##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937984_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1987771 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.45851609667311 33.0 31.0 34.0 31.0 34.0 2 32.68323966895583 34.0 31.0 34.0 31.0 34.0 3 32.739769822580165 34.0 31.0 34.0 31.0 34.0 4 36.207665772365125 37.0 35.0 37.0 35.0 37.0 5 36.179652988196324 37.0 35.0 37.0 35.0 37.0 6 36.21099060203615 37.0 37.0 37.0 35.0 37.0 7 36.1692785537167 37.0 37.0 37.0 35.0 37.0 8 36.15295323254037 37.0 36.0 37.0 35.0 37.0 9 37.83671308214075 39.0 38.0 39.0 35.0 39.0 10-11 37.80558323871311 39.0 38.0 39.0 35.0 39.0 12-13 37.725516168612984 39.0 38.0 39.0 35.0 39.0 14-15 38.977852328059925 40.0 38.0 41.0 35.5 41.0 16-17 38.85109351127469 40.0 38.0 41.0 35.0 41.0 18-19 39.115249694255525 40.0 38.5 41.0 36.0 41.0 20-21 39.09436298245623 40.0 39.0 41.0 36.0 41.0 22-23 38.995446407055944 40.0 38.5 41.0 35.0 41.0 24-25 38.89073414392302 40.0 38.0 41.0 35.0 41.0 26-27 38.70233039922607 40.0 38.0 41.0 34.5 41.0 28-29 38.58038350494096 40.0 38.0 41.0 34.0 41.0 30-31 38.332893225628105 40.0 38.0 41.0 34.0 41.0 32-33 38.108080608883014 40.0 38.0 41.0 34.0 41.0 34-35 37.942283341491546 40.0 37.0 41.0 33.0 41.0 36-37 37.92421184331596 40.0 37.0 41.0 33.0 41.0 38-39 37.76658478265354 40.0 37.0 41.0 33.0 41.0 40-41 37.58622195413858 40.0 37.0 41.0 33.0 41.0 42-43 37.343367269167324 40.0 37.0 41.0 32.0 41.0 44-45 37.058317079784345 40.0 36.0 41.0 31.0 41.0 46-47 36.657720632809315 39.0 36.0 41.0 30.5 41.0 48-49 36.2412486146543 39.0 35.0 41.0 30.0 41.0 50-51 35.83248321864038 39.0 35.0 40.0 28.5 41.0 52-53 35.28610312757355 38.0 34.5 40.0 27.0 41.0 54-55 34.94517804113251 38.0 34.0 40.0 26.0 41.0 56-57 34.56233489672603 38.0 34.0 40.0 26.0 41.0 58-59 34.092334076712056 37.5 33.5 40.0 24.0 41.0 60-61 33.56794721323533 37.0 33.0 39.5 23.0 41.0 62-63 33.069123656598265 36.0 32.0 39.0 21.5 40.5 64-65 32.54612830149952 36.0 31.5 39.0 19.5 40.0 66-67 31.950630882531236 35.0 31.0 38.5 17.5 40.0 68-69 31.311238316687387 35.0 30.0 38.0 12.0 40.0 70-71 30.63825184088107 34.5 29.0 37.0 8.0 39.5 72-73 29.998621320061517 34.0 29.0 36.5 7.0 39.0 74-75 29.362715825917576 34.0 28.0 36.0 2.0 39.0 76-77 28.031438732127594 31.5 26.5 34.5 2.0 36.5 78-79 28.694673330076753 33.5 28.0 35.0 2.0 37.0 80-81 28.323241962982657 33.5 27.0 35.0 2.0 37.0 82-83 27.694816958291476 33.0 26.0 35.0 2.0 36.0 84-85 27.032745220651677 32.5 25.5 35.0 2.0 36.0 86-87 26.316482884597875 32.0 24.5 35.0 2.0 35.5 88-89 25.481962710996385 31.5 21.0 34.0 2.0 35.0 90-91 24.858010304003834 31.0 19.0 34.0 2.0 35.0 92-93 23.950257348557756 31.0 11.0 34.0 2.0 35.0 94-95 23.019327930631846 30.0 2.0 34.0 2.0 35.0 96-97 21.855971588276518 29.5 2.0 34.0 2.0 35.0 98-99 20.29889987327514 29.0 2.0 34.0 2.0 35.0 100-101 16.094130561317172 13.5 2.0 30.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1170604564238431 1101 2 0.032748604037514895 1101 3 0.0027396129283019377 1101 4 0.03539478501042481 1101 5 0.04399666335929453 1101 6 0.05987967864987809 1101 7 0.05300005786295969 1101 8 0.11506616044639628 1101 9 -0.08074114142389277 1101 10-11 0.03530864041393755 1101 12-13 0.0962204771505597 1101 14-15 0.11715444284653387 1101 16-17 0.11964576430586504 1101 18-19 0.20188212505913583 1101 20-21 0.17676969736116632 1101 22-23 0.18674272085522858 1101 24-25 0.20280335684766015 1101 26-27 0.1782761335538936 1101 28-29 0.12769160890153586 1101 30-31 0.22779085039766045 1101 