##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937986_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1971927 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.42707108326018 33.0 31.0 34.0 31.0 34.0 2 32.66793141936796 34.0 31.0 34.0 31.0 34.0 3 32.63480189682478 34.0 31.0 34.0 31.0 34.0 4 36.17527525106153 37.0 35.0 37.0 35.0 37.0 5 36.13250338374595 37.0 35.0 37.0 35.0 37.0 6 36.19466085712098 37.0 37.0 37.0 35.0 37.0 7 36.156938872483614 37.0 36.0 37.0 35.0 37.0 8 36.14852273943204 37.0 36.0 37.0 35.0 37.0 9 37.84714292161931 39.0 38.0 39.0 35.0 39.0 10-11 37.806829056045174 39.0 38.0 39.0 35.0 39.0 12-13 37.74699316962545 39.0 38.0 39.0 35.0 39.0 14-15 38.999726156191386 40.0 38.0 41.0 36.0 41.0 16-17 38.92757921566062 40.0 38.0 41.0 35.5 41.0 18-19 38.91790340108939 40.0 38.0 41.0 35.0 41.0 20-21 38.92212186353754 40.0 38.0 41.0 35.0 41.0 22-23 38.95156362279131 40.0 38.0 41.0 35.0 41.0 24-25 38.892719913059665 40.0 38.0 41.0 35.0 41.0 26-27 38.712370944766214 40.0 38.0 41.0 35.0 41.0 28-29 38.63331350501312 40.0 38.0 41.0 34.5 41.0 30-31 38.41409037961344 40.0 38.0 41.0 34.0 41.0 32-33 38.203856177231714 40.0 38.0 41.0 34.0 41.0 34-35 37.9823360601077 40.0 37.5 41.0 33.0 41.0 36-37 37.89117269554096 40.0 37.0 41.0 33.0 41.0 38-39 37.84096064408064 40.0 37.0 41.0 33.0 41.0 40-41 37.716344976259265 40.0 37.0 41.0 33.0 41.0 42-43 37.52364337016533 40.0 37.0 41.0 33.0 41.0 44-45 37.258043781539584 40.0 37.0 41.0 32.0 41.0 46-47 36.89541093559751 40.0 36.0 41.0 31.0 41.0 48-49 36.51158790360901 39.0 36.0 41.0 30.0 41.0 50-51 36.20312516639815 39.0 35.0 41.0 30.0 41.0 52-53 35.71115690388133 39.0 35.0 40.0 28.0 41.0 54-55 35.3806142418051 38.0 35.0 40.0 27.5 41.0 56-57 35.08022482576688 38.0 34.0 40.0 27.0 41.0 58-59 34.67875027828109 38.0 34.0 40.0 26.0 41.0 60-61 34.231661719728976 37.0 33.5 40.0 25.5 41.0 62-63 33.7802078880202 37.0 33.0 39.5 24.5 41.0 64-65 33.310523411870726 36.0 32.5 39.0 22.5 40.5 66-67 32.761630881873415 35.5 32.0 39.0 21.5 40.0 68-69 32.19197972338733 35.0 31.0 38.0 19.5 40.0 70-71 31.55793748957238 35.0 30.5 37.5 18.0 39.5 72-73 31.06032550900718 34.5 30.0 37.0 15.5 39.0 74-75 30.480971151568998 34.0 29.5 36.0 10.0 39.0 76-77 29.29989117244198 32.5 28.5 35.0 10.0 37.5 78-79 29.99903393989737 34.0 29.5 35.5 7.5 37.5 80-81 29.772370123234786 34.0 29.0 35.0 6.5 37.0 82-83 29.26703980421182 34.0 29.0 35.0 2.0 37.0 84-85 28.69823629373704 34.0 29.0 35.0 2.0 36.0 86-87 28.096907745570704 33.0 27.0 35.0 2.0 36.0 88-89 27.39775204660213 33.0 26.0 35.0 2.0 35.0 90-91 26.792533648557985 32.0 25.0 35.0 2.0 35.0 92-93 26.078998614046057 32.0 24.5 34.0 2.0 35.0 94-95 25.238474091586554 31.5 20.5 34.0 2.0 35.0 96-97 24.316448834059273 31.0 12.5 34.0 2.0 35.0 98-99 23.177799178164303 31.0 2.0 34.0 2.0 35.0 100-101 19.692037788417117 26.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.09486076279972622 1101 2 0.09401733391337785 1101 3 0.23946080940546466 1101 4 0.13599069341264425 1101 5 0.06743573594246044 1101 6 0.05661110697016625 1101 7 0.09508244812575128 1101 8 0.1138555482293171 1101 9 0.21099222425782216 1101 10-11 0.1548089934680874 1101 12-13 0.22202915261141953 1101 14-15 0.34433113634128887 1101 16-17 0.3651167670174331 1101 18-19 0.36475418688930716 1101 20-21 0.35036480827040606 1101 22-23 0.23080202766428215 1101 24-25 0.21642603168248087 1101 26-27 0.3243411394447264 1101 28-29 0.30205033365588463 1101 30-31 0.41113788023732667 1101 32-33 0.45090646845164173 1101 34-35 0.40002710171230405 