FastQCFastQC Report
Thu 26 May 2016
SRR937985_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937985_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2013548
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86770.4309308742577778No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69700.3461551450474486No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43810.21757613923283678No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT24050.1194409072939905No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67550.033.4197431
GTATCAA98100.030.601941
ATCAACG118050.025.075653
TATCAAC119000.025.035182
TCAACGC117750.025.0184764
CAACGCA118950.024.7261415
AACGCAG122800.023.9122376
GAGTACT81100.022.33205212-13
GTGTAAG7900.021.7491721
CGCAGAG134700.021.7052338
ACGCAGA134550.021.69417
GTACATG60150.021.5030941
GTACTTT86150.021.06479514-15
AGAGTAC108750.020.91775110-11
TAAGGTG12900.020.6266375
AGTACTT83650.020.51472912-13
TACATGG58650.020.4966372
ACATGGG59100.019.8581873
GCAGAGT147100.019.4554279
GTGTAGC13850.019.29771
Produced by FastQC (version 0.11.5)