##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937993_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2196967 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.47466256889612 34.0 31.0 34.0 31.0 34.0 2 32.69436500411704 34.0 31.0 34.0 31.0 34.0 3 32.75607052814175 34.0 31.0 34.0 31.0 34.0 4 36.22036243603113 37.0 35.0 37.0 35.0 37.0 5 36.18045423531623 37.0 35.0 37.0 35.0 37.0 6 36.221915031040524 37.0 37.0 37.0 35.0 37.0 7 36.18648254616478 37.0 37.0 37.0 35.0 37.0 8 36.17537541528844 37.0 37.0 37.0 35.0 37.0 9 37.88697190262758 39.0 38.0 39.0 35.0 39.0 10-11 37.86944205352197 39.0 38.0 39.0 35.0 39.0 12-13 37.823858073425775 39.0 38.0 39.0 35.0 39.0 14-15 39.14068804856878 40.0 38.5 41.0 36.0 41.0 16-17 39.09118958090859 40.0 38.0 41.0 36.0 41.0 18-19 39.116412080836895 40.0 38.5 41.0 36.0 41.0 20-21 39.039616890012454 40.0 39.0 41.0 35.5 41.0 22-23 38.89361697285394 40.0 38.0 41.0 35.0 41.0 24-25 38.854617069805784 40.0 38.0 41.0 35.0 41.0 26-27 38.81820755614444 40.0 38.0 41.0 35.0 41.0 28-29 38.759567849676394 40.0 38.0 41.0 35.0 41.0 30-31 38.564990734954144 40.0 38.0 41.0 34.5 41.0 32-33 38.389695657695356 40.0 38.0 41.0 34.0 41.0 34-35 38.189164652905575 40.0 38.0 41.0 34.0 41.0 36-37 38.05096298669939 40.0 38.0 41.0 33.5 41.0 38-39 38.026323790935415 40.0 38.0 41.0 33.0 41.0 40-41 37.97053050865125 40.0 38.0 41.0 33.0 41.0 42-43 37.81623142268409 40.0 37.5 41.0 33.0 41.0 44-45 37.61862422148353 40.0 37.0 41.0 33.0 41.0 46-47 37.31246236288483 40.0 37.0 41.0 32.5 41.0 48-49 36.97241515234411 40.0 36.5 41.0 31.0 41.0 50-51 36.678617612371966 40.0 36.0 41.0 31.0 41.0 52-53 36.26292634345441 39.0 35.0 41.0 30.0 41.0 54-55 36.04021271143354 39.0 35.0 41.0 29.0 41.0 56-57 35.79305651837283 39.0 35.0 40.0 29.0 41.0 58-59 35.452704569526986 38.5 35.0 40.0 28.0 41.0 60-61 35.085421856586834 38.0 34.0 40.0 27.5 41.0 62-63 34.72767843121904 37.5 34.0 40.0 26.5 41.0 64-65 34.33583003294997 37.0 34.0 40.0 26.0 41.0 66-67 33.91514710962886 36.5 33.5 39.0 26.0 41.0 68-69 33.453707770758506 36.0 33.0 39.0 25.0 40.0 70-71 32.92721875203405 35.0 32.5 38.5 23.5 40.0 72-73 32.5007828975128 35.0 32.0 37.5 22.5 40.0 74-75 32.056376814034984 35.0 32.0 37.0 21.5 39.0 76-77 30.75124956360291 34.0 30.0 35.5 20.5 38.5 78-79 31.482834516858922 34.5 31.5 36.0 21.0 39.0 80-81 31.316321319346173 35.0 31.0 36.0 20.0 37.5 82-83 30.930953446273886 34.5 31.0 35.0 19.0 37.0 84-85 30.535298208848836 34.0 31.0 35.0 17.5 37.0 86-87 30.09173760916755 34.0 30.5 35.0 11.5 36.0 88-89 29.59782122353226 34.0 30.0 35.0 7.0 36.0 90-91 29.234031735569992 34.0 29.5 35.0 2.0 35.0 92-93 28.808123654110418 34.0 29.0 35.0 2.0 35.0 94-95 28.361390271223918 34.0 29.0 35.0 2.0 35.0 96-97 27.749167602426436 33.5 28.0 35.0 2.0 35.0 98-99 27.02082848763773 33.0 26.5 35.0 2.0 35.0 100-101 24.49299648105775 31.0 20.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.07165267961656241 1101 2 0.07734676262794693 1101 3 0.08990381389204316 1101 4 0.08018347519443125 1101 5 0.0970848778379505 1101 6 0.13397426374505983 1101 7 0.15015506326754036 1101 8 0.12304411215160371 1101 9 0.052453529613011085 1101 10-11 0.15530358688251056 1101 12-13 0.16148368404041946 1101 14-15 0.10397387808120584 1101 16-17 0.1731987451073067 1101 18-19 0.23553175014838956 1101 20-21 0.18444259103052474 1101 22-23 0.1966580536576359 1101 24-25 0.13298524584525495 1101 26-27 0.11166322783496696 1101 28-29 0.17002007113412532 1101 30-31 0.20986901633592225 