FastQCFastQC Report
Thu 26 May 2016
SRR937986_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937986_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1971927
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79300.40214470413965625No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68490.34732523059930714No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42680.21643803244237744No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT23920.12130266485524059No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66600.030.8831061
GTATCAA93500.029.5169731
TCAACGC110400.024.822644
ATCAACG110300.024.8033453
TATCAAC111800.024.6005082
CAACGCA111600.024.5557295
AACGCAG115300.023.8089226
GAGTACT73800.022.5887412-13
TAAGGTG12400.021.4490245
ACGCAGA128000.021.3353187
CGCAGAG127850.021.286058
GTACATG56850.021.2231831
GTACTTT79000.021.01170514-15
GTATACG1604.9904003E-520.7819041
AGAGTAC100250.020.63217510-11
GTGTAGC12000.020.5839821
TACATGG56850.020.5547372
GTAAGGT11550.019.737884
GCAGAGT136650.019.7067349
AGTACTT78800.019.55823712-13
Produced by FastQC (version 0.11.5)