##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937991_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 57685 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.10501863569385 33.0 31.0 34.0 30.0 34.0 2 32.2927970876311 33.0 31.0 34.0 30.0 34.0 3 32.24401490855509 34.0 31.0 34.0 30.0 34.0 4 35.84597382335096 37.0 35.0 37.0 35.0 37.0 5 35.79932391436249 37.0 35.0 37.0 35.0 37.0 6 35.96187917136171 37.0 35.0 37.0 35.0 37.0 7 35.9040478460605 37.0 35.0 37.0 35.0 37.0 8 35.983808615757994 37.0 35.0 37.0 35.0 37.0 9 37.47308659096819 39.0 37.0 39.0 34.0 39.0 10-11 37.53419433128197 39.0 37.0 39.0 35.0 39.0 12-13 37.44070382248418 39.0 37.0 39.0 34.5 39.0 14-15 38.29239837046026 40.0 37.5 41.0 33.0 41.0 16-17 38.15003033717605 40.0 37.5 41.0 33.5 41.0 18-19 38.45231862702609 40.0 37.5 41.0 34.0 41.0 20-21 38.56756522492849 40.0 37.5 41.0 35.0 41.0 22-23 38.3886972349831 40.0 37.0 41.0 34.0 41.0 24-25 38.134775071509054 40.0 36.5 41.0 34.0 41.0 26-27 37.740123082257085 40.0 35.5 41.0 33.5 41.0 28-29 37.39321314033111 39.0 35.0 41.0 33.0 41.0 30-31 36.699315246597905 38.5 35.0 40.5 33.0 41.0 32-33 35.86581433648262 36.5 35.0 40.0 31.5 41.0 34-35 35.190413452370635 35.5 35.0 40.0 30.5 41.0 36-37 34.69577879864783 35.0 35.0 40.0 29.0 41.0 38-39 34.04808009014475 35.0 35.0 40.0 25.0 41.0 40-41 33.618904394556644 35.0 34.5 40.0 22.0 41.0 42-43 32.905538701568865 35.0 33.5 40.0 10.0 41.0 44-45 31.997156973216608 35.0 33.0 40.0 2.0 41.0 46-47 30.806700182023057 35.0 32.5 40.0 2.0 41.0 48-49 29.73442836092572 35.0 30.5 39.0 2.0 41.0 50-51 28.657935338476207 35.0 26.5 38.5 2.0 41.0 52-53 27.829435728525613 35.0 21.5 38.0 2.0 40.0 54-55 27.122562191210886 35.0 7.5 38.0 2.0 40.0 56-57 26.108936465285602 34.5 2.0 38.0 2.0 40.0 58-59 25.01951113807749 34.0 2.0 37.0 2.0 40.0 60-61 23.761775158186705 33.0 2.0 36.0 2.0 40.0 62-63 22.655525699921988 32.0 2.0 36.0 2.0 39.5 64-65 21.5471613070989 31.0 2.0 35.0 2.0 39.0 66-67 20.352925370546934 29.5 2.0 35.0 2.0 38.5 68-69 19.28270780965589 26.5 2.0 35.0 2.0 37.5 70-71 18.442992112334228 22.5 2.0 35.0 2.0 37.0 72-73 17.88147698708503 17.0 2.0 35.0 2.0 36.0 74-75 17.470364912888968 8.5 2.0 34.5 2.0 35.0 76-77 16.60529600416053 2.0 2.0 32.5 2.0 35.0 78-79 16.958074022709543 2.0 2.0 34.0 2.0 35.0 80-81 16.773918696368206 2.0 2.0 34.0 2.0 35.0 82-83 16.500927450810437 2.0 2.0 34.0 2.0 35.0 84-85 16.161801161480454 2.0 2.0 34.0 2.0 35.0 86-87 15.862919303111727 2.0 2.0 33.0 2.0 35.0 88-89 15.467937938805582 2.0 2.0 33.0 2.0 35.0 90-91 15.162017855595042 2.0 2.0 33.0 2.0 35.0 92-93 14.702175608910462 2.0 2.0 33.0 2.0 35.0 94-95 14.283383895293404 2.0 2.0 32.5 2.0 35.0 96-97 13.769576146311866 2.0 2.0 32.0 2.0 35.0 98-99 13.091852301291496 2.0 2.0 31.0 2.0 35.0 100-101 11.051694547976076 2.0 2.0 27.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1513014462381932 1101 2 0.2277663547316635 1101 3 0.7017744927168579 1101 4 0.08121924610657771 1101 5 -0.4734734431253429 1101 6 -0.33240207403335376 1101 7 -0.11620948948991838 1101 8 -0.6839466487691297 1101 9 -0.3749828963907973 1101 10-11 0.5540724570988687 1101 12-13 0.6621271891926099 1101 14-15 0.5318165591567805 1101 16-17 0.5851899807646461 1101 18-19 0.8637743711360528 1101 20-21 0.997774561740087 1101 22-23 1.037254843644206 1101 24-25 1.2918066041608611 1101 26-27 1.2664211352991543 1101 28-29 0.4360339838525391 1101 30-31 0.8352169817169965 1101 32-33 1.1935515505696515 1101 34-35 1.8452323077235206 1101 36-37 2.021613637911102 1101 38-39 1.5093821295784124 1101 40-41 1.0378258185299387 1101 42-43 0.6079256399472186 1101 44-45 -0.49535240007308445 1101 46-47 -0.5604855948071652 1101 48-49 -0.5089332702082636 1101 50-51 0.018530402854544548 1101 52-53 -0.5122654306909453 1101 54-55 -1.5920254015325987 1101 56-57 -2.1418520195533794 1101 58-59 -0.9632127473730385 1101 60-61 -1.4527137104764094 1101 62-63 -1.1086266246882879 1101 64-65 -0.17525165154846079 1101 66-67 1.0135514700162496 1101 68-69 1.1218445665299868 1101 70-71 2.026450469934584 1101 72-73 2.3012552130849926 1101 74-75 2.540601599155977 1101 76-77 2.4286675837631826 1101 78-79 2.5971610646534913 1101 80-81 2.617557450918085 1101 82-83 2.6594336540279677 1101 84-85 2.703271288526743 1101 86-87 2.6918884574157538 1101 88-89 2.977515350599724 1101 90-91 3.2611308051656476 1101 92-93 2.7893715497266083 1101 94-95 2.954372025925359 1101 96-97 2.6196603041132303 1101 98-99 3.0509240605618384 1101 100-101 4.502202112907447 1102 1 0.2510430483053767 1102 2 0.47169400331047484 1102 3 -0.40520225146918776 1102 4 -0.0035352758572457788 1102 5 -0.6667550968721088 1102 6 -0.3814976812684918 1102 7 -0.1735738305751866 1102 8 0.05817221427996344 1102 9 0.3774718839709621 1102 10-11 0.08223783177587052 1102 12-13 0.08925897213834588 1102 14-15 0.15274679171491812 1102 16-17 -0.44581777117333843 1102 18-19 0.13069943573552933 1102 20-21 0.1326582826703131 1102 22-23 0.02226776354084592 1102 24-25 0.5414190072616449 1102 26-27 0.4038888355575594 1102 28-29 0.625181270674247 1102 30-31 0.6029172401149268 1102 32-33 -0.4635543925828003 1102 34-35 0.6315372172842473 1102 36-37 0.7112777205984386 1102 38-39 0.9654544809995969 1102 40-41 1.1856294361009958 1102 42-43 1.484669825993734 1102 44-45 1.3369473415289832 1102 46-47 1.2069562656579542 1102 48-49 0.6954594946496151 1102 50-51 1.0451453899346461 1102 52-53 1.6879929672418683 1102 54-55 0.9847187845139125 1102 56-57 1.7718430708858968 1102 58-59 1.498027562704479 1102 60-61 2.6294568321592493 1102 62-63 2.395507742236777 1102 64-65 0.8689343949631692 1102 66-67 1.8869364829361466 1102 68-69 2.635022861103632 1102 70-71 3.3926003407356227 1102 72-73 2.988335316444168 1102 74-75 3.5863380332645036 1102 76-77 3.218331666450524 1102 78-79 2.576230832095355 1102 80-81 2.637712489677778 1102 82-83 2.1454801656558757 1102 84-85 2.85934363994793 1102 86-87 3.194214038811104 1102 88-89 2.529970130961484 1102 90-91 2.596531321961516 1102 92-93 1.8206376995974072 1102 94-95 1.907085204219932 1102 96-97 1.4333553945525086 1102 98-99 1.5558336212853554 1102 100-101 2.2435457821580957 1103 1 -0.0817852345229042 1103 2 -0.1474979158814449 1103 3 -0.04611134237828196 1103 4 0.19696977464780474 1103 5 -0.23140156151857383 1103 6 0.15385585408504454 1103 7 0.07036556336420574 1103 8 -0.5089995028917542 1103 9 -0.7629321564330809 1103 10-11 -0.06220661266857519 1103 12-13 -0.7501349672555904 1103 14-15 -1.1108895719214402 1103 16-17 -0.07839352874909622 1103 18-19 0.13095196098806383 1103 20-21 -0.11506899005695459 1103 22-23 0.29095463222771656 1103 24-25 0.5899038557464849 1103 26-27 0.6889898456585613 1103 28-29 0.5441206646136365 1103 30-31 1.0175637047613932 1103 32-33 0.2836678296394197 1103 34-35 -0.5310890453420214 1103 36-37 -0.676853592532872 1103 38-39 -1.3085354179902993 1103 40-41 -1.0002291497575868 1103 42-43 -1.449421083097171 1103 44-45 -2.7345173049356646 1103 46-47 -2.1453164616147724 1103 48-49 -2.387368788178666 1103 50-51 -2.689450569661311 1103 52-53 -2.2678151135662112 1103 54-55 -1.183463033667909 1103 56-57 -2.288257939215111 1103 58-59 -1.7103057706288531 1103 60-61 -0.6834219557195382 1103 62-63 -0.02696700523797091 1103 64-65 -0.48788378685501144 1103 66-67 0.1192597152593784 1103 68-69 -1.1952801691993997 