1101 36-37 0.42007859335303976 1101 38-39 0.4771515997891953 1101 40-41 0.6102698477767277 1101 42-43 0.6491856624090957 1101 44-45 0.6353734812144509 1101 46-47 0.7228016113177347 1101 48-49 0.7699960527100913 1101 50-51 0.8345312396095821 1101 52-53 0.8063656419094585 1101 54-55 0.9028262456648264 1101 56-57 0.7837172844286187 1101 58-59 0.9110551234188122 1101 60-61 1.0402990672182213 1101 62-63 0.9720837796008297 1101 64-65 0.9044976054732246 1101 66-67 1.0012552144608193 1101 68-69 1.0871942744382679 1101 70-71 1.1396624394800874 1101 72-73 1.2665915828685854 1101 74-75 1.2000867032949003 1101 76-77 1.435553694317214 1101 78-79 1.4651780393988325 1101 80-81 1.6282304310723426 1101 82-83 1.8070630446655223 1101 84-85 1.9519303390852052 1101 86-87 2.161391629758697 1101 88-89 2.2179396628572405 1101 90-91 2.398321860974221 1101 92-93 2.7041594727243634 1101 94-95 2.7352367705490863 1101 96-97 2.8350873723311736 1101 98-99 2.986047691035953 1101 100-101 3.2602334828664503 1102 1 0.029000483196817584 1102 2 0.08812308178774231 1102 3 0.23304272862814202 1102 4 0.10935843417325941 1102 5 -0.06186194225019648 1102 6 0.006116467816490001 1102 7 0.0927664425926551 1102 8 0.12073888434326108 1102 9 0.03329549911685348 1102 10-11 0.18099820526281007 1102 12-13 0.16316047259577005 1102 14-15 0.1916041321276154 1102 16-17 0.21350820776275015 1102 18-19 0.31975336380931196 1102 20-21 0.17386772569341957 1102 22-23 0.22353407862139818 1102 24-25 0.26026038523711037 1102 26-27 0.2634751371456474 1102 28-29 0.10332009763873629 1102 30-31 0.16955224230313348 1102 32-33 0.1979533046181956 1102 34-35 0.13995339371887638 1102 36-37 0.2863591850712126 1102 38-39 0.33004291464352775 1102 40-41 0.252227967578861 1102 42-43 0.21930957967025222 1102 44-45 0.3434421358020572 1102 46-47 0.4057282950160044 1102 48-49 0.5581653812239509 1102 50-51 0.5256329259381047 1102 52-53 0.37423393134380234 1102 54-55 0.4968378043173942 1102 56-57 0.6172789889176329 1102 58-59 0.6781856666821469 1102 60-61 0.8198633927936925 1102 62-63 0.4992983020076238 1102 64-65 0.6951336755868738 1102 66-67 0.6869992468532544 1102 68-69 0.817276501934252 1102 70-71 1.047069271127338 1102 72-73 1.019167767735965 1102 74-75 0.9099895909593272 1102 76-77 0.9151836442134318 1102 78-79 1.1225187331678264 1102 80-81 1.2460295568031547 1102 82-83 1.3326859552151866 1102 84-85 1.5910483471039214 1102 86-87 1.6743252284393257 1102 88-89 1.7136602020759604 1102 90-91 1.9779121697832842 1102 92-93 2.1558224690742165 1102 94-95 2.100334375622257 1102 96-97 2.4795913493482225 1102 98-99 2.754262118226407 1102 100-101 3.165456584737104 1103 1 0.27868596252204014 1103 2 0.24744914667508766 1103 3 0.42154973952795416 1103 4 0.1416495806573792 1103 5 0.004479951903682888 1103 6 0.14499172330872057 1103 7 0.22005069141155076 1103 8 0.2252708397152574 1103 9 0.15797178036442006 1103 10-11 0.3106927076343027 1103 12-13 0.19821568606618456 1103 14-15 0.34429150679265064 1103 16-17 0.40188608836849227 1103 18-19 0.32980570176419377 1103 20-21 0.3644309981086806 1103 22-23 0.4416885477440786 1103 24-25 0.5118906775252299 1103 26-27 0.4644765309669765 1103 28-29 0.48876866734659075 1103 30-31 0.44830650467446986 1103 32-33 0.4386811065312557 1103 34-35 0.4226251441541322 1103 36-37 0.419336954657048 1103 38-39 0.4598127876707636 1103 40-41 0.42026863058345043 1103 42-43 0.4483022419261289 1103 44-45 0.503840178594011 1103 46-47 0.4972098749278331 1103 48-49 0.5985138651307054 1103 50-51 