##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937989_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 58526 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.13472644636572 33.0 31.0 34.0 30.0 34.0 2 32.3347913747736 33.0 31.0 34.0 30.0 34.0 3 32.255629976420735 34.0 31.0 34.0 30.0 34.0 4 35.84591463623005 37.0 35.0 37.0 35.0 37.0 5 35.85965212042511 37.0 35.0 37.0 35.0 37.0 6 35.99870143184226 37.0 35.0 37.0 35.0 37.0 7 35.93958240781875 37.0 35.0 37.0 35.0 37.0 8 36.00428869220517 37.0 35.0 37.0 35.0 37.0 9 37.53301096948365 39.0 37.0 39.0 35.0 39.0 10-11 37.58976352390391 39.0 37.0 39.0 35.0 39.0 12-13 37.52495472097871 39.0 37.0 39.0 35.0 39.0 14-15 38.42092403376277 40.0 38.0 41.0 33.5 41.0 16-17 38.30639373953457 40.0 37.5 41.0 33.5 41.0 18-19 38.47619006937088 40.0 37.5 41.0 34.0 41.0 20-21 38.587405597512216 40.0 37.5 41.0 35.0 41.0 22-23 38.38591395277312 40.0 37.0 41.0 34.5 41.0 24-25 38.1592112907084 40.0 36.5 41.0 34.0 41.0 26-27 37.7789529439907 40.0 35.5 41.0 33.5 41.0 28-29 37.49453234459898 39.5 35.0 41.0 33.0 41.0 30-31 36.8293664354304 38.5 35.0 40.5 33.0 41.0 32-33 35.99398557905888 36.5 35.0 40.0 32.0 41.0 34-35 35.36493182517172 35.5 35.0 40.0 31.0 41.0 36-37 34.814928407887095 35.0 35.0 40.0 30.0 41.0 38-39 34.13742610121997 35.0 35.0 40.0 25.5 41.0 40-41 33.764660151043984 35.0 35.0 40.0 22.5 41.0 42-43 33.1037060451765 35.0 34.5 40.0 12.5 41.0 44-45 32.294980008884934 35.0 33.0 40.0 2.0 41.0 46-47 31.17517855312169 35.0 33.0 40.0 2.0 41.0 48-49 30.103808563715273 35.0 31.5 39.5 2.0 41.0 50-51 29.036488056590233 35.0 27.5 39.0 2.0 41.0 52-53 28.212170659194207 35.0 23.0 38.5 2.0 41.0 54-55 27.571523767214572 35.0 11.0 38.0 2.0 41.0 56-57 26.64033079315176 35.0 2.0 38.0 2.0 41.0 58-59 25.729778218227796 35.0 2.0 38.0 2.0 41.0 60-61 24.55113966442265 34.0 2.0 37.5 2.0 40.0 62-63 23.63162526056795 33.0 2.0 37.0 2.0 40.0 64-65 22.558273246078667 33.0 2.0 36.0 2.0 39.5 66-67 21.240371800567267 31.5 2.0 35.5 2.0 39.0 68-69 20.00310972900933 29.5 2.0 35.0 2.0 38.5 70-71 19.105175477565528 26.0 2.0 35.0 2.0 37.5 72-73 18.611480367699826 24.0 2.0 35.0 2.0 37.0 74-75 18.276466869425555 21.5 2.0 35.0 2.0 36.0 76-77 17.400804770529337 18.0 2.0 33.5 2.0 35.0 78-79 17.74906024672795 14.5 2.0 35.0 2.0 35.0 80-81 17.582074633496227 4.5 2.0 35.0 2.0 35.0 82-83 17.360292861292418 2.0 2.0 35.0 2.0 35.0 84-85 17.11359908416772 2.0 2.0 35.0 2.0 35.0 86-87 16.887793459317226 2.0 2.0 34.0 2.0 35.0 88-89 16.589302190479444 2.0 2.0 34.0 2.0 35.0 90-91 16.377524519017186 2.0 2.0 34.0 2.0 35.0 92-93 16.180159245463557 2.0 2.0 34.0 2.0 35.0 94-95 15.975771452004238 2.0 2.0 34.0 2.0 35.0 96-97 15.670462700338312 2.0 2.0 34.0 2.0 35.0 98-99 15.322497693332878 2.0 2.0 34.0 2.0 35.0 100-101 14.255125926938454 2.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1291968287312386 1101 2 0.018054752864948398 1101 3 0.19307219070005743 1101 4 -0.35600213354227606 1101 5 0.24921417707857785 1101 6 0.10447624604613992 1101 7 0.029759774872601952 1101 8 -0.17108158823135255 1101 9 -0.2664488930605202 1101 10-11 -0.041116201471183444 1101 12-13 0.14997966502570392 1101 14-15 0.3795952063010404 1101 16-17 0.4874315420790154 1101 18-19 0.38951206912410896 1101 20-21 -0.22652772384557807 1101 22-23 0.2701550447669021 1101 24-25 0.5642293009299024 1101 26-27 0.14039199058231588 1101 28-29 0.28342084243237764 1101 30-31 0.47609996093466833 1101 32-33 1.0535153772777264 1101 34-35 1.1325262980706867 1101 36-37 1.2929832625234141 1101 38-39 1.7583652776237741 1101 40-41 