1101 32-33 0.15059854344054457 1101 34-35 0.13742279898547594 1101 36-37 0.1523573189629488 1101 38-39 0.21117147787681745 1101 40-41 0.24949029339869355 1101 42-43 0.300253056467767 1101 44-45 0.30806725312345407 1101 46-47 0.36300237553456327 1101 48-49 0.3196426259836045 1101 50-51 0.15690568714904884 1101 52-53 0.19708672176856368 1101 54-55 0.17044119999874852 1101 56-57 0.2420948399906635 1101 58-59 0.2661617176792248 1101 60-61 0.25065672297683506 1101 62-63 0.19319367347259941 1101 64-65 0.27931822168589093 1101 66-67 0.14793200058608846 1101 68-69 0.30181548394178037 1101 70-71 0.28332193630772906 1101 72-73 0.19976898116723163 1101 74-75 0.2504479447169743 1101 76-77 0.13116458021740485 1101 78-79 0.23173154718393718 1101 80-81 0.4325298019173225 1101 82-83 0.4092923570170619 1101 84-85 0.38730851250799603 1101 86-87 0.41676187233942485 1101 88-89 0.3384440419751087 1101 90-91 0.4532491022874403 1101 92-93 0.38981564902156407 1101 94-95 0.2010178054959546 1101 96-97 0.37478486813899536 1101 98-99 0.6040239691345128 1101 100-101 0.7694424758271019 1102 1 0.17962846361783846 1102 2 0.16951262076945994 1102 3 0.13293686033531316 1102 4 0.11466067053207496 1102 5 0.09846524154021097 1102 6 0.11455532937104351 1102 7 0.11780018867908382 1102 8 0.0792256544792096 1102 9 0.17119115788374728 1102 10-11 0.18959841333852268 1102 12-13 0.17687293643432866 1102 14-15 0.2130050439754072 1102 16-17 0.15369914312912414 1102 18-19 0.24780478294520236 1102 20-21 0.11290961069307315 1102 22-23 0.2277000572097876 1102 24-25 0.1906281430742709 1102 26-27 0.06448516091737844 1102 28-29 0.12810643216071327 1102 30-31 0.17065878222882702 1102 32-33 0.13033040339973212 1102 34-35 0.18433528688533585 1102 36-37 0.14715298722817494 1102 38-39 0.11176217738744754 1102 40-41 0.21879176279723822 1102 42-43 0.3019477695730899 1102 44-45 0.3154022266210248 1102 46-47 0.2884407773821991 1102 48-49 0.3238743236830217 1102 50-51 0.22876873435970424 1102 52-53 0.18032912491428021 1102 54-55 0.27662519240258376 1102 56-57 0.33421493255731605 1102 58-59 0.19510211056050508 1102 60-61 0.19328980603257406 1102 62-63 0.17121140462804618 1102 64-65 0.33093023316148873 1102 66-67 0.22126139530631406 1102 68-69 0.4453834791044997 1102 70-71 0.3458367870056307 1102 72-73 0.26443781869389227 1102 74-75 0.3043318240474875 1102 76-77 0.2800180369975962 1102 78-79 0.2063432101015259 1102 80-81 0.35734736369391484 1102 82-83 0.401890353132071 1102 84-85 0.41912575716151323 1102 86-87 0.41334239802408135 1102 88-89 0.20910890945372174 1102 90-91 0.24346109397751903 1102 92-93 0.3872423755625469 1102 94-95 0.4242280710379909 1102 96-97 0.35872808554211844 1102 98-99 0.46669604076179994 1102 100-101 0.7267145332162066 1103 1 0.044726573960907956 1103 2 0.0950139206223426 1103 3 0.07823152724381544 1103 4 0.03150770438199402 1103 5 0.06078563008538396 1103 6 0.02770097335242383 1103 7 0.024361254894330386 1103 8 0.027658105793506138 1103 9 0.024270163412317913 1103 10-11 0.009315535793398055 1103 12-13 0.0606338441145482 1103 14-15 0.09851856983475216 1103 16-17 0.03198924879675502 1103 18-19 0.055230402474570894 1103 20-21 0.12378739765155444 1103 22-23 0.15833325686316613 1103 24-25 0.059423276854992935 1103 26-27 0.004721821471044052 1103 28-29 0.10216445866831236 1103 30-31 0.026641464292204375 1103 32-33 0.10825775942507221 1103 34-35 0.053158298647836943 1103 36-37 0.14187094938129974 1103 38-39 0.040425444955616285 1103 40-41 