0.5797408410000173 1103 52-53 0.5743165890202775 1103 54-55 0.5988012634804321 1103 56-57 0.4571943951797621 1103 58-59 0.5364329310131097 1103 60-61 0.6242033137366576 1103 62-63 0.6683650418302634 1103 64-65 0.7514725263379702 1103 66-67 0.7610375208580891 1103 68-69 0.8834255043625419 1103 70-71 0.9431708221911315 1103 72-73 0.8849554911063677 1103 74-75 0.7782200737101661 1103 76-77 0.48207170771624774 1103 78-79 1.0022916424537058 1103 80-81 1.1341752172036976 1103 82-83 1.1900557407981758 1103 84-85 1.203932692797327 1103 86-87 1.3099302547690463 1103 88-89 1.513311603862995 1103 90-91 1.5774750199800032 1103 92-93 1.816220690469624 1103 94-95 1.7985890269918947 1103 96-97 1.9275077257842845 1103 98-99 2.2716320181156 1103 100-101 2.4391110042080264 1104 1 0.1209527020800607 1104 2 0.12091033818933994 1104 3 0.2166494820087621 1104 4 0.07927786198428777 1104 5 0.10140275645693464 1104 6 0.0957183995342703 1104 7 0.09522230061791248 1104 8 0.07908443888155148 1104 9 0.12696279929347298 1104 10-11 0.12699044287915484 1104 12-13 0.07784929676279972 1104 14-15 0.20337935319681577 1104 16-17 0.11944457168356593 1104 18-19 0.2611485628536272 1104 20-21 0.23178186427717407 1104 22-23 0.1919017419872162 1104 24-25 0.1504658006915136 1104 26-27 0.06225567924057174 1104 28-29 0.16039424832116822 1104 30-31 0.1468903761003375 1104 32-33 0.15433867539704949 1104 34-35 0.24298686426040916 1104 36-37 0.28573339154085176 1104 38-39 0.371875431435015 1104 40-41 0.4449474449446029 1104 42-43 0.5079141084311942 1104 44-45 0.5724695941467246 1104 46-47 0.588666314237031 1104 48-49 0.5529202902053285 1104 50-51 0.7227674275380735 1104 52-53 0.7624936631327657 1104 54-55 0.7281430493575414 1104 56-57 0.8492556900648509 1104 58-59 0.6999101145274764 1104 60-61 0.8606877714710208 1104 62-63 0.736165350645777 1104 64-65 0.9555412452464651 1104 66-67 1.1045318701568334 1104 68-69 1.1539675397822933 1104 70-71 1.1804034863377346 1104 72-73 1.0291454859248574 1104 74-75 1.22752702218882 1104 76-77 1.2321085100684819 1104 78-79 1.361176670608316 1104 80-81 1.4978197460395606 1104 82-83 1.5115082372298048 1104 84-85 1.7856603411237764 1104 86-87 1.9803063391414355 1104 88-89 2.177323475296653 1104 90-91 2.1828746870403073 1104 92-93 2.3100299511736004 1104 94-95 2.4233815881826963 1104 96-97 2.8744799135819896 1104 98-99 2.9984528590769095 1104 100-101 3.1655631731473584 1105 1 0.1816889071246166 1105 2 0.18492987121625504 1105 3 0.2322652249159276 1105 4 0.16719140521092868 1105 5 0.08629040121586229 1105 6 0.1102310729424616 1105 7 0.12876997590652195 1105 8 0.0727103712504018 1105 9 0.055540273313283706 1105 10-11 0.09053062680801105 1105 12-13 0.1129734684406074 1105 14-15 0.1785864031422406 1105 16-17 0.14361802149320368 1105 18-19 0.2279276582060774 1105 20-21 0.2093615278817751 1105 22-23 0.20811320304485292 1105 24-25 0.1525229518624016 1105 26-27 0.16749401196652514 1105 28-29 0.14519061180576642 1105 30-31 0.11391731818230966 1105 32-33 0.048124460745931685 1105 34-35 0.21042124957836705 1105 36-37 0.23957770882103802 1105 38-39 0.15944711871166817 1105 40-41 0.19751258864791055 1105 42-43 0.23694057440496152 1105 44-45 0.1547702133438591 1105 46-47 0.24667163536408054 1105 48-49 0.37926914881634843 1105 50-51 0.5298348364858754 1105 52-53 0.5115979291975066 1105 54-55 0.6532837775699321 1105 56-57 0.806896399041733 1105 58-59 0.8081687732499816 1105 60-61 0.6981798237048906 1105 62-63 0.7408888942897676 1105 64-65 0.825254071792699 1105 66-67 