##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937990_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 57627 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.12811702847623 33.0 31.0 34.0 30.0 34.0 2 32.337307165044166 33.0 31.0 34.0 30.0 34.0 3 32.157617089211655 34.0 31.0 34.0 30.0 34.0 4 35.83063494542489 37.0 35.0 37.0 35.0 37.0 5 35.83023582695611 37.0 35.0 37.0 35.0 37.0 6 35.99062939247228 37.0 35.0 37.0 35.0 37.0 7 35.94179811546671 37.0 35.0 37.0 35.0 37.0 8 35.997865583840905 37.0 35.0 37.0 35.0 37.0 9 37.524962257275234 39.0 37.0 39.0 35.0 39.0 10-11 37.556527322262134 39.0 37.0 39.0 35.0 39.0 12-13 37.490282332934214 39.0 37.0 39.0 35.0 39.0 14-15 38.354070140732645 40.0 38.0 41.0 33.5 41.0 16-17 38.25381331667448 40.0 37.5 41.0 33.5 41.0 18-19 38.3731497388377 40.0 37.5 41.0 34.0 41.0 20-21 38.52284519409305 40.0 37.5 41.0 34.5 41.0 22-23 38.39592552102313 40.0 37.0 41.0 34.5 41.0 24-25 38.17702639387787 40.0 36.5 41.0 34.0 41.0 26-27 37.78986412619085 40.0 35.5 41.0 33.5 41.0 28-29 37.462717129123504 39.5 35.0 41.0 33.0 41.0 30-31 36.769578496191016 38.5 35.0 40.5 33.0 41.0 32-33 35.925980877019455 36.5 35.0 40.0 32.0 41.0 34-35 35.285048675100214 35.5 35.0 40.0 31.0 41.0 36-37 34.714465441546494 35.0 35.0 40.0 29.0 41.0 38-39 34.1254359935447 35.0 35.0 40.0 25.5 41.0 40-41 33.76472833914658 35.0 35.0 40.0 23.0 41.0 42-43 33.06358130737328 35.0 33.5 40.0 12.0 41.0 44-45 32.25298037378312 35.0 33.0 40.0 2.0 41.0 46-47 31.168193728634147 35.0 33.0 40.0 2.0 41.0 48-49 30.058175855067937 35.0 31.0 39.5 2.0 41.0 50-51 29.000555295260902 35.0 27.5 39.0 2.0 41.0 52-53 28.19521231367241 35.0 24.0 38.0 2.0 41.0 54-55 27.526636819546393 35.0 14.0 38.0 2.0 41.0 56-57 26.551035105072273 35.0 2.0 38.0 2.0 40.5 58-59 25.569021465632428 34.5 2.0 38.0 2.0 40.0 60-61 24.371223558401443 33.5 2.0 37.0 2.0 40.0 62-63 23.36417824977875 33.0 2.0 36.0 2.0 40.0 64-65 22.244694327311848 32.5 2.0 36.0 2.0 39.5 66-67 20.855319555069673 30.5 2.0 35.0 2.0 39.0 68-69 19.59990108803165 27.5 2.0 35.0 2.0 38.0 70-71 18.737319312127994 24.5 2.0 35.0 2.0 37.0 72-73 18.264259808770195 21.0 2.0 35.0 2.0 36.5 74-75 17.895135960574038 19.0 2.0 35.0 2.0 35.5 76-77 17.070609263019072 12.5 2.0 33.0 2.0 35.0 78-79 17.40631995418814 6.5 2.0 34.0 2.0 35.0 80-81 17.33060023947108 2.0 2.0 34.0 2.0 35.0 82-83 17.089350478074515 2.0 2.0 34.0 2.0 35.0 84-85 16.813472851267633 2.0 2.0 34.0 2.0 35.0 86-87 16.568153816787273 2.0 2.0 34.0 2.0 35.0 88-89 16.203628507470455 2.0 2.0 34.0 2.0 35.0 90-91 15.955064466309196 2.0 2.0 34.0 2.0 35.0 92-93 15.632403213771322 2.0 2.0 33.5 2.0 35.0 94-95 15.286749266836726 2.0 2.0 33.0 2.0 35.0 96-97 14.865245457858297 2.0 2.0 33.0 2.0 35.0 98-99 14.401478473632151 2.0 2.0 33.0 2.0 35.0 100-101 12.818054037170075 2.0 2.0 29.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.44353492110251835 1101 2 0.5006771902202871 1101 3 0.6849891082831903 1101 4 0.586126735249124 1101 5 0.5176247788224941 1101 6 0.4384149303014624 1101 7 0.35505855338770687 1101 8 0.3847822029620147 1101 9 0.7260970212172495 1101 10-11 0.6444416516594984 1101 12-13 0.6280071987119555 1101 14-15 1.2096915924133569 1101 16-17 1.0704438736628745 1101 18-19 0.8155942755200769 1101 20-21 0.35280607327453595 1101 22-23 0.0817565923040604 1101 24-25 0.12604854774607333 1101 26-27 -0.00917427315231123 1101 28-29 -0.05912570081613211 1101 30-31 0.2415509133584166 1101 32-33 1.125869345052429 1101 34-35 1.3328286789308805 1101 36-37 0.8354300234200522 1101 38-39 2.06539327324068 1101 40-41 2.010974541799804 1101 42-43 1.753483381673007 1101 44-45 1.599734680201614 1101 46-47 2.596029317906229 1101 48-49 