0.08384217827993723 1103 42-43 0.07535822079333343 1103 44-45 0.10226128494981879 1103 46-47 -0.024106665568702113 1103 48-49 -0.053283196990342674 1103 50-51 0.10532805400259804 1103 52-53 0.014734249088853346 1103 54-55 0.05436076134852641 1103 56-57 0.08757271790357635 1103 58-59 0.07485765760711871 1103 60-61 0.1431578054682987 1103 62-63 0.23743672589659326 1103 64-65 0.037309588676286864 1103 66-67 0.11343440001534333 1103 68-69 0.1527331899124249 1103 70-71 0.13733668960063738 1103 72-73 -0.07454164564580168 1103 74-75 0.05091156515172912 1103 76-77 0.05373615365519413 1103 78-79 0.0835356622219301 1103 80-81 0.04849728005996923 1103 82-83 0.14233022066127532 1103 84-85 0.2593731658527254 1103 86-87 0.2817972860755127 1103 88-89 0.2537150238004706 1103 90-91 0.2588238772077389 1103 92-93 0.1716972098732903 1103 94-95 0.06389575206013731 1103 96-97 0.06303372226850001 1103 98-99 0.14552489682408165 1103 100-101 0.2917861090947014 1104 1 0.16203742195767745 1104 2 0.09841238737821101 1104 3 0.09510268256262577 1104 4 0.1018271863440745 1104 5 0.09263999713830628 1104 6 0.07966801954329128 1104 7 0.06395547535497315 1104 8 0.14089790450262285 1104 9 0.08688723947705057 1104 10-11 0.1287300713180457 1104 12-13 0.17587655868035768 1104 14-15 0.1653906718602869 1104 16-17 0.09202234145619315 1104 18-19 0.1926886421891254 1104 20-21 0.20468517015582677 1104 22-23 0.19495050543889647 1104 24-25 0.17492191780490174 1104 26-27 0.14121946943078711 1104 28-29 0.16556845839902934 1104 30-31 0.2664205029430846 1104 32-33 0.26687100237946737 1104 34-35 0.3705803320897232 1104 36-37 0.37694127917184517 1104 38-39 0.2842894179260824 1104 40-41 0.3015910359204881 1104 42-43 0.4368638631653994 1104 44-45 0.3294408142051708 1104 46-47 0.231262739394559 1104 48-49 0.08857701860633682 1104 50-51 0.28138710325659133 1104 52-53 0.25341279376920056 1104 54-55 0.2767543725508048 1104 56-57 0.5008467032443491 1104 58-59 0.4522445156347672 1104 60-61 0.433595226050123 1104 62-63 0.4953306821208727 1104 64-65 0.5289748878776876 1104 66-67 0.6166491004773391 1104 68-69 0.6760688794936058 1104 70-71 0.568014720955567 1104 72-73 0.514231043758663 1104 74-75 0.47209738895487874 1104 76-77 0.5450837801562507 1104 78-79 0.6833927095067729 1104 80-81 0.6276880351532519 1104 82-83 0.7381829088876124 1104 84-85 0.7857294810177002 1104 86-87 0.7395879460773607 1104 88-89 0.6287133407662928 1104 90-91 0.62998066867139 1104 92-93 0.9906343948303302 1104 94-95 0.8551108396199929 1104 96-97 1.0111374687025716 1104 98-99 1.0767776843494339 1104 100-101 1.029548346856938 1105 1 0.19117819082516974 1105 2 0.19036951615353104 1105 3 0.12403029973266655 1105 4 0.12587093553187856 1105 5 0.0574430259526153 1105 6 0.05517221443209053 1105 7 0.04122510512078037 1105 8 0.11711722729828011 1105 9 0.059734962002664815 1105 10-11 0.07918042361262678 1105 12-13 0.15885908974979657 1105 14-15 0.21444111670550114 1105 16-17 0.16226434390942046 1105 18-19 0.176542599203799 1105 20-21 0.22391438509198025 1105 22-23 0.2401051870108617 1105 24-25 0.2711000840980873 1105 26-27 0.20155051224178777 1105 28-29 0.11681119618598501 1105 30-31 0.28239519256568 1105 32-33 0.3429104932876399 1105 34-35 0.34751498088861865 1105 36-37 0.46808561852836306 1105 38-39 0.3586656916857649 1105 40-41 0.3630912435254885 1105 42-43 0.3208499108416518 1105 44-45 0.40489374175260195 1105 46-47 0.26999870228556944 1105 48-49 0.3501605059265813 1105 50-51 0.40863668566943545 