##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937987_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2006473 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.41760890876677 33.0 31.0 34.0 31.0 34.0 2 32.65433524398285 34.0 31.0 34.0 31.0 34.0 3 32.72025439664526 34.0 31.0 34.0 31.0 34.0 4 36.19422638630074 37.0 35.0 37.0 35.0 37.0 5 36.1159726545037 37.0 35.0 37.0 35.0 37.0 6 36.169572179640596 37.0 37.0 37.0 35.0 37.0 7 36.144048786103774 37.0 36.0 37.0 35.0 37.0 8 36.13565644790635 37.0 36.0 37.0 35.0 37.0 9 37.8162402384682 39.0 38.0 39.0 35.0 39.0 10-11 37.784325530420794 39.0 38.0 39.0 35.0 39.0 12-13 37.698778154502946 39.0 37.0 39.0 35.0 39.0 14-15 38.915330034343846 40.0 38.0 41.0 35.5 41.0 16-17 38.81139541872729 40.0 38.0 41.0 35.0 41.0 18-19 39.079125908995536 40.0 38.5 41.0 36.0 41.0 20-21 39.04917310125778 40.0 39.0 41.0 35.5 41.0 22-23 38.9376826899739 40.0 38.0 41.0 35.0 41.0 24-25 38.81617544816203 40.0 38.0 41.0 35.0 41.0 26-27 38.64045516685248 40.0 38.0 41.0 34.0 41.0 28-29 38.51006517406414 40.0 38.0 41.0 34.0 41.0 30-31 38.25727657436706 40.0 38.0 41.0 34.0 41.0 32-33 38.01668076271149 40.0 37.0 41.0 33.5 41.0 34-35 37.9101657485548 40.0 37.0 41.0 33.0 41.0 36-37 37.84022635739429 40.0 37.0 41.0 33.0 41.0 38-39 37.62962148007972 40.0 37.0 41.0 33.0 41.0 40-41 37.39855108939916 40.0 37.0 41.0 32.0 41.0 42-43 37.12451326282486 40.0 36.0 41.0 31.0 41.0 44-45 36.82826980477684 39.0 36.0 41.0 31.0 41.0 46-47 36.4432863038775 39.0 35.5 41.0 30.0 41.0 48-49 36.0040760578388 39.0 35.0 40.0 29.5 41.0 50-51 35.595910585390385 39.0 35.0 40.0 28.0 41.0 52-53 35.02483287838909 38.0 34.0 40.0 26.5 41.0 54-55 34.604233149411925 38.0 34.0 40.0 25.5 41.0 56-57 34.185571896556795 38.0 33.0 40.0 24.0 41.0 58-59 33.627093661364995 37.0 33.0 40.0 22.5 41.0 60-61 33.097045910909344 36.5 32.0 39.0 21.0 40.5 62-63 32.493253335579396 36.0 31.0 39.0 18.5 40.0 64-65 31.87795101155111 35.0 31.0 39.0 14.0 40.0 66-67 31.264460822547825 35.0 30.0 38.0 8.5 40.0 68-69 30.569004666397205 34.5 29.0 38.0 7.0 40.0 70-71 29.883111061050908 34.0 28.5 37.0 2.0 39.0 72-73 29.158555086462663 34.0 27.5 36.0 2.0 39.0 74-75 28.467296096184697 33.5 26.0 36.0 2.0 38.5 76-77 27.19037559937263 31.5 25.5 34.5 2.0 36.5 78-79 27.819669140825717 33.0 26.0 35.0 2.0 37.0 80-81 27.493159888022415 33.0 26.0 35.0 2.0 37.0 82-83 26.882718332118102 33.0 25.0 35.0 2.0 36.0 84-85 26.162953849864913 32.0 23.5 35.0 2.0 36.0 86-87 25.380292433538852 31.5 20.5 34.5 2.0 35.0 88-89 24.49830972058931 31.0 16.5 34.0 2.0 35.0 90-91 23.76353631471742 31.0 7.0 34.0 2.0 35.0 92-93 22.751991180544167 30.0 2.0 34.0 2.0 35.0 94-95 21.769330810830745 29.0 2.0 34.0 2.0 35.0 96-97 20.65198510022313 29.0 2.0 34.0 2.0 35.0 98-99 19.17902707885927 26.5 2.0 34.0 2.0 35.0 100-101 15.511152654433925 12.5 2.0 30.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.19938782698010726 1101 2 0.21101287039846284 1101 3 0.1545478780529166 1101 4 0.05863333706545859 1101 5 0.023300641789205656 1101 6 0.051035502856038306 1101 7 0.17884144123294732 1101 8 0.1748782875927617 1101 9 -0.013922312639365941 1101 10-11 0.19397316759931016 1101 12-13 0.25617389255329925 1101 14-15 0.3349160186414437 1101 16-17 0.3662491130649457 1101 18-19 0.30020839284327394 1101 20-21 0.17353352830878777 1101 22-23 0.3471008304824892 1101 