3.519750164285913 1101 50-51 3.751492562366021 1101 52-53 2.9068004296887437 1101 54-55 2.274496058460816 1101 56-57 2.218707422080108 1101 58-59 2.6780337882835745 1101 60-61 3.003676927471261 1101 62-63 4.712957435945814 1101 64-65 4.301322585737903 1101 66-67 3.1187002084983177 1101 68-69 3.7995188356051095 1101 70-71 3.4271657698758773 1101 72-73 4.060565143407889 1101 74-75 3.7896033102721383 1101 76-77 3.475662228476235 1101 78-79 4.035901915120537 1101 80-81 4.05246287592345 1101 82-83 3.722183293693977 1101 84-85 4.153352762164673 1101 86-87 3.5991672336800455 1101 88-89 4.385379764174566 1101 90-91 5.028787081133112 1101 92-93 4.769020418347473 1101 94-95 3.905089817223528 1101 96-97 3.9540514479039945 1101 98-99 4.079996089278675 1101 100-101 4.151600982596742 1102 1 0.23753078031576536 1102 2 -0.20843254062857142 1102 3 -0.4806423616961055 1102 4 -0.04223765398482726 1102 5 0.22569931298399126 1102 6 -0.1878790655577447 1102 7 0.2867355720212501 1102 8 -0.23737100614771833 1102 9 0.3534262137638322 1102 10-11 0.14651204503423543 1102 12-13 0.06123701237654444 1102 14-15 0.5202505986245356 1102 16-17 0.26557844923222973 1102 18-19 -0.08347404746129428 1102 20-21 0.005497584661696919 1102 22-23 -0.07041732073942342 1102 24-25 -0.12343385391024242 1102 26-27 -0.13080988391836001 1102 28-29 -0.22993315423228466 1102 30-31 0.2927931493832574 1102 32-33 -0.5387269282394911 1102 34-35 -0.5341485467419957 1102 36-37 -0.7468681132259078 1102 38-39 -0.15665641620031323 1102 40-41 -0.7478246300428495 1102 42-43 -0.6926863905837166 1102 44-45 0.5070845766819474 1102 46-47 1.1772922578648206 1102 48-49 0.5150917791927405 1102 50-51 1.029960471268712 1102 52-53 0.5713967029806675 1102 54-55 -0.18823686079384316 1102 56-57 0.45576746348797315 1102 58-59 0.508261531554794 1102 60-61 0.5476727866845081 1102 62-63 -0.5282433922115395 1102 64-65 0.3623991902927699 1102 66-67 -0.7125627314602738 1102 68-69 -0.6414749532147646 1102 70-71 0.22323202246386842 1102 72-73 0.6475216651470213 1102 74-75 0.8951933723839396 1102 76-77 0.853509019366502 1102 78-79 0.6948046066319229 1102 80-81 0.7600198117412518 1102 82-83 0.16214188582648248 1102 84-85 0.05345628183340878 1102 86-87 -0.6161536772930383 1102 88-89 -0.40085211988340674 1102 90-91 -1.1896394199020843 1102 92-93 -1.5301514243026304 1102 94-95 -1.7036058349503858 1102 96-97 -1.3332156328413465 1102 98-99 -0.5209355877399577 1102 100-101 0.24735667617852108 1103 1 0.5244873020549008 1103 2 0.44591528545838344 1103 3 0.28022720352128516 1103 4 0.6099362590586495 1103 5 0.5104819216796415 1103 6 0.261033977920512 1103 7 0.01934426767342501 1103 8 0.2550202982001082 1103 9 0.14038273550296765 1103 10-11 0.8057511754690196 1103 12-13 0.8101500558548125 1103 14-15 0.9686201638419263 1103 16-17 0.6644914927104892 1103 18-19 0.11380856123436445 1103 20-21 0.12125845422691839 1103 22-23 -0.5682434076959382 1103 24-25 -0.6429990713015457 1103 26-27 -0.13298379696183815 1103 28-29 -0.18591141510184173 1103 30-31 -0.5191633723558766 1103 32-33 0.13301220219529597 1103 34-35 -0.2124094163072172 1103 36-37 0.9265014519914843 1103 38-39 0.8368218446692524 1103 40-41 0.3347840656093197 1103 42-43 -0.23996899927936965 1103 44-45 0.609258489725427 1103 46-47 0.9555531274300364 1103 48-49 2.2036787357144796 1103 50-51 1.4913735147469716 1103 52-53 2.049657572545886 1103 54-55 0.9530674870322429 1103 56-57 1.4198978982705839 1103 58-59 1.7506528359026206 1103 60-61 2.0096293084236407 1103 62-63 1.8266479121362877 1103 64-65 -0.9212964618811448 1103 66-67 -0.14082360102549174 1103 68-69 0.06559026417653868 1103 70-71 -0.5680723253622162 1103 72-73 -1.0144348565921106 