1105 52-53 0.2969700261885464 1105 54-55 0.4374533199459236 1105 56-57 0.49613418862483627 1105 58-59 0.4681787935972679 1105 60-61 0.5215539776309228 1105 62-63 0.47244995673949575 1105 64-65 0.5584272623400537 1105 66-67 0.46842804552698425 1105 68-69 0.48593677298431004 1105 70-71 0.6023719616157095 1105 72-73 0.6029849541810606 1105 74-75 0.5163881722437864 1105 76-77 0.4758286938337726 1105 78-79 0.6253110510122433 1105 80-81 0.6625597320943264 1105 82-83 0.7826248397091895 1105 84-85 0.8088077084830516 1105 86-87 0.8342477036707905 1105 88-89 0.5894153784648282 1105 90-91 0.564341435446984 1105 92-93 0.703302142839064 1105 94-95 0.6741032501517452 1105 96-97 0.9511628012667614 1105 98-99 0.8659478744902565 1105 100-101 0.8087406207814318 1106 1 -0.09887076180191912 1106 2 0.010063249228011273 1106 3 0.004449558039311796 1106 4 -0.005246210716990163 1106 5 0.005705029934361505 1106 6 -0.0061515004648384775 1106 7 0.019830163919579036 1106 8 -0.07266733167981698 1106 9 -0.18422363152513554 1106 10-11 0.006861109351291361 1106 12-13 -0.05882417732411227 1106 14-15 -0.1915190627204879 1106 16-17 -0.1180694366492574 1106 18-19 -0.05087052418093663 1106 20-21 -0.026026491510926064 1106 22-23 -0.009975576322432289 1106 24-25 -0.02101702814517381 1106 26-27 -0.04663327882771995 1106 28-29 -0.046997324384314254 1106 30-31 0.028274910071772297 1106 32-33 -0.01362503794188541 1106 34-35 -0.020331474489829304 1106 36-37 -0.07520333490248987 1106 38-39 -0.0458940747265828 1106 40-41 -0.042695236769802136 1106 42-43 -0.14687412432633096 1106 44-45 -0.0470543935031742 1106 46-47 -0.08957530784192613 1106 48-49 0.0026550109284997347 1106 50-51 -0.06821978899947112 1106 52-53 -0.1286755394793886 1106 54-55 -0.4082819509860869 1106 56-57 -0.2552596551224582 1106 58-59 -0.06411378761432474 1106 60-61 -0.13985347609052212 1106 62-63 -0.034888879835698106 1106 64-65 -0.316576988361696 1106 66-67 -0.2761074841568174 1106 68-69 -0.39930007611732066 1106 70-71 -0.44376341627940263 1106 72-73 -0.2448546073320017 1106 74-75 -0.3242516665696087 1106 76-77 -0.235901658649869 1106 78-79 -0.26062677745107266 1106 80-81 -0.33065112435252075 1106 82-83 -0.40185735526045363 1106 84-85 -0.35617303970228775 1106 86-87 -0.07764317076811622 1106 88-89 -0.04277236498445447 1106 90-91 -0.1888123920394449 1106 92-93 -0.06312952442527475 1106 94-95 -0.16799496579008988 1106 96-97 0.026472484210785296 1106 98-99 0.03989006803784534 1106 100-101 0.03549024826538982 1107 1 0.0919467404035359 1107 2 0.07850961858888184 1107 3 0.06149865608330174 1107 4 0.00882498753080796 1107 5 0.04105014598705026 1107 6 0.039917842156761196 1107 7 -0.018634913803779796 1107 8 0.013377066458474474 1107 9 -0.012969486786296613 1107 10-11 -0.03684183455403911 1107 12-13 0.05063923109617008 1107 14-15 0.17413417093631267 1107 16-17 0.12241401481546887 1107 18-19 0.06068635760654928 1107 20-21 0.11092157377061085 1107 22-23 0.15542479023979183 1107 24-25 0.0652709209315745 1107 26-27 0.0014297212026974648 1107 28-29 0.0758392360147937 1107 30-31 0.04343676402486807 1107 32-33 -0.06117137155913355 1107 34-35 0.0682237227845306 1107 36-37 0.05991733783805131 1107 38-39 0.05502366171366191 1107 40-41 0.054598419878502114 1107 42-43 0.04325835521614607 1107 44-45 0.1208453646570149 1107 46-47 0.06508362578141202 1107 48-49 0.0298006737825105 1107 50-51 -0.0050089092501366395 1107 52-53 0.0033752696953115446 1107 54-55 0.14439283151019566 1107 56-57 