##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937984_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1987771 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.023605837895815 33.0 31.0 34.0 30.0 34.0 2 32.14600373986742 34.0 31.0 34.0 30.0 34.0 3 32.171673195755446 34.0 31.0 34.0 30.0 34.0 4 35.68373721117775 37.0 35.0 37.0 35.0 37.0 5 35.68818842814389 37.0 35.0 37.0 35.0 37.0 6 35.721843713385496 37.0 36.0 37.0 35.0 37.0 7 35.65702336939215 37.0 35.0 37.0 33.0 37.0 8 35.65024190412276 37.0 35.0 37.0 33.0 37.0 9 37.2987637912013 39.0 37.0 39.0 34.0 39.0 10-11 37.20878561967148 39.0 37.0 39.0 34.0 39.0 12-13 37.06183609681397 39.0 37.0 39.0 33.5 39.0 14-15 38.246557073224224 40.0 38.0 41.0 33.5 41.0 16-17 38.0651644480174 40.0 38.0 41.0 33.0 41.0 18-19 38.25188817021679 40.0 38.0 41.0 33.5 41.0 20-21 38.251633865269184 40.0 38.0 41.0 34.0 41.0 22-23 38.138463635901715 40.0 38.0 41.0 33.5 41.0 24-25 38.01757118903536 40.0 38.0 41.0 33.0 41.0 26-27 37.83619315303423 40.0 38.0 41.0 33.0 41.0 28-29 37.65437593163398 40.0 38.0 41.0 32.0 41.0 30-31 37.42876241780366 40.0 37.0 41.0 32.0 41.0 32-33 37.12883828167329 40.0 36.5 41.0 31.0 41.0 34-35 36.88521363879441 39.5 36.0 41.0 30.5 41.0 36-37 36.609543805599344 39.0 36.0 41.0 30.0 41.0 38-39 36.29410907996947 39.0 35.0 40.5 30.0 41.0 40-41 36.169483305672536 39.0 35.0 41.0 30.0 41.0 42-43 35.94492122080461 39.0 35.0 40.5 28.5 41.0 44-45 35.60467931165109 39.0 35.0 40.0 27.0 41.0 46-47 35.219767015415755 38.5 35.0 40.0 25.5 41.0 48-49 34.80101505656336 38.0 34.0 40.0 24.0 41.0 50-51 34.070269412321636 37.5 33.5 39.5 23.5 40.5 52-53 34.140328790388836 38.0 33.5 40.0 23.0 40.5 54-55 34.213586726036354 38.0 34.0 40.0 23.0 41.0 56-57 33.88887100174014 38.0 33.5 40.0 21.5 41.0 58-59 33.46158838216273 37.5 33.0 40.0 19.0 41.0 60-61 32.94296777646922 37.0 32.5 40.0 15.0 41.0 62-63 32.38612999183508 36.0 31.5 39.0 10.0 41.0 64-65 31.83060649340392 36.0 31.0 39.0 7.5 40.5 66-67 31.22271931726542 35.0 30.5 39.0 2.0 40.0 68-69 30.52698575439525 35.0 29.0 38.0 2.0 40.0 70-71 29.905543193858847 34.5 28.5 37.5 2.0 39.5 72-73 29.183182821361214 34.0 28.0 37.0 2.0 39.0 74-75 28.483894271523226 34.0 26.0 36.0 2.0 39.0 76-77 27.706030272098747 33.5 25.5 35.5 2.0 38.5 78-79 26.915093086678496 33.0 24.5 35.0 2.0 37.0 80-81 26.15031132861884 32.0 22.0 35.0 2.0 37.0 82-83 25.376711904942773 31.5 19.0 35.0 2.0 36.0 84-85 24.612954912814402 31.0 15.5 34.5 2.0 36.0 86-87 23.832755634326087 31.0 7.0 34.0 2.0 35.0 88-89 22.906461056127693 30.0 2.0 34.0 2.0 35.0 90-91 21.96562204600027 29.0 2.0 34.0 2.0 35.0 92-93 20.990648570685458 29.0 2.0 34.0 2.0 35.0 94-95 19.867291302670175 26.5 2.0 33.5 2.0 35.0 96-97 18.556495692914325 25.0 2.0 33.0 2.0 35.0 98-99 16.96970174129716 12.5 2.0 32.5 2.0 34.0 100-101 13.109426337339663 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.15635981732090443 1101 2 0.14501571468218089 1101 3 0.05539682260444323 1101 4 9.718308931994102E-4 1101 5 0.0557071445646784 1101 6 0.06784875062088247 1101 7 0.0506112467065023 1101 8 0.0038819217827281705 1101 9 0.05920718713084483 1101 10-11 0.17596288856366726 1101 12-13 0.20940410579267876 1101 14-15 0.20510920505687125 1101 16-17 0.20762385118006677 1101 18-19 0.25452543626656876 1101 20-21 0.20154694389891858 1101 22-23 0.21054687408512507 1101 24-25 0.19416600708704124 1101 26-27 0.17219576934851233 1101 28-29 0.19020255835463473 1101 30-31 0.13866337253782746 1101 32-33 0.10769169384825261 1101 34-35 0.09723111998451373 1101 