1103 74-75 -1.2961109754421436 1103 76-77 -0.9040996762856714 1103 78-79 -0.39921713249851365 1103 80-81 -0.19813236217179053 1103 82-83 -0.49329289678221144 1103 84-85 -0.6079567616448536 1103 86-87 -0.45202324251042825 1103 88-89 -0.5687869024921017 1103 90-91 -0.40811768077165134 1103 92-93 0.553544227871285 1103 94-95 -0.31991018277647143 1103 96-97 0.8504800193325668 1103 98-99 0.40678180356439064 1103 100-101 -0.5222085412127839 1104 1 -0.06440158683398778 1104 2 -0.23741804787495369 1104 3 -0.3494024261083446 1104 4 0.01734366646605423 1104 5 -0.6913699301722147 1104 6 -0.8658178739313414 1104 7 -0.8565816582525017 1104 8 -0.05701673883692848 1104 9 0.18112347624370528 1104 10-11 0.14047339769124534 1104 12-13 -0.10744253673778559 1104 14-15 0.18852757124933106 1104 16-17 -0.42161961840061934 1104 18-19 -0.09313588321008126 1104 20-21 -0.2014267309582749 1104 22-23 -0.9089841484366801 1104 24-25 -0.4096657379682185 1104 26-27 -0.3200208339988748 1104 28-29 -0.551189192879626 1104 30-31 -0.7756448538373562 1104 32-33 -1.2669877978047097 1104 34-35 -1.290187194084993 1104 36-37 -0.6063689183788838 1104 38-39 -1.4187337108863076 1104 40-41 -1.6226233417980822 1104 42-43 -0.6737652955756701 1104 44-45 -1.877778547311614 1104 46-47 -2.1592616873847774 1104 48-49 -1.86252496798922 1104 50-51 -2.4257561148826596 1104 52-53 -1.3577498348615187 1104 54-55 -0.9358214018566429 1104 56-57 -2.777324323951639 1104 58-59 -2.1910138307640423 1104 60-61 -2.5690743952800617 1104 62-63 -1.6812224582340853 1104 64-65 -1.8481483137329953 1104 66-67 -2.4408236010254925 1104 68-69 -2.8645023284160516 1104 70-71 -2.5291834364733283 1104 72-73 -2.33758300474026 1104 74-75 -2.3109257902569613 1104 76-77 -2.447618194804189 1104 78-79 -2.0209763917577703 1104 80-81 -2.278224954764383 1104 82-83 -2.5331077115970277 1104 84-85 -2.6945308357189273 1104 86-87 -1.85850472399191 1104 88-89 -2.0035091247143235 1104 90-91 -2.0757102733642423 1104 92-93 -1.764048364721308 1104 94-95 -1.1412064790727694 1104 96-97 -1.7800755362229879 1104 98-99 -1.5853478260652398 1104 100-101 -1.6870233560275985 1105 1 -0.1573308797632862 1105 2 0.5231880127311115 1105 3 0.3256817489758319 1105 4 -0.16279101366862392 1105 5 -0.09406353286581748 1105 6 -0.39578420389766933 1105 7 -0.3852011868720311 1105 8 -0.7836160654362558 1105 9 -1.0505263554061273 1105 10-11 -0.3272033699855328 1105 12-13 -0.06939539869064504 1105 14-15 0.5129383456601104 1105 16-17 0.334946038165036 1105 18-19 0.1285812885070925 1105 20-21 -0.770787000318542 1105 22-23 -0.08187977133230362 1105 24-25 -0.2793627076651788 1105 26-27 -0.18298379696184242 1105 28-29 -0.28704777873821286 1105 30-31 -0.330527008719514 1105 32-33 0.019375838558922 1105 34-35 0.6921360382382389 1105 36-37 -1.3450894570994265 1105 38-39 -1.694996337148929 1105 40-41 -1.142488661663407 1105 42-43 -1.4547417265521005 1105 44-45 -0.8680142375473032 1105 46-47 -1.7808105089335982 1105 48-49 -1.6770030824673334 1105 50-51 -1.855217394343942 1105 52-53 -1.345796972908662 1105 54-55 -0.6378416038768506 1105 56-57 -0.6732839199112348 1105 58-59 0.2029255631753486 1105 60-61 -0.9926434188490845 1105 62-63 -0.8835793605909821 1105 64-65 -1.4826600982447786 1105 66-67 -0.8590054192073104 1105 68-69 -1.6719097358234585 1105 70-71 -1.4180723253622176 1105 72-73 -0.9394348565921113 1105 74-75 -0.3688382481694177 1105 76-77 -0.4154633126493046 1105 78-79 -0.2298989506803295 1105 80-81 -0.4629050894445186 1105 82-83 -1.110786003941655E-4 1105 84-85 -0.21591130709939677 1105 86-87 0.23661312112593436 1105 88-89 0.5278040065988101 1105 90-91 0.6714277737738055 1105 92-93 0.8103624096894677 1105 