2.269706027370752 1101 42-43 2.3194099999398716 1101 44-45 3.05794931259954 1101 46-47 2.2400371116124127 1101 48-49 3.290116428728883 1101 50-51 2.737546247165117 1101 52-53 1.0478323127674543 1101 54-55 -0.8136555106455532 1101 56-57 -0.0033128248514593395 1101 58-59 0.7371773563279618 1101 60-61 1.1079463426492175 1101 62-63 1.1688801941313116 1101 64-65 0.5968897486742826 1101 66-67 0.8780139221272627 1101 68-69 0.9329173512220557 1101 70-71 1.5123043081649428 1101 72-73 2.651179821041733 1101 74-75 2.0385296537744573 1101 76-77 2.381626074047272 1101 78-79 2.0982692937873253 1101 80-81 1.5452567711464162 1101 82-83 1.3860599060698 1101 84-85 2.0336450619424404 1101 86-87 1.756248730762504 1101 88-89 1.7212574950061388 1101 90-91 1.9380942392066345 1101 92-93 1.8981426328139506 1101 94-95 2.4109401988560357 1101 96-97 2.891797314316916 1101 98-99 1.4765687661452933 1101 100-101 2.041620815051294 1102 1 -0.32348609809802653 1102 2 -0.03218914957407293 1102 3 -0.09473268734871709 1102 4 -0.008197255493499256 1102 5 -0.17712728633605934 1102 6 -0.13747497346605542 1102 7 -0.6458499812249627 1102 8 -0.20474012481671622 1102 9 -0.24352206379222707 1102 10-11 -0.33989668927605976 1102 12-13 -0.2917276520474701 1102 14-15 -0.0662584522355445 1102 16-17 -0.12964162865268491 1102 18-19 0.24926816668508422 1102 20-21 0.008838129812957618 1102 22-23 0.32503309354738974 1102 24-25 0.18032686190550606 1102 26-27 0.3313676003384103 1102 28-29 0.20732328145676604 1102 30-31 -0.03121711223605672 1102 32-33 1.0266861089850465 1102 34-35 1.0925262980706805 1102 36-37 1.1232271649624366 1102 38-39 0.7603164971359675 1102 40-41 1.1287304176146549 1102 42-43 1.3016051218910931 1102 44-45 1.6467298004044153 1102 46-47 0.5256468677099733 1102 48-49 -0.15207869322233947 1102 50-51 0.017058442287069653 1102 52-53 0.9795396298406303 1102 54-55 1.049027416183712 1102 56-57 0.49668717514854066 1102 58-59 0.6301041855962559 1102 60-61 1.3489219524053162 1102 62-63 0.6954655599849744 1102 64-65 0.3622556023328194 1102 66-67 1.2421602635906765 1102 68-69 1.3465758878074219 1102 70-71 1.728645771579579 1102 72-73 1.3889846990905106 1102 74-75 1.5841394098720194 1102 76-77 0.5160163179497097 1102 78-79 0.29070831817756826 1102 80-81 0.31501286870739165 1102 82-83 -0.348330337832639 1102 84-85 0.22901091560097697 1102 86-87 0.19502921856738098 1102 88-89 0.6615013974451642 1102 90-91 -0.11385698030555957 1102 92-93 0.7957036084237039 1102 94-95 0.4836231256853054 1102 96-97 0.8949680460242302 1102 98-99 1.2002273027306547 1102 100-101 0.975523254075684 1103 1 -0.14348609809802326 1103 2 -0.2521891495740789 1103 3 -0.1747326873487225 1103 4 -0.1281972554934967 1103 5 -0.3171272863360599 1103 6 -0.17747497346605456 1103 7 -0.2058499812249579 1103 8 -0.06474012481671565 1103 9 -0.6635220637922288 1103 10-11 -0.8198966892760637 1103 12-13 -0.19172765204746867 1103 14-15 -1.1362584522355448 1103 16-17 -0.9896416286526843 1103 18-19 -0.4407318333149135 1103 20-21 -0.5111618701870384 1103 22-23 -0.9049669064526142 1103 24-25 -0.5996731380944951 1103 26-27 0.011367600338417105 1103 28-29 0.06732328145676547 1103 30-31 0.5287828877639456 1103 32-33 0.4366861089850502 1103 34-35 0.8125262980706864 1103 36-37 1.2732271649624423 1103 38-39 1.7803164971359706 1103 40-41 1.428730417614652 1103 42-43 1.761605121891094 1103 44-45 2.4867298004044116 1103 46-47 1.4056468677099723 1103 48-49 1.8379213067776625 1103 50-51 2.9870584422870685 1103 52-53 2.949539629840629 1103 54-55 2.9290274161837146 1103 56-57 3.1766871751485404 