##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937987_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2006473 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.896230848857673 33.0 31.0 34.0 30.0 34.0 2 32.065907689762085 33.0 31.0 34.0 30.0 34.0 3 32.15388096425917 34.0 31.0 34.0 30.0 34.0 4 35.68189753861627 37.0 35.0 37.0 35.0 37.0 5 35.69476838213123 37.0 35.0 37.0 35.0 37.0 6 35.73123435999388 37.0 35.0 37.0 35.0 37.0 7 35.66449286883003 37.0 35.0 37.0 33.0 37.0 8 35.635079066600944 37.0 35.0 37.0 33.0 37.0 9 37.27217809559361 39.0 37.0 39.0 34.0 39.0 10-11 37.16527683153474 39.0 37.0 39.0 34.0 39.0 12-13 37.027325311628914 39.0 37.0 39.0 33.0 39.0 14-15 38.16983258683271 40.0 38.0 41.0 33.0 41.0 16-17 37.97745820651461 40.0 38.0 41.0 33.0 41.0 18-19 38.1794601771367 40.0 38.0 41.0 33.5 41.0 20-21 38.16237472420511 40.0 38.0 41.0 33.5 41.0 22-23 38.04627672537831 40.0 38.0 41.0 33.0 41.0 24-25 37.9042132637718 40.0 38.0 41.0 33.0 41.0 26-27 37.68697435749198 40.0 38.0 41.0 32.0 41.0 28-29 37.46457365735796 40.0 37.0 41.0 32.0 41.0 30-31 37.196102813244934 40.0 37.0 41.0 31.0 41.0 32-33 36.84852749077611 39.0 36.0 41.0 30.5 41.0 34-35 36.53167373794713 39.0 35.5 41.0 30.0 41.0 36-37 36.19762638221397 39.0 35.0 40.5 29.5 41.0 38-39 35.84211823433458 38.5 35.0 40.0 28.5 41.0 40-41 35.648648648648646 39.0 35.0 40.0 27.0 41.0 42-43 35.34317930019492 38.0 35.0 40.0 26.5 41.0 44-45 34.945080247778066 38.0 34.0 40.0 24.5 41.0 46-47 34.45773877844357 38.0 33.5 40.0 23.5 41.0 48-49 33.9340300118666 38.0 33.0 40.0 21.0 41.0 50-51 33.08972037002242 37.5 32.0 39.5 18.5 40.5 52-53 33.04449225083019 37.5 32.5 39.5 17.0 40.5 54-55 32.97583047466873 37.5 32.0 40.0 13.5 41.0 56-57 32.45290193289419 37.0 31.0 40.0 8.0 41.0 58-59 31.936574028157867 36.5 31.0 40.0 4.5 41.0 60-61 31.313003215094348 36.0 30.0 39.0 2.0 41.0 62-63 30.641897997132283 35.0 29.0 39.0 2.0 40.5 64-65 29.989247301109955 35.0 28.0 39.0 2.0 40.0 66-67 29.240950663178623 34.5 27.0 38.0 2.0 40.0 68-69 28.479937930886685 34.0 26.0 37.5 2.0 40.0 70-71 27.57407002237259 34.0 24.0 37.0 2.0 39.0 72-73 26.72038123612927 33.0 21.0 36.0 2.0 39.0 74-75 25.843877789534172 32.5 17.5 35.5 2.0 38.5 76-77 24.953509964998283 31.5 9.5 35.0 2.0 37.5 78-79 24.07361399829452 31.0 4.0 35.0 2.0 37.0 80-81 23.22536435825451 30.5 2.0 35.0 2.0 36.0 82-83 22.3515215505018 30.0 2.0 34.0 2.0 36.0 84-85 21.502678082386353 29.0 2.0 34.0 2.0 35.0 86-87 20.70251755194314 28.5 2.0 34.0 2.0 35.0 88-89 19.833761281612063 26.5 2.0 34.0 2.0 35.0 90-91 18.956089366764466 25.5 2.0 34.0 2.0 35.0 92-93 18.039451565009845 24.0 2.0 33.0 2.0 35.0 94-95 17.090344350509575 19.0 2.0 32.5 2.0 35.0 96-97 15.995165147998502 4.5 2.0 32.0 2.0 34.5 98-99 14.708105466657162 2.0 2.0 32.0 2.0 34.0 100-101 11.837065836420425 2.0 2.0 27.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4672961068129773 1101 2 0.5205221082000477 1101 3 0.4660245799780043 1101 4 0.298252545964246 1101 5 0.3581041069334532 1101 6 0.32507817238774095 1101 7 0.3079322084017164 1101 8 0.32120221028647933 1101 9 0.43204701421313985 1101 10-11 0.43924688495444286 1101 12-13 0.4023040108310454 1101 14-15 0.5851472652624139 1101 16-17 0.7021427594598961 1101 18-19 0.7152654738173538 1101 20-21 0.5933334807568755 1101 22-23 0.7801526489801844 1101 24-25 0.8159751018278527 1101 26-27 0.9242055302052989 1101 28-29 0.9310616210105422 1101 30-31 0.8685488351615689 1101 32-33 0.9430223140934686 1101 34-35 1.1472196786830224 1101 36-37 1.1589810327026626 1101 38-39 1.438186812954207 1101 40-41 1.4990422673138752 1101 42-43 1.7552809045939313 1101 44-45 1.8969360748297248 1101 46-47 1.979661276104835 1101 48-49 2.011561500162685 1101 50-51 2.2928065185881437 1101 52-53 2.5406222166776544 1101 54-55 2.4915678904185796 1101 56-57 2.671028089854893 1101 58-59 2.9740123937900087 1101 60-61 3.215135215304059 1101 62-63 3.370344738454076 1101 64-65 3.474258136972683 1101 66-67 3.53058401990792 1101 68-69 3.9415925986524414 1101 70-71 3.9873649517314966 1101 72-73 4.314323249731807 1101 74-75 4.487652064182576 1101 76-77 4.76323757135356 1101 78-79 4.875573296758592 1101 80-81 5.190469767316827 1101 82-83 5.383420430421573 1101 84-85 5.546159218107839 1101 86-87 5.690349256957212 1101 88-89 5.793846606862683 1101 90-91 5.987939870296685 1101 92-93 6.3682751539387645 1101 94-95 6.25101672284929 1101 96-97 6.1035705231006006 1101 98-99 6.01261235860099 1101 100-101 5.005058796929029 1102 1 0.4785303686722848 1102 2 0.44568473147612764 1102 3 0.4338947974912486 1102 4 0.2592468467033271 1102 5 0.35786199303434074 1102 6 0.342066546791969 1102 7 0.3051235692115881 