94-95 0.7732716354053455 1105 96-97 1.4527527466052934 1105 98-99 2.007918167200751 1105 100-101 2.0823369133326697 1106 1 -0.00243577486818225 1106 2 0.07668451622761552 1106 3 -0.06592664263256154 1106 4 0.32147472059710935 1106 5 0.17971269091040654 1106 6 -0.2255044836179465 1106 7 0.05011349844265567 1106 8 -0.1545950864152772 1106 9 -0.3480788029585753 1106 10-11 0.4567127139305569 1106 12-13 -0.09177302106827057 1106 14-15 0.2330432407650065 1106 16-17 0.16064533886434162 1106 18-19 0.1590008689266682 1106 20-21 0.012604608073068846 1106 22-23 -0.3893972538497863 1106 24-25 -0.6372298405323136 1106 26-27 -0.10029148926953013 1106 28-29 0.293896277205846 1106 30-31 0.4942981661056578 1106 32-33 0.3580122021952903 1106 34-35 0.5741290452312526 1106 36-37 0.43707837506840264 1106 38-39 0.4637449215923297 1106 40-41 0.6309379117631693 1106 42-43 0.8052233084129341 1106 44-45 1.2554123358792708 1106 46-47 -0.12329302641611406 1106 48-49 -0.8126674181316709 1106 50-51 0.3481042839777402 1106 52-53 0.15542680331511605 1106 54-55 -0.2777017437369871 1106 56-57 -0.03587133249865104 1106 58-59 0.10065283590262197 1106 60-61 -0.13460146080712931 1106 62-63 0.37184021982859505 1106 64-65 -0.8616810772657573 1106 66-67 -2.171592831794726 1106 68-69 -1.9065251204388467 1106 70-71 -1.2738415561314476 1106 72-73 -0.40097331813057124 1106 74-75 -0.1691878985190698 1106 76-77 -0.55409967628567 1106 78-79 -0.5982555940369707 1106 80-81 0.12205994552051536 1106 82-83 0.5317071032177871 1106 84-85 0.38915862297053394 1106 86-87 0.8537459882588045 1106 88-89 0.39179002058482126 1106 90-91 0.824574626920656 1106 92-93 -0.09453269520563978 1106 94-95 0.3762436633773749 1106 96-97 -0.032212288359739105 1106 98-99 0.928897188179775 1106 100-101 0.8874068434025997 1107 1 0.600349371020414 1107 2 0.3545359751135564 1107 3 0.4181582380040467 1107 4 0.4720052245758879 1107 5 0.5742750251279176 1107 6 0.451551219299823 1107 7 0.465895991811351 1107 8 0.4455375395794192 1107 9 0.76796894239952 1107 10-11 0.651009796158668 1107 12-13 0.8127362627513648 1107 14-15 1.0656029224626167 1107 16-17 0.6550087340898045 1107 18-19 0.8426878715791872 1107 20-21 1.0096205231924316 1107 22-23 0.771411764717854 1107 24-25 0.7397595493881113 1107 26-27 0.9325334444174729 1107 28-29 0.7783127228291846 1107 30-31 0.886871110402744 1107 32-33 0.8278397884021871 1107 34-35 1.4013836871410632 1107 36-37 1.0484842106121661 1107 38-39 1.4040632239795912 1107 40-41 1.9399564794024258 1107 42-43 2.1483930696861435 1107 44-45 1.6420171104150825 1107 46-47 1.577966920533484 1107 48-49 0.9704890805420696 1107 50-51 1.298701100953867 1107 52-53 1.1531058484079573 1107 54-55 1.7289295559977589 1107 56-57 0.7724841051671341 1107 58-59 1.225652835902622 1107 60-61 0.8501465498029503 1107 62-63 1.2925961879983596 1107 64-65 0.39163457260161394 1107 66-67 0.6971074334572691 1107 68-69 0.10740060900412729 1107 70-71 0.5991690539481276 1107 72-73 0.37090997099409506 1107 74-75 0.46940626593716317 1107 76-77 0.2864175650936396 1107 78-79 0.5400070054325248 1107 80-81 0.37471246541441516 1107 82-83 0.05756917218330315 1107 84-85 0.15540530732066316 1107 86-87 0.09711468852405503 1107 88-89 0.2187130975078979 1107 90-91 0.4479168019869686 1107 92-93 0.9319925037333547 1107 94-95 0.4137105068787008 1107 96-97 0.9651351917463611 1107 98-99 1.5011783552885287 1107 100-101 0.7571018036148036 1108 1 0.0867823840221078 1108 2 -0.05982241945964972 1108 3 0.042522285488495015 1108 4 0.09354281643570062 1108 5 0.07851470856488874 1108 6 0.10078807628116948 1108 7 0.13491803816522463 1108 8 0.11116783918371453 1108 9 -0.03502710056260838 1108 10-11 