FastQCFastQC Report
Thu 26 May 2016
SRR937985_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937985_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2013548
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81020.40237431638083626No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70060.3479430338884397No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43970.21837075649549947No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT25390.1260958268687908No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA20390.10126403741058072No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTA651.3289318E-436.536637
GGTATCA70550.028.9582751
GTATCAA101050.028.3048881
ATCAACG117200.024.114153
CAACGCA117450.024.1026635
TCAACGC117750.024.0009174
TATCAAC118850.023.9457742
AACGCAG120600.023.5518886
GAGTACT76150.022.45510912-13
GTACTTT78650.021.62055814-15
ACGCAGA131850.021.3622197
CGCAGAG133000.021.0703728
AGAGTAC100600.021.03424510-11
TGTACCG2801.5788828E-920.3561215
AGTACTT77750.020.34353612-13
GCAGAGT142550.019.4255439
TAGCCCT15700.019.3620854
TACATGG54400.018.9523072
GTATAGC10850.018.8295521
GTGTAAG9300.018.3916531
Produced by FastQC (version 0.11.5)