1103 70-71 -1.3829047097694307 1103 72-73 -0.8530788249699732 1103 74-75 -0.5098740879476154 1103 76-77 0.3552003533192085 1103 78-79 0.7512308320953522 1103 80-81 0.4915003684656547 1103 82-83 1.402298347474055 1103 84-85 1.4194446500489377 1103 86-87 1.2669413115383783 1103 88-89 2.0554751814665337 1103 90-91 1.7280969785271711 1103 92-93 1.7428599218196315 1103 94-95 2.3563276284623598 1103 96-97 2.2687089299060457 1103 98-99 1.479570995022728 1103 100-101 2.557434671046984 1104 1 -0.006473291563899863 1104 2 -0.12699880714703937 1104 3 -0.028731663233891425 1104 4 -0.47281632160888165 1104 5 -0.390938103408061 1104 6 -0.09391598192208761 1104 7 0.4656418556993245 1104 8 -0.00588007330173923 1104 9 0.7513280931212876 1104 10-11 -0.07201053423720793 1104 12-13 -0.3793684788420393 1104 14-15 0.21353110544040987 1104 16-17 -0.014445222153732118 1104 18-19 0.39409812854599124 1104 20-21 0.4444229885526667 1104 22-23 0.40592789425979703 1104 24-25 0.7414190072616336 1104 26-27 1.271209096995463 1104 28-29 0.5938087216546393 1104 30-31 0.19180612900381533 1104 32-33 1.031216849247265 1104 34-35 1.6106221846045017 1104 36-37 1.5439574591605307 1104 38-39 1.932774742437509 1104 40-41 2.8830150570160242 1104 42-43 2.8003561005035422 1104 44-45 3.510803550679313 1104 46-47 2.834407246050109 1104 48-49 2.153629429290138 1104 50-51 2.514426435686282 1104 52-53 2.8611955816209544 1104 54-55 2.4533462354943048 1104 56-57 2.0829541819970068 1104 58-59 1.5372432489789922 1104 60-61 1.8372999694141505 1104 62-63 2.2785142781844883 1104 64-65 2.9218755714337554 1104 66-67 3.086282888164906 1104 68-69 2.8546307042408863 1104 70-71 3.191293151193136 1104 72-73 3.539969303372274 1104 74-75 3.0451615626762703 1104 76-77 2.7647368952086957 1104 78-79 3.4546622046443716 1104 80-81 3.698496803403266 1104 82-83 4.502342910753914 1104 84-85 4.205095273934859 1104 86-87 4.86676305841895 1104 88-89 3.692061634229457 1104 90-91 4.621367923268702 1104 92-93 5.027173647309825 1104 94-95 3.532575400298363 1104 96-97 3.550348858604794 1104 98-99 4.3231538827232665 1104 100-101 4.1579248671254145 1105 1 -0.02608113470115825 1105 2 -0.36229292479409736 1105 3 -0.7150061730378177 1105 4 -0.9826202431775073 1105 5 -0.4105459465453123 1105 6 -0.6429355897652229 1105 7 -0.49514245802616585 1105 8 -0.3588212497723262 1105 9 -0.8172993578591061 1105 10-11 -0.9445595538450462 1105 12-13 -0.62446651805773 1105 14-15 -0.4923512475007712 1105 16-17 -0.4458177711733313 1105 18-19 -0.14511755772851842 1105 20-21 0.013050439533067504 1105 22-23 -1.0548564194656862 1105 24-25 -0.18995354175796564 1105 26-27 -0.48369286378885334 1105 28-29 -0.6708971606983027 1105 30-31 -1.0140762239373586 1105 32-33 -1.204077268399793 1105 34-35 -0.8893778153954912 1105 36-37 -1.1913366584865273 1105 38-39 -1.890754669327201 1105 40-41 -2.3228672959251497 1105 42-43 -1.7486635073396002 1105 44-45 -2.871549390497158 1105 46-47 -3.518533930420478 1105 48-49 -3.4444097863961325 1105 50-51 -3.7110637603921504 1105 52-53 -3.9231181438692424 1105 54-55 -5.860379254701776 1105 56-57 -5.328810523885341 1105 58-59 -4.1784430255308145 1105 60-61 -3.3783863050956526 1105 62-63 -4.260701408090018 1105 64-65 -4.303614624644677 1105 66-67 -3.9039131902664685 1105 68-69 -4.429683021249311 1105 70-71 -3.789099005669609 1105 72-73 -3.607089520157139 1105 74-75 -3.3960149079119653 1105 76-77 -3.539184673418756 1105 78-79 -4.123769167904648 1105 80-81 -3.1250326083614404 1105 82-83 -3.3505982657166733 1105 84-85 -2.9027478633200445 1105 86-87 -2.7018643925614434 1105 88-89 -3.268722679496033 1105 90-91 -3.1629458022214934 1105 92-93 -3.217924391905859 1105 94-95 -2.957620678133008 1105 96-97 -1.9594550629638317 1105 98-99 -1.765081411394382 1105 100-101 -1.8518790544432129 1106 1 0.03583836994280176 1106 2 -0.10842295575385208 1106 3 0.21585038010975666 1106 4 0.22687408086790128 1106 5 0.17768934757233268 1106 6 -0.05986025436791209 1106 7 -0.327959795487466 1106 8 0.2067102259758684 1106 9 0.028933872275054284 1106 10-11 -0.14115398108963007 1106 12-13 -0.08267085242305683 1106 14-15 0.515904686451762 1106 16-17 0.15067345689683975 1106 18-19 0.04064095620336872 1106 20-21 0.40985126512645564 1106 22-23 -0.2297790200848837 1106 24-25 -0.8989318699313458 1106 26-27 -1.7721345562553168 1106 28-29 -0.8958713609047066 1106 30-31 -0.13275527450495161 1106 32-33 -0.11326199492198441 1106 34-35 -0.4146616131251122 1106 36-37 -0.41269888758868944 1106 38-39 -0.7596917178308047 1106 40-41 -0.9260664703317545 1106 42-43 -1.292523156462405 1106 44-45 -0.9045731263072732 1106 46-47 -0.7842718045174841 1106 48-49 -0.1425522012568159 1106 50-51 -0.2905271246852337 1106 52-53 -0.7213637579043279 1106 54-55 0.06892931082969866 1106 56-57 0.5215506732250823 1106 58-59 1.3664486153360578 1106 60-61 1.4750708672469663 1106 62-63 1.6598352276168917 1106 64-65 2.2092852721561513 1106 66-67 2.419100225626206 1106 68-69 2.0630930365422238 1106 70-71 1.883243615589425 1106 72-73 2.9228382404207736 1106 74-75 3.933706454317136 1106 76-77 4.073302426684442 1106 78-79 3.446406270691842 1106 80-81 3.0885896826602313 1106 82-83 3.240217007761135 1106 84-85 3.6447237569069912 1106 86-87 2.7047403546005775 1106 88-89 2.258625101721716 1106 90-91 2.816414362897186 1106 92-93 2.277947641117878 1106 94-95 2.5000676603602834 1106 96-97 3.3356945758390584 1106 98-99 1.8148979487707368 1106 100-101 2.2482241447311946 1107 1 0.2045784018407346 1107 2 0.2797748113912846 1107 3 0.6493432030762634 1107 4 0.19242432010234722 1107 5 0.6322348021177859 1107 6 0.5902194904486748 1107 7 0.38400192700056834 1107 8 0.4319095880173407 1107 9 0.9552496617487378 1107 10-11 0.401429750967786 1107 12-13 0.513501396380768 1107 14-15 0.8891104280785598 1107 16-17 1.1693337439781786 1107 18-19 0.7468610518971488 1107 20-21 0.43265828267032447 1107 22-23 0.16368190495499135 1107 24-25 0.46263112847375965 1107 26-27 0.859444391113108 1107 28-29 0.9191206646136436 1107 30-31 0.42210915930684934 1107 32-33 1.0404860114576024 1107 34-35 0.8325473182943455 1107 36-37 1.1617827711034963 1107 38-39 1.866464582009698 1107 40-41 2.7724981229696795 1107 42-43 3.002851644175557 1107 44-45 3.8450281496097887 1107 46-47 4.625138083839776 1107 48-49 5.485358484548602 1107 50-51 5.194640339429597 1107 52-53 4.989003068251975 1107 54-55 3.893809693604819 1107 56-57 3.9071966062394345 1107 58-59 4.6374215020984195 1107 60-61 4.932487135189554 1107 62-63 4.6207602674893025 1107 64-65 3.9871162131449864 1107 66-67 4.1010778970775625 1107 68-69 4.550174376255146 1107 70-71 5.214822562957842 1107 72-73 4.965102993211843 1107 74-75 4.90148954841602 1107 76-77 5.02338217150103 1107 78-79 5.0035035593680774 1107 80-81 4.589227641192924 1107 82-83 4.300025620201328 1107 84-85 4.451262831867119 1107 86-87 4.812395856992925 1107 88-89 4.33502063601199 1107 90-91 2.57127879670899 1107 92-93 3.0610417400014516 1107 94-95 3.5586003557350843 1107 96-97 3.59598165717877 1107 98-99 2.765934631386365 1107 100-101 1.9960710346833483 1108 1 0.14548749274982242 1108 2 0.36998460160107527 1108 3 0.16018236391542473 1108 4 0.36655019422822477 1108 5 -0.03384911396612722 1108 6 0.38986984009903125 1108 7 0.2084774514760923 1108 8 0.03295853906628565 1108 9 0.728326584825659 1108 