1102 8 0.33643621735113527 1102 9 0.4910591069324397 1102 10-11 0.4540125888865916 1102 12-13 0.5564447483082162 1102 14-15 0.7718595472493206 1102 16-17 0.8415615858356276 1102 18-19 0.7480716122451625 1102 20-21 0.7747847250335838 1102 22-23 0.7175872914979848 1102 24-25 0.6683072986119001 1102 26-27 0.8213913173821723 1102 28-29 0.8587345908958213 1102 30-31 0.9807220330054918 1102 32-33 1.0211182018139482 1102 34-35 1.0828877154607426 1102 36-37 1.3324189262114814 1102 38-39 1.374282898848314 1102 40-41 1.2956580399285258 1102 42-43 1.5007397207542823 1102 44-45 1.6505589362798219 1102 46-47 1.6997905843904348 1102 48-49 1.8463342463358998 1102 50-51 2.087862853567607 1102 52-53 2.203943818840578 1102 54-55 2.1491746018984372 1102 56-57 2.5092629763149787 1102 58-59 2.5304625321871477 1102 60-61 2.682599501292188 1102 62-63 2.9067482293767277 1102 64-65 2.953833868489898 1102 66-67 3.239628138899384 1102 68-69 3.5941767000559963 1102 70-71 3.9767260954291928 1102 72-73 4.192753319511333 1102 74-75 4.420180140252935 1102 76-77 4.4343609326612246 1102 78-79 4.542251759473931 1102 80-81 4.966064391385604 1102 82-83 5.145795075286781 1102 84-85 5.192293387545099 1102 86-87 5.595119166180719 1102 88-89 5.608580667894259 1102 90-91 5.833678370771185 1102 92-93 5.681931351022428 1102 94-95 5.590687040982825 1102 96-97 5.675511822423566 1102 98-99 5.671437012106804 1102 100-101 4.469879293507292 1103 1 0.48569212419377195 1103 2 0.4268980398279112 1103 3 0.41643780549669884 1103 4 0.28927409930084025 1103 5 0.31278051912303084 1103 6 0.3112518076788078 1103 7 0.3133277365879579 1103 8 0.19148248999066197 1103 9 0.2846995997502475 1103 10-11 0.3353241201419124 1103 12-13 0.452854786348297 1103 14-15 0.7201511500427102 1103 16-17 0.7156534497997384 1103 18-19 0.7405371206764357 1103 20-21 0.6718630194751896 1103 22-23 0.6482004749420369 1103 24-25 0.7851939732272442 1103 26-27 0.8492403493471983 1103 28-29 0.928341699281674 1103 30-31 0.9454710820033867 1103 32-33 1.0640870316555464 1103 34-35 1.073203391381945 1103 36-37 1.1324510047898002 1103 38-39 1.2656514902296792 1103 40-41 1.2876469117845417 1103 42-43 1.3764014478876518 1103 44-45 1.5066601115480864 1103 46-47 1.6060297259336096 1103 48-49 1.775676777420891 1103 50-51 1.8939211627710435 1103 52-53 1.9967627593958497 1103 54-55 2.2204710874488853 1103 56-57 2.4576851644714495 1103 58-59 2.832459920479888 1103 60-61 2.8780761378674455 1103 62-63 3.056343414751165 1103 64-65 3.2538049126050375 1103 66-67 3.4493485158936465 1103 68-69 3.550650611371495 1103 70-71 3.853124210457864 1103 72-73 4.094223540370926 1103 74-75 4.378407869770907 1103 76-77 4.581377624877199 1103 78-79 4.919869144360096 1103 80-81 5.126198667204601 1103 82-83 5.1692819883623535 1103 84-85 5.262679749891092 1103 86-87 5.274138356551468 1103 88-89 5.381629836122272 1103 90-91 5.540538644432683 1103 92-93 5.889545045452678 1103 94-95 5.611230673526968 1103 96-97 5.511895175628581 1103 98-99 5.423417083644871 1103 100-101 4.004567875804458 1104 1 0.44724078589468164 1104 2 0.39247960649730373 1104 3 0.41456036695477394 1104 4 0.3494364413924629 1104 5 0.33177154256031827 1104 6 0.3048530736742805 1104 7 0.3303122663415792 1104 8 0.3512568481255016 1104 9 0.35254140488516583 1104 10-11 0.5361895808387587 1104 12-13 0.5171861824902848 1104 14-15 0.6974636381057948 1104 16-17 0.7474667700365742 1104 18-19 0.6967534585662563 1104 20-21 0.6237339097646739 1104 22-23 0.6098650952798081 1104 24-25 0.7671910537980295 1104 26-27 0.8832671153290264 1104 28-29 0.793748373042277 1104 30-31 0.936395613394069 1104 32-33 1.0509326838887745 1104 34-35 1.1186629968497286 1104 36-37 1.2218195076955851 1104 38-39 1.3578802152303595 1104 40-41 1.4390124952611174 1104 42-43 1.4288540412684654 1104 44-45 1.4614084130951142 1104 46-47 1.7533673156310243 1104 48-49 1.9675877757565416 1104 50-51 2.0010168763941323 1104 52-53 1.977736266257871 1104 54-55 1.9875820803276554 1104 56-57 2.479659462580642 1104 58-59 2.7084002768139577 1104 60-61 2.8870974469722483 1104 62-63 2.7010696159462206 1104 64-65 2.9792003997677305 1104 66-67 3.1559190051026746 1104 68-69 3.201864084400885 1104 70-71 3.532000639477534 1104 72-73 3.7842234039489995 1104 74-75 4.191451033668205 1104 76-77 4.425783370968713 1104 78-79 4.638967531852934 1104 80-81 4.8248967927673405 1104 82-83 5.0129022170055 1104 84-85 5.149182028137254 1104 86-87 5.543523775776045 1104 88-89 5.254223899040067 1104 90-91 5.167564919295579 1104 92-93 5.458256676786068 1104 94-95 5.4332329381309314 1104 96-97 5.191189328584848 1104 98-99 4.920514707174924 1104 100-101 3.8944360922242023 1105 