36-37 0.13851519427591796 1101 38-39 0.14539751217207453 1101 40-41 0.10554132477604838 1101 42-43 0.13565197537843687 1101 44-45 0.108002591438634 1101 46-47 0.16025525633191506 1101 48-49 0.37195039541592934 1101 50-51 0.3717553988700857 1101 52-53 0.2607913180003649 1101 54-55 0.22868540780269342 1101 56-57 0.23169852570892857 1101 58-59 0.16593172707106163 1101 60-61 0.19708898944661968 1101 62-63 0.15916516340607245 1101 64-65 -0.033907669788195705 1101 66-67 -0.11350929107931762 1101 68-69 0.012881370942796622 1101 70-71 -0.04533637970388327 1101 72-73 -0.058864823738456806 1101 74-75 0.01625059115915306 1101 76-77 -0.13513436270218548 1101 78-79 -0.20272588736142083 1101 80-81 -0.18271570914142643 1101 82-83 -0.31744899898690804 1101 84-85 -0.6836552401170763 1101 86-87 -0.5480337338373715 1101 88-89 -0.7033497332080714 1101 90-91 -0.6086696251582104 1101 92-93 -0.7292136939535183 1101 94-95 -1.0332110605368179 1101 96-97 -1.083846691596161 1101 98-99 -0.9908861018645894 1101 100-101 -1.2346739380749927 1102 1 0.14654716994684236 1102 2 0.12613554319236187 1102 3 0.09938567576628543 1102 4 0.12055854043232017 1102 5 0.056618612946238045 1102 6 0.11723095855228394 1102 7 0.1740882027622348 1102 8 0.21017216829933716 1102 9 0.2393821067449906 1102 10-11 0.13632859060011526 1102 12-13 0.22786755702527017 1102 14-15 0.3216935566109953 1102 16-17 0.17975075364523008 1102 18-19 0.26527699039304053 1102 20-21 0.327786600919282 1102 22-23 0.41964783871534905 1102 24-25 0.3597488581052559 1102 26-27 0.30513767717273055 1102 28-29 0.17394618107704218 1102 30-31 0.21191696310589236 1102 32-33 0.3586430336124593 1102 34-35 0.13739060551506554 1102 36-37 0.1866712500101002 1102 38-39 0.006137062011305261 1102 40-41 -0.012603584119979416 1102 42-43 0.11921982103759632 1102 44-45 0.27925489583306273 1102 46-47 0.2561446453994378 1102 48-49 0.16163699777391116 1102 50-51 0.19502399479720367 1102 52-53 0.20950857416328006 1102 54-55 0.048970509624780334 1102 56-57 0.10750388476572681 1102 58-59 -0.02171886242518184 1102 60-61 0.01971192620974449 1102 62-63 -0.0912991452755989 1102 64-65 -0.24031881662635257 1102 66-67 -0.2543633103719216 1102 68-69 -0.24760651758882446 1102 70-71 -0.3136206240768722 1102 72-73 -0.362481115271148 1102 74-75 -0.3596941033746077 1102 76-77 -0.6313157131845024 1102 78-79 -0.5699083096550979 1102 80-81 -0.5150728366869757 1102 82-83 -0.6298365659751184 1102 84-85 -0.5106464512639164 1102 86-87 -0.5211895752092914 1102 88-89 -0.43142090148245416 1102 90-91 -0.4024484032932598 1102 92-93 -0.3213753231067855 1102 94-95 -0.5132980916193439 1102 96-97 -0.5675225758405347 1102 98-99 -0.6547887813929911 1102 100-101 -0.46053460259803636 1103 1 0.2836735059311408 1103 2 0.3660180135327593 1103 3 0.2863126010788406 1103 4 0.16782407749717265 1103 5 0.28087078092831064 1103 6 0.27476034936696436 1103 7 0.2837523125372172 1103 8 0.31436802418606646 1103 9 0.34766361346312635 1103 10-11 0.3685697851153975 1103 12-13 0.24799929910512475 1103 14-15 0.2581215352554054 1103 16-17 0.31405812495227536 1103 18-19 0.3996297204880932 1103 20-21 0.4138733806805206 1103 22-23 0.4138956724132328 1103 24-25 0.4310679924579901 1103 26-27 0.327471840403895 1103 28-29 0.4027325479053232 1103 30-31 0.4555415334573638 1103 32-33 0.4327391337646631 1103 34-35 0.1945510781872315 1103 36-37 0.10208719009618505 1103 38-39 0.07071621645629733 1103 40-41 0.19121864974993485 1103 42-43 0.2709029680639077 1103 44-45 0.11797500523174165 1103 46-47 0.17547908078332597 