24-25 0.39887814868771443 1101 26-27 0.382327129980375 1101 28-29 0.48015363979767756 1101 30-31 0.5572605385069309 1101 32-33 0.4687342116334108 1101 34-35 0.42781774966639574 1101 36-37 0.46764758137032203 1101 38-39 0.5240774550863208 1101 40-41 0.7232260225583715 1101 42-43 0.8025502271213298 1101 44-45 0.6173995848173703 1101 46-47 0.6898527485441903 1101 48-49 0.7803354465185421 1101 50-51 0.932807886763726 1101 52-53 1.0573047670382394 1101 54-55 1.2077006435690194 1101 56-57 1.3347895575530515 1101 58-59 1.358513991553103 1101 60-61 1.2770008869550225 1101 62-63 1.4597396445598605 1101 64-65 1.504872451006527 1101 66-67 1.7813160968135122 1101 68-69 2.105446460428549 1101 70-71 2.3246445603699684 1101 72-73 2.498817767375602 1101 74-75 2.4175283861238306 1101 76-77 2.3759600389176967 1101 78-79 2.5810656414278377 1101 80-81 2.889794325553634 1101 82-83 2.769491493548955 1101 84-85 3.213737477773318 1101 86-87 3.219545462537333 1101 88-89 3.5682471736968715 1101 90-91 3.9217128760731264 1101 92-93 4.072216159676927 1101 94-95 4.468964946040572 1101 96-97 4.645076932172778 1101 98-99 4.6196744816612245 1101 100-101 4.502459092778304 1102 1 0.2535874267401752 1102 2 0.19985203894217562 1102 3 0.225283473750423 1102 4 0.09990682668464501 1102 5 -0.008318666691032206 1102 6 0.03995164061737455 1102 7 0.03420271586678325 1102 8 0.15835880612346642 1102 9 0.20556027727749182 1102 10-11 0.26299037390570845 1102 12-13 0.269923071990668 1102 14-15 0.45572510372220876 1102 16-17 0.3811099226395669 1102 18-19 0.3759021627011876 1102 20-21 0.30269436330389254 1102 22-23 0.4221601203721548 1102 24-25 0.4747736309780137 1102 26-27 0.42063435570896957 1102 28-29 0.41130172562337464 1102 30-31 0.4628905271296375 1102 32-33 0.47257618468768925 1102 34-35 0.5398001464229552 1102 36-37 0.5851337195386179 1102 38-39 0.6547919771428639 1102 40-41 0.7221477162586254 1102 42-43 0.7656566103761335 1102 44-45 0.8662127548697995 1102 46-47 0.8274421552206306 1102 48-49 1.070206385949966 1102 50-51 1.0270189463850912 1102 52-53 1.082008347080027 1102 54-55 1.1362872174243819 1102 56-57 1.3870578492433836 1102 58-59 1.467670939750164 1102 60-61 1.4243503443072925 1102 62-63 1.7008203572535265 1102 64-65 1.7441004468047794 1102 66-67 1.9150279948390292 1102 68-69 1.9958450960975576 1102 70-71 2.142471545364806 1102 72-73 2.395994854966574 1102 74-75 2.4967102475757024 1102 76-77 2.5135323413235575 1102 78-79 2.720742211467172 1102 80-81 2.861740225335584 1102 82-83 2.7824817447045262 1102 84-85 3.1282910297700077 1102 86-87 3.3268654710540773 1102 88-89 3.4006515681968423 1102 90-91 3.708277677961437 1102 92-93 3.7124076272410385 1102 94-95 4.071106046836043 1102 96-97 4.20913493216452 1102 98-99 4.677688186310579 1102 100-101 3.969199691180057 1103 1 0.2761985662394082 1103 2 0.19227297802126486 1103 3 0.2192470234012518 1103 4 0.10368585353980819 1103 5 0.07051798805262166 1103 6 0.11695101607867997 1103 7 0.13927567334137336 1103 8 0.10189880498794679 1103 9 0.26448550296306195 1103 10-11 0.2607102399137773 1103 12-13 0.2844972501545229 1103 14-15 0.36911776823138354 1103 16-17 0.4135899090132682 1103 18-19 0.379710997084878 1103 20-21 0.3686799989139544 1103 22-23 0.3240240844895652 1103 24-25 0.36939237849404805 1103 26-27 0.48082256896054787 1103 28-29 0.47699042147302606 1103 30-31 