0.1759161958798785 1107 58-59 0.045402951046582984 1107 60-61 0.052083580916750805 1107 62-63 0.247967016340489 1107 64-65 0.06237667421853388 1107 66-67 0.031540699763105806 1107 68-69 0.11642996829602481 1107 70-71 0.11031468352894791 1107 72-73 0.11880820105177747 1107 74-75 -0.03782007757559569 1107 76-77 -0.0422850317828356 1107 78-79 0.07779324049678493 1107 80-81 0.1400815057694622 1107 82-83 0.20881715345490548 1107 84-85 0.20995541297924447 1107 86-87 0.14761663591214003 1107 88-89 0.14072786696882034 1107 90-91 0.07478948107830874 1107 92-93 0.15879614405645626 1107 94-95 0.09848369831115278 1107 96-97 0.31668956966085204 1107 98-99 0.4551288278103023 1107 100-101 0.5656174613277791 1108 1 0.07304825228258238 1108 2 0.11358896876260616 1108 3 0.0851262267472137 1108 4 0.08078103974228412 1108 5 0.07904244573350638 1108 6 0.05902761772254195 1108 7 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-0.5531520153271998 2316 58-59 -0.5535628206521963 2316 60-61 -0.5735697189546372 2316 62-63 -0.7012364059675136 2316 64-65 -0.6938578471648 2316 66-67 -0.7681228823477397 2316 68-69 -0.8382871918009513 2316 70-71 -0.767948073983888 2316 72-73 -0.6073653891867075 2316 74-75 -0.477670543043601 2316 76-77 -0.5283025436131545 2316 78-79 -0.7423660750416126 2316 80-81 -0.7532129355906143 2316 82-83 -0.723668231598257 2316 84-85 -0.5411387718469669 2316 86-87 -0.5264508182241663 2316 88-89 -0.6022692283159365 2316 90-91 -0.5186050328561436 2316 92-93 -0.7127681904261145 2316 94-95 -0.5213427671429258 2316 96-97 -0.5131894904575347 2316 98-99 -0.5098323701367313 2316 100-101 -0.2829012905322621 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 8.0 4 19.0 5 32.0 6 78.0 7 178.0 8 465.0 9 898.0 10 1530.0 11 2525.0 12 3967.0 13 5676.0 14 7602.0 15 10331.0 16 13072.0 17 18179.0 18 18042.0 19 13353.0 20 11668.0 21 11323.0 22 11810.0 23 12831.0 24 14768.0 25 16897.0 26 20227.0 27 24324.0 28 30081.0 29 36643.0 30 44886.0 31 55072.0 32 69410.0 33 90287.0 34 126643.0 35 190098.0 36 316371.0 37 518502.0 38 449902.0 39 49263.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.73373245934054 19.060914433398406 14.914470722591647 23.29088238466941 2 15.502596004361196 19.97646410776434 40.32180894045237 24.19913094742209 3 19.32912540217516 23.623227197328482 30.112290400053897 26.935357000442462 4 12.868377176352672 16.88404969214376 37.05185376020668 33.19571937129688 5 13.416041296933454 37.37511760531678 34.715951582340566 14.4928895154092 6 32.40631288499099 36.595315268731845 16.861017939732367 14.137353906544794 7 28.41476453674543 32.79876302192978 20.437084398627743 18.349388042697047 8 26.201341854198528 35.35175879396985 19.449375500691865 18.997523851139757 9 25.90635510266691 16.77364029096583 20.310711305946082 37.00929330042118 10-11 24.920105763991902 26.522451179284896 27.291147295339442 21.266295761383763 12-13 25.85530415340786 23.8113499201399 27.675563629312595 22.657782297139647 14-15 22.31815498366612 25.41456016408075 26.11923620154513 26.148048650708 16-17 21.88528479662261 28.522744471059475 27.175247643052625 22.416723089265293 18-19 21.90424344107126 26.937614447554285 29.491066547654103 21.66707556372035 20-21 22.189909998648137 26.133847253964216 29.921068454828863 21.755174292558788 22-23 22.003949080709905 26.119623098571804 29.802450378180463 22.073977442537824 24-25 21.964724451742782 26.278087122569953 29.810125645954034 21.94706277973323 26-27 21.50114159149008 