0.03300527382967289 1108 12-13 -0.06403027201404399 1108 14-15 -0.14920770501053227 1108 16-17 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72-73 -1.1407335578908153 2316 74-75 -0.6909161702473412 2316 76-77 -1.1336451308311233 2316 78-79 -1.0247041454855221 2316 80-81 -1.130437556976986 2316 82-83 -0.9845266630159806 2316 84-85 -1.0977294889175795 2316 86-87 -0.776373891861077 2316 88-89 -0.7877804089856077 2316 90-91 -0.6584423560963248 2316 92-93 -0.3922349929079374 2316 94-95 -0.6890660269323146 2316 96-97 -0.40309140923885955 2316 98-99 -0.2609130016304153 2316 100-101 -0.554026723030967 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 2.0 5 9.0 6 23.0 7 44.0 8 84.0 9 151.0 10 261.0 11 461.0 12 709.0 13 968.0 14 1229.0 15 1569.0 16 2092.0 17 2508.0 18 2599.0 19 2034.0 20 1961.0 21 2160.0 22 2060.0 23 2154.0 24 2278.0 25 2249.0 26 1469.0 27 820.0 28 747.0 29 848.0 30 960.0 31 1089.0 32 1296.0 33 1615.0 34 2501.0 35 5408.0 36 4432.0 37 5947.0 38 2757.0 39 133.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.71594912107172 23.742342998941467 14.770853939993406 14.770853939993406 2 15.118276957253684 26.135476145021606 39.193668755098145 19.552578142626565 3 21.444189920866304 35.672636401499375 33.814035818408996 9.069137859225322 4 10.174050358338974 27.54958613150086 29.659708122928492 32.61665538723168 5 6.412036233644535 44.69510290493874 33.21938014090862 15.673480720508104 6 18.23797872525032 40.902701858503825 13.615145678241102 27.244173738004758 7 12.66940843701737 45.75459420063512 18.791538688462005 22.784458673885506 8 23.704166449754453 32.757214500147505 19.497804848421747 24.040814201676298 9 21.224426050288926 20.660454300935324 19.582834435247367 38.53228521352838 10-11 21.239176080656634 31.377652836344073 21.768441876203863 25.614729206795428 12-13 28.635014836795254 29.894840959966686 24.028667117844066 17.441477085394 14-15 24.229094001075886 30.37551842018498 27.48190952157843 17.913478057160706 16-17 14.830721710309403 37.07637045135093 27.74480712166172 20.348100716677948 18-19 14.182588022975343 31.440123553195548 35.34020511218699 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10.931588629342958 32.87025788761237 39.833744056089685 16.364409426954982 46-47 14.024173326044892 30.769364257143106 37.8651812131782 17.341281203633805 48-49 13.171891404128308 29.34327089099633 38.15605667531431 19.32878102956105 50-51 10.418727332673921 29.751678900515383 41.165599458587124 18.663994308223575 52-53 11.65165634164541 30.234091658424 41.06408454370347 17.050167456227115 54-55 10.888993006750308 29.00289794714283 38.36135839103198 21.74675065507488 56-57 14.897530671386678 30.376386069030143 33.81748138893227 20.908601870650912 58-59 12.641643673972268 32.9238030784181 36.53582522081663 17.898728026792995 60-61 13.552674961389627 36.54189876273274 31.628403352595136 18.27702292328249 62-63 10.582712964409044 38.634667777257185 31.107814045499506 19.67480521283426 64-65 10.797889878008572 38.629461884186234 30.708695576726186 19.863952661079008 66-67 11.527582556787618 36.13236850781752 30.47790098391379 21.862147951481077 68-69 11.87724504138685 34.10814375206066 31.240564318808893 22.774046887743594 70-71 13.296718552067608 32.02144827945234 31.123431724712376 23.55840144376768 72-73 12.96961493744252 28.604647127214673 33.258715532649624 25.167022402693185 74-75 12.894129487913652 28.81027990351745 32.12556614087146 26.170024467697434 76-77 12.757040970378469 27.13745293005015 33.95717285300293 26.148333246568452 78-79 13.182188904506567 26.548319364186927 34.48383570201468 25.785656029291825 80-81 13.315806826661113 26.03467124785257 