1.0424150212656969 1105 68-69 1.0336522917991644 1105 70-71 1.1691494261862907 1105 72-73 1.0728364576589087 1105 74-75 1.0877623343556202 1105 76-77 1.1709240591748937 1105 78-79 1.426702869266677 1105 80-81 1.4590980662589317 1105 82-83 1.4635442992786913 1105 84-85 1.6809921957704113 1105 86-87 1.69321379112084 1105 88-89 1.8196411243386876 1105 90-91 1.7468770829697426 1105 92-93 1.6821239016382918 1105 94-95 1.8010332042809374 1105 96-97 2.177233203962995 1105 98-99 2.5376087952392403 1105 100-101 2.728861665712305 1106 1 0.019826563311760026 1106 2 0.024077326812040667 1106 3 0.08489256953745183 1106 4 0.024440603799277483 1106 5 0.06908234106747813 1106 6 0.0333308046302605 1106 7 5.275245199669598E-4 1106 8 -0.06720179486264044 1106 9 -0.021065556567656074 1106 10-11 0.0011260559623877953 1106 12-13 -0.02643068770299095 1106 14-15 0.04739542042287326 1106 16-17 -0.03209951666974575 1106 18-19 -0.08075060352896202 1106 20-21 -0.009488130196409372 1106 22-23 0.08921191456568778 1106 24-25 0.09369954442181694 1106 26-27 0.1209592620111124 1106 28-29 0.059393948498183136 1106 30-31 0.11534804013705724 1106 32-33 0.12110475825315348 1106 34-35 0.02760090263866033 1106 36-37 -0.033754463346646446 1106 38-39 0.08110783440440628 1106 40-41 0.2652603834699363 1106 42-43 -0.007175840744935158 1106 44-45 -0.0074933546693429776 1106 46-47 -0.2991239082480561 1106 48-49 -0.0365888489193793 1106 50-51 0.024722597385284928 1106 52-53 -0.05202669083177369 1106 54-55 0.01760468228384582 1106 56-57 0.2293616366452511 1106 58-59 0.11270623535659041 1106 60-61 0.08239294736488034 1106 62-63 0.10633675173179569 1106 64-65 0.2200960081193486 1106 66-67 0.20028378466575703 1106 68-69 0.041770083759750776 1106 70-71 0.30100807915119887 1106 72-73 0.3254138306875163 1106 74-75 0.360453791908796 1106 76-77 0.5371899163432268 1106 78-79 0.7299744534698505 1106 80-81 1.0369967296743354 1106 82-83 1.0053079025415634 1106 84-85 1.0082511813263402 1106 86-87 1.1504735646106 1106 88-89 1.284804081495821 1106 90-91 1.2475654883160807 1106 92-93 1.3464226197943638 1106 94-95 1.5897871076636605 1106 96-97 1.7257533398193736 1106 98-99 2.1000670658488936 1106 100-101 2.38650441651372 1107 1 0.08451667878069458 1107 2 0.049865684064570814 1107 3 0.09249390439735805 1107 4 0.0211891016381216 1107 5 0.05078703881579116 1107 6 0.02729020855851161 1107 7 -0.012800217312452844 1107 8 0.04013771574790326 1107 9 0.009951297813685756 1107 10-11 0.11010906845007895 1107 12-13 0.03984546143919232 1107 14-15 0.11413397781563361 1107 16-17 0.1419219706694932 1107 18-19 0.22872166528910753 1107 20-21 0.15481435225249385 1107 22-23 0.15402096037161783 1107 24-25 0.17983151271889852 1107 26-27 0.11744549183067221 1107 28-29 0.18437198188743764 1107 30-31 0.25971726558104535 1107 32-33 0.11349854192419429 1107 34-35 0.22504168154361537 1107 36-37 0.36777743039708355 1107 38-39 0.3682205342118081 1107 40-41 0.4050252741694038 1107 42-43 0.30580221039511457 1107 44-45 0.3591477952272655 1107 46-47 0.3982740468601804 1107 48-49 0.48411900847072076 1107 50-51 0.4128821504202236 1107 52-53 0.24884110123539926 1107 54-55 0.29052184819167337 1107 56-57 0.3552684804648649 1107 58-59 0.35880860420039085 1107 60-61 0.5351691719479703 1107 62-63 0.5776752061699426 1107 64-65 0.5940176472279006 1107 66-67 0.8743647371735008 1107 68-69 0.849883750985569 1107 70-71 1.0125642914862247 1107 72-73 0.8686132786377456 1107 74-75 0.965792122091365 1107 76-77 1.0278203802602945 1107 78-79 1.2325336745648947 