32-33 0.11480620408611486 1101 34-35 0.16681096386128047 1101 36-37 0.24291944601137772 1101 38-39 0.20801838841469333 1101 40-41 0.13067017678031334 1101 42-43 0.35592920718329424 1101 44-45 0.29149959843881845 1101 46-47 0.26363121489198704 1101 48-49 0.34100905107884927 1101 50-51 0.2951107443217609 1101 52-53 -0.018253938810488535 1101 54-55 -0.0767757271848879 1101 56-57 0.1587320561190424 1101 58-59 0.19291063468431702 1101 60-61 0.16693608040866081 1101 62-63 0.24773982000048989 1101 64-65 0.2203245175234798 1101 66-67 -0.05240240933185447 1101 68-69 0.15545045198357244 1101 70-71 0.21158308494197087 1101 72-73 0.20872084598249074 1101 74-75 0.21371473129289598 1101 76-77 0.08320983813394278 1101 78-79 0.07981452826448532 1101 80-81 0.09186897460266508 1101 82-83 0.2795936833539372 1101 84-85 0.17047590365157816 1101 86-87 0.05014227828414164 1101 88-89 -0.24879299702586266 1101 90-91 -0.4635883778830632 1101 92-93 -0.4822302097926361 1101 94-95 -0.7444256297610892 1101 96-97 -0.7325770011751516 1101 98-99 -0.8702821497405573 1101 100-101 -1.5378578528394797 1102 1 0.1745344114077625 1102 2 0.15788472408038245 1102 3 0.0862088519422386 1102 4 0.053196285546327715 1102 5 0.10563696346647333 1102 6 0.034963065573784036 1102 7 0.1103685466089388 1102 8 0.13941428477651385 1102 9 0.11367600755788487 1102 10-11 0.16608506056399364 1102 12-13 0.14789035925131344 1102 14-15 0.17017866578330398 1102 16-17 0.1771604481215121 1102 18-19 0.17076615506985604 1102 20-21 0.17301750014787842 1102 22-23 0.16941839073732723 1102 24-25 0.31983100958078836 1102 26-27 0.247107430445638 1102 28-29 0.2806330880012098 1102 30-31 0.20238763496357848 1102 32-33 0.19118648275706818 1102 34-35 0.29677495099954854 1102 36-37 0.2879393817026923 1102 38-39 0.23230306151222635 1102 40-41 0.3268408091490116 1102 42-43 0.3697632907845758 1102 44-45 0.3239746252341007 1102 46-47 0.36164193300560044 1102 48-49 0.5164131239620247 1102 50-51 0.435143327387145 1102 52-53 0.5437374866986247 1102 54-55 0.5716941549158605 1102 56-57 0.4420310914888148 1102 58-59 0.5539258544056409 1102 60-61 0.4279873129917249 1102 62-63 0.43601806222986284 1102 64-65 0.4054237672555274 1102 66-67 0.5920829068524931 1102 68-69 0.5505702804937549 1102 70-71 0.3541622918015648 1102 72-73 0.5148901921775568 1102 74-75 0.26312823611604585 1102 76-77 0.31211787671915303 1102 78-79 0.405197164920434 1102 80-81 0.3776876241203482 1102 82-83 0.29724513029927735 1102 84-85 0.11420795081127721 1102 86-87 -0.021397700279631948 1102 88-89 -0.32119128212768544 1102 90-91 -0.17316565548220453 1102 92-93 -0.20197083144323003 1102 94-95 -0.1336693596646228 1102 96-97 -0.19846210299722955 1102 98-99 -0.17244120654656214 1102 100-101 -0.626916802464347 1103 1 0.20863078035278448 1103 2 0.23118956537502555 1103 3 0.1551609295427241 1103 4 0.08262069932599303 1103 5 0.08696594235615862 1103 6 0.043235164543297344 1103 7 0.07082576319211142 1103 8 0.14456166723845598 1103 9 0.17924297560850277 1103 10-11 0.20844092881518606 1103 12-13 0.18653604663854395 1103 14-15 0.21663866437213386 1103 16-17 0.23525161592551314 1103 18-19 0.2362449254771093 1103 20-21 0.2745615468909435 1103 22-23 0.2840526477099772 1103 24-25 0.24262279258434205 1103 26-27 0.34368720983393786 1103 28-29 0.3117643361742566 1103 30-31 0.19796347317717533 1103 32-33 0.1953821706482941 1103 34-35 0.1969108593680744 1103 36-37 0.14781432584418752 1103 38-39 0.20033855560382818 1103 40-41 0.21222587166014506 