1 0.3971157132869898 1105 2 0.3713453025630642 1105 3 0.368380312366277 1105 4 0.22368225825243826 1105 5 0.23589191206320947 1105 6 0.21774726318504634 1105 7 0.24225396782870945 1105 8 0.23139794126946356 1105 9 0.3689460592725453 1105 10-11 0.34631968133405877 1105 12-13 0.40033893346306826 1105 14-15 0.5428244749211686 1105 16-17 0.6484509556124678 1105 18-19 0.5626064506278183 1105 20-21 0.6379103667590655 1105 22-23 0.6380165814734227 1105 24-25 0.7522701727622163 1105 26-27 0.7552464791294824 1105 28-29 0.8169477022408813 1105 30-31 0.833668608953289 1105 32-33 0.8438703755574721 1105 34-35 0.9838914066007121 1105 36-37 1.098323970336196 1105 38-39 1.007466117158451 1105 40-41 1.1269012977494484 1105 42-43 1.2905348234953493 1105 44-45 1.3999448293667314 1105 46-47 1.6207559994487326 1105 48-49 1.459914782071067 1105 50-51 1.578340947171803 1105 52-53 1.6585462089836724 1105 54-55 1.7921916539253147 1105 56-57 2.3523887155177405 1105 58-59 2.629802448486654 1105 60-61 2.909866985468369 1105 62-63 2.876724412426075 1105 64-65 2.939945791911736 1105 66-67 2.9546914666440216 1105 68-69 3.121235582836306 1105 70-71 3.5439797378305116 1105 72-73 3.674369915344503 1105 74-75 3.875199245801344 1105 76-77 3.9858143190222 1105 78-79 4.2354842363068315 1105 80-81 4.395916485847593 1105 82-83 4.4968871458343465 1105 84-85 4.309362797872495 1105 86-87 4.395525484008665 1105 88-89 4.59371131995233 1105 90-91 4.652003451027483 1105 92-93 4.871639211590914 1105 94-95 4.893196431294882 1105 96-97 4.950303545951982 1105 98-99 4.676851875443646 1105 100-101 3.739821521968061 1106 1 0.47169856670369015 1106 2 0.3064042028074425 1106 3 0.37236438218119616 1106 4 0.20014729899202166 1106 5 0.23439274391870413 1106 6 0.3160235245278358 1106 7 0.16665414821642344 1106 8 0.20454241598166334 1106 9 0.29793363324064615 1106 10-11 0.3051870171416269 1106 12-13 0.3504675968266966 1106 14-15 0.5269754186048559 1106 16-17 0.501210247712585 1106 18-19 0.45715200766629494 1106 20-21 0.577375358745222 1106 22-23 0.5288439272754459 1106 24-25 0.5285524642955792 1106 26-27 0.6965900019308577 1106 28-29 0.766287550252045 1106 30-31 0.8151533491279537 1106 32-33 0.7456603884161908 1106 34-35 0.7831392325021866 1106 36-37 0.9597088116742327 1106 38-39 1.0553800795329877 1106 40-41 1.214439234016524 1106 42-43 1.118294267949949 1106 44-45 1.2514951886186196 1106 46-47 1.2310972125560866 1106 48-49 1.5820817503996238 1106 50-51 1.8099860404432135 1106 52-53 1.5868668511883044 1106 54-55 1.7882906173053925 1106 56-57 1.8471625757538277 1106 58-59 2.2369049861029744 1106 60-61 2.370723689979897 1106 62-63 2.2428349090407558 1106 64-65 2.4806667181051303 1106 66-67 2.6569782313050894 1106 68-69 2.566840079731321 1106 70-71 3.0931875706793583 1106 72-73 3.391029040464101 1106 74-75 3.4798370687166766 1106 76-77 3.3929367388989746 1106 78-79 3.756164834267718 1106 80-81 3.8419510032799913 1106 82-83 4.062004215021563 1106 84-85 3.970864266569741 1106 86-87 4.115157045154614 1106 88-89 4.310144198659874 1106 90-91 4.349960682076105 1106 92-93 4.290526064008173 1106 94-95 4.2573762156828145 1106 96-97 4.168752455398568 1106 98-99 3.909470513882077 1106 100-101 2.9735873896878005 1107 1 0.30792768227233225 1107 2 0.35585189596285716 1107 3 0.3055295255518402 1107 4 0.2167044239568554 1107 5 0.3109347645208089 1107 6 0.2664768502544348 1107 7 0.1906964279620027 1107 8 0.2564209918693976 1107 9 0.29181472963968247 1107 10-11 0.3751035378695988 1107 12-13 0.4114910148239588 1107 14-15 0.5292134050639348 1107 16-17 0.5954028618506655 1107 18-19 0.4884152327516915 1107 20-21 0.602454212403579 1107 22-23 0.5871675768261682 1107 24-25 0.5733437583217196 1107 26-27 0.6356308826913448 1107 28-29 0.7006326732497214 1107 30-31 0.8900045072459974 1107 32-33 0.8533806818055751 1107 34-35 0.8962380195548363 1107 36-37 0.9359580196304407 1107 38-39 1.0203976584236685 1107 40-41 1.0466175278470402 1107 42-43 1.0801810818724604 1107 44-45 1.3013547835130623 1107 46-47 1.5865668545795728 1107 48-49 1.7917444118382093 1107 50-51 1.6861411773683912 1107 52-53 1.8349045700086677 1107 54-55 1.7934052912171623 1107 56-57 2.0899355902276753 1107 58-59 2.105778386135551 1107 60-61 2.250309263053289 1107 62-63 2.3597245949744483 1107 64-65 2.575033523423297 1107 66-67 2.7421916213460307 1107 68-69 2.911364124738892 1107 70-71 3.261931282066005 1107 72-73 3.4961497155932655 1107 74-75 3.6366820027149735 1107 76-77 3.655122522560543 1107 78-79 3.668538487208746 1107 80-81 3.645238891030729 1107 82-83 3.81363564986588 1107 84-85 3.9477116478177265 1107 86-87 4.159076993590908 1107 88-89 4.250446504140545 