##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937992_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2145798 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.491874817666904 34.0 31.0 34.0 31.0 34.0 2 32.709274125523464 34.0 31.0 34.0 31.0 34.0 3 32.76512980252568 34.0 31.0 34.0 31.0 34.0 4 36.226954727332206 37.0 35.0 37.0 35.0 37.0 5 36.201529221296695 37.0 35.0 37.0 35.0 37.0 6 36.23105157148996 37.0 37.0 37.0 35.0 37.0 7 36.18959939379196 37.0 37.0 37.0 35.0 37.0 8 36.173454351248346 37.0 37.0 37.0 35.0 37.0 9 37.866457606913606 39.0 38.0 39.0 35.0 39.0 10-11 37.836842983356306 39.0 38.0 39.0 35.0 39.0 12-13 37.7594139802535 39.0 38.0 39.0 35.0 39.0 14-15 39.027734670271855 40.0 38.0 41.0 36.0 41.0 16-17 38.90919765047782 40.0 38.0 41.0 35.0 41.0 18-19 39.1657131752383 40.0 38.5 41.0 36.0 41.0 20-21 39.1415913799901 40.0 39.0 41.0 36.0 41.0 22-23 39.04865835460747 40.0 39.0 41.0 35.5 41.0 24-25 38.943274483432276 40.0 38.0 41.0 35.0 41.0 26-27 38.76134985678988 40.0 38.0 41.0 35.0 41.0 28-29 38.64630361292163 40.0 38.0 41.0 34.5 41.0 30-31 38.40258309496048 40.0 38.0 41.0 34.0 41.0 32-33 38.185751175087304 40.0 38.0 41.0 34.0 41.0 34-35 38.0226528778571 40.0 38.0 41.0 33.0 41.0 36-37 38.00964606174486 40.0 38.0 41.0 33.0 41.0 38-39 37.84849995199921 40.0 37.0 41.0 33.0 41.0 40-41 37.674034787990294 40.0 37.0 41.0 33.0 41.0 42-43 37.433488846573624 40.0 37.0 41.0 32.5 41.0 44-45 37.16414895530707 40.0 36.5 41.0 31.5 41.0 46-47 36.778026869257964 39.5 36.0 41.0 31.0 41.0 48-49 36.37546358044886 39.0 35.5 41.0 30.0 41.0 50-51 35.97894629410597 39.0 35.0 40.5 29.0 41.0 52-53 35.45550559745139 38.5 35.0 40.0 27.5 41.0 54-55 35.1310456529459 38.0 34.0 40.0 27.0 41.0 56-57 34.76758273611962 38.0 34.0 40.0 26.0 41.0 58-59 34.31026662341935 37.5 33.5 40.0 25.5 41.0 60-61 33.80670804055181 37.0 33.0 40.0 23.5 41.0 62-63 33.3269459660229 36.5 32.5 39.0 22.5 40.5 64-65 32.823518336767954 36.0 32.0 39.0 21.5 40.0 66-67 32.246452135755554 35.0 31.0 38.5 18.5 40.0 68-69 31.639630104977265 35.0 31.0 38.0 16.0 40.0 70-71 30.99182355468688 35.0 30.0 37.5 10.5 39.5 72-73 30.378185644687896 34.0 29.0 37.0 7.5 39.0 74-75 29.753902743874306 34.0 29.0 36.0 6.5 39.0 76-77 28.40375981336547 32.5 27.0 35.0 2.0 37.0 78-79 29.070937012710424 34.0 29.0 35.0 2.0 37.0 80-81 28.71801003635943 34.0 28.0 35.0 2.0 37.0 82-83 28.1139403615811 33.0 26.5 35.0 2.0 36.5 84-85 27.470996337959118 33.0 26.0 35.0 2.0 36.0 86-87 26.781757882149204 32.5 25.0 35.0 2.0 35.5 88-89 25.980784770980307 32.0 23.5 34.0 2.0 35.0 90-91 25.379169660890724 31.5 20.5 34.0 2.0 35.0 92-93 24.51210202451489 31.0 17.5 34.0 2.0 35.0 94-95 23.625045321134607 31.0 4.5 34.0 2.0 35.0 96-97 22.503812101605092 30.0 2.0 34.0 2.0 35.0 98-99 20.975688298712182 29.5 2.0 34.0 2.0 35.0 100-101 16.65640241998548 14.5 2.0 30.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.15199692643314933 1101 2 0.12017138210389078 1101 3 0.11185002612204187 1101 4 0.10911749071851062 1101 5 0.0820065939333503 1101 6 0.03738727799863284 1101 7 0.11454291867310928 1101 8 0.10079899700802031 1101 9 0.05409678713888866 1101 10-11 0.10311950117682755 1101 12-13 0.23642301213132555 1101 14-15 0.09734680016051556 1101 16-17 0.2525357407437454 1101 18-19 0.24003496282961834 1101 20-21 0.13581746501505165 1101 22-23 0.1883943661143519 1101 24-25 0.22736929252818072 1101 26-27 0.13344584898327128 1101 28-29 0.2944155298723601 1101 30-31 0.24384991496716424 1101 32-33 0.22506345726058186 