33.682995817932564 26.96652610755375 82-83 12.759976052268525 25.538173204570892 33.87215729147686 27.829693451683717 84-85 13.182007496876302 25.830383173677635 32.62095654588366 28.366652783562408 86-87 13.32373126946804 25.624495674732977 32.080726748978336 28.971046306820647 88-89 13.47805716070592 24.76356568969407 30.55945997535877 31.19891717424124 90-91 13.671505548951382 23.698664607321664 31.481079767803067 31.148750075923886 92-93 13.846060406236713 24.147744527257426 31.362307271828065 30.643887794677795 94-95 13.53271903795096 23.797872525031668 30.931681329932147 31.737727107085224 96-97 12.6217972634118 24.17550953120526 31.0933337960834 32.10935940929954 98-99 13.776647896367123 24.531248644286915 29.511596227430086 32.180507231915875 100-101 13.354215111234513 23.83125672439524 29.976052476312777 32.83847568805747 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1943.0 1 1102.5 2 333.0 3 408.0 4 273.5 5 180.0 6 383.5 7 657.5 8 826.0 9 856.0 10 615.5 11 330.0 12 257.0 13 262.5 14 261.5 15 273.0 16 281.5 17 289.0 18 320.5 19 334.0 20 373.5 21 440.5 22 498.0 23 569.5 24 626.5 25 673.5 26 732.5 27 801.0 28 906.5 29 1008.5 30 1064.5 31 1147.5 32 1287.5 33 1344.0 34 1337.0 35 1336.0 36 1287.5 37 1203.5 38 1113.0 39 1012.5 40 946.0 41 958.0 42 1011.0 43 1073.0 44 1156.5 45 1224.0 46 1334.5 47 2029.0 48 3464.5 49 3580.0 50 3002.0 51 3168.0 52 2790.5 53 1721.0 54 738.0 55 322.5 56 220.0 57 189.0 58 149.5 59 117.0 60 88.5 61 74.5 62 62.0 63 47.5 64 33.0 65 25.5 66 28.5 67 26.0 68 17.5 69 14.5 70 10.5 71 8.0 72 7.0 73 4.0 74 3.0 75 2.0 76 0.0 77 1.0 78 1.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.013882381522550193 3 0.005205893070956323 4 0.0 5 0.0017352976903187742 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0026029465354781615 26-27 0.019955923438665903 28-29 0.0 30-31 0.0 32-33 0.004338244225796936 34-35 0.016485328058028353 36-37 0.008676488451593871 38-39 0.019088274593506517 40-41 0.02082357228382529 42-43 0.016485328058028353 44-45 0.008676488451593871 46-47 0.0026029465354781615 48-49 8.676488451593871E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0026029465354781615 84-85 0.005205893070956323 86-87 8.676488451593871E-4 88-89 0.0 90-91 0.00607354191611571 92-93 8.676488451593871E-4 94-95 0.0 96-97 8.676488451593871E-4 98-99 8.676488451593871E-4 100-101 0.0017352976903187742 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 57627.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.57740295347667 #Duplication Level Percentage of deduplicated Percentage of total 1 85.91606315505376 33.14418588508859 2 7.111690882101569 5.487011296787965 3 2.2401151545139673 2.5925347493362487 4 1.2010255948900186 1.853297933260451 5 0.6747334802752912 1.3014732677390806 6 0.4273312041743511 0.9891196834817012 7 0.3283702937339751 0.8867371197528937 8 0.2159147136880932 0.6663543130824093 9 0.179928928073411 0.6247071685147587 >10 1.371958076559759 10.295521196661287 >50 0.13044847285322297 3.6180956843146443 >100 0.15293958886239933 12.042965970812293 >500 0.022491116009176373 5.743835354955142 >1k 0.02698933921101165 20.75416037621254 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT 2674 4.640186023912403 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2220 3.8523608725076786 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2100 3.6441251496694256 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2012 3.4914189529213733 No Hit ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT 1542 2.6758290384715497 RNA PCR Primer, Index 31 (95% over 22bp) TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1412 2.4502403387301093 No Hit ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT 850 1.4750030367709581 RNA PCR Primer, Index 31 (95% over 24bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 685 1.1886789178683603 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 678 1.176531834036129 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 565 0.9804431950301075 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 532 0.9231783712495879 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 472 0.8190605098304614 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 413 0.7166779461016537 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 378 0.6559425269404966 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 342 0.5934718100890208 No Hit CATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCT 318 0.5518246655213702 RNA PCR Primer, Index 31 (96% over 27bp) TTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC 291 0.5049716278827633 No Hit CACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTG 259 0.44944210179256255 RNA PCR Primer, Index 38 (95% over 24bp) ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 252 0.4372950179603311 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 242 0.41994204105714333 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 221 0.3835007895604491 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 211 0.3661478126572614 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 203 0.3522654311347112 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 202 0.3505301334443924 No Hit TATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCT 198 0.3435889426831173 RNA PCR Primer, Index 31 (95% over 21bp) ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 189 0.3279712634702483 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 187 0.32450066808961076 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 178 0.3088829888767418 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 177 0.30714769118642304 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 175 0.30367709580578545 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 166 0.2880594165929165 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 164 0.28458882121227896 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 156 0.27070643968972874 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 150 0.26029465354781617 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 146 0.25335346278654103 No Hit CTTTACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC 141 0.24467697433494714 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 132 0.2290592951220782 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 132 0.2290592951220782 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 132 0.2290592951220782 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 127 0.22038280667048432 No Hit ACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGC 123 0.21344161590920924 RNA PCR Primer, Index 38 (96% over 25bp) AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 118 0.20476512745761535 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 117 0.2030298297672966 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 116 0.20129453207697778 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 112 0.19435334131570273 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 100 0.17352976903187742 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 99 0.17179447134155865 No Hit CTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC 94 0.16311798288996476 No Hit GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACGCTACGC 92 0.15964738750932722 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 