1103 42-43 0.1412675561906127 1103 44-45 0.21499677712219523 1103 46-47 0.05681407800588545 1103 48-49 -0.05971404192009544 1103 50-51 -0.11196177396454488 1103 52-53 -0.23747859920757008 1103 54-55 -0.12633727368436354 1103 56-57 -0.08878936498858536 1103 58-59 -0.10541872790711437 1103 60-61 -0.14899579001976093 1103 62-63 -0.2367174422774596 1103 64-65 -0.20280986074192953 1103 66-67 -0.3131869443370796 1103 68-69 -0.14554515930169387 1103 70-71 -0.42934105298905934 1103 72-73 -0.5913585688555436 1103 74-75 -0.5493770137436798 1103 76-77 -0.3269000887208122 1103 78-79 -0.5267915323415764 1103 80-81 -0.5395989459824975 1103 82-83 -0.48202052772234083 1103 84-85 -0.7311353816148838 1103 86-87 -0.6419655587064561 1103 88-89 -1.0381978037134267 1103 90-91 -0.9607277718224587 1103 92-93 -0.7681988618302533 1103 94-95 -0.7951441250588935 1103 96-97 -0.8183588193569662 1103 98-99 -0.6796213346935396 1103 100-101 -1.1690156375834704 1104 1 0.152000774862465 1104 2 0.1323726410174828 1104 3 0.14345414811063506 1104 4 0.08842981325901178 1104 5 0.08705449592221015 1104 6 0.11070412651839945 1104 7 0.08361289813003481 1104 8 0.07219569306067086 1104 9 0.11943389709691132 1104 10-11 0.1897871201263115 1104 12-13 0.17822972214285926 1104 14-15 0.16386959436169235 1104 16-17 0.07620498361762884 1104 18-19 0.11841997816648586 1104 20-21 0.244572881747402 1104 22-23 0.15418884309456615 1104 24-25 0.17430371637514241 1104 26-27 0.21678994968126375 1104 28-29 0.16487168080703185 1104 30-31 0.18065125101399104 1104 32-33 0.15347862319243433 1104 34-35 0.22351235061012176 1104 36-37 0.09702442803499878 1104 38-39 0.023364253848690453 1104 40-41 0.20466339711929038 1104 42-43 0.18925473363424317 1104 44-45 0.07281074379064023 1104 46-47 -0.06975553395238876 1104 48-49 -0.13250507321492933 1104 50-51 -0.06359824386518653 1104 52-53 -0.12221284995583659 1104 54-55 -0.11881907592654528 1104 56-57 -0.06961309026000606 1104 58-59 -0.009059165007514025 1104 60-61 -0.3071126098841006 1104 62-63 -0.2538614126651453 1104 64-65 -0.1978375780081052 1104 66-67 -0.5207520754849106 1104 68-69 -0.601277231632249 1104 70-71 -0.6674217429984495 1104 72-73 -0.7229668787221861 1104 74-75 -0.6148136713778811 1104 76-77 -0.5783922163087887 1104 78-79 -0.768316956070386 1104 80-81 -0.7474740146115124 1104 82-83 -0.8300626083769274 1104 84-85 -0.8470032951157584 1104 86-87 -0.7898942907964965 1104 88-89 -0.7509698845451211 1104 90-91 -0.7850729182015037 1104 92-93 -0.9700124388630051 1104 94-95 -0.7623350288365884 1104 96-97 -0.8842293894648279 1104 98-99 -1.0433019751129287 1104 100-101 -1.4038198456489308 1105 1 0.06928921785360131 1105 2 0.08963305956535805 1105 3 0.1077012089382805 1105 4 0.03537882491067279 1105 5 -0.021434425585013628 1105 6 0.09070661131821112 1105 7 0.09808467964184331 1105 8 0.11548910308978577 1105 9 0.1013197064564082 1105 10-11 0.07558328713047757 1105 12-13 0.129826462187971 1105 14-15 0.058239729629576686 1105 16-17 0.11045141684119386 1105 18-19 0.1522901051090102 1105 20-21 0.23059114374520817 1105 22-23 0.23504064271723735 1105 24-25 0.1399012787096794 1105 26-27 0.20861162815073442 1105 28-29 0.15597955570120092 1105 30-31 0.0910666608714692 1105 32-33 0.2283929040029946 1105 34-35 0.1679933412511474 1105 36-37 0.25001637036715607 1105 38-39 0.18117017561336723 1105 40-41 0.18220400886676202 1105 42-43 0.22390526703367186 1105 44-45 0.18488879212127785 1105 46-47 0.03362622736081278 1105 48-49 -0.0952070752719365 