FastQCFastQC Report
Thu 26 May 2016
SRR937984_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937984_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1987771
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76190.38329364901691393No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67650.34033095361588434No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41770.21013486966053938No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT24650.12400824843505616No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61450.033.7012021
GTATCAA86450.030.3837221
ATCAACG103600.025.2126333
TCAACGC104300.025.0883244
TATCAAC105100.024.8540462
CAACGCA105850.024.7209455
AACGCAG108650.024.1275776
GTACATG55550.022.1460631
GAGTACT74000.022.1407212-13
TACATGG53600.021.8861472
ACGCAGA120950.021.5163277
CGCAGAG121000.021.3896948
TAAGGTG11150.020.8701525
GTACTTT78650.020.77131814-15
AGAGTAC99150.020.66745410-11
ACATGGG54250.020.6598683
TATACCG1150.00372865620.647935
GTAAGGT11000.019.8595544
GTGTAGC16200.019.6444841
AGTACTT78050.019.37942512-13
Produced by FastQC (version 0.11.5)