0.4911489158198137 1103 32-33 0.633918091233987 1103 34-35 0.7461165036590884 1103 36-37 0.8135774826681299 1103 38-39 0.8426032529050858 1103 40-41 0.9358904063171067 1103 42-43 0.893361714560541 1103 44-45 1.0370158548527613 1103 46-47 1.2471673581957106 1103 48-49 1.3263748410683363 1103 50-51 1.5005526998626308 1103 52-53 1.6698485787271053 1103 54-55 1.896417802787461 1103 56-57 1.902848174571261 1103 58-59 2.125961690899885 1103 60-61 2.2023313810415246 1103 62-63 2.365390561080247 1103 64-65 2.391020619404557 1103 66-67 2.675811507017535 1103 68-69 2.638725071116017 1103 70-71 2.7162727149554513 1103 72-73 2.860575846279808 1103 74-75 2.9020236524471024 1103 76-77 2.818321985336503 1103 78-79 3.0436906018606287 1103 80-81 3.207460148119889 1103 82-83 3.3020590455618475 1103 84-85 3.50176573595294 1103 86-87 3.7126078204550907 1103 88-89 4.023041280341282 1103 90-91 4.281779636741884 1103 92-93 4.258815394231327 1103 94-95 4.301380559979741 1103 96-97 4.526407685244635 1103 98-99 4.844865555299393 1103 100-101 4.455415554462858 1104 1 0.25275445830238397 1104 2 0.21372478015490515 1104 3 0.1921710909573875 1104 4 0.12036698093059783 1104 5 0.012679184200067084 1104 6 0.09707079452947198 1104 7 0.13340816631564678 1104 8 0.21453174815457032 1104 9 0.18809058877245377 1104 10-11 0.22887809345119337 1104 12-13 0.3302868602606637 1104 14-15 0.41574555464921303 1104 16-17 0.4285652166447065 1104 18-19 0.3478211441477157 1104 20-21 0.32742505361914453 1104 22-23 0.29429610980947984 1104 24-25 0.343844917306086 1104 26-27 0.4733516950416927 1104 28-29 0.5023901377154232 1104 30-31 0.45962030814198584 1104 32-33 0.4822085880826492 1104 34-35 0.4638267980576032 1104 36-37 0.6207202618555954 1104 38-39 0.5494164367199872 1104 40-41 0.6672401648503055 1104 42-43 0.6184636217390675 1104 44-45 0.641349024479787 1104 46-47 0.732290820038493 1104 48-49 0.9323224277644684 1104 50-51 1.0334658536647083 1104 52-53 1.2204471981372222 1104 54-55 1.4178603282301552 1104 56-57 1.3747696773952 1104 58-59 1.571841066633155 1104 60-61 1.6216061620843334 1104 62-63 1.5700841574350477 1104 64-65 1.6653777992905106 1104 66-67 1.8485050215191876 1104 68-69 2.00653318003528 1104 70-71 2.1385403202049673 1104 72-73 2.353646840249958 1104 74-75 2.426192297231694 1104 76-77 2.529789066725826 1104 78-79 2.5892778982509093 1104 80-81 2.752651138815917 1104 82-83 2.834984340915316 1104 84-85 3.031170936356748 1104 86-87 3.0751106171045635 1104 88-89 3.2676307867667553 1104 90-91 3.5628038925602787 1104 92-93 3.9455494533293063 1104 94-95 4.144174071752953 1104 96-97 4.447852529860057 1104 98-99 4.649601715295027 1104 100-101 3.987865146561303 1105 1 0.17822773554822646 1105 2 0.2534661718492117 1105 3 0.1778900164259838 1105 4 0.06955564850915863 1105 5 0.012448869473040247 1105 6 -0.0193372950606161 1105 7 0.10745364839950611 1105 8 0.20061683500274086 1105 9 0.2100149893295793 1105 10-11 0.16786147643882288 1105 12-13 0.2139857303912649 1105 14-15 0.4009249971470439 1105 16-17 0.38067614870888633 1105 18-19 0.3750037470320322 1105 20-21 0.32609703548127555 1105 22-23 0.3034977687000975 1105 24-25 0.3825682398341499 1105 26-27 0.333823520133663 1105 28-29 0.4669872508959827 1105 30-31 0.5153869204699859 1105 32-33 0.5258637686806154 1105 34-35 0.5141993614058649 1105 36-37 0.5628593469674286 1105 