1107 90-91 4.327188010528971 1107 92-93 4.4167947954053695 1107 94-95 4.350284413423704 1107 96-97 4.412615041549163 1107 98-99 4.164351179284587 1107 100-101 3.4318941267167933 1108 1 0.3651189932532155 1108 2 0.3914986924027417 1108 3 0.28748476251187327 1108 4 0.23502385103867596 1108 5 0.19718398060692977 1108 6 0.19269882143962747 1108 7 0.258241908810966 1108 8 0.22378636288043197 1108 9 0.30304688305284344 1108 10-11 0.37901542387293574 1108 12-13 0.3016829889302315 1108 14-15 0.42229613514697917 1108 16-17 0.3864024922171083 1108 18-19 0.3473061659310801 1108 20-21 0.34625371319061315 1108 22-23 0.49076395912418747 1108 24-25 0.4507529177583791 1108 26-27 0.5019832916085107 1108 28-29 0.4124642698590577 1108 30-31 0.3248334028999693 1108 32-33 0.5077130779032402 1108 34-35 0.6275742241241673 1108 36-37 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1.7432088988637346 2311 50-51 1.7790125304306983 2311 52-53 1.692488941940816 2311 54-55 1.8884609989364662 2311 56-57 2.211507378233705 2311 58-59 2.301195525314757 2311 60-61 2.401427713007827 2311 62-63 2.506890193089415 2311 64-65 2.763373771461044 2311 66-67 2.9006647812405575 2311 68-69 3.088197009011168 2311 70-71 3.629273444301969 2311 72-73 3.9337514741293695 2311 74-75 4.210606879954593 2311 76-77 4.088773170703792 2311 78-79 4.347831455200488 2311 80-81 4.323937793501354 2311 82-83 4.734243442630937 2311 84-85 5.048333599705504 2311 86-87 4.97670236150055 2311 88-89 5.1447867791126605 2311 90-91 5.305574334924735 2311 92-93 5.3945250587771305 2311 94-95 5.208933157585214 2311 96-97 4.999514857745268 2311 98-99 5.051545950114843 2311 100-101 4.6489045085255025 2312 1 -0.21338417630266093 2312 2 -0.1516707670798354 2312 3 -0.12766331677702425 2312 4 -0.1299308979934679 2312 5 -0.03199232044124045 2312 6 -0.013114942903499127 2312 7 -0.07162156110867102 2312 8 -0.011699167774672503 2312 9 0.030574243893013886 2312 10-11 -0.06738251233108628 2312 12-13 -0.08003865646750086 2312 14-15 -0.0766293125128712 2312 16-17 0.08961458195512506 2312 18-19 -0.02878257440391252 2312 20-21 -0.031332990218238876 2312 22-23 0.09866067775124776 2312 24-25 0.058439979391508245 2312 26-27 0.10247176830586113 2312 28-29 0.23314512492221695 2312 30-31 0.27758587390042777 2312 32-33 0.3483996684106003 2312 34-35 0.49580697230563686 2312 36-37 0.5069912725356076 2312 38-39 0.6724833609211629 2312 40-41 0.7132427520781661 2312 42-43 0.8169778777867194 2312 44-45 0.9469217401390182 2312 46-47 1.032828069579942 2312 48-49 1.1175394694661591 2312 50-51 1.114229023392646 2312 52-53 1.3545370341529193 2312 54-55 1.5551040545335582 2312 56-57 1.7975938767976416 2312 58-59 1.735696633182517 2312 60-61 1.9387640893061615 2312 62-63 2.040675814817046 2312 64-65 2.2584142342999804 2312 66-67 2.55301872740813 2312 68-69 2.8060002337510923 2312 70-71 3.1412215212359236 2312 72-73 3.2634893569015233 2312 74-75 2.9981943554307406 2312 76-77 3.200362049506051 2312 78-79 3.6342487175666953 2312 80-81 3.844105756115969 2312 82-83 4.100212157997436 2312 84-85 4.365668597737127 2312 86-87 4.500522023586736 2312 88-89 4.761569722718516 2312 90-91 4.723123873710616 2312 92-93 4.871441617459357 2312 94-95 4.6969979134864985 2312 96-97 4.579300535250255 2312 98-99 4.277512676897114 2312 100-101 3.7531427999274616 2313 1 -0.43734483790301937 2313 2 -0.31483572014197136 2313 3 -0.29172232437649015 2313 4 -0.19206768833767995 2313 5 -0.2179556642052063 2313 6 -0.2464631771457917 2313 7 -0.11185401528837957 2313 8 -0.14446626656769723 2313 9 -0.1209680001838791 2313 10-11 -0.17176337956399124 2313 12-13 -0.15938599665525288 2313 14-15 -0.14480097098404343 2313 16-17 0.03977104149915078 2313 18-19 -0.10991802366631731 2313 20-21 -0.08497626245785028 2313 22-23 -0.061598598064584564 2313 24-25 -0.035435747027797504 2313 26-27 -0.04907047577103185 2313 28-29 0.07221530820339694 2313 30-31 0.15040661596569294 2313 32-33 0.12220387046692593 2313 34-35 0.3856147505801104 2313 36-37 0.2557619475467803 2313 38-39 0.392197263469221 2313 40-41 0.5431488763517436 2313 42-43 0.6544834656275782 2313 44-45 0.8235422139879205 2313 46-47 1.0124883288557598 2313 48-49 1.1615716554294977 2313 50-51 1.2937104717609955 2313 52-53 1.5530171414394687 2313 54-55 1.6668608716994058 2313 56-57 1.8051933403649159 2313 58-59 2.058673834791822 2313 60-61 2.3960729851488125 2313 62-63 2.4020973615313927 2313 64-65 2.7450034162400776 2313 66-67 2.8391161793527004 2313 68-69 2.9756470822088517 2313 70-71 3.3915567916874245 2313 72-73 3.7064933801469415 2313 74-75 3.9867951600798257 2313 76-77 4.082793877847575 2313 78-79 4.240641207508581 2313 80-81 