FastQCFastQC Report
Thu 26 May 2016
SRR937987_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937987_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2006473
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82480.4110695733259306No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68860.3431892679343305No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42210.21036914027749187No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT23760.11841674420737285No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69400.032.227581
GTATCAA98850.030.2968391
TATCAAC118000.025.2146242
ATCAACG117750.025.10673
TCAACGC118050.025.0831584
CAACGCA119350.024.7302975
AACGCAG122100.024.2061966
GAGTACT76450.022.86553412-13
GTGTAGC11800.022.6318661
ACGCAGA133750.021.984067
TAGCCCT13900.021.8839324
CGCAGAG135100.021.7292238
GTACTTT81450.021.6395414-15
AGAGTAC104950.021.06947510-11
AGTACTT80300.020.34951212-13
GCAGAGT143250.020.0618889
GTAAGGT11250.019.4341474
GTACATG57450.018.9260141
TAAGGTG12450.018.3244975
GTAGCCC10650.018.2976113
Produced by FastQC (version 0.11.5)