1103 58-59 3.040104185596256 1103 60-61 2.528921952405316 1103 62-63 2.2754655599849727 1103 64-65 2.00225560233282 1103 66-67 2.8621602635906775 1103 68-69 3.1265758878074195 1103 70-71 2.3186457715795754 1103 72-73 2.3989846990905086 1103 74-75 2.604139409872019 1103 76-77 2.7560163179497117 1103 78-79 2.590708318177569 1103 80-81 3.755012868707393 1103 82-83 3.421669662167364 1103 84-85 3.3590109156009795 1103 86-87 3.6350292185673787 1103 88-89 3.2015013974451634 1103 90-91 3.986143019694442 1103 92-93 2.8857036084237073 1103 94-95 2.173623125685303 1103 96-97 2.2749680460242327 1103 98-99 2.270227302730655 1103 100-101 1.8255232540756818 1104 1 0.249557380162841 1104 2 0.4321586765128771 1104 3 0.11831079091214747 1104 4 0.6318027445065013 1104 5 0.4289596701856837 1104 6 0.5477424178382932 1104 7 0.6411065405141727 1104 8 0.49699900561806487 1104 9 0.6556083709903788 1104 10-11 0.8557554846369868 1104 12-13 1.059576695778624 1104 14-15 1.470263286894891 1104 16-17 0.6377496756951402 1104 18-19 0.3362246884242168 1104 20-21 0.16318595589991247 1104 22-23 0.281554832677827 1104 24-25 0.08119642712289732 1104 26-27 0.7231067307731962 1104 28-29 0.6577580640654688 1104 30-31 0.18182636602480784 1104 32-33 -0.6015747605801707 1104 34-35 -0.3566041367119226 1104 36-37 -0.4363380524288658 1104 38-39 -0.4892487202553326 1104 40-41 0.3609043306581299 1104 42-43 0.2229094697171803 1104 44-45 -0.08892237350863041 1104 46-47 -0.4147879148987208 1104 48-49 0.1474865241689649 1104 50-51 0.826623659678372 1104 52-53 1.453452673318889 1104 54-55 0.41381002487936414 1104 56-57 -0.9598345639818966 1104 58-59 -0.5907653796211356 1104 60-61 -0.9071650041164219 1104 62-63 -0.7184474834932892 1104 64-65 -0.8503530933193524 1104 66-67 -0.9008832146701913 1104 68-69 -0.43081541654040834 1104 70-71 -0.18918031537694446 1104 72-73 -1.1327544313442708 1104 74-75 -1.0402084162149379 1104 76-77 -0.9092010733546374 1104 78-79 -0.11885689921373555 1104 80-81 0.14414330349000082 1104 82-83 0.2529740099934479 1104 84-85 0.45596743734011014 1104 86-87 0.7506813924804234 1104 88-89 0.7201970496190775 1104 90-91 0.9248386718683541 1104 92-93 0.8652688258150114 1104 94-95 1.073188343076609 1104 96-97 1.2475767416764025 1104 98-99 1.449792520121962 1104 100-101 1.5029145584235106 1105 1 0.4903600557481269 1105 2 0.45473392734900386 1105 3 0.47449808188204656 1105 4 0.5548796675834211 1105 5 0.5259496367408616 1105 6 0.23252502653394203 1105 7 0.1503038649288868 1105 8 0.4367983367217434 1105 9 0.5510933208231563 1105 10-11 0.40048792610855344 1105 12-13 0.45673388641406376 1105 14-15 0.8118184708413736 1105 16-17 0.49728144827039245 1105 18-19 -0.02957798716107618 1105 20-21 0.29153043750526564 1105 22-23 0.17118693970123644 1105 24-25 0.21455763113628024 1105 26-27 0.7975214464922615 1105 28-29 0.6330925122259927 1105 30-31 1.201475195456247 1105 32-33 1.3382245705235079 1105 34-35 0.7671416826860735 1105 36-37 0.9813040880393586 1105 38-39 1.1976241894436654 1105 40-41 0.9971919560761862 1105 42-43 1.6104512757372476 1105 44-45 1.6644221080967228 1105 46-47 3.2541084061715075 1105 48-49 4.0663828452392 1105 50-51 3.7070584422870674 1105 52-53 3.221847322148321 1105 54-55 4.3251812623375585 1105 56-57 3.688994867456234 1105 58-59 2.131642647134715 1105 60-61 3.11122964471301 1105 62-63 1.9458501753695856 1105 64-65 0.961101756178973 1105 66-67 1.1179294943599096 1105 68-69 1.392345118576653 1105 70-71 1.5432611561949656 1105 72-73 2.081292391398204 1105 74-75 1.994908640641249 1105 76-77 