1101 34-35 0.2680217721298348 1101 36-37 0.42869585208732985 1101 38-39 0.4401593544643845 1101 40-41 0.3889925477970877 1101 42-43 0.49849911274104386 1101 44-45 0.4563694333853121 1101 46-47 0.5195244082664701 1101 48-49 0.5994518412868857 1101 50-51 0.5088112516355139 1101 52-53 0.2778139654932872 1101 54-55 0.09033395865122174 1101 56-57 0.24545641134499618 1101 58-59 0.45274373060483697 1101 60-61 0.303716173018465 1101 62-63 0.3653228838385374 1101 64-65 0.5109062046020583 1101 66-67 0.3383711752011749 1101 68-69 0.26557741997134343 1101 70-71 0.20801967625411066 1101 72-73 0.234170374003714 1101 74-75 0.013871782391952081 1101 76-77 0.02759926778118782 1101 78-79 0.13477742699118878 1101 80-81 -0.0578543987486313 1101 82-83 -0.10842227522120496 1101 84-85 -0.10200342254650963 1101 86-87 -0.10298791086314907 1101 88-89 -0.15282534878342702 1101 90-91 -0.7344300767594021 1101 92-93 -0.8005830508430236 1101 94-95 -0.8377620177469076 1101 96-97 -0.76334801349876 1101 98-99 -0.7511459522606039 1101 100-101 -0.8853556721781288 1102 1 0.20148200621320456 1102 2 0.2556597329124628 1102 3 0.22112346379037717 1102 4 0.09195716004501975 1102 5 0.1369397112502071 1102 6 0.08952391218416267 1102 7 0.17627588659794924 1102 8 0.11560147839301749 1102 9 0.22711244354545812 1102 10-11 0.2563408435092498 1102 12-13 0.21476899316797926 1102 14-15 0.2955027167923774 1102 16-17 0.22558156284616615 1102 18-19 0.34461373582454513 1102 20-21 0.2812211210153066 1102 22-23 0.3099245415576348 1102 24-25 0.33095741996696404 1102 26-27 0.29684311170288424 1102 28-29 0.27858341325629254 1102 30-31 0.23603120259091526 1102 32-33 0.23701289483452115 1102 34-35 0.20636639878829754 1102 36-37 0.20875958481516932 1102 38-39 0.1978594304942547 1102 40-41 0.3350225011018182 1102 42-43 0.2439584829346657 1102 44-45 0.10162187613975249 1102 46-47 0.3204355610577778 1102 48-49 0.11158096482976987 1102 50-51 0.19090034406875134 1102 52-53 0.25676621032382485 1102 54-55 0.3924073628705216 1102 56-57 0.31016093117175814 1102 58-59 0.28825488524302045 1102 60-61 0.20410614672095306 1102 62-63 0.31750127801800687 1102 64-65 0.30795904689806264 1102 66-67 0.29652422403984957 1102 68-69 0.4736394073981991 1102 70-71 0.3720228180010743 1102 72-73 0.318522996623674 1102 74-75 0.36043073837158346 1102 76-77 0.18739319195858428 1102 78-79 0.10986135739667091 1102 80-81 0.10312281076033614 1102 82-83 0.23214958531477947 1102 84-85 -0.012203531922807542 1102 86-87 -0.23327332041377247 1102 88-89 -0.2561865922257205 1102 90-91 -0.4267100954744478 1102 92-93 -0.551575876742838 1102 94-95 -0.7502183980480943 1102 96-97 -0.8325646904087698 1102 98-99 -0.9064847930565278 1102 100-101 -1.4112577701304545 1103 1 0.10111159753416388 1103 2 0.15328261862635628 1103 3 0.10802050071610125 1103 4 0.10034160834491246 1103 5 0.04119599580525346 1103 6 0.07254570931027615 1103 7 0.02581638858172397 1103 8 0.07542199216429424 1103 9 0.15657671706240706 1103 10-11 0.09245037167728754 1103 12-13 0.11436662747040316 1103 14-15 0.19563020334116032 1103 16-17 0.1816574946738001 1103 18-19 0.21553811820027846 1103 20-21 0.17840124345703146 1103 22-23 0.20837318687278383 1103 24-25 0.25357996415546324 1103 26-27 0.2242460355505358 1103 28-29 0.24220266514004862 1103 30-31 0.13861401273988605 1103 32-33 0.25758857978808436 1103 34-35 0.2010666565911805 1103 36-37 0.17177945964122898 1103 38-39 0.13844011147636337 1103 40-41 0.2563177312606655 1103 42-43 0.2623259623016523 