1103 48-49 0.040843185384581204 1103 50-51 0.05943397776245973 1103 52-53 0.016802603266825145 1103 54-55 -0.03384959742195548 1103 56-57 0.03450479530140882 1103 58-59 0.07929431850262603 1103 60-61 -0.10754131358368824 1103 62-63 -0.0031888595824298704 1103 64-65 -0.07272313478296866 1103 66-67 -0.14566937467388286 1103 68-69 -0.2260745329234517 1103 70-71 -0.4699202877498614 1103 72-73 -0.346112472641277 1103 74-75 -0.43025473799444924 1103 76-77 -0.4404518130679129 1103 78-79 -0.5719158560134616 1103 80-81 -0.6826601187548 1103 82-83 -0.7606051118395705 1103 84-85 -1.0960284898558434 1103 86-87 -1.2527856669617172 1103 88-89 -0.9611779463742138 1103 90-91 -1.1206888519084721 1103 92-93 -1.2060237253014812 1103 94-95 -1.154867068896273 1103 96-97 -1.2100205682941763 1103 98-99 -1.4665128521258222 1103 100-101 -1.5134221094438551 1104 1 0.1345227346295843 1104 2 0.21324540137966608 1104 3 0.20502928279961452 1104 4 0.1292767101946879 1104 5 0.24183636901254602 1104 6 0.1355541435330565 1104 7 0.10748643931050594 1104 8 0.22088202450485994 1104 9 0.33314082863058303 1104 10-11 0.25745277043321124 1104 12-13 0.21601817872539897 1104 14-15 0.33152358615599553 1104 16-17 0.34300628569470604 1104 18-19 0.2511873777149489 1104 20-21 0.2210612736369697 1104 22-23 0.30815385919041205 1104 24-25 0.3362713999992124 1104 26-27 0.19707671439216767 1104 28-29 0.19465823375268343 1104 30-31 0.19437492877820262 1104 32-33 0.26346899225605824 1104 34-35 0.17042023143802965 1104 36-37 0.3535261855445313 1104 38-39 0.16510989018954092 1104 40-41 0.08123069303491803 1104 42-43 0.2567609635653838 1104 44-45 0.19765107628711576 1104 46-47 0.07194667903350904 1104 48-49 0.06561161780935265 1104 50-51 -0.09011085690803355 1104 52-53 0.09363610485193163 1104 54-55 0.06190780122847883 1104 56-57 -0.1291244840496759 1104 58-59 0.09492402291081703 1104 60-61 0.048082723396284166 1104 62-63 0.03432243099724275 1104 64-65 -0.1521379728184975 1104 66-67 0.01754813059505267 1104 68-69 0.08507613211383003 1104 70-71 -0.2484965235148735 1104 72-73 -0.06791258953198209 1104 74-75 -0.23947493529179908 1104 76-77 -0.4825944551521104 1104 78-79 -0.5785352350758544 1104 80-81 -0.5348332232657214 1104 82-83 -0.5807661022226576 1104 84-85 -0.5355685426337686 1104 86-87 -0.5437859680438457 1104 88-89 -0.5774399232440217 1104 90-91 -0.8741911454458311 1104 92-93 -1.1697433292899362 1104 94-95 -1.3217374087648572 1104 96-97 -1.515632207774896 1104 98-99 -1.5593302723833347 1104 100-101 -1.6329030084396567 1105 1 0.15865142164343382 1105 2 0.12406542374286289 1105 3 0.08378053000261332 1105 4 0.02250998550666594 1105 5 0.03685959676749917 1105 6 8.97462175331043E-4 1105 7 0.1475888028162231 1105 8 0.10923587024490189 1105 9 0.21017244066367624 1105 10-11 0.09333545714221714 1105 12-13 0.11717002432967405 1105 14-15 0.054511698822452104 1105 16-17 0.16874637819585558 1105 18-19 0.13213075629981574 1105 20-21 0.1049431201250215 1105 22-23 0.2621837818157928 1105 24-25 0.22031796053673958 1105 26-27 0.18366443102764407 1105 28-29 0.08186074621831807 1105 30-31 0.046651402463012914 1105 32-33 0.09279693075598061 1105 34-35 0.1570322837417848 1105 36-37 0.15343921755106038 1105 38-39 0.1080907790049963 1105 40-41 0.26952536467630495 1105 42-43 0.36128855060702847 1105 44-45 0.38071614089831485 1105 46-47 0.36696913829367617 1105 48-49 0.4088760812014627 1105 50-51 0.3431050248052472 1105 52-53 0.17576322157476199 1105 54-55 0.3222149173621318 1105 56-57 0.18800974765406409 1105 