1107 80-81 1.2887897820125573 1107 82-83 1.3172761552419026 1107 84-85 1.5794230001617144 1107 86-87 1.607716777149765 1107 88-89 1.6107888069353749 1107 90-91 1.8019137678201247 1107 92-93 1.7760287296185417 1107 94-95 1.8942330165488208 1107 96-97 1.9703795080436741 1107 98-99 2.2190411141843107 1107 100-101 2.419756318067801 1108 1 0.0900055539645237 1108 2 0.044971109973168666 1108 3 0.15947763227818257 1108 4 0.11651081346678183 1108 5 0.014706967741176413 1108 6 0.03461287782434397 1108 7 0.0508708642034037 1108 8 0.030671379152671818 1108 9 0.0406074010443902 1108 10-11 0.031428757584883726 1108 12-13 0.011568287690231216 1108 14-15 0.1724945176031909 1108 16-17 0.199425021961396 1108 18-19 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-1.0051273791557378 2316 58-59 -1.0370371153402331 2316 60-61 -1.1060261059664498 2316 62-63 -1.1237079500138876 2316 64-65 -1.0835829216465385 2316 66-67 -1.215093442000562 2316 68-69 -1.318827620087383 2316 70-71 -1.6340074914860345 2316 72-73 -1.5354517141978619 2316 74-75 -1.5022733840144156 2316 76-77 -1.3503052212281865 2316 78-79 -1.5757374604037722 2316 80-81 -1.8410873539398764 2316 82-83 -2.0023869042760474 2316 84-85 -2.1428753808986194 2316 86-87 -2.3788569305856484 2316 88-89 -2.3688306246179067 2316 90-91 -2.6374577970123134 2316 92-93 -2.983107479453512 2316 94-95 -3.086352004516641 2316 96-97 -3.3698988340936715 2316 98-99 -3.700010298377972 2316 100-101 -4.035893396425667 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 3.0 4 14.0 5 27.0 6 80.0 7 192.0 8 437.0 9 851.0 10 1565.0 11 2458.0 12 3893.0 13 5715.0 14 7983.0 15 10837.0 16 14030.0 17 18914.0 18 19220.0 19 15271.0 20 13932.0 21 14395.0 22 14889.0 23 16697.0 24 18971.0 25 21755.0 26 25410.0 27 30545.0 28 36513.0 29 44675.0 30 53994.0 31 66248.0 32 82682.0 33 107343.0 34 145793.0 35 210665.0 36 312184.0 37 406064.0 38 234932.0 39 12744.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.495893610666116 19.070381408642408 15.385153710051133 23.048571270640345 2 15.543859079668351 19.913182575248868 40.196700958146266 24.34625738693651 3 19.754469493034833 23.41567045945557 29.970605716975047 26.859254330534554 4 13.046223313540512 17.041249498586915 37.04117850204394 32.871348685828636 5 13.766988518804007 37.03436244878088 34.53431173678445 14.664337295630656 6 32.282211544556716 36.39342267074052 16.934264741306084 14.39010104339668 7 28.43994732056511 32.721900962865256 20.244157111292658 18.59399460527697 8 26.163848864587784 35.179243450695694 19.334691395776822 19.322216288939703 9 25.57878663865346 17.13628344254123 20.35237612751385 36.93255379129146 10-11 24.917732755827167 26.55785432219347 27.048693993236057 21.475718928743305 12-13 25.934986437124703 23.77501804072869 27.53968579972788 22.75030972241873 14-15 22.444644249001104 25.416179199331417 26.110373254182328 26.028803297485148 16-17 21.925538299728718 28.54994848945942 27.031661677045264 22.492851533766597 18-19 21.85793388903342 27.11200769602526 29.312215918743444 21.717842496197882 20-21 22.334878522379377 26.16073008787851 29.763120034362327 21.741271355379787 22-23 22.128227870504332 26.099647705011392 29.633297784350027 22.138826640134244 24-25 22.085027141500937 26.25557333885089 29.633630307603674 22.025769212044498 26-27 21.724262903515832 26.539125530030027 29.607599614196218 22.129011952257923 28-29 21.69843183213915 27.050299733406902 29.349202477383706 21.902065957070242 30-31 21.919475111749968 26.497743701647806 