1105 50-51 -0.03246498302652867 1105 52-53 0.055983800175376075 1105 54-55 0.1956917707369783 1105 56-57 0.06254585495528886 1105 58-59 0.09298977017891019 1105 60-61 -0.061814709283773084 1105 62-63 -0.20903690485403814 1105 64-65 -0.25229260633354045 1105 66-67 -0.25272094632271447 1105 68-69 -0.37248903263820665 1105 70-71 -0.44956205221513557 1105 72-73 -0.5155551307423636 1105 74-75 -0.28513414651258984 1105 76-77 -0.43549406876547536 1105 78-79 -0.4206742497903768 1105 80-81 -0.5913888807589309 1105 82-83 -0.29801163667931263 1105 84-85 -0.3489488677532435 1105 86-87 -0.38359737258867455 1105 88-89 -0.6653695556293542 1105 90-91 -0.9612189680742524 1105 92-93 -0.7276067683961287 1105 94-95 -0.6823727619306617 1105 96-97 -0.9440257127295979 1105 98-99 -1.3065667715194458 1105 100-101 -1.7709185344687413 1106 1 0.20770532214263682 1106 2 0.18982127386373548 1106 3 0.20139174562972784 1106 4 0.11245450065023732 1106 5 0.13048228737193313 1106 6 0.11974982082997343 1106 7 0.10311032642535878 1106 8 0.15864720310042202 1106 9 0.09101430881307238 1106 10-11 0.17152412224647406 1106 12-13 0.0402891975297095 1106 14-15 0.19511510635364715 1106 16-17 0.22409473744962582 1106 18-19 0.2771408928316532 1106 20-21 0.27091140352230525 1106 22-23 0.2591698930511157 1106 24-25 0.2736869271478213 1106 26-27 0.2238679187039736 1106 28-29 0.2101827621400858 1106 30-31 0.28727947599007564 1106 32-33 0.36722768907822 1106 34-35 0.3140525120445403 1106 36-37 0.3470760020935231 1106 38-39 0.34792375966271294 1106 40-41 0.4175101451847354 1106 42-43 0.314578022349167 1106 44-45 0.3059414625778345 1106 46-47 0.32392979309103964 1106 48-49 0.35238661821944817 1106 50-51 0.30253238729174825 1106 52-53 0.3100051449177883 1106 54-55 -0.03373481091316677 1106 56-57 -0.10407521086358429 1106 58-59 0.21403180372065123 1106 60-61 0.047316491151157436 1106 62-63 0.12554961462924297 1106 64-65 -0.211093175999423 1106 66-67 -0.08617049779947195 1106 68-69 -0.012966925583565825 1106 70-71 -0.5630434553423882 1106 72-73 -0.39812196602382954 1106 74-75 -0.12090564785402336 1106 76-77 -0.22233723927521964 1106 78-79 -0.5177257560957038 1106 80-81 -0.5338793666295629 1106 82-83 -0.4676199027440049 1106 84-85 -0.6383874454795446 1106 86-87 -0.6729885274030636 1106 88-89 -0.7668816226182784 1106 90-91 -0.8983444600315629 1106 92-93 -0.8067254704561471 1106 94-95 -0.8540218382918923 1106 96-97 -1.2841422460408722 1106 98-99 -1.4282393377342402 1106 100-101 -1.876451059790508 1107 1 0.07465514532302109 1107 2 0.07154557794518723 1107 3 0.04483613909782491 1107 4 0.06875393276401098 1107 5 0.04688343192498934 1107 6 0.10246161473139637 1107 7 0.09113055037492757 1107 8 0.08320251987205296 1107 9 0.1003508456071387 1107 10-11 0.11007683556601222 1107 12-13 0.008672538289978604 1107 14-15 0.052650876380575085 1107 16-17 0.05276492441085878 1107 18-19 0.12351362112212882 1107 20-21 0.020985352757186604 1107 22-23 0.03491959286387214 1107 24-25 0.09114660217728954 1107 26-27 0.12824099382871168 1107 28-29 0.026208453119537012 1107 30-31 0.004142124135498193 1107 32-33 -0.016698628398053472 1107 34-35 -0.020988696475292556 1107 36-37 0.024040088284031924 1107 38-39 -0.02804855615841717 1107 40-41 -0.08648023235972602 1107 42-43 -0.194674961413277 1107 44-45 -0.2514701715164165 1107 46-47 -0.42774812739611434 1107 48-49 -0.4676387797070092 1107 50-51 -0.5069029039721968 1107 52-53 -0.3839059381929175 1107 54-55 -0.3240467477190876 1107 56-57 -0.4201946074597771 1107 58-59 -0.2564923605295135 1107 60-61 -0.49561522420767545 1107 62-63 -0.38112255455563115 1107 64-65 -0.5191761154856209 1107 66-67 -0.5616209981847184 1107 68-69 -0.30007846572524244 1107 70-71 -0.6160374801344517 1107 72-73 -0.7573183697037855 1107 74-75 -0.7366167184523604 1107 76-77 -0.6954140926982326 1107 78-79 -0.628181766316338 1107 80-81 -0.5714462639336979 1107 82-83 -0.7843391250450082 1107 84-85 -0.8340342090549235 1107 86-87 -0.9140292826236873 1107 88-89 -0.9096937844274358 1107 90-91 -1.1893759956073175 1107 92-93 -1.283232525679626 1107 94-95 -1.4157045551895244 1107 96-97 -1.6604749786338502 1107 98-99 -1.8958145875975916 1107 100-101 -2.096565279117076 1108 1 -0.018868268614561146 1108 2 -0.0677982469609546 1108 3 -0.07438849767077471 1108 4 0.022635338005812855 1108 5 -0.030767084720579874 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0.7156041326304816 2316 74-75 0.910991668734539 2316 76-77 0.8528684749219906 2316 78-79 0.7310600931571116 2316 80-81 0.9084661734822141 2316 82-83 1.0376941502073507 2316 84-85 1.0841304321296334 2316 86-87 1.3174050233821788 2316 88-89 1.4854075631982298 2316 90-91 1.3230342178218848 2316 92-93 1.4290993887134285 2316 94-95 1.8962160135629098 2316 96-97 2.270831402186193 2316 98-99 2.408314675656264 2316 100-101 2.889449336703006 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 3.0 4 10.0 5 30.0 6 59.0 7 195.0 8 530.0 9 954.0 10 1677.0 11 2774.0 12 4319.0 13 6427.0 14 8739.0 15 12194.0 16 15740.0 17 21319.0 18 21994.0 19 18594.0 20 17964.0 21 18883.0 22 20398.0 23 22266.0 24 25056.0 25 28745.0 26 32099.0 27 37909.0 28 45399.0 29 54398.0 30 65019.0 31 78499.0 32 97293.0 33 123407.0 34 162258.0 35 223483.0 36 301486.0 37 344889.0 38 166180.0 39 6579.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.487453556146434 19.068984687678448 15.391612560782647 23.051949195392467 2 15.54970118208924 19.9054791501677 40.16391463035435 24.380905037388718 3 19.54111989567202 23.407534194884427 30.057436739589594 26.993909169853964 4 13.065847658431306 17.024454539509097 36.942754946497836 32.96694285556176 5 13.735667226092591 37.15166680752931 34.475276593860805 14.637389372517296 6 32.316730826892154 36.33246333006663 16.969138696765963 14.381667146275253 7 28.501723790114657 32.62332532268556 20.26777732445035 18.60717356274943 8 26.191799759630257 35.106659670555615 19.328685245936278 19.372855323877854 9 25.569997751250018 17.148705761377943 20.329051988382968 36.95224449898907 10-11 24.907748427761547 26.59805883072044 27.046702059744305 21.447490681773708 12-13 25.939999129671193 23.77139631679868 27.49708276349016 22.79152179003997 14-15 22.459151481735066 25.44837911409312 26.10773575024487 25.984733653926934 16-17 21.94652422558984 28.518165890862534 27.023741996179044 22.51156788736858 18-19 21.878928709594817 27.099726276316538 29.253671574844386 21.76767343924426 20-21 22.348323826034285 26.122425571154828 29.771764453752468 21.757486149058415 22-23 22.120455525309506 26.08605317212093 29.61845202490629 22.175039277663274 24-25 22.127584645570256 26.192861095738795 29.627081551541984 22.052472707148965 26-27 21.71903535247946 26.52222162680427 29.587684811231963 22.171058209484304 28-29 21.735306821100412 27.066631751036464 29.334562926996398 21.863498500866726 30-31 21.960239592776798 26.461735286808008 29.285014987125862 22.29301013328933 32-33 21.73313726782411 26.444570325253764 29.329736674898665 