10-11 0.428178002716038 1108 12-13 0.6379769208562962 1108 14-15 1.0315929455610728 1108 16-17 0.5125155621599973 1108 18-19 -0.5438732138371094 1108 20-21 -0.6692647942527614 1108 22-23 -0.09803138175829673 1108 24-25 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3.0015658621701533 2316 86-87 2.8808807054777716 2316 88-89 2.9810812420725945 2316 90-91 2.947642433072625 2316 92-93 3.5628599218196317 2316 94-95 3.8470852042199333 2316 96-97 3.551133172330287 2316 98-99 3.504722510174245 2316 100-101 3.965768004380317 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 2.0 5 12.0 6 20.0 7 46.0 8 76.0 9 147.0 10 292.0 11 503.0 12 708.0 13 1047.0 14 1221.0 15 1665.0 16 2151.0 17 2734.0 18 2588.0 19 2019.0 20 2031.0 21 2133.0 22 2159.0 23 2214.0 24 2296.0 25 2074.0 26 1507.0 27 864.0 28 899.0 29 1046.0 30 1117.0 31 1259.0 32 1520.0 33 1980.0 34 2849.0 35 5289.0 36 4374.0 37 5123.0 38 1661.0 39 59.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.96368206639508 23.4896420213227 14.873884025309872 14.67279188697235 2 15.020804438280166 26.30721220527046 39.207697642163666 19.464285714285715 3 21.446526706308617 35.877121508936774 33.79851949447844 8.877832290276165 4 10.025136517292191 27.45254398890526 30.170754962295227 32.35156453150733 5 6.0969471875596595 44.66755757649386 33.174820805637026 16.060674430309447 6 18.205772731212623 40.89624685793534 13.55118314986565 27.346797260986396 7 12.55439022276155 46.03449770304239 18.885325474560112 22.525786599635957 8 23.44456964548843 32.82482447776719 19.311779492068997 24.418826384675395 9 21.163214007107566 20.911848834185665 19.772904567911937 38.15203259079483 10-11 21.017595562104532 31.339169628152902 21.827164774204732 25.816070035537837 12-13 28.54034844413626 30.00520065875011 24.069515471959782 17.384935425153852 14-15 24.179596082170406 30.30423853688134 27.632833492242348 17.883331888705904 16-17 14.819277108433734 37.04429227702175 27.963075322874232 20.173355291670276 18-19 14.066048366126378 31.515125249198235 35.46069168761377 18.958134697061627 20-21 16.64904221201352 29.020542602062925 34.60171621738753 19.728698968536015 22-23 14.65979024009708 33.31715350611078 35.273467972609865 16.749588281182284 24-25 16.74178729305712 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19.317846927277454 50-51 10.42992112334229 29.75816936811996 41.13981104273208 18.67209846580567 52-53 11.802895033370895 30.48019415792667 40.63794747334663 17.078963335355812 54-55 11.011528126896074 29.261506457484614 37.88853254745601 21.838432868163302 56-57 14.913755742394036 30.455057640634482 33.636127242784085 20.9950593741874 58-59 12.879431394643321 32.73381294964029 36.28846320533935 18.09829245037705 60-61 13.477507150905781 36.425413885758864 31.5888012481581 18.508277715177258 62-63 10.709023142931438 38.49007540955188 30.919649822310824 19.88125162520586 64-65 10.967322527520153 38.705035971223026 30.33197538354858 19.995666117708243 66-67 11.709283175868944 36.05096645575106 29.92459044812343 22.315159920256566 68-69 11.62693941232556 33.99930657883332 31.255092311692817 23.118661697148305 70-71 13.416832798821185 31.85576839733033 30.888445869810177 23.838952934038314 72-73 12.820490595475425 29.05954754268874 32.89676692381035 25.223194938025483 74-75 12.665337609430527 29.162693941232554 31.319233769610815 26.852734679726098 76-77 12.648868856721851 26.84753402097599 33.97330328508278 26.530293837219382 78-79 12.875964288809916 26.493022449510274 34.24373754008841 26.3872757215914 80-81 13.372627199445263 25.985958221374705 33.27814856548496 27.36326601369507 82-83 12.732079396723586 