38-39 0.5825150051773349 1105 40-41 0.6824054137151236 1105 42-43 0.6905657940363383 1105 44-45 0.8218240349415424 1105 46-47 0.8133526255810395 1105 48-49 0.9209394151541588 1105 50-51 1.0624819959945242 1105 52-53 1.0321535882388346 1105 54-55 1.339723129356905 1105 56-57 1.4169297640872216 1105 58-59 1.3345169646263741 1105 60-61 1.3134025076532296 1105 62-63 1.541206984669337 1105 64-65 1.6200768443041582 1105 66-67 1.7586354343056314 1105 68-69 2.07637514509614 1105 70-71 2.2394427635709633 1105 72-73 2.181944478704242 1105 74-75 2.1022757133221823 1105 76-77 2.1725689732883104 1105 78-79 2.340122645825968 1105 80-81 2.582926751375016 1105 82-83 2.6733404871175424 1105 84-85 2.9773401388275964 1105 86-87 3.0039394628139995 1105 88-89 3.2508630939113523 1105 90-91 3.7403866965601047 1105 92-93 3.800773225556629 1105 94-95 3.8266606276187183 1105 96-97 4.156401344090543 1105 98-99 4.233868408818733 1105 100-101 3.672729018846711 1106 1 0.1989616575792894 1106 2 0.12531852816369593 1106 3 0.0971939392492942 1106 4 0.1267388578259414 1106 5 0.005878582254368325 1106 6 0.044534276201673606 1106 7 0.05526614268620733 1106 8 0.1651906080920611 1106 9 0.17633093596894867 1106 10-11 0.2158299055421864 1106 12-13 0.17517571034141355 1106 14-15 0.1284930843669443 1106 16-17 0.05654121748170837 1106 18-19 0.05098765230410862 1106 20-21 0.0454242685409767 1106 22-23 0.10509109191708887 1106 24-25 0.1129320318763618 1106 26-27 -0.12391067918257193 1106 28-29 0.0864072678377994 1106 30-31 0.21298383315868108 1106 32-33 0.3477065266624706 1106 34-35 0.14602865125113595 1106 36-37 0.22590264306532504 1106 38-39 0.2559443066015419 1106 40-41 0.2729401627263357 1106 42-43 0.23247813231205328 1106 44-45 0.30365842866209647 1106 46-47 0.5231139013365649 1106 48-49 0.6450955236549234 1106 50-51 0.8445987025780681 1106 52-53 0.7781032442224145 1106 54-55 0.8962333127307645 1106 56-57 0.9341724830663267 1106 58-59 1.0824754145108813 1106 60-61 1.0847401343359877 1106 62-63 1.110323740006315 1106 64-65 1.2699052931526609 1106 66-67 1.412868451351052 1106 68-69 1.4935144915563434 1106 70-71 1.4558672627817657 1106 72-73 2.0114978972342037 1106 74-75 2.465902070038883 1106 76-77 2.2593740844766153 1106 78-79 2.2814021797431216 1106 80-81 2.1696259761953876 1106 82-83 2.7360149330042773 1106 84-85 2.886598949487535 1106 86-87 2.8622010371843167 1106 88-89 2.9601562605079366 1106 90-91 3.2320940418745963 1106 92-93 3.101369769547464 1106 94-95 3.3061971348007084 1106 96-97 3.715992516062194 1106 98-99 3.8819101204299713 1106 100-101 3.397540251638274 1107 1 0.2038943465221692 1107 2 0.17023880939476754 1107 3 0.16062836581738082 1107 4 0.08658369907814745 1107 5 0.02222371616334584 1107 6 0.10743326947797271 1107 7 0.14930054291129835 1107 8 0.1081088987894745 1107 9 0.1428449223126691 1107 10-11 0.16298256170438208 1107 12-13 0.17126244897592358 1107 14-15 0.3458178195924475 1107 16-17 0.266683196932604 1107 18-19 0.20847089760659543 1107 20-21 0.17808975211050182 1107 22-23 0.18934779438063032 1107 24-25 0.30593118313276335 1107 26-27 0.24762858631518725 1107 28-29 0.29044904878089994 1107 30-31 0.27807171202449155 1107 32-33 0.3244510298980643 1107 34-35 0.43041893925501284 1107 36-37 0.3232649911156713 1107 38-39 0.4058890096698775 1107 40-41 0.43174367585458384 1107 42-43 0.5961035846440694 