FastQCFastQC Report
Thu 26 May 2016
SRR937992_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937992_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2145798
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83710.39011127794880973No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69870.32561312854238844No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43090.20081107354932756No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA30470.14199845465416597No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA27550.12839046359442968No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA26090.12158646806456153No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC23220.10821149055036867No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA23160.10793187429571655No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52900.041.6658331
GTATCAA86950.037.996591
TCAACGC105400.030.8605234
ATCAACG105250.030.8593223
TATCAAC106950.030.546662
CAACGCA107100.030.4150755
AACGCAG111250.029.3170726
ACGCAGA124650.026.0879557
CGCAGAG125750.025.7817278
TACATGG65600.023.1971132
GCAGAGT137550.023.119639
GTACATG67400.023.0963081
GAGTACT82450.022.97540512-13
GTAAGGT13700.022.9092644
ACATGGG66400.021.98663
TAAGGTG15450.021.8533335
GTACTTT87550.021.52818714-15
AGAGTAC113200.021.14333310-11
AGGTAAG14650.020.4498772
AGTACTT86150.020.30570212-13
Produced by FastQC (version 0.11.5)