4.5041415182974625 2313 82-83 4.830207687724748 2313 84-85 4.9108183518721304 2313 86-87 4.999851482683738 2313 88-89 5.095275578775734 2313 90-91 5.047218643491572 2313 92-93 5.0719333474548876 2313 94-95 5.081217850902682 2313 96-97 5.136743539273503 2313 98-99 5.094902037648119 2313 100-101 4.422119107482223 2314 1 -0.4138815280634951 2314 2 -0.35822954496233805 2314 3 -0.23130812046304428 2314 4 -0.20693396204941195 2314 5 -0.2709698802926823 2314 6 -0.23809954120510923 2314 7 -0.29825760143184965 2314 8 -0.2990351527719213 2314 9 -0.22174628512673422 2314 10-11 -0.3151213995549824 2314 12-13 -0.24908203749796343 2314 14-15 -0.33630979851255205 2314 16-17 -0.20665768362793102 2314 18-19 -0.17898654755746435 2314 20-21 -0.2528007866141877 2314 22-23 -0.20990241454534697 2314 24-25 -0.163716192473224 2314 26-27 -0.14472025685174117 2314 28-29 -0.1639511751420315 2314 30-31 -0.09142922383492902 2314 32-33 -0.237841119163825 2314 34-35 -0.018868549093710385 2314 36-37 0.12615689788333384 2314 38-39 0.29133620287860396 2314 40-41 0.3974816677985942 2314 42-43 0.6034329903170175 2314 44-45 0.5575849300992388 2314 46-47 0.6725947508115055 2314 48-49 0.7465130398027782 2314 50-51 0.896187789876528 2314 52-53 1.2149633345544757 2314 54-55 1.26084379936745 2314 56-57 1.662341666959243 2314 58-59 1.8908490198545174 2314 60-61 2.0366318506533077 2314 62-63 2.1771048152167687 2314 64-65 2.4927600411027804 2314 66-67 2.553269865234043 2314 68-69 3.01559902968528 2314 70-71 3.402229519263269 2314 72-73 3.5745515177297627 2314 74-75 3.861948062403396 2314 76-77 4.022844051133646 2314 78-79 4.0874628358742235 2314 80-81 4.394646880104585 2314 82-83 4.670282810017529 2314 84-85 4.962324384026303 2314 86-87 5.207582689413108 2314 88-89 5.375244434195949 2314 90-91 5.381809927584186 2314 92-93 5.621603291675346 2314 94-95 5.431912875562848 2314 96-97 5.241087671177951 2314 98-99 5.266269824777844 2314 100-101 4.711975097240527 2315 1 -0.413368668392156 2315 2 -0.35719698749071327 2315 3 -0.3332422092576657 2315 4 -0.2604810704896039 2315 5 -0.1667805385803618 2315 6 -0.12884409568011534 2315 7 -0.18160045116130874 2315 8 -0.1638664276384958 2315 9 -0.2952692777654917 2315 10-11 -0.19879903268100918 2315 12-13 -0.12977264293450474 2315 14-15 -0.2186422197154556 2315 16-17 -0.32437398409378915 2315 18-19 -0.3255763726495218 2315 20-21 -0.2724498652332912 2315 22-23 -0.15576960136569085 2315 24-25 -0.07356239535518938 2315 26-27 0.06917499213024314 2315 28-29 0.08138455609518758 2315 30-31 0.18182041459900233 2315 32-33 0.17892346571046858 2315 34-35 0.2639215138892581 2315 36-37 0.26653162355089677 2315 38-39 0.38399588933520334 2315 40-41 0.5584332732203237 2315 42-43 0.8111567329465572 2315 44-45 0.8034524208364218 2315 46-47 0.8342011070803039 2315 48-49 1.0869986761229669 2315 50-51 1.1975833570214505 2315 52-53 1.2855176294882895 2315 54-55 1.467189279117278 2315 56-57 1.6849083472641766 2315 58-59 1.9005473861875224 2315 60-61 2.1852038783750842 2315 62-63 2.2649728727588645 2315 64-65 2.4697301725354173 2315 66-67 2.548661631139055 2315 68-69 2.9464104610614257 2315 70-71 3.3451591598550117 2315 72-73 3.5915837773628816 2315 74-75 3.8602372955034348 2315 76-77 3.8819633039236976 2315 78-79 4.217359173638286 2315 80-81 4.516188638848558 2315 82-83 4.810121027484467 2315 84-85 4.8208849046966336 2315 86-87 5.193464612401343 2315 88-89 5.307673937835165 2315 90-91 5.2941357120030546 2315 92-93 5.385567130889658 2315 94-95 5.330437511252029 2315 96-97 5.376082878789244 2315 98-99 5.133352648575595 2315 100-101 4.900702232721718 2316 1 -0.8043120345789241 2316 2 -0.7090138866080196 2316 3 -0.7418825124183712 2316 4 -0.5541291630253014 2316 5 -0.3807114636873763 2316 6 -0.37685502657099335 2316 7 -0.4527181947460832 2316 8 -0.3832141506451663 2316 9 -0.5999743986786612 2316 10-11 -0.48333167210427774 2316 12-13 -0.46647842376214044 2316 14-15 -0.3796395250647251 2316 16-17 -0.27574759451429287 2316 18-19 -0.41283217833932895 2316 20-21 -0.5271079153244926 2316 22-23 -0.5080875893803167 2316 24-25 -0.428870765001129 2316 26-27 -0.3082401275254085 2316 28-29 -0.2688039963885842 2316 30-31 -0.2631433535587462 2316 32-33 -0.06948674035129443 2316 34-35 0.010382357233794437 2316 36-37 0.1525399563169927 2316 38-39 0.054594930920963236 2316 40-41 0.2724419767076043 2316 42-43 0.4172863423010327 2316 44-45 0.48242047382340303 2316 46-47 0.606786160479146 2316 48-49 0.8267455820047758 2316 50-51 0.8520684295332046 2316 52-53 1.0443257185936616 2316 54-55 1.339286530358173 2316 56-57 1.4092507113500616 2316 58-59 1.727193990070024 2316 60-61 1.9009258213386317 