25.808269047412676 33.672531854034844 27.7871197018289 84-85 13.192795104276822 25.961721824454347 32.48530762963092 28.36017544163792 86-87 13.254975383121836 25.72377088967478 31.856320643506 29.164933083697385 88-89 13.468839386322268 25.331541995319405 30.020802635000432 31.178815983357893 90-91 13.882652751653419 23.90198236930838 31.514211169571887 30.70115370946631 92-93 14.007974343416832 24.664990898847186 30.82343763543382 30.503597122302157 94-95 13.759209499869984 23.681199618618358 30.844240270434252 31.715350611077405 96-97 12.607263586720984 24.415359278841986 31.00459391522926 31.972783219207766 98-99 13.77654502903701 24.734333015515297 29.346450550403052 32.14267140504464 100-101 13.453237410071942 23.735806535494497 29.56921210019936 33.2417439542342 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1869.0 1 1063.5 2 342.0 3 422.0 4 286.5 5 198.0 6 389.5 7 616.5 8 776.0 9 849.5 10 621.0 11 329.5 12 256.5 13 262.5 14 281.5 15 283.0 16 277.5 17 304.0 18 326.5 19 346.5 20 391.0 21 443.0 22 485.0 23 554.5 24 636.0 25 696.5 26 760.5 27 842.0 28 906.0 29 1002.5 30 1108.5 31 1163.5 32 1235.0 33 1293.0 34 1312.0 35 1277.0 36 1242.5 37 1197.5 38 1106.0 39 1024.0 40 971.0 41 985.5 42 1026.0 43 1079.0 44 1142.0 45 1214.5 46 1386.0 47 2047.5 48 3397.0 49 3572.5 50 3043.0 51 3175.0 52 2865.5 53 1774.5 54 732.0 55 344.0 56 211.5 57 169.5 58 149.0 59 122.5 60 103.0 61 75.5 62 51.5 63 45.5 64 34.0 65 22.5 66 19.5 67 19.5 68 19.0 69 12.0 70 6.5 71 7.0 72 7.0 73 5.0 74 3.0 75 1.0 76 0.5 77 0.5 78 1.0 79 0.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.008667764583513912 3 0.0034671058334055645 4 0.0 5 0.11441449250238363 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 8.667764583513911E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.006067435208459738 36-37 8.667764583513911E-4 38-39 0.004333882291756956 40-41 0.006934211666811129 42-43 0.004333882291756956 44-45 0.0026003293750541737 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0034671058334055645 86-87 0.0017335529167027822 88-89 0.0 90-91 0.0026003293750541737 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 57685.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.47620698621825 #Duplication Level Percentage of deduplicated Percentage of total 1 85.74904257715701 32.99297911068735 2 7.285424645190358 5.606310132616798 3 2.1220995719756703 2.4495102713010315 4 1.1804460464068485 1.8167634567045159 5 0.6893444469475106 1.3261679812776286 6 0.4911015994593377 1.1337436075236196 7 0.39198017571525123 1.0557337262719946 8 0.23879252083802657 0.7350264366819798 9 0.21175940527145753 0.7332928837652769 >10 1.306600585717504 10.000866776458352 >50 0.12164902004956071 3.2365432954840947 >100 0.16219869339941428 12.500650082343764 >500 0.022527596305474205 5.951287163040652 >1k 0.02703311556656905 20.46112507584294 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT 2624 4.5488428534281 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2237 3.8779578746641246 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2116 3.6681979717430875 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1990 3.449770304238537 No Hit ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT 1471 2.550056340469793 RNA PCR Primer, Index 31 (95% over 22bp) TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1365 2.366299731299298 No Hit ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT 882 1.528993672531854 RNA PCR Primer, Index 31 (95% over 24bp) GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 758 1.314033110860709 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 710 1.2308225708589755 