91 0.15791208981900845 No Hit GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 88 0.15270619674805214 No Hit GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 88 0.15270619674805214 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 86 0.1492356013674146 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 84 0.14576500598677702 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 76 0.13188262446422683 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 75 0.13014732677390808 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 73 0.12667673139327051 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 72 0.12494143370295174 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 71 0.12320613601263296 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 71 0.12320613601263296 No Hit GGTATCAACGCAGAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 67 0.11626494525135786 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 64 0.11105905218040155 No Hit GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA 64 0.11105905218040155 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 63 0.10932375449008278 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 63 0.10932375449008278 No Hit GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 63 0.10932375449008278 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 59 0.10238256372880768 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.005205893070956323 0.0 22-23 0.0 0.0 0.0 0.03557360265153487 0.0 24-25 0.0 0.0 0.0 0.09804431950301074 0.0 26-27 0.0 0.0 0.0 0.21864750898016555 0.0 28-29 0.0 0.0 0.0 0.5379422839988199 0.0 30-31 0.0 0.0 0.0 1.1392229336942752 0.0 32-33 0.0 0.0 0.0 1.8290037655959879 0.0 34-35 0.0 0.0 0.0 2.8233293421486456 0.0 36-37 0.0 0.0 0.0 4.095302549152307 0.0 38-39 0.0 0.0 0.0 5.3707463515366065 0.0 40-41 0.0 0.0 0.0 6.475263331424506 0.0 42-43 0.0 0.0 0.0 7.3377062835129365 0.0 44-45 0.0 0.0 0.0 8.156766793343397 0.0 46-47 0.0 0.0 0.0 8.870841792909573 0.0 48-49 0.0 0.0 0.0 9.651725753553023 0.0 50-51 0.0 0.0 0.0 10.26081524285491 0.0 52-53 0.0 0.0 0.0 10.70071320735072 0.0 54-55 0.0 0.0 0.0 11.247331979801135 0.0 56-57 0.0 0.0 0.0 11.898068613670675 0.0 58-59 0.0 0.0 0.0 12.3162753570375 0.0 60-61 0.0 0.0 0.0 12.724070314262413 0.0 62-63 0.0 0.0 0.0 13.085879882693876 0.0 64-65 0.0 0.0 0.0 13.412983497318965 0.0 66-67 0.0 0.0 0.0 13.791278393808458 0.0 68-69 0.0 0.0 0.0 14.217293976781717 0.0 70-71 0.0 0.0 0.0 14.677147864716193 0.0 72-73 0.0 0.0 0.0 15.06325160081212 0.0 74-75 0.0 0.0 0.0 15.412046436566193 0.0 76-77 0.0 0.0 0.0 15.72613531851389 0.0 78-79 0.0 0.0 0.0 16.131327329203323 0.0 80-81 0.0 0.0 0.0 16.473180974196126 0.0 82-83 0.0 0.0 0.0 16.771652176930953 0.0 84-85 0.0 0.0 0.0 17.136064691897893 0.0 86-87 0.0 0.0 0.0 17.47184479497458 0.0 88-89 0.0 0.0 0.0 17.777257188470685 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAAGA 30 1.2931407E-4 63.31018 9 ATGTGTA 30 1.2931407E-4 63.31018 4 GAGATGT 30 1.2931407E-4 63.31018 1 ACCCATG 25 0.0050043943 56.979164 3 CTTTACA 35 2.770711E-4 54.265873 1 GTGTATA 40 5.3550507E-4 47.482635 6 GATGTGT 40 5.3550507E-4 47.482635 3 TGTATAA 40 5.3550507E-4 47.482635 7 CGCAGAA 60 8.2106795E-5 39.568863 9 GTATAAG 50 0.0016059844 37.98611 8 AGATGTG 50 0.0016059844 37.98611 2 GAGTAAT 45 2.400294E-5 31.655092 12-13 GTAATGG 45 2.400294E-5 31.655092 14-15 TAAGAGA 30 0.0039433907 31.65509 10-11 CATGGGG 120 1.4687976E-7 31.65509 4 TGTGTAT 60 0.003927164 31.65509 5 GCCCTGT 35 0.008340299 27.156511 38-39 ATAAGAG 35 0.008375734 27.132936 10-11 TAATGGG 35 0.008375734 27.132936 14-15 AGACAGA 35 0.008375734 27.132936 14-15 >>END_MODULE