1103 44-45 0.20152781028975397 1103 46-47 0.24430345318258873 1103 48-49 0.26811912687674067 1103 50-51 0.11888605659805052 1103 52-53 0.1468257723516686 1103 54-55 0.09022106369818772 1103 56-57 0.05789832546965812 1103 58-59 0.14087667807567072 1103 60-61 -0.026392997514001593 1103 62-63 0.062299846343570664 1103 64-65 0.09728667122794121 1103 66-67 -0.036056127126322224 1103 68-69 0.14698257015748695 1103 70-71 -0.1257831253213233 1103 72-73 -0.35760506173618367 1103 74-75 -0.29072498354478427 1103 76-77 -0.05466170775980217 1103 78-79 -0.04778261507052761 1103 80-81 -0.17915353402027634 1103 82-83 -0.07618563453975113 1103 84-85 -0.26844124920613766 1103 86-87 -0.4888277429724006 1103 88-89 -0.6076462229489543 1103 90-91 -0.6508456459255534 1103 92-93 -0.8163866131301205 1103 94-95 -0.9313746859208187 1103 96-97 -1.128435453298426 1103 98-99 -1.3766205933090596 1103 100-101 -1.5888048664815972 1104 1 0.07316956844346123 1104 2 0.04277296421797416 1104 3 0.07032108167730655 1104 4 0.0049303217467553395 1104 5 -0.09389948670647641 1104 6 -0.10115288950530044 1104 7 -0.09651709204096193 1104 8 -0.019239842685941255 1104 9 0.12604109496755456 1104 10-11 0.05752328897719394 1104 12-13 0.07561108030257202 1104 14-15 0.12710481770468363 1104 16-17 0.08342601026031105 1104 18-19 0.08322971608402696 1104 20-21 0.06143255801899272 1104 22-23 0.04212668801919506 1104 24-25 0.022404041699225274 1104 26-27 0.032089011799442346 1104 28-29 -0.0611857913069187 1104 30-31 -2.0332336745099155E-4 1104 32-33 0.048734251976433995 1104 34-35 -0.046378084952308996 1104 36-37 -0.15932850405481958 1104 38-39 0.010456481462362888 1104 40-41 0.01994282755069321 1104 42-43 -0.03112772185252055 1104 44-45 -0.16968557568062437 1104 46-47 -0.13849082231261178 1104 48-49 -0.08459092149283975 1104 50-51 0.04831306172172134 1104 52-53 -0.10144667785652217 1104 54-55 -0.07008108512332711 1104 56-57 -0.07666070020678006 1104 58-59 -0.17667242417005724 1104 60-61 -0.2721054258011719 1104 62-63 -0.20156682564607564 1104 64-65 -0.26084714855341673 1104 66-67 -0.7311544767900173 1104 68-69 -0.6027831127554641 1104 70-71 -0.8030105440044473 1104 72-73 -0.8675400608606161 1104 74-75 -0.7670972923007575 1104 76-77 -0.7199815993524261 1104 78-79 -0.8646745860469665 1104 80-81 -0.8327042658245247 1104 82-83 -0.847983928584334 1104 84-85 -0.7976011373872112 1104 86-87 -0.9143087848373561 1104 88-89 -1.1506918369776749 1104 90-91 -1.1997875216780685 1104 92-93 -1.2494683296831148 1104 94-95 -1.1954604772774466 1104 96-97 -1.4612516860966487 1104 98-99 -1.7012032911357444 1104 100-101 -2.138271630655625 1105 1 0.15737241639968147 1105 2 0.18696522114311165 1105 3 0.19759062735090538 1105 4 0.10175414842086639 1105 5 0.11612472323712097 1105 6 0.14480633065575432 1105 7 0.18078965768781785 1105 8 0.15054937220145348 1105 9 0.13538317959181256 1105 10-11 0.165797466479205 1105 12-13 0.1129660926260101 1105 14-15 0.16027366381970864 1105 16-17 0.17987528407123676 1105 18-19 0.2853489927655417 1105 20-21 0.30166665738155984 1105 22-23 0.3142763177918724 1105 24-25 0.2739462732172413 1105 26-27 0.2553787721861056 1105 28-29 0.3426819230445872 1105 30-31 0.317978514674536 1105 32-33 0.33001591529262697 1105 34-35 0.26772321319177195 1105 36-37 0.3434294677277734 1105 38-39 0.2983494561220894 1105 40-41 0.2457029546780234 1105 42-43 0.13012990192031992 1105 44-45 0.23197851386427715 1105 46-47 0.021739484552036004 1105 48-49 0.21322328018852943 1105 