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 561 0.972523186270261 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 522 0.9049146225188524 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 446 0.7731646008494409 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 398 0.6899540608477074 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 381 0.66048366126376 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 341 0.5911415445956487 No Hit CATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCT 340 0.5894079916789461 RNA PCR Primer, Index 31 (96% over 27bp) TTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC 340 0.5894079916789461 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 257 0.44552309959261505 No Hit CACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTG 257 0.44552309959261505 RNA PCR Primer, Index 38 (95% over 24bp) GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 252 0.4368553350091012 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 248 0.42992112334229005 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 219 0.37964808875790934 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 218 0.37791453584120654 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 196 0.33977637167374536 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 192 0.33284216000693423 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 183 0.3172401837566092 No Hit TATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCT 181 0.31377307792320364 RNA PCR Primer, Index 31 (95% over 21bp) GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 171 0.2964375487561758 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 170 0.29470399583947304 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 168 0.29123689000606745 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 165 0.2860362312559591 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 152 0.26350004333882293 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 149 0.25829938458871454 No Hit CTTTACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC 145 0.25136517292190347 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 144 0.24963162000520067 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 143 0.24789806708849788 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 136 0.2357631966715784 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 131 0.22709543208806449 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 129 0.22362832625465895 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 127 0.22016122042125336 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 126 0.2184276675045506 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 125 0.21669411458784782 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 125 0.21669411458784782 No Hit CTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC 121 0.20975990292103666 No Hit ACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGC 119 0.20629279708763112 RNA PCR Primer, Index 38 (96% over 25bp) AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 112 0.1941579266707116 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 104 0.18028950333708937 No Hit GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACGCTACGC 97 0.16815463292016988 No Hit GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 89 0.15428620958654762 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 89 0.15428620958654762 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 88 0.15255265666984485 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 88 0.15255265666984485 