1107 44-45 0.6257581844826987 1107 46-47 0.7268575698573727 1107 48-49 0.9586743301608678 1107 50-51 0.8381544082939527 1107 52-53 1.044597044653095 1107 54-55 1.0742695585250388 1107 56-57 1.018761590622951 1107 58-59 1.1558531568528991 1107 60-61 1.3335891865723042 1107 62-63 1.221705945071811 1107 64-65 1.4188095254138062 1107 66-67 1.7469538853053166 1107 68-69 2.0360841660799274 1107 70-71 2.141915133791443 1107 72-73 2.1786477788075835 1107 74-75 1.9993830177324305 1107 76-77 2.057181769620019 1107 78-79 2.0613496184313327 1107 80-81 2.289507697656287 1107 82-83 2.4649286425126213 1107 84-85 2.571748976025386 1107 86-87 2.8841908226446904 1107 88-89 3.110040172023492 1107 90-91 3.3196451788714754 1107 92-93 3.6566222766144847 1107 94-95 3.6166611536055626 1107 96-97 3.706445124760215 1107 98-99 3.950630294574392 1107 100-101 3.8490800315260874 1108 1 0.14163337968812328 1108 2 0.1740533724034492 1108 3 0.09808941239259639 1108 4 0.1218472948790037 1108 5 0.04606771141764199 1108 6 0.08781638705328731 1108 7 0.11604542791649664 1108 8 0.06116820490282748 1108 9 0.1640386310663402 1108 10-11 0.18156142447895718 1108 12-13 0.14729551941257313 1108 14-15 0.31751399464647534 1108 16-17 0.2621272902020877 1108 18-19 0.2699925973118624 1108 20-21 0.34131938038650134 1108 22-23 0.3047107067054924 1108 24-25 0.2708450668186302 1108 26-27 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2316 84-85 2.4911114223814756 2316 86-87 2.603032939442194 2316 88-89 2.7730620742047805 2316 90-91 3.0487668369636864 2316 92-93 3.017911092212014 2316 94-95 3.6669008686530944 2316 96-97 3.949314436318982 2316 98-99 4.203665287289596 2316 100-101 4.29244757701265 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 1.0 4 9.0 5 37.0 6 78.0 7 227.0 8 555.0 9 1045.0 10 1775.0 11 2983.0 12 4733.0 13 6921.0 14 9601.0 15 13276.0 16 17241.0 17 23147.0 18 24522.0 19 21156.0 20 21056.0 21 22633.0 22 24531.0 23 27023.0 24 30399.0 25 33845.0 26 38270.0 27 44366.0 28 51689.0 29 61549.0 30 72949.0 31 86753.0 32 103164.0 33 125776.0 34 159617.0 35 210302.0 36 279815.0 37 324060.0 38 155835.0 39 5528.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.453000862707846 19.14897434453392 15.396569004417204 23.001455788341037 2 15.552388685601093 19.922320302145312 40.16809001354311 24.35720099871049 3 19.488827286202294 23.429904017432165 30.031259874621586 27.050008821743955 4 13.06371524134999 17.00307459519159 37.02646922937756 32.90674093408086 5 13.762169229003558 37.04620190316512 34.490451739744415 14.701177128086904 6 32.298731305495416 36.399710536703225 16.958572060811214 14.34298609699014 7 28.465122630606043 32.681326885534965 20.258084708839842 18.59546577501915 8 26.18098306274122 35.10439260310676 19.32254951995152 19.392074814200505 9 25.634882702134544 17.129360823694114 20.33403888315467 36.90171759101668 10-11 24.87389563677159 26.57997391442596 27.043972184026398 21.502158264776053 12-13 25.942985527340763 23.758854467515885 27.536228994858142 22.76193101028521 14-15 22.435836415441422 25.40044147117853 26.152557248465342 26.011164864914704 16-17 21.930521865980754 28.540254466419434 26.989523407491657 22.53970026010816 18-19 21.875948492703365 27.0909451560026 29.294812339862037 21.738294011432 20-21 22.33541144087162 26.15355402240648 29.777749314344128 21.733285222377773 