26.60770910018006 29.700295243103213 22.190854065226645 28-29 21.675137058302894 27.118574634615506 29.418632464354825 21.787655842726778 30-31 21.80676814899814 26.528072565495975 29.385580211263985 22.279579074241898 32-33 21.49938858570417 26.56030128967109 29.453191018206788 22.48711910641795 34-35 22.290880020686874 26.723010777751778 29.19353061739209 21.792578584169263 36-37 22.352970362153357 26.257222151162075 29.237803309865278 22.15200417681929 38-39 21.797581142269735 26.676064635200085 29.196853827824604 22.329500394705573 40-41 22.27919303172593 26.53371066694315 29.16220056845871 22.024895732872203 42-43 21.92961652298759 26.523288027917047 29.026390276011316 22.52070517308405 44-45 22.014636975158655 26.77090284409591 29.17405965309331 22.04040052765212 46-47 22.553347991423628 27.01650207228922 28.369981972870317 22.06016796341683 48-49 22.116695426012317 27.050724931234743 28.42643972349152 22.406139919261417 50-51 22.239114196981564 27.30780662613503 28.39648934189726 22.05658983498614 52-53 22.6697533463179 27.02097937747813 28.2725912587672 22.036676017436765 54-55 22.08289883279995 27.24194309700601 28.42270730511655 22.25245076507749 56-57 22.218312792135702 27.36615980121686 28.183718735875413 22.231808670772025 58-59 22.239387300765102 27.016086268023145 28.176276657774103 22.568249773437653 60-61 21.8787082373108 27.25384587023838 28.54180331338614 22.325642579064684 62-63 21.95058004967758 27.269936234818275 28.657531041658796 22.121952673845353 64-65 21.651053475086336 27.396406045243282 28.412033498910088 22.540506980760295 66-67 21.626496893216878 27.357465997441015 28.340389273029594 22.675647836312514 68-69 22.22930271163273 26.95228248300322 28.47311091847122 22.34530388689282 70-71 21.998441487741964 27.14307952736659 28.05467719815546 22.803801786735985 72-73 21.980712500460864 27.215452030003185 28.144323515100588 22.659511954435366 74-75 22.01664840664425 27.616140797745253 27.84384107726698 22.523369718343517 76-77 21.988541475588846 27.163266448699503 28.150422832932858 22.697769242778794 78-79 22.09125125684637 27.36499911013684 28.0725199786797 22.471229654337094 80-81 22.336794316892334 27.41292882414711 27.71056643090224 22.539710428058317 82-83 22.25743747793831 27.497872632898712 27.85452382139083 22.390166067772146 84-85 22.19715663367932 27.57135840772214 27.819571460369435 22.411913498229097 86-87 22.33556315646094 27.592439645592172 27.542324953043078 22.52967224490381 88-89 22.172896621977763 27.73113011307412 27.37384192563745 22.722131339310668 90-91 22.099699494119605 27.636504409270618 27.53657095571066 22.727225140899115 92-93 22.31633204147275 27.277299705661527 27.707917370385132 22.698450882480593 94-95 21.973657319386227 27.134385723590746 27.950169483656335 22.94178747336669 96-97 22.219319229492122 26.874966004260877 27.91897373036867 22.986741035878335 98-99 22.002082871522422 27.17382646166283 27.50173307109301 23.322357595721737 100-101 22.041455333648617 26.838204670347803 27.94602740960606 23.17431258639752 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 534.0 1 374.5 2 333.0 3 467.0 4 396.0 5 369.0 6 719.0 7 1635.5 8 2652.0 9 2982.0 10 2477.5 11 1963.0 12 1924.5 13 2032.0 14 2250.0 15 2404.0 16 2518.5 17 2729.0 18 2986.5 19 3380.5 20 3911.5 21 4699.5 22 5729.0 23 7165.0 24 9127.0 25 11556.0 26 14738.5 27 18385.5 28 21350.5 29 24402.5 30 27917.0 31 32380.0 32 39547.0 33 43090.5 34 43095.5 35 45548.0 36 49713.0 37 54036.5 38 57644.0 39 60569.5 40 63990.5 41 68415.0 42 70413.0 43 71758.5 44 74324.5 45 78393.5 46 80057.5 47 81748.0 48 84082.5 49 81241.5 50 78185.5 51 75780.5 52 74110.0 53 74308.5 54 74753.5 55 70660.5 56 63935.5 57 58553.0 58 54404.0 59 47716.5 60 37095.0 61 30167.5 62 26007.0 63 18944.0 64 12602.5 65 9437.0 66 6953.0 67 5186.5 68 3984.0 69 2948.0 70 2144.5 71 1494.5 72 1031.5 73 814.0 74 608.5 75 445.0 76 320.0 77 191.0 78 121.5 79 77.0 80 40.0 81 18.0 82 11.0 83 7.5 84 8.0 85 5.0 86 1.0 87 1.5 88 2.0 89 1.