020.7108659
GTAAGGT15750.020.5061054
AGAGTAC110100.020.40485810-11
GGACCGA4850.019.5858786
Produced by FastQC (version 0.11.5)