29.316994641529128 22.265786545073098 32-33 21.713895943028195 26.409784707527567 29.40067778903341 22.475641560410832 34-35 22.422348245655414 26.608282346592166 29.160079347085677 21.80929006066674 36-37 22.344535147208287 26.235264463698492 29.26143798142171 22.158762407671507 38-39 21.86510736355802 26.636372997125505 29.18009778445522 22.31842185486125 40-41 22.390533834996457 26.45012471832174 29.07378734099523 22.085554105686573 42-43 22.073410144711787 26.467412902455163 28.93454582066451 22.52463113216854 44-45 22.158904609813526 26.685085464829523 29.015211357610816 22.14079856774614 46-47 22.593391323751096 26.880143416616903 28.37546957151243 22.150995688119572 48-49 22.32025404655965 26.883872688676323 28.39914560515071 22.396727659613312 50-51 22.348722848259595 27.17684275330679 28.37823103999286 22.096203358440754 52-53 22.67038789975491 27.028713537570106 28.150737831572876 22.150160731102115 54-55 22.238931765729664 27.056579680687975 28.326276784079734 22.378211769502624 56-57 22.41840088400838 27.17980939456684 28.122415282107298 22.27937443931748 58-59 22.406762522142046 26.94972988350989 28.065389844553067 22.578117749795 60-61 22.072394160635763 27.177856989635014 28.401026001469624 22.348722848259595 62-63 22.130104207711543 27.180468648180184 28.442127928670786 22.247299215437486 64-65 21.873172789864938 27.271623138179052 28.260909252725884 22.594294819230125 66-67 21.897730819679364 27.151661816495416 28.11021827165202 22.8403890921732 68-69 22.230567143924628 26.911730139769553 28.323143203505 22.53455951280082 70-71 22.229042961529508 26.991161437517718 27.88678282715334 22.893012773799438 72-73 22.187053577541157 26.992048894304908 28.013942706804055 22.80695482134988 74-75 22.135200745260857 27.494273368131783 27.67726695765107 22.693258928956293 76-77 22.16684491870135 27.043673523411364 27.966831429358187 22.822650128529098 78-79 22.24661460591594 27.236175578507726 27.94152623296907 22.57568358260727 80-81 22.418308210661266 27.293622877960814 27.68225347427183 22.605815437106095 82-83 22.351738402053034 27.280230375450422 27.850286384556306 22.517744837940242 84-85 22.30546324240523 27.436733302871264 27.719513289797256 22.538290164926256 86-87 22.408572377612625 27.387564879824943 27.52385371580418 22.680009026758253 88-89 22.262245134959425 27.55364552219505 27.30082163326616 22.883287709579367 90-91 22.28219375448022 27.377505975952165 27.457151529151513 22.883148740416097 92-93 22.336597582506577 27.127515665581512 27.643916318042557 22.891970433869353 94-95 22.11230703812443 27.100175488463172 27.70364686883296 23.083870604579435 96-97 22.221593957570327 26.91153605467153 27.74772531570721 23.119144672050922 98-99 22.119378423847795 27.08760105937226 27.340755024601627 23.452265492178316 100-101 22.378484684895305 26.570997289108433 27.618865533337676 23.431652492658582 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 529.0 1 376.0 2 314.0 3 400.5 4 324.0 5 291.5 6 601.5 7 1439.5 8 2409.0 9 2774.0 10 2319.5 11 1810.5 12 1787.0 13 1937.0 14 2053.0 15 2126.5 16 2309.5 17 2523.5 18 2747.5 19 3123.0 20 3559.5 21 4194.5 22 5143.5 23 6327.0 24 7992.0 25 10198.0 26 12753.5 27 15646.0 28 18185.0 29 21043.0 30 24305.0 31 28137.0 32 33186.0 33 36043.0 34 37375.5 35 40520.0 36 44547.0 37 47742.0 38 50681.0 39 53674.5 40 56696.5 41 60090.5 42 62013.0 43 64008.0 44 66961.5 45 70089.5 46 71752.0 47 74570.5 48 77698.0 49 75575.5 50 72046.0 51 69526.0 52 67815.0 53 67378.0 54 66970.0 55 62958.0 56 57120.5 57 51723.0 58 47654.0 59 42584.0 60 34532.0 61 28468.5 62 23754.5 63 17811.0 64 12584.0 65 9413.0 66 7042.5 67 5297.5 68 4086.5 69 3089.5 70 2251.5 71 1528.5 72 1052.0 73 826.5 74 619.5 75 422.5 76 284.5 77 166.0 78 101.5 79 63.0 80 42.0 81 31.5 82 19.0 83 10.5 84 8.5 85 5.0 86 1.