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.014656569716340755 3 0.007419319452681811 4 0.0 5 0.0 6 0.0 7 0.0 8 3.1862108079001644E-4 9 3.64138378045733E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.0482783765072486E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.005439317022058137 26-27 0.02148416430469825 28-29 2.2758648627858314E-5 30-31 0.0 32-33 5.234489184407412E-4 34-35 0.018434505388565236 36-37 0.003641383780457331 38-39 0.017888297821496636 40-41 0.014815880256735765 42-43 0.007874492425238976 44-45 0.002958624321621581 46-47 2.2758648627858314E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.958624321621581E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.1379324313929159E-4 84-85 0.002913107024365864 86-87 2.958624321621581E-4 88-89 4.551729725571663E-5 90-91 2.2758648627858318E-4 92-93 2.958624321621581E-4 94-95 0.0 96-97 2.2758648627858314E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2196967.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.151658695712605 #Duplication Level Percentage of deduplicated Percentage of total 1 76.60650759636476 43.78188976017954 2 13.740534977523977 15.70588730663903 3 4.376896786694025 7.504407338984944 4 1.8475654041954708 4.223657095943434 5 0.9672296508298756 2.763938944230116 6 0.5822616495292299 1.9966331439298304 7 0.3774976693531999 1.5102232570110734 8 0.2515348662013055 1.1500507858567 9 0.18191301395067241 0.935696743705551 >10 0.9693894494758843 10.053844963665325 >50 0.06146484164924721 2.374819667355472 >100 0.03131235145445574 3.42225011377032 >500 0.00302548953460405 1.1881312054532736 >1k 0.00270701695201391 2.6660260935047564 >5k 1.592362912949359E-4 0.722543579770644 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8597 0.3913122045073959 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7282 0.3314569586161285 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4760 0.21666233493721115 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3082 0.14028431014211865 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2868 0.1305436085293953 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2752 0.12526360204773218 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2674 0.12171325286178628 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2525 0.11493117557068448 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2240 0.10195874585280525 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 9.103459451143326E-5 2 0.0 0.0 0.0 0.0 9.103459451143326E-5 3 0.0 0.0 0.0 0.0 9.103459451143326E-5 4 0.0 0.0 0.0 0.0 9.103459451143326E-5 5 0.0 0.0 0.0 0.0 9.103459451143326E-5 6 0.0 0.0 0.0 0.0 1.365518917671499E-4 7 0.0 0.0 0.0 0.0 1.365518917671499E-4 8 0.0 0.0 0.0 0.0 1.365518917671499E-4 9 0.0 0.0 0.0 0.0 1.365518917671499E-4 10-11 0.0 0.0 0.0 0.0 1.365518917671499E-4 12-13 4.551729725571663E-5 0.0 0.0 0.0 1.365518917671499E-4 14-15 4.551729725571663E-5 0.0 0.0 0.0 1.365518917671499E-4 