2.1560163179497103 1105 78-79 1.6641698566391092 1105 80-81 1.7511667148612382 1105 82-83 1.4289773544750553 1105 84-85 1.0709339925240542 1105 86-87 0.7088753724135337 1105 88-89 1.2653475512913168 1105 90-91 1.5949891735405934 1105 92-93 1.5918574545775535 1105 94-95 1.2901615872237642 1105 96-97 1.4841988152550005 1105 98-99 2.301381148884504 1105 100-101 2.6605232540756827 1106 1 -0.1264490610609883 1106 2 -0.5388558162407442 1106 3 -0.09102898364501755 1106 4 -0.34967873697497964 1106 5 -0.823053212261982 1106 6 -0.7211786771697604 1106 7 -0.0754796108545861 1106 8 -0.37659197666857125 1106 9 -0.6761146563848257 1106 10-11 -1.2658226152019836 1106 12-13 -0.9058017261215454 1106 14-15 -1.3844066003836915 1106 16-17 -0.7185305175415735 1106 18-19 -0.06554664812972533 1106 20-21 -0.04856927759444574 1106 22-23 0.019477537991832605 1106 24-25 0.15366019523883523 1106 26-27 -0.0023361033652875562 1106 28-29 0.16621217034565205 1106 30-31 0.4632273322083833 1106 32-33 0.42298240528133846 1106 34-35 0.06771148325587006 1106 36-37 -0.6130691313338588 1106 38-39 0.7969831638026434 1106 40-41 1.5328044916887293 1106 42-43 0.36864215892813235 1106 44-45 0.48635943003404947 1106 46-47 0.26194316400627216 1106 48-49 -0.8054120265556755 1106 50-51 -1.0892378540092302 1106 52-53 -0.49823814793715115 1106 54-55 -0.12282443566813939 1106 56-57 0.1170575455189109 1106 58-59 -0.12619211070004255 1106 60-61 -0.8681150846317216 1106 62-63 -1.035275180755768 1106 64-65 -1.2807073606301422 1106 66-67 -1.3489508475204346 1106 68-69 -1.1300907788592447 1106 70-71 -1.0080208950870855 1106 72-73 -1.370274560168749 1106 74-75 -2.217342071609462 1106 76-77 -2.149539237605847 1106 78-79 -2.7585509410816904 1106 80-81 -3.5116537979592763 1106 82-83 -3.2124044119067126 1106 84-85 -3.242100195510133 1106 86-87 -3.0375633740252113 1106 88-89 -2.5736837877400216 1106 90-91 -2.4431162395648194 1106 92-93 -2.2450371323170337 1106 94-95 -3.7500805780184 1106 96-97 -3.312809731753548 1106 98-99 -3.0679208454174915 1106 100-101 -3.492254523702096 1107 1 0.22634441037654796 1107 2 -0.2026976241503462 1107 3 -0.10829200938261607 1107 4 0.21654850721836283 1107 5 0.20456762891817704 1107 6 0.21981316212715996 1107 7 0.20669239165639652 1107 8 -0.024740124816716502 1107 9 0.026647427733195173 1107 10-11 -0.08142211300487645 1107 12-13 -0.2766429062847564 1107 14-15 -0.045580486133850684 1107 16-17 0.1449346425337552 1107 18-19 0.3467257938037278 1107 20-21 0.24866863828753338 1107 22-23 0.2784229240558602 1107 24-25 -0.2813680533487286 1107 26-27 0.09967268508417959 1107 28-29 0.34935717976184577 1107 30-31 0.4467489894588539 1107 32-33 0.39787254966301333 1107 34-35 0.6579500268842438 1107 36-37 0.9035661480132902 1107 38-39 0.7192995479834323 1107 40-41 1.132459231173975 1107 42-43 1.611266138840243 1107 44-45 1.4287636987094956 1107 46-47 0.12242652872692261 1107 48-49 -0.24750242203589679 1107 50-51 -0.12768732042479414 1107 52-53 -0.7713078277864938 1107 54-55 -0.21588783805357536 1107 56-57 -1.1219568926480683 1107 58-59 -0.45277717033594556 1107 60-61 -0.8141288950523098 1107 62-63 0.002075729476498367 1107 64-65 0.8263233989429892 1107 66-67 2.147753483929659 1107 68-69 3.14250809119725 1107 70-71 2.319662720732122 1107 72-73 2.494916902480341 1107 74-75 2.7938004268211714 1107 76-77 2.6687281823564923 1107 78-79 2.7973184876690915 1107 80-81 3.0363688009107825 1107 82-83 3.0820086452182096 1107 84-85 3.0342651528891125 1107 86-87 2.5931648117877195 1107 88-89 2.670823431343468 1107 90-91 3.248346409524949 