22-23 22.118842662828413 26.039712569550076 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48-49 22.386047557081508 26.87509375904884 28.327642584774377 22.411216099095277 50-51 22.296038870196607 27.184891099954996 28.36736402632879 22.151706003519607 52-53 22.72093868195585 27.00078695302653 28.085426517077476 22.192847847940143 54-55 22.30002596596117 27.10171031456691 28.230506964210335 22.367756755261595 56-57 22.45165206682477 27.19440570175056 28.035509101425067 22.318433129999605 58-59 22.521509135682365 26.933778824833425 27.96496638629077 22.579745653193438 60-61 22.113828593756306 27.24031173108235 28.27159896993381 22.37426070522753 62-63 22.194442686245967 27.17878585956552 28.335317744121152 22.291453710067366 64-65 21.926534770216193 27.310160665007704 28.157244079536582 22.606060485239524 66-67 21.9547155179417 27.14522163539867 28.031952617306704 22.86811022935293 68-69 22.345424555889725 26.901269427605044 28.228934058093984 22.524371958411237 70-71 22.310292737554903 26.990869052312195 27.80254207258209 22.896296137550816 72-73 22.24834049433059 26.991095311120866 27.88941380164478 22.87115039290376 74-75 22.14806777863445 27.443180147452768 27.620257038096202 22.78849503581658 76-77 22.187564946052102 27.05707976135238 27.919687930014508 22.835667362581006 78-79 22.29683628934952 27.17953344002137 27.93758002225796 22.586050248371148 80-81 22.46041808707024 27.221760383399317 27.625927498614484 22.691894030915957 82-83 22.374991683016134 27.22979271958021 27.8218622916798 22.573353305723852 84-85 22.373915680710414 27.359149076785094 27.67643605877335 22.59049918373114 86-87 22.446768320459647 27.333486840728106 27.506905340761495 22.712839498050755 88-89 22.381263042164036 27.479811589789644 27.1366970799009 23.002228288145417 90-91 22.360578134333323 27.2908033439667 27.425022533921744 22.923595987778228 92-93 22.427413675638793 27.023413721490396 27.58103398351236 22.96813861935845 94-95 22.150172043267986 27.009920895980606 27.64329629319522 23.196610767556187 96-97 22.299264579819702 26.812559557272664 27.65687734491187 23.231298517995764 98-99 22.163268581236828 27.011577030939364 27.22523552522262 23.59991886260119 100-101 22.508625832493138 26.296366808823247 27.560400762930776 23.634606595752846 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 519.0 1 353.5 2 273.5 3 365.5 4 314.5 5 306.0 6 571.5 7 1375.0 8 2354.0 9 2690.5 10 2225.0 11 1731.0 12 1715.5 13 1841.5 14 2017.5 15 2178.0 16 2329.5 17 2563.0 18 2838.5 19 3141.0 20 3590.5 21 4396.5 22 5362.5 23 6496.5 24 8270.5 25 10464.0 26 12950.5 27 15907.5 28 18801.0 29 21666.5 30 24642.0 31 28518.0 32 33701.5 33 36657.0 34 38380.5 35 41474.0 36 44884.0 37 48431.0 38 51592.0 39 54392.5 40 57546.0 41 60570.0 42 62778.5 43 65216.0 44 67717.5 45 70812.5 46 72515.0 47 75047.5 48 78198.0 49 76684.0 50 73195.5 51 70093.5 52 68559.5 53 68253.0 54 67810.0 55 63909.0 56 58080.5 57 52872.0 58 48628.5 59 43508.5 60 35831.0 61 29733.5 62 24840.0 63 18827.5 64 13549.5 65 10155.5 66 7565.0 67 5806.0 68 4433.0 69 3341.5 70 2483.0 71 1725.5 72 1197.0 73 915.0 74 702.0 75 492.0 76 325.5 77 190.0 78 132.5 79 86.0 80 42.5 81 28.5 82 19.5 83 14.0 84 7.5 85 4.5 86 4.0 87 1.5 88 1.5 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.014253867358294878 3 0.003339192702817331 4 0.0014951609117092532 