22.492555732023458 34-35 22.473385555561983 26.587250776126233 29.10627439033747 21.833089277974317 36-37 22.362595264315242 26.226770951609325 29.2217265775177 22.188907206557737 38-39 21.91181042783735 26.657252185672213 29.079074597100796 22.351862789389642 40-41 22.42753945818788 26.436721759468178 29.045115123379002 22.090623658964944 42-43 22.06741010024581 26.47008070577558 28.889065529548013 22.5734436644306 44-45 22.17610131551907 26.675262561749456 28.974345881478776 22.1742902412527 46-47 22.573064935345872 26.87210463788684 28.34350260430095 22.211327822466338 48-49 22.34072818238643 26.93012100319078 28.292912993662455 22.43623782076033 50-51 22.30900074508563 27.19972571189385 28.346533741914616 22.144739801105906 52-53 22.686864453573776 27.046043477834314 28.049836726058754 22.217255342533157 54-55 22.255382926474635 27.13036701554165 28.24740255591215 22.366847502071565 56-57 22.47844467567388 27.131053755476884 28.054501158271627 22.336000410577604 58-59 22.47784947708261 26.930094519632547 28.021694630737397 22.57036137254744 60-61 22.157683675764112 27.208569095653722 28.280561311786833 22.353185916795333 62-63 22.185434628921925 27.166603350634123 28.362471287646834 22.28549073279712 64-65 21.916255254142367 27.311677976894476 28.151183441554767 22.62088332740839 66-67 21.946257431401673 27.184464798199958 27.985264994626448 22.884012775771918 68-69 22.29913851625919 26.92963249432731 28.21357993068475 22.557649058728742 70-71 22.25716662062761 27.026561264901684 27.798709195685106 22.917562918785595 72-73 22.262389326992906 27.009534034473052 27.90674098824829 22.82133565028575 74-75 22.115493713903025 27.500633351975388 27.63321587075645 22.750657063365136 76-77 22.219300982207553 27.006883503743662 27.943502938571775 22.830312575477016 78-79 22.330752230201924 27.16392990563911 27.918887475443626 22.58643038871534 80-81 22.472810664820503 27.24656661688999 27.55579247553765 22.72483024275186 82-83 22.372504684025195 27.241374624887964 27.787831649868927 22.59828904121791 84-85 22.376907360084573 27.38499734191702 27.67007768445624 22.56801761354216 86-87 22.468526580489776 27.37401196586272 27.43278952350423 22.724671930143277 88-89 22.31856514129023 27.480651955082795 27.20596393530969 22.994818968317286 90-91 22.363370712844187 27.405415428867176 27.327744480443755 22.903469377844885 92-93 22.39271575414412 27.164586166424087 27.461621972497895 22.981076106933894 94-95 22.186194015957845 27.052467287819887 27.545653151770626 23.215685544451635 96-97 22.298141943099285 26.818314352424764 27.640058677334782 23.243485027141162 98-99 22.150531521085878 27.028344688600363 27.170529755093014 23.65059403522074 100-101 22.635340890823922 26.312303323992758 27.295944358032152 23.75641142715117 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 519.0 1 369.5 2 296.0 3 368.5 4 287.5 5 289.0 6 571.5 7 1313.0 8 2213.0 9 2606.0 10 2238.0 11 1674.5 12 1608.0 13 1798.5 14 1957.0 15 2121.5 16 2244.0 17 2488.5 18 2737.5 19 3036.5 20 3484.5 21 4192.0 22 5292.5 23 6474.0 24 8064.5 25 10222.0 26 12778.0 27 15734.5 28 18644.0 29 21557.5 30 24583.0 31 28433.0 32 33608.5 33 36406.5 34 37695.0 35 40566.5 36 44369.0 37 48431.0 38 51443.0 39 53819.5 40 56657.0 41 59895.0 42 62266.5 43 64565.0 44 67546.0 45 70526.0 46 71913.0 47 74871.0 48 78003.0 49 76031.0 50 72445.5 51 69736.5 52 67850.5 53 67642.5 54 67710.0 55 63484.0 56 57803.0 57 52620.0 58 48301.5 59 43098.0 60 35062.5 61 29029.5 62 24279.0 63 18314.5 64 13176.0 65 9931.0 66 7435.0 67 5670.0 68 4329.5 69 3247.0 70 2406.5 71 1643.0 72 1168.5 73 915.0 74 654.0 75 451.5 76 295.5 77 179.0 78 131.0 79 82.5 80 47.0 81 30.0 82 18.0 83 12.5 84 7.0 85 5.5 86 3.5 87 2.0 88 1.0 89 1.0 90 1.