58-59 0.3178452765307469 1105 60-61 0.17944576417645663 1105 62-63 0.2725556870968475 1105 64-65 0.12695218058587088 1105 66-67 0.007763526875795179 1105 68-69 -0.10717116438554797 1105 70-71 0.016336770753309082 1105 72-73 -0.16583406729622752 1105 74-75 -0.20770817858315738 1105 76-77 -0.42404142837134984 1105 78-79 -0.3976213154578474 1105 80-81 -0.503735825517154 1105 82-83 -0.3801269707242305 1105 84-85 -0.48720037727418486 1105 86-87 -0.48651286933197113 1105 88-89 -0.7162893840808877 1105 90-91 -0.9498247373776643 1105 92-93 -1.3240838518920057 1105 94-95 -1.446344227619111 1105 96-97 -1.5367923275063866 1105 98-99 -1.3766709730200333 1105 100-101 -1.6827603886153089 1106 1 0.18189662616766356 1106 2 0.20043404011661892 1106 3 0.17523347347331963 1106 4 0.165520646059079 1106 5 0.21046291075740697 1106 6 0.11080072534441854 1106 7 0.21538849789133252 1106 8 0.01654195337830089 1106 9 0.1656182455826567 1106 10-11 0.20534867055418715 1106 12-13 0.2381148482887383 1106 14-15 0.3040874041089978 1106 16-17 0.04187250836805845 1106 18-19 0.40013949629815926 1106 20-21 0.46423904500476 1106 22-23 0.3921956892510039 1106 24-25 0.28782129934612044 1106 26-27 0.275836843598114 1106 28-29 0.25730808758054735 1106 30-31 0.18449370429138412 1106 32-33 0.2799509355544174 1106 34-35 0.24219762867329564 1106 36-37 0.24960460975774623 1106 38-39 0.26803969226686064 1106 40-41 0.15548729634003422 1106 42-43 8.19905868162607E-4 1106 44-45 0.3270403481526927 1106 46-47 0.297849727106005 1106 48-49 0.23499162764341008 1106 50-51 0.21989852683216782 1106 52-53 0.16664945386134633 1106 54-55 0.0863686621470876 1106 56-57 0.08183501859992504 1106 58-59 0.10812682975668508 1106 60-61 0.3821991000311371 1106 62-63 0.2350921776240824 1106 64-65 0.18969564774735304 1106 66-67 0.04362056674058934 1106 68-69 -0.09587091973650885 1106 70-71 -0.3790081016628086 1106 72-73 -0.3674137968822144 1106 74-75 -0.32609364264811447 1106 76-77 -0.6352010293688544 1106 78-79 -0.6431464244862255 1106 80-81 -0.8002025969771296 1106 82-83 -0.7306363607562609 1106 84-85 -0.715906898884846 1106 86-87 -0.5529340497931408 1106 88-89 -0.8637374109331901 1106 90-91 -1.1293222317932816 1106 92-93 -1.139236284790801 1106 94-95 -1.3714830984515096 1106 96-97 -1.4848689664776806 1106 98-99 -1.8038816784838616 1106 100-101 -1.6475736221192268 1107 1 -0.03415258039408897 1107 2 0.06701837023258861 1107 3 0.04208670989793717 1107 4 0.15149114539065067 1107 5 0.07449016139345588 1107 6 0.13846761738018643 1107 7 0.14268720779959665 1107 8 0.0861587783521287 1107 9 0.07162972843082827 1107 10-11 0.06582745549280133 1107 12-13 0.09411949872150416 1107 14-15 0.21544436022129787 1107 16-17 0.21663938306210895 1107 18-19 0.15889363141864266 1107 20-21 0.14818124990478765 1107 22-23 0.24068934745242387 1107 24-25 0.29309872285050886 1107 26-27 0.17789458052652662 1107 28-29 0.15667209672116655 1107 30-31 0.1588990371973935 1107 32-33 0.20621174942953502 1107 34-35 0.09814314715758599 1107 36-37 0.15915166177629914 1107 38-39 0.03949663213501964 1107 40-41 0.2147578138913815 1107 42-43 0.14517910213464802 1107 44-45 0.12263739150039044 1107 46-47 0.13120341134195002 1107 48-49 -0.06370277734924201 1107 50-51 -0.13734103466395453 1107 52-53 -0.13944632288275471 1107 54-55 -0.1592343081151668 1107 56-57 -0.2792013353378522 1107 58-59 -0.3013728892636536 1107 60-61 -0.1335213548485754 1107 62-63 -0.2167003298778596 1107 64-65 -0.08949673880162479 1107 66-67 -0.23477209793434284 1107 68-69 -0.20298535748085555 