5 0.12175593691019015 6 4.98386970569751E-5 7 0.0 8 4.485482735127759E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.491934852848755E-4 24-25 6.479030617406763E-4 26-27 0.005880966252723062 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.011288464883404861 36-37 5.482256676267261E-4 38-39 0.007052175633561977 40-41 0.01056580377607872 42-43 0.005856046904194574 44-45 0.003962176416029521 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 7.475804558546264E-5 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 9.96773941139502E-5 68-69 8.721771984970642E-4 70-71 0.0 72-73 9.96773941139502E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 4.98386970569751E-5 82-83 0.0018191124425795911 84-85 0.0043608859924853215 86-87 0.0027411283381336307 88-89 0.0 90-91 0.002666370292548168 92-93 0.0 94-95 4.98386970569751E-5 96-97 4.98386970569751E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2006473.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.328897152079584 #Duplication Level Percentage of deduplicated Percentage of total 1 77.42157653209081 45.93336751424275 2 13.351576654987099 15.84268636363673 3 4.205353631739336 7.484969793167625 4 1.8141061992202108 4.305156804659436 5 0.9036473056659104 2.680619902980331 6 0.5392846578983502 1.919709840248739 7 0.36782790488385364 1.5275976756963345 8 0.23944643767247997 1.136487445928192 9 0.1804155101660936 0.9633467922555724 >10 0.8932642516408714 9.441494841130687 >50 0.05178040906255233 2.0921056836932186 >100 0.02650576412339246 2.9919256605291324 >500 0.0037007838283372473 1.5695291699586273 >1k 0.001345739573940809 1.3852954976384213 >5k 1.6821744674260113E-4 0.7257070142341977 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7778 0.38764538570915236 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6765 0.33715878559043655 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4362 0.21739639656252538 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2406 0.11991190511908209 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 4.98386970569751E-5 0.0 2 0.0 0.0 0.0 4.98386970569751E-5 0.0 3 0.0 0.0 0.0 4.98386970569751E-5 0.0 4 0.0 0.0 0.0 4.98386970569751E-5 0.0 5 0.0 0.0 0.0 4.98386970569751E-5 0.0 6 0.0 0.0 0.0 4.98386970569751E-5 0.0 7 0.0 4.98386970569751E-5 0.0 4.98386970569751E-5 0.0 8 0.0 4.98386970569751E-5 0.0 4.98386970569751E-5 0.0 9 0.0 4.98386970569751E-5 0.0 4.98386970569751E-5 0.0 10-11 0.0 4.98386970569751E-5 0.0 4.98386970569751E-5 0.0 12-13 0.0 4.98386970569751E-5 0.0 4.98386970569751E-5 0.0 14-15 0.0 7.475804558546266E-5 0.0 9.96773941139502E-5 2.491934852848755E-5 16-17 0.0 9.96773941139502E-5 0.0 1.495160911709253E-4 4.98386970569751E-5 18-19 4.98386970569751E-5 9.96773941139502E-5 0.0 1.495160911709253E-4 4.98386970569751E-5 20-21 9.96773941139502E-5 1.495160911709253E-4 0.0 2.4919348528487553E-4 9.96773941139502E-5 22-23 9.96773941139502E-5 1.495160911709253E-4 0.0 3.7379022792731324E-4 1.495160911709253E-4 24-25 9.96773941139502E-5 1.495160911709253E-4 0.0 6.479030617406764E-4 1.495160911709253E-4 26-27 9.96773941139502E-5 1.495160911709253E-4 0.0 0.0012459674264243773 1.495160911709253E-4 28-29 9.96773941139502E-5 1.495160911709253E-4 0.0 0.00396217641602952 1.495160911709253E-4 30-31 9.96773941139502E-5 1.495160911709253E-4 0.0 0.010715319867249646 