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.007847986513537021 2 0.013733976398689788 3 0.009709367930209264 4 5.0307605856006556E-5 5 9.558445112641244E-4 6 0.0019619966283842554 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 8.30075496624108E-4 14-15 0.0 16-17 0.0024399188840163177 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.003571840015776465 26-27 0.018689275575506436 28-29 0.006162681717360803 30-31 0.003269994380640426 32-33 0.0 34-35 0.00882898482772915 36-37 6.036912702720786E-4 38-39 0.0026663031103683473 40-41 0.0017104585991042228 42-43 0.0028675335337923734 44-45 2.5153802928003278E-5 46-47 7.546140878400983E-5 48-49 6.791526790560884E-4 50-51 0.004024608468480524 52-53 0.008074370739889052 54-55 0.007244295243264944 56-57 0.01645058711491414 58-59 0.02309119108790701 60-61 0.0035215324099204586 62-63 0.019418735860418528 64-65 0.020651272203890687 66-67 0.013029669916705698 68-69 0.03109010041901205 70-71 0.016500894720770147 72-73 0.005156529600240671 74-75 0.01657635612955416 76-77 0.020676426006818693 78-79 0.02550595616899532 80-81 0.011419826529313488 82-83 0.035114708887492574 84-85 0.02195926995614686 86-87 0.03109010041901205 88-89 0.021405886291730788 90-91 0.02724156857102755 92-93 0.02356911334353907 94-95 0.014337667668961867 96-97 0.01466466710702591 98-99 0.011092827091249445 100-101 0.010388520609265353 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1987771.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.33520473989443 #Duplication Level Percentage of deduplicated Percentage of total 1 77.50141200403291 45.98562148890205 2 13.33710673861262 15.82719917946811 3 4.161725690322233 7.408105377196446 4 1.7723250635893222 4.2064508205487545 5 0.9407747121724467 2.791053008043369 6 0.519845974319478 1.8507100391673679 7 0.36063417389694247 1.497881178106444 8 0.24340440294429336 1.1553960066633127 9 0.1759814981150482 0.9397708398980755 >10 0.9041210314366687 9.526906514707886 >50 0.050409877507711025 2.0401900115812768 >100 0.027068364156859592 3.087708128179679 >500 0.0035737654676340517 1.5098977289275628 >1k 0.0014465241178518698 1.446888554658126 >5k 1.7017930798257291E-4 0.7262211239515138 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7619 0.38329364901691393 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6765 0.34033095361588434 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4177 0.21013486966053938 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2465 0.12400824843505616 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 7.546140878400982E-5 0.0 12-13 0.0 0.0 0.0 1.006152117120131E-4 0.0 14-15 0.0 0.0 0.0 1.006152117120131E-4 2.5153802928003275E-5 16-17 0.0 0.0 0.0 1.006152117120131E-4 5.030760585600655E-5 18-19 0.0 0.0 0.0 1.006152117120131E-4 5.030760585600655E-5 20-21 0.0 5.030760585600655E-5 0.0 1.5092281756801966E-4 7.546140878400982E-5 22-23 0.0 5.030760585600655E-5 0.0 2.012304234240262E-4 1.006152117120131E-4 24-25 5.030760585600655E-5 5.030760585600655E-5 0.0 3.2699943806404263E-4 1.006152117120131E-4 26-27 5.030760585600655E-5 5.030760585600655E-5 0.0 7.546140878400983E-4 1.5092281756801966E-4 28-29 1.2576901464001638E-4 5.030760585600655E-5 0.0 0.0034712248040644524 