1107 70-71 -0.20175521558469356 1107 72-73 -0.12779426638165248 1107 74-75 -0.20254230678742502 1107 76-77 -0.4049439659135494 1107 78-79 -0.592612069235745 1107 80-81 -0.4787547395567415 1107 82-83 -0.21403606089828742 1107 84-85 -0.5024483850916006 1107 86-87 -0.49641008091163386 1107 88-89 -0.7938264353850073 1107 90-91 -0.9139909776362103 1107 92-93 -1.0048041216206514 1107 94-95 -1.2365077719772977 1107 96-97 -1.285116168205704 1107 98-99 -1.4321787991009636 1107 100-101 -1.5237478920660372 1108 1 0.07507809689079892 1108 2 -0.06916923155437615 1108 3 0.036372552251137336 1108 4 0.15233404287477015 1108 5 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6548.0 3 1817.0 4 1636.0 5 1878.0 6 2261.0 7 2855.0 8 3652.0 9 4370.0 10 5713.0 11 7272.0 12 9428.0 13 11631.0 14 13921.0 15 17153.0 16 19984.0 17 24646.0 18 24540.0 19 21766.0 20 21768.0 21 23395.0 22 25490.0 23 28796.0 24 32340.0 25 35878.0 26 41117.0 27 47174.0 28 53887.0 29 62517.0 30 72121.0 31 85914.0 32 103206.0 33 127880.0 34 162485.0 35 208973.0 36 260356.0 37 271043.0 38 134601.0 39 7759.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.33136356102592 18.652836950711862 14.971025203513753 23.04477428474847 2 15.214888824573103 19.631755778983 40.932690734751915 24.22066466169198 3 19.44700985571388 23.520899746414763 29.811053051361462 27.221037346509892 4 12.736647664162945 16.694833760415317 37.431322244817466 33.137196330604276 5 13.731173110355149 37.03480615001227 34.47977777452946 14.75424296510312 6 32.17475113468871 36.19430145316775 17.236733064355146 14.39421434778839 7 28.358074324766957 32.812914162584036 20.380351799462414 18.448659713186583 8 26.02030978692898 35.04175785228358 19.530662784135387 19.40726957665205 9 25.74667067611001 17.272037205216506 20.45702617169182 36.52426594698166 10-11 24.903781194470387 26.57071770604799 27.13396754861048 21.391533550871138 12-13 26.05948124901906 23.84580715716834 27.50828269829917 22.586428895513425 14-15 22.60819296774047 25.44852757815511 26.19784936485986 25.745430089244557 16-17 21.98557300674197 28.51227866192383 27.048975103134516 22.453173228199688 18-19 21.857033937431723 27.081711337001867 29.342804658453225 21.71845006711318 20-21 22.40532722634415 26.157503496137117 29.779355461429645 21.65781381608908 22-23 22.148335404258965 26.00907846749823 29.700450898899344 22.142135229343463 24-25 22.105363991918153 26.144043923300604 29.76496849566981 21.985623589111437 26-27 21.653908880537646 26.449830486784315 29.720334683426703 22.175925949251337 28-29 21.69557248686097 27.025407620296377 29.445980944599604 21.833038948243047 30-31 21.90766063606817 26.443803436665625 29.347927898710925 22.300608028555285 32-33 21.660927680717613 26.454424929429614 29.394782123377787 22.489865266474986 34-35 22.444404383394986 26.56219628996635 29.190959273019523 21.80244005361914 36-37 22.40165680467414 26.125690870278145 29.26651498760191 22.206137337445806 38-39 21.92656230456014 26.590215102447296 29.158689679315668 22.324532913676894 40-41 22.411984845277715 26.45653286270771 29.06406023833476 22.06742205367981 42-43 22.097741501338174 26.38971705904103 28.910514582492752 22.602026857128045 44-45 22.163400492102852 26.72433533653786 28.95668961435503 22.15557455700426 46-47 22.56958137795427 26.926812301982082 28.30994596371256 22.19366035635108 48-49 22.365767171814145 26.92903030658386 28.26716237738822 22.438040144213772 50-51 22.362067672446553 27.21147501128747 28.30810830128297 22.11834901498301 52-53 