50-51 0.35629098309205176 1105 52-53 0.1932181776156625 1105 54-55 0.07389904533335567 1105 56-57 -0.033517322375679726 1105 58-59 0.0195230406490694 1105 60-61 -0.04845487564866602 1105 62-63 0.055127173512410366 1105 64-65 -0.01761302166464418 1105 66-67 0.033165698294062906 1105 68-69 0.046839232222904315 1105 70-71 -0.1449672588448685 1105 72-73 -0.23704863963949308 1105 74-75 -0.15549080677975624 1105 76-77 -0.17870519659788542 1105 78-79 -0.4056496206622491 1105 80-81 -0.6062813254065027 1105 82-83 -0.604586501453376 1105 84-85 -0.7023547145909781 1105 86-87 -0.766096720412083 1105 88-89 -0.864197455161392 1105 90-91 -0.9658723224561427 1105 92-93 -0.9565809891548156 1105 94-95 -1.169969036394754 1105 96-97 -1.0678871811683663 1105 98-99 -1.2374097402173057 1105 100-101 -1.8264061804428664 1106 1 0.2128476160348569 1106 2 0.19610200169834968 1106 3 0.2091469870077418 1106 4 0.1596599684291391 1106 5 0.1853519549156033 1106 6 0.12956321341209076 1106 7 0.15660978664473646 1106 8 0.15457943629988335 1106 9 0.13365560576229285 1106 10-11 0.20733280406864907 1106 12-13 0.21952001164829227 1106 14-15 0.2137595087812585 1106 16-17 0.305099351269952 1106 18-19 0.30235717280708485 1106 20-21 0.3803174859006049 1106 22-23 0.3383550199751397 1106 24-25 0.32860493230873544 1106 26-27 0.22537886719238998 1106 28-29 0.2570902431305342 1106 30-31 0.1984381345620747 1106 32-33 0.2887069237689843 1106 34-35 0.24112594562401313 1106 36-37 0.20073438579255054 1106 38-39 0.13426884354208113 1106 40-41 0.03250909742522623 1106 42-43 0.13470137348301847 1106 44-45 0.04139409936365013 1106 46-47 0.13144966658151702 1106 48-49 -0.009796281606512025 1106 50-51 0.045939457252522686 1106 52-53 -0.04764574795692056 1106 54-55 -0.19742312638717152 1106 56-57 -0.10442706408952773 1106 58-59 0.029139859740915597 1106 60-61 -0.18114502496003837 1106 62-63 -0.13616657848882596 1106 64-65 -0.17917237273574216 1106 66-67 -0.25417607450790314 1106 68-69 -0.48081491612402516 1106 70-71 -0.547145573325448 1106 72-73 -0.6624640803184612 1106 74-75 -0.661065750180402 1106 76-77 -0.6283408217996111 1106 78-79 -0.45820799881758134 1106 80-81 -0.6108082467225486 1106 82-83 -0.8264210523650668 1106 84-85 -0.7690701853895128 1106 86-87 -1.1104289147826236 1106 88-89 -1.1649206070900568 1106 90-91 -1.2321622421090623 1106 92-93 -1.1341857470698002 1106 94-95 -1.3258652422800594 1106 96-97 -1.6959403816092973 1106 98-99 -1.5300938193970879 1106 100-101 -2.04015056062825 1107 1 -0.05752510115453191 1107 2 0.01298961016138378 1107 3 0.020799574462124326 1107 4 0.014140711417553575 1107 5 -0.04759893942797788 1107 6 -0.017177375697436048 1107 7 -0.015336712270013209 1107 8 -0.01721837256010872 1107 9 -0.04108654895217967 1107 10-11 0.010149054972629301 1107 12-13 -0.1149117272453708 1107 14-15 -0.07694898872576772 1107 16-17 -0.03557819467020806 1107 18-19 -0.004670352761834806 1107 20-21 -0.06579485827234066 1107 22-23 -0.03172533627774499 1107 24-25 0.013134710025354934 1107 26-27 -0.040727317300621735 1107 28-29 -0.06557907560745235 1107 30-31 -0.07766775066569664 1107 32-33 -0.09054449838894385 1107 34-35 -0.1702927200912825 1107 36-37 -0.21491652671902273 1107 38-39 -0.1560133777223882 1107 40-41 -0.3442200135661295 1107 42-43 -0.22276200717271166 1107 44-45 -0.2851664923049526 1107 46-47 -0.14794189182928363 1107 48-49 -0.08638544485853572 1107 50-51 -0.3221338189030831 1107 52-53 -0.29924581553903096 1107 54-55 -0.21881882408403186 1107 