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.010294498731443913 3 0.0058318588872711815 4 0.0 5 3.549827148773763E-4 6 1.0142363282210752E-4 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.535590820552688E-5 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0019777608400310966 26-27 0.02325136782446815 28-29 4.056945312884301E-4 30-31 1.2677954102763438E-4 32-33 0.003778030322623505 34-35 0.017039170314114064 36-37 0.008266026075001761 38-39 0.022718893752152084 40-41 0.024569875051155543 42-43 0.01965082885928333 44-45 0.010624125538115762 46-47 0.002510234912347161 48-49 2.5355908205526876E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.7749135743868816E-4 68-69 1.2677954102763438E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.5213544923316127E-4 82-83 7.860331543713332E-4 84-85 0.0038540980472400854 86-87 6.085417969326451E-4 88-89 5.071181641105376E-5 90-91 0.003321623974924021 92-93 4.310504394939569E-4 94-95 1.2677954102763438E-4 96-97 2.5355908205526876E-4 98-99 2.2820317384974188E-4 100-101 0.0033723357913350745 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1971927.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.49909757490924 #Duplication Level Percentage of deduplicated Percentage of total 1 77.44574021847662 46.07951654020213 2 13.34385438146661 15.878945877365238 3 4.219811863090932 7.5322499336942075 4 1.82158040268003 4.335295604784062 5 0.9147128933757767 2.7212295847996444 6 0.5439871347967101 1.9420046167658855 7 0.3297320412349619 1.3733131226509097 8 0.2576477039346955 1.2263844701089417 9 0.17126658386788787 0.9171186466389154 >10 0.8688035366168486 9.235581435362262 >50 0.05172131934363068 2.1003623571219623 >100 0.026024092491511365 3.0000665161829634 >500 0.003497182893000947 1.4696483492563748 >1k 0.0014500514434394132 1.4382334612050665 >5k 1.7059428746346036E-4 0.7500494838614516 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7930 0.40214470413965625 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6849 0.34732523059930714 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4268 0.21643803244237744 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2392 0.12130266485524059 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 5.0711816411053754E-5 0.0 0.0 0.0 0.0 8 5.0711816411053754E-5 0.0 0.0 0.0 0.0 9 5.0711816411053754E-5 0.0 0.0 0.0 5.0711816411053754E-5 10-11 5.0711816411053754E-5 0.0 0.0 0.0 5.0711816411053754E-5 12-13 5.0711816411053754E-5 0.0 0.0 0.0 5.0711816411053754E-5 14-15 1.0142363282210751E-4 0.0 0.0 0.0 7.606772461658063E-5 16-17 1.0142363282210751E-4 0.0 0.0 5.0711816411053754E-5 1.0142363282210751E-4 18-19 1.0142363282210751E-4 0.0 0.0 1.0142363282210751E-4 1.0142363282210751E-4 20-21 1.0142363282210751E-4 5.0711816411053754E-5 0.0 1.5213544923316127E-4 1.5213544923316127E-4 22-23 1.0142363282210751E-4 5.0711816411053754E-5 0.0 2.5355908205526876E-4 1.5213544923316127E-4 24-25 1.0142363282210751E-4 5.0711816411053754E-5 0.0 5.578299805215914E-4 1.5213544923316127E-4 26-27 1.0142363282210751E-4 5.0711816411053754E-5 0.0 0.0011917276856597634 2.5355908205526876E-4 28-29 1.0142363282210751E-4 5.0711816411053754E-5 0.0 0.003778030322623505 2.5355908205526876E-4 