1.5092281756801966E-4 30-31 2.012304234240262E-4 5.030760585600655E-5 0.0 0.011243749908817463 1.5092281756801966E-4 32-33 2.012304234240262E-4 5.030760585600655E-5 0.0 0.02847410491449971 2.012304234240262E-4 34-35 2.012304234240262E-4 5.030760585600655E-5 0.0 0.05312483178394292 2.012304234240262E-4 36-37 2.5153802928003276E-4 5.030760585600655E-5 0.0 0.09319483984825214 2.012304234240262E-4 38-39 2.5153802928003276E-4 1.006152117120131E-4 0.0 0.1511743555972997 2.2638422635202948E-4 40-41 2.5153802928003276E-4 2.012304234240262E-4 0.0 0.20988333163125933 2.5153802928003276E-4 42-43 2.5153802928003276E-4 2.2638422635202948E-4 0.0 0.26901992231499505 2.5153802928003276E-4 44-45 2.5153802928003276E-4 2.5153802928003276E-4 0.0 0.34420463926679684 2.5153802928003276E-4 46-47 3.2699943806404263E-4 2.5153802928003276E-4 0.0 0.42977787682786395 3.018456351360393E-4 48-49 3.521532409920459E-4 3.018456351360393E-4 0.0 0.5217904879384999 4.024608468480524E-4 50-51 3.521532409920459E-4 3.018456351360393E-4 0.0 0.6174504004736965 4.024608468480524E-4 52-53 3.521532409920459E-4 3.018456351360393E-4 0.0 0.7087083974964923 4.2761464977605565E-4 54-55 3.521532409920459E-4 3.521532409920459E-4 0.0 0.8085941489235933 4.5276845270405896E-4 56-57 3.521532409920459E-4 4.024608468480524E-4 0.0 0.929256941569225 5.030760585600655E-4 58-59 3.521532409920459E-4 4.2761464977605565E-4 0.0 1.0495675809738647 5.53383664416072E-4 60-61 3.521532409920459E-4 5.030760585600655E-4 0.0 1.18127289310489 5.53383664416072E-4 62-63 3.521532409920459E-4 5.030760585600655E-4 0.0 1.3059351404160742 6.036912702720786E-4 64-65 3.521532409920459E-4 5.030760585600655E-4 0.0 1.4374140683207473 6.036912702720786E-4 66-67 3.521532409920459E-4 5.030760585600655E-4 0.0 1.5928897242187354 6.288450732000819E-4 68-69 3.521532409920459E-4 5.030760585600655E-4 0.0 1.7635582770852376 6.539988761280852E-4 70-71 4.024608468480524E-4 5.030760585600655E-4 0.0 1.9454454260576295 6.791526790560884E-4 72-73 4.2761464977605565E-4 5.030760585600655E-4 0.0 2.1231067361381166 7.546140878400983E-4 74-75 4.5276845270405896E-4 5.030760585600655E-4 0.0 2.3034846569348284 7.546140878400983E-4 76-77 4.5276845270405896E-4 5.030760585600655E-4 0.0 2.4990051670941975 7.546140878400983E-4 78-79 4.5276845270405896E-4 5.030760585600655E-4 0.0 2.7138437979022734 7.546140878400983E-4 80-81 4.5276845270405896E-4 5.030760585600655E-4 0.0 2.94581216850432 7.546140878400983E-4 82-83 4.5276845270405896E-4 5.030760585600655E-4 0.0 3.179390382493758 7.546140878400983E-4 84-85 4.5276845270405896E-4 5.030760585600655E-4 0.0 3.416012206637485 7.546140878400983E-4 86-87 4.5276845270405896E-4 5.030760585600655E-4 0.0 3.6523070313431476 7.546140878400983E-4 88-89 4.5276845270405896E-4 5.030760585600655E-4 0.0 3.909278282055629 7.546140878400983E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6145 0.0 33.701202 1 GTATCAA 8645 0.0 30.383722 1 ATCAACG 10360 0.0 25.212633 3 TCAACGC 10430 0.0 25.088324 4 TATCAAC 10510 0.0 24.854046 2 CAACGCA 10585 0.0 24.720945 5 AACGCAG 10865 0.0 24.127577 6 GTACATG 5555 0.0 22.146063 1 GAGTACT 7400 0.0 22.14072 12-13 TACATGG 5360 0.0 21.886147 2 ACGCAGA 12095 0.0 21.516327 7 CGCAGAG 12100 0.0 21.389694 8 TAAGGTG 1115 0.0 20.870152 5 GTACTTT 7865 0.0 20.771318 14-15 AGAGTAC 9915 0.0 20.667454 10-11 ACATGGG 5425 0.0 20.659868 3 TATACCG 115 0.003728656 20.64793 5 GTAAGGT 1100 0.0 19.859554 4 GTGTAGC 1620 0.0 19.644484 1 AGTACTT 7805 0.0 19.379425 12-13 >>END_MODULE