2316 62-63 1.9495096270334038 2316 64-65 2.4594802510829332 2316 66-67 2.750056200381426 2316 68-69 2.83993368516661 2316 70-71 3.115542767825822 2316 72-73 3.6312520972768 2316 74-75 3.617867460362838 2316 76-77 4.033386081048356 2316 78-79 4.372628952132381 2316 80-81 4.450797114596124 2316 82-83 4.672124971588634 2316 84-85 4.905602673970385 2316 86-87 5.143739807501056 2316 88-89 5.405727382446379 2316 90-91 5.736555002271967 2316 92-93 5.800528826239148 2316 94-95 5.924532045921957 2316 96-97 5.938675823472952 2316 98-99 5.587659724927626 2316 100-101 5.480167894287893 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5672.0 3 1759.0 4 1967.0 5 2122.0 6 2609.0 7 3298.0 8 4230.0 9 5497.0 10 6998.0 11 9005.0 12 11683.0 13 14675.0 14 17824.0 15 21741.0 16 25499.0 17 31341.0 18 32311.0 19 30832.0 20 33109.0 21 36392.0 22 40197.0 23 44891.0 24 50138.0 25 53918.0 26 58792.0 27 63789.0 28 68914.0 29 74384.0 30 80897.0 31 88877.0 32 98814.0 33 113510.0 34 135432.0 35 166868.0 36 203960.0 37 226698.0 38 128917.0 39 8912.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.32535340572804 18.64366246157002 14.967342333891178 23.063641798810767 2 15.241022655102658 19.59073656252212 40.93252522190994 24.235715560465284 3 19.468625891586118 23.527951115775934 29.789260329434995 27.214162663202956 4 12.683482316245959 16.691088596512657 37.40135044380771 33.22407864343367 5 13.724045004782331 37.02522019171216 34.47926442852883 14.771470374976687 6 32.15717250657994 36.20092971858111 17.220289048529473 14.42160872630948 7 28.389964229182507 32.72070095845849 20.41070382510937 18.478630987249637 8 26.028937936934383 35.13197954612417 19.446088223666532 19.392994293274917 9 25.715264306407875 17.298909111939047 20.446037161288004 36.53978942036507 10-11 24.916413845501527 26.557576546172484 27.117011144072794 21.408998464253195 12-13 26.021023453838886 23.899484259829347 27.50158909791585 22.577903188415913 14-15 22.57126192683875 25.42884276014716 26.218431888652926 25.781463424361164 16-17 21.929037183901443 28.529127966931245 27.05075050545109 22.491084343716217 18-19 21.88479039259404 27.068013823856745 29.350021997031334 21.697173786517876 20-21 22.43671546383066 26.11922569039432 29.775759734773704 21.668299111001314 22-23 22.16710029178093 26.030243918488782 29.677555401237417 22.125100388492875 24-25 22.042728601107395 26.19704081513085 29.72046879647333 22.039761787288427 26-27 21.66589926256912 26.495185297551277 29.683855271991362 22.155060167888237 28-29 21.703429078379255 27.047749049583203 29.38623178778067 21.86259008425687 30-31 21.89180302285828 26.453163496053346 29.37852389047231 22.276509590616072 32-33 21.656563178831984 26.486494032386005 29.396313485825452 22.460629302956562 34-35 22.438182671629352 26.591304031347583 29.181583019176937 21.788930277846124 36-37 22.363279302491616 26.20470064334459 29.246228875921844 22.18579117824195 38-39 21.927636312792213 26.639343751565512 29.11677480602583 22.316245129616444 40-41 22.446105381735894 26.44113586179052 29.090856980505723 22.021901775967862 42-43 22.09790631554602 26.447669851422372 28.882592372536664 22.57183146049494 44-45 22.20288848089212 26.666075190420873 28.935350234102476 22.195686094584527 46-47 22.615801030339433 26.88326059811528 28.342334887731884 22.1586034838134 48-49 22.354257982978186 26.912399395770244 28.314235362992697 22.419107258258876 50-51 22.37442717538776 27.166341189640956 28.330008782396927 22.129222852574358 52-53 22.677370800293065 27.056977955432394 28.07953588285428 22.18611536142026 54-55 22.291617448721134 27.09538861492976 28.287883364184886 22.325110572164224 56-57 22.514402659404922 27.123428118287595 28.091048582387547 22.271120639919932 58-59 22.545389571811913 26.829538159736206 28.06425712018692 22.56081514826496 60-61 22.214117427755877 27.072599741720936 28.365639813851672 22.34764301667151 62-63 22.215903412929563 27.07551079556938 28.46202978485119 22.246556006649868 64-65 22.010631932331645 27.11067980618193 28.291426892443834 22.58726136904259 66-67 22.047504876311034 27.081861468657415 28.110453460168504 22.760180194863047 68-69 22.465518895607826 26.788189014642246 28.291912811931102 22.454379277818827 70-71 22.471182005109352 26.846183562838803 27.912667455916257 22.769966976135585 72-73 22.42233605316342 26.838763896666894 28.026212851564768 22.712687198604918 74-75 22.331632327550114 27.315124031533237 27.77725164783897 22.575991993077682 76-77 22.586653588008307 26.870811365274367 27.791077404324927 22.7514576423924 78-79 22.633025940793626 27.029867350366686 27.7502345068478 22.586872201991888 80-81 22.857007568677588 