22.70485358480407 27.000504467874453 28.072165324564065 22.222476622757416 54-55 22.236687364431234 27.083729181331396 28.27828204443548 22.401301409801896 56-57 22.42679249688511 27.12164884955325 28.111947899284832 22.339610754276812 58-59 22.523017467706087 26.877989804464775 28.04187437586573 22.557118351963407 60-61 22.164620421552243 27.110887772025706 28.31291785385454 22.411573952567508 62-63 22.162193858701613 27.077190541719983 28.492268261770754 22.26834733780765 64-65 21.944109133468366 27.224533540282486 28.24460866503956 22.58674866120959 66-67 21.98007873088471 27.118245293710892 28.099890187426208 22.80178578797819 68-69 22.42784608507245 26.817364461591747 28.29077327974354 22.46401617359226 70-71 22.336911425052985 26.939655660580364 27.917521424632685 22.805911489733973 72-73 22.343785076694328 26.886679438148747 28.04650685200416 22.72302863315277 74-75 22.221548354170956 27.425434263220914 27.725428575886408 22.627588806721725 76-77 22.423892934966794 26.94931932046019 27.839842239443108 22.78694550512991 78-79 22.529944093289057 27.138970733826845 27.801921021109493 22.529164151774605 80-81 22.70807764092206 27.188588658402992 27.448445113375207 22.654888587299745 82-83 22.636879132137317 27.18401021677949 27.650568429124867 22.52854222195833 84-85 22.604760364079343 27.37747745889163 27.494868844185454 22.522893332843573 86-87 22.761669720657345 27.30972145239964 27.26564133426895 22.66296749267407 88-89 22.69139409035751 27.360117879725525 27.118629562610135 22.829858467306828 90-91 22.70805995045679 27.291197178399116 27.07873603118951 22.92200683995458 92-93 22.830601684429414 26.982590537136875 27.341730725417364 22.845077053016354 94-95 22.65733364414319 26.947994898824845 27.320110824188188 23.074560632843774 96-97 22.848240729413504 26.64540670562229 27.433681557701185 23.072671007263025 98-99 22.721012621526363 26.854016347479064 26.956150338819075 23.4688206921755 100-101 23.699680974697106 25.625645745397357 27.09929278442645 23.575380495479088 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 531.0 1 376.5 2 299.5 3 376.0 4 317.5 5 320.0 6 577.5 7 1290.5 8 2210.0 9 2652.5 10 2273.0 11 1722.5 12 1651.0 13 1829.5 14 2003.5 15 2186.0 16 2434.5 17 2611.5 18 2824.5 19 3219.5 20 3722.5 21 4384.0 22 5348.5 23 6698.0 24 8377.0 25 10406.5 26 13022.0 27 16119.0 28 18936.5 29 21844.0 30 24980.0 31 28854.5 32 33768.0 33 36233.0 34 37725.5 35 40732.0 36 44537.0 37 48420.0 38 51291.5 39 53606.0 40 56526.5 41 60002.0 42 62321.5 43 64055.0 44 66311.0 45 69439.5 46 71424.5 47 73813.0 48 76668.5 49 75068.5 50 71443.0 51 68908.5 52 67086.5 53 66445.5 54 66442.5 55 63174.5 56 57591.0 57 52408.5 58 48318.5 59 43311.0 60 35912.0 61 29727.5 62 24662.0 63 19044.0 64 14125.5 65 10728.0 66 8127.5 67 6208.0 68 4792.0 69 3689.5 70 2750.5 71 1943.5 72 1347.5 73 1030.5 74 773.5 75 546.0 76 380.0 77 250.0 78 173.5 79 115.5 80 76.5 81 45.0 82 26.5 83 22.5 84 17.5 85 10.0 86 11.0 87 8.5 88 3.5 89 3.0 90 3.0 91 2.5 92 1.5 93 1.0 94 1.0 95 0.5 96 0.5 97 1.0 98 1.