56-57 -0.23009054338496782 1107 58-59 -0.3354628821413925 1107 60-61 -0.42370044852993516 1107 62-63 -0.45903772453568337 1107 64-65 -0.4103639644663062 1107 66-67 -0.5791539771863725 1107 68-69 -0.41988828253048993 1107 70-71 -0.6724517016414282 1107 72-73 -0.8127442467853463 1107 74-75 -0.6868501609118134 1107 76-77 -0.8619744886212537 1107 78-79 -0.7668566230104155 1107 80-81 -0.7214104367314711 1107 82-83 -0.5749865232353635 1107 84-85 -0.6623810651892725 1107 86-87 -0.9128377407980572 1107 88-89 -1.0583255080179477 1107 90-91 -1.2485193263418637 1107 92-93 -1.3992039727944992 1107 94-95 -1.6318015100937053 1107 96-97 -1.6571062388852233 1107 98-99 -1.6804876830928457 1107 100-101 -2.0250031442181005 1108 1 -0.03886138979403597 1108 2 -0.05517923454154783 1108 3 -0.07550833042168392 1108 4 0.010224844566629088 1108 5 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1.2103466250512547 2316 76-77 1.0245658466925782 2316 78-79 1.0338509748983782 2316 80-81 1.0710156186879551 2316 82-83 1.303914673940561 2316 84-85 1.2784595486359365 2316 86-87 1.5877825148093407 2316 88-89 1.6773806093743922 2316 90-91 1.831720943959958 2316 92-93 1.8555733932950105 2316 94-95 2.34487695168691 2316 96-97 2.6673843899266956 2316 98-99 3.003298238039836 2316 100-101 3.2463078054492414 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 7.0 4 14.0 5 42.0 6 89.0 7 182.0 8 523.0 9 1006.0 10 1717.0 11 2872.0 12 4491.0 13 6716.0 14 9245.0 15 12473.0 16 16110.0 17 21926.0 18 22742.0 19 18836.0 20 18374.0 21 19124.0 22 20283.0 23 22471.0 24 24908.0 25 28773.0 26 32778.0 27 38559.0 28 46416.0 29 55152.0 30 66816.0 31 80676.0 32 99607.0 33 127955.0 34 171339.0 35 237962.0 36 331134.0 37 392828.0 38 202524.0 39 9124.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.6908169961447 19.010671061364196 14.953095055219572 23.345416887271533 2 15.48468436556173 19.91475260076811 40.30915582236474 24.29140721130542 3 19.253004276643264 23.692212184145472 30.130573711507413 26.924209827703848 4 12.807222677634464 16.85052220690028 37.08617357559918 33.256081539866074 5 13.396235032263295 37.37593101256325 34.703618540932865 14.52421541424059 6 32.33272204888256 36.565870754116844 16.94811383405841 14.153293362942183 7 28.443078053013377 32.785285474215186 20.44036763945162 18.331268833319818 8 26.202326593649545 35.31096589706953 19.438782215287738 19.04792529399319 9 25.90686541790047 16.800742660772354 20.30545279658197 36.9869391247452 10-11 24.88095337958186 26.49569064748872 27.307393333389257 21.31596263954016 12-13 25.852019588178493 23.819126249426784 27.66457388068883 22.664280281705896 14-15 22.363335225403322 25.389598648148613 26.100289962056074 26.146776164391987 16-17 21.887212415847852 28.533166739796012 27.12330446141812 22.45631638293802 18-19 21.8859603746485 26.96043616407509 29.445292613750222 21.70831084752619 20-21 22.208171505425952 26.097447196800445 29.90288461448841 21.791496683285192 22-23 21.98571347349564 26.098938483491924 29.766967813372926 22.148380229639507 24-25 21.94725132146662 26.29618375539795 29.762918961543015 21.993645961592414 26-27 21.56679589679768 26.622176076927467 29.585682839660247 22.2253451866146 28-29 21.67753589922723 27.094449770443486 29.380693763547704 21.84732056678158 30-31 21.819078735043874 26.512483254568544 29.349740147254938 22.31869786313265 32-33 21.484967364122813 26.602690467602265 29.39426264727621 22.518079520998715 34-35 22.278981712833176 26.73591060415184 