30-31 1.0142363282210751E-4 5.0711816411053754E-5 0.0 0.011131243702226299 2.5355908205526876E-4 32-33 1.0142363282210751E-4 5.0711816411053754E-5 0.0 0.027435092678380082 3.0427089846632254E-4 34-35 1.0142363282210751E-4 5.0711816411053754E-5 0.0 0.05192890000491905 3.549827148773763E-4 36-37 1.0142363282210751E-4 5.0711816411053754E-5 0.0 0.09059666001834754 3.549827148773763E-4 38-39 1.0142363282210751E-4 1.5213544923316127E-4 0.0 0.14670928487717852 3.549827148773763E-4 40-41 1.0142363282210751E-4 1.5213544923316127E-4 0.0 0.2063970927929888 3.549827148773763E-4 42-43 1.2677954102763438E-4 1.5213544923316127E-4 0.0 0.26542564709545535 3.549827148773763E-4 44-45 1.5213544923316127E-4 1.5213544923316127E-4 0.0 0.3412905244463918 3.549827148773763E-4 46-47 1.5213544923316127E-4 1.5213544923316127E-4 0.0 0.4296558645426529 3.549827148773763E-4 48-49 1.5213544923316127E-4 1.5213544923316127E-4 0.0 0.5191368645999573 3.549827148773763E-4 50-51 1.5213544923316127E-4 1.5213544923316127E-4 0.0 0.6140186731050388 3.549827148773763E-4 52-53 1.5213544923316127E-4 1.7749135743868813E-4 0.0 0.7056295694516075 3.549827148773763E-4 54-55 1.5213544923316127E-4 2.0284726564421502E-4 0.0 0.8057600509552332 3.549827148773763E-4 56-57 1.5213544923316127E-4 2.0284726564421502E-4 0.0 0.9203434001360091 3.549827148773763E-4 58-59 1.5213544923316127E-4 2.5355908205526876E-4 0.0 1.0434970462902533 3.549827148773763E-4 60-61 1.5213544923316127E-4 2.5355908205526876E-4 0.0 1.171011908655848 3.803386230829032E-4 62-63 1.5213544923316127E-4 2.5355908205526876E-4 0.0 1.2977407378670711 4.0569453128843003E-4 64-65 1.5213544923316127E-4 2.5355908205526876E-4 0.0 1.4261937688362702 4.0569453128843003E-4 66-67 1.5213544923316127E-4 2.5355908205526876E-4 0.0 1.5794195221222693 4.0569453128843003E-4 68-69 1.5213544923316127E-4 2.5355908205526876E-4 0.0 1.7476306171577345 4.0569453128843003E-4 70-71 1.7749135743868813E-4 2.5355908205526876E-4 0.0 1.9223581806020202 4.0569453128843003E-4 72-73 2.0284726564421502E-4 2.5355908205526876E-4 0.0 2.0971364558627172 4.3105043949395695E-4 74-75 2.0284726564421502E-4 2.5355908205526876E-4 0.0 2.27006374982441 4.564063476994838E-4 76-77 2.0284726564421502E-4 2.5355908205526876E-4 0.0 2.4588131305063525 4.564063476994838E-4 78-79 2.0284726564421502E-4 2.5355908205526876E-4 0.0 2.6724873689543274 4.8176225590501067E-4 80-81 3.549827148773763E-4 2.5355908205526876E-4 0.0 2.8955940052547584 5.831858887271181E-4 82-83 4.0569453128843003E-4 2.5355908205526876E-4 0.0 3.123391484573212 7.099654297547526E-4 84-85 4.0569453128843003E-4 2.5355908205526876E-4 0.0 3.355119129663522 7.099654297547526E-4 86-87 4.0569453128843003E-4 2.5355908205526876E-4 0.0 3.599245813866335 7.353213379602795E-4 88-89 4.0569453128843003E-4 2.5355908205526876E-4 0.0 3.8569886207755157 7.860331543713332E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6660 0.0 30.883106 1 GTATCAA 9350 0.0 29.516973 1 TCAACGC 11040 0.0 24.82264 4 ATCAACG 11030 0.0 24.803345 3 TATCAAC 11180 0.0 24.600508 2 CAACGCA 11160 0.0 24.555729 5 AACGCAG 11530 0.0 23.808922 6 GAGTACT 7380 0.0 22.58874 12-13 TAAGGTG 1240 0.0 21.449024 5 ACGCAGA 12800 0.0 21.335318 7 CGCAGAG 12785 0.0 21.28605 8 GTACATG 5685 0.0 21.223183 1 GTACTTT 7900 0.0 21.011705 14-15 GTATACG 160 4.9904003E-5 20.781904 1 AGAGTAC 10025 0.0 20.632175 10-11 GTGTAGC 1200 0.0 20.583982 1 TACATGG 5685 0.0 20.554737 2 GTAAGGT 1155 0.0 19.73788 4 GCAGAGT 13665 0.0 19.706734 9 AGTACTT 7880 0.0 19.558237 12-13 >>END_MODULE