1107 92-93 3.4279069982542154 1107 94-95 3.2629451595836088 1107 96-97 2.803951096871687 1107 98-99 1.8217527264594704 1107 100-101 1.7939978303468695 1108 1 -0.7468194314313585 1108 2 -0.5888558162407413 1108 3 -0.29473268734871994 1108 4 -0.851530588826833 1108 5 -0.3304606196693882 1108 6 -0.6174749734660594 1108 7 -0.42918331455829417 1108 8 -0.6414067914833836 1108 9 -0.9001887304589005 1108 10-11 -1.4315633559427283 1108 12-13 -0.9567276520474692 1108 14-15 -1.5112584522355519 1108 16-17 -1.1046416286526863 1108 18-19 -1.10906516664825 1108 20-21 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76-77 2.0598624717958636 2316 78-79 1.660964728433978 2316 80-81 1.3537308174253404 2316 82-83 1.3167978672955662 2316 84-85 1.593369889959952 2316 86-87 1.5357984493366104 2316 88-89 1.5025270384708058 2316 90-91 0.43152763507905334 2316 92-93 0.835447198167298 2316 94-95 1.5754179974801765 2316 96-97 1.7854808665370516 2316 98-99 1.2725349950383507 2316 100-101 1.4329591515115805 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 3.0 5 3.0 6 10.0 7 40.0 8 86.0 9 146.0 10 274.0 11 426.0 12 713.0 13 1042.0 14 1282.0 15 1594.0 16 2071.0 17 2594.0 18 2582.0 19 2143.0 20 1998.0 21 2075.0 22 2104.0 23 1941.0 24 2219.0 25 2252.0 26 1617.0 27 810.0 28 690.0 29 800.0 30 887.0 31 1095.0 32 1128.0 33 1420.0 34 2149.0 35 4956.0 36 4280.0 37 6681.0 38 4165.0 39 250.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.13973276834228 23.586098486142912 14.959163448723645 14.315005296791169 2 15.304954115897946 26.22656663875455 38.643471128048255 19.825008117299248 3 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13.755424939343197 30.811946827051223 38.25393842053105 17.17868981307453 48-49 13.054027269931312 29.600519427263094 38.22830878583877 19.117144516966817 50-51 10.480811946827052 29.755151556573146 41.1449612138195 18.619075282780305 52-53 11.769128250692 30.290810921641665 40.78102040118922 17.15904042647712 54-55 10.966920684823839 29.1323514335509 38.31288658032327 21.587841301301985 56-57 14.79940539247514 30.318149198646754 34.136964767795504 20.745480641082594 58-59 12.543997539555068 32.97508799507911 36.37186891296176 18.10904555240406 60-61 13.345350784266822 36.17281208351844 32.087448313570036 18.394388818644707 62-63 10.601271229880735 38.35560263814373 31.39288521340943 19.65024091856611 64-65 10.651676178108875 38.44701500187951 31.043467860438096 19.857840959573522 66-67 11.544441786556401 36.029286129241704 30.54368998393876 21.882582100263132 68-69 11.767419608379182 34.209582066090285 31.5782729043502 22.44472542118033 70-71 13.312032259166868 32.06865324812904 31.21433209171992 23.404982400984178 72-73 12.954071694631446 28.845299524997436 33.32706831151967 24.873560468851448 74-75 12.602945699347298 28.916208180979392 32.239517479410864 26.241328640262445 76-77 12.638827187916482 27.160578204558654 34.0797252503161 26.120869357208765 78-79 13.071113693059495 26.256706421077812 34.92379455284831 25.748385333014383 80-81 13.620442196630558 25.942316235519257 33.56542391415781 26.871817653692375 82-83 12.787479069131669 25.779140894645113 34.0985203157571 27.33485972046612 84-85 13.320631557363043 25.742455828577288 32.791087112538875 28.145825501520793 86-87 13.363291528551413 25.82783720056043 32.02593719030858 28.78293408057957 88-89 13.568328606089603 25.195639544817688 30.89310733691009 30.342924512182616 90-91 13.962170659194204 23.697160236476094 31.742302566380754 30.598366537948944 92-93 13.981939496458809 24.19885349121323 31.435869834516577 