29.172847912684684 21.8122597703303 36-37 22.38429215649403 26.232214230572872 29.18430884039218 22.199184772540914 38-39 21.855345508928036 26.68323592717759 29.184400443576468 22.277018120317905 40-41 22.376371574660567 26.4793339592204 29.09845930650501 22.04583515961402 42-43 21.9748995041092 26.511551172803532 28.975229694074457 22.538319629012815 44-45 22.0563165777953 26.70929416468838 29.131283559775895 22.10310569774042 46-47 22.58345034502332 26.988270558678195 28.3322700143583 22.09600908194018 48-49 22.2103177552846 27.05103699739 28.327235724523387 22.41140952280201 50-51 22.25196380676747 27.329013074956716 28.32740288157039 22.09162023670542 52-53 22.671386998208437 27.00639724237187 28.198388152191544 22.123827607228147 54-55 22.11847922769662 27.273926325255456 28.302247331615582 22.30534711543234 56-57 22.275177529755315 27.36167594614562 28.104026493708677 22.25912003039039 58-59 22.303103134272597 27.002682417312723 28.07651170612136 22.617702742293325 60-61 21.927516002824234 27.23786709791375 28.423066437061774 22.411550462200243 62-63 22.000066655992384 27.2634428938769 28.54573172081767 22.190758729313046 64-65 21.72856143007004 27.30236470384509 28.347872335218753 22.62120153086612 66-67 21.696879162059147 27.340442018692634 28.22471245014268 22.737966369105532 68-69 22.25362952907376 26.92776557691833 28.40248975228811 22.416115141719807 70-71 22.068299149267006 27.056683837937506 27.979616340487095 22.895400672308387 72-73 22.04866702117694 27.099249982406537 28.092989504973474 22.75909349144305 74-75 22.095996025063354 27.553773276661275 27.749933230572893 22.600297467702475 76-77 22.027116126970764 27.14034237647055 28.0924110223679 22.74013047419078 78-79 22.121405435063064 27.271833099050646 28.040991972545225 22.565769493341065 80-81 22.380576961125538 27.340553134894822 27.67778844851947 22.601081455460168 82-83 22.29059690397887 27.42214784921611 27.82652193300509 22.46073331379993 84-85 22.240859851167773 27.519721586919133 27.75069145292631 22.48872710898678 86-87 22.388017258720204 27.478738354081607 27.49533417384634 22.63791021335185 88-89 22.216491325304744 27.631746082059244 27.276316528546353 22.875446064089658 90-91 22.1962724649923 27.527316004178687 27.469138823897953 22.807272706931055 92-93 22.3473899373268 27.18152387478412 27.598910152872268 22.872176035016818 94-95 22.00607140543294 27.075364011658067 27.809656359198254 23.108908223710735 96-97 22.277367322768033 26.737874693945468 27.812870114488746 23.17188786879775 98-99 22.0893807619527 27.0186526468861 27.305922984423503 23.586043606737697 100-101 22.47587571706355 26.295584920645275 27.571524583837476 23.657014778453696 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 493.0 1 368.0 2 344.5 3 456.5 4 376.0 5 338.5 6 641.0 7 1464.5 8 2456.0 9 2777.5 10 2288.5 11 1792.5 12 1743.5 13 1889.5 14 2030.0 15 2198.5 16 2425.5 17 2643.5 18 2932.5 19 3356.5 20 3840.5 21 4574.0 22 5686.5 23 7107.0 24 8948.5 25 11427.0 26 14522.5 27 17924.5 28 20886.5 29 23785.5 30 26797.0 31 31430.5 32 38598.0 33 41828.5 34 42140.5 35 44553.0 36 48429.5 37 52736.0 38 56194.5 39 59076.5 40 62184.0 41 66137.0 42 68367.5 43 69677.0 44 71951.0 45 75555.0 46 77322.5 47 79353.5 48 81744.5 49 79198.5 50 75933.5 51 73895.5 52 72471.0 53 72564.0 54 72907.5 55 68786.0 56 62769.0 57 57439.5 58 53039.0 59 47077.0 60 37223.5 61 30350.0 62 26012.5 63 19350.0 64 13258.0 65 9835.5 66 7380.0 67 5549.0 68 4189.0 69 3134.5 70 2332.0 71 1613.0 72 1083.5 