1.495160911709253E-4 32-33 9.96773941139502E-5 1.993547882279004E-4 0.0 0.02663878357695319 2.990321823418506E-4 34-35 1.2459674264243776E-4 1.993547882279004E-4 0.0 0.05056135816430124 2.990321823418506E-4 36-37 1.495160911709253E-4 1.993547882279004E-4 0.0 0.08828925183643138 2.990321823418506E-4 38-39 1.495160911709253E-4 1.993547882279004E-4 0.0 0.14221472205207844 2.990321823418506E-4 40-41 1.495160911709253E-4 1.993547882279004E-4 0.0 0.2007253523969672 2.990321823418506E-4 42-43 1.495160911709253E-4 1.993547882279004E-4 0.0 0.26324799785494246 2.990321823418506E-4 44-45 2.990321823418506E-4 1.993547882279004E-4 0.0 0.3349160442228727 2.990321823418506E-4 46-47 3.239515308703382E-4 1.993547882279004E-4 0.0 0.42427682804602906 3.239515308703382E-4 48-49 3.488708793988257E-4 1.993547882279004E-4 0.0 0.5137871279603563 4.485482735127759E-4 50-51 3.488708793988257E-4 1.993547882279004E-4 0.0 0.607508797775998 4.485482735127759E-4 52-53 3.488708793988257E-4 2.491934852848755E-4 0.0 0.6966951461594549 4.98386970569751E-4 54-55 3.488708793988257E-4 2.491934852848755E-4 0.0 0.7944288310881831 4.98386970569751E-4 56-57 3.987095764558008E-4 2.491934852848755E-4 0.0 0.9094565438956816 5.233063190982386E-4 58-59 3.987095764558008E-4 3.488708793988257E-4 0.0 1.0294681264088776 5.482256676267261E-4 60-61 3.987095764558008E-4 3.488708793988257E-4 0.0 1.1562079330247652 5.482256676267261E-4 62-63 3.987095764558008E-4 3.488708793988257E-4 0.0 1.280754836970146 5.482256676267261E-4 64-65 3.987095764558008E-4 3.488708793988257E-4 0.0 1.4118306102299907 5.482256676267261E-4 66-67 3.987095764558008E-4 3.488708793988257E-4 0.0 1.5658321841360436 5.482256676267261E-4 68-69 3.987095764558008E-4 3.488708793988257E-4 0.0 1.732418029048983 5.482256676267261E-4 70-71 4.236289249842884E-4 3.488708793988257E-4 0.0 1.9025424214529674 5.482256676267261E-4 72-73 4.485482735127759E-4 3.488708793988257E-4 0.0 2.0724923784172526 5.482256676267261E-4 74-75 4.485482735127759E-4 3.488708793988257E-4 0.0 2.245382818507899 5.482256676267261E-4 76-77 4.485482735127759E-4 3.488708793988257E-4 0.0 2.433399303155338 5.482256676267261E-4 78-79 4.485482735127759E-4 3.488708793988257E-4 0.0 2.64523868499601 5.482256676267261E-4 80-81 4.98386970569751E-4 3.488708793988257E-4 0.0 2.8667716934142646 6.229837132121888E-4 82-83 4.98386970569751E-4 3.488708793988257E-4 0.0 3.0968769577263187 6.977417587976514E-4 84-85 5.233063190982386E-4 3.488708793988257E-4 0.0 3.324938835459037 6.977417587976514E-4 86-87 5.482256676267261E-4 3.488708793988257E-4 0.0 3.5596292599003325 6.977417587976514E-4 88-89 5.482256676267261E-4 3.488708793988257E-4 0.0 3.817494678473122 6.977417587976514E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6900 0.0 31.017914 1 GTATCAA 9700 0.0 27.899067 1 ATCAACG 11225 0.0 23.766844 3 TCAACGC 11300 0.0 23.651182 4 CAACGCA 11465 0.0 23.310802 5 TATCAAC 11530 0.0 23.223532 2 AACGCAG 11870 0.0 22.526283 6 GAGTACT 7455 0.0 21.500967 12-13 TAAGGTG 1200 0.0 21.399693 5 GTAAGGT 1175 0.0 21.04556 4 GTACTTT 7840 0.0 20.32396 14-15 AGAGTAC 10220 0.0 20.145128 10-11 ACGCAGA 13310 0.0 19.910769 7 CGCAGAG 13320 0.0 19.71754 8 GTACATG 5795 0.0 19.369024 1 TACATGG 5695 0.0 19.20805 2 AGTACTT 7880 0.0 19.196192 12-13 GGTAAGG 1280 0.0 18.576122 3 GCAGAGT 14090 0.0 17.999569 9 ACATGGG 5820 0.0 17.56752 3 >>END_MODULE