30.38333717781138 94-95 13.41540511909237 23.621125653555687 31.337354338242836 31.626114889109115 96-97 12.46539999316543 23.85008372347333 31.40826299422479 32.276253289136456 98-99 13.426511294125687 24.353278884598296 29.843146635683286 32.37706318559273 100-101 13.246249530123363 23.942350408365513 30.217339302190478 32.59406075932064 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1953.0 1 1092.5 2 317.0 3 410.5 4 288.5 5 212.5 6 394.5 7 648.5 8 819.5 9 852.5 10 631.5 11 367.5 12 307.0 13 296.0 14 288.5 15 278.5 16 289.0 17 319.5 18 351.5 19 377.0 20 422.5 21 485.0 22 502.5 23 537.5 24 619.0 25 696.0 26 784.5 27 850.0 28 919.5 29 1060.0 30 1177.5 31 1222.0 32 1286.5 33 1285.0 34 1300.5 35 1341.0 36 1267.0 37 1173.0 38 1103.0 39 1067.5 40 1029.5 41 1057.5 42 1101.0 43 1088.5 44 1108.0 45 1180.0 46 1329.0 47 1977.5 48 3494.0 49 3619.0 50 3002.0 51 3196.5 52 2820.5 53 1748.0 54 686.5 55 310.0 56 227.5 57 180.0 58 155.5 59 129.0 60 100.5 61 78.0 62 66.0 63 52.0 64 39.0 65 31.5 66 23.0 67 16.5 68 15.5 69 10.0 70 7.0 71 6.0 72 4.5 73 7.5 74 6.0 75 2.0 76 0.5 77 1.0 78 1.0 79 0.5 80 0.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.01537778081536411 3 0.013669138502545877 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.005980248094863821 26-27 0.02904691931790999 28-29 0.0 30-31 0.0 32-33 8.543211564091173E-4 34-35 0.023920992379455285 36-37 0.005125926938454704 38-39 0.02562963469227352 40-41 0.01879506544100058 42-43 0.011106175033318524 44-45 0.004271605782045587 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.006834569251272939 86-87 0.0 88-89 0.0 90-91 0.0 92-93 8.543211564091173E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 58526.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.49400266548201 #Duplication Level Percentage of deduplicated Percentage of total 1 85.41435483155044 32.879404025561286 2 7.3061387544942065 5.624850493797629 3 2.423542989036353 2.798756108396268 4 1.3538106440587687 2.0845436216382462 5 0.6302987260863775 1.2131360421009465 6 0.4749434062763549 1.0969483648293068 7 0.31958808646633224 0.8611557256603902 8 0.24412978827289272 0.7518026176400233 9 0.18642638377202717 0.6458667942452927 >10 1.3094234098273336 9.956258756791852 >50 0.1331617026943051 3.6052352800464753 >100 0.15535531981002265 11.999794962922463 >500 0.02219361711571752 5.838430782899907 >1k 0.026632340538861023 20.64381642346991 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT 2537 4.334825547619861 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2302 3.933294604107576 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2165 3.6992106072514783 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2102 3.591566141543929 No Hit ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT 1541 2.6330178040528995 RNA PCR Primer, Index 31 (95% over 22bp) TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1435 2.4519017188941667 No Hit ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT 855 1.4608891774595905 RNA PCR Primer, Index 31 (95% over 24bp) GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 730 1.2473088883573114 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 727 1.2421829614188564 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 600 1.0251853876909407 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 505 0.8628643679732085 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 449 0.7671803984553873 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 418 0.7142124867580221 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 