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13935206822113813 2 0.05262175572538285 3 0.15263327616712388 4 0.13049792959048098 5 0.19318120648706516 6 0.199570272430778 7 0.16827894158834192 8 0.07234233722093743 9 0.12365609519406409 10-11 0.10375943707801351 12-13 0.15401673532816407 14-15 0.13965391385627418 16-17 0.13794345525716997 18-19 0.13540292116144165 20-21 0.13698761074590585 22-23 0.19901688876636192 24-25 0.21700185785988427 26-27 0.14941358939233948 28-29 0.12876231718844877 30-31 0.10106798016471716 32-33 0.1267248591512805 34-35 0.09623845000254054 36-37 0.08952238462076366 38-39 0.053653061645430995 40-41 0.06014274280085584 42-43 0.06522381099231249 44-45 0.03964239341453316 46-47 0.05284813995173488 48-49 0.04323938723323763 50-51 0.0530242165722309 52-53 0.02658756969489946 54-55 0.050433374870646565 56-57 0.027317029979811558 58-59 0.051263450367270674 60-61 0.03810801143592496 62-63 0.05156529600240672 64-65 0.040724006940437305 66-67 0.03768039678614891 68-69 0.06655696254749667 70-71 0.055212597426967186 72-73 0.0529739089663749 74-75 0.045276845270405895 76-77 0.022915114467410985 78-79 0.0222611155912829 80-81 0.026159955045123407 82-83 0.022739037846914962 84-85 0.020223657554114635 86-87 0.024172804613811147 88-89 0.06738703804412077 90-91 0.11226142246767862 92-93 0.08242901219506674 94-95 0.07858048034708223 96-97 0.07083310904525723 98-99 0.058608360822247636 100-101 0.13391884678868946 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1987771.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.22176832937177 #Duplication Level Percentage of deduplicated Percentage of total 1 79.18772171660916 48.48012353465008 2 12.384198042834328 15.163650070469254 3 3.8312058694574525 7.036595944861607 4 1.6070695440683642 3.9355055726457007 5 0.8719380467950976 2.669079454922719 6 0.49203557039684115 1.8073972620387422 7 0.3180227060065406 1.3628938701428635 8 0.22920006229673223 1.1225626492006495 9 0.1678515031707721 0.924854925677204 >10 0.8355832453117829 9.092668272647748 >50 0.04517747647548704 1.900998033621738 >100 0.02505357416152587 2.894239275425622 >500 0.00337747231753144 1.449138512423318 >1k 0.0014004153511715655 1.4181727211437165 >5k 1.6475474719665476E-4 0.742119900129082 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7960 0.4004485426138122 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6755 0.33982787755732424 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4035 0.20299118962898646 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2508 0.12617147548686444 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 1998 0.10051459650030109 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.030760585600655E-5 2 0.0 0.0 0.0 0.0 5.030760585600655E-5 3 0.0 0.0 0.0 0.0 5.030760585600655E-5 4 0.0 0.0 0.0 5.030760585600655E-5 5.030760585600655E-5 5 0.0 0.0 0.0 5.030760585600655E-5 5.030760585600655E-5 6 0.0 0.0 0.0 5.030760585600655E-5 5.030760585600655E-5 7 0.0 0.0 0.0 5.030760585600655E-5 5.030760585600655E-5 8 0.0 0.0 0.0 5.030760585600655E-5 5.030760585600655E-5 9 0.0 0.0 0.0 5.030760585600655E-5 5.030760585600655E-5 10-11 0.0 5.030760585600655E-5 0.0 1.2576901464001638E-4 5.030760585600655E-5 12-13 0.0 1.5092281756801966E-4 0.0 1.5092281756801966E-4 5.030760585600655E-5 14-15 0.0 1.5092281756801966E-4 0.0 2.012304234240262E-4 5.030760585600655E-5 16-17 0.0 1.5092281756801966E-4 0.0 2.012304234240262E-4 1.006152117120131E-4 18-19 5.030760585600655E-5 1.5092281756801966E-4 0.0 2.012304234240262E-4 2.012304234240262E-4 20-21 7.546140878400982E-5 1.5092281756801966E-4 0.0 2.5153802928003276E-4 2.012304234240262E-4 22-23 1.006152117120131E-4 1.5092281756801966E-4 0.0 3.018456351360393E-4 2.012304234240262E-4 24-25 1.006152117120131E-4 1.5092281756801966E-4 0.0 4.2761464977605565E-4 2.2638422635202948E-4 26-27 1.006152117120131E-4 1.5092281756801966E-4 0.0 8.552292995521113E-4 2.5153802928003276E-4 28-29 1.006152117120131E-4 2.012304234240262E-4 0.0 