73 854.0 74 669.5 75 456.0 76 320.0 77 222.5 78 139.5 79 82.0 80 51.5 81 28.5 82 16.0 83 8.5 84 3.5 85 2.5 86 1.0 87 0.5 88 1.0 89 1.5 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.008481693057780834 2 0.012955553132214682 3 0.008854514730650322 4 4.6602709108685906E-5 5 7.456433457389745E-4 6 0.002003916491673494 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 6.524379275216027E-4 14-15 0.0 16-17 0.0026330530646407535 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.003541805892260129 26-27 0.018664384998028707 28-29 0.006477776566107341 30-31 0.002959272028401555 32-33 0.0 34-35 0.009087528276193753 36-37 7.689447002933175E-4 38-39 0.0026796557737494395 40-41 0.002027217846227837 42-43 0.002959272028401555 44-45 0.0 46-47 4.6602709108685906E-5 48-49 5.359311547498878E-4 50-51 0.004334051947107789 52-53 0.008668103894215578 54-55 0.007666145648378832 56-57 0.017848837588626702 58-59 0.02476933989126656 60-61 0.00386802485602093 62-63 0.021180931289897745 64-65 0.022206190890288836 66-67 0.014004114087160116 68-69 0.03192285573944985 70-71 0.01649735902447481 72-73 0.005359311547498879 74-75 0.016543961733583495 76-77 0.021879971926528033 78-79 0.02784511869243983 80-81 0.012279813850138736 82-83 0.035161744022503515 84-85 0.02313824507246255 86-87 0.031666540839352074 88-89 0.023604272163549413 90-91 0.029219898611146062 92-93 0.02530527104601645 94-95 0.015425496714975036 96-97 0.016171140060714007 98-99 0.012023498950040965 100-101 0.011277855604301988 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2145798.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.427910258263715 #Duplication Level Percentage of deduplicated Percentage of total 1 76.75323936342201 44.07778142193633 2 13.60023124542941 15.620657189083081 3 4.4290883741590825 7.630598690313807 4 1.822607529100919 4.186741664689734 5 0.9457886921284291 2.715733406741601 6 0.5747306153013882 1.9803346918922886 7 0.37268668308785263 1.4981832173573417 8 0.26473120928940336 1.2162368103706789 9 0.1834854689519673 0.9483468340202109 >10 0.9566898439892202 9.892721986149775 >50 0.061251921014284165 2.4161783348866543 >100 0.029546730561421285 3.3061588693507775 >500 0.003001725642367887 1.1603694011215244 >1k 0.0027583424821757963 2.6490116200500196 >5k 1.6225544012798802E-4 0.7009458620361699 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8179 0.38116355779994204 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6866 0.31997420074023747 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4451 0.207428658242761 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2977 0.13873626501655795 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2846 0.13263131012332008 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2714 0.12647975252097354 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2494 0.11622715651706265 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2474 0.11529510233488893 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2201 0.10257256274821767 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.6602709108685906E-5 0.0 5 0.0 0.0 0.0 4.6602709108685906E-5 0.0 6 0.0 0.0 0.0 4.6602709108685906E-5 0.0 7 0.0 0.0 0.0 4.6602709108685906E-5 0.0 8 0.0 0.0 0.0 4.6602709108685906E-5 0.0 9 0.0 0.0 0.0 4.6602709108685906E-5 0.0 10-11 0.0 0.0 0.0 4.6602709108685906E-5 0.0 12-13 0.0 0.0 0.0 4.6602709108685906E-5 4.6602709108685906E-5 14-15 0.0 0.0 0.0 4.6602709108685906E-5 6.990406366302886E-5 16-17 