FastQCFastQC Report
Thu 26 May 2016
SRR937992_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937992_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2145798
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81790.38116355779994204No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68660.31997420074023747No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44510.207428658242761No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA29770.13873626501655795No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA28460.13263131012332008No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA27140.12647975252097354No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC24940.11622715651706265No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA24740.11529510233488893No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG22010.10257256274821767No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52150.041.45231
GTATCAA82550.035.2229651
ATCAACG99550.028.9128763
TCAACGC100700.028.5800274
TATCAAC102250.028.3404982
CAACGCA101500.028.2611835
AACGCAG105500.027.1446536
GTACATG63450.024.9347021
TAAGGTG16600.024.6042335
TACATGG61500.024.4864062
ACGCAGA117300.024.1300167
CGCAGAG117900.023.9266538
ACATGGG62050.023.4228423
GTAAGGT17150.022.9844154
GAGTACT80350.022.66672912-13
GGTAAGG16350.022.0777993
GCAGAGT129050.021.4913739
GTACTTT83600.021.3305914-15
GTATAGG5800.021.2979051
AGGTAAG16150.021.1787782
Produced by FastQC (version 0.11.5)