398 0.6800396405016573 No Hit TTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC 352 0.6014420941120185 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 346 0.5911902402351091 No Hit CATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCT 331 0.5655606055428357 RNA PCR Primer, Index 31 (96% over 27bp) CACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTG 243 0.415200082014831 RNA PCR Primer, Index 38 (95% over 24bp) GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 237 0.4049482281379216 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 233 0.3981136588866486 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 223 0.3810272357584663 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 208 0.3553976010661928 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 203 0.3468543895021016 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 192 0.32805932406110105 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 191 0.3263506817482828 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 189 0.32293339712264635 No Hit TATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCT 188 0.32122475480982815 RNA PCR Primer, Index 31 (95% over 21bp) TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 177 0.3024296893688275 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 175 0.2990124047431911 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 171 0.2921778354919181 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 170 0.2904691931790999 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 164 0.2802173393021905 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 157 0.26825684311246284 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 156 0.26654820079964464 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 145 0.24775313535864404 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 142 0.2426272084201893 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 131 0.22383214297918874 No Hit CTTTACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC 129 0.2204148583535523 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 127 0.2169975737279158 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 118 0.2016197929125517 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 118 0.2016197929125517 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 117 0.19991115059973344 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 109 0.18624201209718758 No Hit ACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGC 108 0.18453336978436935 RNA PCR Primer, Index 38 (96% over 25bp) GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 106 0.18111608515873287 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 102 0.17428151590745994 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 97 0.16573830434336875 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 94 0.16061237740491408 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 92 0.1571950927792776 No Hit GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 92 0.1571950927792776 No Hit CTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC 89 0.1520691658408229 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 86 0.14694323890236818 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 85 0.14523459658954996 No Hit GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACGCTACGC 81 0.138400027338277 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 78 0.13327410039982232 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 77 