16-17 4.551729725571663E-5 0.0 0.0 0.0 1.365518917671499E-4 18-19 4.551729725571663E-5 0.0 0.0 9.103459451143326E-5 1.820691890228665E-4 20-21 4.551729725571663E-5 0.0 0.0 9.103459451143326E-5 2.503451349064415E-4 22-23 4.551729725571663E-5 0.0 0.0 1.3655189176714987E-4 2.731037835342998E-4 24-25 4.551729725571663E-5 0.0 0.0 3.1862108079001644E-4 3.1862108079001644E-4 26-27 4.551729725571663E-5 0.0 0.0 0.0011151737827650575 3.64138378045733E-4 28-29 1.365518917671499E-4 0.0 0.0 0.0032089694565280223 3.64138378045733E-4 30-31 1.365518917671499E-4 0.0 0.0 0.010742082152349125 3.64138378045733E-4 32-33 1.365518917671499E-4 0.0 0.0 0.028562104027962186 3.64138378045733E-4 34-35 1.365518917671499E-4 0.0 0.0 0.05448420481509281 4.0965567530144967E-4 36-37 1.365518917671499E-4 0.0 0.0 0.09808977558606934 4.0965567530144967E-4 38-39 1.365518917671499E-4 0.0 0.0 0.1630884760672327 4.0965567530144967E-4 40-41 1.365518917671499E-4 0.0 0.0 0.2278368314134896 4.0965567530144967E-4 42-43 1.365518917671499E-4 0.0 0.0 0.2953162245950895 4.0965567530144967E-4 44-45 1.820691890228665E-4 0.0 0.0 0.37991012154483883 4.551729725571663E-4 46-47 2.0482783765072483E-4 0.0 0.0 0.4746771344312409 5.006902698128829E-4 48-49 2.2758648627858315E-4 0.0 0.0 0.5738820838000753 5.006902698128829E-4 50-51 2.2758648627858315E-4 0.0 0.0 0.6823042858631923 5.462075670685996E-4 52-53 2.2758648627858315E-4 0.0 0.0 0.7826699263120476 5.462075670685996E-4 54-55 2.2758648627858315E-4 0.0 0.0 0.8878831589186365 5.917248643243162E-4 56-57 2.2758648627858315E-4 0.0 0.0 1.015172280694248 6.372421615800329E-4 58-59 2.2758648627858315E-4 0.0 0.0 1.1484014097617306 6.827594588357495E-4 60-61 2.2758648627858315E-4 0.0 0.0 1.2884581334175707 6.827594588357495E-4 62-63 2.2758648627858315E-4 0.0 0.0 1.4303583076122672 7.737940533471827E-4 64-65 2.2758648627858315E-4 0.0 0.0 1.573055034508939 8.193113506028993E-4 66-67 2.2758648627858315E-4 0.0 0.0 1.7371448911157974 8.193113506028993E-4 68-69 2.2758648627858315E-4 0.0 0.0 1.9187361485174788 8.193113506028993E-4 70-71 2.503451349064415E-4 0.0 0.0 2.105721205643963 8.193113506028993E-4 72-73 2.731037835342998E-4 0.0 0.0 2.294617989255187 8.420699992307577E-4 74-75 2.731037835342998E-4 0.0 0.0 2.483992704487596 9.103459451143326E-4 76-77 2.731037835342998E-4 0.0 0.0 2.686362608086512 9.558632423700492E-4 78-79 2.731037835342998E-4 0.0 0.0 2.9139035770678396 9.786218909979074E-4 80-81 2.731037835342998E-4 0.0 0.0 3.154257665226651 0.0010241391882536241 82-83 2.731037835342998E-4 0.0 0.0 3.3974793431125727 0.001228967025904349 84-85 2.731037835342998E-4 0.0 0.0 3.6494175834229643 0.001228967025904349 86-87 2.731037835342998E-4 0.0 0.0 3.9047241037302793 0.0012517256745322074 88-89 2.731037835342998E-4 0.0 0.0 4.1797396137493195 0.001297242971787924 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5540 0.0 42.19283 1 GTATCAA 8525 0.0 33.71664 1 ATCAACG 10435 0.0 27.402393 3 TATCAAC 10565 0.0 27.116343 2 TCAACGC 10545 0.0 27.114697 4 CAACGCA 10760 0.0 26.572905 5 AACGCAG 10975 0.0 26.052341 6 ACGCAGA 12240 0.0 23.24343 7 CGCAGAG 12345 0.0 23.00726 8 GTACATG 6085 0.0 22.564232 1 TACATGG 5940 0.0 22.475182 2 GAGTACT 8145 0.0 22.392616 12-13 ACATGGG 5980 0.0 21.446037 3 TAAGGTG 1535 0.0 21.349884 5 GTACTTT 8595 0.0 20.971554 14-15 TACACCG 275 1.2623786E-9 20.72542 5 GCAGAGT 13645 0.0 20.710865 9 GTAAGGT 1575 0.0 20.506105 4 AGAGTAC 11010 0.0 20.404858 10-11 GGACCGA 485 0.0 19.585878 6 >>END_MODULE