27.078470514057006 27.39410206375433 22.670419853511074 82-83 22.81814898894679 27.05508462432016 27.55013770719943 22.576628679533627 84-85 22.79800854973916 27.14265564248394 27.520775008884176 22.538560798892732 86-87 22.936064904213357 27.082612226630477 27.288322320180598 22.693000548975572 88-89 22.905100341325515 27.142556616316128 27.09442608012052 22.85791696223784 90-91 22.961162870687463 27.048532703245648 27.138908412966643 22.851396013100242 92-93 23.097104618777923 26.722867094088986 27.288834059203598 22.891194227929493 94-95 22.872858027356234 26.62823321093252 27.40074916075849 23.09815960095275 96-97 23.05316852209743 26.354859919255986 27.416301238277583 23.175670320368997 98-99 22.957065291214043 26.562668624003212 26.88565407732061 23.59461200746214 100-101 23.827590387715563 25.439533545850846 27.181102739360362 23.55177332707323 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 524.0 1 358.0 2 303.5 3 397.5 4 323.5 5 312.5 6 571.0 7 1353.0 8 2302.5 9 2706.5 10 2326.5 11 1786.5 12 1716.0 13 1877.5 14 2069.0 15 2241.5 16 2438.5 17 2685.5 18 3006.0 19 3362.5 20 3771.0 21 4498.5 22 5526.0 23 6908.0 24 8767.0 25 10870.5 26 13493.5 27 16505.0 28 19217.5 29 22115.5 30 25187.0 31 28933.5 32 33573.5 33 36368.5 34 38145.5 35 41118.5 36 44806.5 37 48520.5 38 51346.0 39 53956.5 40 56835.5 41 59660.5 42 62081.5 43 64175.0 44 66175.5 45 69274.0 46 71093.5 47 73021.5 48 76094.5 49 74885.0 50 71655.5 51 69109.5 52 67202.0 53 66373.5 54 65940.5 55 62849.5 56 57866.0 57 52914.0 58 48872.5 59 44172.5 60 37039.0 61 31098.5 62 26229.0 63 20584.5 64 15621.5 65 12128.5 66 9310.0 67 7168.0 68 5462.5 69 4163.0 70 3133.5 71 2248.0 72 1644.5 73 1270.5 74 966.0 75 683.0 76 461.0 77 307.5 78 214.0 79 144.5 80 94.5 81 71.5 82 49.5 83 26.0 84 20.0 85 14.5 86 10.5 87 8.0 88 5.5 89 3.5 90 2.0 91 2.5 92 1.0 93 1.0 94 3.0 95 2.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3841566769151641 2 0.023872735890291073 3 0.04575192389830314 4 0.03797708715741503 5 0.058560469041945735 6 0.05656692115966674 7 0.032295475692919864 8 0.02960418605184321 9 0.03369095921051517 10-11 0.03032684715916935 12-13 0.030800314781210612 14-15 0.024570477649088724 16-17 0.029479589309200773 18-19 0.02594104181815554 20-21 0.03934765132648184 22-23 0.02571676768139915 24-25 0.047571036340882736 26-27 0.03954700611470974 28-29 0.0320213628591065 30-31 0.03929781262942487 32-33 0.036382248851591824 34-35 0.03511136207663896 36-37 0.03513628142516745 38-39 0.017069753742013975 40-41 0.01211080338484495 42-43 0.012409835567186799 44-45 0.009643787880524682 46-47 0.016845479605257584 48-49 0.014079431918595465 50-51 0.009319836349654343 52-53 0.004136611855728933 54-55 0.004535321432184734 56-57 0.007949272180587528 58-59 0.0032395153087033815 60-61 0.005781288858609112 62-63 0.005856046904194574 64-65 0.007899433483530553 66-67 0.0044605633865992715 68-69 0.0060055629953655 70-71 0.017343866575827333 72-73 0.01402959322153849 74-75 0.010416287684907797 76-77 0.00752564325560324 78-79 0.006728224102691639 80-81 0.008871288076141568 82-83 0.011138948792233934 84-85 0.004784514917469609 86-87 4.2362892498428833E-4 88-89 0.023424187616778298 90-91 0.020084994913960964 92-93 0.012982980583342013 94-95 0.024670155043202673 96-97 0.018938704881650538 98-99 0.017219269833184895 100-101 0.11193771358996608 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2006473.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.870996366125894 #Duplication Level Percentage of deduplicated Percentage of total 1 78.60110405006992 47.8452751900529 2 12.765841372861178 15.541389676359449 3 3.9541789930277584 7.220844453468121 4 1.6946565511035159 4.126217310562143 5 0.8411086029218547 2.5599559355986727 6 0.5075317297424218 1.8536377246106732 7 0.32304303979930404 1.376476619120799 8 0.21712340115231624 1.0573214210034831 9 0.1678626103762359 0.9196167911598236 >10 0.851999497264469 9.126021933180223 >50 0.04591666621216539 1.9224811171308465 >100 0.024778807914002388 2.8646842575744165 >500 0.0032913068169243094 1.4078251954325385 >1k 0.0013988053971928226 1.420246593200686 >5k 1.6456534084621443E-4 0.758005781545323 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8248 0.4110695733259306 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6886 0.3431892679343305 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4221 0.21036914027749187 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2376 0.11841674420737285 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 4.98386970569751E-5 4.98386970569751E-5 0.0 0.0 0.0 5 4.98386970569751E-5 4.98386970569751E-5 0.0 0.0 0.0 