0.13156545808700407 No Hit GGTATCAACGCAGAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 74 0.12643953114854936 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 73 0.12473088883573112 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 69 0.1178963195844582 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 67 0.11447903495882172 No Hit TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG 66 0.11277039264600348 RNA PCR Primer, Index 31 (96% over 29bp) CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 66 0.11277039264600348 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 66 0.11277039264600348 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 65 0.11106175033318524 No Hit GTATCAACGCAGAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 64 0.10935310802036702 No Hit GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA 64 0.10935310802036702 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 61 0.10422718108191233 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 60 0.10251853876909409 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 60 0.10251853876909409 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 59 0.10080989645627585 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 59 0.10080989645627585 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 8.543211564091173E-4 0.0 20-21 0.0 0.0 0.0 0.013669138502545877 0.0 22-23 0.0 0.0 0.0 0.04100741550763763 0.0 24-25 0.0 0.0 0.0 0.09568396951782114 0.0 26-27 0.0 0.0 0.0 0.22041485835355226 0.0 28-29 0.0 0.0 0.0 0.5442025766326077 0.0 30-31 0.0 0.0 0.0 1.0602125551037145 0.0 32-33 0.0 0.0 0.0 1.740252195605372 0.0 34-35 0.0 0.0 0.0 2.7961931449270407 0.0 36-37 0.0 0.0 0.0 3.9973686908382597 0.0 38-39 0.0 0.0 0.0 5.301917096674982 0.0 40-41 0.0 0.0 0.0 6.49454943102211 0.0 42-43 0.0 0.0 0.0 7.348870587431227 0.0 44-45 0.0 0.0 0.0 8.122885555137888 0.0 46-47 0.0 0.0 0.0 8.926801763318867 0.0 48-49 0.0 0.0 0.0 9.666643884769162 0.0 50-51 0.0 0.0 0.0 10.21938967296586 0.0 52-53 0.0 0.0 0.0 10.711478659057512 0.0 54-55 0.0 0.0 0.0 11.241157776031166 0.0 56-57 0.0 0.0 0.0 11.83576530089191 0.0 58-59 0.0 0.0 0.0 12.251819704063152 0.0 60-61 0.0 0.0 0.0 12.678980282267709 0.0 62-63 0.0 0.0 0.0 13.071113693059495 0.0 64-65 0.0 0.0 0.0 13.397464374807777 0.0 66-67 0.0 0.0 0.0 13.757987902812424 0.0 68-69 0.0 0.0 0.0 14.20906947339644 0.0 70-71 0.0 0.0 0.0 14.649044868947135 0.0 72-73 0.0 0.0 0.0 15.06937087790042 0.0 74-75 0.0 0.0 0.0 15.414516625089703 0.0 76-77 0.0 0.0 0.0 15.67850186242012 0.0 78-79 0.0 0.0 0.0 16.09711922906059 0.0 80-81 0.0 0.0 0.0 16.458497078221647 0.0 82-83 0.0 0.0 0.0 16.778867511875063 0.0 84-85 0.0 0.0 0.0 17.134265112941257 0.0 86-87 0.0 0.0 0.0 17.486245429381814 0.0 88-89 0.0 0.0 0.0 17.816867716912142 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTACA 20 0.00206422 71.25 1 ACGCAGG 20 0.00206422 71.25 7 GTGTATA 35 2.766073E-4 54.28571 6 TATAAGA 35 2.766073E-4 54.28571 9 GATGTGT 35 2.766073E-4 54.28571 3 ATGTGTA 35 2.766073E-4 54.28571 4 AGATGTG 35 2.766073E-4 54.28571 2 GAGATGT 35 2.766073E-4 54.28571 1 GTATAAG 40 5.346096E-4 47.500004 8 TGTATAA 40 5.346096E-4 47.500004 7 TGTGTAT 40 5.346096E-4 47.500004 5 GTACCCA 50 2.7939639E-5 47.5 1 ACCCATG 55 4.9055307E-5 43.18182 3 ATAAGAG 35 2.0421202E-4 33.92857 10-11 TACCCAT 70 2.0296895E-4 33.92857 2 TAAGAGA 30 0.0039365897 31.666668 10-11 GACACAT 40 4.469282E-4 29.687502 20-21 GGGCTTG 130 2.9544753E-7 29.23077 7 GGCTTGA 135 4.1107523E-7 28.148148 8 GAGTGCA 35 0.00836134 27.142855 12-13 >>END_MODULE