FastQCFastQC Report
Thu 26 May 2016
SRR937986_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937986_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1971927
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82330.4175103845122056No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69110.3504693632167925No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41470.2103019026566399No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT22830.11577507686643573No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64850.032.829161
GTATCAA93650.030.4809321
ATCAACG112250.025.0657583
TATCAAC112900.025.0471042
TCAACGC113500.024.831584
CAACGCA113900.024.7443735
AACGCAG117400.024.0036326
GAGTACT73150.023.37801712-13
GTACTTT76700.022.08202214-15
ACGCAGA128800.021.833887
CGCAGAG129250.021.7211078
GTTACGT1100.002868418821.6034343
AGAGTAC100700.021.15638710-11
TACATGG58550.021.1047842
GTGTAGC12900.021.0921081
GTACATG60500.020.987521
GCAGAGT137100.019.9918219
AGTACTT77150.019.85698512-13
ACATGGG60650.019.4341873
ATAGGAC4950.019.2030533
Produced by FastQC (version 0.11.5)