6 4.98386970569751E-5 4.98386970569751E-5 0.0 0.0 4.98386970569751E-5 7 4.98386970569751E-5 4.98386970569751E-5 0.0 0.0 4.98386970569751E-5 8 4.98386970569751E-5 4.98386970569751E-5 0.0 0.0 4.98386970569751E-5 9 4.98386970569751E-5 4.98386970569751E-5 0.0 4.98386970569751E-5 4.98386970569751E-5 10-11 4.98386970569751E-5 4.98386970569751E-5 0.0 4.98386970569751E-5 4.98386970569751E-5 12-13 4.98386970569751E-5 4.98386970569751E-5 0.0 4.98386970569751E-5 9.96773941139502E-5 14-15 4.98386970569751E-5 4.98386970569751E-5 0.0 1.495160911709253E-4 9.96773941139502E-5 16-17 4.98386970569751E-5 4.98386970569751E-5 0.0 1.993547882279004E-4 9.96773941139502E-5 18-19 4.98386970569751E-5 4.98386970569751E-5 0.0 1.993547882279004E-4 9.96773941139502E-5 20-21 4.98386970569751E-5 4.98386970569751E-5 0.0 2.7411283381336305E-4 9.96773941139502E-5 22-23 4.98386970569751E-5 4.98386970569751E-5 0.0 3.987095764558008E-4 9.96773941139502E-5 24-25 4.98386970569751E-5 7.475804558546266E-5 0.0 7.475804558546265E-4 9.96773941139502E-5 26-27 4.98386970569751E-5 9.96773941139502E-5 0.0 0.00132072547200984 9.96773941139502E-5 28-29 7.475804558546266E-5 1.495160911709253E-4 0.0 0.004012015113086496 9.96773941139502E-5 30-31 9.96773941139502E-5 1.7443543969941286E-4 0.0 0.01079007791283511 9.96773941139502E-5 32-33 9.96773941139502E-5 2.491934852848755E-4 0.0 0.026713541622538653 9.96773941139502E-5 34-35 9.96773941139502E-5 2.491934852848755E-4 0.0 0.05053643881577275 9.96773941139502E-5 36-37 1.495160911709253E-4 2.491934852848755E-4 0.0 0.08796530030556104 9.96773941139502E-5 38-39 1.993547882279004E-4 2.491934852848755E-4 0.0 0.14201536726385056 9.96773941139502E-5 40-41 1.993547882279004E-4 2.491934852848755E-4 0.0 0.20047615891168233 1.495160911709253E-4 42-43 1.993547882279004E-4 2.491934852848755E-4 0.0 0.26255025609614485 1.7443543969941286E-4 44-45 1.993547882279004E-4 2.491934852848755E-4 0.0 0.33416846376701803 2.2427413675638795E-4 46-47 1.993547882279004E-4 2.491934852848755E-4 0.0 0.4233298928019465 2.491934852848755E-4 48-49 1.993547882279004E-4 2.491934852848755E-4 0.0 0.5119680155177767 2.491934852848755E-4 50-51 1.993547882279004E-4 2.491934852848755E-4 0.0 0.6049171855290353 2.491934852848755E-4 52-53 1.993547882279004E-4 2.491934852848755E-4 0.0 0.6945770015345335 2.990321823418506E-4 54-55 1.993547882279004E-4 2.990321823418506E-4 0.0 0.7922359284176762 3.239515308703382E-4 56-57 1.993547882279004E-4 2.990321823418506E-4 0.0 0.9068400123001904 3.488708793988257E-4 58-59 1.993547882279004E-4 3.987095764558008E-4 0.0 1.0268017561163294 3.7379022792731324E-4 60-61 1.993547882279004E-4 3.987095764558008E-4 0.0 1.153267449898404 3.987095764558008E-4 62-63 1.993547882279004E-4 3.987095764558008E-4 0.0 1.278038627980541 3.987095764558008E-4 64-65 1.993547882279004E-4 4.485482735127759E-4 0.0 1.4081425466477744 3.987095764558008E-4 66-67 1.993547882279004E-4 4.98386970569751E-4 0.0 1.5596521857009789 3.987095764558008E-4 68-69 1.993547882279004E-4 4.98386970569751E-4 0.0 1.7243690794742816 3.987095764558008E-4 70-71 2.2427413675638795E-4 5.482256676267261E-4 0.0 1.8925746820415723 3.987095764558008E-4 72-73 2.491934852848755E-4 5.482256676267261E-4 0.0 2.0610294780941483 4.485482735127759E-4 74-75 2.491934852848755E-4 5.482256676267261E-4 0.0 2.23020693525405 4.485482735127759E-4 76-77 2.491934852848755E-4 5.482256676267261E-4 0.0 2.4136133404237183 4.485482735127759E-4 78-79 2.491934852848755E-4 5.482256676267261E-4 0.0 2.619521917314611 4.485482735127759E-4 80-81 2.990321823418506E-4 5.482256676267261E-4 0.0 2.8327567826728792 4.485482735127759E-4 82-83 2.990321823418506E-4 5.482256676267261E-4 0.0 3.0530687430132377 4.485482735127759E-4 84-85 2.990321823418506E-4 5.482256676267261E-4 0.0 3.2701411880448923 4.485482735127759E-4 86-87 2.990321823418506E-4 5.482256676267261E-4 0.0 3.4916243577660904 4.485482735127759E-4 88-89 2.990321823418506E-4 5.482256676267261E-4 0.0 3.7346378446159005 4.485482735127759E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6940 0.0 32.22758 1 GTATCAA 9885 0.0 30.296839 1 TATCAAC 11800 0.0 25.214624 2 ATCAACG 11775 0.0 25.1067 3 TCAACGC 11805 0.0 25.083158 4 CAACGCA 11935 0.0 24.730297 5 AACGCAG 12210 0.0 24.206196 6 GAGTACT 7645 0.0 22.865534 12-13 GTGTAGC 1180 0.0 22.631866 1 ACGCAGA 13375 0.0 21.98406 7 TAGCCCT 1390 0.0 21.883932 4 CGCAGAG 13510 0.0 21.729223 8 GTACTTT 8145 0.0 21.63954 14-15 AGAGTAC 10495 0.0 21.069475 10-11 AGTACTT 8030 0.0 20.349512 12-13 GCAGAGT 14325 0.0 20.061888 9 GTAAGGT 1125 0.0 19.434147 4 GTACATG 5745 0.0 18.926014 1 TAAGGTG 1245 0.0 18.324497 5 GTAGCCC 1065 0.0 18.297611 3 >>END_MODULE