0.0 0.0 0.0 9.320541821737181E-5 9.320541821737181E-5 18-19 4.6602709108685906E-5 0.0 0.0 9.320541821737181E-5 1.8641083643474362E-4 20-21 9.320541821737181E-5 0.0 0.0 1.1650677277171475E-4 2.5631490009777247E-4 22-23 9.320541821737181E-5 0.0 0.0 1.8641083643474362E-4 2.796162546521154E-4 24-25 1.398081273260577E-4 0.0 0.0 3.7282167286948725E-4 2.796162546521154E-4 26-27 1.398081273260577E-4 0.0 0.0 8.621501185106892E-4 3.2621896376080133E-4 28-29 1.8641083643474362E-4 0.0 0.0 0.0024000395190973242 3.2621896376080133E-4 30-31 1.8641083643474362E-4 0.0 0.0 0.01018269194024787 3.495203183151443E-4 32-33 1.8641083643474362E-4 0.0 0.0 0.026773256382940053 3.7282167286948725E-4 34-35 1.8641083643474362E-4 0.0 0.0 0.05270766400192376 3.7282167286948725E-4 36-37 1.8641083643474362E-4 0.0 0.0 0.09367144530845867 3.7282167286948725E-4 38-39 2.3301354554342954E-4 0.0 0.0 0.15716763646904322 3.7282167286948725E-4 40-41 2.3301354554342954E-4 0.0 0.0 0.22392601726723577 3.7282167286948725E-4 42-43 2.3301354554342954E-4 0.0 0.0 0.29084750754730876 3.7282167286948725E-4 44-45 2.796162546521154E-4 0.0 0.0 0.37319449454235676 3.7282167286948725E-4 46-47 3.2621896376080133E-4 0.0 0.0 0.4678678980966522 3.7282167286948725E-4 48-49 3.2621896376080133E-4 0.0 0.0 0.5657801899340013 3.7282167286948725E-4 50-51 3.2621896376080133E-4 0.0 0.0 0.6724537910837833 3.7282167286948725E-4 52-53 3.2621896376080133E-4 0.0 0.0 0.7725564102492406 3.7282167286948725E-4 54-55 3.2621896376080133E-4 0.0 0.0 0.8798125452628813 3.7282167286948725E-4 56-57 3.2621896376080133E-4 0.0 0.0 1.0037058474283227 3.7282167286948725E-4 58-59 3.2621896376080133E-4 0.0 0.0 1.1355682128513496 3.7282167286948725E-4 60-61 3.2621896376080133E-4 0.0 0.0 1.2814579937160908 3.7282167286948725E-4 62-63 3.2621896376080133E-4 0.0 0.0 1.426672035298756 3.7282167286948725E-4 64-65 3.2621896376080133E-4 0.0 0.0 1.568740394016585 3.961230274238302E-4 66-67 3.2621896376080133E-4 0.0 0.0 1.7385140632995277 4.1942438197817316E-4 68-69 3.2621896376080133E-4 0.0 0.0 1.9174451649223272 4.1942438197817316E-4 70-71 3.2621896376080133E-4 0.0 0.0 2.106046328685179 4.1942438197817316E-4 72-73 3.7282167286948725E-4 0.0 0.0 2.2988417362678124 4.66027091086859E-4 74-75 4.1942438197817316E-4 0.0 0.0 2.4870234756486864 5.126298001955449E-4 76-77 4.1942438197817316E-4 0.0 0.0 2.686995700434058 5.126298001955449E-4 78-79 4.1942438197817316E-4 0.0 0.0 2.9147431398482055 5.126298001955449E-4 80-81 4.1942438197817316E-4 0.0 0.0 3.155166516139916 5.359311547498879E-4 82-83 4.1942438197817316E-4 0.0 0.0 3.4033259421436686 6.058352184129168E-4 84-85 4.1942438197817316E-4 0.0 0.0 3.6593845273413432 6.058352184129168E-4 86-87 4.427257365325161E-4 0.0 0.0 3.9209189308592887 6.291365729672598E-4 88-89 4.6602709108685907E-4 0.0 0.0 4.195758407827764 6.524379275216027E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5215 0.0 41.4523 1 GTATCAA 8255 0.0 35.222965 1 ATCAACG 9955 0.0 28.912876 3 TCAACGC 10070 0.0 28.580027 4 TATCAAC 10225 0.0 28.340498 2 CAACGCA 10150 0.0 28.261183 5 AACGCAG 10550 0.0 27.144653 6 GTACATG 6345 0.0 24.934702 1 TAAGGTG 1660 0.0 24.604233 5 TACATGG 6150 0.0 24.486406 2 ACGCAGA 11730 0.0 24.130016 7 CGCAGAG 11790 0.0 23.926653 8 ACATGGG 6205 0.0 23.422842 3 GTAAGGT 1715 0.0 22.984415 4 GAGTACT 8035 0.0 22.666729 12-13 GGTAAGG 1635 0.0 22.077799 3 GCAGAGT 12905 0.0 21.491373 9 GTACTTT 8360 0.0 21.33059 14-15 GTATAGG 580 0.0 21.297905 1 AGGTAAG 1615 0.0 21.178778 2 >>END_MODULE