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 83 0.14388489208633093 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 82 0.14215133916962813 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 82 0.14215133916962813 No Hit GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 75 0.13001646875270867 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 73 0.1265493629193031 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 72 0.12481581000260034 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 69 0.11961515125249197 No Hit GGTATCAACGCAGAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 67 0.11614804541908642 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 65 0.11268093958568086 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 63 0.1092138337522753 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 63 0.1092138337522753 No Hit GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA 63 0.1092138337522753 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 62 0.10748028083557251 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 61 0.10574672791886972 No Hit GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA 60 0.10401317500216695 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 58 0.10054606916876138 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0017335529167027824 0.0 16-17 0.0 0.0 0.0 0.0017335529167027824 0.0 18-19 0.0 0.0 0.0 0.005200658750108347 0.0 20-21 0.0 0.0 0.0 0.01473519979197365 0.0 22-23 0.0 0.0 0.0 0.039871717084163996 0.0 24-25 0.0 0.0 0.0 0.09187830458524747 0.0 26-27 0.0 0.0 0.0 0.21496056167114502 0.0 28-29 0.0 0.0 0.0 0.5139984398023749 0.0 30-31 0.0 0.0 0.0 1.0973389962728612 0.0 32-33 0.0 0.0 0.0 1.755222328161567 0.0 34-35 0.0 0.0 0.0 2.7858195371413714 0.0 36-37 0.0 0.0 0.0 4.014908555083644 0.0 38-39 0.0 0.0 0.0 5.232729479067348 0.0 40-41 0.0 0.0 0.0 6.40547802721678 0.0 42-43 0.0 0.0 0.0 7.369333448903528 0.0 44-45 0.0 0.0 0.0 8.131229955794401 0.0 46-47 0.0 0.0 0.0 8.862789286642975 0.0 48-49 0.0 0.0 0.0 9.57094565311606 0.0 50-51 0.0 0.0 0.0 10.1872237150039 0.0 52-53 0.0 0.0 0.0 10.639681026263327 0.0 54-55 0.0 0.0 0.0 11.20828638294184 0.0 56-57 0.0 0.0 0.0 11.841899973996707 0.0 58-59 0.0 0.0 0.0 12.271821097338997 0.0 60-61 0.0 0.0 0.0 12.702608997139638 0.0 62-63 0.0 0.0 0.0 13.116928144231602 0.0 64-65 0.0 0.0 0.0 13.438502210279967 0.0 66-67 0.0 0.0 0.0 13.781745687787119 0.0 68-69 0.0 0.0 0.0 14.235936551963249 0.0 70-71 0.0 0.0 0.0 14.695328074889487 0.0 72-73 0.0 0.0 0.0 15.069775504897287 0.0 74-75 0.0 0.0 0.0 15.447690040738493 0.0 76-77 0.0 0.0 0.0 15.736326601369507 0.0 78-79 0.0 0.0 0.0 16.145445089711362 0.0 80-81 0.0 0.0 0.0 16.51642541388576 0.0 82-83 0.0 0.0 0.0 16.7920603276415 0.0 84-85 0.0 0.0 0.0 17.114501170148216 0.0 86-87 0.0 0.0 0.0 17.404871283695933 0.0 88-89 0.0 0.0 0.0 17.716910808702437 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATAAG 35 3.3787728E-6 67.847855 8 TGTATAA 35 3.3787728E-6 67.847855 7 GTACCCA 60 3.1468517E-10 63.434696 1 GTGTATA 40 7.461347E-6 59.36687 6 TATAAGA 40 7.461347E-6 59.36687 9 ACCCATG 65 6.4210326E-10 58.5551 3 AGATGTG 45 1.4837717E-5 52.862247 2 GAGATGT 45 1.4837717E-5 52.862247 1 ATGTGTA 45 1.49143525E-5 52.81636 4 GACAGAC 25 2.81335E-5 47.493496 16-17 TACCCAT 85 6.9194357E-9 44.77743 2 GATGTGT 55 4.8584618E-5 43.250927 3 TGTGTAT 55 4.883479E-5 43.213383 5 ATGGGGG 55 4.9086033E-5 43.175903 7 AGACAGA 30 8.251057E-5 39.577915 14-15 ATAAGAG 35 2.0435789E-4 33.923927 10-11 GACACAT 35 2.0435789E-4 33.923927 20-21 TGGGGCA 60 0.0039227996 31.66233 6 GCAGAAA 90 2.375955E-5 31.66233 9 CATGGGG 225 0.0 31.662329 6 >>END_MODULE