FastQCFastQC Report
Thu 26 May 2016
SRR937987_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937987_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2006473
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77780.38764538570915236No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67650.33715878559043655No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43620.21739639656252538No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT24060.11991190511908209No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69000.031.0179141
GTATCAA97000.027.8990671
ATCAACG112250.023.7668443
TCAACGC113000.023.6511824
CAACGCA114650.023.3108025
TATCAAC115300.023.2235322
AACGCAG118700.022.5262836
GAGTACT74550.021.50096712-13
TAAGGTG12000.021.3996935
GTAAGGT11750.021.045564
GTACTTT78400.020.3239614-15
AGAGTAC102200.020.14512810-11
ACGCAGA133100.019.9107697
CGCAGAG133200.019.717548
GTACATG57950.019.3690241
TACATGG56950.019.208052
AGTACTT78800.019.19619212-13
GGTAAGG12800.018.5761223
GCAGAGT140900.017.9995699
ACATGGG58200.017.567523
Produced by FastQC (version 0.11.5)