0.003571840015776465 2.5153802928003276E-4 30-31 1.006152117120131E-4 2.012304234240262E-4 0.0 0.011319211317601476 2.5153802928003276E-4 32-33 1.006152117120131E-4 2.012304234240262E-4 0.0 0.028524412520355716 2.5153802928003276E-4 34-35 1.006152117120131E-4 2.012304234240262E-4 0.0 0.05312483178394292 2.5153802928003276E-4 36-37 1.5092281756801966E-4 2.012304234240262E-4 0.0 0.09314453224239613 2.5153802928003276E-4 38-39 1.5092281756801966E-4 2.012304234240262E-4 0.0 0.15104858658265968 3.521532409920459E-4 40-41 1.5092281756801966E-4 2.2638422635202948E-4 0.0 0.2094557169814833 3.521532409920459E-4 42-43 1.5092281756801966E-4 2.5153802928003276E-4 0.0 0.26884384569449904 3.521532409920459E-4 44-45 1.5092281756801966E-4 2.5153802928003276E-4 0.0 0.34375187081409275 3.521532409920459E-4 46-47 1.5092281756801966E-4 2.5153802928003276E-4 0.0 0.4292999545722319 3.521532409920459E-4 48-49 1.5092281756801966E-4 2.5153802928003276E-4 0.0 0.5209855662448039 4.024608468480524E-4 50-51 1.5092281756801966E-4 2.5153802928003276E-4 0.0 0.6160920951155842 4.5276845270405896E-4 52-53 1.5092281756801966E-4 2.5153802928003276E-4 0.0 0.7074758611530201 4.5276845270405896E-4 54-55 1.5092281756801966E-4 2.5153802928003276E-4 0.0 0.8073867663830492 4.5276845270405896E-4 56-57 1.5092281756801966E-4 2.5153802928003276E-4 0.0 0.9277477133935448 4.5276845270405896E-4 58-59 1.5092281756801966E-4 2.5153802928003276E-4 0.0 1.0472031234986323 4.5276845270405896E-4 60-61 1.5092281756801966E-4 3.018456351360393E-4 0.0 1.1776758992861853 4.5276845270405896E-4 62-63 1.5092281756801966E-4 3.521532409920459E-4 0.0 1.3015332249036735 4.5276845270405896E-4 64-65 1.5092281756801966E-4 4.024608468480524E-4 0.0 1.4321820773117224 4.5276845270405896E-4 66-67 1.5092281756801966E-4 4.024608468480524E-4 0.0 1.5873558875745748 4.5276845270405896E-4 68-69 1.5092281756801966E-4 4.024608468480524E-4 0.0 1.757772902411797 4.5276845270405896E-4 70-71 1.5092281756801966E-4 4.2761464977605565E-4 0.0 1.9388299758875644 4.5276845270405896E-4 72-73 1.5092281756801966E-4 5.030760585600655E-4 0.0 2.1153342110333635 5.030760585600655E-4 74-75 2.012304234240262E-4 5.030760585600655E-4 0.0 2.2943035188661067 5.282298614880688E-4 76-77 2.012304234240262E-4 5.030760585600655E-4 0.0 2.4880381090175883 5.53383664416072E-4 78-79 2.012304234240262E-4 5.030760585600655E-4 0.0 2.7005625899562875 5.53383664416072E-4 80-81 3.7730704392004914E-4 5.030760585600655E-4 0.0 2.929110043360126 5.53383664416072E-4 82-83 4.5276845270405896E-4 5.282298614880688E-4 0.0 3.1598207238157716 6.036912702720786E-4 84-85 4.5276845270405896E-4 5.53383664416072E-4 0.0 3.393348630199354 6.036912702720786E-4 86-87 4.5276845270405896E-4 5.53383664416072E-4 0.0 3.626297999115592 6.036912702720786E-4 88-89 4.5276845270405896E-4 5.53383664416072E-4 0.0 3.8778611821985534 6.539988761280852E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6820 0.0 34.198616 1 GTATCAA 9745 0.0 30.57661 1 ATCAACG 11380 0.0 25.91103 3 TATCAAC 11455 0.0 25.823769 2 TCAACGC 11470 0.0 25.707718 4 CAACGCA 11560 0.0 25.507572 5 AACGCAG 11805 0.0 25.093355 6 GAGTACT 7310 0.0 23.53679 12-13 TACATGG 5970 0.0 23.101915 2 GTACATG 6230 0.0 22.99722 1 ACGCAGA 13040 0.0 22.565823 7 CGCAGAG 13095 0.0 22.469913 8 GTACTTT 7710 0.0 22.09965 14-15 ACATGGG 6020 0.0 21.488579 3 GGACCGA 510 0.0 21.443436 6 GCAGAGT 13870 0.0 20.700304 9 AGAGTAC 10065 0.0 20.282772 10-11 GACCGTA 145 6.115436E-4 19.670773 7 TAAGGTG 1245 0.0 19.100113 5 AGTACTT 7645 0.0 19.086088 12-13 >>END_MODULE