FastQCFastQC Report
Thu 26 May 2016
SRR937984_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937984_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1987771
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79600.4004485426138122No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67550.33982787755732424No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40350.20299118962898646No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT25080.12617147548686444No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA19980.10051459650030109No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68200.034.1986161
GTATCAA97450.030.576611
ATCAACG113800.025.911033
TATCAAC114550.025.8237692
TCAACGC114700.025.7077184
CAACGCA115600.025.5075725
AACGCAG118050.025.0933556
GAGTACT73100.023.5367912-13
TACATGG59700.023.1019152
GTACATG62300.022.997221
ACGCAGA130400.022.5658237
CGCAGAG130950.022.4699138
GTACTTT77100.022.0996514-15
ACATGGG60200.021.4885793
GGACCGA5100.021.4434366
GCAGAGT138700.020.7003049
AGAGTAC100650.020.28277210-11
GACCGTA1456.115436E-419.6707737
TAAGGTG12450.019.1001135
AGTACTT76450.019.08608812-13
Produced by FastQC (version 0.11.5)